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Carl Zeiss fluorescence signal
Fluorescence Signal, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 194 article reviews
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fluorescence signal - by Bioz Stars, 2020-04
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Related Articles

Immunocytochemistry:

Article Title: Luminal Alkalinization Attenuates Proteinuria-Induced Oxidative Damage in Proximal Tubular Cells
Article Snippet: Paragraph title: Immunocytochemistry ... The fluorescence signal was observed with a LSM510 confocal imaging system (Carl Zeiss).

Mass Spectrometry:

Article Title: Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation
Article Snippet: The experimental setup used to record fluorescence signal included an upright Axio Scope.A1 microscope (Zeiss, Thornwood, NY) fitted with a 625 mW blue LED light source (470 nm center wavelength, MBLED, Thorlabs, Newton, NJ). .. Fluorescence was detected using a Photometrics CoolSNAP HQ2 (Tucson, AZ) cooled CCD camera at 1.67 Hz with a 600 ms exposure time.

Stable Transfection:

Article Title: Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy
Article Snippet: Preparation of transgenic line carrying PLDα1-YFP and mRFP-TUB6 Arabidopsis pldα1-2 stably expressing proPLDα1::PLDα1:YFP in T2 generation were crossed with Col-0 plants stably expressing pUBQ1:mRFP::TUB6 kindly provided by Geoffrey O. Wasteneys (Ambrose et al., ). .. F1 generation plants with PLDα1-YFP and mRFP-TUB6 expression were selected based on fluorescence signal in the epifluorescence microscope (Axio Imager.M2, Carl Zeiss, Germany).

Construct:

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: The resulting construct was used to transform Arabidopsis protoplasts to determine the subcellular localization of the CLD1-GFP fusion protein as described ( ; ). .. The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set.

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: Standard fly work and microscopy Mutations and deficiencies (Table ) were kept over balancers harbouring GFP or YFP constructs expressed under the control of either Krüppel or Deformed . .. Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope.

Incubation:

Article Title: Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice
Article Snippet: After the incubation period, cells were washed three times with ice-cold PBS. .. The fluorescence signal of the cells was recorded using an Axiovert 200M fluorescence microscope (Carl Zeiss MicroImaging, Inc., Thornwood, NY) equipped with a GFP filter set (Exciter, HQ 475/20 nm; Emitter, HQ 540/30 nm).

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope. .. For cuticle preparations, larvae (n > 50) were deposited on a glass slide in Hoyer’s medium covered by a coverslip and incubated at 65 °C or 80 °C overnight.

Article Title: Luminal Alkalinization Attenuates Proteinuria-Induced Oxidative Damage in Proximal Tubular Cells
Article Snippet: Cells were then incubated with a 1:500 diluted polyclonal anti-aquaporin 1 antibody (Abcam, ab65837) overnight at 4°C, and then with Alexa Fluor 488-conjugated anti-rabbit IgG antibody (Invitrogen) for 1 hour at room temperature. .. The fluorescence signal was observed with a LSM510 confocal imaging system (Carl Zeiss).

Article Title: Recycling of IRAP from the plasma membrane back to the insulin-responsive compartment requires the Q-SNARE syntaxin 6 but not the GGA clathrin adaptors
Article Snippet: Cells were then cooled on ice and incubated with the anti-TfR antibody (2 ng/ml) for 60 minutes. .. Quantification of the fluorescence signal at the cell surface versus the total-cell fluorescence was performed using the Zeiss LSM software package.

Expressing:

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: This allows identification of homozygous mutant embryos, which lack any GFP/YFP expression. .. Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope.

Article Title: Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy
Article Snippet: .. F1 generation plants with PLDα1-YFP and mRFP-TUB6 expression were selected based on fluorescence signal in the epifluorescence microscope (Axio Imager.M2, Carl Zeiss, Germany). ..

Transformation Assay:

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: For the GFP control, pMDC83 was used for the transient transformation. .. The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set.

Transfection:

Article Title: Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
Article Snippet: .. After 48 h of transfection, the fluorescence signal was acquired using a Zeiss LSM 310 confocal microscope and the appropriate filters set. .. For RNA transfection of S2 cells, RNA was prepared with the Ampliscribe mRNA transcription kit (Biozym Diagnostik GmbH) as described above.

Cell Culture:

Article Title: Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice
Article Snippet: For fluorescence microscopy studies, U87MG cells (1 × 105 ) were cultured on 35 mm MatTek glass bottom culture dishes (cat. no.: P35G-0-14-C, Ashland, MA). .. The fluorescence signal of the cells was recorded using an Axiovert 200M fluorescence microscope (Carl Zeiss MicroImaging, Inc., Thornwood, NY) equipped with a GFP filter set (Exciter, HQ 475/20 nm; Emitter, HQ 540/30 nm).

