Structured Review

Nikon fluorescence intensity
Fluorescence Intensity, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence intensity/product/Nikon
Average 99 stars, based on 364 article reviews
Price from $9.99 to $1999.99
fluorescence intensity - by Bioz Stars, 2020-04
99/100 stars

Images

Related Articles

Transduction:

Article Title: Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice
Article Snippet: BMCs were transduced with either control or IL-10 virus and were then differentiated into BMDMs as described above. .. Fluorescence intensity was photographed using Nikon C1 Confocal System (Nikon, Tokyo, Japan) and quantified using ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA).

Centrifugation:

Article Title: Identification of a Quality Marker of Vinegar-Processed Curcuma Zedoaria on Oxidative Liver Injury
Article Snippet: Briefly, 1 mL of the algal suspension was collected after centrifugation (9000 g, 5 min) and then washed three times with phosphate buffered saline (PBS). .. The fluorescence intensity of DCF was measured using a fluorescence spectrophotometer (ECLIPSE Ts2R-FL, Nikon, Japan) with an excitation wavelength of 488 nm and an emission wavelength of 510 nm.

Cytometry:

Article Title: MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats By Upregulating Mitochondrial Translation
Article Snippet: Fluorescence intensity was measured under a Nikon DXM1200 fluorescence microscope and images were analyzed with the Image-Pro software (Media Cybernetics). mt-ROS was measured with MitoSOX Red (Invitrogen) on live cells, as described. .. After incubation, cells were trypsinized and washed with ice-cold phosphate-buffered saline 3 times. mt-ROS was quantified by flow cytometry (BD Biosciences) with 510 nm excitation/580 nm emission filters.

Blocking Assay:

Article Title: Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation
Article Snippet: The harvested cells or 7-μm retinal tissue frozen sections from the animals were fixed in 4% paraformaldehyde and blocked in blocking buffer. .. The double-positive staining of GFAP and IL-17 on reactive astrocytes was quantified by measuring the merged fluorescence intensity in the retina using the NIS-Elements BR version 4.00.00 software (Nikon).

Adsorption:

Article Title: A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces
Article Snippet: Fluorescence Measurements To confirm these results on more realistic surfaces and, simultaneously, confirm the non-fouling properties with a smaller protein size, albumin (66 kDa) adsorption on bare PET, LP- and LP-PEG-coated PET was studied using fluorescence microscopy. .. Fluorescence intensity (fluorescence excitation and emission of 596 and 615 nm, resp.), which directly correlated with the amount of albumin adsorbed on the surface [ ], was measured using the NIS-Elements AR (version 3.0) Nikon software, and the background was subtracted for each sample.

Incubation:

Article Title: Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation
Article Snippet: The cells or tissue sections were subsequently incubated with 2-(4- amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI; Roche, Basel, Switzerland) for the cell nuclei staining before the slides were mounted. .. The double-positive staining of GFAP and IL-17 on reactive astrocytes was quantified by measuring the merged fluorescence intensity in the retina using the NIS-Elements BR version 4.00.00 software (Nikon).

Article Title: Angiotensin II slow-pressor hypertension enhances NMDA currents and NOX2-dependent superoxide production in hypothalamic paraventricular neurons
Article Snippet: All isolated cells were incubated with DHE (1 μmol/l) for 30 min. .. The fluorescence intensity was measured using a Nikon diaphot 300 inverted microscope (Nikon, Japan) equipped with a charge-coupled device (CCD) camera (Princeton Instruments, Trenton, NJ) and HE bromide filter ( ).

Article Title: Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice
Article Snippet: BMDMs seeded on chamber slide (Nalge Nunc International, Rochester, NY, USA) were incubated with 10 μg/ml Dil-AcLDL in medium for 2 h at 37°C. .. Fluorescence intensity was photographed using Nikon C1 Confocal System (Nikon, Tokyo, Japan) and quantified using ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA).

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: To confirm the specificity of anti-integrin αV β3 antibody LM609, fixed cells were incubated with anti-integrin αV β3 antibody that was pretreated with recombinant human integrin αV β3 (R & D Systems, Wiesbaden-Nordenstadt, Germany). .. The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software.

