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Carl Zeiss fluorescence signals
Fluorescence Signals, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence signals - by Bioz Stars, 2020-04
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Related Articles

Clone Assay:

Article Title: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Article Snippet: GFP localization of Sc AGU115 protein in Arabidopsis root cell walls The full-length cDNA fused with GFP was cloned into the binary vector described above. .. Fluorescence signals were observed under an Axioplan inverted microscope equipped with a Zeiss LSM780 spectral confocal laser scanning microscope, using a 561 nm laser probe and a 582-670 nm emission filter.

Immunocytochemistry:

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: Paragraph title: Protein extraction, immunoblot analysis, and immunocytochemistry. ... The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Expressing:

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: For marking cultured cortical astrocytes for expression of NBCe1, antibodies against NBCe1 attached to goat-anti-rabbit Alexa Fluor 488 antibody were used (Invitrogen/Molecular Probes). .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Blocking Assay:

Article Title: Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance
Article Snippet: Then each slide was treated with 1 mL 1% blocking reagent (Roche) for 40 min at room temperature. .. Fluorescence signals were analyzed with a Zeiss LSM510 Meta laser scanning microscope (Zeiss, Oberkochen, Germany), and the acquired confocal image stacks were processed by ZEN 2009 software.

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: Fluorescence signals were detected using Zeiss LSM510 confocal microscopes. .. For staining F-actin, cells were fixed with 4% PFA for 10 minutes at RT before blocking.

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: Cells were then incubated with blocking solution containing 10% normal goat serum, 3% bovine serum albumin and 0.1% Triton-X to reduce unspecific antibody binding, for 1 h at RT. .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Article Title: Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Article Snippet: The following day, cells were incubated with culture medium (0.2% FBS) containing 10 μM fluoxetine for 48 h. Then, the cells were fixed with 4% paraformaldehyde for 15 min and permeabilized using 0.5% PBS-T for 5 min. After washing with 0.1% PBS-T, cells were blocked using blocking solution (10% FBS and 0.5% gelatin in PBS) for 30 min. Primary antibody (SQSTM1 and LC3B) was diluted to a concentration of 1:200 and secondary antibody (FITC, Invitrogen) to 1:200. .. Fluorescence signals were detected using a fluorescence confocal microscope (Carl Zeiss, Oberkochen, Germany).

Incubation:

Article Title: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Article Snippet: The seedlings were washed three times in MTSB buffer and blocked with 3% (v/v ) BSA in MTSB buffer for 20 min, followed by labeling with the primary rabbit anti-GFP antibody (TorreyPines Biolabs, NJ, US; TP401) diluted in 1:1000 in 1% (v/v ) BSA in MTSB buffer at 37°C for 2 h. The seedlings were washed two times in MTSB buffer for 5 min followed by incubation with the secondary antibody goat anti-Rabbit IgG H & L conjugated with DyLight 594 (Agrisera, Sweden; AS111815) diluted 1:200 in 1% (v/v ) BSA in MTSB buffer at 37°C for 1 h. The seedlings were washed two times in MTSB buffer for 5 min and mounted in AF1 anti-fadent medium (Citifluor, London, UK). .. Fluorescence signals were observed under an Axioplan inverted microscope equipped with a Zeiss LSM780 spectral confocal laser scanning microscope, using a 561 nm laser probe and a 582-670 nm emission filter.

Article Title: Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance
Article Snippet: Cytox green was diluted to 1:30000 in PBS buffer for nuclei counter staining, and each slide was covered with a 100 µL mixture of these components and incubated for 3 min at room temperature. .. Fluorescence signals were analyzed with a Zeiss LSM510 Meta laser scanning microscope (Zeiss, Oberkochen, Germany), and the acquired confocal image stacks were processed by ZEN 2009 software.

Article Title: Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells
Article Snippet: After washing with PBS, the cells were blocked for 30 min in PBS containing 5% goat serum and 0.2% Triton X-100, then incubated with primary antibody (1:250) at 4 °C overnight, washed extensively, and stained for 6 h with goat anti-rabbit IgG-Texas Red (1:250). .. Fluorescence signals were analyzed using an LSM 800 confocal laser scanning microscope (Carl Zeiss, Germany).

Article Title: ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem
Article Snippet: Ten-day-old seedlings grown on solid MS media were incubated in ½ MS liquid medium containing 10 μM EdU (Click-iT EdU Alexa Fluor 488 imaging kit; Invitrogen) for 16 h. The seedlings were then treated with 90% acetone, washed three times with PBS, fixed with 4% FAA, embedded in Technovit7100 (Heraeus Kulzer), and sectioned using a microtome (LEICA, RM2235). .. The sections were treated with an Alexa Fluor 488 probe according to the manufacturer's protocol, and then fluorescence signals were observed by fluorescence microscopy (Zeiss, Axioimager A2 with FS 38HE filter).

