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Leica Microsystems fluorescence inverted microscopy
Fluorescence Inverted Microscopy, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence inverted microscopy/product/Leica Microsystems
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fluorescence inverted microscopy - by Bioz Stars, 2020-04
89/100 stars

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Related Articles

MTT Assay:

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: Cell viability assays LN229 or T98G cell viability after V-ATPase inhibition by selective drugs was analyzed after 48 h by measuring mitochondrial function through the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) as reported [ ]. .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems).

Flow Cytometry:

Article Title: Podocyte Expression of Membrane Transporters Involved in Puromycin Aminonucleoside-Mediated Injury
Article Snippet: After incubation the cells were washed in HBSS buffer and observed by fluorescence inverted microscopy (Leica Microsystems GmbH, Germany). .. To confirm the cellular uptake of Penicillin, we performed a flow cytometry analysis.

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: Alternatively, cells were stained with Annexin V/PI using the FITC Annexin V Apoptosis Detection Kit (BD Bioscience, San Diego, CA, USA), evaluated by multiparametric flow cytometry and analyzed using FlowJo software. .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems).

Fluorescence:

Article Title: Podocyte Expression of Membrane Transporters Involved in Puromycin Aminonucleoside-Mediated Injury
Article Snippet: .. After incubation the cells were washed in HBSS buffer and observed by fluorescence inverted microscopy (Leica Microsystems GmbH, Germany). .. To confirm the cellular uptake of Penicillin, we performed a flow cytometry analysis.

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems). .. Detection of effectors caspase activation was performed incubating live cultures with the CellEvent Caspase-3/7 Green probe (Life Technologies) and signal (at 503nm) or bright-field images were acquired using a fluorescence microscope.

Synthesized:

Article Title: Podocyte Expression of Membrane Transporters Involved in Puromycin Aminonucleoside-Mediated Injury
Article Snippet: Bocillin TM 650-655 Penicillin is a synthesized compound from Penicillin V and the Bodipy 650/655 dye. .. After incubation the cells were washed in HBSS buffer and observed by fluorescence inverted microscopy (Leica Microsystems GmbH, Germany).

Cytometry:

Article Title: Podocyte Expression of Membrane Transporters Involved in Puromycin Aminonucleoside-Mediated Injury
Article Snippet: After incubation the cells were washed in HBSS buffer and observed by fluorescence inverted microscopy (Leica Microsystems GmbH, Germany). .. To confirm the cellular uptake of Penicillin, we performed a flow cytometry analysis.

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: Alternatively, cells were stained with Annexin V/PI using the FITC Annexin V Apoptosis Detection Kit (BD Bioscience, San Diego, CA, USA), evaluated by multiparametric flow cytometry and analyzed using FlowJo software. .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems).

Microscopy:

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems). .. Detection of effectors caspase activation was performed incubating live cultures with the CellEvent Caspase-3/7 Green probe (Life Technologies) and signal (at 503nm) or bright-field images were acquired using a fluorescence microscope.

Activation Assay:

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems). .. Detection of effectors caspase activation was performed incubating live cultures with the CellEvent Caspase-3/7 Green probe (Life Technologies) and signal (at 503nm) or bright-field images were acquired using a fluorescence microscope.

Incubation:

Article Title: Podocyte Expression of Membrane Transporters Involved in Puromycin Aminonucleoside-Mediated Injury
Article Snippet: .. After incubation the cells were washed in HBSS buffer and observed by fluorescence inverted microscopy (Leica Microsystems GmbH, Germany). .. To confirm the cellular uptake of Penicillin, we performed a flow cytometry analysis.

Transfection:

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: Cell cycle transitions and quantification of hypodiploid DNA content (i.e. sub-G1 fraction) were determined in transfected or drug treated cultures after 48 h, by propidium iodide staining and flow cytometry, as described [ ]. .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems).

