fluorescence labeled oligos  (Thermo Fisher)


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    Name:
    Oligo dT Primer 50 µM
    Description:
    These are the same Ambion primers that are currently included in the RETROscript Kit SKU AM1710 They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript Kit
    Catalog Number:
    am5730g
    Price:
    None
    Applications:
    PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR
    Category:
    Oligos Primers Probes Nucleotides
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    Structured Review

    Thermo Fisher fluorescence labeled oligos
    These are the same Ambion primers that are currently included in the RETROscript Kit SKU AM1710 They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript Kit
    https://www.bioz.com/result/fluorescence labeled oligos/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescence labeled oligos - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: A redox mechanism underlying nucleolar stress sensing by nucleophosmin
    Article Snippet: .. Plasmids DNA and siRNA oligo were transfected into cells with Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: Glucocorticoid impairs cell-cell communication by autophagy-mediated degradation of connexin 43 in osteocytes
    Article Snippet: .. Transfection MLO-Y4 cells (1×105 /well) were plated in type-I collagen coated six-well culture plates, and then transfected with various concentrations (12.5nM, 25nM, 50nM and 100nM) of Atg5 siRNA oligo cocktail (Stealth siRNAs: MSS247019, MSS247020, MSS247021, from Thermo Fisher® , USA) or negative control using Lipofectamine® 3000 Transfection Reagent (Invitrogen® , USA) following the manufacturer's protocol. .. Confocal microscopy MLO-Y4 cells cultured on glass coverslips and calvarial primary osteocytes ex vivo cultured in 96-wells plates were fixed for 15mins and and 2hrs at RT with 4% PFA respectively.

    Article Title: miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors
    Article Snippet: .. Cell Transfection Cells were transfected with 30 mM Ambion pre-miR-411 precursor oligos (Life Technologies) using Lipofectamine 2000 (Life Technologies) following the manufacture protocol. .. Briefly, 4×103 /cm2 C2 C12 cells were plated in growth medium one day before the transfection.

    Article Title: Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells
    Article Snippet: .. RNAi was performed by the transfection of siRNA oligos using the Lipofectamine™ RNAiMAX transfection reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions. ..

    Amplification:

    Article Title: iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture
    Article Snippet: .. We then amplified the oligos in each well by real-time PCR using the SYBR Select Master Mix (Thermo Fisher Scientific, cat. no. 4472913). .. We designed PCR primers that would anneal to the F and R adapters specific for each oligo in the pool, and incorporate the C adapter and T7 promoter sequence, on the 5’ side of the F and R adapters, respectively.

    Synthesized:

    Article Title: Human RAD9 checkpoint control/proapoptotic protein can activate transcription of p21
    Article Snippet: .. Complementary oligos containing the p53-binding site were synthesized commercially (Invitrogen) and annealed in buffer (100 mM NaCl/5 mM Tris·Cl, pH 7.5/10 mM MgCl2 /0.02 mM EDTA/1 mM DTT). .. These probes were end-labeled with [γ-32 P]ATP by using T4 polynucleotide kinase (New England Biolabs).

    Magnetic Beads:

    Article Title: Transposable elements become active and mobile in the genomes of aging mammalian somatic tissues
    Article Snippet: .. Polyadenylated mRNA was prepared using two consecutive purifications with oligo-dT magnetic beads, following the mRNA Direct Dynabeads Kit protocol provided by the manufacturer (Invitrogen). .. The eluted mRNA was quantified using the Qubit 2.0 RNA HS Assay Kit (Invitrogen).

    Negative Control:

    Article Title: Glucocorticoid impairs cell-cell communication by autophagy-mediated degradation of connexin 43 in osteocytes
    Article Snippet: .. Transfection MLO-Y4 cells (1×105 /well) were plated in type-I collagen coated six-well culture plates, and then transfected with various concentrations (12.5nM, 25nM, 50nM and 100nM) of Atg5 siRNA oligo cocktail (Stealth siRNAs: MSS247019, MSS247020, MSS247021, from Thermo Fisher® , USA) or negative control using Lipofectamine® 3000 Transfection Reagent (Invitrogen® , USA) following the manufacturer's protocol. .. Confocal microscopy MLO-Y4 cells cultured on glass coverslips and calvarial primary osteocytes ex vivo cultured in 96-wells plates were fixed for 15mins and and 2hrs at RT with 4% PFA respectively.

