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Becton Dickinson fluorescence signals
Fluorescence Signals, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Centrifugation:

Article Title: Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1 2
Article Snippet: Blood samples were freshly collected, and red blood cells were eliminated by centrifugation on Histopaque 1083 (Sigma). .. Fluorescence signals were measured using a FACScan apparatus (BD Pharmingen) and analyzed by the FlowJo software (Tree Star, Inc, Olten, Switzerland).

Luciferase:

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: Paragraph title: Luciferase reporter assay ... Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK).

Reporter Assay:

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: Paragraph title: Luciferase reporter assay ... Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK).

Cytometry:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: .. Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle. .. FACS enrichment of DNA-carrying beads was carried out by collecting beads from the fluorescent population into a microcentrifuge tube containing 150 μl 1×TE (10 mM Tris–HCl, pH 7.5, 1 mM EDTA).

Article Title: Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes
Article Snippet: For analysis through flow cytometry, cells were harvested using trypsin–EDTA and pellets were resuspended in 100 uL of RPMI 1640 medium (Hyclone) containing 5% FBS and pH-adjusted to 7.2. .. Fluorescence signals from the peptide-SLA class II complexes was quantified by FACScalibur (BD Bioscience, San Jose, CA, USA) with CellQuest software.

Article Title: Engineering modular intracellular protein sensor-actuator devices
Article Snippet: Fluorescence signals were then quantified with a flow-cytometer BD LSR-II system (Becton Dickinson) and FACSDiva8 software with the following settings: 640 nm laser and 670/14 nm filter. .. Flow cytometry data were converted from arbitrary units to compensated molecules of equivalent soluble fluorochrome (MESF) using Spherotech #RCP-30- 5A-2.

Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
Article Snippet: .. Finally, the fluorescence signals of DCFH-DA was read at 488 nm excitation and 525 nm emission by the BD FACS Vantage SE Flow Cytometer (BD), and DHE was read at 535 nm excitation and 610 nm emission by a microplate reader (Thermo Fischer). .. ELISA The concentrations of neutrophil elastase (NE) in the serum of human peripheral blood were assayed with an ELISA kit (JYM, 1078, China) according to the manufacturer's instructions.

Article Title: ER membrane protein complex subunit 6 (EMC6) is a novel tumor suppressor in gastric cancer
Article Snippet: .. Fluorescence signals were detected through a FACSCalibur flow cytometer (BD Biosciences, Franklinlake, NJ, USA) to determine the percentage of apoptotic cells, which included Annexin V+ PI+ double and Annexin V+ single positive cells. .. Cell cycle analysis BGC823 cells were infected with either Ad5-Null or Ad5-EMC6 for 24 to 96 h. The cells were fixed with 70% ethanol at −12°C overnight, then treated with 100 μg/ml RNase A for 30 min at 37°C, and stained using PI in 0.2% Triton X-100.

Article Title: Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1 2
Article Snippet: Paragraph title: Flow Cytometry Analysis ... Fluorescence signals were measured using a FACScan apparatus (BD Pharmingen) and analyzed by the FlowJo software (Tree Star, Inc, Olten, Switzerland).

Article Title: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
Article Snippet: .. Intensities of fluorescence signals were measured on a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA). .. The percentages of cells in the G0/G1, S, and G2/M phases were determined using FlowJo software (Tree Star).

Article Title: HSP90 Chaperoning in Addition to Phosphoprotein Required for Folding but Not for Supporting Enzymatic Activities of Measles and Nipah Virus L Polymerases
Article Snippet: Paragraph title: Flow cytometry. ... Fluorescence signals were quantified with Accuri C6 (BD Biosciences).

Article Title: Antiphotoaging and Antimelanogenic Effects of Penthorum chinense Pursh Ethanol Extract due to Antioxidant- and Autophagy-Inducing Properties
Article Snippet: .. Fluorescence signals from the cells were measured using a BD FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA) and CellQuest Pro (IVD) software (Becton Dickinson). ..

Article Title: Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis
Article Snippet: .. Fluorescence signals of the beads were acquired by a flow cytometer (FACS Canto II, Becton Dickinson). ..

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: To take protein expression variations into account, flow cytometry was performed contemporaneously with the luciferase assay. .. Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK).

SYBR Green Assay:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: .. Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle. .. FACS enrichment of DNA-carrying beads was carried out by collecting beads from the fluorescent population into a microcentrifuge tube containing 150 μl 1×TE (10 mM Tris–HCl, pH 7.5, 1 mM EDTA).

Incubation:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: Samples were incubated at room temperature for 10 min in the dark, then transferred to FACS tubes and diluted by adding 500 μl PBSP for subsequent flow cytometric analysis. .. Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle.

Article Title: Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes
Article Snippet: Cell pellets were resuspended in a 1:40 dilution of streptavidin-FITC conjugate (Thermo Fisher Scientific, Massachusetts, USA) in the wash buffer and then incubated on ice for 30 min. .. Fluorescence signals from the peptide-SLA class II complexes was quantified by FACScalibur (BD Bioscience, San Jose, CA, USA) with CellQuest software.

Article Title: Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis
Article Snippet: Briefly, capture antibody-conjugated beads were first incubated with supernatants or standard controls for 60 minutes, then with biotinylated detection antibodies for 30 minutes, and finally with streptavidin-PE for 20 minutes. .. Fluorescence signals of the beads were acquired by a flow cytometer (FACS Canto II, Becton Dickinson).