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: Growth media was changed after 24 h, then 2 µM Cell Event™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) was added and cells were incubated in 37 ℃ in the dark for 30 min. .. Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere.

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: Cells were washed with PBS followed by incubation with secondary antibodies for 45 min at RT in dark, then washed several times with PBS and mounted using Vectashield mounting media with DAPI (4,6-diamidino-2-phenylindole; Vector laboratories) to stain nuclei. .. Fluorescence signals were detected using Zeiss LSM510 confocal microscopes.

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: After washing out the primary antibody with PBS, cells were incubated for 1 h with goat-anti rabbit antibody conjugated with Alexa Fluor 488 and Hoechst (5 μg/ml) for 15 min as cell nuclei marker. .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Article Title: Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Article Snippet: The following day, cells were incubated with culture medium (0.2% FBS) containing 10 μM fluoxetine for 48 h. Then, the cells were fixed with 4% paraformaldehyde for 15 min and permeabilized using 0.5% PBS-T for 5 min. After washing with 0.1% PBS-T, cells were blocked using blocking solution (10% FBS and 0.5% gelatin in PBS) for 30 min. Primary antibody (SQSTM1 and LC3B) was diluted to a concentration of 1:200 and secondary antibody (FITC, Invitrogen) to 1:200. .. Fluorescence signals were detected using a fluorescence confocal microscope (Carl Zeiss, Oberkochen, Germany).

Article Title: Improper Tagging of the Non-Essential Small Capsid Protein VP26 Impairs Nuclear Capsid Egress of Herpes Simplex Virus
Article Snippet: After 19.5 h of infection, the fluorescence signals were documented with a Zeiss Axiovert 200 microscope prior to fixation for 1 h at room temperature with 2% (w/v) glutaraldehyde in 130 mM cacodylate buffer at pH 7.4 containing 2 mM CaCl2 and 10 mM MgCl2 . .. The cells were washed and further fixed and contrasted with 1% (w/v) OsO4 in 165 mM cacodylate buffer at pH 7.4 containing 1.5% (w/v) K3 FeIII (CN)6 for 1 h followed by incubation in 0.5% (w/v) uranyl acetate in 50% (v/v) ethanol overnight.

Cell Culture:

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: Immunofluorescence microscopy For immunofluorescence analyses, cells were cultured on glass coverslips. .. Fluorescence signals were detected using Zeiss LSM510 confocal microscopes.

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: For marking cultured cortical astrocytes for expression of NBCe1, antibodies against NBCe1 attached to goat-anti-rabbit Alexa Fluor 488 antibody were used (Invitrogen/Molecular Probes). .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Mass Spectrometry:

Article Title: ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem
Article Snippet: Ten-day-old seedlings grown on solid MS media were incubated in ½ MS liquid medium containing 10 μM EdU (Click-iT EdU Alexa Fluor 488 imaging kit; Invitrogen) for 16 h. The seedlings were then treated with 90% acetone, washed three times with PBS, fixed with 4% FAA, embedded in Technovit7100 (Heraeus Kulzer), and sectioned using a microtome (LEICA, RM2235). .. The sections were treated with an Alexa Fluor 488 probe according to the manufacturer's protocol, and then fluorescence signals were observed by fluorescence microscopy (Zeiss, Axioimager A2 with FS 38HE filter).

Over Expression:

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: Paragraph title: 2.6. Analysis of miR-224 Overexpression Effect on 5-FU Resistance ... Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere.

Electron Microscopy:

Article Title: Improper Tagging of the Non-Essential Small Capsid Protein VP26 Impairs Nuclear Capsid Egress of Herpes Simplex Virus
Article Snippet: Paragraph title: Correlative Light and Electron Microscopy ... After 19.5 h of infection, the fluorescence signals were documented with a Zeiss Axiovert 200 microscope prior to fixation for 1 h at room temperature with 2% (w/v) glutaraldehyde in 130 mM cacodylate buffer at pH 7.4 containing 2 mM CaCl2 and 10 mM MgCl2 .

Transfection:

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: Cells were transfected with mock or miR-224 harboring overexpression vectors using Lipofectamine LTX Transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere.

Infection:

Article Title: Improper Tagging of the Non-Essential Small Capsid Protein VP26 Impairs Nuclear Capsid Egress of Herpes Simplex Virus
Article Snippet: .. After 19.5 h of infection, the fluorescence signals were documented with a Zeiss Axiovert 200 microscope prior to fixation for 1 h at room temperature with 2% (w/v) glutaraldehyde in 130 mM cacodylate buffer at pH 7.4 containing 2 mM CaCl2 and 10 mM MgCl2 . .. The cells were washed and further fixed and contrasted with 1% (w/v) OsO4 in 165 mM cacodylate buffer at pH 7.4 containing 1.5% (w/v) K3 FeIII (CN)6 for 1 h followed by incubation in 0.5% (w/v) uranyl acetate in 50% (v/v) ethanol overnight.

