fluorochrome conjugated antibodies against il 17a  (Thermo Fisher)


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    Thermo Fisher fluorochrome conjugated antibodies against il 17a
    γδ T cells are the primary source of IL-17 during S. aureus induced peritonitis Mice were infected with S. aureus (5×10 8 CFU) via i.p. injection. At the indicated times post–infection, the peritoneal cavity was lavaged and the MLN collected. Secreted <t>IL-17A</t> and IL-1β in the peritoneal fluid was measured by ELISA (A). 3 h post-infection (B, C), and at the indicated time-points (D E), PECs (B D) and MLN cells (C E), cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3, CD4, CD8 and γδTCR, and intracellular IL-17, and analysed by flow cytometry. IL-1RI −/− and WT mice were infected with S. aureus (5×10 8 CFU) via i.p. injection and at 3 h post-infection PECs, cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3 and γδTCR, and intracellular IL-17, and analysed by flow cytometry (F). Results expressed as mean ± SEM of n=10 mice/ group, with representative FACS plots. *
    Fluorochrome Conjugated Antibodies Against Il 17a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorochrome conjugated antibodies against il 17a/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorochrome conjugated antibodies against il 17a - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Staphylococcus aureus infection of mice expands a population of memory γδ T cells that are protective against subsequent infection"

    Article Title: Staphylococcus aureus infection of mice expands a population of memory γδ T cells that are protective against subsequent infection

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1303420

    γδ T cells are the primary source of IL-17 during S. aureus induced peritonitis Mice were infected with S. aureus (5×10 8 CFU) via i.p. injection. At the indicated times post–infection, the peritoneal cavity was lavaged and the MLN collected. Secreted IL-17A and IL-1β in the peritoneal fluid was measured by ELISA (A). 3 h post-infection (B, C), and at the indicated time-points (D E), PECs (B D) and MLN cells (C E), cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3, CD4, CD8 and γδTCR, and intracellular IL-17, and analysed by flow cytometry. IL-1RI −/− and WT mice were infected with S. aureus (5×10 8 CFU) via i.p. injection and at 3 h post-infection PECs, cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3 and γδTCR, and intracellular IL-17, and analysed by flow cytometry (F). Results expressed as mean ± SEM of n=10 mice/ group, with representative FACS plots. *
    Figure Legend Snippet: γδ T cells are the primary source of IL-17 during S. aureus induced peritonitis Mice were infected with S. aureus (5×10 8 CFU) via i.p. injection. At the indicated times post–infection, the peritoneal cavity was lavaged and the MLN collected. Secreted IL-17A and IL-1β in the peritoneal fluid was measured by ELISA (A). 3 h post-infection (B, C), and at the indicated time-points (D E), PECs (B D) and MLN cells (C E), cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3, CD4, CD8 and γδTCR, and intracellular IL-17, and analysed by flow cytometry. IL-1RI −/− and WT mice were infected with S. aureus (5×10 8 CFU) via i.p. injection and at 3 h post-infection PECs, cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3 and γδTCR, and intracellular IL-17, and analysed by flow cytometry (F). Results expressed as mean ± SEM of n=10 mice/ group, with representative FACS plots. *

    Techniques Used: Mouse Assay, Infection, Injection, Enzyme-linked Immunosorbent Assay, Cell Culture, Staining, Flow Cytometry, Cytometry, FACS

    2) Product Images from "Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection"

    Article Title: Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1005226

    Human S . aureus bloodstream infection is associated with increased IFNγ production. Serum from S . aureus and E . coli bloodstream infection patients was collected on day 7 ± 2 post-initial bacteraemia and assessed for IFNγ (A) and IL-17A (B) by ELISA. Results expressed as individual patient values with median indicated by bar, n = 11–24 per group. *p
    Figure Legend Snippet: Human S . aureus bloodstream infection is associated with increased IFNγ production. Serum from S . aureus and E . coli bloodstream infection patients was collected on day 7 ± 2 post-initial bacteraemia and assessed for IFNγ (A) and IL-17A (B) by ELISA. Results expressed as individual patient values with median indicated by bar, n = 11–24 per group. *p