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: Cells were then washed once in ice-cold PBS, and incubated with PE conjugated anti-mouse secondary antibodies for 1 hour at 4°C. .. Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK).

Cell Culture:

Article Title: Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis
Article Snippet: The cytokine levels in cell-culture supernatants were quantified by AimPlex Human Th1/Th2/Th17 7-Plex assay kits (CliniSciences, Nanterre, France) according to the manufacturer’s instruction manual. .. Fluorescence signals of the beads were acquired by a flow cytometer (FACS Canto II, Becton Dickinson).

Expressing:

Article Title: Engineering modular intracellular protein sensor-actuator devices
Article Snippet: Paragraph title: HLA-I surface expression ... Fluorescence signals were then quantified with a flow-cytometer BD LSR-II system (Becton Dickinson) and FACSDiva8 software with the following settings: 640 nm laser and 670/14 nm filter.

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: To take protein expression variations into account, flow cytometry was performed contemporaneously with the luciferase assay. .. Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK).

Flow Cytometry:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: .. Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle. .. FACS enrichment of DNA-carrying beads was carried out by collecting beads from the fluorescent population into a microcentrifuge tube containing 150 μl 1×TE (10 mM Tris–HCl, pH 7.5, 1 mM EDTA).

Article Title: Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes
Article Snippet: For analysis through flow cytometry, cells were harvested using trypsin–EDTA and pellets were resuspended in 100 uL of RPMI 1640 medium (Hyclone) containing 5% FBS and pH-adjusted to 7.2. .. Fluorescence signals from the peptide-SLA class II complexes was quantified by FACScalibur (BD Bioscience, San Jose, CA, USA) with CellQuest software.

Article Title: Engineering modular intracellular protein sensor-actuator devices
Article Snippet: .. Fluorescence signals were then quantified with a flow-cytometer BD LSR-II system (Becton Dickinson) and FACSDiva8 software with the following settings: 640 nm laser and 670/14 nm filter. .. Flow cytometry data were converted from arbitrary units to compensated molecules of equivalent soluble fluorochrome (MESF) using Spherotech #RCP-30- 5A-2.

Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
Article Snippet: .. Finally, the fluorescence signals of DCFH-DA was read at 488 nm excitation and 525 nm emission by the BD FACS Vantage SE Flow Cytometer (BD), and DHE was read at 535 nm excitation and 610 nm emission by a microplate reader (Thermo Fischer). .. ELISA The concentrations of neutrophil elastase (NE) in the serum of human peripheral blood were assayed with an ELISA kit (JYM, 1078, China) according to the manufacturer's instructions.

Article Title: ER membrane protein complex subunit 6 (EMC6) is a novel tumor suppressor in gastric cancer
Article Snippet: .. Fluorescence signals were detected through a FACSCalibur flow cytometer (BD Biosciences, Franklinlake, NJ, USA) to determine the percentage of apoptotic cells, which included Annexin V+ PI+ double and Annexin V+ single positive cells. .. Cell cycle analysis BGC823 cells were infected with either Ad5-Null or Ad5-EMC6 for 24 to 96 h. The cells were fixed with 70% ethanol at −12°C overnight, then treated with 100 μg/ml RNase A for 30 min at 37°C, and stained using PI in 0.2% Triton X-100.

Article Title: Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1 2
Article Snippet: Paragraph title: Flow Cytometry Analysis ... Fluorescence signals were measured using a FACScan apparatus (BD Pharmingen) and analyzed by the FlowJo software (Tree Star, Inc, Olten, Switzerland).

Article Title: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
Article Snippet: .. Intensities of fluorescence signals were measured on a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA). .. The percentages of cells in the G0/G1, S, and G2/M phases were determined using FlowJo software (Tree Star).

Article Title: HSP90 Chaperoning in Addition to Phosphoprotein Required for Folding but Not for Supporting Enzymatic Activities of Measles and Nipah Virus L Polymerases
Article Snippet: Paragraph title: Flow cytometry. ... Fluorescence signals were quantified with Accuri C6 (BD Biosciences).

Article Title: Antiphotoaging and Antimelanogenic Effects of Penthorum chinense Pursh Ethanol Extract due to Antioxidant- and Autophagy-Inducing Properties
Article Snippet: .. Fluorescence signals from the cells were measured using a BD FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA) and CellQuest Pro (IVD) software (Becton Dickinson). ..

Article Title: Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis
Article Snippet: .. Fluorescence signals of the beads were acquired by a flow cytometer (FACS Canto II, Becton Dickinson). ..

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: To take protein expression variations into account, flow cytometry was performed contemporaneously with the luciferase assay. .. Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK).

Transfection:

Article Title: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
Article Snippet: Intensities of fluorescence signals were measured on a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA). .. Transfected cells were harvested, double-labeled with Annexin V-FITC and PI apoptosis detection kits (KeyGEN Biotech, Nanjing, China), and analyzed using a FACScan flow cytometry.

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: In each well of a 12-well plate, 1.27×105 293-T cells were seeded and, 24 hours later, transfected with the same mixture of plasmids as were the 96-well plates, except that each well of a 12-well plate was treated with 12.7 times the amount of transfection mixture used for a well of a 96-well plate. .. Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK).