Inverted Microscopy:

Article Title: Podocyte Expression of Membrane Transporters Involved in Puromycin Aminonucleoside-Mediated Injury
Article Snippet: .. After incubation the cells were washed in HBSS buffer and observed by fluorescence inverted microscopy (Leica Microsystems GmbH, Germany). .. To confirm the cellular uptake of Penicillin, we performed a flow cytometry analysis.

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems). .. Detection of effectors caspase activation was performed incubating live cultures with the CellEvent Caspase-3/7 Green probe (Life Technologies) and signal (at 503nm) or bright-field images were acquired using a fluorescence microscope.

Staining:

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: Alternatively, cells were stained with Annexin V/PI using the FITC Annexin V Apoptosis Detection Kit (BD Bioscience, San Diego, CA, USA), evaluated by multiparametric flow cytometry and analyzed using FlowJo software. .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems).

Inhibition:

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: Cell viability assays LN229 or T98G cell viability after V-ATPase inhibition by selective drugs was analyzed after 48 h by measuring mitochondrial function through the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) as reported [ ]. .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems).

Software:

Article Title: The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
Article Snippet: Alternatively, cells were stained with Annexin V/PI using the FITC Annexin V Apoptosis Detection Kit (BD Bioscience, San Diego, CA, USA), evaluated by multiparametric flow cytometry and analyzed using FlowJo software. .. For GBM neurospheres the FITC Annexin V Fluorescence Microscopy Kit (BD Bioscience) was used and signal intensity was acquired using a fluorescence inverted microscopy (DMI4000B, Leica Microsystems).

