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Hamamatsu fluorescence signals
Fluorescence Signals, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 94/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 75 article reviews
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fluorescence signals - by Bioz Stars, 2020-04
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Synthesized:

Article Title: Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis
Article Snippet: To induce activation of sperm, dye-loaded sperm were diluted 1,000-fold with ASW containing 100 nM SAAF, which was synthesized as described previously ( , ). .. The intensity of the fluorescence signals was analyzed by Aquacosmos (Hamamatsu Photonics).

Blocking Assay:

Article Title: Cerium oxide nanoparticles inhibit differentiation of neural stem cells
Article Snippet: .. Fluorescence signals were passed through a 0.9–1.0 Airy unit pinhole, a tunable AOBS spectral excitation/filtering unit and separate notch (blocking) filters placed in front of integrated hybrid detectors (APD/PMT modules from Hamamatsu Photonics). ..

Incubation:

Article Title: UDP induces intestinal epithelial migration via the P2Y6 receptor
Article Snippet: In brief, IEC-6 cells cultured on glass coverslips were incubated with 3 μmol·L−1 fura-2 AM containing 0.01% Cremophor in HEPES solution in the dark at 37°C for 40 min. .. The fluorescence ratio (R: F340/F380) was determined by the fluorescence signals collected every 3 s at 340 nm (F340) and 380 nm (F380) using a fluorescence imaging system (Hamamatsu Photonics, Hamamatsu, Japan).

Article Title: Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis
Article Snippet: Briefly, sperm were suspended in five volumes of low-Ca2+ ASW (pH 7.2), containing 460 mM NaCl, 10 mM KCl, 1 mM CaCl2 , 36 mM MgCl2 , 17.5 mM MgSO4 , 0.1 mM EDTA, and 10 mM Hepes-NaOH (pH 7.2), containing 0.05% Cremophor EL (Nacalai Tesque) and 20 μM Fluo-8H-AM (Molecular Probes), and were then incubated for 2 h at 18 °C. .. The intensity of the fluorescence signals was analyzed by Aquacosmos (Hamamatsu Photonics).

Diffusion-based Assay:

Article Title: A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Article Snippet: Calcium imaging was started at least 30 min after break-in to allow diffusion of the dye into the cell. .. The fluorescence signals of Cal-520 or OGB-1 and Alexa 594 were separated into green and red channels, respectively, and detected by a pair of photomultiplier tubes (Hamamatsu).

Mass Spectrometry:

Article Title: A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Article Snippet: The fluorescence signals of Cal-520 or OGB-1 and Alexa 594 were separated into green and red channels, respectively, and detected by a pair of photomultiplier tubes (Hamamatsu). .. APs were triggered by current injections (0.8 nA, 2 ms) through the patch pipette.

Article Title: Axial Tubule Junctions Activate Atrial Ca2+ Release Across Species
Article Snippet: Fluorescence signals are detected by two photomultiplier tubes (H7422, Hamamatsu) using an emission filter of 655 ± 20 nm. .. Square pulses of 10–20 V and duration of 3 ms were used to reach action potential threshold.

Immunohistochemistry:

Article Title: Brain Circuits Mediating Opposing Effects on Emotion and Pain
Article Snippet: Paragraph title: Immunohistochemistry. ... Intensity of fluorescence signals for c-fos staining in images was automatically quantified and analyzed by HCImage software (Hamamatsu).

Activation Assay:

Article Title: Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis
Article Snippet: To induce activation of sperm, dye-loaded sperm were diluted 1,000-fold with ASW containing 100 nM SAAF, which was synthesized as described previously ( , ). .. The intensity of the fluorescence signals was analyzed by Aquacosmos (Hamamatsu Photonics).

Transferring:

Article Title: A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Article Snippet: The resistance of the patch pipette ranged from 4 to 8 MΩ when filled with the intracellular solution containing (in mm ): 133 K-MeSO3 , 7.4 KCl, 10 HEPES, 3 Na2 ATP, 0.3 Na2 GTP, 0.3 MgCl2 , 0.05 Alexa 594, and either 0.1 Cal-520 potassium salt (AAT Bioquest) or 0.1 OGB-1 potassium salt (Invitrogen). .. The fluorescence signals of Cal-520 or OGB-1 and Alexa 594 were separated into green and red channels, respectively, and detected by a pair of photomultiplier tubes (Hamamatsu).

Cell Culture:

Article Title: Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis
Article Snippet: Cells were cultured on coverslips and loaded with 5 μM Cu2 (FL2E) NO dye ( ) for 1 h in culture medium at 37 °C and 2% CO2 . .. Fluorescence signals were analyzed by using a Hamamatsu Orca CCD camera (Hamamatsu) coupled to an inverted microscope (IX81; Olympus) at 470 nm.

Article Title: UDP induces intestinal epithelial migration via the P2Y6 receptor
Article Snippet: In brief, IEC-6 cells cultured on glass coverslips were incubated with 3 μmol·L−1 fura-2 AM containing 0.01% Cremophor in HEPES solution in the dark at 37°C for 40 min. .. The fluorescence ratio (R: F340/F380) was determined by the fluorescence signals collected every 3 s at 340 nm (F340) and 380 nm (F380) using a fluorescence imaging system (Hamamatsu Photonics, Hamamatsu, Japan).