Article Title: Diminished Superoxide Generation Is Associated With Respiratory Chain Dysfunction and Changes in the Mitochondrial Proteome of Sensory Neurons From Diabetic Rats
Article Snippet: .. Cultured DRG neurons were loaded with 3.0 nmol/l tetramethyl rhodamine methyl ester (TMRM; Molecular Probes, Eugene, OR) for 1 h and the fluorescence signal in the axons detected with a Carl Zeiss LSM510 confocal inverted microscope (X100 objective; excitation at 540 nm and emission > 560 nm). .. The TMRM was used in subquench mode—in which decreased fluorescence intensity indicates reduced mitochondrial inner membrane potential ( ).

Light Microscopy:

Article Title: Embryology of two mycoheterotrophic orchid species, Gastrodia elata and Gastrodia nantoensis: ovule and embryo development
Article Snippet: Paragraph title: Light microscopy and histochemical observations ... The fluorescence signal was examined using an epifluorescence microscope (Axioskop 2, Carl Zeiss AG) equipped with the Zeiss filter set 15 (546/12 nm excitation filter and 590 emission barrier filter).

Imaging:

Article Title: Luminal Alkalinization Attenuates Proteinuria-Induced Oxidative Damage in Proximal Tubular Cells
Article Snippet: .. The fluorescence signal was observed with a LSM510 confocal imaging system (Carl Zeiss). ..

Inverted Microscopy:

Article Title: Diminished Superoxide Generation Is Associated With Respiratory Chain Dysfunction and Changes in the Mitochondrial Proteome of Sensory Neurons From Diabetic Rats
Article Snippet: .. Cultured DRG neurons were loaded with 3.0 nmol/l tetramethyl rhodamine methyl ester (TMRM; Molecular Probes, Eugene, OR) for 1 h and the fluorescence signal in the axons detected with a Carl Zeiss LSM510 confocal inverted microscope (X100 objective; excitation at 540 nm and emission > 560 nm). .. The TMRM was used in subquench mode—in which decreased fluorescence intensity indicates reduced mitochondrial inner membrane potential ( ).

Sequencing:

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: CLD1 cDNA was ligated into the vector pCR8/GW/TOPO for sequencing confirmation and subsequent subcloning into pMDC83 for generating a chimeric gene with C-terminal in-frame fusion to GFP as described ( ). .. The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set.

Injection:

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: .. Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope. .. For cuticle preparations, larvae (n > 50) were deposited on a glass slide in Hoyer’s medium covered by a coverslip and incubated at 65 °C or 80 °C overnight.

Article Title: Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation
Article Snippet: For each experiment, 400–500 nL of protein solution was loaded into the injection reservoir using a 5 µL syringe (Hamilton, Reno, NV), which caused filling of the nanochannels due to capillary action. .. The experimental setup used to record fluorescence signal included an upright Axio Scope.A1 microscope (Zeiss, Thornwood, NY) fitted with a 625 mW blue LED light source (470 nm center wavelength, MBLED, Thorlabs, Newton, NJ).

Article Title: Diminished Superoxide Generation Is Associated With Respiratory Chain Dysfunction and Changes in the Mitochondrial Proteome of Sensory Neurons From Diabetic Rats
Article Snippet: Cultured DRG neurons were loaded with 3.0 nmol/l tetramethyl rhodamine methyl ester (TMRM; Molecular Probes, Eugene, OR) for 1 h and the fluorescence signal in the axons detected with a Carl Zeiss LSM510 confocal inverted microscope (X100 objective; excitation at 540 nm and emission > 560 nm). .. Antimycin A and oligomycin (Sigma) were injected into the culture media to a final concentration of 10 μmol/l and 1 μmol/l, respectively, at 1 min after baseline fluorescence measurements.

Fluorescence:

Article Title: Regulation of Fat Storage and Reproduction by Krüppel-Like Transcription Factor KLF3 and Fat-Associated Genes in Caenorhabditis elegans
Article Snippet: .. After 10 min or 15 min of feeding, the animals were observed for fluorescence signal under a Zeiss Axioplan 2 fluorescence microscope, and the images of fat accumulation were recorded for a comparison of fat deposits between wild type and klf-3 ( ok1975 ) mutants. ..