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  • 93
    Leica Microsystems leica dmi inverted fluorescent microscope
    Encapsulated islet survival and insulin secretion. (A) Control and encapsulated islets (PEG-VS hydrogel at pH=8.0) were cultured for 4 d in cCMRL-1066 medium containing 10 mM glucose. After a 4 d incubation, brightfield images of islets were acquired using a 10X objective in a <t>Leica</t> <t>DMI</t> inverted fluorescent microscope. (B) Control and encapsulated islets (PEG-VS hydrogels formed at pH 7.4 or 8.0) were pre-incubated in cCMRL-1066 containing 5.6 mM glucose for 30 min, washed, and incubated for additional 1 h in cCMRL-1066 containing 5.6 mL or 20 mM glucose. Supernatants were assayed for insulin content by RIA. Data show the average of triplicates of 6 independent experiments. Values are presented as the means ± SEM. For Figure 2B, two-way ANOVA and Tukey’s multiple comparison test was performed and significant differences are indicated by asterisks (n=6; **p
    Leica Dmi Inverted Fluorescent Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leica dmi inverted fluorescent microscope/product/Leica Microsystems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leica dmi inverted fluorescent microscope - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    Leica Microsystems leica dm ire2 inverted fluorescence microscope
    P. aeruginosa PAO1 biofilm phenotypes as affected by cassipourol or β-sitosterol or α-amyrin. P. aeruginosa PAO1 cells were incubated statically at 37 °C for 24 h for biofilm formation in presence of DMSO 1%, or cassipourol (CAS) or β-sitosterol (SIT) or α-amyrin (AMY) at 100 µM. Cells were visualized after staining with SYTO-9 (green fluorescence for living bacteria) and propidium iodide (red fluorescence for dead bacteria) furnished in the LIVE/DEAD Bac Light kit. Fluorescence microscopy was achieved by using a <t>Leica</t> DM <t>IRE2</t> inverted fluorescence microscope using a 40× objective lens and images were false-colored and assembled using Adobe Photoshop.
    Leica Dm Ire2 Inverted Fluorescence Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leica dm ire2 inverted fluorescence microscope/product/Leica Microsystems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leica dm ire2 inverted fluorescence microscope - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    91
    Leica Microsystems leica dm irbe inverted fluorescence microscope
    Gonococcal binding and internalization to iMDDCs. A ) Immature MDDCs were incubated with FITC-labeled gonococcal strains N302, N303, N309, and N496, or E.coli DH5α bacteria at an MOI of 10 for 30 min at RT. Bacterial binding was determined by measuring the percentage of cells that bound FITC-labeled bacteria using flow cytometric analysis. The percentage of cells with bound FITC-labeled bacteria is indicated in each condition. B) Bacterial binding to iMDDCs is not mediated by DC-SIGN on the cell surface. Mannan blocking assays were carried out site-by-site with the bacterial binding tests. The percentage of cells with bound FITC-labeled bacteria is indicated in each condition in the presence of mannan at 20 µg/ml. C) Internalization of gonococcal strains and E.coli DH5α into iMDDCs. Immature MDDCs were allowed to adhere onto coverslips pre-coated with 0.2% gelatin. These cells were pulsed with gonococcal strains or E.coli DH5α (MOI = 100) prelabeled with Texas red-X-succinimidyl ester at 37 o C for 1 h. Extracellular bacteria were then labeled with the polyclonal anti-gonococcal serum, followed by a staining with a BODIPY-FL-conjugated secondary Ab. Immature MDDCs were then permeabilized with 0.4% Triton X-100 and stained with Phalloidin-FITC. Intracellular (red) versus extracellular (yellow) bacteria with MDDCs (green) were then distinguished by visualization with a <t>Leica</t> <t>DM-IRBE</t> inverted fluorescence microscope. The magnification for all conditions is 40.
    Leica Dm Irbe Inverted Fluorescence Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leica dm irbe inverted fluorescence microscope/product/Leica Microsystems
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    leica dm irbe inverted fluorescence microscope - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    Encapsulated islet survival and insulin secretion. (A) Control and encapsulated islets (PEG-VS hydrogel at pH=8.0) were cultured for 4 d in cCMRL-1066 medium containing 10 mM glucose. After a 4 d incubation, brightfield images of islets were acquired using a 10X objective in a Leica DMI inverted fluorescent microscope. (B) Control and encapsulated islets (PEG-VS hydrogels formed at pH 7.4 or 8.0) were pre-incubated in cCMRL-1066 containing 5.6 mM glucose for 30 min, washed, and incubated for additional 1 h in cCMRL-1066 containing 5.6 mL or 20 mM glucose. Supernatants were assayed for insulin content by RIA. Data show the average of triplicates of 6 independent experiments. Values are presented as the means ± SEM. For Figure 2B, two-way ANOVA and Tukey’s multiple comparison test was performed and significant differences are indicated by asterisks (n=6; **p

    Journal: Biomedical physics & engineering express

    Article Title: Injectable Polyethylene Glycol Hydrogel for Islet Encapsulation: an in vitro and in vivo Characterization

    doi: 10.1088/2057-1976/aa742b

    Figure Lengend Snippet: Encapsulated islet survival and insulin secretion. (A) Control and encapsulated islets (PEG-VS hydrogel at pH=8.0) were cultured for 4 d in cCMRL-1066 medium containing 10 mM glucose. After a 4 d incubation, brightfield images of islets were acquired using a 10X objective in a Leica DMI inverted fluorescent microscope. (B) Control and encapsulated islets (PEG-VS hydrogels formed at pH 7.4 or 8.0) were pre-incubated in cCMRL-1066 containing 5.6 mM glucose for 30 min, washed, and incubated for additional 1 h in cCMRL-1066 containing 5.6 mL or 20 mM glucose. Supernatants were assayed for insulin content by RIA. Data show the average of triplicates of 6 independent experiments. Values are presented as the means ± SEM. For Figure 2B, two-way ANOVA and Tukey’s multiple comparison test was performed and significant differences are indicated by asterisks (n=6; **p

    Article Snippet: Brightfield images of islets cultured for 4 d were acquired using a 10X objective in a Leica DMI inverted fluorescent microscope (Leica Microsystems Inc., Buffalo Grove, IL).