Generated:

Article Title: Cerium oxide nanoparticles inhibit differentiation of neural stem cells
Article Snippet: To confirm the SIM generated images, super-resolution STED imaging was applied, using a Leica SP8 (3X) STED system equipped with a white light source for excitation (tunable excitation from 470–670 nm) and three STED lasers for depletion at 592 nm, 660 nm and 775 nm. .. Fluorescence signals were passed through a 0.9–1.0 Airy unit pinhole, a tunable AOBS spectral excitation/filtering unit and separate notch (blocking) filters placed in front of integrated hybrid detectors (APD/PMT modules from Hamamatsu Photonics).

Article Title: Imaging and Tracking of Bone Marrow-Derived Immune and Stem Cells
Article Snippet: OCT/OCM images are generated after several processing steps including compensating for unbalanced dispersion in the sample arm and for nonuniform distribution of the spectrum on the CCD due to nonlinearity of the diffraction grating. .. The fluorescence signals of different spectral band are detected separately by two photomultiplier tubes (H7421, Hamamatsu).

Imaging:

Article Title: Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis
Article Snippet: Paragraph title: Intracellular Nitric Oxide Imaging. ... Fluorescence signals were analyzed by using a Hamamatsu Orca CCD camera (Hamamatsu) coupled to an inverted microscope (IX81; Olympus) at 470 nm.

Article Title: Cerium oxide nanoparticles inhibit differentiation of neural stem cells
Article Snippet: Imaging of nuclear staining with DAPI was A 100X/1.4 NA oil immersion objective lens (HCX PL APO STED white, Leica Microsystems) was used for the imaging. .. Fluorescence signals were passed through a 0.9–1.0 Airy unit pinhole, a tunable AOBS spectral excitation/filtering unit and separate notch (blocking) filters placed in front of integrated hybrid detectors (APD/PMT modules from Hamamatsu Photonics).

Article Title: A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Article Snippet: Paragraph title: Simultaneous whole-cell recordings and calcium imaging in vitro ... The fluorescence signals of Cal-520 or OGB-1 and Alexa 594 were separated into green and red channels, respectively, and detected by a pair of photomultiplier tubes (Hamamatsu).

Article Title: UDP induces intestinal epithelial migration via the P2Y6 receptor
Article Snippet: .. The fluorescence ratio (R: F340/F380) was determined by the fluorescence signals collected every 3 s at 340 nm (F340) and 380 nm (F380) using a fluorescence imaging system (Hamamatsu Photonics, Hamamatsu, Japan). ..

Article Title: Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis
Article Snippet: Paragraph title: [Ca2+ ]i Fluorescence Imaging of Sperm. ... The intensity of the fluorescence signals was analyzed by Aquacosmos (Hamamatsu Photonics).

Article Title: N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve
Article Snippet: Paragraph title: Ca2+ Imaging with Two-Photon Excitation Microscopy ... The dye was excited at 790 nm (Spectra-Physics MaiTai HP Laser) and fluorescence signals were detected using a high sensitivity photomultiplier H7422-40 (Hamamatsu, Japan), after filtering with a DCLP dichroic mirror > 500 nm.

Article Title: Axial Tubule Junctions Activate Atrial Ca2+ Release Across Species
Article Snippet: The RAMP imaging system has been described previously in detail ( ; , ). .. Fluorescence signals are detected by two photomultiplier tubes (H7422, Hamamatsu) using an emission filter of 655 ± 20 nm.

Article Title: Effect of resveratrol treatment on graft revascularization after islet transplantation in streptozotocin-induced diabetic mice
Article Snippet: An excitation laser beam raster scanning pattern was produced by a laser scanner consisting of a rotating polygonal mirror (MC-5, Lincoln Laser) and a galvanometer-based scanning mirror (6230H, Cambridge Technology), and then transferred to the back aperture of an imaging objective lens. .. Fluorescence signals from the kidney were epi-detected by the objective lens and delivered to the photomultiplier tubes (PMT; R9110, Hamamatsu, Japan) through bandpass filters (BPF1; FF02-525/50, BPF2; FF01–600/37, BPF3; FF01–685/40, Semrock, Rochester, NY).

Inverted Microscopy:

Article Title: Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis
Article Snippet: .. Fluorescence signals were analyzed by using a Hamamatsu Orca CCD camera (Hamamatsu) coupled to an inverted microscope (IX81; Olympus) at 470 nm. .. Cardiac myocytes plated on glass bottom dishes (Mattek) were immersed in 4% paraformaldehyde for 20 min, rinsed twice with PBS, permeabilized in 0.1% Triton X-100 for 45 min, and blocked with 10% goat serum overnight.

Article Title: UDP induces intestinal epithelial migration via the P2Y6 receptor
Article Snippet: The fluorescence ratio (R: F340/F380) was determined by the fluorescence signals collected every 3 s at 340 nm (F340) and 380 nm (F380) using a fluorescence imaging system (Hamamatsu Photonics, Hamamatsu, Japan). .. The fluorescence ratio (R: F340/F380) was determined by the fluorescence signals collected every 3 s at 340 nm (F340) and 380 nm (F380) using a fluorescence imaging system (Hamamatsu Photonics, Hamamatsu, Japan).

In Vivo Imaging:

Article Title: Effect of resveratrol treatment on graft revascularization after islet transplantation in streptozotocin-induced diabetic mice
Article Snippet: Paragraph title: In vivo imaging system ... Fluorescence signals from the kidney were epi-detected by the objective lens and delivered to the photomultiplier tubes (PMT; R9110, Hamamatsu, Japan) through bandpass filters (BPF1; FF02-525/50, BPF2; FF01–600/37, BPF3; FF01–685/40, Semrock, Rochester, NY).