Infection:

Article Title: ER membrane protein complex subunit 6 (EMC6) is a novel tumor suppressor in gastric cancer
Article Snippet: Cell apoptosis assay BGC823 cells were infected with either Ad5-Null or Ad5-EMC6 for 24 to 96 h. Cell apoptosis was detected using an FITC-Annexin V/PI staining detection kit (Beijing Biosea Biotechnology Co., Ltd., China) according to the manufacturer’s instruction. .. Fluorescence signals were detected through a FACSCalibur flow cytometer (BD Biosciences, Franklinlake, NJ, USA) to determine the percentage of apoptotic cells, which included Annexin V+ PI+ double and Annexin V+ single positive cells.

Article Title: HSP90 Chaperoning in Addition to Phosphoprotein Required for Folding but Not for Supporting Enzymatic Activities of Measles and Nipah Virus L Polymerases
Article Snippet: Cells infected with MeV IC323-EGFP were trypsinized, washed, and fixed in 1% paraformaldehyde for 10 min at room temperature. .. Fluorescence signals were quantified with Accuri C6 (BD Biosciences).

Double Staining:

Article Title: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
Article Snippet: Intensities of fluorescence signals were measured on a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA). .. The percentage of cells actively undergoing apoptosis was determined by double staining with Annexin V-fluorescein isothiocyanate (FITC) and PI.

Fluorescence:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: .. Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle. .. FACS enrichment of DNA-carrying beads was carried out by collecting beads from the fluorescent population into a microcentrifuge tube containing 150 μl 1×TE (10 mM Tris–HCl, pH 7.5, 1 mM EDTA).

Article Title: Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes
Article Snippet: .. Fluorescence signals from the peptide-SLA class II complexes was quantified by FACScalibur (BD Bioscience, San Jose, CA, USA) with CellQuest software. ..

Article Title: Engineering modular intracellular protein sensor-actuator devices
Article Snippet: .. Fluorescence signals were then quantified with a flow-cytometer BD LSR-II system (Becton Dickinson) and FACSDiva8 software with the following settings: 640 nm laser and 670/14 nm filter. .. Flow cytometry data were converted from arbitrary units to compensated molecules of equivalent soluble fluorochrome (MESF) using Spherotech #RCP-30- 5A-2.

Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
Article Snippet: .. Finally, the fluorescence signals of DCFH-DA was read at 488 nm excitation and 525 nm emission by the BD FACS Vantage SE Flow Cytometer (BD), and DHE was read at 535 nm excitation and 610 nm emission by a microplate reader (Thermo Fischer). .. ELISA The concentrations of neutrophil elastase (NE) in the serum of human peripheral blood were assayed with an ELISA kit (JYM, 1078, China) according to the manufacturer's instructions.

Article Title: ER membrane protein complex subunit 6 (EMC6) is a novel tumor suppressor in gastric cancer
Article Snippet: .. Fluorescence signals were detected through a FACSCalibur flow cytometer (BD Biosciences, Franklinlake, NJ, USA) to determine the percentage of apoptotic cells, which included Annexin V+ PI+ double and Annexin V+ single positive cells. .. Cell cycle analysis BGC823 cells were infected with either Ad5-Null or Ad5-EMC6 for 24 to 96 h. The cells were fixed with 70% ethanol at −12°C overnight, then treated with 100 μg/ml RNase A for 30 min at 37°C, and stained using PI in 0.2% Triton X-100.

Article Title: Complement receptors regulate lipopolysaccharide-induced T-cell stimulation
Article Snippet: .. Fluorescence signals were determined using a FACScan (Becton Dickinson, San Jose, CA). ..

Article Title: Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1 2
Article Snippet: .. Fluorescence signals were measured using a FACScan apparatus (BD Pharmingen) and analyzed by the FlowJo software (Tree Star, Inc, Olten, Switzerland). ..

Article Title: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
Article Snippet: .. Intensities of fluorescence signals were measured on a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA). .. The percentages of cells in the G0/G1, S, and G2/M phases were determined using FlowJo software (Tree Star).

Article Title: HSP90 Chaperoning in Addition to Phosphoprotein Required for Folding but Not for Supporting Enzymatic Activities of Measles and Nipah Virus L Polymerases
Article Snippet: .. Fluorescence signals were quantified with Accuri C6 (BD Biosciences). .. Monolayers of Vero/ h SLAM, BSR-T7, or BSR-T7- h Slam cells were either infected with MeV at the MOI indicated or transfected with plasmids coding for N, P, M, or Flag/L with JetPrime (Polyplus).

Article Title: Antiphotoaging and Antimelanogenic Effects of Penthorum chinense Pursh Ethanol Extract due to Antioxidant- and Autophagy-Inducing Properties
Article Snippet: .. Fluorescence signals from the cells were measured using a BD FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA) and CellQuest Pro (IVD) software (Becton Dickinson). ..

Article Title: Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis
Article Snippet: .. Fluorescence signals of the beads were acquired by a flow cytometer (FACS Canto II, Becton Dickinson). ..

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: .. Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK). .. Data were analyzed using Flowjo 7.2.5 software (Flowjo, Ashland, OR, USA).

Negative Control:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: A negative control was also prepared, consisting of 15 000 nonreacted DNA Capture Beads labeled with 2 μl streptavidin-conjugated Alexa647 dye (0.2 mg/ml) in 1×AB, in a total volume of 27 μl. .. Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle.