Light Microscopy:

Article Title: Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells
Article Snippet: .. 4.2 CLEM with pre-embedding light microscopy Cells were grown on gridded glass coverslips in MatTek dishes (MatTek Corporation, Ashland, MA, USA), fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4, and mounted in Mowiol/DABCO prior to imaging for brightfield and fluorescence signals on an inverted LSM 710 confocal microscope with a 63×/1.4 objective (Zeiss, Cambridge). ..

Imaging:

Article Title: Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells
Article Snippet: .. 4.2 CLEM with pre-embedding light microscopy Cells were grown on gridded glass coverslips in MatTek dishes (MatTek Corporation, Ashland, MA, USA), fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4, and mounted in Mowiol/DABCO prior to imaging for brightfield and fluorescence signals on an inverted LSM 710 confocal microscope with a 63×/1.4 objective (Zeiss, Cambridge). ..

Article Title: ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem
Article Snippet: Ten-day-old seedlings grown on solid MS media were incubated in ½ MS liquid medium containing 10 μM EdU (Click-iT EdU Alexa Fluor 488 imaging kit; Invitrogen) for 16 h. The seedlings were then treated with 90% acetone, washed three times with PBS, fixed with 4% FAA, embedded in Technovit7100 (Heraeus Kulzer), and sectioned using a microtome (LEICA, RM2235). .. The sections were treated with an Alexa Fluor 488 probe according to the manufacturer's protocol, and then fluorescence signals were observed by fluorescence microscopy (Zeiss, Axioimager A2 with FS 38HE filter).

Inverted Microscopy:

Article Title: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Article Snippet: .. Fluorescence signals were observed under an Axioplan inverted microscope equipped with a Zeiss LSM780 spectral confocal laser scanning microscope, using a 561 nm laser probe and a 582-670 nm emission filter. ..

Immunostaining:

Article Title: Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Article Snippet: Paragraph title: 3.7. Immunostaining of SQSTM1 and LC3B ... Fluorescence signals were detected using a fluorescence confocal microscope (Carl Zeiss, Oberkochen, Germany).

Binding Assay:

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: Cells were then incubated with blocking solution containing 10% normal goat serum, 3% bovine serum albumin and 0.1% Triton-X to reduce unspecific antibody binding, for 1 h at RT. .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Immunofluorescence:

Article Title: Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells
Article Snippet: Paragraph title: Immunofluorescence ... Fluorescence signals were analyzed using an LSM 800 confocal laser scanning microscope (Carl Zeiss, Germany).

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: Paragraph title: Immunofluorescence microscopy ... Fluorescence signals were detected using Zeiss LSM510 confocal microscopes.

Fluorescence:

Article Title: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Article Snippet: .. Fluorescence signals were observed under an Axioplan inverted microscope equipped with a Zeiss LSM780 spectral confocal laser scanning microscope, using a 561 nm laser probe and a 582-670 nm emission filter. ..

Article Title: Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance
Article Snippet: .. Fluorescence signals were analyzed with a Zeiss LSM510 Meta laser scanning microscope (Zeiss, Oberkochen, Germany), and the acquired confocal image stacks were processed by ZEN 2009 software. ..

Article Title: Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells
Article Snippet: .. 4.2 CLEM with pre-embedding light microscopy Cells were grown on gridded glass coverslips in MatTek dishes (MatTek Corporation, Ashland, MA, USA), fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4, and mounted in Mowiol/DABCO prior to imaging for brightfield and fluorescence signals on an inverted LSM 710 confocal microscope with a 63×/1.4 objective (Zeiss, Cambridge). ..

Article Title: Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells
Article Snippet: .. Fluorescence signals were analyzed using an LSM 800 confocal laser scanning microscope (Carl Zeiss, Germany). .. Cells (1 × 106 cells/well) were cotransfected with 2.5 μg of human COX-2 promoter luciferase constructs and pRL-renilla vectors (Promega, Madison, WI), according to the manufacturer’s protocol, by using the Neon™ transfection system.

Article Title: ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem
Article Snippet: .. The sections were treated with an Alexa Fluor 488 probe according to the manufacturer's protocol, and then fluorescence signals were observed by fluorescence microscopy (Zeiss, Axioimager A2 with FS 38HE filter). ..

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: .. Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere. .. Two fluorescence signals were registered: green (Cell Event dye—cells with activated caspase 3/7) and red (mCherry dye—a marker for miRNA-transfected cells).

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: .. Fluorescence signals were detected using Zeiss LSM510 confocal microscopes. .. For staining F-actin, cells were fixed with 4% PFA for 10 minutes at RT before blocking.

