fluorescence quantitative polymerase chain reaction pcr instrument  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr instrument
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence quantitative polymerase chain reaction pcr instrument/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    fluorescence quantitative polymerase chain reaction pcr instrument - by Bioz Stars, 2020-09
    91/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells
    Article Snippet: .. The fluorescence quantitative polymerase chain reaction (PCR) instrument (model: 7900HT) was purchased from Applied Biosystems (Waltham, MA, USA). .. The flow cytometer (model: BD LSR II) was obtained from Becton Dickinson (Franklin, NJ, USA).

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. Main instruments and reagents ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Collection of samples Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Polymerase Chain Reaction:

    Article Title: Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells
    Article Snippet: .. The fluorescence quantitative polymerase chain reaction (PCR) instrument (model: 7900HT) was purchased from Applied Biosystems (Waltham, MA, USA). .. The flow cytometer (model: BD LSR II) was obtained from Becton Dickinson (Franklin, NJ, USA).

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. Main instruments and reagents ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Collection of samples Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    RNA Extraction:

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. Main instruments and reagents ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Collection of samples Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Fluorescence:

    Article Title: Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells
    Article Snippet: .. The fluorescence quantitative polymerase chain reaction (PCR) instrument (model: 7900HT) was purchased from Applied Biosystems (Waltham, MA, USA). .. The flow cytometer (model: BD LSR II) was obtained from Becton Dickinson (Franklin, NJ, USA).

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. Main instruments and reagents ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Collection of samples Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Spectrophotometry:

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. Main instruments and reagents ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Collection of samples Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

    Article Title: Expression of serum AMPD1 in thyroid carcinoma and its clinical significance
    Article Snippet: .. ABI StepOne Plus (Applied Biosystems, Foster City, CA, USA) fluorescence quantitative polymerase chain reaction (PCR) instrument, NanoDrop 2000 spectrophotometer, KH19A desktop high-speed high-performance centrifuge (KAIDA); −80°C low-temperature refrigerator (Thermo Fisher Scientific); AxyGen Total RNA Extraction kit was purchased from Takara Biotechnology Co., Ltd., Dalian, China; reverse transcription kit was from Thermo Fisher Scientific; fluorescent quantitative PCR kit was from Takara Biotechnology Co., Ltd. .. Peripheral blood (5 ml) was extracted from patients with PTC and volunteers in normal control group before operation and placed into an ethylenediaminetetraacetic acid-k (EDTA-k) anticoagulant tube, followed by centrifugation at 3500 × g for 5 min.

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    Thermo Fisher real time pcr instrument
    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the <t>ABI</t> 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per <t>PCR</t> reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Real Time Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr instrument/product/Thermo Fisher
    Average 99 stars, based on 92 article reviews
    Price from $9.99 to $1999.99
    real time pcr instrument - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher real time pcr rt qpcr
    The hAgo2-miRNA complex transferred by MPs into Plasmodium parasite in vitro and in vivo. ( A ) Annotations of high-throughput sequencing data of small RNAs bound by hAgo2. The read counts of each RNA class are listed in round brackets. ( B ) Fluorescence in situ hybridization detection of miR-451 in iRBCs. Black arrows in light fields indicate the parasites. miR-451 was labeled with 5′ carboxyfluorescein (FAM)/scramble probes (green), and the parasite nuclei were labeled with DAPI (blue). ( C ) <t>RT-qPCR</t> (left top) and stem-loop <t>RT-PCR</t> (left bottom) analysis of miR-451, miR-486 and miR-181a within nMPs and iMPs; RT-qPCR (right top) and northern blot (right bottom) analysis of these miRNAs in nRBCs and iRBCs. ( D ) RT-qPCR analysis of miR-451 in iRBCs treated with nMPs (left) and iMPs (right). Synchronized ring stage iRBCs were incubated with different concentrations of nMPs (1 ×, 10 ×, 100 ×) or iMPs at separate times (16, 32 h). The level of miR-451 in iRBCs/nRBCs was given as a relative value of 1.0 ( n =3). ( E ) RT-qPCR analysis of Mmu-miR-451 in the nRBCs (Mmu-nRBCs) and iRBCs of P. yoelii -infected mice ( P.y. iRBCs) at different parasitemia ratios (1%±0.5% and 10%±5%) (i.e., three mice in each group) ( n =3).
    Real Time Pcr Rt Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr rt qpcr/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    real time pcr rt qpcr - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Thermo Fisher real time qpcr
    Depletion of z-miR-200- class in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), or injected with control MO, or injected with anti- z-miR-200 class (right panels) MO. The control MO did not cause significant alterations. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing normal embryo morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Proportions of embryos showing either placode disorganization, olfactory axon mistargeting, or both (last bars) after injection of control or z-miR-200 class MOs. e. Quantification of endogenous miR-200 class in embryos injected with control or anti- z-miR-200 MOs, by Real-Time <t>qPCR.</t> Results show a significant decrease of miR-200 abundance in the injected embryos. f. Quantification of endogenous z-foxg1 <t>mRNA</t> in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-200 causes a significant increase of z-foxg1 mRNA abundance. g. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-200 class MOs, relative to control MO injected embryos, set = 1. The abundance of z-hoxA7a and abundance of z-hoxA10b were also determined and used as in Fig. 5 .
    Real Time Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1745 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time qpcr/product/Thermo Fisher
    Average 99 stars, based on 1745 article reviews
    Price from $9.99 to $1999.99
    real time qpcr - by Bioz Stars, 2020-09
    99/100 stars
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    Image Search Results


