fluorescent 5 nuclease real time polymerase chain reaction  (Thermo Fisher)


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    Thermo Fisher fluorescent 5 nuclease real time polymerase chain reaction
    Fluorescent 5 Nuclease Real Time Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent 5 nuclease real time polymerase chain reaction/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent 5 nuclease real time polymerase chain reaction - by Bioz Stars, 2020-07
    85/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Decreased PM10 Exposure Attenuates Age-Related Lung Function Decline: Genetic Variants in p53, p21, and CCND1 Modify This Effect
    Article Snippet: .. Genotyping strategy used was fluorescent 5′-nuclease real-time polymerase chain reaction (TaqMan, Applera Europe, Rotkreuz, Switzerland) methodology using ABI Prism 7900 sequence detection system (ABI, Rotkreuz, Switzerland). .. The SNP-specific primers and locked nucleic acid (LNA) dual-labeled fluorogenic probes were designed by Sigma Proligo (Evry, France).

    Sequencing:

    Article Title: Decreased PM10 Exposure Attenuates Age-Related Lung Function Decline: Genetic Variants in p53, p21, and CCND1 Modify This Effect
    Article Snippet: .. Genotyping strategy used was fluorescent 5′-nuclease real-time polymerase chain reaction (TaqMan, Applera Europe, Rotkreuz, Switzerland) methodology using ABI Prism 7900 sequence detection system (ABI, Rotkreuz, Switzerland). .. The SNP-specific primers and locked nucleic acid (LNA) dual-labeled fluorogenic probes were designed by Sigma Proligo (Evry, France).

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    Thermo Fisher hela cells nuclease free microcentrifuge tubes rnase r
    Depletion of linear RNA by <t>RNase</t> R digestion. RT-qPCR analysis of subsets of linear rRNA, mRNAs and circRNAs (A), as well as snRNAs and 5S rRNA (B) in <t>HeLa</t> cell RNA samples left untreated or treated with RNase R. In (A) since the circRNA levels were not affected by RNase R treatment, mRNAs left after RNase R treatment were normalized to the levels of the corresponding circRNAs. Data represent the means ± SEM from 4 or more experiments.
    Hela Cells Nuclease Free Microcentrifuge Tubes Rnase R, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hela cells nuclease free microcentrifuge tubes rnase r/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hela cells nuclease free microcentrifuge tubes rnase r - by Bioz Stars, 2020-07
    99/100 stars
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    Depletion of linear RNA by RNase R digestion. RT-qPCR analysis of subsets of linear rRNA, mRNAs and circRNAs (A), as well as snRNAs and 5S rRNA (B) in HeLa cell RNA samples left untreated or treated with RNase R. In (A) since the circRNA levels were not affected by RNase R treatment, mRNAs left after RNase R treatment were normalized to the levels of the corresponding circRNAs. Data represent the means ± SEM from 4 or more experiments.

    Journal: Methods (San Diego, Calif.)

    Article Title: RPAD (RNase R Treatment, Polyadenylation, and Poly(A)+ RNA Depletion) Method to Isolate Highly Pure Circular RNA

    doi: 10.1016/j.ymeth.2018.10.022

    Figure Lengend Snippet: Depletion of linear RNA by RNase R digestion. RT-qPCR analysis of subsets of linear rRNA, mRNAs and circRNAs (A), as well as snRNAs and 5S rRNA (B) in HeLa cell RNA samples left untreated or treated with RNase R. In (A) since the circRNA levels were not affected by RNase R treatment, mRNAs left after RNase R treatment were normalized to the levels of the corresponding circRNAs. Data represent the means ± SEM from 4 or more experiments.

    Article Snippet: Total RNA isolated from HeLa cells Nuclease-free microcentrifuge tubes RNase R (Lucigen, Epicentre) TURBO DNA-free™ Kit (Thermo Fisher Scientific) RiboLock RNase inhibitor (Thermo Fisher Scientific) Thermomixer (Eppendorf) miRNeasy Kit (QIAGEN) Vortexer Microcentrifuge (Eppendorf) NanoDrop spectrophotometer (Thermo Fisher Scientific)

    Techniques: Quantitative RT-PCR