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss). .. To measure the intracellular H+ concentration ([H+ ]i ) changes in cultured cortical astrocytes, we used a confocal imaging system and acetoxymethyl ester of a proton-sensitive dye, BCECF-AM.

Article Title: Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Article Snippet: .. Fluorescence signals were detected using a fluorescence confocal microscope (Carl Zeiss, Oberkochen, Germany). .. RNA Isolation and qPCR Total cellular RNA was extracted using an RNA prep kit (RNeasy™, Qiagen) and quantificated using Nanodrop (Thermo Scientific).

Article Title: Improper Tagging of the Non-Essential Small Capsid Protein VP26 Impairs Nuclear Capsid Egress of Herpes Simplex Virus
Article Snippet: .. After 19.5 h of infection, the fluorescence signals were documented with a Zeiss Axiovert 200 microscope prior to fixation for 1 h at room temperature with 2% (w/v) glutaraldehyde in 130 mM cacodylate buffer at pH 7.4 containing 2 mM CaCl2 and 10 mM MgCl2 . .. The cells were washed and further fixed and contrasted with 1% (w/v) OsO4 in 165 mM cacodylate buffer at pH 7.4 containing 1.5% (w/v) K3 FeIII (CN)6 for 1 h followed by incubation in 0.5% (w/v) uranyl acetate in 50% (v/v) ethanol overnight.

Article Title: Tracheophytes Contain Conserved Orthologs of a Basic Helix-Loop-Helix Transcription Factor That Modulate ROOT HAIR SPECIFIC Genes [OPEN]
Article Snippet: .. Fluorescence signals of the GFP-fusion proteins from 4-d-old transgenic seedlings were observed using an LSM 510 confocal laser scanning microscope (Carl Zeiss). .. Four-day-old transgenic seedlings expressing the GFP-fusion proteins were vacuum-infiltrated in 1% formaldehyde solution for cross-linking.

Microscopy:

Article Title: Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells
Article Snippet: .. 4.2 CLEM with pre-embedding light microscopy Cells were grown on gridded glass coverslips in MatTek dishes (MatTek Corporation, Ashland, MA, USA), fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4, and mounted in Mowiol/DABCO prior to imaging for brightfield and fluorescence signals on an inverted LSM 710 confocal microscope with a 63×/1.4 objective (Zeiss, Cambridge). ..

Article Title: ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem
Article Snippet: .. The sections were treated with an Alexa Fluor 488 probe according to the manufacturer's protocol, and then fluorescence signals were observed by fluorescence microscopy (Zeiss, Axioimager A2 with FS 38HE filter). ..

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: Analysis of miR-224 Overexpression Effect on 5-FU Resistance HCT-FU-c cell line cells (2 × 105 cells/well) were seeded on the glass surface of a microscopy holder 24 h prior to transfection. .. Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere.

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: Paragraph title: Immunofluorescence microscopy ... Fluorescence signals were detected using Zeiss LSM510 confocal microscopes.

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss). .. To measure the intracellular H+ concentration ([H+ ]i ) changes in cultured cortical astrocytes, we used a confocal imaging system and acetoxymethyl ester of a proton-sensitive dye, BCECF-AM.

Article Title: Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Article Snippet: .. Fluorescence signals were detected using a fluorescence confocal microscope (Carl Zeiss, Oberkochen, Germany). .. RNA Isolation and qPCR Total cellular RNA was extracted using an RNA prep kit (RNeasy™, Qiagen) and quantificated using Nanodrop (Thermo Scientific).

Article Title: Improper Tagging of the Non-Essential Small Capsid Protein VP26 Impairs Nuclear Capsid Egress of Herpes Simplex Virus
Article Snippet: .. After 19.5 h of infection, the fluorescence signals were documented with a Zeiss Axiovert 200 microscope prior to fixation for 1 h at room temperature with 2% (w/v) glutaraldehyde in 130 mM cacodylate buffer at pH 7.4 containing 2 mM CaCl2 and 10 mM MgCl2 . .. The cells were washed and further fixed and contrasted with 1% (w/v) OsO4 in 165 mM cacodylate buffer at pH 7.4 containing 1.5% (w/v) K3 FeIII (CN)6 for 1 h followed by incubation in 0.5% (w/v) uranyl acetate in 50% (v/v) ethanol overnight.

Article Title: Tracheophytes Contain Conserved Orthologs of a Basic Helix-Loop-Helix Transcription Factor That Modulate ROOT HAIR SPECIFIC Genes [OPEN]
Article Snippet: Paragraph title: Microscopy Observation of Fluorescent Proteins ... Fluorescence signals of the GFP-fusion proteins from 4-d-old transgenic seedlings were observed using an LSM 510 confocal laser scanning microscope (Carl Zeiss).