    Real-time Polymerase Chain Reaction:

    Article Title: iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture
    Article Snippet: .. We then amplified the oligos in each well by real-time PCR using the SYBR Select Master Mix (Thermo Fisher Scientific, cat. no. 4472913). .. We designed PCR primers that would anneal to the F and R adapters specific for each oligo in the pool, and incorporate the C adapter and T7 promoter sequence, on the 5’ side of the F and R adapters, respectively.

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  • 99
    Thermo Fisher block it fluorescent oligo
    Block It Fluorescent Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/block it fluorescent oligo/product/Thermo Fisher
    Average 99 stars, based on 130 article reviews
    Price from $9.99 to $1999.99
    block it fluorescent oligo - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Thermo Fisher fluorescence polarization assays 5 fluorescein labeled rna oligos
    ISAV-NP <t>RNA</t> binding. (A–C) RNA binding affinity measurements for the wt NP (A), ΔN111 (B) and ΔC16 (C). FA was performed using three RNA <t>oligos</t> each containing 20, 24, and 28 nts. The binding curves are plotted using the same protein concentration range. For the ΔC16 mutant, an inset is added in (C) to provide a more spread-out view at lower protein concentrations. Fully saturated curves for the 20-nt RNA are shown in Fig. S3 in Supporting Information. (D–G) RNA binding stoichiometry measurements for the wt NP and the ΔLOOP monomeric mutant. NP:RNA complexes assembled in each experiments are schematically shown with blue handcuffs for NP dimers and grey curves for RNA molecules.
    Fluorescence Polarization Assays 5 Fluorescein Labeled Rna Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence polarization assays 5 fluorescein labeled rna oligos/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescence polarization assays 5 fluorescein labeled rna oligos - by Bioz Stars, 2020-08
    85/100 stars
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    Image Search Results


    ISAV-NP RNA binding. (A–C) RNA binding affinity measurements for the wt NP (A), ΔN111 (B) and ΔC16 (C). FA was performed using three RNA oligos each containing 20, 24, and 28 nts. The binding curves are plotted using the same protein concentration range. For the ΔC16 mutant, an inset is added in (C) to provide a more spread-out view at lower protein concentrations. Fully saturated curves for the 20-nt RNA are shown in Fig. S3 in Supporting Information. (D–G) RNA binding stoichiometry measurements for the wt NP and the ΔLOOP monomeric mutant. NP:RNA complexes assembled in each experiments are schematically shown with blue handcuffs for NP dimers and grey curves for RNA molecules.

    Journal: PLoS Pathogens

    Article Title: The Crystal Structure and RNA-Binding of an Orthomyxovirus Nucleoprotein

    doi: 10.1371/journal.ppat.1003624

    Figure Lengend Snippet: ISAV-NP RNA binding. (A–C) RNA binding affinity measurements for the wt NP (A), ΔN111 (B) and ΔC16 (C). FA was performed using three RNA oligos each containing 20, 24, and 28 nts. The binding curves are plotted using the same protein concentration range. For the ΔC16 mutant, an inset is added in (C) to provide a more spread-out view at lower protein concentrations. Fully saturated curves for the 20-nt RNA are shown in Fig. S3 in Supporting Information. (D–G) RNA binding stoichiometry measurements for the wt NP and the ΔLOOP monomeric mutant. NP:RNA complexes assembled in each experiments are schematically shown with blue handcuffs for NP dimers and grey curves for RNA molecules.

    Article Snippet: Fluorescence polarization assays 5′-fluorescein-labeled RNA oligos were purchased from Thermo Fisher Scientific and Sigma-Aldrich.

    Techniques: RNA Binding Assay, Binding Assay, Protein Concentration, Mutagenesis