fluorexon calcein  (Millipore)


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  • 99
    Name:
    Calcein
    Description:
    Calcein is useful in fitting soft and hybrid contact lenses along with its evaluation It has a molecular weight of 710kDa and is generally preferred over fluorescein Calcein is known to stain devitalized tissue It is not preferred for highly hydrated soft lenses
    Catalog Number:
    c0875
    Price:
    None
    Applications:
    Calcein has been used:. in whole cell staining . in histomorphometry and micro-computed tomography. to label active bone-forming surfaces
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    Structured Review

    Millipore fluorexon calcein
    Calcein
    Calcein is useful in fitting soft and hybrid contact lenses along with its evaluation It has a molecular weight of 710kDa and is generally preferred over fluorescein Calcein is known to stain devitalized tissue It is not preferred for highly hydrated soft lenses
    https://www.bioz.com/result/fluorexon calcein/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fluorexon calcein - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Large anti-HER2/neu liposomes for potential targeted intraperitoneal therapy of micrometastatic cancer"

    Article Title: Large anti-HER2/neu liposomes for potential targeted intraperitoneal therapy of micrometastatic cancer

    Journal: Journal of liposome research

    doi: 10.3109/08982100903544185

    Fluorescence imaging of intact large PEGylated liposomes in the peritoneal cavity. Detection of intact liposomes in the peritoneum came from using fluorescence imaging of i.p.-administered calcein-containing zwitterionic liposomes. Mice were sacrificed
    Figure Legend Snippet: Fluorescence imaging of intact large PEGylated liposomes in the peritoneal cavity. Detection of intact liposomes in the peritoneum came from using fluorescence imaging of i.p.-administered calcein-containing zwitterionic liposomes. Mice were sacrificed

    Techniques Used: Fluorescence, Imaging, Mouse Assay

    Related Articles

    Chromatography:

    Article Title: Transferrin-conjugated lipid-coated PLGA nanoparticles for targeted delivery of aromatase inhibitor 7?-APTADD to breast cancer cells
    Article Snippet: .. Poly(d, l-lactide-coglycolide), holo-human Tf, triethylamine, bovine serum albumin, sepharose CL-4B chromatography media, calcein, androst-4-ene-3,17-dione, bisbenzimide (Hoechst 33258), deoxyribonucleic acid (DNA), 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS), phenazine methosulfate (PMS) and solvents were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. DRAQ 5 was purchased from Biostatus Limited Inc. (Leicestershire, UK).

    Mouse Assay:

    Article Title: Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism
    Article Snippet: .. Briefly, the mice were intraperitoneally injected with 10 mg/kg body weight calcein (Sigma) dissolved in PBS at 10 and 3 days before sacrifice. ..

    Concentration Assay:

    Article Title: Human β-defensin 2 kills Candida albicans through phosphatidylinositol 4,5-bisphosphate–mediated membrane permeabilization
    Article Snippet: .. Calcein-encapsulated liposome assay The lipid films were rehydrated to a final concentration of 10 mg/ml in 100 mM calcein (Sigma-Aldrich), 20 mM Hepes (pH 7.4), and 1 mM EDTA at 60°C for 2 hours. .. Following three freeze-thaw cycles (alternating between liquid nitrogen and 60°C water bath), unilamellar liposomes were extruded 15 to 20 times through a mini-extruder with a 100-μm membrane (Avanti Polar Lipids).

    Incubation:

    Article Title: Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds
    Article Snippet: .. For fluorescent staining of bones, embryos were fixed in 4% of paraformaldehyde solution, washed and incubated for 2 hours in 10 μl/ml of Calcein (Sigma-Aldrich), and then cleared with Urea 4 M. Paraffin sections were cut at 7 μm thick and stained with Safranin and Hematoxylin employing standard histological protocols. .. (c) Whole Mount Immunofluorescence Embryos were fixed in Dent’s Fix (4:1 methanol:DMSO) for 2 hours at RT, dehydrated in 100% methanol and left at −80 °C overnight.

    Staining:

    Article Title: Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds
    Article Snippet: .. For fluorescent staining of bones, embryos were fixed in 4% of paraformaldehyde solution, washed and incubated for 2 hours in 10 μl/ml of Calcein (Sigma-Aldrich), and then cleared with Urea 4 M. Paraffin sections were cut at 7 μm thick and stained with Safranin and Hematoxylin employing standard histological protocols. .. (c) Whole Mount Immunofluorescence Embryos were fixed in Dent’s Fix (4:1 methanol:DMSO) for 2 hours at RT, dehydrated in 100% methanol and left at −80 °C overnight.