Transgenic Assay:

Article Title: Tracheophytes Contain Conserved Orthologs of a Basic Helix-Loop-Helix Transcription Factor That Modulate ROOT HAIR SPECIFIC Genes [OPEN]
Article Snippet: .. Fluorescence signals of the GFP-fusion proteins from 4-d-old transgenic seedlings were observed using an LSM 510 confocal laser scanning microscope (Carl Zeiss). .. Four-day-old transgenic seedlings expressing the GFP-fusion proteins were vacuum-infiltrated in 1% formaldehyde solution for cross-linking.

Protein Extraction:

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: Paragraph title: Protein extraction, immunoblot analysis, and immunocytochemistry. ... The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Labeling:

Article Title: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Article Snippet: The seedlings were washed three times in MTSB buffer and blocked with 3% (v/v ) BSA in MTSB buffer for 20 min, followed by labeling with the primary rabbit anti-GFP antibody (TorreyPines Biolabs, NJ, US; TP401) diluted in 1:1000 in 1% (v/v ) BSA in MTSB buffer at 37°C for 2 h. The seedlings were washed two times in MTSB buffer for 5 min followed by incubation with the secondary antibody goat anti-Rabbit IgG H & L conjugated with DyLight 594 (Agrisera, Sweden; AS111815) diluted 1:200 in 1% (v/v ) BSA in MTSB buffer at 37°C for 1 h. The seedlings were washed two times in MTSB buffer for 5 min and mounted in AF1 anti-fadent medium (Citifluor, London, UK). .. Fluorescence signals were observed under an Axioplan inverted microscope equipped with a Zeiss LSM780 spectral confocal laser scanning microscope, using a 561 nm laser probe and a 582-670 nm emission filter.

Article Title: ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem
Article Snippet: Paragraph title: EdU labeling assay ... The sections were treated with an Alexa Fluor 488 probe according to the manufacturer's protocol, and then fluorescence signals were observed by fluorescence microscopy (Zeiss, Axioimager A2 with FS 38HE filter).

In Situ Hybridization:

Article Title: Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance
Article Snippet: Paragraph title: In situ hybridization ... Fluorescence signals were analyzed with a Zeiss LSM510 Meta laser scanning microscope (Zeiss, Oberkochen, Germany), and the acquired confocal image stacks were processed by ZEN 2009 software.

Plasmid Preparation:

Article Title: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Article Snippet: GFP localization of Sc AGU115 protein in Arabidopsis root cell walls The full-length cDNA fused with GFP was cloned into the binary vector described above. .. Fluorescence signals were observed under an Axioplan inverted microscope equipped with a Zeiss LSM780 spectral confocal laser scanning microscope, using a 561 nm laser probe and a 582-670 nm emission filter.

Software:

Article Title: Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance
Article Snippet: .. Fluorescence signals were analyzed with a Zeiss LSM510 Meta laser scanning microscope (Zeiss, Oberkochen, Germany), and the acquired confocal image stacks were processed by ZEN 2009 software. ..

Laser-Scanning Microscopy:

Article Title: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
Article Snippet: .. Fluorescence signals were observed under an Axioplan inverted microscope equipped with a Zeiss LSM780 spectral confocal laser scanning microscope, using a 561 nm laser probe and a 582-670 nm emission filter. ..

Article Title: Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance
Article Snippet: .. Fluorescence signals were analyzed with a Zeiss LSM510 Meta laser scanning microscope (Zeiss, Oberkochen, Germany), and the acquired confocal image stacks were processed by ZEN 2009 software. ..

Article Title: Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells
Article Snippet: .. Fluorescence signals were analyzed using an LSM 800 confocal laser scanning microscope (Carl Zeiss, Germany). .. Cells (1 × 106 cells/well) were cotransfected with 2.5 μg of human COX-2 promoter luciferase constructs and pRL-renilla vectors (Promega, Madison, WI), according to the manufacturer’s protocol, by using the Neon™ transfection system.

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: .. Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere. .. Two fluorescence signals were registered: green (Cell Event dye—cells with activated caspase 3/7) and red (mCherry dye—a marker for miRNA-transfected cells).

Article Title: Tracheophytes Contain Conserved Orthologs of a Basic Helix-Loop-Helix Transcription Factor That Modulate ROOT HAIR SPECIFIC Genes [OPEN]
Article Snippet: .. Fluorescence signals of the GFP-fusion proteins from 4-d-old transgenic seedlings were observed using an LSM 510 confocal laser scanning microscope (Carl Zeiss). .. Four-day-old transgenic seedlings expressing the GFP-fusion proteins were vacuum-infiltrated in 1% formaldehyde solution for cross-linking.

Knock-Out:

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: Fluorescence signals were detected using Zeiss LSM510 confocal microscopes. .. The RPGR knockout mouse model was gifted from Professor Wright’s group at MRC Human Genetics Unit, Edinburgh, UK.