Labeling:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: .. Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle. .. FACS enrichment of DNA-carrying beads was carried out by collecting beads from the fluorescent population into a microcentrifuge tube containing 150 μl 1×TE (10 mM Tris–HCl, pH 7.5, 1 mM EDTA).

Article Title: Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1 2
Article Snippet: Cells were then labeled with a biotin-conjugated monoclonal antibody from BD Pharmingen (anti-B220, clone RA3-6B2) then with PE-conjugated streptavidin. .. Fluorescence signals were measured using a FACScan apparatus (BD Pharmingen) and analyzed by the FlowJo software (Tree Star, Inc, Olten, Switzerland).

Staining:

Article Title: Engineering modular intracellular protein sensor-actuator devices
Article Snippet: Surface expression of HLA-I molecules was determined by staining before fixation with AlexaFluor® 647 mouse anti-human HLA-A, B, C antibody clone W6/32 (Biolegend® #311414, dilution 1:20). .. Fluorescence signals were then quantified with a flow-cytometer BD LSR-II system (Becton Dickinson) and FACSDiva8 software with the following settings: 640 nm laser and 670/14 nm filter.

Article Title: ER membrane protein complex subunit 6 (EMC6) is a novel tumor suppressor in gastric cancer
Article Snippet: Cell apoptosis assay BGC823 cells were infected with either Ad5-Null or Ad5-EMC6 for 24 to 96 h. Cell apoptosis was detected using an FITC-Annexin V/PI staining detection kit (Beijing Biosea Biotechnology Co., Ltd., China) according to the manufacturer’s instruction. .. Fluorescence signals were detected through a FACSCalibur flow cytometer (BD Biosciences, Franklinlake, NJ, USA) to determine the percentage of apoptotic cells, which included Annexin V+ PI+ double and Annexin V+ single positive cells.

Article Title: Complement receptors regulate lipopolysaccharide-induced T-cell stimulation
Article Snippet: After staining cells were fixed with 1% paraformaldehyde. .. Fluorescence signals were determined using a FACScan (Becton Dickinson, San Jose, CA).

Article Title: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
Article Snippet: Fixed cells were then washed in PBS and stained with 0.1 mg/ml propidium iodide (PI, Sigma, St. Louis, MO, USA) containing 1 mg/ml RNase A for 30 min at 37 °C. .. Intensities of fluorescence signals were measured on a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA).

Viability Assay:

Article Title: Antiphotoaging and Antimelanogenic Effects of Penthorum chinense Pursh Ethanol Extract due to Antioxidant- and Autophagy-Inducing Properties
Article Snippet: Paragraph title: 2.3. Cell Viability Assay ... Fluorescence signals from the cells were measured using a BD FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA) and CellQuest Pro (IVD) software (Becton Dickinson).

Software:

Article Title: Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes
Article Snippet: .. Fluorescence signals from the peptide-SLA class II complexes was quantified by FACScalibur (BD Bioscience, San Jose, CA, USA) with CellQuest software. ..

Article Title: Engineering modular intracellular protein sensor-actuator devices
Article Snippet: .. Fluorescence signals were then quantified with a flow-cytometer BD LSR-II system (Becton Dickinson) and FACSDiva8 software with the following settings: 640 nm laser and 670/14 nm filter. .. Flow cytometry data were converted from arbitrary units to compensated molecules of equivalent soluble fluorochrome (MESF) using Spherotech #RCP-30- 5A-2.

Article Title: Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1Differential Influence of Anticancer Treatments and Angiogenesis on the Seric Titer of Autoantibody Used as Tumor and Metastasis Biomarker 1 2
Article Snippet: .. Fluorescence signals were measured using a FACScan apparatus (BD Pharmingen) and analyzed by the FlowJo software (Tree Star, Inc, Olten, Switzerland). ..

Article Title: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
Article Snippet: Intensities of fluorescence signals were measured on a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA). .. The percentages of cells in the G0/G1, S, and G2/M phases were determined using FlowJo software (Tree Star).

Article Title: Antiphotoaging and Antimelanogenic Effects of Penthorum chinense Pursh Ethanol Extract due to Antioxidant- and Autophagy-Inducing Properties
Article Snippet: .. Fluorescence signals from the cells were measured using a BD FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA) and CellQuest Pro (IVD) software (Becton Dickinson). ..

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK). .. Data were analyzed using Flowjo 7.2.5 software (Flowjo, Ashland, OR, USA).

Multiplex Assay:

Article Title: Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis
Article Snippet: Paragraph title: Multiplex flow cytometry ... Fluorescence signals of the beads were acquired by a flow cytometer (FACS Canto II, Becton Dickinson).

Binding Assay:

Article Title: Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes
Article Snippet: Paragraph title: Analysis of peptide binding to SLA class II molecules ... Fluorescence signals from the peptide-SLA class II complexes was quantified by FACScalibur (BD Bioscience, San Jose, CA, USA) with CellQuest software.

Article Title: Antiphotoaging and Antimelanogenic Effects of Penthorum chinense Pursh Ethanol Extract due to Antioxidant- and Autophagy-Inducing Properties
Article Snippet: After 24 h, the cells were harvested, washed twice with PBS, and resuspended in 1x binding buffer. .. Fluorescence signals from the cells were measured using a BD FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA) and CellQuest Pro (IVD) software (Becton Dickinson).