Article Title: MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats By Upregulating Mitochondrial Translation
Article Snippet: Fluorescence intensity was measured under a Nikon DXM1200 fluorescence microscope and images were analyzed with the Image-Pro software (Media Cybernetics). mt-ROS was measured with MitoSOX Red (Invitrogen) on live cells, as described. .. MitoSOX Red (diluted to a final concentration of 5 µmol/L) was added to the media and incubated for 30 minutes at 37°C in the dark.

Article Title: Identification of a Quality Marker of Vinegar-Processed Curcuma Zedoaria on Oxidative Liver Injury
Article Snippet: The algal cells were incubated with DCFH-DA (10 μM) in the dark at 25 °C for 30 min. .. The fluorescence intensity of DCF was measured using a fluorescence spectrophotometer (ECLIPSE Ts2R-FL, Nikon, Japan) with an excitation wavelength of 488 nm and an emission wavelength of 510 nm.

Mass Spectrometry:

Article Title: Angiotensin II slow-pressor hypertension enhances NMDA currents and NOX2-dependent superoxide production in hypothalamic paraventricular neurons
Article Snippet: The fluorescence intensity was measured using a Nikon diaphot 300 inverted microscope (Nikon, Japan) equipped with a charge-coupled device (CCD) camera (Princeton Instruments, Trenton, NJ) and HE bromide filter ( ). .. Time-resolved fluorescence was measured every 30 s with an exposure time of 100 ms using image analysis software (IPLab; Scanalytics, Fairfax, VA).

Modification:

Article Title: Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice
Article Snippet: To determine the receptor-specific binding and uptake of modified LDL, fluorescence-labeled acetylated LDL (Dil-AcLDL) was used as described previously . .. Fluorescence intensity was photographed using Nikon C1 Confocal System (Nikon, Tokyo, Japan) and quantified using ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA).

Western Blot:

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: Paragraph title: Immunofluorescence and Western blot analysis. ... The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software.

MANN-WHITNEY:

Article Title: Astrocytes, but not microglia, are activated in oxaliplatin and bortezomib-induced peripheral neuropathy in the rat
Article Snippet: A region containing only background signal was selected within the slice, and the corresponding level of fluorescence intensity was analyzed using NIS Elements software (Nikon, USA). .. Significance was determined via Mann-Whitney test (α = 0.05, 0.025, 0.01).

Immunohistochemistry:

Article Title: Astrocytes, but not microglia, are activated in oxaliplatin and bortezomib-induced peripheral neuropathy in the rat
Article Snippet: Paragraph title: Quantification of immunohistochemistry ... A region containing only background signal was selected within the slice, and the corresponding level of fluorescence intensity was analyzed using NIS Elements software (Nikon, USA).

Imaging:

Article Title: Tissue Factor-Dependent Coagulation Is Preferentially Up-Regulated within Arterial Branching Areas in a Baboon Model of Escherichia coli Sepsis
Article Snippet: Specimens were examined by epifluorescence confocal imaging using a Nikon C1 confocal laser-scanning unit equipped with a three-laser launcher (488, 543, and 633 nm emission lines) installed on an Eclipse TE200-U inverted microscope (Nikon, Melville, NY). .. The measurement of fluorescence intensity in z -stacks was done using the EZ-C1 software (Nikon).

Article Title: Astrocytes, but not microglia, are activated in oxaliplatin and bortezomib-induced peripheral neuropathy in the rat
Article Snippet: The filter was then changed to fluorescence imaging, maintaining the location of the region of interest. .. A region containing only background signal was selected within the slice, and the corresponding level of fluorescence intensity was analyzed using NIS Elements software (Nikon, USA).

Inverted Microscopy:

Article Title: Angiotensin II slow-pressor hypertension enhances NMDA currents and NOX2-dependent superoxide production in hypothalamic paraventricular neurons
Article Snippet: .. The fluorescence intensity was measured using a Nikon diaphot 300 inverted microscope (Nikon, Japan) equipped with a charge-coupled device (CCD) camera (Princeton Instruments, Trenton, NJ) and HE bromide filter ( ). .. Time-resolved fluorescence was measured every 30 s with an exposure time of 100 ms using image analysis software (IPLab; Scanalytics, Fairfax, VA).

