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Signal Recovery fluorescence signal recovery
Fluorescence Signal Recovery, supplied by Signal Recovery, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amplification:

Article Title: mRNA-Initiated, Three-Dimensional DNA Amplifier Able to Function inside Living Cells
Article Snippet: Then strand P7 on the FT promptly binds to the exposed domain 2* on strand P5 and immediately triggers a new cascade strand-displacement reaction, leading to an intense fluorescence signal recovery owing to the effective physical separation of FAM from Dabcyl. .. Unlike prevailing hairpin-based DNA amplification systems, these studies show that the newly developed hairpin-free signal amplification strategy is driven forward by entropy increase rather than by the free energy released by the formation of new base pairs.

Synthesized:

Article Title: A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery
Article Snippet: SQ-ds-siRNA was also synthesized by assembling Cy3-tagged siRNA and BHQ2-tagged R1 and R2, and used as control. .. The mixture was incubated at 37 °C for 12 h, fluorescence signal recovery was observed with the disassembly of SQ-siRNA-ONV nanotube and release of siRNA.

Blocking Assay:

Article Title: The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis [C] [C] [W]
Article Snippet: To examine whether lateral diffusion of PIN2-EGFP on the plasma membrane could contribute to the observed differences in the fluorescence recovery rates of wild-type and rsw4 root cortex cells, we bleached a small area of a PIN2-EGFP–positive rootward plasma membrane (see online, samples Control and ) and found no difference in fluorescence signal recovery between the wild type and rsw4 . .. Next, we repeated the experiment in cells treated with trafficking inhibitors sodium azide and deoxy- d -Glc, which block the energy-dependent trafficking of molecules ( ).

End-sequence Profiling:

Article Title: The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis [C] [C] [W]
Article Snippet: This suggests that ESP deficiency does not cause general membrane trafficking defects, but specifically affects polarized protein delivery. .. To examine whether lateral diffusion of PIN2-EGFP on the plasma membrane could contribute to the observed differences in the fluorescence recovery rates of wild-type and rsw4 root cortex cells, we bleached a small area of a PIN2-EGFP–positive rootward plasma membrane (see online, samples Control and ) and found no difference in fluorescence signal recovery between the wild type and rsw4 .

Incubation:

Article Title: A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery
Article Snippet: .. The mixture was incubated at 37 °C for 12 h, fluorescence signal recovery was observed with the disassembly of SQ-siRNA-ONV nanotube and release of siRNA. .. The fluorescence signal recovery was measured every hour with 510 nm excitation and 560 nm emission.

Article Title: Dual-stimuli responsive and reversibly activatable theranostic nanoprobe for precision tumor-targeting and fluorescence-guided photothermal therapy
Article Snippet: Incubation of Pep-Acy/Glu@AuNRs and various types of MMPs with simultaneous adjusting pH to 6.0 (simulated tumour acidic microenvironment) activated the near-infrared fluorescence. .. The fluorescence signal recovery of Pep-Acy/Glu@AuNRs was closely correlated with the concentration of MMP-13 in an acidic microenvironment ( ).

Diffusion-based Assay:

Article Title: FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation
Article Snippet: .. To test this interpretation, we performed fluorescence recovery after photobleaching (FRAP) on ER-trapped VSVGts045 -GFP using the rate of fluorescence signal recovery as an indication of lateral diffusion and ER exit of the VSVG cargo (see ). ..

Article Title: The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis [C] [C] [W]
Article Snippet: .. To examine whether lateral diffusion of PIN2-EGFP on the plasma membrane could contribute to the observed differences in the fluorescence recovery rates of wild-type and rsw4 root cortex cells, we bleached a small area of a PIN2-EGFP–positive rootward plasma membrane (see online, samples Control and ) and found no difference in fluorescence signal recovery between the wild type and rsw4 . .. Next, we repeated the experiment in cells treated with trafficking inhibitors sodium azide and deoxy- d -Glc, which block the energy-dependent trafficking of molecules ( ).

Article Title: Mechanisms of kinetic stabilization by the drugs paclitaxel and vinblastine
Article Snippet: .. Because fluorescence signal recovery from microtubule polymer requires turnover of the dynamic plus end (i.e., disassembly of the bleached region and reincorporation of fluorescent subunits), polymer signal recovery is much slower than recovery of the free tubulin signal by diffusion. .. Therefore, within the analyzed time period, fluorescence signal recovery is almost exclusively due to free tubulin rather than microtubule polymer.

Expressing:

Article Title: Bacterial actins and their diversity
Article Snippet: FRAP assays with ΔMAI cells expressing MamK-GFP from a plasmid show no fluorescence signal recovery, implying that MamK filaments are no longer dynamic . .. In a strain lacking both these proteins, FRAP assays with MamK-GFP again do not show fluorescence signal recovery .

Western Blot:

Article Title: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells
Article Snippet: Importantly, Western blotting analysis showed no increase in the total amount of actin during this period (Figure C), which is indicative of actin redistribution rather than additional de novo synthesis of G-actin. .. The speed of fluorescence signal recovery was measured at several time points after addition of mAbs (Figure , A and B, and Supplemental Videos 2–4).

Hybridization:

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. ) .. Figure reproduced with permission from [ ) Figure reproduced with permission from [ ) Figure reproduced with permission from [ ) Figure reproduced with permission from [ As an alternative direction for developing fluorescent based biosensors, Bracamonte and his group have developed highly fluorescent nanoparticles by utilizing GNPs as the core, silica as the shell (as a spacer), and rhodamine b (RhB) as a fluorophore for bacteria imaging and sensing ( C) [ ].

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. .. As an alternative direction for developing fluorescent based biosensors, Bracamonte and his group have developed highly fluorescent nanoparticles by utilizing GNPs as the core, silica as the shell (as a spacer), and rhodamine b (RhB) as a fluorophore for bacteria imaging and sensing ( C) [ ].