Apoptosis Assay:

Article Title: ER membrane protein complex subunit 6 (EMC6) is a novel tumor suppressor in gastric cancer
Article Snippet: Paragraph title: Cell apoptosis assay ... Fluorescence signals were detected through a FACSCalibur flow cytometer (BD Biosciences, Franklinlake, NJ, USA) to determine the percentage of apoptotic cells, which included Annexin V+ PI+ double and Annexin V+ single positive cells.

Concentration Assay:

Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
Article Snippet: Then, neutrophils were exposed to DMEM medium with different glucose concentrations (5.5, 15, 25, and 35 mM), and stimulation of each concentration lasted for 15 and 30, 60, and 120 min. .. Finally, the fluorescence signals of DCFH-DA was read at 488 nm excitation and 525 nm emission by the BD FACS Vantage SE Flow Cytometer (BD), and DHE was read at 535 nm excitation and 610 nm emission by a microplate reader (Thermo Fischer).

FACS:

Article Title: Flow cytometry for enrichment and titration in massively parallel DNA sequencing
Article Snippet: Paragraph title: Labeling, FACS analysis and sorting ... Fluorescence signals from the SYBR Green and Alexa647 dye labeled beads were detected at 530/30 nm and 660/20 nm, respectively, using a FACSVantage SE flow cytometer (BD Biosciences) with 488 nm and 633 nm excitation lasers, fitted with a 100 μm nozzle.

Article Title: Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes
Article Snippet: Subsequently, the cells were washed twice with the wash buffer and then finally resuspended in 500 μL FACS buffer. .. Fluorescence signals from the peptide-SLA class II complexes was quantified by FACScalibur (BD Bioscience, San Jose, CA, USA) with CellQuest software.

Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
Article Snippet: .. Finally, the fluorescence signals of DCFH-DA was read at 488 nm excitation and 525 nm emission by the BD FACS Vantage SE Flow Cytometer (BD), and DHE was read at 535 nm excitation and 610 nm emission by a microplate reader (Thermo Fischer). .. ELISA The concentrations of neutrophil elastase (NE) in the serum of human peripheral blood were assayed with an ELISA kit (JYM, 1078, China) according to the manufacturer's instructions.

Article Title: Complement receptors regulate lipopolysaccharide-induced T-cell stimulation
Article Snippet: Paragraph title: FACS analysis ... Fluorescence signals were determined using a FACScan (Becton Dickinson, San Jose, CA).

Article Title: Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis
Article Snippet: .. Fluorescence signals of the beads were acquired by a flow cytometer (FACS Canto II, Becton Dickinson). ..

Article Title: CD317/tetherin is an organiser of membrane microdomains
Article Snippet: .. Fluorescence signals were measured using a FACS CantoII-F60 machine (BD Biosciences, Oxford, UK). .. Data were analyzed using Flowjo 7.2.5 software (Flowjo, Ashland, OR, USA).

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    Becton Dickinson gfp fluorescence signal
    Flow <t>cytometry</t> analysis (histograms) of the GFPMTT5 strain exposed to different Cd 2+ concentrations (89 nM – 15 µM). ( a ) 2h or ( b ) 24h treatment. <t>GFP</t> fluorescence was quantified in arbitrary units (au). Continuous line represents the %
    Gfp Fluorescence Signal, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp fluorescence signal/product/Becton Dickinson
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    Becton Dickinson rhodamine 123 fluorescent signal
    Left part of (A) showed the results of <t>Rhodamine</t> 123 staining detected by a flow cytometer. Columns on the right part indicated the mean fluorescent intensity (MFI) of Rhodamine 123 staining. On the left part of (B), the upper panel showed the captured
    Rhodamine 123 Fluorescent Signal, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson fluorescent ghrelin binding signals
    Fluorescent <t>ghrelin</t> binding in the fixed hippocampal slice culture. a. FITC-conjugated acyl-ghrelin (F302)-binding (in green) in the hilus and CA1 with DAPI staining (in blue). b. F302 binding in CA3 with DAPI staining. c. F302 and DAPI in the hilus. d. F302 and DAPI in CA1. e, f, and g. F-ghrelin binding in small punctate structures on the soma and proximal processes. h. FITC-conjugated non-acyl-ghrelin (F203)-binding. i. Competitive binding of F302 with “cold” acyl-ghrelin in control (F302 alone) (n=10 rats) and with the ratios of F/C = 1/1 (10 rats), 1/10 (10 rats), 1/50 (10 rats), and 1/100 (10 rats). Inset in i: Quantification of F302 signals as red ROI. i1. F302 binding in control live slice. i2. F/C=1/50 in live slices. i3 and i5. F302 binding in control fixed slices. i4 and i6. F/C=1/50 in fixed slices. Calibrations: 500 μm in a (shared with b), 10 μm in c (shared with d), 15 μm in e, 10 μm in f, 500 μm in i2 (shared with i1), 500 μm in i4 (shared with i3), and 10 μm in i6 (shared with i5). *p
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    Image Search Results


    Flow cytometry analysis (histograms) of the GFPMTT5 strain exposed to different Cd 2+ concentrations (89 nM – 15 µM). ( a ) 2h or ( b ) 24h treatment. GFP fluorescence was quantified in arbitrary units (au). Continuous line represents the %

    Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

    Article Title: Functional GFP-metallothionein fusion protein from Tetrahymena thermophila: a potential whole-cell biosensor for monitoring heavy metal pollution and a cell model to study metallothionein overproduction effects

    doi: 10.1007/s10534-014-9704-0

    Figure Lengend Snippet: Flow cytometry analysis (histograms) of the GFPMTT5 strain exposed to different Cd 2+ concentrations (89 nM – 15 µM). ( a ) 2h or ( b ) 24h treatment. GFP fluorescence was quantified in arbitrary units (au). Continuous line represents the %

    Article Snippet: To quantify the GFP-fluorescence signal, flow cytometry was performed on a FACScaliburg flow cytometer (Becton Dickinson) equipped with Cell Quest software.

    Techniques: Flow Cytometry, Cytometry, Fluorescence

    Smg6-deficient ESCs fail to differentiate in vitro and in vivo A In vitro culture of control (+/Δ) and Smg6 Δ/Δ (Δ/Δ) blastocysts. Note that there is no ICMs outgrowth of Smg6 Δ/Δ blastocysts on day 5 of culture in contrast to the controls. B Morphology of control (F/F) and Smg6 Δ/Δ ESCs. C Proliferation curve of control (F/F) and Smg6 Δ/Δ ESCs. D Cell death analysis of control (F/F) and Smg6 Δ/Δ ESCs by FACS after Annexin-V staining ( n = 3 for each genotype). E H E staining of control and Smg6 Δ/Δ ESCs derived EBs on day 8. Enlarged panels (right) show that control EBs contained differentiated connective tissues and epithelial-like structures and Smg6 Δ/Δ EBs contained undifferentiated cells. F Immunostaining and quantification (top and lower left) and Western blotting (lower right) of Oct4 expression in control and Smg6 Δ/Δ ESCs derived EBs on day 8. β-Tubulin was used as a loading control. n , the total number of cells counted from five controls and six Smg6 Δ/Δ EBs. G Immunostaining of ectoderm marker Nestin, endoderm marker β-catenin, and mesoderm marker α-SMA on day 8 of control F/F and Δ/Δ EB samples. H ESC differentiation upon DMSO and RA treatment. Note that Smg6 Δ/Δ ESCs continued to maintain ES-like structure, while control ESCs differentiated into different cell types (fibroblast-like cells by DMSO induction; endothelial cells after RA treatment) on day 5 after plating onto gelatin-coated dishes. The morphology of the EBs before plating was shown (day 0). I Immunostaining of Oct4 expression in control and Smg6 Δ/Δ ESCs derived differentiation cultures treated for DMSO or RA on day 8. The frequency of Oct4 + cells was summarized in low panels. n , the total number of cells used for the quantification. At least three differentiation cultures were used. J Chimerism assay of the contribution of GFP-labeled control and Smg6 Δ/Δ ESC derivatives to different mouse tissues (E12.5 embryos are analyzed). Note the absence of GFP-labeled Smg6 Δ/Δ ESC derivatives within chimeras. Representative images of the brain (ectoderm), liver (endoderm), and skin connective tissues (mesoderm) are shown. Data information: The error bars represent the SEM. Unpaired Student's t -test was used. *** P

    Journal: The EMBO Journal

    Article Title: Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay

    doi: 10.15252/embj.201489947

    Figure Lengend Snippet: Smg6-deficient ESCs fail to differentiate in vitro and in vivo A In vitro culture of control (+/Δ) and Smg6 Δ/Δ (Δ/Δ) blastocysts. Note that there is no ICMs outgrowth of Smg6 Δ/Δ blastocysts on day 5 of culture in contrast to the controls. B Morphology of control (F/F) and Smg6 Δ/Δ ESCs. C Proliferation curve of control (F/F) and Smg6 Δ/Δ ESCs. D Cell death analysis of control (F/F) and Smg6 Δ/Δ ESCs by FACS after Annexin-V staining ( n = 3 for each genotype). E H E staining of control and Smg6 Δ/Δ ESCs derived EBs on day 8. Enlarged panels (right) show that control EBs contained differentiated connective tissues and epithelial-like structures and Smg6 Δ/Δ EBs contained undifferentiated cells. F Immunostaining and quantification (top and lower left) and Western blotting (lower right) of Oct4 expression in control and Smg6 Δ/Δ ESCs derived EBs on day 8. β-Tubulin was used as a loading control. n , the total number of cells counted from five controls and six Smg6 Δ/Δ EBs. G Immunostaining of ectoderm marker Nestin, endoderm marker β-catenin, and mesoderm marker α-SMA on day 8 of control F/F and Δ/Δ EB samples. H ESC differentiation upon DMSO and RA treatment. Note that Smg6 Δ/Δ ESCs continued to maintain ES-like structure, while control ESCs differentiated into different cell types (fibroblast-like cells by DMSO induction; endothelial cells after RA treatment) on day 5 after plating onto gelatin-coated dishes. The morphology of the EBs before plating was shown (day 0). I Immunostaining of Oct4 expression in control and Smg6 Δ/Δ ESCs derived differentiation cultures treated for DMSO or RA on day 8. The frequency of Oct4 + cells was summarized in low panels. n , the total number of cells used for the quantification. At least three differentiation cultures were used. J Chimerism assay of the contribution of GFP-labeled control and Smg6 Δ/Δ ESC derivatives to different mouse tissues (E12.5 embryos are analyzed). Note the absence of GFP-labeled Smg6 Δ/Δ ESC derivatives within chimeras. Representative images of the brain (ectoderm), liver (endoderm), and skin connective tissues (mesoderm) are shown. Data information: The error bars represent the SEM. Unpaired Student's t -test was used. *** P