Article Title: Tissue Factor-Dependent Coagulation Is Preferentially Up-Regulated within Arterial Branching Areas in a Baboon Model of Escherichia coli Sepsis
Article Snippet: Specimens were examined by epifluorescence confocal imaging using a Nikon C1 confocal laser-scanning unit equipped with a three-laser launcher (488, 543, and 633 nm emission lines) installed on an Eclipse TE200-U inverted microscope (Nikon, Melville, NY). .. The measurement of fluorescence intensity in z -stacks was done using the EZ-C1 software (Nikon).

Binding Assay:

Article Title: Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice
Article Snippet: Paragraph title: Binding and uptake of AcLDL ... Fluorescence intensity was photographed using Nikon C1 Confocal System (Nikon, Tokyo, Japan) and quantified using ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA).

Immunofluorescence:

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: Paragraph title: Immunofluorescence and Western blot analysis. ... The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software.

In Vivo:

Article Title: The aged niche disrupts muscle stem cell quiescence
Article Snippet: Paragraph title: IN-VIVO CELL DIVISION ANALYSIS ... Fluorescence intensity of BrdU signal obtained by alexa-conjugated 546 secondary antibodies was converted to grey scale and quantified using Nikon Eclipse software.

Fluorescence:

Article Title: Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation
Article Snippet: .. The double-positive staining of GFAP and IL-17 on reactive astrocytes was quantified by measuring the merged fluorescence intensity in the retina using the NIS-Elements BR version 4.00.00 software (Nikon). .. Two-μm sections of the eyeball from the EAU, treated and control animals or the primary cultured neural cells were fixed in 4% paraformaldehyde and blocked in blocking buffer.

Article Title: Detection of Calcium Transients in Embryonic Stem Cells and Their Differentiated Progeny
Article Snippet: .. The fluorescence intensity of undifferentiated ES cell colonies or differentiated neural cells, as well as the background fluorescence, was acquired by using NIS Elements software (version 3.0, Nikon). .. Relative fluorescence intensity was calculated by comparing the mean fluorescence intensity of cells to the background fluorescence within each field.

Article Title: Angiotensin II slow-pressor hypertension enhances NMDA currents and NOX2-dependent superoxide production in hypothalamic paraventricular neurons
Article Snippet: .. The fluorescence intensity was measured using a Nikon diaphot 300 inverted microscope (Nikon, Japan) equipped with a charge-coupled device (CCD) camera (Princeton Instruments, Trenton, NJ) and HE bromide filter ( ). .. Time-resolved fluorescence was measured every 30 s with an exposure time of 100 ms using image analysis software (IPLab; Scanalytics, Fairfax, VA).

Article Title: Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice
Article Snippet: .. Fluorescence intensity was photographed using Nikon C1 Confocal System (Nikon, Tokyo, Japan) and quantified using ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA). .. For detecting lipid accumulation in lesions, Oil Red-O staining and BODIPY staining techniques were employed as described previously .

Article Title: The aged niche disrupts muscle stem cell quiescence
Article Snippet: .. Fluorescence intensity of BrdU signal obtained by alexa-conjugated 546 secondary antibodies was converted to grey scale and quantified using Nikon Eclipse software. ..

Article Title: A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces
Article Snippet: .. Fluorescence intensity (fluorescence excitation and emission of 596 and 615 nm, resp.), which directly correlated with the amount of albumin adsorbed on the surface [ ], was measured using the NIS-Elements AR (version 3.0) Nikon software, and the background was subtracted for each sample. .. Intensity for fluorescence measurements is given as counts per second (cps).

Article Title: Astrocytes, but not microglia, are activated in oxaliplatin and bortezomib-induced peripheral neuropathy in the rat
Article Snippet: .. A region containing only background signal was selected within the slice, and the corresponding level of fluorescence intensity was analyzed using NIS Elements software (Nikon, USA). ..

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: .. The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software. .. Mean intensities of glomerular ZO-1 staining in renal biopsy specimens of hantavirus patients and controls were statistically compared by using a Student t test.

Article Title: Regulation of the human Na+-dependent glucose cotransporter hSGLT2
Article Snippet: .. The same day cells were plated on 12-mm poly- l -lysine-coated glass coverslips and selected on the basis of fluorescence intensity using a Nikon diaphot epifluorescence microscope (Nikon, Tokyo, Japan). ..