Immunofluorescence:

Article Title: Brain Circuits Mediating Opposing Effects on Emotion and Pain
Article Snippet: Intensity of fluorescence signals for c-fos staining in images was automatically quantified and analyzed by HCImage software (Hamamatsu). .. Cells with immunofluorescence intensity > 2-fold that of background immunofluorescence intensity were considered as c-fos-positive cells.

In Vivo:

Article Title: Effect of resveratrol treatment on graft revascularization after islet transplantation in streptozotocin-induced diabetic mice
Article Snippet: To visualize and quantify the angiogenesis in the transplanted islet in the kidney in vivo , a custom-built laser-scanning confocal microscopy system for intravital imaging was utilized., As previously described, continuous-wave laser modules at 488 nm (MLD488, Cobolt), 561 nm (Jive, Cobolt), and 640 nm (MLD640, Cobolt) were used as excitation sources for multi-color fluorescence imaging. .. Fluorescence signals from the kidney were epi-detected by the objective lens and delivered to the photomultiplier tubes (PMT; R9110, Hamamatsu, Japan) through bandpass filters (BPF1; FF02-525/50, BPF2; FF01–600/37, BPF3; FF01–685/40, Semrock, Rochester, NY).

Article Title: Calcium Channel Types with Distinct Presynaptic Localization Couple Differentially to Transmitter Release in Single Calyx-Type Synapses
Article Snippet: Calibration parameters were obtained from in vivo calibrations as described in . .. Fluorescence signals recorded by the photodiode (Hamamatsu) were filtered at 30 Hz (8-pole Bessel filter).

Fluorescence:

Article Title: Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis
Article Snippet: .. Fluorescence signals were analyzed by using a Hamamatsu Orca CCD camera (Hamamatsu) coupled to an inverted microscope (IX81; Olympus) at 470 nm. .. Cardiac myocytes plated on glass bottom dishes (Mattek) were immersed in 4% paraformaldehyde for 20 min, rinsed twice with PBS, permeabilized in 0.1% Triton X-100 for 45 min, and blocked with 10% goat serum overnight.

Article Title: Cerium oxide nanoparticles inhibit differentiation of neural stem cells
Article Snippet: .. Fluorescence signals were passed through a 0.9–1.0 Airy unit pinhole, a tunable AOBS spectral excitation/filtering unit and separate notch (blocking) filters placed in front of integrated hybrid detectors (APD/PMT modules from Hamamatsu Photonics). ..

Article Title: A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Article Snippet: .. The fluorescence signals of Cal-520 or OGB-1 and Alexa 594 were separated into green and red channels, respectively, and detected by a pair of photomultiplier tubes (Hamamatsu). .. Simultaneous whole-cell recording and linescan calcium imaging (714 Hz) were conducted on the soma of the pyramidal cells.

Article Title: UDP induces intestinal epithelial migration via the P2Y6 receptor
Article Snippet: .. The fluorescence ratio (R: F340/F380) was determined by the fluorescence signals collected every 3 s at 340 nm (F340) and 380 nm (F380) using a fluorescence imaging system (Hamamatsu Photonics, Hamamatsu, Japan). ..

Article Title: Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis
Article Snippet: .. The intensity of the fluorescence signals was analyzed by Aquacosmos (Hamamatsu Photonics). ..

Article Title: Imaging and Tracking of Bone Marrow-Derived Immune and Stem Cells
Article Snippet: .. The fluorescence signals of different spectral band are detected separately by two photomultiplier tubes (H7421, Hamamatsu). ..

Article Title: N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve
Article Snippet: .. The dye was excited at 790 nm (Spectra-Physics MaiTai HP Laser) and fluorescence signals were detected using a high sensitivity photomultiplier H7422-40 (Hamamatsu, Japan), after filtering with a DCLP dichroic mirror > 500 nm. .. A laser-scanning system (TriM Scope II, LaVisionBiotec, Germany) coupled to an upright microscope (Olympus, Japan) equipped with a 20×, NA 1.1 water-immersion objective (Zeiss, Germany) was controlled using ImSpector Pro Software (version 4.0, LaVision Biotec, Germany), which also allowed online analysis of the data.

Article Title: Axial Tubule Junctions Activate Atrial Ca2+ Release Across Species
Article Snippet: .. Fluorescence signals are detected by two photomultiplier tubes (H7422, Hamamatsu) using an emission filter of 655 ± 20 nm. ..

Article Title: Effect of resveratrol treatment on graft revascularization after islet transplantation in streptozotocin-induced diabetic mice
Article Snippet: .. Fluorescence signals from the kidney were epi-detected by the objective lens and delivered to the photomultiplier tubes (PMT; R9110, Hamamatsu, Japan) through bandpass filters (BPF1; FF02-525/50, BPF2; FF01–600/37, BPF3; FF01–685/40, Semrock, Rochester, NY). ..

Article Title: The Spread of Ras Activity Triggered by Activation of a Single Dendritic Spine
Article Snippet: .. Fluorescence signals from PMTs (R3896, Hamamatsu) were acquired by ScanImage using a data acquisition board (PCI-6110, National Instruments). ..

Article Title: Brain Circuits Mediating Opposing Effects on Emotion and Pain
Article Snippet: .. Intensity of fluorescence signals for c-fos staining in images was automatically quantified and analyzed by HCImage software (Hamamatsu). ..