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

    Human S . aureus bloodstream infection induces S . aureus antigen-specific Th1 cells. PBMCs were isolated from patients, CFSE-labelled and incubated with heat-killed S . aureus PS80 strain (1μg/ml ≈ 1x10 7 CFU/ml) or media alone for 10 d before assessing S . aureus antigen-specific proliferation by gating on CFSE lo cells of the CD4 + population using flow cytometry (A). S . aureus antigen-specific Th1 and cytotoxic T cell division was assessed by gating on CFSE lo IFNγ + cells of the CD4 + and CD8 + populations respectively (B). S . aureus antigen-specific Th1 and Th17 proportions were compared by gating on CFSE lo IFNγ + or IL-17A + cells in the CD4 + population (C). For each patient, media only responses were subtracted from responses to heat-killed S . aureus to determine the antigen-specific response. Results shown as box-and-whiskers plots where the horizontal line indicates the median, boundaries of the box indicate the IQR, and whiskers indicate the highest and lowest values of the results, and representative FACS plots of CD4 + or CD8 + lymphocytes (A, B). n = 5–17 per group. SA = S . aureus , EC = E . coli , BSI = bloodstream infection. *p
    Figure Legend Snippet: Human S . aureus bloodstream infection induces S . aureus antigen-specific Th1 cells. PBMCs were isolated from patients, CFSE-labelled and incubated with heat-killed S . aureus PS80 strain (1μg/ml ≈ 1x10 7 CFU/ml) or media alone for 10 d before assessing S . aureus antigen-specific proliferation by gating on CFSE lo cells of the CD4 + population using flow cytometry (A). S . aureus antigen-specific Th1 and cytotoxic T cell division was assessed by gating on CFSE lo IFNγ + cells of the CD4 + and CD8 + populations respectively (B). S . aureus antigen-specific Th1 and Th17 proportions were compared by gating on CFSE lo IFNγ + or IL-17A + cells in the CD4 + population (C). For each patient, media only responses were subtracted from responses to heat-killed S . aureus to determine the antigen-specific response. Results shown as box-and-whiskers plots where the horizontal line indicates the median, boundaries of the box indicate the IQR, and whiskers indicate the highest and lowest values of the results, and representative FACS plots of CD4 + or CD8 + lymphocytes (A, B). n = 5–17 per group. SA = S . aureus , EC = E . coli , BSI = bloodstream infection. *p

    Techniques Used: Infection, Isolation, Incubation, Flow Cytometry, Cytometry, FACS

    Related Articles

    Staining:

    Article Title: CD147 Expressed on Memory CD4+ T Cells Limits Th17 Responses in Patients With Rheumatoid Arthritis
    Article Snippet: .. Phenotypic AnalysisThe following anti-human monoclonal antibodies were used for surface phenotype and intracellular cytokine staining: anti-CD4-fluorescein isothiocyanate (FITC); anti-CD4-phycoerythrin (PE); anti-CD161-PE; anti-CCR6-PE; anti-CD45RO-PE; anti-CD147-peridinin chlorophylla protein cyanine 5.5 dies (Percp–cy5.5); anti-interferon (IFN)-γ-FITC (all from BD Biosciences); and anti-IL-17A-allophycocyanin (APC; eBiosciences). ..

    Article Title: Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
    Article Snippet: .. After overnight incubation, cells were collected, surface stained, permeabilized, and intracellularly stained with the following antibodies: TNF-α-AF700 (Mab11; eBioscience); IL-17A-PE (ebio64CAP17; eBioscience); IL-22-PerCP-eFluor710 (IL22JOP; eBioscience); IFN-γ-BV650 (4S.B3; BioLegend); IL-10-PE-Cy7 (JES3-9D7; BioLegend); IL-21-BV421 (3A3-N2.1; BD Biosciences). ..