Concentration Assay:

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: .. Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere. .. Two fluorescence signals were registered: green (Cell Event dye—cells with activated caspase 3/7) and red (mCherry dye—a marker for miRNA-transfected cells).

Article Title: Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Article Snippet: The following day, cells were incubated with culture medium (0.2% FBS) containing 10 μM fluoxetine for 48 h. Then, the cells were fixed with 4% paraformaldehyde for 15 min and permeabilized using 0.5% PBS-T for 5 min. After washing with 0.1% PBS-T, cells were blocked using blocking solution (10% FBS and 0.5% gelatin in PBS) for 30 min. Primary antibody (SQSTM1 and LC3B) was diluted to a concentration of 1:200 and secondary antibody (FITC, Invitrogen) to 1:200. .. Fluorescence signals were detected using a fluorescence confocal microscope (Carl Zeiss, Oberkochen, Germany).

Marker:

Article Title: Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial–Mesenchymal Transition of Colorectal Cancer Cells
Article Snippet: Cells were treated with 25 µM 5-FU (the concentration is comparable to IC20 of HCT-FU-c cell line) and fluorescence signals of 5–9 different locations were registered in 15 min intervals for 48 h. The fluorescence of cells was registered with laser scanning microscope ZEISS LSM 710 using 20 × 1.25 N/A lenses at 37 ℃, 5% CO2 and 95% humidity atmosphere. .. Two fluorescence signals were registered: green (Cell Event dye—cells with activated caspase 3/7) and red (mCherry dye—a marker for miRNA-transfected cells).

Article Title: The Electrogenic Sodium Bicarbonate Cotransporter NBCe1 Is a High-Affinity Bicarbonate Carrier in Cortical Astrocytes
Article Snippet: After washing out the primary antibody with PBS, cells were incubated for 1 h with goat-anti rabbit antibody conjugated with Alexa Fluor 488 and Hoechst (5 μg/ml) for 15 min as cell nuclei marker. .. The fluorescence signals were detected by using a LSM-710 confocal microscope (Zeiss).

Staining:

Article Title: Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance
Article Snippet: Cytox green was diluted to 1:30000 in PBS buffer for nuclei counter staining, and each slide was covered with a 100 µL mixture of these components and incubated for 3 min at room temperature. .. Fluorescence signals were analyzed with a Zeiss LSM510 Meta laser scanning microscope (Zeiss, Oberkochen, Germany), and the acquired confocal image stacks were processed by ZEN 2009 software.

Article Title: Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells
Article Snippet: After washing with PBS, the cells were blocked for 30 min in PBS containing 5% goat serum and 0.2% Triton X-100, then incubated with primary antibody (1:250) at 4 °C overnight, washed extensively, and stained for 6 h with goat anti-rabbit IgG-Texas Red (1:250). .. Fluorescence signals were analyzed using an LSM 800 confocal laser scanning microscope (Carl Zeiss, Germany).

Article Title: RPGR protein complex regulates proteasome activity and mediates store-operated calcium entry
Article Snippet: Cells were washed with PBS followed by incubation with secondary antibodies for 45 min at RT in dark, then washed several times with PBS and mounted using Vectashield mounting media with DAPI (4,6-diamidino-2-phenylindole; Vector laboratories) to stain nuclei. .. Fluorescence signals were detected using Zeiss LSM510 confocal microscopes.

Article Title: Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Article Snippet: For nuclear staining, cells were stained with DAPI (Sigma, D9542). .. Fluorescence signals were detected using a fluorescence confocal microscope (Carl Zeiss, Oberkochen, Germany).

Epifluorescence Microscopy:

Article Title: Improper Tagging of the Non-Essential Small Capsid Protein VP26 Impairs Nuclear Capsid Egress of Herpes Simplex Virus
Article Snippet: After 19.5 h of infection, the fluorescence signals were documented with a Zeiss Axiovert 200 microscope prior to fixation for 1 h at room temperature with 2% (w/v) glutaraldehyde in 130 mM cacodylate buffer at pH 7.4 containing 2 mM CaCl2 and 10 mM MgCl2 . .. The cells were flat-embedded in Epon, and semi-thin sections cut parallel to the substrate were used to identify cells characterized by a high amount of nuclear aggregates and an indicative nuclear morphology that had been documented by epifluorescence microscopy prior to fixation.