    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    The hAgo2-miRNA complex transferred by MPs into Plasmodium parasite in vitro and in vivo. ( A ) Annotations of high-throughput sequencing data of small RNAs bound by hAgo2. The read counts of each RNA class are listed in round brackets. ( B ) Fluorescence in situ hybridization detection of miR-451 in iRBCs. Black arrows in light fields indicate the parasites. miR-451 was labeled with 5′ carboxyfluorescein (FAM)/scramble probes (green), and the parasite nuclei were labeled with DAPI (blue). ( C ) RT-qPCR (left top) and stem-loop RT-PCR (left bottom) analysis of miR-451, miR-486 and miR-181a within nMPs and iMPs; RT-qPCR (right top) and northern blot (right bottom) analysis of these miRNAs in nRBCs and iRBCs. ( D ) RT-qPCR analysis of miR-451 in iRBCs treated with nMPs (left) and iMPs (right). Synchronized ring stage iRBCs were incubated with different concentrations of nMPs (1 ×, 10 ×, 100 ×) or iMPs at separate times (16, 32 h). The level of miR-451 in iRBCs/nRBCs was given as a relative value of 1.0 ( n =3). ( E ) RT-qPCR analysis of Mmu-miR-451 in the nRBCs (Mmu-nRBCs) and iRBCs of P. yoelii -infected mice ( P.y. iRBCs) at different parasitemia ratios (1%±0.5% and 10%±5%) (i.e., three mice in each group) ( n =3).

    Journal: Emerging Microbes & Infections

    Article Title: Red blood cells release microparticles containing human argonaute 2 and miRNAs to target genes of Plasmodium falciparum

    doi: 10.1038/emi.2017.63

    Figure Lengend Snippet: The hAgo2-miRNA complex transferred by MPs into Plasmodium parasite in vitro and in vivo. ( A ) Annotations of high-throughput sequencing data of small RNAs bound by hAgo2. The read counts of each RNA class are listed in round brackets. ( B ) Fluorescence in situ hybridization detection of miR-451 in iRBCs. Black arrows in light fields indicate the parasites. miR-451 was labeled with 5′ carboxyfluorescein (FAM)/scramble probes (green), and the parasite nuclei were labeled with DAPI (blue). ( C ) RT-qPCR (left top) and stem-loop RT-PCR (left bottom) analysis of miR-451, miR-486 and miR-181a within nMPs and iMPs; RT-qPCR (right top) and northern blot (right bottom) analysis of these miRNAs in nRBCs and iRBCs. ( D ) RT-qPCR analysis of miR-451 in iRBCs treated with nMPs (left) and iMPs (right). Synchronized ring stage iRBCs were incubated with different concentrations of nMPs (1 ×, 10 ×, 100 ×) or iMPs at separate times (16, 32 h). The level of miR-451 in iRBCs/nRBCs was given as a relative value of 1.0 ( n =3). ( E ) RT-qPCR analysis of Mmu-miR-451 in the nRBCs (Mmu-nRBCs) and iRBCs of P. yoelii -infected mice ( P.y. iRBCs) at different parasitemia ratios (1%±0.5% and 10%±5%) (i.e., three mice in each group) ( n =3).