Transfection:

Article Title: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells
Article Snippet: To allow us to assess the kinetics of actin relocalization, we subsequently performed time-lapse microscopy of Raji cells transfected to express a GFP-actin fusion protein. .. The speed of fluorescence signal recovery was measured at several time points after addition of mAbs (Figure , A and B, and Supplemental Videos 2–4).

Gas Chromatography:

Article Title: The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis [C] [C] [W]
Article Snippet: To examine whether lateral diffusion of PIN2-EGFP on the plasma membrane could contribute to the observed differences in the fluorescence recovery rates of wild-type and rsw4 root cortex cells, we bleached a small area of a PIN2-EGFP–positive rootward plasma membrane (see online, samples Control and ) and found no difference in fluorescence signal recovery between the wild type and rsw4 . .. Next, we repeated the experiment in cells treated with trafficking inhibitors sodium azide and deoxy- d -Glc, which block the energy-dependent trafficking of molecules ( ).

Concentration Assay:

Article Title: A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery
Article Snippet: Then, 50 nM of SQ-siRNA-ONV (100 nM fluorophore concentration) and 100 nM of SQ-ds-siRNA were separately diluted with FBS to yield a final concentration of 50 nM fluorophore in 10% FBS 1 × TAMg. .. The mixture was incubated at 37 °C for 12 h, fluorescence signal recovery was observed with the disassembly of SQ-siRNA-ONV nanotube and release of siRNA.

Article Title: Dual-stimuli responsive and reversibly activatable theranostic nanoprobe for precision tumor-targeting and fluorescence-guided photothermal therapy
Article Snippet: .. The fluorescence signal recovery of Pep-Acy/Glu@AuNRs was closely correlated with the concentration of MMP-13 in an acidic microenvironment ( ). .. In contrast, no fluorescence of Pep-Acy/Glu@AuNRs was lightened up with MMP-13 in weak basic medium (pH 7.4, normal biological fluid pH) although the peptide was enzymolyzed in the presence of MMP-13 because the detached Acy was in its basic non-fluorescent form.

Article Title: Mechanisms of kinetic stabilization by the drugs paclitaxel and vinblastine
Article Snippet: Paragraph title: MTAs moderately influence free tubulin concentration in individual cells in vivo ... Because fluorescence signal recovery from microtubule polymer requires turnover of the dynamic plus end (i.e., disassembly of the bleached region and reincorporation of fluorescent subunits), polymer signal recovery is much slower than recovery of the free tubulin signal by diffusion.

Article Title: Development of Structure-Switching Aptamers for Kanamycin Detection Based on Fluorescence Resonance Energy Transfer
Article Snippet: .. Under optimal conditions, there was a good linear relationship between kanamycin concentration and the fluorescence signal recovery. ..

Article Title: Structural Basis for the Aminoacid Composition of Proteins from Halophilic ArcheaSurviving Salt: How Do Extremophiles Do It?
Article Snippet: Thermal denaturation curves monitored by fluorescence spectroscopy were collected at a concentration range of 1–2 µM, with measuring conditions equivalent to the CD experiments except for the use of a 2 nm excitation bandwidth centered at 280 nm. .. In all cases, the CD and fluorescence signal recovery after the thermal melt was monitored and is reported in the supplementary materials ( , , ).

Generated:

Article Title: Image-guided surgery using near-infrared Turn-ON fluorescent nanoprobes for precise detection of tumor margins
Article Snippet: PCQ was designed to be generated from the former PC by the addition of quencher molecules, thus introducing FRET quenching interactions into this system (system 3). .. We sought to compare the two systems' fluorescence signal recovery upon challenging with CTSB in vitro and cathepsins in vivo to determine their potential application as guiding agents for real-time image-guided surgery.

Inhibition:

Article Title: FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation
Article Snippet: Paragraph title: FFAT rescues VAPA-mediated inhibition of lateral diffusion of membrane proteins in the ER ... To test this interpretation, we performed fluorescence recovery after photobleaching (FRAP) on ER-trapped VSVGts045 -GFP using the rate of fluorescence signal recovery as an indication of lateral diffusion and ER exit of the VSVG cargo (see ).

Imaging:

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. ) .. Figure reproduced with permission from [ ) Figure reproduced with permission from [ ) Figure reproduced with permission from [ ) Figure reproduced with permission from [ As an alternative direction for developing fluorescent based biosensors, Bracamonte and his group have developed highly fluorescent nanoparticles by utilizing GNPs as the core, silica as the shell (as a spacer), and rhodamine b (RhB) as a fluorophore for bacteria imaging and sensing ( C) [ ].

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. .. As an alternative direction for developing fluorescent based biosensors, Bracamonte and his group have developed highly fluorescent nanoparticles by utilizing GNPs as the core, silica as the shell (as a spacer), and rhodamine b (RhB) as a fluorophore for bacteria imaging and sensing ( C) [ ].

Protein Concentration:

Article Title: Structural Basis for the Aminoacid Composition of Proteins from Halophilic ArcheaSurviving Salt: How Do Extremophiles Do It?
Article Snippet: In all cases, the CD and fluorescence signal recovery after the thermal melt was monitored and is reported in the supplementary materials ( , , ). .. Guanidinium chloride or urea denaturation experiments were followed by CD and used an initial volume of 1.7–2 mL at a protein concentration of 4 µM in 20 mM phosphate buffer at pH 6.0.

Sequencing:

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: Initially, fluorescence associated with the fluorophore was quenched due to the FRET between the fluorophores and the GNPs; however, when the PSA recognized and cleaved the specific sequence of the peptides conjugated to the GNP, a recovery of fluorescence signal was observed due to the splitting of the FITCs from the GNP. .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. )

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: Initially, fluorescence associated with the fluorophore was quenched due to the FRET between the fluorophores and the GNPs; however, when the PSA recognized and cleaved the specific sequence of the peptides conjugated to the GNP, a recovery of fluorescence signal was observed due to the splitting of the FITCs from the GNP. .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs.