Article Title: Calcium Channel Types with Distinct Presynaptic Localization Couple Differentially to Transmitter Release in Single Calyx-Type Synapses
Article Snippet: .. Fluorescence signals recorded by the photodiode (Hamamatsu) were filtered at 30 Hz (8-pole Bessel filter). ..

Isolation:

Article Title: N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve
Article Snippet: Single pulses (pulse length 200–500 μs, pulse amplitude 50 V) were applied every 30 s using isolated pulse stimulator (ISO-STIM 01D, NPI Electronic, Germany). .. The dye was excited at 790 nm (Spectra-Physics MaiTai HP Laser) and fluorescence signals were detected using a high sensitivity photomultiplier H7422-40 (Hamamatsu, Japan), after filtering with a DCLP dichroic mirror > 500 nm.

Microscopy:

Article Title: Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis
Article Snippet: Coverslips were then placed in an onstage incubator (Tokai) on the microscope in a low-volume glass-covered recording chamber. .. Fluorescence signals were analyzed by using a Hamamatsu Orca CCD camera (Hamamatsu) coupled to an inverted microscope (IX81; Olympus) at 470 nm.

Article Title: Cerium oxide nanoparticles inhibit differentiation of neural stem cells
Article Snippet: Paragraph title: Super-resolution microscopy ... Fluorescence signals were passed through a 0.9–1.0 Airy unit pinhole, a tunable AOBS spectral excitation/filtering unit and separate notch (blocking) filters placed in front of integrated hybrid detectors (APD/PMT modules from Hamamatsu Photonics).

Article Title: A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Article Snippet: A laser scanning two-photon microscope (FV1000-MPE; Olympus) equipped with a pulsed laser (MaiTai HP DeepSee; NewPort Spectra-Physics) was used for two-photon calcium imaging with a water-immersion objective lens (LUMPLFL/IR40XW, Olympus). .. The fluorescence signals of Cal-520 or OGB-1 and Alexa 594 were separated into green and red channels, respectively, and detected by a pair of photomultiplier tubes (Hamamatsu).

Article Title: Imaging and Tracking of Bone Marrow-Derived Immune and Stem Cells
Article Snippet: Paragraph title: 2.4 Integrated Microscope ... The fluorescence signals of different spectral band are detected separately by two photomultiplier tubes (H7421, Hamamatsu).

Article Title: N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve
Article Snippet: Paragraph title: Ca2+ Imaging with Two-Photon Excitation Microscopy ... The dye was excited at 790 nm (Spectra-Physics MaiTai HP Laser) and fluorescence signals were detected using a high sensitivity photomultiplier H7422-40 (Hamamatsu, Japan), after filtering with a DCLP dichroic mirror > 500 nm.

Article Title: Axial Tubule Junctions Activate Atrial Ca2+ Release Across Species
Article Snippet: Paragraph title: Random Access Multi-Photon Microscopy (RAMP) ... Fluorescence signals are detected by two photomultiplier tubes (H7422, Hamamatsu) using an emission filter of 655 ± 20 nm.

Article Title: Brain Circuits Mediating Opposing Effects on Emotion and Pain
Article Snippet: The stained sections were examined with an Olympus BX51 fluorescence microscope or a Zeiss 710 confocal microscope. .. Intensity of fluorescence signals for c-fos staining in images was automatically quantified and analyzed by HCImage software (Hamamatsu).

Article Title: Calcium Channel Types with Distinct Presynaptic Localization Couple Differentially to Transmitter Release in Single Calyx-Type Synapses
Article Snippet: Excitation light was coupled to the microscope via a light guide. .. Fluorescence signals recorded by the photodiode (Hamamatsu) were filtered at 30 Hz (8-pole Bessel filter).

Confocal Microscopy:

Article Title: Effect of resveratrol treatment on graft revascularization after islet transplantation in streptozotocin-induced diabetic mice
Article Snippet: To visualize and quantify the angiogenesis in the transplanted islet in the kidney in vivo , a custom-built laser-scanning confocal microscopy system for intravital imaging was utilized., As previously described, continuous-wave laser modules at 488 nm (MLD488, Cobolt), 561 nm (Jive, Cobolt), and 640 nm (MLD640, Cobolt) were used as excitation sources for multi-color fluorescence imaging. .. Fluorescence signals from the kidney were epi-detected by the objective lens and delivered to the photomultiplier tubes (PMT; R9110, Hamamatsu, Japan) through bandpass filters (BPF1; FF02-525/50, BPF2; FF01–600/37, BPF3; FF01–685/40, Semrock, Rochester, NY).

Software:

Article Title: N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve
Article Snippet: The dye was excited at 790 nm (Spectra-Physics MaiTai HP Laser) and fluorescence signals were detected using a high sensitivity photomultiplier H7422-40 (Hamamatsu, Japan), after filtering with a DCLP dichroic mirror > 500 nm. .. A laser-scanning system (TriM Scope II, LaVisionBiotec, Germany) coupled to an upright microscope (Olympus, Japan) equipped with a 20×, NA 1.1 water-immersion objective (Zeiss, Germany) was controlled using ImSpector Pro Software (version 4.0, LaVision Biotec, Germany), which also allowed online analysis of the data.

Article Title: The Spread of Ras Activity Triggered by Activation of a Single Dendritic Spine
Article Snippet: Custom software (FLIMImage) integrated into ScanImage ( 14 ) was used to acquire lifetime images. .. Fluorescence signals from PMTs (R3896, Hamamatsu) were acquired by ScanImage using a data acquisition board (PCI-6110, National Instruments).