    Article Snippet: For detection of miRNAs by quantitative real-time PCR (RT-qPCR), synthesized Arabidopsis thaliana miR-404 RNA oligos (ath-miR-404) were added to counted cells (2 nmol/108 RBCs) before total RNA extraction and were used as an exogenous reference for data normalization.

    Techniques: In Vitro, In Vivo, Next-Generation Sequencing, Fluorescence, In Situ Hybridization, Labeling, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Northern Blot, Incubation, Infection, Mouse Assay

    Depletion of z-miR-200- class in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), or injected with control MO, or injected with anti- z-miR-200 class (right panels) MO. The control MO did not cause significant alterations. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing normal embryo morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Proportions of embryos showing either placode disorganization, olfactory axon mistargeting, or both (last bars) after injection of control or z-miR-200 class MOs. e. Quantification of endogenous miR-200 class in embryos injected with control or anti- z-miR-200 MOs, by Real-Time qPCR. Results show a significant decrease of miR-200 abundance in the injected embryos. f. Quantification of endogenous z-foxg1 mRNA in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-200 causes a significant increase of z-foxg1 mRNA abundance. g. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-200 class MOs, relative to control MO injected embryos, set = 1. The abundance of z-hoxA7a and abundance of z-hoxA10b were also determined and used as in Fig. 5 .

    Journal: Molecular and Cellular Neurosciences

    Article Title: The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system

    doi: 10.1016/j.mcn.2015.04.007

    Figure Lengend Snippet: Depletion of z-miR-200- class in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), or injected with control MO, or injected with anti- z-miR-200 class (right panels) MO. The control MO did not cause significant alterations. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing normal embryo morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Proportions of embryos showing either placode disorganization, olfactory axon mistargeting, or both (last bars) after injection of control or z-miR-200 class MOs. e. Quantification of endogenous miR-200 class in embryos injected with control or anti- z-miR-200 MOs, by Real-Time qPCR. Results show a significant decrease of miR-200 abundance in the injected embryos. f. Quantification of endogenous z-foxg1 mRNA in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-200 causes a significant increase of z-foxg1 mRNA abundance. g. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-200 class MOs, relative to control MO injected embryos, set = 1. The abundance of z-hoxA7a and abundance of z-hoxA10b were also determined and used as in Fig. 5 .

    Article Snippet: 2.7 Real-Time qPCR analysis for coding mRNAs Relative mRNA abundance was determined by Real-Time qPCR.

    Techniques: Fluorescence, Injection, Real-time Polymerase Chain Reaction

    Depletion of z-miR-9 in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos injected with control (top panels) or anti- z-miR-9 (bottom panels) MOs. The control MO did not cause any significant alteration. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing a normal embryonic morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Quantification of endogenous miR-9 in zebrafish embryos injected with control or anti- z-miR-9 MOs, by Real-Time qPCR. Results show efficient depletion. e. Quantification of endogenous z-foxg1 mRNAs in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-9 causes a significant increase of the z-foxg1 mRNA. f. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-9 MOs, relative to control MO injected embryos. Samples were collected 72 hpf. Results are shown relative to the control injected samples, made = 1. The relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs were determined, to monitor progression of development and exclude a generalized delay.

    Journal: Molecular and Cellular Neurosciences

    Article Title: The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system

    doi: 10.1016/j.mcn.2015.04.007

    Figure Lengend Snippet: Depletion of z-miR-9 in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos injected with control (top panels) or anti- z-miR-9 (bottom panels) MOs. The control MO did not cause any significant alteration. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing a normal embryonic morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Quantification of endogenous miR-9 in zebrafish embryos injected with control or anti- z-miR-9 MOs, by Real-Time qPCR. Results show efficient depletion. e. Quantification of endogenous z-foxg1 mRNAs in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-9 causes a significant increase of the z-foxg1 mRNA. f. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-9 MOs, relative to control MO injected embryos. Samples were collected 72 hpf. Results are shown relative to the control injected samples, made = 1. The relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs were determined, to monitor progression of development and exclude a generalized delay.