Article Title: mRNA-Initiated, Three-Dimensional DNA Amplifier Able to Function inside Living Cells
Article Snippet: However, P7 had a different extended sequence. .. Then strand P7 on the FT promptly binds to the exposed domain 2* on strand P5 and immediately triggers a new cascade strand-displacement reaction, leading to an intense fluorescence signal recovery owing to the effective physical separation of FAM from Dabcyl.

In Vivo:

Article Title: Image-guided surgery using near-infrared Turn-ON fluorescent nanoprobes for precise detection of tumor margins
Article Snippet: .. We sought to compare the two systems' fluorescence signal recovery upon challenging with CTSB in vitro and cathepsins in vivo to determine their potential application as guiding agents for real-time image-guided surgery. ..

Article Title: Bacterial actins and their diversity
Article Snippet: Paragraph title: The dynamic behavior of MamK filaments in vivo ... In a strain lacking both these proteins, FRAP assays with MamK-GFP again do not show fluorescence signal recovery .

Article Title: Mechanisms of kinetic stabilization by the drugs paclitaxel and vinblastine
Article Snippet: Paragraph title: MTAs moderately influence free tubulin concentration in individual cells in vivo ... Because fluorescence signal recovery from microtubule polymer requires turnover of the dynamic plus end (i.e., disassembly of the bleached region and reincorporation of fluorescent subunits), polymer signal recovery is much slower than recovery of the free tubulin signal by diffusion.

Fluorescence:

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. ) .. Figure reproduced with permission from [ ) Figure reproduced with permission from [ ) Figure reproduced with permission from [ ) Figure reproduced with permission from [ As an alternative direction for developing fluorescent based biosensors, Bracamonte and his group have developed highly fluorescent nanoparticles by utilizing GNPs as the core, silica as the shell (as a spacer), and rhodamine b (RhB) as a fluorophore for bacteria imaging and sensing ( C) [ ].

Article Title: Image-guided surgery using near-infrared Turn-ON fluorescent nanoprobes for precise detection of tumor margins
Article Snippet: .. We sought to compare the two systems' fluorescence signal recovery upon challenging with CTSB in vitro and cathepsins in vivo to determine their potential application as guiding agents for real-time image-guided surgery. ..

Article Title: FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation
Article Snippet: .. To test this interpretation, we performed fluorescence recovery after photobleaching (FRAP) on ER-trapped VSVGts045 -GFP using the rate of fluorescence signal recovery as an indication of lateral diffusion and ER exit of the VSVG cargo (see ). ..

Article Title: A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery
Article Snippet: .. The mixture was incubated at 37 °C for 12 h, fluorescence signal recovery was observed with the disassembly of SQ-siRNA-ONV nanotube and release of siRNA. .. The fluorescence signal recovery was measured every hour with 510 nm excitation and 560 nm emission.

Article Title: The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis [C] [C] [W]
Article Snippet: .. To examine whether lateral diffusion of PIN2-EGFP on the plasma membrane could contribute to the observed differences in the fluorescence recovery rates of wild-type and rsw4 root cortex cells, we bleached a small area of a PIN2-EGFP–positive rootward plasma membrane (see online, samples Control and ) and found no difference in fluorescence signal recovery between the wild type and rsw4 . .. Next, we repeated the experiment in cells treated with trafficking inhibitors sodium azide and deoxy- d -Glc, which block the energy-dependent trafficking of molecules ( ).

Article Title: Bacterial actins and their diversity
Article Snippet: .. In a strain lacking both these proteins, FRAP assays with MamK-GFP again do not show fluorescence signal recovery . ..

Article Title: Dual-stimuli responsive and reversibly activatable theranostic nanoprobe for precision tumor-targeting and fluorescence-guided photothermal therapy
Article Snippet: .. The fluorescence signal recovery of Pep-Acy/Glu@AuNRs was closely correlated with the concentration of MMP-13 in an acidic microenvironment ( ). .. In contrast, no fluorescence of Pep-Acy/Glu@AuNRs was lightened up with MMP-13 in weak basic medium (pH 7.4, normal biological fluid pH) although the peptide was enzymolyzed in the presence of MMP-13 because the detached Acy was in its basic non-fluorescent form.

Article Title: Mechanisms of kinetic stabilization by the drugs paclitaxel and vinblastine
Article Snippet: .. Because fluorescence signal recovery from microtubule polymer requires turnover of the dynamic plus end (i.e., disassembly of the bleached region and reincorporation of fluorescent subunits), polymer signal recovery is much slower than recovery of the free tubulin signal by diffusion. .. Therefore, within the analyzed time period, fluorescence signal recovery is almost exclusively due to free tubulin rather than microtubule polymer.

Article Title: Real-time observation of backtracking by bacterial RNA polymerase
Article Snippet: .. We interpret this fluorescence signal recovery as a esult of a slow accumulation of backtracked RNAP. .. The time-dependence of fluorescence observed upon addition of ATP, UTP and GTP is thus a net effect of escape and backtracking.

Article Title: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells
Article Snippet: .. The speed of fluorescence signal recovery was measured at several time points after addition of mAbs (Figure , A and B, and Supplemental Videos 2–4). .. These data indicate that the recovery of actin fluorescence is much faster in cells treated with tositumomab or L243 mAbs than in control cells, in which no recovery could be observed within 100 seconds.

Article Title: Development of Structure-Switching Aptamers for Kanamycin Detection Based on Fluorescence Resonance Energy Transfer
Article Snippet: .. Under optimal conditions, there was a good linear relationship between kanamycin concentration and the fluorescence signal recovery. ..

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. .. As an alternative direction for developing fluorescent based biosensors, Bracamonte and his group have developed highly fluorescent nanoparticles by utilizing GNPs as the core, silica as the shell (as a spacer), and rhodamine b (RhB) as a fluorophore for bacteria imaging and sensing ( C) [ ].