    Injection:

    Article Title: Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism
    Article Snippet: .. Briefly, the mice were intraperitoneally injected with 10 mg/kg body weight calcein (Sigma) dissolved in PBS at 10 and 3 days before sacrifice. ..

    Article Title: In-vivo performance of plasma-sprayed CaO–MgO–SiO2-based bioactive glass-ceramic coating on Ti–6Al–4V alloy for bone regeneration
    Article Snippet: .. For 3-month implantation, tetracycline (20 mg/kg, Sigma) used as the first label was performed at 13 and 14 days before sacrificing the rabbits by an subcutaneous injection, and calcein (10 mg/kg, Sigma) as the second label was injected 3 and 4 days before sacrificing. ..

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  • 99
    Millipore calcein
    A.) <t>Calcein</t> AM imaging demonstrating cell viability between vehicle control and BSO treatment groups at 24, 36 and 48 hours. Scale bar = 200 µm. B.) Effects of E2, PPT, DPN, ZYC-26 and ZYC-23 on cell viability in BSO-treated FRDA fibroblasts. All steroid concentrations were 100 nM, DMSO concentration was 0.1% and BSO concentration was 1 mM. Depicted are mean ± SD for n = 8 per group. * indicated p
    Calcein, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein/product/Millipore
    Average 99 stars, based on 330 article reviews
    Price from $9.99 to $1999.99
    calcein - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore calcein am
    Tendons have a cell-retentive barrier. A. Ex vivo mouse flexor tendon embedded in a fibrin gel. B. Ex vivo tendons were briefly subjected to enzymatic treatment (trypsin) and imaged 5 days later. Cells were labeled with <t>Calcein</t> AM (green, living cells) and propidium iodide (red, dead cells).
    Calcein Am, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein am/product/Millipore
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    calcein am - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    A.) Calcein AM imaging demonstrating cell viability between vehicle control and BSO treatment groups at 24, 36 and 48 hours. Scale bar = 200 µm. B.) Effects of E2, PPT, DPN, ZYC-26 and ZYC-23 on cell viability in BSO-treated FRDA fibroblasts. All steroid concentrations were 100 nM, DMSO concentration was 0.1% and BSO concentration was 1 mM. Depicted are mean ± SD for n = 8 per group. * indicated p

    Journal: PLoS ONE

    Article Title: Estrogen Prevents Oxidative Damage to the Mitochondria in Friedreich's Ataxia Skin Fibroblasts

    doi: 10.1371/journal.pone.0034600

    Figure Lengend Snippet: A.) Calcein AM imaging demonstrating cell viability between vehicle control and BSO treatment groups at 24, 36 and 48 hours. Scale bar = 200 µm. B.) Effects of E2, PPT, DPN, ZYC-26 and ZYC-23 on cell viability in BSO-treated FRDA fibroblasts. All steroid concentrations were 100 nM, DMSO concentration was 0.1% and BSO concentration was 1 mM. Depicted are mean ± SD for n = 8 per group. * indicated p

    Article Snippet: After 24, 36 and 48 hours of BSO treatment, the media was removed, and 1 µg/mL Calcein AM (CalBiochem, San Diego, CA, USA) in phosphate buffer pH 7.2 (PBS; Fisher Scientific, Pittsburg, PA, USA) was added to each well and the plate was incubated for 10 minutes at 37°C.

    Techniques: Imaging, Concentration Assay

    Histological evaluation of DBM regenerated bone tissue at 12 weeks after accurate and proactive immunomodulation via four different doses of IL-4 (0 ng, 10 ng, 50 ng and 100 ng). (A) H E and (B) Masson's trichrome staining further confirmed the micro-CT findings that the 0 ng group exhibited a bone tissue in-growth pattern from peripheral region, while the 10 ng and 50 ng groups showed a bone tissue in-growth pattern from the peripheral region and an in-situ bone island formation pattern in the central region. However, the 100 ng group exhibited non-union healing, and the defects were filled with loose connective fibrous tissues surrounding the minimal new bone formation. Bar = 500 μm. (C) Fluorescent images of newly formed bone double-labeled with calcein (green) and alizarin red (red). Bar = 100 μm. (D) Summary of fluorescence labeling. (E) Van Gieson staining for new bone formation (red particles). Bar = 500 μm. (F) Summary of Van Gieson staining results. For all charts, the groups designated by different uppercase letters or lowercase letters were significantly different (p