Article Title: Brain Circuits Mediating Opposing Effects on Emotion and Pain
Article Snippet: .. Intensity of fluorescence signals for c-fos staining in images was automatically quantified and analyzed by HCImage software (Hamamatsu). ..

Selection:

Article Title: Cerium oxide nanoparticles inhibit differentiation of neural stem cells
Article Snippet: SIM processing was done with the included ZEN software 2012 (SP2), with selection of automatic settings for evaluation of the raw data (i.e . theoretical PSF, selection of noise filter setting, frequency weighting, baseline settings etc.) .. Fluorescence signals were passed through a 0.9–1.0 Airy unit pinhole, a tunable AOBS spectral excitation/filtering unit and separate notch (blocking) filters placed in front of integrated hybrid detectors (APD/PMT modules from Hamamatsu Photonics).

In Vitro:

Article Title: A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Article Snippet: Paragraph title: Simultaneous whole-cell recordings and calcium imaging in vitro ... The fluorescence signals of Cal-520 or OGB-1 and Alexa 594 were separated into green and red channels, respectively, and detected by a pair of photomultiplier tubes (Hamamatsu).

Laser-Scanning Microscopy:

Article Title: N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve
Article Snippet: Ca2+ Imaging with Two-Photon Excitation Microscopy Individual nerve slices filled with Ca2+ indicator were transferred to a recording chamber mounted on the stage of a 2-photon laser-scanning microscope (LaVision Biotech, Germany) and perfused with ACSF containing 2.5 mM Ca2+ . .. The dye was excited at 790 nm (Spectra-Physics MaiTai HP Laser) and fluorescence signals were detected using a high sensitivity photomultiplier H7422-40 (Hamamatsu, Japan), after filtering with a DCLP dichroic mirror > 500 nm.

Produced:

Article Title: Effect of resveratrol treatment on graft revascularization after islet transplantation in streptozotocin-induced diabetic mice
Article Snippet: An excitation laser beam raster scanning pattern was produced by a laser scanner consisting of a rotating polygonal mirror (MC-5, Lincoln Laser) and a galvanometer-based scanning mirror (6230H, Cambridge Technology), and then transferred to the back aperture of an imaging objective lens. .. Fluorescence signals from the kidney were epi-detected by the objective lens and delivered to the photomultiplier tubes (PMT; R9110, Hamamatsu, Japan) through bandpass filters (BPF1; FF02-525/50, BPF2; FF01–600/37, BPF3; FF01–685/40, Semrock, Rochester, NY).

Concentration Assay:

Article Title: Calcium Channel Types with Distinct Presynaptic Localization Couple Differentially to Transmitter Release in Single Calyx-Type Synapses
Article Snippet: Fura-2 measurements of Ca2+ concentration were made by forming ratios between two continuous recordings (100–500 msec) of fluorescence (after background subtraction) at two excitation wavelengths (357 and 380 nm) with an interval of ∼10 msec ( ). .. Fluorescence signals recorded by the photodiode (Hamamatsu) were filtered at 30 Hz (8-pole Bessel filter).

Staining:

Article Title: Cerium oxide nanoparticles inhibit differentiation of neural stem cells
Article Snippet: Imaging of nuclear staining with DAPI was A 100X/1.4 NA oil immersion objective lens (HCX PL APO STED white, Leica Microsystems) was used for the imaging. .. Fluorescence signals were passed through a 0.9–1.0 Airy unit pinhole, a tunable AOBS spectral excitation/filtering unit and separate notch (blocking) filters placed in front of integrated hybrid detectors (APD/PMT modules from Hamamatsu Photonics).

Article Title: Brain Circuits Mediating Opposing Effects on Emotion and Pain
Article Snippet: .. Intensity of fluorescence signals for c-fos staining in images was automatically quantified and analyzed by HCImage software (Hamamatsu). ..

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    Hamamatsu fura 2 fluorescent signals
    Roles of PTx-sensitive G-proteins and intracellular Ca 2+ in COA-Cl-induced ERK1/2 phosphorylation responses in HUVEC. (A) Results of immunoblot (IB) analyses in which HUVEC were treated with COA-Cl either in the presence or absence of pretreatment with PTx. HUVEC were treated with 50 ng mL −1 of PTx overnight, followed by 100 μ mol/L of COA-Cl for 15 min. They were then subjected to IB analyses for phospho- and total-ERK1/2, as described above. (B) BAPTA-AM, a chelator of intracellular calcium (20 μ mol/L for 30 min), was used instead of PTx. (A and B) Representative results of three independent experiments, which yielded equivalent data. (C and D) Results of intracellular Ca 2+ transients assays in HUVEC loaded with <t>fura-2-AM.</t> (C) Representative trace of 1 mmol/L COA-C1-induced intracellular Ca 2+ increase; some cells had been treated with various inhibitors as described above prior to COA-Cl treatment. (D) Summarizes the results derived from pooled records obtained from 8−28 cells in each group. Fura-2 fluorescent signals were recorded and analyzed as described in the main text. Changes in the fura-2 ratio that corresponded to peak increases in intracellular Ca 2+ concentrations are shown as ▵F/F 0 . * P
    Fura 2 Fluorescent Signals, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fura 2 fluorescent signals/product/Hamamatsu
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fura 2 fluorescent signals - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    93
    Hamamatsu fura 2 fluorescence signals
    Two types of stretch-evoked responses of normal DRG neurons. ( a ) Representative <t>Fura-2</t> Ca 2+ imaging after stretch stimulation (10 and 100 s) in isolated DRG neurons from TRPV2 flox/flox mice. Arrowheads , fast-decay type; arrows , slow-decay type. ( b ) Typical Ca 2+ response after stretch stimulus in two subsets of neurons. ( c , d ) Stretch threshold of fast- and slow-decay type neurons (fast, n = 23; slow n = 18): Cumulative distribution of the threshold ( c ), median and IQR ( d ) (*** P
    Fura 2 Fluorescence Signals, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fura 2 fluorescence signals/product/Hamamatsu
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    fura 2 fluorescence signals - by Bioz Stars, 2020-04
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      Buy from Supplier