    Techniques: Cell Culture, Incubation, Microscopy

    Frozen sectioning, Hematoxylin staining, and immunohistochemistry of hydrogels after retrieval from mice. (A) Two wk post-injection hydrogels were retrieved from the peritoneal cavity. A hydrogel with an irregular shape conforming to the space available at the injection site (a) of a mouse is shown. Images of two retrieved hydrogels (b) and (c) show tissues and blood vessels attached on the surface. (B) Images of Hematoxylin-stained hydrogel sections were captured using a 5X objective in a Leica DMI inverted fluorescent microscope. The inset shows 3-fold magnification of the designated area. (C) For immunohistochemistry, hydrogel sections were immunostained with Alexa fluor 647 anti-mouse F4/80 antibody for murine macrophages and DAPI for nuclear staining. Fluorescent images were obtained using a 40X objective in an Olympus FluoView confocal microscope.

    Journal: Biomedical physics & engineering express

    Article Title: Injectable Polyethylene Glycol Hydrogel for Islet Encapsulation: an in vitro and in vivo Characterization

    doi: 10.1088/2057-1976/aa742b

    Figure Lengend Snippet: Frozen sectioning, Hematoxylin staining, and immunohistochemistry of hydrogels after retrieval from mice. (A) Two wk post-injection hydrogels were retrieved from the peritoneal cavity. A hydrogel with an irregular shape conforming to the space available at the injection site (a) of a mouse is shown. Images of two retrieved hydrogels (b) and (c) show tissues and blood vessels attached on the surface. (B) Images of Hematoxylin-stained hydrogel sections were captured using a 5X objective in a Leica DMI inverted fluorescent microscope. The inset shows 3-fold magnification of the designated area. (C) For immunohistochemistry, hydrogel sections were immunostained with Alexa fluor 647 anti-mouse F4/80 antibody for murine macrophages and DAPI for nuclear staining. Fluorescent images were obtained using a 40X objective in an Olympus FluoView confocal microscope.

    Article Snippet: Brightfield images of islets cultured for 4 d were acquired using a 10X objective in a Leica DMI inverted fluorescent microscope (Leica Microsystems Inc., Buffalo Grove, IL).

    Techniques: Staining, Immunohistochemistry, Mouse Assay, Injection, Microscopy

    P. aeruginosa PAO1 biofilm phenotypes as affected by cassipourol or β-sitosterol or α-amyrin. P. aeruginosa PAO1 cells were incubated statically at 37 °C for 24 h for biofilm formation in presence of DMSO 1%, or cassipourol (CAS) or β-sitosterol (SIT) or α-amyrin (AMY) at 100 µM. Cells were visualized after staining with SYTO-9 (green fluorescence for living bacteria) and propidium iodide (red fluorescence for dead bacteria) furnished in the LIVE/DEAD Bac Light kit. Fluorescence microscopy was achieved by using a Leica DM IRE2 inverted fluorescence microscope using a 40× objective lens and images were false-colored and assembled using Adobe Photoshop.

    Journal: International Journal of Molecular Sciences

    Article Title: Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

    doi: 10.3390/ijms18061270

    Figure Lengend Snippet: P. aeruginosa PAO1 biofilm phenotypes as affected by cassipourol or β-sitosterol or α-amyrin. P. aeruginosa PAO1 cells were incubated statically at 37 °C for 24 h for biofilm formation in presence of DMSO 1%, or cassipourol (CAS) or β-sitosterol (SIT) or α-amyrin (AMY) at 100 µM. Cells were visualized after staining with SYTO-9 (green fluorescence for living bacteria) and propidium iodide (red fluorescence for dead bacteria) furnished in the LIVE/DEAD Bac Light kit. Fluorescence microscopy was achieved by using a Leica DM IRE2 inverted fluorescence microscope using a 40× objective lens and images were false-colored and assembled using Adobe Photoshop.

    Article Snippet: Biofilms were incubated for 15 min and P. aeruginosa PAO1 cells were examined using a Leica DM IRE2 inverted fluorescence microscope coupled to a CCD camera (Leica DC350 FX, Leica Microsystems Inc., Bannockburn, IL, USA) and equipped with FITC and Texas red filters.