    Article Snippet: 2.7 Real-Time qPCR analysis for coding mRNAs Relative mRNA abundance was determined by Real-Time qPCR.

    Techniques: Fluorescence, Injection, Real-time Polymerase Chain Reaction

    Depletion of z-dlx5a in zebrafish embryos causes delayed olfactory differentiation. a. Confocal stacked images of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), injected with a control MO (right), or injected with anti- z-dlx5a MO (bottom panels), taken at 72 hpf. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing an overall normal embryonic morphology and growth rate in the injected embryos, compared to the non-injected ones. c. Percentages of embryos showing YFP or CFP fluorescence, over the total of examined ones, comparing not-injected, control injected and MO injected ones. d. RT-PCR analysis on RNA extracted from anti- z-dlx5a MO-treated and control embryos, showing that the MO efficiently generates an inactive splice-variant form of the endogenous mRNA. A scheme of the z-dlx5a gene (Ex1–Ex2–Ex3), the positions of the z-dlx5a MO and the position of the PCR primers (A–D) are reported on the left. e. (on the left) Quantification of the olfactory differentiation phenotype by Real-Time qPCR for differentiation-related mRNAs in a sample of the embryonic heads of MO-injected embryos (grey bars). Embryos injected with control MO were used for comparison (open bars). Normalization is carried out relative to control samples, made = 1. (on the right) Relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs in whole embryos injected with z-dlx5a MO (grey bars), to monitor developmental progression and exclude a generalized delay.

    Journal: Molecular and Cellular Neurosciences

    Article Title: The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system

    doi: 10.1016/j.mcn.2015.04.007

    Figure Lengend Snippet: Depletion of z-dlx5a in zebrafish embryos causes delayed olfactory differentiation. a. Confocal stacked images of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), injected with a control MO (right), or injected with anti- z-dlx5a MO (bottom panels), taken at 72 hpf. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing an overall normal embryonic morphology and growth rate in the injected embryos, compared to the non-injected ones. c. Percentages of embryos showing YFP or CFP fluorescence, over the total of examined ones, comparing not-injected, control injected and MO injected ones. d. RT-PCR analysis on RNA extracted from anti- z-dlx5a MO-treated and control embryos, showing that the MO efficiently generates an inactive splice-variant form of the endogenous mRNA. A scheme of the z-dlx5a gene (Ex1–Ex2–Ex3), the positions of the z-dlx5a MO and the position of the PCR primers (A–D) are reported on the left. e. (on the left) Quantification of the olfactory differentiation phenotype by Real-Time qPCR for differentiation-related mRNAs in a sample of the embryonic heads of MO-injected embryos (grey bars). Embryos injected with control MO were used for comparison (open bars). Normalization is carried out relative to control samples, made = 1. (on the right) Relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs in whole embryos injected with z-dlx5a MO (grey bars), to monitor developmental progression and exclude a generalized delay.

    Article Snippet: 2.7 Real-Time qPCR analysis for coding mRNAs Relative mRNA abundance was determined by Real-Time qPCR.

    Techniques: Fluorescence, Injection, Reverse Transcription Polymerase Chain Reaction, Variant Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Differential expression of microRNAs (miRNAs) in the corpus callosum of mice during experimental demyelination and remyelination. Using microarray and validation by quantitative PCR (qPCR), three miRNAs, miR-146a (A) , miR-193a (B) and miR-181b (C) were differentially regulated in response to CPZ exposure in the corpus callosum. * p

    Journal: Frontiers in Immunology

    Article Title: Experimental Demyelination and Axonal Loss Are Reduced in MicroRNA-146a Deficient Mice

    doi: 10.3389/fimmu.2018.00490

    Figure Lengend Snippet: Differential expression of microRNAs (miRNAs) in the corpus callosum of mice during experimental demyelination and remyelination. Using microarray and validation by quantitative PCR (qPCR), three miRNAs, miR-146a (A) , miR-193a (B) and miR-181b (C) were differentially regulated in response to CPZ exposure in the corpus callosum. * p

    Article Snippet: Extraction of Whole RNA and Quantitative PCR (qPCR)For the removal of the corpus callosum, the brains were removed from the skull, immediately frozen, and cut into coronal serial sections (section thickness 200 µm).

    Techniques: Expressing, Mouse Assay, Microarray, Real-time Polymerase Chain Reaction