Article Title: Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance
Article Snippet: .. FRAP corresponds to the time-resolved analysis of fluorescence signal recovery in an intentionally bleached area . .. Free diffusing fluorophores will stochastically diffuse into the bleached area and induce a time-dependent signal recovery.

Article Title: Structural Basis for the Aminoacid Composition of Proteins from Halophilic ArcheaSurviving Salt: How Do Extremophiles Do It?
Article Snippet: .. In all cases, the CD and fluorescence signal recovery after the thermal melt was monitored and is reported in the supplementary materials ( , , ). ..

Article Title: mRNA-Initiated, Three-Dimensional DNA Amplifier Able to Function inside Living Cells
Article Snippet: .. Then strand P7 on the FT promptly binds to the exposed domain 2* on strand P5 and immediately triggers a new cascade strand-displacement reaction, leading to an intense fluorescence signal recovery owing to the effective physical separation of FAM from Dabcyl. ..

Flow Cytometry:

Article Title: Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance
Article Snippet: Rho-PE signal fully covered the region corresponding to the sensor chip flow cells and localized in the YZ plane as a continuous layer with constant thickness (~20 μm) ( ). .. FRAP corresponds to the time-resolved analysis of fluorescence signal recovery in an intentionally bleached area .

Construct:

Article Title: Real-time observation of backtracking by bacterial RNA polymerase
Article Snippet: Paragraph title: Design of fluorescent promoter construct for real-time monitoring of promoter escape ... We interpret this fluorescence signal recovery as a esult of a slow accumulation of backtracked RNAP.

Spectroscopy:

Article Title: Structural Basis for the Aminoacid Composition of Proteins from Halophilic ArcheaSurviving Salt: How Do Extremophiles Do It?
Article Snippet: Thermal denaturation curves monitored by fluorescence spectroscopy were collected at a concentration range of 1–2 µM, with measuring conditions equivalent to the CD experiments except for the use of a 2 nm excitation bandwidth centered at 280 nm. .. In all cases, the CD and fluorescence signal recovery after the thermal melt was monitored and is reported in the supplementary materials ( , , ).

Chromatin Immunoprecipitation:

Article Title: Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance
Article Snippet: Paragraph title: Validating L1 sensor chip coverage ... FRAP corresponds to the time-resolved analysis of fluorescence signal recovery in an intentionally bleached area .

Plasmid Preparation:

Article Title: Bacterial actins and their diversity
Article Snippet: FRAP assays with ΔMAI cells expressing MamK-GFP from a plasmid show no fluorescence signal recovery, implying that MamK filaments are no longer dynamic . .. In a strain lacking both these proteins, FRAP assays with MamK-GFP again do not show fluorescence signal recovery .

Functional Assay:

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: A small peptide sequence with a fluorophore (fluorescein isothiocyanate, FITC) on one end was conjugated to the GNP through the functional amine group on the other end. .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs. )

Article Title: Application of Gold Nanoparticle to Plasmonic Biosensors
Article Snippet: A small peptide sequence with a fluorophore (fluorescein isothiocyanate, FITC) on one end was conjugated to the GNP through the functional amine group on the other end. .. The presence of target miRNA led to the hybridization of DNA–RNA strands, and, following enzyme hydrolysis, fluorescence signal recovery was observed by splitting fluorophores from GNPs.

In Vitro:

Article Title: Image-guided surgery using near-infrared Turn-ON fluorescent nanoprobes for precise detection of tumor margins
Article Snippet: .. We sought to compare the two systems' fluorescence signal recovery upon challenging with CTSB in vitro and cathepsins in vivo to determine their potential application as guiding agents for real-time image-guided surgery. ..

Article Title: Dual-stimuli responsive and reversibly activatable theranostic nanoprobe for precision tumor-targeting and fluorescence-guided photothermal therapy
Article Snippet: MMP-13 was therefore chosen as a typical MMP for further in vitro test due to its high sensitivity. .. The fluorescence signal recovery of Pep-Acy/Glu@AuNRs was closely correlated with the concentration of MMP-13 in an acidic microenvironment ( ).

Time-lapse Microscopy:

Article Title: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells
Article Snippet: To allow us to assess the kinetics of actin relocalization, we subsequently performed time-lapse microscopy of Raji cells transfected to express a GFP-actin fusion protein. .. The speed of fluorescence signal recovery was measured at several time points after addition of mAbs (Figure , A and B, and Supplemental Videos 2–4).

Activation Assay:

Article Title: Image-guided surgery using near-infrared Turn-ON fluorescent nanoprobes for precise detection of tumor margins
Article Snippet: The first activation is by the GFLG linker cleavage and the second is by backbone degradation ( Figure A ). .. We sought to compare the two systems' fluorescence signal recovery upon challenging with CTSB in vitro and cathepsins in vivo to determine their potential application as guiding agents for real-time image-guided surgery.

Article Title: Dual-stimuli responsive and reversibly activatable theranostic nanoprobe for precision tumor-targeting and fluorescence-guided photothermal therapy
Article Snippet: Paragraph title: Dual-stimuli responsive and reversible activation ... The fluorescence signal recovery of Pep-Acy/Glu@AuNRs was closely correlated with the concentration of MMP-13 in an acidic microenvironment ( ).

Staining:

Article Title: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells
Article Snippet: Furthermore, careful analysis of the actin staining pattern showed a decrease in the fluorescence signal area (Figure D), suggesting that compartmentalization rather than de novo synthesis of cellular F-actin is responsible for the increased actin signal at the cell-cell contact area. .. The speed of fluorescence signal recovery was measured at several time points after addition of mAbs (Figure , A and B, and Supplemental Videos 2–4).