    93
    Hamamatsu meos3 2 β spectrin fluorescence signals
    SCA5 <t>β-spectrin</t> dominantly mislocalizes the endogenous spectrin cytoskeleton. ( A , Left ) Confocal images showing the localization of endogenous α-spectrin in the somata and dendrites of class IV da neurons expressing wild-type (WT) or SCA5 β-spectrin together with the fluorescent membrane marker CD4tdtomato. α-Spectrin can also be seen in the somata and dendrites of neighboring sensory neurons not expressing β-spectrin transgenes. (Scale bar, 50 µm.) ( Right ) Quantitation of α-spectrin in cell bodies. P
    Meos3 2 β Spectrin Fluorescence Signals, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/meos3 2 β spectrin fluorescence signals/product/Hamamatsu
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Roles of PTx-sensitive G-proteins and intracellular Ca 2+ in COA-Cl-induced ERK1/2 phosphorylation responses in HUVEC. (A) Results of immunoblot (IB) analyses in which HUVEC were treated with COA-Cl either in the presence or absence of pretreatment with PTx. HUVEC were treated with 50 ng mL −1 of PTx overnight, followed by 100 μ mol/L of COA-Cl for 15 min. They were then subjected to IB analyses for phospho- and total-ERK1/2, as described above. (B) BAPTA-AM, a chelator of intracellular calcium (20 μ mol/L for 30 min), was used instead of PTx. (A and B) Representative results of three independent experiments, which yielded equivalent data. (C and D) Results of intracellular Ca 2+ transients assays in HUVEC loaded with fura-2-AM. (C) Representative trace of 1 mmol/L COA-C1-induced intracellular Ca 2+ increase; some cells had been treated with various inhibitors as described above prior to COA-Cl treatment. (D) Summarizes the results derived from pooled records obtained from 8−28 cells in each group. Fura-2 fluorescent signals were recorded and analyzed as described in the main text. Changes in the fura-2 ratio that corresponded to peak increases in intracellular Ca 2+ concentrations are shown as ▵F/F 0 . * P

    Journal: Pharmacology Research & Perspectives

    Article Title: Involvement of S1P1 receptor pathway in angiogenic effects of a novel adenosine-like nucleic acid analog COA-Cl in cultured human vascular endothelial cells

    doi: 10.1002/prp2.68

    Figure Lengend Snippet: Roles of PTx-sensitive G-proteins and intracellular Ca 2+ in COA-Cl-induced ERK1/2 phosphorylation responses in HUVEC. (A) Results of immunoblot (IB) analyses in which HUVEC were treated with COA-Cl either in the presence or absence of pretreatment with PTx. HUVEC were treated with 50 ng mL −1 of PTx overnight, followed by 100 μ mol/L of COA-Cl for 15 min. They were then subjected to IB analyses for phospho- and total-ERK1/2, as described above. (B) BAPTA-AM, a chelator of intracellular calcium (20 μ mol/L for 30 min), was used instead of PTx. (A and B) Representative results of three independent experiments, which yielded equivalent data. (C and D) Results of intracellular Ca 2+ transients assays in HUVEC loaded with fura-2-AM. (C) Representative trace of 1 mmol/L COA-C1-induced intracellular Ca 2+ increase; some cells had been treated with various inhibitors as described above prior to COA-Cl treatment. (D) Summarizes the results derived from pooled records obtained from 8−28 cells in each group. Fura-2 fluorescent signals were recorded and analyzed as described in the main text. Changes in the fura-2 ratio that corresponded to peak increases in intracellular Ca 2+ concentrations are shown as ▵F/F 0 . * P

    Article Snippet: Fura-2 fluorescent signals were recorded (ORCA-Flash 2.8; Hamamatsu Photonics, Hamamatsu, Japan) and analyzed by a ratiometric fluorescence method using MetaFluor software (version 7.7.5.0; Molecular Devices, Sunnyvale, CA).

    Techniques: Derivative Assay

    Impairment of maturation into functional syncytia with synchronously beating cardiomyocytes in TRPV2-deficent newborn mice. ( a ) Isolated cardiomyocytes from TRPV2 flox/flox ;Cre − / − or TRPV2 flox/flox ;Cre +/ − newborn mice. Triple staining with anti-connexin 43 antibody (green), phalloidin (red) and DAPI (blue) in cardiomyocytes (upper panels). Triple staining with anti-N-cadherin antibody (green), phalloidin (red) and DAPI (blue) in cardiomyocytes (middle panels). Triple staining with anti-NCX1 antibody (green), phalloidin (red) and DAPI (blue) in cardiomyocytes (lower panels). Scale bar, 100 μm. ( b ) Fura-2-imaging of stretch-induced intracellular Ca 2+ dynamics in neonatal cardiomyocytes. Scale bar, 100 μm. ( c ) Representative trace of stretch-induced intracellular Ca 2+ increase. ( d ) Effect of mechanosensitive channel inhibitors. Data are means±s.e.m. * P