    Techniques: Incubation, Staining, Fluorescence, BAC Assay, Microscopy

    Gonococcal binding and internalization to iMDDCs. A ) Immature MDDCs were incubated with FITC-labeled gonococcal strains N302, N303, N309, and N496, or E.coli DH5α bacteria at an MOI of 10 for 30 min at RT. Bacterial binding was determined by measuring the percentage of cells that bound FITC-labeled bacteria using flow cytometric analysis. The percentage of cells with bound FITC-labeled bacteria is indicated in each condition. B) Bacterial binding to iMDDCs is not mediated by DC-SIGN on the cell surface. Mannan blocking assays were carried out site-by-site with the bacterial binding tests. The percentage of cells with bound FITC-labeled bacteria is indicated in each condition in the presence of mannan at 20 µg/ml. C) Internalization of gonococcal strains and E.coli DH5α into iMDDCs. Immature MDDCs were allowed to adhere onto coverslips pre-coated with 0.2% gelatin. These cells were pulsed with gonococcal strains or E.coli DH5α (MOI = 100) prelabeled with Texas red-X-succinimidyl ester at 37 o C for 1 h. Extracellular bacteria were then labeled with the polyclonal anti-gonococcal serum, followed by a staining with a BODIPY-FL-conjugated secondary Ab. Immature MDDCs were then permeabilized with 0.4% Triton X-100 and stained with Phalloidin-FITC. Intracellular (red) versus extracellular (yellow) bacteria with MDDCs (green) were then distinguished by visualization with a Leica DM-IRBE inverted fluorescence microscope. The magnification for all conditions is 40.

    Journal: PLoS ONE

    Article Title: Association of Neisseria gonorrhoeae OpaCEA with Dendritic Cells Suppresses Their Ability to Elicit an HIV-1-Specific T Cell Memory Response

    doi: 10.1371/journal.pone.0056705

    Figure Lengend Snippet: Gonococcal binding and internalization to iMDDCs. A ) Immature MDDCs were incubated with FITC-labeled gonococcal strains N302, N303, N309, and N496, or E.coli DH5α bacteria at an MOI of 10 for 30 min at RT. Bacterial binding was determined by measuring the percentage of cells that bound FITC-labeled bacteria using flow cytometric analysis. The percentage of cells with bound FITC-labeled bacteria is indicated in each condition. B) Bacterial binding to iMDDCs is not mediated by DC-SIGN on the cell surface. Mannan blocking assays were carried out site-by-site with the bacterial binding tests. The percentage of cells with bound FITC-labeled bacteria is indicated in each condition in the presence of mannan at 20 µg/ml. C) Internalization of gonococcal strains and E.coli DH5α into iMDDCs. Immature MDDCs were allowed to adhere onto coverslips pre-coated with 0.2% gelatin. These cells were pulsed with gonococcal strains or E.coli DH5α (MOI = 100) prelabeled with Texas red-X-succinimidyl ester at 37 o C for 1 h. Extracellular bacteria were then labeled with the polyclonal anti-gonococcal serum, followed by a staining with a BODIPY-FL-conjugated secondary Ab. Immature MDDCs were then permeabilized with 0.4% Triton X-100 and stained with Phalloidin-FITC. Intracellular (red) versus extracellular (yellow) bacteria with MDDCs (green) were then distinguished by visualization with a Leica DM-IRBE inverted fluorescence microscope. The magnification for all conditions is 40.

    Article Snippet: Intracellular (red) versus extracellular (yellow) bacteria with iMDDCs (green) were then distinguished by visualization with a Leica DM-IRBE inverted fluorescence microscope (Leica Microsystems, Toronto, Ontario).

    Techniques: Binding Assay, Incubation, Labeling, Flow Cytometry, Blocking Assay, Staining, Fluorescence, Microscopy