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: .. The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set. .. Fractionation of chloroplastic compartments was performed as described ( ).

Article Title: Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
Article Snippet: .. After 48 h of transfection, the fluorescence signal was acquired using a Zeiss LSM 310 confocal microscope and the appropriate filters set. .. For RNA transfection of S2 cells, RNA was prepared with the Ampliscribe mRNA transcription kit (Biozym Diagnostik GmbH) as described above.

Article Title: Embryology of two mycoheterotrophic orchid species, Gastrodia elata and Gastrodia nantoensis: ovule and embryo development
Article Snippet: .. The fluorescence signal was examined using an epifluorescence microscope (Axioskop 2, Carl Zeiss AG) equipped with the Zeiss filter set 15 (546/12 nm excitation filter and 590 emission barrier filter). .. These sections were viewed and the images were captured digitally using a CCD camera attached to the light microscope.

Article Title: Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice
Article Snippet: .. The fluorescence signal of the cells was recorded using an Axiovert 200M fluorescence microscope (Carl Zeiss MicroImaging, Inc., Thornwood, NY) equipped with a GFP filter set (Exciter, HQ 475/20 nm; Emitter, HQ 540/30 nm). .. An AttoArc HBO 100W microscopic illuminator was used as a light source for fluorescence excitation.

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: .. Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope. .. For cuticle preparations, larvae (n > 50) were deposited on a glass slide in Hoyer’s medium covered by a coverslip and incubated at 65 °C or 80 °C overnight.

Article Title: Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation
Article Snippet: .. The experimental setup used to record fluorescence signal included an upright Axio Scope.A1 microscope (Zeiss, Thornwood, NY) fitted with a 625 mW blue LED light source (470 nm center wavelength, MBLED, Thorlabs, Newton, NJ). .. A brightline filter cube (FITC-LP01-Clinical-OMF, Semrock, Rochester, NY) consisting of a longpass dichroic mirror with a cutoff wavelength of 515 nm was used to separate excitation and emission signal.

Article Title: Fine Particulate Matter Leads to Unfolded Protein Response and Shortened Lifespan by Inducing Oxidative Stress in C. elegans
Article Snippet: .. The fluorescence signal was observed under a fluorescence microscope (Zeiss, Axio Observer Z1, Germany) ( excitation wavelength: 480 nm; emission wavelength: 510 nm), and the intensities of the relative fluorescent units (RFU) in the intestine were measured and quantified by the ImageJ program (NIH, Bethesda, MD). .. Measurement of Oxidative Stress Markers After exposure, worms were collected and washed with K buffer, then precooled lysis buffer (pH 7.4, 0.01 mol/L Tris-HCl, 0.0001 mol/L EDTA-2Na, 0.01 mol/L saccharose, and 0.8% NaCl) was added and the mixture was transferred to a glass homogenizer for homogenizing.

Article Title: Diminished Superoxide Generation Is Associated With Respiratory Chain Dysfunction and Changes in the Mitochondrial Proteome of Sensory Neurons From Diabetic Rats
Article Snippet: .. Cultured DRG neurons were loaded with 3.0 nmol/l tetramethyl rhodamine methyl ester (TMRM; Molecular Probes, Eugene, OR) for 1 h and the fluorescence signal in the axons detected with a Carl Zeiss LSM510 confocal inverted microscope (X100 objective; excitation at 540 nm and emission > 560 nm). .. The TMRM was used in subquench mode—in which decreased fluorescence intensity indicates reduced mitochondrial inner membrane potential ( ).

Article Title: Luminal Alkalinization Attenuates Proteinuria-Induced Oxidative Damage in Proximal Tubular Cells
Article Snippet: .. The fluorescence signal was observed with a LSM510 confocal imaging system (Carl Zeiss). ..

Article Title: Recycling of IRAP from the plasma membrane back to the insulin-responsive compartment requires the Q-SNARE syntaxin 6 but not the GGA clathrin adaptors
Article Snippet: .. Quantification of the fluorescence signal at the cell surface versus the total-cell fluorescence was performed using the Zeiss LSM software package. ..

Article Title: Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy
Article Snippet: .. F1 generation plants with PLDα1-YFP and mRFP-TUB6 expression were selected based on fluorescence signal in the epifluorescence microscope (Axio Imager.M2, Carl Zeiss, Germany). ..