    Flow Cytometry:

    Article Title: Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
    Article Snippet: .. The following antibodies were used for flow cytometry: CD3e-PerCP-Cyanine5.5; CD4-FITC; CD25-APC; IFN-γ-PE; IL-4-PE; IL-17A-PE; Foxp3-PE; PD-1-PE; KLRG1-PE (all from eBioscience, San Diego, CA, USA); CXCR5-PerCP-Cyanine5.5 (BD Pharmingen, San Jose, CA, USA); ICOS-BV421 (BD Pharmingen); and CCR4-PE (BioLegend, San Diego, CA, USA). ..

    Article Title: Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica.
    Article Snippet: .. The following antibodies were used for flow cytometry: CD3e-PerCP-Cyanine5.5; CD4-FITC; CD25-APC; IFN-γ-PE; IL-4-PE; IL-17A-PE; Foxp3-PE; PD-1-PE; KLRG1-PE (all from eBioscience, Fig. 1 Hepatic pathology was detected after fucoidan treatment during S. japonicum infection. a Experimental procedure of S. japonicum-infected model. ..

    Article Title: Dimethyl Fumarate Suppresses Demyelination and Axonal Loss through Reduction in Pro-Inflammatory Macrophage-Induced Reactive Astrocytes and Complement C3 Deposition
    Article Snippet: .. Antibodies against F4/80, iNOS, and CD206 for flow cytometry and ELISA kits for IFN-γ, IL-17A, GM-CSF and IL-10 were purchased from eBioscience, San Diego, CA, USA. ..

    Infection:

    Article Title: Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica.
    Article Snippet: .. The following antibodies were used for flow cytometry: CD3e-PerCP-Cyanine5.5; CD4-FITC; CD25-APC; IFN-γ-PE; IL-4-PE; IL-17A-PE; Foxp3-PE; PD-1-PE; KLRG1-PE (all from eBioscience, Fig. 1 Hepatic pathology was detected after fucoidan treatment during S. japonicum infection. a Experimental procedure of S. japonicum-infected model. ..

    Incubation:

    Article Title: Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques
    Article Snippet: .. After overnight incubation, cells were collected, surface stained, permeabilized, and intracellularly stained with the following antibodies: TNF-α-AF700 (Mab11; eBioscience); IL-17A-PE (ebio64CAP17; eBioscience); IL-22-PerCP-eFluor710 (IL22JOP; eBioscience); IFN-γ-BV650 (4S.B3; BioLegend); IL-10-PE-Cy7 (JES3-9D7; BioLegend); IL-21-BV421 (3A3-N2.1; BD Biosciences). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Dimethyl Fumarate Suppresses Demyelination and Axonal Loss through Reduction in Pro-Inflammatory Macrophage-Induced Reactive Astrocytes and Complement C3 Deposition
    Article Snippet: .. Antibodies against F4/80, iNOS, and CD206 for flow cytometry and ELISA kits for IFN-γ, IL-17A, GM-CSF and IL-10 were purchased from eBioscience, San Diego, CA, USA. ..