Article Title: MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats By Upregulating Mitochondrial Translation
Article Snippet: .. Fluorescence intensity was measured under a Nikon DXM1200 fluorescence microscope and images were analyzed with the Image-Pro software (Media Cybernetics). mt-ROS was measured with MitoSOX Red (Invitrogen) on live cells, as described. ..

Article Title: Identification of a Quality Marker of Vinegar-Processed Curcuma Zedoaria on Oxidative Liver Injury
Article Snippet: .. The fluorescence intensity of DCF was measured using a fluorescence spectrophotometer (ECLIPSE Ts2R-FL, Nikon, Japan) with an excitation wavelength of 488 nm and an emission wavelength of 510 nm. .. Tube Formation Assay Formation of tube networks was assessed as described before (Borradaile and Pickering, 2009; Das, 2018).

Isolation:

Article Title: Angiotensin II slow-pressor hypertension enhances NMDA currents and NOX2-dependent superoxide production in hypothalamic paraventricular neurons
Article Snippet: All isolated cells were incubated with DHE (1 μmol/l) for 30 min. .. The fluorescence intensity was measured using a Nikon diaphot 300 inverted microscope (Nikon, Japan) equipped with a charge-coupled device (CCD) camera (Princeton Instruments, Trenton, NJ) and HE bromide filter ( ).

Flow Cytometry:

Article Title: MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats By Upregulating Mitochondrial Translation
Article Snippet: Fluorescence intensity was measured under a Nikon DXM1200 fluorescence microscope and images were analyzed with the Image-Pro software (Media Cybernetics). mt-ROS was measured with MitoSOX Red (Invitrogen) on live cells, as described. .. After incubation, cells were trypsinized and washed with ice-cold phosphate-buffered saline 3 times. mt-ROS was quantified by flow cytometry (BD Biosciences) with 510 nm excitation/580 nm emission filters.

Microscopy:

Article Title: Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation
Article Snippet: The fluorescent images were taken by a fluorescence microscope (Nikon 80i) with a cold CCD camera (Nikon DS-Ri1) by the NIS-Elements F version 3.0 software. .. The double-positive staining of GFAP and IL-17 on reactive astrocytes was quantified by measuring the merged fluorescence intensity in the retina using the NIS-Elements BR version 4.00.00 software (Nikon).

Article Title: A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces
Article Snippet: Surfaces were then washed thoroughly with PBS to remove unbound protein, dried with a stream of nitrogen and examined under a fluorescence microscope (Nikon Eclipse E600 mounted with a Photometrics CoolSnap HQ2 CCD camera) at 10x magnification. .. Fluorescence intensity (fluorescence excitation and emission of 596 and 615 nm, resp.), which directly correlated with the amount of albumin adsorbed on the surface [ ], was measured using the NIS-Elements AR (version 3.0) Nikon software, and the background was subtracted for each sample.

Article Title: Astrocytes, but not microglia, are activated in oxaliplatin and bortezomib-induced peripheral neuropathy in the rat
Article Snippet: Slices were viewed and images captured using light and fluorescent illumination at 10X using a Nikon Eclipse E600 microscope. .. A region containing only background signal was selected within the slice, and the corresponding level of fluorescence intensity was analyzed using NIS Elements software (Nikon, USA).

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: Images were taken using a Nikon DXM1200C camera attached to a Nikon Eclipse 80i upright microscope (Nikon, Düsseldorf, Germany). .. The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software.

Article Title: Regulation of the human Na+-dependent glucose cotransporter hSGLT2
Article Snippet: .. The same day cells were plated on 12-mm poly- l -lysine-coated glass coverslips and selected on the basis of fluorescence intensity using a Nikon diaphot epifluorescence microscope (Nikon, Tokyo, Japan). ..

Article Title: MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats By Upregulating Mitochondrial Translation
Article Snippet: .. Fluorescence intensity was measured under a Nikon DXM1200 fluorescence microscope and images were analyzed with the Image-Pro software (Media Cybernetics). mt-ROS was measured with MitoSOX Red (Invitrogen) on live cells, as described. ..