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  • 84
    Carl Zeiss 4 hne fluorescent signal
    H 2 water reduced <t>4-HNE,</t> but not DHE fluorescence in SN after MPTP administration. (A) Fluorescent images of 4-HNE, DHE, and TH in SN (n = 3∼4). Mice treated with non-H 2 water or H 2 /Mg water with injection of saline or MPTP. All samples were acquired 24 h after administration of saline or MPTP. Scale: 50 µm. (B, C) Quantification of 4-HNE (B) and DHE (C) intensity in TH-positive cells in SN. One-way ANOVA; # P
    4 Hne Fluorescent Signal, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss fluorescence in situ hybridization signals
    <t>Fluorescence</t> <t>in</t> <t>situ</t> <t>hybridization.</t> a, The distribution on a normal X chromosome of the STS locus at the distal tip of the short arm in red, the X centromere (DXZ1) in green, and the proximal long arm signal of XIST in red. This cell has a single X chromosome. b, A cell with an X,r(X) complement showing the 3 expected <t>signals</t> on the normal X chromosome, and an X centromere signal and the proximal XIST locus on the ring (arrow). c, A cell with an X,der(X)t(X;X) complement showing the 3 expected signals on the normal X, and only the X centromere signal and the proximal XIST locus on the larger and more metacentric der(X)t(X;X) chromosome (arrow). The STS locus on the short arm of the der(X)t(X;X) chromosome has been lost in the rearrangement.
    Fluorescence In Situ Hybridization Signals, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss ih gfp fluorescence signals
    Rice NADP-ME2 Is Involved in the Accumulation of ROS and Ferric Ions (Fe 3+ ) in HR Cell Death during Avirulent M. oryzae Infection. (A) and (B) CM-H 2 <t>DCFDA</t> (GF) (A) and Prussian blue (blue color) (B) staining shows the accumulation of H 2 O 2 and ferric ion (Fe 3+ ) around IH in wild-type rice (cv HY) during avirulent M. oryzae INA168 infection. By contrast, the focal accumulation of H 2 O 2 and ferric ion (Fe 3+ ) was not detected in ΔOs-nadp-me2-3 mutant rice. (C) Avirulent M. oryzae <t>INA168:GFP</t> induces HR cell death in wild-type rice but successfully colonizes ΔOs-nadp-me2-3 mutant rice. Images in (A) and (C) were taken by a fluorescence microscope (Zeiss equipped with Axioplan 2) using a bright field (BF) as well as a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters. Images in (B) were taken by a fluorescence microscope using a bright field. The images shown are representative of the different leaf sheath samples that were analyzed in three independent experiments. Bars = 20 μm.
    Ih Gfp Fluorescence Signals, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Zeiss fluorescent tunel signal
    Apoptosis in ciliates. <t>TUNEL</t> assay in E. aediculatus treated with 2 μg/mL AN2 or vehicle (CTRL) for 2 h. <t>DAPI</t> (blue) was used for nuclei detection. The images are representative of 10 independent experiments. Scale bar = 30 µm.
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    Image Search Results


    H 2 water reduced 4-HNE, but not DHE fluorescence in SN after MPTP administration. (A) Fluorescent images of 4-HNE, DHE, and TH in SN (n = 3∼4). Mice treated with non-H 2 water or H 2 /Mg water with injection of saline or MPTP. All samples were acquired 24 h after administration of saline or MPTP. Scale: 50 µm. (B, C) Quantification of 4-HNE (B) and DHE (C) intensity in TH-positive cells in SN. One-way ANOVA; # P

    Journal: PLoS ONE

    Article Title: Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Mouse Model of Parkinson's Disease

    doi: 10.1371/journal.pone.0007247

    Figure Lengend Snippet: H 2 water reduced 4-HNE, but not DHE fluorescence in SN after MPTP administration. (A) Fluorescent images of 4-HNE, DHE, and TH in SN (n = 3∼4). Mice treated with non-H 2 water or H 2 /Mg water with injection of saline or MPTP. All samples were acquired 24 h after administration of saline or MPTP. Scale: 50 µm. (B, C) Quantification of 4-HNE (B) and DHE (C) intensity in TH-positive cells in SN. One-way ANOVA; # P

    Article Snippet: Quantitative analysis of DHE and 4-HNE fluorescent signal All samples were analyzed with a confocal laser scanning microscope (LSM510META, Carl Zeiss, Germany).

    Techniques: Fluorescence, Mouse Assay, Injection

    Fluorescence in situ hybridization. a, The distribution on a normal X chromosome of the STS locus at the distal tip of the short arm in red, the X centromere (DXZ1) in green, and the proximal long arm signal of XIST in red. This cell has a single X chromosome. b, A cell with an X,r(X) complement showing the 3 expected signals on the normal X chromosome, and an X centromere signal and the proximal XIST locus on the ring (arrow). c, A cell with an X,der(X)t(X;X) complement showing the 3 expected signals on the normal X, and only the X centromere signal and the proximal XIST locus on the larger and more metacentric der(X)t(X;X) chromosome (arrow). The STS locus on the short arm of the der(X)t(X;X) chromosome has been lost in the rearrangement.