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  • 80
    Signal Recovery mmp 2 mediated mmp p12 fluorescent signal recovery
    Co-localization of <t>MMP-2</t> expression and cleaved <t>MMP-P12</t> biodistribution in normal (PBS), fibrotic (BLM) and fibrosis recovered (BLM+SCGB3A2) lung sections. Blue (DAPI) stains nuclei, red color (Cy3) shows the expression of MMP-2, and green color (Cy5.5) shows the biodistribution of MMP-P12 in lung section. The Cy3 and Cy5.5 merged images are seen in yellow (Merge), demonstrating that a majority of red and green signals overlap. Original magnification: ×400. Scale bar: 10 μm.
    Mmp 2 Mediated Mmp P12 Fluorescent Signal Recovery, supplied by Signal Recovery, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp 2 mediated mmp p12 fluorescent signal recovery/product/Signal Recovery
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    81
    Signal Recovery fluorescence intensity drop signaling rnap escape
    The effect of nonhydrolizable analogue of CTP (CpCpp) on <t>RNAP</t> backtracking on P27 construct. (A) Transcription reactions were initiated by adding <t>ATP,</t> UTP, GTP, heparin and no CpCpp (a), 50 μM CpCpp (b), 150 μM CpCpp (c) or 300 μM CpCpp (d). Curve (e) corresponds to transcription initiated by adding ATP, UTP, GTP, CTP and heparin. (B) Transcription reactions were initiated by adding ATP, UTP, GTP, heparin. CpCpp (150 μM) was added together with NTP’s (a) or after 2 min (b) or 5 min (c) delay after addition of NTP’s. Delayed addition of CpCpp is indicated by the arrows.
    Fluorescence Intensity Drop Signaling Rnap Escape, supplied by Signal Recovery, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Signal Recovery nir fluorescence signal recovery
    (A) <t>MMP-sensitive</t> NS and (B) in vivo <t>NIR</t> fluorescence tomographic images of subcutaneous SCC7 tumor-bearing mice after intravenous injection of the NS (Lee et al., 2009 ), © 2009, American Chemical Society.
    Nir Fluorescence Signal Recovery, supplied by Signal Recovery, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Co-localization of MMP-2 expression and cleaved MMP-P12 biodistribution in normal (PBS), fibrotic (BLM) and fibrosis recovered (BLM+SCGB3A2) lung sections. Blue (DAPI) stains nuclei, red color (Cy3) shows the expression of MMP-2, and green color (Cy5.5) shows the biodistribution of MMP-P12 in lung section. The Cy3 and Cy5.5 merged images are seen in yellow (Merge), demonstrating that a majority of red and green signals overlap. Original magnification: ×400. Scale bar: 10 μm.

    Journal: Molecular pharmaceutics

    Article Title: Non-Invasive Monitoring of Pulmonary Fibrosis by Targeting Matrix Metalloproteinases (MMPs)

    doi: 10.1021/mp300613x

    Figure Lengend Snippet: Co-localization of MMP-2 expression and cleaved MMP-P12 biodistribution in normal (PBS), fibrotic (BLM) and fibrosis recovered (BLM+SCGB3A2) lung sections. Blue (DAPI) stains nuclei, red color (Cy3) shows the expression of MMP-2, and green color (Cy5.5) shows the biodistribution of MMP-P12 in lung section. The Cy3 and Cy5.5 merged images are seen in yellow (Merge), demonstrating that a majority of red and green signals overlap. Original magnification: ×400. Scale bar: 10 μm.

    Article Snippet: In order to confirm that the fluorescent signal indeed came from MMP-2-mediated MMP-P12 fluorescent signal recovery, lung sections were prepared from fibrotic lungs for MMP-2 immunofluorescence ( , red color) and the direct fluorescence signal derived from MMP-P12 cleavage (green color).

    Techniques: Expressing

    In vivo detection of PF at different stages. (A) Chemical structure of MMP-P12. (B) Experimental protocol for inducement and visualization of PF in mouse model. (C) In vivo imaging of lung fibrosis at different stages (7, 14 and 21 days post-BLM administration). Mice were intravenously injected with 100 nmol of MMP-P12 and 2 h later, imaged by Maestro 2.0. Fluorescence intensity became stronger as fibrosis developed. All the images were compared under the same condition. The color bar indicates radiant efficiency (low, 0; high, 0.0905×10 6 ). (D) Quantification of fluorescence intensity in the region of interest (ROI) shown in C (dotted circle). Compared to control group (PBS treated lungs), 3.86±0.36, 7.11±1.21, and 12.99±0.7-fold higher fluorescence signals were obtained at 7, 14, and 21 day post-BLM lungs. n = 5. *, P

    Journal: Molecular pharmaceutics

    Article Title: Non-Invasive Monitoring of Pulmonary Fibrosis by Targeting Matrix Metalloproteinases (MMPs)

    doi: 10.1021/mp300613x

    Figure Lengend Snippet: In vivo detection of PF at different stages. (A) Chemical structure of MMP-P12. (B) Experimental protocol for inducement and visualization of PF in mouse model. (C) In vivo imaging of lung fibrosis at different stages (7, 14 and 21 days post-BLM administration). Mice were intravenously injected with 100 nmol of MMP-P12 and 2 h later, imaged by Maestro 2.0. Fluorescence intensity became stronger as fibrosis developed. All the images were compared under the same condition. The color bar indicates radiant efficiency (low, 0; high, 0.0905×10 6 ). (D) Quantification of fluorescence intensity in the region of interest (ROI) shown in C (dotted circle). Compared to control group (PBS treated lungs), 3.86±0.36, 7.11±1.21, and 12.99±0.7-fold higher fluorescence signals were obtained at 7, 14, and 21 day post-BLM lungs. n = 5. *, P

    Article Snippet: In order to confirm that the fluorescent signal indeed came from MMP-2-mediated MMP-P12 fluorescent signal recovery, lung sections were prepared from fibrotic lungs for MMP-2 immunofluorescence ( , red color) and the direct fluorescence signal derived from MMP-P12 cleavage (green color).