Mutagenesis:

Article Title: Regulation of Fat Storage and Reproduction by Krüppel-Like Transcription Factor KLF3 and Fat-Associated Genes in Caenorhabditis elegans
Article Snippet: The klf-3 ( ok1975 ) mutant animals and, for comparison, the wild-type animals were fed equal amounts of OP50 incorporated into BODIPY dye. .. After 10 min or 15 min of feeding, the animals were observed for fluorescence signal under a Zeiss Axioplan 2 fluorescence microscope, and the images of fat accumulation were recorded for a comparison of fat deposits between wild type and klf-3 ( ok1975 ) mutants.

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: This allows identification of homozygous mutant embryos, which lack any GFP/YFP expression. .. Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope.

Isolation:

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set. .. Briefly, leaves of 4-week-old plants were collected, and chloroplasts were isolated and partitioned into envelope/stroma and thylakoid fractions.

Subcloning:

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: CLD1 cDNA was ligated into the vector pCR8/GW/TOPO for sequencing confirmation and subsequent subcloning into pMDC83 for generating a chimeric gene with C-terminal in-frame fusion to GFP as described ( ). .. The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set.

Flow Cytometry:

Article Title: Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation
Article Snippet: Solvent evaporation from the channel ends after capillary flow maintained some additional flow. .. The experimental setup used to record fluorescence signal included an upright Axio Scope.A1 microscope (Zeiss, Thornwood, NY) fitted with a 625 mW blue LED light source (470 nm center wavelength, MBLED, Thorlabs, Newton, NJ).

Microscopy:

Article Title: Regulation of Fat Storage and Reproduction by Krüppel-Like Transcription Factor KLF3 and Fat-Associated Genes in Caenorhabditis elegans
Article Snippet: .. After 10 min or 15 min of feeding, the animals were observed for fluorescence signal under a Zeiss Axioplan 2 fluorescence microscope, and the images of fat accumulation were recorded for a comparison of fat deposits between wild type and klf-3 ( ok1975 ) mutants. ..

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: .. The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set. .. Fractionation of chloroplastic compartments was performed as described ( ).

Article Title: Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
Article Snippet: .. After 48 h of transfection, the fluorescence signal was acquired using a Zeiss LSM 310 confocal microscope and the appropriate filters set. .. For RNA transfection of S2 cells, RNA was prepared with the Ampliscribe mRNA transcription kit (Biozym Diagnostik GmbH) as described above.

Article Title: Embryology of two mycoheterotrophic orchid species, Gastrodia elata and Gastrodia nantoensis: ovule and embryo development
Article Snippet: .. The fluorescence signal was examined using an epifluorescence microscope (Axioskop 2, Carl Zeiss AG) equipped with the Zeiss filter set 15 (546/12 nm excitation filter and 590 emission barrier filter). .. These sections were viewed and the images were captured digitally using a CCD camera attached to the light microscope.

Article Title: Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice
Article Snippet: .. The fluorescence signal of the cells was recorded using an Axiovert 200M fluorescence microscope (Carl Zeiss MicroImaging, Inc., Thornwood, NY) equipped with a GFP filter set (Exciter, HQ 475/20 nm; Emitter, HQ 540/30 nm). .. An AttoArc HBO 100W microscopic illuminator was used as a light source for fluorescence excitation.

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: .. Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope. .. For cuticle preparations, larvae (n > 50) were deposited on a glass slide in Hoyer’s medium covered by a coverslip and incubated at 65 °C or 80 °C overnight.

Article Title: Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation
Article Snippet: .. The experimental setup used to record fluorescence signal included an upright Axio Scope.A1 microscope (Zeiss, Thornwood, NY) fitted with a 625 mW blue LED light source (470 nm center wavelength, MBLED, Thorlabs, Newton, NJ). .. A brightline filter cube (FITC-LP01-Clinical-OMF, Semrock, Rochester, NY) consisting of a longpass dichroic mirror with a cutoff wavelength of 515 nm was used to separate excitation and emission signal.

Article Title: Fine Particulate Matter Leads to Unfolded Protein Response and Shortened Lifespan by Inducing Oxidative Stress in C. elegans
Article Snippet: .. The fluorescence signal was observed under a fluorescence microscope (Zeiss, Axio Observer Z1, Germany) ( excitation wavelength: 480 nm; emission wavelength: 510 nm), and the intensities of the relative fluorescent units (RFU) in the intestine were measured and quantified by the ImageJ program (NIH, Bethesda, MD). .. Measurement of Oxidative Stress Markers After exposure, worms were collected and washed with K buffer, then precooled lysis buffer (pH 7.4, 0.01 mol/L Tris-HCl, 0.0001 mol/L EDTA-2Na, 0.01 mol/L saccharose, and 0.8% NaCl) was added and the mixture was transferred to a glass homogenizer for homogenizing.