    Journal: Archives of pathology & laboratory medicine

    Article Title: Extensive Analysis of Mosaicism in a Case of Turner Syndrome

    doi: 10.1043/0003-9985(1999)123 < 0381:EAOMIA > 2.0.CO;2

    Figure Lengend Snippet: Fluorescence in situ hybridization. a, The distribution on a normal X chromosome of the STS locus at the distal tip of the short arm in red, the X centromere (DXZ1) in green, and the proximal long arm signal of XIST in red. This cell has a single X chromosome. b, A cell with an X,r(X) complement showing the 3 expected signals on the normal X chromosome, and an X centromere signal and the proximal XIST locus on the ring (arrow). c, A cell with an X,der(X)t(X;X) complement showing the 3 expected signals on the normal X, and only the X centromere signal and the proximal XIST locus on the larger and more metacentric der(X)t(X;X) chromosome (arrow). The STS locus on the short arm of the der(X)t(X;X) chromosome has been lost in the rearrangement.

    Article Snippet: Fluorescence in situ hybridization signals were captured using a monochromatic CCD camera mounted on a Zeiss epifluorescence microscope with an LUDL filter wheel and a fixed, multi-band-pass beam splitter using MacProbe software (Perceptive Scientific Instruments, Houston, Tex).

    Techniques: Fluorescence, In Situ Hybridization

    Rice NADP-ME2 Is Involved in the Accumulation of ROS and Ferric Ions (Fe 3+ ) in HR Cell Death during Avirulent M. oryzae Infection. (A) and (B) CM-H 2 DCFDA (GF) (A) and Prussian blue (blue color) (B) staining shows the accumulation of H 2 O 2 and ferric ion (Fe 3+ ) around IH in wild-type rice (cv HY) during avirulent M. oryzae INA168 infection. By contrast, the focal accumulation of H 2 O 2 and ferric ion (Fe 3+ ) was not detected in ΔOs-nadp-me2-3 mutant rice. (C) Avirulent M. oryzae INA168:GFP induces HR cell death in wild-type rice but successfully colonizes ΔOs-nadp-me2-3 mutant rice. Images in (A) and (C) were taken by a fluorescence microscope (Zeiss equipped with Axioplan 2) using a bright field (BF) as well as a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters. Images in (B) were taken by a fluorescence microscope using a bright field. The images shown are representative of the different leaf sheath samples that were analyzed in three independent experiments. Bars = 20 μm.

    Journal: The Plant Cell

    Article Title: Iron- and Reactive Oxygen Species-Dependent Ferroptotic Cell Death in Rice-Magnaporthe oryzae Interactions

    doi: 10.1105/tpc.18.00535

    Figure Lengend Snippet: Rice NADP-ME2 Is Involved in the Accumulation of ROS and Ferric Ions (Fe 3+ ) in HR Cell Death during Avirulent M. oryzae Infection. (A) and (B) CM-H 2 DCFDA (GF) (A) and Prussian blue (blue color) (B) staining shows the accumulation of H 2 O 2 and ferric ion (Fe 3+ ) around IH in wild-type rice (cv HY) during avirulent M. oryzae INA168 infection. By contrast, the focal accumulation of H 2 O 2 and ferric ion (Fe 3+ ) was not detected in ΔOs-nadp-me2-3 mutant rice. (C) Avirulent M. oryzae INA168:GFP induces HR cell death in wild-type rice but successfully colonizes ΔOs-nadp-me2-3 mutant rice. Images in (A) and (C) were taken by a fluorescence microscope (Zeiss equipped with Axioplan 2) using a bright field (BF) as well as a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters. Images in (B) were taken by a fluorescence microscope using a bright field. The images shown are representative of the different leaf sheath samples that were analyzed in three independent experiments. Bars = 20 μm.

    Article Snippet: Both CM-H2 DCFDA-specific and IH:GFP fluorescence signals were visualized with the Zeiss fluorescence microscope using a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters.

    Techniques: Infection, Staining, Mutagenesis, Fluorescence, Microscopy

    Apoptosis in ciliates. TUNEL assay in E. aediculatus treated with 2 μg/mL AN2 or vehicle (CTRL) for 2 h. DAPI (blue) was used for nuclei detection. The images are representative of 10 independent experiments. Scale bar = 30 µm.

    Journal: Toxins

    Article Title: Bioactivity and Structural Properties of Novel Synthetic Analogues of the Protozoan Toxin Climacostol

    doi: 10.3390/toxins11010042

    Figure Lengend Snippet: Apoptosis in ciliates. TUNEL assay in E. aediculatus treated with 2 μg/mL AN2 or vehicle (CTRL) for 2 h. DAPI (blue) was used for nuclei detection. The images are representative of 10 independent experiments. Scale bar = 30 µm.

    Article Snippet: DAPI staining, fluorescent TUNEL signal, and the corresponding bright field images were acquired using a Zeiss microscope (Axioskop 2 plus, Carl Zeiss, Oberkochen, Germany) equipped with the Axiocam MRC photocamera and the Axiovision software.

    Techniques: TUNEL Assay