    Techniques: In Vivo, In Vivo Imaging, Mouse Assay, Injection, Fluorescence

    MMP-P12 activations in lung fibrosis model detected ex vivo . (A) Several main organs were removed from the mouse after in vivo imaging, which were put on a black paper for ex vivo analysis. Fibrosis lung at different stages showed different fluorescent signals, while the signals for the other organs remained low except kidney and liver. The color bar indicates radiant efficiency (low, 0; high, 0.147×10 6 ). (B) ROI of each organ (using whole tissue) was quantified at various time points as indicated and was normalized by mouse body weight. Y-axis is the fluorescence signal intensity in arbitrary unit. *, P

    Journal: Molecular pharmaceutics

    Article Title: Non-Invasive Monitoring of Pulmonary Fibrosis by Targeting Matrix Metalloproteinases (MMPs)

    doi: 10.1021/mp300613x

    Figure Lengend Snippet: MMP-P12 activations in lung fibrosis model detected ex vivo . (A) Several main organs were removed from the mouse after in vivo imaging, which were put on a black paper for ex vivo analysis. Fibrosis lung at different stages showed different fluorescent signals, while the signals for the other organs remained low except kidney and liver. The color bar indicates radiant efficiency (low, 0; high, 0.147×10 6 ). (B) ROI of each organ (using whole tissue) was quantified at various time points as indicated and was normalized by mouse body weight. Y-axis is the fluorescence signal intensity in arbitrary unit. *, P

    Article Snippet: In order to confirm that the fluorescent signal indeed came from MMP-2-mediated MMP-P12 fluorescent signal recovery, lung sections were prepared from fibrotic lungs for MMP-2 immunofluorescence ( , red color) and the direct fluorescence signal derived from MMP-P12 cleavage (green color).

    Techniques: Ex Vivo, In Vivo Imaging, Fluorescence

    Response to SCGB3A2 treatment in PF model monitored by MMP-P12. (A) Experimental protocol for inducement, treatment and visualization of PF in mouse model. ( B ) In vivo imaging of lung fibrosis at 21 days in BLM mouse model and those treated with SCGB3A2 by Maestro 2.0 (optical imaging). All images were compared under the same condition. The color bar indicates radiant efficiency (low, 0; high, 0.0726×10 6 ). (C) Quantification of fluorescent signal from in vivo imaging by drawing the whole lung as ROI and was normalized by mouse body weight. Compared to control group, BLM-induced PF with SCGB3A2 treatment mice model showed a decreased fluorescent signal increment (4.82±0.87) compared with that of the PF model without treatment (10.92±2.06). (D) Ex vivo optical imaging of lung tissues from PBS group, BLM induced fibrotic and mSCGB3A2 treated group were compared in the same condition. The color bar indicates radiant efficiency (low, 0; high, 0.101×10 6 ). (E) Quantification of fluorescent signal extracted from lung section and was normalized by mouse body weight. Compared to control group, BLM-induced PF with SCGB3A2 treatment mice model showed a decreased fluorescent signal increment (6.89±1.31) compared with that of PF model without treatment (15.26±1.99). *, P

    Journal: Molecular pharmaceutics

    Article Title: Non-Invasive Monitoring of Pulmonary Fibrosis by Targeting Matrix Metalloproteinases (MMPs)

    doi: 10.1021/mp300613x

    Figure Lengend Snippet: Response to SCGB3A2 treatment in PF model monitored by MMP-P12. (A) Experimental protocol for inducement, treatment and visualization of PF in mouse model. ( B ) In vivo imaging of lung fibrosis at 21 days in BLM mouse model and those treated with SCGB3A2 by Maestro 2.0 (optical imaging). All images were compared under the same condition. The color bar indicates radiant efficiency (low, 0; high, 0.0726×10 6 ). (C) Quantification of fluorescent signal from in vivo imaging by drawing the whole lung as ROI and was normalized by mouse body weight. Compared to control group, BLM-induced PF with SCGB3A2 treatment mice model showed a decreased fluorescent signal increment (4.82±0.87) compared with that of the PF model without treatment (10.92±2.06). (D) Ex vivo optical imaging of lung tissues from PBS group, BLM induced fibrotic and mSCGB3A2 treated group were compared in the same condition. The color bar indicates radiant efficiency (low, 0; high, 0.101×10 6 ). (E) Quantification of fluorescent signal extracted from lung section and was normalized by mouse body weight. Compared to control group, BLM-induced PF with SCGB3A2 treatment mice model showed a decreased fluorescent signal increment (6.89±1.31) compared with that of PF model without treatment (15.26±1.99). *, P

    Article Snippet: In order to confirm that the fluorescent signal indeed came from MMP-2-mediated MMP-P12 fluorescent signal recovery, lung sections were prepared from fibrotic lungs for MMP-2 immunofluorescence ( , red color) and the direct fluorescence signal derived from MMP-P12 cleavage (green color).

    Techniques: In Vivo Imaging, Optical Imaging, Mouse Assay, Ex Vivo

    The effect of nonhydrolizable analogue of CTP (CpCpp) on RNAP backtracking on P27 construct. (A) Transcription reactions were initiated by adding ATP, UTP, GTP, heparin and no CpCpp (a), 50 μM CpCpp (b), 150 μM CpCpp (c) or 300 μM CpCpp (d). Curve (e) corresponds to transcription initiated by adding ATP, UTP, GTP, CTP and heparin. (B) Transcription reactions were initiated by adding ATP, UTP, GTP, heparin. CpCpp (150 μM) was added together with NTP’s (a) or after 2 min (b) or 5 min (c) delay after addition of NTP’s. Delayed addition of CpCpp is indicated by the arrows.

    Journal: Biochemistry

    Article Title: Real-time observation of backtracking by bacterial RNA polymerase

    doi: 10.1021/acs.biochem.5b01184

    Figure Lengend Snippet: The effect of nonhydrolizable analogue of CTP (CpCpp) on RNAP backtracking on P27 construct. (A) Transcription reactions were initiated by adding ATP, UTP, GTP, heparin and no CpCpp (a), 50 μM CpCpp (b), 150 μM CpCpp (c) or 300 μM CpCpp (d). Curve (e) corresponds to transcription initiated by adding ATP, UTP, GTP, CTP and heparin. (B) Transcription reactions were initiated by adding ATP, UTP, GTP, heparin. CpCpp (150 μM) was added together with NTP’s (a) or after 2 min (b) or 5 min (c) delay after addition of NTP’s. Delayed addition of CpCpp is indicated by the arrows.