Mouse Assay:

Article Title: The aged niche disrupts muscle stem cell quiescence
Article Snippet: For label retention studies, Spry1flx/flx mice were given 3 daily intraperitoneal (IP) injections of Tmx (diluted in corn oil (Sigma)) following BrdU loading and placed on regular drinking water to chase label. .. Fluorescence intensity of BrdU signal obtained by alexa-conjugated 546 secondary antibodies was converted to grey scale and quantified using Nikon Eclipse software.

Positron Emission Tomography:

Article Title: A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces
Article Snippet: Fluorescence Measurements To confirm these results on more realistic surfaces and, simultaneously, confirm the non-fouling properties with a smaller protein size, albumin (66 kDa) adsorption on bare PET, LP- and LP-PEG-coated PET was studied using fluorescence microscopy. .. Fluorescence intensity (fluorescence excitation and emission of 596 and 615 nm, resp.), which directly correlated with the amount of albumin adsorbed on the surface [ ], was measured using the NIS-Elements AR (version 3.0) Nikon software, and the background was subtracted for each sample.

Immunostaining:

Article Title: The aged niche disrupts muscle stem cell quiescence
Article Snippet: For cell proliferation and label retention studies satellite cells (SCs) were sorted, immediately plated and processed for immunostaining with Pax7 and BrdU antibodies after sodium citrate antigen retrieval . .. Fluorescence intensity of BrdU signal obtained by alexa-conjugated 546 secondary antibodies was converted to grey scale and quantified using Nikon Eclipse software.

Polyacrylamide Gel Electrophoresis:

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software. .. For Western blot analysis, cells were lysed and, after being boiled in sodium dodecyl sulfate (SDS) sample buffer and separated by SDS-10% PAGE, transferred to a nitrocellulose membrane.

Software:

Article Title: Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation
Article Snippet: .. The double-positive staining of GFAP and IL-17 on reactive astrocytes was quantified by measuring the merged fluorescence intensity in the retina using the NIS-Elements BR version 4.00.00 software (Nikon). .. Two-μm sections of the eyeball from the EAU, treated and control animals or the primary cultured neural cells were fixed in 4% paraformaldehyde and blocked in blocking buffer.

Article Title: Detection of Calcium Transients in Embryonic Stem Cells and Their Differentiated Progeny
Article Snippet: .. The fluorescence intensity of undifferentiated ES cell colonies or differentiated neural cells, as well as the background fluorescence, was acquired by using NIS Elements software (version 3.0, Nikon). .. Relative fluorescence intensity was calculated by comparing the mean fluorescence intensity of cells to the background fluorescence within each field.

Article Title: Angiotensin II slow-pressor hypertension enhances NMDA currents and NOX2-dependent superoxide production in hypothalamic paraventricular neurons
Article Snippet: The fluorescence intensity was measured using a Nikon diaphot 300 inverted microscope (Nikon, Japan) equipped with a charge-coupled device (CCD) camera (Princeton Instruments, Trenton, NJ) and HE bromide filter ( ). .. Time-resolved fluorescence was measured every 30 s with an exposure time of 100 ms using image analysis software (IPLab; Scanalytics, Fairfax, VA).

Article Title: Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice
Article Snippet: .. Fluorescence intensity was photographed using Nikon C1 Confocal System (Nikon, Tokyo, Japan) and quantified using ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA). .. For detecting lipid accumulation in lesions, Oil Red-O staining and BODIPY staining techniques were employed as described previously .

Article Title: The aged niche disrupts muscle stem cell quiescence
Article Snippet: .. Fluorescence intensity of BrdU signal obtained by alexa-conjugated 546 secondary antibodies was converted to grey scale and quantified using Nikon Eclipse software. ..

Article Title: A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces
Article Snippet: .. Fluorescence intensity (fluorescence excitation and emission of 596 and 615 nm, resp.), which directly correlated with the amount of albumin adsorbed on the surface [ ], was measured using the NIS-Elements AR (version 3.0) Nikon software, and the background was subtracted for each sample. .. Intensity for fluorescence measurements is given as counts per second (cps).