    Article Snippet: For the positive control, ESCs with the NMD reporter were pretreated with 100 μg/ml CHX (Sigma-Aldrich) for 3 h. NMD efficiency was determined by GFP fluorescence signal intensity by FACS analysis with a FACScanto flow cytometer equipped with FACSDiva software (Becton Dickson, Mountain View, CA, USA).

    Techniques: In Vitro, In Vivo, FACS, Staining, Derivative Assay, Immunostaining, Western Blot, Expressing, Marker, Labeling

    Smg6 is required for telomere maintenance and NMD in ESCs A Telomere FISH analysis of control (F/F) and Smg6 Δ/Δ (Δ/Δ) ESC metaphases. Representative images (up) and quantification of chromosomes lacking telomere signals (telomere-signal-free ends) (lower panel) are shown. n , the number of metaphases analyzed. B qRT–PCR analysis of NMD target transcripts in control and Smg6 Δ/Δ ESCs. The expression levels of the NMD target genes were normalized to β-actin. The data are from three independent biological samples. C RT–PCR analysis of exon inclusion generated PTC-containing (*PTC+) isoforms in control and Smg6 Δ/Δ ESCs. D RT–PCR analysis of exon exclusion generated PTC-containing (*PTC+) isoforms in control and Smg6 Δ/Δ ESCs. E Smg6 Δ/Δ ESCs are NMD defective. ESCs were transfected with the NMD reporter (upper panel) and analyzed in triplicate by FACS. The GFP signal intensity determines the NMD activity. CHX was used to inhibit NMD. The data represent one of two independent ES clones of each genotype. Data information: The error bars represent the SEM. Unpaired Student's t -test was used. n.s., not significant, P > 0.05; * P

    Journal: The EMBO Journal

    Article Title: Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay

    doi: 10.15252/embj.201489947

    Figure Lengend Snippet: Smg6 is required for telomere maintenance and NMD in ESCs A Telomere FISH analysis of control (F/F) and Smg6 Δ/Δ (Δ/Δ) ESC metaphases. Representative images (up) and quantification of chromosomes lacking telomere signals (telomere-signal-free ends) (lower panel) are shown. n , the number of metaphases analyzed. B qRT–PCR analysis of NMD target transcripts in control and Smg6 Δ/Δ ESCs. The expression levels of the NMD target genes were normalized to β-actin. The data are from three independent biological samples. C RT–PCR analysis of exon inclusion generated PTC-containing (*PTC+) isoforms in control and Smg6 Δ/Δ ESCs. D RT–PCR analysis of exon exclusion generated PTC-containing (*PTC+) isoforms in control and Smg6 Δ/Δ ESCs. E Smg6 Δ/Δ ESCs are NMD defective. ESCs were transfected with the NMD reporter (upper panel) and analyzed in triplicate by FACS. The GFP signal intensity determines the NMD activity. CHX was used to inhibit NMD. The data represent one of two independent ES clones of each genotype. Data information: The error bars represent the SEM. Unpaired Student's t -test was used. n.s., not significant, P > 0.05; * P

    Article Snippet: For the positive control, ESCs with the NMD reporter were pretreated with 100 μg/ml CHX (Sigma-Aldrich) for 3 h. NMD efficiency was determined by GFP fluorescence signal intensity by FACS analysis with a FACScanto flow cytometer equipped with FACSDiva software (Becton Dickson, Mountain View, CA, USA).

    Techniques: Fluorescence In Situ Hybridization, Quantitative RT-PCR, Expressing, Reverse Transcription Polymerase Chain Reaction, Generated, Transfection, FACS, Activity Assay, Clone Assay

    Left part of (A) showed the results of Rhodamine 123 staining detected by a flow cytometer. Columns on the right part indicated the mean fluorescent intensity (MFI) of Rhodamine 123 staining. On the left part of (B), the upper panel showed the captured

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Emodin induces apoptosis of human osteosarcoma cells via mitochondria- and endoplasmic reticulum stress-related pathways

    doi:

    Figure Lengend Snippet: Left part of (A) showed the results of Rhodamine 123 staining detected by a flow cytometer. Columns on the right part indicated the mean fluorescent intensity (MFI) of Rhodamine 123 staining. On the left part of (B), the upper panel showed the captured

    Article Snippet: Rhodamine 123 fluorescent signal was detected by a FACS flow cytometer (BD) at 529 nm.