    Article Snippet: When transcription was initiated with ATP, UTP and GTP in the presence of GreB, only the fluorescence intensity drop signaling RNAP escape was observed and no fluorescence signal recovery phase associated with backtracking was observed ( ).

    Techniques: Construct

    RNAP backtracking during multiple rounds of transcription from P27. At time 0 the P27 was added to a solution of RNAP in a fluorometer cuvette. The star denotes addition of ATP, UTP, GTP and CTP. Heparin was added (as indicated by an arrow) at various delay times after the start of transcription with 4 NTP’s: (A) 15 s, (B) 2 min, (C) 5 min and (D) 20 min. (E) Effect of GreB on the amounts of full-length transcript produced from P27. Transcription was allowed to proceed for 30 min. The data were normalized to the amount of transcript made by P27 under single round of transcription condition (in the presence of heparin).

    Journal: Biochemistry

    Article Title: Real-time observation of backtracking by bacterial RNA polymerase

    doi: 10.1021/acs.biochem.5b01184

    Figure Lengend Snippet: RNAP backtracking during multiple rounds of transcription from P27. At time 0 the P27 was added to a solution of RNAP in a fluorometer cuvette. The star denotes addition of ATP, UTP, GTP and CTP. Heparin was added (as indicated by an arrow) at various delay times after the start of transcription with 4 NTP’s: (A) 15 s, (B) 2 min, (C) 5 min and (D) 20 min. (E) Effect of GreB on the amounts of full-length transcript produced from P27. Transcription was allowed to proceed for 30 min. The data were normalized to the amount of transcript made by P27 under single round of transcription condition (in the presence of heparin).

    Article Snippet: When transcription was initiated with ATP, UTP and GTP in the presence of GreB, only the fluorescence intensity drop signaling RNAP escape was observed and no fluorescence signal recovery phase associated with backtracking was observed ( ).

    Techniques: Produced

    RNAP complexes that accumulate during fluorescence signal recovery phase of the reaction are transcriptionally inactive. The star denotes addition of ATP, UTP, GTP and heparin to the sample. The missing NTP (CTP) was added (as indicated by an arrow) at various delay times after the start of transcription with 3 NTP’s: (A) 15 s, (B) 2 min, (C) 5 min and (D) 20 min.

    Journal: Biochemistry

    Article Title: Real-time observation of backtracking by bacterial RNA polymerase

    doi: 10.1021/acs.biochem.5b01184

    Figure Lengend Snippet: RNAP complexes that accumulate during fluorescence signal recovery phase of the reaction are transcriptionally inactive. The star denotes addition of ATP, UTP, GTP and heparin to the sample. The missing NTP (CTP) was added (as indicated by an arrow) at various delay times after the start of transcription with 3 NTP’s: (A) 15 s, (B) 2 min, (C) 5 min and (D) 20 min.

    Article Snippet: When transcription was initiated with ATP, UTP and GTP in the presence of GreB, only the fluorescence intensity drop signaling RNAP escape was observed and no fluorescence signal recovery phase associated with backtracking was observed ( ).

    Techniques: Fluorescence

    (A) Comparison of calculated relative 9 bp duplex stabilities for P27-31 (cyan), P27-32 (pink), P27-33 (blue), P27-34 (green), and P27-35 (red) constructs with P27 construct (black). (B) Fluorescence intensity changes of constructs from panel A (the color coding is the same as in panel A). At time 0 the promoter was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star the transcription was started by adding ATP, UTP, GTP and heparin. The arrow indicates addition of CTP. (C) Comparison of calculated relative 9 bp duplex stabilities for P27-36 (red), P27-37 (green), with P27 construct (black). (D) Fluorescence intensity changes of constructs from panel C (the color coding is the same as in panel D). At time 0 the DNA construct was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star the transcription was started by adding ATP, UTP, GTP and heparin.

    Journal: Biochemistry

    Article Title: Real-time observation of backtracking by bacterial RNA polymerase

    doi: 10.1021/acs.biochem.5b01184

    Figure Lengend Snippet: (A) Comparison of calculated relative 9 bp duplex stabilities for P27-31 (cyan), P27-32 (pink), P27-33 (blue), P27-34 (green), and P27-35 (red) constructs with P27 construct (black). (B) Fluorescence intensity changes of constructs from panel A (the color coding is the same as in panel A). At time 0 the promoter was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star the transcription was started by adding ATP, UTP, GTP and heparin. The arrow indicates addition of CTP. (C) Comparison of calculated relative 9 bp duplex stabilities for P27-36 (red), P27-37 (green), with P27 construct (black). (D) Fluorescence intensity changes of constructs from panel C (the color coding is the same as in panel D). At time 0 the DNA construct was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star the transcription was started by adding ATP, UTP, GTP and heparin.

    Article Snippet: When transcription was initiated with ATP, UTP and GTP in the presence of GreB, only the fluorescence intensity drop signaling RNAP escape was observed and no fluorescence signal recovery phase associated with backtracking was observed ( ).

    Techniques: Construct, Fluorescence

    (A) Design of fluorescent construct (P54) for real-time detection of RNAP backtracking away from the promoter. The star denotes Cy3 label at +29 position of the nontemplate strand. (B) Fluorescence intensity changes of P54 promoter. At time 0 the P54 was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star the transcription was started by adding ATP, UTP, GTP and heparin (red), ATP, UTP, GTP, heparin, and GreB (green) or ATP, UTP, GTP, CTP and heparin (blue). The arrow denotes time points where the missing NTP (CTP) was added.