    Journal: Theranostics

    Article Title: Development of an Accurate and Proactive Immunomodulatory Strategy to Improve Bone Substitute Material-Mediated Osteogenesis and Angiogenesis

    doi: 10.7150/thno.28315

    Figure Lengend Snippet: Histological evaluation of DBM regenerated bone tissue at 12 weeks after accurate and proactive immunomodulation via four different doses of IL-4 (0 ng, 10 ng, 50 ng and 100 ng). (A) H E and (B) Masson's trichrome staining further confirmed the micro-CT findings that the 0 ng group exhibited a bone tissue in-growth pattern from peripheral region, while the 10 ng and 50 ng groups showed a bone tissue in-growth pattern from the peripheral region and an in-situ bone island formation pattern in the central region. However, the 100 ng group exhibited non-union healing, and the defects were filled with loose connective fibrous tissues surrounding the minimal new bone formation. Bar = 500 μm. (C) Fluorescent images of newly formed bone double-labeled with calcein (green) and alizarin red (red). Bar = 100 μm. (D) Summary of fluorescence labeling. (E) Van Gieson staining for new bone formation (red particles). Bar = 500 μm. (F) Summary of Van Gieson staining results. For all charts, the groups designated by different uppercase letters or lowercase letters were significantly different (p

    Article Snippet: Double fluorescence labeling and Van Gieson staining To further evaluate the rate of new bone formation, 4 rats from each group were injected with alizarin red (30 mg/kg; Millipore Sigma) and calcein (20 mg/kg; Millipore Sigma) at 6 and 9 weeks, respectively.

    Techniques: Staining, Micro-CT, In Situ, Labeling, Fluorescence

    VCAM-1 is involved in PCB118-induced adhesion of tumor cells to brain endothelial cells in vitro . Confluent mouse brain endothelial cells (bEND.3 cell line) were exposed to PCB118 (10μM) or vehicle for 18 h, followed by incubation with anti-ICAM-1– (30μM) or anti-VCAM-1–neutralizing (10μM) antibody (Ab) for 1 h. Then, calcein-labeled D122 cells were added to endothelial cultures for additional 90 min. Adhesion of D122 cells was evaluated by fluorescence measurement. Data are mean ± SD from three independent experiments, n = 6 cultures per group. *Significantly different as compared with controls at p

    Journal: Toxicological Sciences

    Article Title: Proinflammatory Adhesion Molecules Facilitate Polychlorinated Biphenyl-Mediated Enhancement of Brain Metastasis Formation

    doi: 10.1093/toxsci/kfr349

    Figure Lengend Snippet: VCAM-1 is involved in PCB118-induced adhesion of tumor cells to brain endothelial cells in vitro . Confluent mouse brain endothelial cells (bEND.3 cell line) were exposed to PCB118 (10μM) or vehicle for 18 h, followed by incubation with anti-ICAM-1– (30μM) or anti-VCAM-1–neutralizing (10μM) antibody (Ab) for 1 h. Then, calcein-labeled D122 cells were added to endothelial cultures for additional 90 min. Adhesion of D122 cells was evaluated by fluorescence measurement. Data are mean ± SD from three independent experiments, n = 6 cultures per group. *Significantly different as compared with controls at p

    Article Snippet: bEND.3 cells (mouse brain endothelial cell line) were grown to confluence on 24-well plates and exposed to PCB118 (10μM) or vehicle for 18 h. Then, cultures were incubated with anti-ICAM-1- or anti-VCAM-1-neutralizing antibody (both from BioLegends, San Diego, CA) for 1 h. D122-Luc/GFP cells were labeled with calcein AM (Calbiochem, La Jolla, CA), as described earlier , and added in the amount of 4 × 105 cells/ml onto endothelial monolayers.

    Techniques: In Vitro, Incubation, Labeling, Fluorescence

    Tendons have a cell-retentive barrier. A. Ex vivo mouse flexor tendon embedded in a fibrin gel. B. Ex vivo tendons were briefly subjected to enzymatic treatment (trypsin) and imaged 5 days later. Cells were labeled with Calcein AM (green, living cells) and propidium iodide (red, dead cells).

    Journal: PLoS ONE

    Article Title: Tendon Is Covered by a Basement Membrane Epithelium That Is Required for Cell Retention and the Prevention of Adhesion Formation

    doi: 10.1371/journal.pone.0016337

    Figure Lengend Snippet: Tendons have a cell-retentive barrier. A. Ex vivo mouse flexor tendon embedded in a fibrin gel. B. Ex vivo tendons were briefly subjected to enzymatic treatment (trypsin) and imaged 5 days later. Cells were labeled with Calcein AM (green, living cells) and propidium iodide (red, dead cells).

    Article Snippet: Calcein AM (Calbiochem, NJ, USA) was prepared as 10 mM solution in 0.1% v/v DMSO.

    Techniques: Ex Vivo, Labeling