Article Title: Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy
Article Snippet: .. F1 generation plants with PLDα1-YFP and mRFP-TUB6 expression were selected based on fluorescence signal in the epifluorescence microscope (Axio Imager.M2, Carl Zeiss, Germany). ..

Transgenic Assay:

Article Title: Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy
Article Snippet: Paragraph title: Preparation of transgenic line carrying PLDα1-YFP and mRFP-TUB6 ... F1 generation plants with PLDα1-YFP and mRFP-TUB6 expression were selected based on fluorescence signal in the epifluorescence microscope (Axio Imager.M2, Carl Zeiss, Germany).

Labeling:

Article Title: Recycling of IRAP from the plasma membrane back to the insulin-responsive compartment requires the Q-SNARE syntaxin 6 but not the GGA clathrin adaptors
Article Snippet: Cells were then fixed in 4% paraformaldehyde/0.2% TX-100 for 10 minutes, blocked with 5% donkey serum and 1% BSA for 30 minutes and labeled with Texas-Red-conjugated secondary antibody (Jackson ImmunoResearch Labs) for 30 minutes at 37°C. .. Quantification of the fluorescence signal at the cell surface versus the total-cell fluorescence was performed using the Zeiss LSM software package.

Plasmid Preparation:

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: CLD1 cDNA was ligated into the vector pCR8/GW/TOPO for sequencing confirmation and subsequent subcloning into pMDC83 for generating a chimeric gene with C-terminal in-frame fusion to GFP as described ( ). .. The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set.

Article Title: Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
Article Snippet: Drosophila Schneider S2 cells (80% confluent) were cotransfected in 24-well plates with 150 ng of the dicistronic plasmids and 50 ng of the pActGal4 plasmid by using the Effectene transfection reagent (Qiagen). .. After 48 h of transfection, the fluorescence signal was acquired using a Zeiss LSM 310 confocal microscope and the appropriate filters set.

Software:

Article Title: Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice
Article Snippet: The fluorescence signal of the cells was recorded using an Axiovert 200M fluorescence microscope (Carl Zeiss MicroImaging, Inc., Thornwood, NY) equipped with a GFP filter set (Exciter, HQ 475/20 nm; Emitter, HQ 540/30 nm). .. Images were taken using a thermoelectrically cooled charged-coupled device (CCD) (Micromax, model RTE/CCD-576, Princeton Instruments Inc., Trenton, NJ) and analyzed using MetaMorph Software version 6.2r4 (Molecular Devices Corporation, Downingtown, PA).

Article Title: Diminished Superoxide Generation Is Associated With Respiratory Chain Dysfunction and Changes in the Mitochondrial Proteome of Sensory Neurons From Diabetic Rats
Article Snippet: Cultured DRG neurons were loaded with 3.0 nmol/l tetramethyl rhodamine methyl ester (TMRM; Molecular Probes, Eugene, OR) for 1 h and the fluorescence signal in the axons detected with a Carl Zeiss LSM510 confocal inverted microscope (X100 objective; excitation at 540 nm and emission > 560 nm). .. All axons in each field were assessed as the average of fluorescence pixel intensity per axon length using the Carl Zeiss software package ( ).

Article Title: Recycling of IRAP from the plasma membrane back to the insulin-responsive compartment requires the Q-SNARE syntaxin 6 but not the GGA clathrin adaptors
Article Snippet: .. Quantification of the fluorescence signal at the cell surface versus the total-cell fluorescence was performed using the Zeiss LSM software package. ..

Evaporation:

Article Title: Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation
Article Snippet: Solvent evaporation from the channel ends after capillary flow maintained some additional flow. .. The experimental setup used to record fluorescence signal included an upright Axio Scope.A1 microscope (Zeiss, Thornwood, NY) fitted with a 625 mW blue LED light source (470 nm center wavelength, MBLED, Thorlabs, Newton, NJ).

Concentration Assay:

Article Title: The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila
Article Snippet: 3kD dextran coupled to rhodamine (Thermo-Fisher) were dissolved at a 10 mg/ml concentration in Sörensen injection buffer. .. Starting immediately after injection, behaviour of the fluorescence signal was monitored for about one hour using a Zeiss Axiophot microscope.

Article Title: Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation
Article Snippet: Each device design was used to test four different concentrations (0.05, 0.1, 0.5, and 1.0 mg/mL) of each protein, and each concentration was run on three different devices from the same wafer to assess reproducibility. .. The experimental setup used to record fluorescence signal included an upright Axio Scope.A1 microscope (Zeiss, Thornwood, NY) fitted with a 625 mW blue LED light source (470 nm center wavelength, MBLED, Thorlabs, Newton, NJ).