    Journal: Biochemistry

    Article Title: Real-time observation of backtracking by bacterial RNA polymerase

    doi: 10.1021/acs.biochem.5b01184

    Figure Lengend Snippet: (A) Design of fluorescent construct (P54) for real-time detection of RNAP backtracking away from the promoter. The star denotes Cy3 label at +29 position of the nontemplate strand. (B) Fluorescence intensity changes of P54 promoter. At time 0 the P54 was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star the transcription was started by adding ATP, UTP, GTP and heparin (red), ATP, UTP, GTP, heparin, and GreB (green) or ATP, UTP, GTP, CTP and heparin (blue). The arrow denotes time points where the missing NTP (CTP) was added.

    Article Snippet: When transcription was initiated with ATP, UTP and GTP in the presence of GreB, only the fluorescence intensity drop signaling RNAP escape was observed and no fluorescence signal recovery phase associated with backtracking was observed ( ).

    Techniques: Construct, Fluorescence

    (A) Design of fluorescent construct (P27 promoter) for real-time detection of RNAP backtracking. The star denotes Cy3 label at the +2 position of the nontemplate strand. (B) Calculated relative duplex stabilities (ratio of duplex free energy of the 9 bp hybrid to the average of free energies of all possible sequence combinations for 9 bp long duplexes) of the 9 bp RNA:DNA hybrids along the sequence of the first 27 transcribed nucleotides of P27. (C) Fluorescence intensity changes of P27. At time 0 the P27 was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star, the transcription was started by adding ATP, UTP, GTP and heparin (grey) or ATP, UTP, GTP, CTP and heparin (black).

    Journal: Biochemistry

    Article Title: Real-time observation of backtracking by bacterial RNA polymerase

    doi: 10.1021/acs.biochem.5b01184

    Figure Lengend Snippet: (A) Design of fluorescent construct (P27 promoter) for real-time detection of RNAP backtracking. The star denotes Cy3 label at the +2 position of the nontemplate strand. (B) Calculated relative duplex stabilities (ratio of duplex free energy of the 9 bp hybrid to the average of free energies of all possible sequence combinations for 9 bp long duplexes) of the 9 bp RNA:DNA hybrids along the sequence of the first 27 transcribed nucleotides of P27. (C) Fluorescence intensity changes of P27. At time 0 the P27 was added to a solution of RNAP in a fluorometer cuvette. At the time point marked by a star, the transcription was started by adding ATP, UTP, GTP and heparin (grey) or ATP, UTP, GTP, CTP and heparin (black).

    Article Snippet: When transcription was initiated with ATP, UTP and GTP in the presence of GreB, only the fluorescence intensity drop signaling RNAP escape was observed and no fluorescence signal recovery phase associated with backtracking was observed ( ).

    Techniques: Construct, Sequencing, Fluorescence

    (A) MMP-sensitive NS and (B) in vivo NIR fluorescence tomographic images of subcutaneous SCC7 tumor-bearing mice after intravenous injection of the NS (Lee et al., 2009 ), © 2009, American Chemical Society.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Nanoparticle-facilitated functional and molecular imaging for the early detection of cancer

    doi: 10.3389/fmolb.2014.00015

    Figure Lengend Snippet: (A) MMP-sensitive NS and (B) in vivo NIR fluorescence tomographic images of subcutaneous SCC7 tumor-bearing mice after intravenous injection of the NS (Lee et al., 2009 ), © 2009, American Chemical Society.

    Article Snippet: In the presence of MMP, the NIR-dye substrate was cleaved, which resulted in NIR fluorescence signal recovery.

    Techniques: In Vivo, Fluorescence, Mouse Assay, Injection

    (A) Schematic diagram of the MMP-sensitive NS. Nanoparticles can deliver multiquenched fluorogenic peptide efficiently to the target site by the EPR effect. When MMPs meet the NS at the site of disease, cleavage of the fluorogenic peptide occurs owing to specific substrate recognition by the MMPs, manifest in the form of pronounced NIR fluorescence signal recovery due to dequenching of the dye. (B) MMP-sensitive NS. Because of the efficient NIR fluorescence quenching ability of the quencher (BHQ-3) and self-quenching of the Cy5.5 dye itself, fluorogenic peptides that are chemically conjugated on the surface of nanoparticles are in the strongly multiquenched state. After cleavage of the substrate by MMPs, NIR fluorescence dyes are released from nanoparticles and fluoresce brightly. (C) Chemical structures of polymeric nanoparticles and MMP-sensitive fluorogenic peptide.

    Journal: Nano letters

    Article Title: Polymeric Nanoparticle-Based Activatable Near-Infrared Nanosensor for Protease Determination In Vivo

    doi: 10.1021/nl902709m

    Figure Lengend Snippet: (A) Schematic diagram of the MMP-sensitive NS. Nanoparticles can deliver multiquenched fluorogenic peptide efficiently to the target site by the EPR effect. When MMPs meet the NS at the site of disease, cleavage of the fluorogenic peptide occurs owing to specific substrate recognition by the MMPs, manifest in the form of pronounced NIR fluorescence signal recovery due to dequenching of the dye. (B) MMP-sensitive NS. Because of the efficient NIR fluorescence quenching ability of the quencher (BHQ-3) and self-quenching of the Cy5.5 dye itself, fluorogenic peptides that are chemically conjugated on the surface of nanoparticles are in the strongly multiquenched state. After cleavage of the substrate by MMPs, NIR fluorescence dyes are released from nanoparticles and fluoresce brightly. (C) Chemical structures of polymeric nanoparticles and MMP-sensitive fluorogenic peptide.

    Article Snippet: When the NS is exposed to the specific MMP of interest, cleavage of the NIR-dye substrate occurs due to specific substrate recognition by the MMPs; this is manifest in the form of a pronounced NIR fluorescence signal recovery due to dequenching of the dye ( ).

    Techniques: Electron Paramagnetic Resonance, Fluorescence