    Techniques: Staining, Flow Cytometry, Cytometry

    Fluorescent ghrelin binding in the fixed hippocampal slice culture. a. FITC-conjugated acyl-ghrelin (F302)-binding (in green) in the hilus and CA1 with DAPI staining (in blue). b. F302 binding in CA3 with DAPI staining. c. F302 and DAPI in the hilus. d. F302 and DAPI in CA1. e, f, and g. F-ghrelin binding in small punctate structures on the soma and proximal processes. h. FITC-conjugated non-acyl-ghrelin (F203)-binding. i. Competitive binding of F302 with “cold” acyl-ghrelin in control (F302 alone) (n=10 rats) and with the ratios of F/C = 1/1 (10 rats), 1/10 (10 rats), 1/50 (10 rats), and 1/100 (10 rats). Inset in i: Quantification of F302 signals as red ROI. i1. F302 binding in control live slice. i2. F/C=1/50 in live slices. i3 and i5. F302 binding in control fixed slices. i4 and i6. F/C=1/50 in fixed slices. Calibrations: 500 μm in a (shared with b), 10 μm in c (shared with d), 15 μm in e, 10 μm in f, 500 μm in i2 (shared with i1), 500 μm in i4 (shared with i3), and 10 μm in i6 (shared with i5). *p

    Journal: Journal of neurochemistry

    Article Title: Endogenous ghrelin-O-acyltransferase (GOAT) acylates local ghrelin in the hippocampus

    doi: 10.1111/jnc.14244

    Figure Lengend Snippet: Fluorescent ghrelin binding in the fixed hippocampal slice culture. a. FITC-conjugated acyl-ghrelin (F302)-binding (in green) in the hilus and CA1 with DAPI staining (in blue). b. F302 binding in CA3 with DAPI staining. c. F302 and DAPI in the hilus. d. F302 and DAPI in CA1. e, f, and g. F-ghrelin binding in small punctate structures on the soma and proximal processes. h. FITC-conjugated non-acyl-ghrelin (F203)-binding. i. Competitive binding of F302 with “cold” acyl-ghrelin in control (F302 alone) (n=10 rats) and with the ratios of F/C = 1/1 (10 rats), 1/10 (10 rats), 1/50 (10 rats), and 1/100 (10 rats). Inset in i: Quantification of F302 signals as red ROI. i1. F302 binding in control live slice. i2. F/C=1/50 in live slices. i3 and i5. F302 binding in control fixed slices. i4 and i6. F/C=1/50 in fixed slices. Calibrations: 500 μm in a (shared with b), 10 μm in c (shared with d), 15 μm in e, 10 μm in f, 500 μm in i2 (shared with i1), 500 μm in i4 (shared with i3), and 10 μm in i6 (shared with i5). *p

    Article Snippet: Fluorescent ghrelin binding signals and immunoreactive products were identified and selected as a region of interest (ROI) using an auto-segmentation tool under the Triangle logic provided by the IPLab image analysis software (Scanalytics/BD Bioscience, San Jose, CA).

    Techniques: Binding Assay, Staining

    Non-octanoylated ghrelin (F203) binding in live hippocampal slice culture. a. Overnight incubation of live rat hippocampal slice in F302. b. Overnight incubation of live rat hippocampal slice in F203. c. Overnight incubation of GHSR1a KO live slice in F302. d. Overnight incubation of GHSR1a KO live slice in F203. e. Overnight incubation of fixed rat slice culture in F302. f. Overnight incubation of fixed rat slice culture in F203. g. Overnight incubation of live rat hippocampal slice in GOAT inhibitor with F302. h. Overnight incubation of live rat hippocampal slice in GOAT inhibitor with F203. i. Summary of results: F302 (n=15 rats), F203 (n=15 rats), F302 in GHSR1a KO (n=7 mice), F203 in GHSR1a KO (n=7 mice), F203 in fixed slices (n=7 rats), and F203 in GOAT inhibitor (n=7 rats). Inset in i depicts FITC signals in red ROIs. Calibrations: 300 μm in a (shared with b, c, and d). 400 μm in e (shared with f, g, and h). * p

    Journal: Journal of neurochemistry

    Article Title: Endogenous ghrelin-O-acyltransferase (GOAT) acylates local ghrelin in the hippocampus

    doi: 10.1111/jnc.14244

    Figure Lengend Snippet: Non-octanoylated ghrelin (F203) binding in live hippocampal slice culture. a. Overnight incubation of live rat hippocampal slice in F302. b. Overnight incubation of live rat hippocampal slice in F203. c. Overnight incubation of GHSR1a KO live slice in F302. d. Overnight incubation of GHSR1a KO live slice in F203. e. Overnight incubation of fixed rat slice culture in F302. f. Overnight incubation of fixed rat slice culture in F203. g. Overnight incubation of live rat hippocampal slice in GOAT inhibitor with F302. h. Overnight incubation of live rat hippocampal slice in GOAT inhibitor with F203. i. Summary of results: F302 (n=15 rats), F203 (n=15 rats), F302 in GHSR1a KO (n=7 mice), F203 in GHSR1a KO (n=7 mice), F203 in fixed slices (n=7 rats), and F203 in GOAT inhibitor (n=7 rats). Inset in i depicts FITC signals in red ROIs. Calibrations: 300 μm in a (shared with b, c, and d). 400 μm in e (shared with f, g, and h). * p

    Article Snippet: Fluorescent ghrelin binding signals and immunoreactive products were identified and selected as a region of interest (ROI) using an auto-segmentation tool under the Triangle logic provided by the IPLab image analysis software (Scanalytics/BD Bioscience, San Jose, CA).

    Techniques: Binding Assay, Incubation, Mouse Assay