    Journal: Nature Communications

    Article Title: TRPV2 is critical for the maintenance of cardiac structure and function in mice

    doi: 10.1038/ncomms4932

    Figure Lengend Snippet: Impairment of maturation into functional syncytia with synchronously beating cardiomyocytes in TRPV2-deficent newborn mice. ( a ) Isolated cardiomyocytes from TRPV2 flox/flox ;Cre − / − or TRPV2 flox/flox ;Cre +/ − newborn mice. Triple staining with anti-connexin 43 antibody (green), phalloidin (red) and DAPI (blue) in cardiomyocytes (upper panels). Triple staining with anti-N-cadherin antibody (green), phalloidin (red) and DAPI (blue) in cardiomyocytes (middle panels). Triple staining with anti-NCX1 antibody (green), phalloidin (red) and DAPI (blue) in cardiomyocytes (lower panels). Scale bar, 100 μm. ( b ) Fura-2-imaging of stretch-induced intracellular Ca 2+ dynamics in neonatal cardiomyocytes. Scale bar, 100 μm. ( c ) Representative trace of stretch-induced intracellular Ca 2+ increase. ( d ) Effect of mechanosensitive channel inhibitors. Data are means±s.e.m. * P

    Article Snippet: Fura-2 fluorescent signals were recorded (ORCA-Flash 2.8; Hamamatsu Photonics) and analysed by a ratiometric fluorescence method using MetaFluor software (version 7.7.5.0; Molecular Devices).

    Techniques: Functional Assay, Mouse Assay, Isolation, Staining, Imaging

    Two types of stretch-evoked responses of normal DRG neurons. ( a ) Representative Fura-2 Ca 2+ imaging after stretch stimulation (10 and 100 s) in isolated DRG neurons from TRPV2 flox/flox mice. Arrowheads , fast-decay type; arrows , slow-decay type. ( b ) Typical Ca 2+ response after stretch stimulus in two subsets of neurons. ( c , d ) Stretch threshold of fast- and slow-decay type neurons (fast, n = 23; slow n = 18): Cumulative distribution of the threshold ( c ), median and IQR ( d ) (*** P

    Journal: Scientific Reports

    Article Title: TRPV2 is required for mechanical nociception and the stretch-evoked response of primary sensory neurons

    doi: 10.1038/s41598-018-35049-4

    Figure Lengend Snippet: Two types of stretch-evoked responses of normal DRG neurons. ( a ) Representative Fura-2 Ca 2+ imaging after stretch stimulation (10 and 100 s) in isolated DRG neurons from TRPV2 flox/flox mice. Arrowheads , fast-decay type; arrows , slow-decay type. ( b ) Typical Ca 2+ response after stretch stimulus in two subsets of neurons. ( c , d ) Stretch threshold of fast- and slow-decay type neurons (fast, n = 23; slow n = 18): Cumulative distribution of the threshold ( c ), median and IQR ( d ) (*** P

    Article Snippet: Fura-2 fluorescence signals were recorded at 5 Hz by a digital camera (ORCA-Flash 2.8; Hamamatsu Photonics, Hamamatsu, Japan) and analysed using a ratiometric fluorescence method and MetaFluor software (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Imaging, Isolation, Mouse Assay

    SCA5 β-spectrin dominantly mislocalizes the endogenous spectrin cytoskeleton. ( A , Left ) Confocal images showing the localization of endogenous α-spectrin in the somata and dendrites of class IV da neurons expressing wild-type (WT) or SCA5 β-spectrin together with the fluorescent membrane marker CD4tdtomato. α-Spectrin can also be seen in the somata and dendrites of neighboring sensory neurons not expressing β-spectrin transgenes. (Scale bar, 50 µm.) ( Right ) Quantitation of α-spectrin in cell bodies. P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization

    doi: 10.1073/pnas.1707108114

    Figure Lengend Snippet: SCA5 β-spectrin dominantly mislocalizes the endogenous spectrin cytoskeleton. ( A , Left ) Confocal images showing the localization of endogenous α-spectrin in the somata and dendrites of class IV da neurons expressing wild-type (WT) or SCA5 β-spectrin together with the fluorescent membrane marker CD4tdtomato. α-Spectrin can also be seen in the somata and dendrites of neighboring sensory neurons not expressing β-spectrin transgenes. (Scale bar, 50 µm.) ( Right ) Quantitation of α-spectrin in cell bodies. P

    Article Snippet: CD4tdTom and mEos3.2-β-spectrin fluorescence signals were imaged using a Plan-APOCHROMAT 20×/0.75 objective and an ORCA-ER camera (Hamamatsu) on a spinning-disk confocal microscope (Zeiss).