Article Title: Tissue Factor-Dependent Coagulation Is Preferentially Up-Regulated within Arterial Branching Areas in a Baboon Model of Escherichia coli Sepsis
Article Snippet: .. The measurement of fluorescence intensity in z -stacks was done using the EZ-C1 software (Nikon). .. Briefly, single-channel grayscale images (12 bit, 4095 gray levels/pixel) were collected at 5-μm z -steps and the average fluorescence intensity of each image was integrated.

Article Title: Astrocytes, but not microglia, are activated in oxaliplatin and bortezomib-induced peripheral neuropathy in the rat
Article Snippet: .. A region containing only background signal was selected within the slice, and the corresponding level of fluorescence intensity was analyzed using NIS Elements software (Nikon, USA). ..

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: .. The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software. .. Mean intensities of glomerular ZO-1 staining in renal biopsy specimens of hantavirus patients and controls were statistically compared by using a Student t test.

Article Title: MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats By Upregulating Mitochondrial Translation
Article Snippet: .. Fluorescence intensity was measured under a Nikon DXM1200 fluorescence microscope and images were analyzed with the Image-Pro software (Media Cybernetics). mt-ROS was measured with MitoSOX Red (Invitrogen) on live cells, as described. ..

ROS Assay:

Article Title: Identification of a Quality Marker of Vinegar-Processed Curcuma Zedoaria on Oxidative Liver Injury
Article Snippet: Paragraph title: 4.8. Reactive Oxygen Species (ROS) Assay ... The fluorescence intensity of DCF was measured using a fluorescence spectrophotometer (ECLIPSE Ts2R-FL, Nikon, Japan) with an excitation wavelength of 488 nm and an emission wavelength of 510 nm.

Recombinant:

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: Recombinant protein was added to a final concentration of 0.04 μg/μl to integrin αV β3 antibody LM609 (final concentration, 0.01 μg/μl). .. The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software.

Patch Clamp:

Article Title: Regulation of the human Na+-dependent glucose cotransporter hSGLT2
Article Snippet: Paragraph title: Whole cell patch-clamp recording. ... The same day cells were plated on 12-mm poly- l -lysine-coated glass coverslips and selected on the basis of fluorescence intensity using a Nikon diaphot epifluorescence microscope (Nikon, Tokyo, Japan).

Spectrophotometry:

Article Title: Identification of a Quality Marker of Vinegar-Processed Curcuma Zedoaria on Oxidative Liver Injury
Article Snippet: .. The fluorescence intensity of DCF was measured using a fluorescence spectrophotometer (ECLIPSE Ts2R-FL, Nikon, Japan) with an excitation wavelength of 488 nm and an emission wavelength of 510 nm. .. Tube Formation Assay Formation of tube networks was assessed as described before (Borradaile and Pickering, 2009; Das, 2018).

Produced:

Article Title: Identification of a Quality Marker of Vinegar-Processed Curcuma Zedoaria on Oxidative Liver Injury
Article Snippet: DCFH-DA entered the algal cells, reacted with ROS, and then produced the highly fluorescent compound dichlorofluorescein (DCF). .. The fluorescence intensity of DCF was measured using a fluorescence spectrophotometer (ECLIPSE Ts2R-FL, Nikon, Japan) with an excitation wavelength of 488 nm and an emission wavelength of 510 nm.

Concentration Assay:

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: Recombinant protein was added to a final concentration of 0.04 μg/μl to integrin αV β3 antibody LM609 (final concentration, 0.01 μg/μl). .. The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software.

Article Title: MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats By Upregulating Mitochondrial Translation
Article Snippet: Fluorescence intensity was measured under a Nikon DXM1200 fluorescence microscope and images were analyzed with the Image-Pro software (Media Cybernetics). mt-ROS was measured with MitoSOX Red (Invitrogen) on live cells, as described. .. MitoSOX Red (diluted to a final concentration of 5 µmol/L) was added to the media and incubated for 30 minutes at 37°C in the dark.

Staining:

Article Title: Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation
Article Snippet: .. The double-positive staining of GFAP and IL-17 on reactive astrocytes was quantified by measuring the merged fluorescence intensity in the retina using the NIS-Elements BR version 4.00.00 software (Nikon). .. Two-μm sections of the eyeball from the EAU, treated and control animals or the primary cultured neural cells were fixed in 4% paraformaldehyde and blocked in blocking buffer.