Article Title: Fine Particulate Matter Leads to Unfolded Protein Response and Shortened Lifespan by Inducing Oxidative Stress in C. elegans
Article Snippet: ROS Induction Assessment For ROS induction assessment, worms were washed into the centrifugal tube and CM-H2 DCFDA (C6827, Invitrogen/Molecular Probes) was added to a final concentration of 1 μ M followed by incubating at 20°C for 5 hours in the dark. .. The fluorescence signal was observed under a fluorescence microscope (Zeiss, Axio Observer Z1, Germany) ( excitation wavelength: 480 nm; emission wavelength: 510 nm), and the intensities of the relative fluorescent units (RFU) in the intestine were measured and quantified by the ImageJ program (NIH, Bethesda, MD).

Article Title: Diminished Superoxide Generation Is Associated With Respiratory Chain Dysfunction and Changes in the Mitochondrial Proteome of Sensory Neurons From Diabetic Rats
Article Snippet: Cultured DRG neurons were loaded with 3.0 nmol/l tetramethyl rhodamine methyl ester (TMRM; Molecular Probes, Eugene, OR) for 1 h and the fluorescence signal in the axons detected with a Carl Zeiss LSM510 confocal inverted microscope (X100 objective; excitation at 540 nm and emission > 560 nm). .. Antimycin A and oligomycin (Sigma) were injected into the culture media to a final concentration of 10 μmol/l and 1 μmol/l, respectively, at 1 min after baseline fluorescence measurements.

Fractionation:

Article Title: Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis [OPEN]
Article Snippet: The fluorescence signal of GFP and autofluorescence of chlorophylls were analyzed under a Zeiss LSM 510 Meta confocal microscope with an appropriate filter set. .. Fractionation of chloroplastic compartments was performed as described ( ).

Staining:

Article Title: Embryology of two mycoheterotrophic orchid species, Gastrodia elata and Gastrodia nantoensis: ovule and embryo development
Article Snippet: The sections were stained with 1 μg ml-1 of Nile red (Sigma Chemical Co., St. Louis, Mo.) for 5 min, briefly washed in distilled water for 1 min, and mounted in a solution containing 0.1 % n-propyl gallate (Sigma Chemical Co.), an antifading compound. .. The fluorescence signal was examined using an epifluorescence microscope (Axioskop 2, Carl Zeiss AG) equipped with the Zeiss filter set 15 (546/12 nm excitation filter and 590 emission barrier filter).

Article Title: Luminal Alkalinization Attenuates Proteinuria-Induced Oxidative Damage in Proximal Tubular Cells
Article Snippet: Cells were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1:1000) for nuclear staining. .. The fluorescence signal was observed with a LSM510 confocal imaging system (Carl Zeiss).