    Techniques: Expressing, Marker, Quantitation Assay

    SCA5 β-spectrin is absent at da neuron axon terminals. ( Left ) Confocal images of ventral nerve cords showing the axon terminals of class IV da neurons expressing mEos3.2-β-spectrin proteins and the fluorescent membrane marker CD4tdtomato. Wild-type β-spectrin is present in axon terminals, but SCA5 β-spectrin is not. (Scale bar, 100 µm.) ( Right ) Quantitation of mEos3.2 signals at the ventral nerve cord. *** P = 0.0003; n = 5 for wild-type; n = 6 for SCA5. Error bars represent SD.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization

    doi: 10.1073/pnas.1707108114

    Figure Lengend Snippet: SCA5 β-spectrin is absent at da neuron axon terminals. ( Left ) Confocal images of ventral nerve cords showing the axon terminals of class IV da neurons expressing mEos3.2-β-spectrin proteins and the fluorescent membrane marker CD4tdtomato. Wild-type β-spectrin is present in axon terminals, but SCA5 β-spectrin is not. (Scale bar, 100 µm.) ( Right ) Quantitation of mEos3.2 signals at the ventral nerve cord. *** P = 0.0003; n = 5 for wild-type; n = 6 for SCA5. Error bars represent SD.

    Article Snippet: CD4tdTom and mEos3.2-β-spectrin fluorescence signals were imaged using a Plan-APOCHROMAT 20×/0.75 objective and an ORCA-ER camera (Hamamatsu) on a spinning-disk confocal microscope (Zeiss).

    Techniques: Expressing, Marker, Quantitation Assay

    SCA5 β-spectrin is absent in distal dendrites and accumulates in the soma. ( A ) Confocal-image arbor reconstructions of class IV da neurons expressing mEos3.2-β-spectrin wild-type (WT) or SCA5 proteins, together with the fluorescent membrane marker CD4tdtomato. ( Left ) Fluorescence signals of CD4tdtomato and mEos3.2-β-spectrin proteins. Wild-type mEos3.2-β-spectrin localizes throughout arbor and soma. mEos3.2-β-spectrin SCA5 is absent in distal dendrites but is present in proximal dendrites. (Scale bar, 50 µm.) ( Right ) mEos3.2-β-spectrin fluorescence intensities were quantified in 50-µm segments of a primary dendrite (arrowheads in Left ) located 100 µm (dotted line in Left ) from the soma. mEos3.2-β-spectrin SCA5 localization is significantly reduced in the distal regions of primary branches. For wild type, n = 17 neurons (one to three neurons imaged per larva). For SCA5, n = 16 neurons (one to three neurons imaged per larva). *** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization

    doi: 10.1073/pnas.1707108114

    Figure Lengend Snippet: SCA5 β-spectrin is absent in distal dendrites and accumulates in the soma. ( A ) Confocal-image arbor reconstructions of class IV da neurons expressing mEos3.2-β-spectrin wild-type (WT) or SCA5 proteins, together with the fluorescent membrane marker CD4tdtomato. ( Left ) Fluorescence signals of CD4tdtomato and mEos3.2-β-spectrin proteins. Wild-type mEos3.2-β-spectrin localizes throughout arbor and soma. mEos3.2-β-spectrin SCA5 is absent in distal dendrites but is present in proximal dendrites. (Scale bar, 50 µm.) ( Right ) mEos3.2-β-spectrin fluorescence intensities were quantified in 50-µm segments of a primary dendrite (arrowheads in Left ) located 100 µm (dotted line in Left ) from the soma. mEos3.2-β-spectrin SCA5 localization is significantly reduced in the distal regions of primary branches. For wild type, n = 17 neurons (one to three neurons imaged per larva). For SCA5, n = 16 neurons (one to three neurons imaged per larva). *** P

    Article Snippet: CD4tdTom and mEos3.2-β-spectrin fluorescence signals were imaged using a Plan-APOCHROMAT 20×/0.75 objective and an ORCA-ER camera (Hamamatsu) on a spinning-disk confocal microscope (Zeiss).

    Techniques: Expressing, Marker, Fluorescence

    The Drosophila spectrin cytoskeleton is required for dendritic arborization. ( A ) Effect of β-spectrin RNAi on dendritic arborization of class IV da neurons. ( Upper ) Reconstructions of wild-type and β-spectrin RNAi dendritic arbors imaged by fluorescence confocal microscopy in third-instar larvae. In the wild-type arbor, the arrow at left indicates ring-like patterns formed by distal dendrites at body segment boundaries. β-Spectrin RNAi causes a loss of distal dendrites near segment boundaries. (Scale bar, 100 µm.) ( Lower Left ) Sholl analysis plots showing β-spectrin RNAi decreases distal dendritic complexity. For all samples n = 7 neurons. ( Lower Middle ) β-Spectrin RNAi decreases total branch length. *** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization

    doi: 10.1073/pnas.1707108114

    Figure Lengend Snippet: The Drosophila spectrin cytoskeleton is required for dendritic arborization. ( A ) Effect of β-spectrin RNAi on dendritic arborization of class IV da neurons. ( Upper ) Reconstructions of wild-type and β-spectrin RNAi dendritic arbors imaged by fluorescence confocal microscopy in third-instar larvae. In the wild-type arbor, the arrow at left indicates ring-like patterns formed by distal dendrites at body segment boundaries. β-Spectrin RNAi causes a loss of distal dendrites near segment boundaries. (Scale bar, 100 µm.) ( Lower Left ) Sholl analysis plots showing β-spectrin RNAi decreases distal dendritic complexity. For all samples n = 7 neurons. ( Lower Middle ) β-Spectrin RNAi decreases total branch length. *** P

    Article Snippet: CD4tdTom and mEos3.2-β-spectrin fluorescence signals were imaged using a Plan-APOCHROMAT 20×/0.75 objective and an ORCA-ER camera (Hamamatsu) on a spinning-disk confocal microscope (Zeiss).

    Techniques: Fluorescence, Confocal Microscopy