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    Thermo Fisher fluorochrome conjugated antibodies against il 17a
    γδ T cells are the primary source of IL-17 during S. aureus induced peritonitis Mice were infected with S. aureus (5×10 8 CFU) via i.p. injection. At the indicated times post–infection, the peritoneal cavity was lavaged and the MLN collected. Secreted <t>IL-17A</t> and IL-1β in the peritoneal fluid was measured by ELISA (A). 3 h post-infection (B, C), and at the indicated time-points (D E), PECs (B D) and MLN cells (C E), cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3, CD4, CD8 and γδTCR, and intracellular IL-17, and analysed by flow cytometry. IL-1RI −/− and WT mice were infected with S. aureus (5×10 8 CFU) via i.p. injection and at 3 h post-infection PECs, cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3 and γδTCR, and intracellular IL-17, and analysed by flow cytometry (F). Results expressed as mean ± SEM of n=10 mice/ group, with representative FACS plots. *
    Fluorochrome Conjugated Antibodies Against Il 17a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorochrome conjugated antibodies against il 17a/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorochrome conjugated antibodies against il 17a - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    95
    Thermo Fisher fluorochrome conjugated antibodies against il 2 jes6 5h4 il 17a
    γδ T cells are the primary source of IL-17 during S. aureus induced peritonitis Mice were infected with S. aureus (5×10 8 CFU) via i.p. injection. At the indicated times post–infection, the peritoneal cavity was lavaged and the MLN collected. Secreted <t>IL-17A</t> and IL-1β in the peritoneal fluid was measured by ELISA (A). 3 h post-infection (B, C), and at the indicated time-points (D E), PECs (B D) and MLN cells (C E), cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3, CD4, CD8 and γδTCR, and intracellular IL-17, and analysed by flow cytometry. IL-1RI −/− and WT mice were infected with S. aureus (5×10 8 CFU) via i.p. injection and at 3 h post-infection PECs, cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3 and γδTCR, and intracellular IL-17, and analysed by flow cytometry (F). Results expressed as mean ± SEM of n=10 mice/ group, with representative FACS plots. *
    Fluorochrome Conjugated Antibodies Against Il 2 Jes6 5h4 Il 17a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorochrome conjugated antibodies against il 2 jes6 5h4 il 17a/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorochrome conjugated antibodies against il 2 jes6 5h4 il 17a - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    γδ T cells are the primary source of IL-17 during S. aureus induced peritonitis Mice were infected with S. aureus (5×10 8 CFU) via i.p. injection. At the indicated times post–infection, the peritoneal cavity was lavaged and the MLN collected. Secreted IL-17A and IL-1β in the peritoneal fluid was measured by ELISA (A). 3 h post-infection (B, C), and at the indicated time-points (D E), PECs (B D) and MLN cells (C E), cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3, CD4, CD8 and γδTCR, and intracellular IL-17, and analysed by flow cytometry. IL-1RI −/− and WT mice were infected with S. aureus (5×10 8 CFU) via i.p. injection and at 3 h post-infection PECs, cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3 and γδTCR, and intracellular IL-17, and analysed by flow cytometry (F). Results expressed as mean ± SEM of n=10 mice/ group, with representative FACS plots. *

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Staphylococcus aureus infection of mice expands a population of memory γδ T cells that are protective against subsequent infection

    doi: 10.4049/jimmunol.1303420

    Figure Lengend Snippet: γδ T cells are the primary source of IL-17 during S. aureus induced peritonitis Mice were infected with S. aureus (5×10 8 CFU) via i.p. injection. At the indicated times post–infection, the peritoneal cavity was lavaged and the MLN collected. Secreted IL-17A and IL-1β in the peritoneal fluid was measured by ELISA (A). 3 h post-infection (B, C), and at the indicated time-points (D E), PECs (B D) and MLN cells (C E), cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3, CD4, CD8 and γδTCR, and intracellular IL-17, and analysed by flow cytometry. IL-1RI −/− and WT mice were infected with S. aureus (5×10 8 CFU) via i.p. injection and at 3 h post-infection PECs, cultured with Brefeldin A but not PMA and ionomycin, were stained for surface CD3 and γδTCR, and intracellular IL-17, and analysed by flow cytometry (F). Results expressed as mean ± SEM of n=10 mice/ group, with representative FACS plots. *

    Article Snippet: Cells were fixed and permeabilised using the Dako cytomation Intrastain Kit, before intracellular staining with a fluorochrome-conjugated antibodies against IL-17A (eBioscience, clone 17B7) and IFNγ (eBioscience, clone XMG1.2).