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    Carl Zeiss 4 hne fluorescent signal
    H 2 water reduced <t>4-HNE,</t> but not DHE fluorescence in SN after MPTP administration. (A) Fluorescent images of 4-HNE, DHE, and TH in SN (n = 3∼4). Mice treated with non-H 2 water or H 2 /Mg water with injection of saline or MPTP. All samples were acquired 24 h after administration of saline or MPTP. Scale: 50 µm. (B, C) Quantification of 4-HNE (B) and DHE (C) intensity in TH-positive cells in SN. One-way ANOVA; # P
    4 Hne Fluorescent Signal, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4 hne fluorescent signal/product/Carl Zeiss
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    4 hne fluorescent signal - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    94
    Carl Zeiss fluorescence in situ hybridization signals
    <t>Fluorescence</t> <t>in</t> <t>situ</t> <t>hybridization.</t> a, The distribution on a normal X chromosome of the STS locus at the distal tip of the short arm in red, the X centromere (DXZ1) in green, and the proximal long arm signal of XIST in red. This cell has a single X chromosome. b, A cell with an X,r(X) complement showing the 3 expected <t>signals</t> on the normal X chromosome, and an X centromere signal and the proximal XIST locus on the ring (arrow). c, A cell with an X,der(X)t(X;X) complement showing the 3 expected signals on the normal X, and only the X centromere signal and the proximal XIST locus on the larger and more metacentric der(X)t(X;X) chromosome (arrow). The STS locus on the short arm of the der(X)t(X;X) chromosome has been lost in the rearrangement.
    Fluorescence In Situ Hybridization Signals, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence in situ hybridization signals/product/Carl Zeiss
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescence in situ hybridization signals - by Bioz Stars, 2020-04
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    94
    Carl Zeiss ih gfp fluorescence signals
    Rice NADP-ME2 Is Involved in the Accumulation of ROS and Ferric Ions (Fe 3+ ) in HR Cell Death during Avirulent M. oryzae Infection. (A) and (B) CM-H 2 <t>DCFDA</t> (GF) (A) and Prussian blue (blue color) (B) staining shows the accumulation of H 2 O 2 and ferric ion (Fe 3+ ) around IH in wild-type rice (cv HY) during avirulent M. oryzae INA168 infection. By contrast, the focal accumulation of H 2 O 2 and ferric ion (Fe 3+ ) was not detected in ΔOs-nadp-me2-3 mutant rice. (C) Avirulent M. oryzae <t>INA168:GFP</t> induces HR cell death in wild-type rice but successfully colonizes ΔOs-nadp-me2-3 mutant rice. Images in (A) and (C) were taken by a fluorescence microscope (Zeiss equipped with Axioplan 2) using a bright field (BF) as well as a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters. Images in (B) were taken by a fluorescence microscope using a bright field. The images shown are representative of the different leaf sheath samples that were analyzed in three independent experiments. Bars = 20 μm.
    Ih Gfp Fluorescence Signals, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Carl Zeiss fluorescent tunel signal
    Apoptosis in ciliates. <t>TUNEL</t> assay in E. aediculatus treated with 2 μg/mL AN2 or vehicle (CTRL) for 2 h. <t>DAPI</t> (blue) was used for nuclei detection. The images are representative of 10 independent experiments. Scale bar = 30 µm.
    Fluorescent Tunel Signal, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    H 2 water reduced 4-HNE, but not DHE fluorescence in SN after MPTP administration. (A) Fluorescent images of 4-HNE, DHE, and TH in SN (n = 3∼4). Mice treated with non-H 2 water or H 2 /Mg water with injection of saline or MPTP. All samples were acquired 24 h after administration of saline or MPTP. Scale: 50 µm. (B, C) Quantification of 4-HNE (B) and DHE (C) intensity in TH-positive cells in SN. One-way ANOVA; # P

    Journal: PLoS ONE

    Article Title: Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Mouse Model of Parkinson's Disease

    doi: 10.1371/journal.pone.0007247

    Figure Lengend Snippet: H 2 water reduced 4-HNE, but not DHE fluorescence in SN after MPTP administration. (A) Fluorescent images of 4-HNE, DHE, and TH in SN (n = 3∼4). Mice treated with non-H 2 water or H 2 /Mg water with injection of saline or MPTP. All samples were acquired 24 h after administration of saline or MPTP. Scale: 50 µm. (B, C) Quantification of 4-HNE (B) and DHE (C) intensity in TH-positive cells in SN. One-way ANOVA; # P

    Article Snippet: Quantitative analysis of DHE and 4-HNE fluorescent signal All samples were analyzed with a confocal laser scanning microscope (LSM510META, Carl Zeiss, Germany).

    Techniques: Fluorescence, Mouse Assay, Injection

    Fluorescence in situ hybridization. a, The distribution on a normal X chromosome of the STS locus at the distal tip of the short arm in red, the X centromere (DXZ1) in green, and the proximal long arm signal of XIST in red. This cell has a single X chromosome. b, A cell with an X,r(X) complement showing the 3 expected signals on the normal X chromosome, and an X centromere signal and the proximal XIST locus on the ring (arrow). c, A cell with an X,der(X)t(X;X) complement showing the 3 expected signals on the normal X, and only the X centromere signal and the proximal XIST locus on the larger and more metacentric der(X)t(X;X) chromosome (arrow). The STS locus on the short arm of the der(X)t(X;X) chromosome has been lost in the rearrangement.

    Journal: Archives of pathology & laboratory medicine

    Article Title: Extensive Analysis of Mosaicism in a Case of Turner Syndrome

    doi: 10.1043/0003-9985(1999)123 < 0381:EAOMIA > 2.0.CO;2

    Figure Lengend Snippet: Fluorescence in situ hybridization. a, The distribution on a normal X chromosome of the STS locus at the distal tip of the short arm in red, the X centromere (DXZ1) in green, and the proximal long arm signal of XIST in red. This cell has a single X chromosome. b, A cell with an X,r(X) complement showing the 3 expected signals on the normal X chromosome, and an X centromere signal and the proximal XIST locus on the ring (arrow). c, A cell with an X,der(X)t(X;X) complement showing the 3 expected signals on the normal X, and only the X centromere signal and the proximal XIST locus on the larger and more metacentric der(X)t(X;X) chromosome (arrow). The STS locus on the short arm of the der(X)t(X;X) chromosome has been lost in the rearrangement.

    Article Snippet: Fluorescence in situ hybridization signals were captured using a monochromatic CCD camera mounted on a Zeiss epifluorescence microscope with an LUDL filter wheel and a fixed, multi-band-pass beam splitter using MacProbe software (Perceptive Scientific Instruments, Houston, Tex).

    Techniques: Fluorescence, In Situ Hybridization

    Rice NADP-ME2 Is Involved in the Accumulation of ROS and Ferric Ions (Fe 3+ ) in HR Cell Death during Avirulent M. oryzae Infection. (A) and (B) CM-H 2 DCFDA (GF) (A) and Prussian blue (blue color) (B) staining shows the accumulation of H 2 O 2 and ferric ion (Fe 3+ ) around IH in wild-type rice (cv HY) during avirulent M. oryzae INA168 infection. By contrast, the focal accumulation of H 2 O 2 and ferric ion (Fe 3+ ) was not detected in ΔOs-nadp-me2-3 mutant rice. (C) Avirulent M. oryzae INA168:GFP induces HR cell death in wild-type rice but successfully colonizes ΔOs-nadp-me2-3 mutant rice. Images in (A) and (C) were taken by a fluorescence microscope (Zeiss equipped with Axioplan 2) using a bright field (BF) as well as a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters. Images in (B) were taken by a fluorescence microscope using a bright field. The images shown are representative of the different leaf sheath samples that were analyzed in three independent experiments. Bars = 20 μm.

    Journal: The Plant Cell

    Article Title: Iron- and Reactive Oxygen Species-Dependent Ferroptotic Cell Death in Rice-Magnaporthe oryzae Interactions

    doi: 10.1105/tpc.18.00535

    Figure Lengend Snippet: Rice NADP-ME2 Is Involved in the Accumulation of ROS and Ferric Ions (Fe 3+ ) in HR Cell Death during Avirulent M. oryzae Infection. (A) and (B) CM-H 2 DCFDA (GF) (A) and Prussian blue (blue color) (B) staining shows the accumulation of H 2 O 2 and ferric ion (Fe 3+ ) around IH in wild-type rice (cv HY) during avirulent M. oryzae INA168 infection. By contrast, the focal accumulation of H 2 O 2 and ferric ion (Fe 3+ ) was not detected in ΔOs-nadp-me2-3 mutant rice. (C) Avirulent M. oryzae INA168:GFP induces HR cell death in wild-type rice but successfully colonizes ΔOs-nadp-me2-3 mutant rice. Images in (A) and (C) were taken by a fluorescence microscope (Zeiss equipped with Axioplan 2) using a bright field (BF) as well as a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters. Images in (B) were taken by a fluorescence microscope using a bright field. The images shown are representative of the different leaf sheath samples that were analyzed in three independent experiments. Bars = 20 μm.

    Article Snippet: Both CM-H2 DCFDA-specific and IH:GFP fluorescence signals were visualized with the Zeiss fluorescence microscope using a combination of excitation (wavelengths, 450‒490 nm) and emission (515‒565 nm) GF filters.

    Techniques: Infection, Staining, Mutagenesis, Fluorescence, Microscopy

    Apoptosis in ciliates. TUNEL assay in E. aediculatus treated with 2 μg/mL AN2 or vehicle (CTRL) for 2 h. DAPI (blue) was used for nuclei detection. The images are representative of 10 independent experiments. Scale bar = 30 µm.

    Journal: Toxins

    Article Title: Bioactivity and Structural Properties of Novel Synthetic Analogues of the Protozoan Toxin Climacostol

    doi: 10.3390/toxins11010042

    Figure Lengend Snippet: Apoptosis in ciliates. TUNEL assay in E. aediculatus treated with 2 μg/mL AN2 or vehicle (CTRL) for 2 h. DAPI (blue) was used for nuclei detection. The images are representative of 10 independent experiments. Scale bar = 30 µm.

    Article Snippet: DAPI staining, fluorescent TUNEL signal, and the corresponding bright field images were acquired using a Zeiss microscope (Axioskop 2 plus, Carl Zeiss, Oberkochen, Germany) equipped with the Axiocam MRC photocamera and the Axiovision software.

    Techniques: TUNEL Assay