    Techniques: Mouse Assay, Infection, Injection, Enzyme-linked Immunosorbent Assay, Cell Culture, Staining, Flow Cytometry, Cytometry, FACS

    Human S . aureus bloodstream infection is associated with increased IFNγ production. Serum from S . aureus and E . coli bloodstream infection patients was collected on day 7 ± 2 post-initial bacteraemia and assessed for IFNγ (A) and IL-17A (B) by ELISA. Results expressed as individual patient values with median indicated by bar, n = 11–24 per group. *p

    Journal: PLoS Pathogens

    Article Title: Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection

    doi: 10.1371/journal.ppat.1005226

    Figure Lengend Snippet: Human S . aureus bloodstream infection is associated with increased IFNγ production. Serum from S . aureus and E . coli bloodstream infection patients was collected on day 7 ± 2 post-initial bacteraemia and assessed for IFNγ (A) and IL-17A (B) by ELISA. Results expressed as individual patient values with median indicated by bar, n = 11–24 per group. *p

    Article Snippet: Cells were fixed and permeabilised using the Dako IntraStain Fixation and Permeabilization Kit, before intracellular staining with fluorochrome-conjugated antibodies against IL-17A (eBioscience, clone 17B7) and IFNγ (eBioscience, clone XMG1.2).

    Techniques: Infection, Enzyme-linked Immunosorbent Assay

    Human S . aureus bloodstream infection induces S . aureus antigen-specific Th1 cells. PBMCs were isolated from patients, CFSE-labelled and incubated with heat-killed S . aureus PS80 strain (1μg/ml ≈ 1x10 7 CFU/ml) or media alone for 10 d before assessing S . aureus antigen-specific proliferation by gating on CFSE lo cells of the CD4 + population using flow cytometry (A). S . aureus antigen-specific Th1 and cytotoxic T cell division was assessed by gating on CFSE lo IFNγ + cells of the CD4 + and CD8 + populations respectively (B). S . aureus antigen-specific Th1 and Th17 proportions were compared by gating on CFSE lo IFNγ + or IL-17A + cells in the CD4 + population (C). For each patient, media only responses were subtracted from responses to heat-killed S . aureus to determine the antigen-specific response. Results shown as box-and-whiskers plots where the horizontal line indicates the median, boundaries of the box indicate the IQR, and whiskers indicate the highest and lowest values of the results, and representative FACS plots of CD4 + or CD8 + lymphocytes (A, B). n = 5–17 per group. SA = S . aureus , EC = E . coli , BSI = bloodstream infection. *p

    Journal: PLoS Pathogens

    Article Title: Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection

    doi: 10.1371/journal.ppat.1005226

    Figure Lengend Snippet: Human S . aureus bloodstream infection induces S . aureus antigen-specific Th1 cells. PBMCs were isolated from patients, CFSE-labelled and incubated with heat-killed S . aureus PS80 strain (1μg/ml ≈ 1x10 7 CFU/ml) or media alone for 10 d before assessing S . aureus antigen-specific proliferation by gating on CFSE lo cells of the CD4 + population using flow cytometry (A). S . aureus antigen-specific Th1 and cytotoxic T cell division was assessed by gating on CFSE lo IFNγ + cells of the CD4 + and CD8 + populations respectively (B). S . aureus antigen-specific Th1 and Th17 proportions were compared by gating on CFSE lo IFNγ + or IL-17A + cells in the CD4 + population (C). For each patient, media only responses were subtracted from responses to heat-killed S . aureus to determine the antigen-specific response. Results shown as box-and-whiskers plots where the horizontal line indicates the median, boundaries of the box indicate the IQR, and whiskers indicate the highest and lowest values of the results, and representative FACS plots of CD4 + or CD8 + lymphocytes (A, B). n = 5–17 per group. SA = S . aureus , EC = E . coli , BSI = bloodstream infection. *p

    Article Snippet: Cells were fixed and permeabilised using the Dako IntraStain Fixation and Permeabilization Kit, before intracellular staining with fluorochrome-conjugated antibodies against IL-17A (eBioscience, clone 17B7) and IFNγ (eBioscience, clone XMG1.2).

    Techniques: Infection, Isolation, Incubation, Flow Cytometry, Cytometry, FACS