Article Title: Tissue Factor-Dependent Coagulation Is Preferentially Up-Regulated within Arterial Branching Areas in a Baboon Model of Escherichia coli Sepsis
Article Snippet: Paragraph title: Whole Mount en Face Staining ... The measurement of fluorescence intensity in z -stacks was done using the EZ-C1 software (Nikon).

Article Title: Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts ▿
Article Snippet: The fluorescence intensity of the selected areas in 32 glomeruli of seven patients and of 18 glomeruli of two uninfected control kidneys was measured with Nikon NIS Elements Software. .. Mean intensities of glomerular ZO-1 staining in renal biopsy specimens of hantavirus patients and controls were statistically compared by using a Student t test.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Nikon gfap fluorescence intensity
    The <t>GFAP</t> promoter is astrocyte-specific. (A and B) Two weeks after systemic delivery of either <t>rAAV1/2-GFAP-GFP</t> or rAAV1/2-HBA-GFP, transgene-positive (GFP, green) cells are visible in the FUS-targeted region. Aβ plaque is shown in blue, and GFAP expression in red. (C) At higher magnification in the cortex, the morphology of GFP-positive cells in the rAAV1/2-GFAP-GFP group is consistent and colocalizes with GFAP-positive cells (colocalization, yellow). (D) In the cortex after delivery of rAAV1/2-HBA-GFP, a variety of cell morphologies are visible and are not always colocalized with GFAP. (E and F) In the hippocampus, the same respective trends are seen of GFP-positive cell morphology after delivery of rAAV1/2-GFAP-GFP or rAAV1/2-HBA-GFP. (G) As seen in an orthogonal projection, consistent colocalization between GFP-positive cells and GFAP is verified in the rAAV1/2-GFAP-GFP group. (H) A consistent colocalization between GFP and GFAP is not seen in the orthogonal projection of rAAV1/2-HBA-GFP cells. (I) Quantification of GFP-positive (GFP+) cells are categorized as GFAP+ (astrocyte), or GFAP- (undefined). The percentage of GFP-positive cells that are also GFAP-positive after rAAV1/2-GFAP-GFP delivery indicate almost exclusive transgene expression in astrocytes. (J) The areas containing GFP-positive cells in the cortex and hippocampus after FUS application were not significantly different in size (µm 2 ) between the rAAV1/2-GFAP-GFP and rAAV1/2-HBA-GFP groups (p=0.91). (K) The amount of BBB opening by FUS application can be estimated by the MRI enhancement from background of each focal spot, which was not significantly different between the two rAAV groups (p=0.87). (L) The number of Aβ plaques in the cortical and hippocampal regions containing GFP-positive cells was also not significantly different between rAAV groups (p=0.69). Data is represented as mean ±SEM and (I-L) n=4 animals per group. (K) For MRI enhancement n=12 focal spots from 4 animals, per group. Scale bars: (A and B) 1 mm; (c-f) 100 µm; (G and H) 20 µm.
    Gfap Fluorescence Intensity, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfap fluorescence intensity/product/Nikon
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfap fluorescence intensity - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    86
    Nikon dynein fluorescence intensity quantification
    Similar amounts of KT motors <t>(CENP-E</t> and <t>dynein)</t> accumulate at middle and peripheral KTs. (A) Examples of metaphase PtK1 cells immunostained for CENP-E (top, first column) or dynein (bottom, first column) and ACA (second column). The chromosomes were stained with DAPI. In the merged images, DAPI is shown in blue, ACA in red, and the motors in green. Bar, 5 µm. (B and C) Quantification of CENP-E (B) and dynein (C) at the KTs of middle versus peripheral KTs ( n = 46 cells) Error bars indicate mean ± SEM.
    Dynein Fluorescence Intensity Quantification, supplied by Nikon, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynein fluorescence intensity quantification/product/Nikon
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dynein fluorescence intensity quantification - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    86
    Nikon tmre fluorescence intensity
    Ketamine decreases mitochondrial membrane potential (ΔΨ m ) and increases cytochrome c release form mitochondria into cytosol A, ΔΨ m assay. Cells were loaded with mitochondrial probe <t>TMRE</t> and imaged with the <t>confocol</t> microscope. The fluorescent intensity of TMRE represents ΔΨ m . The results show that 100 μM ketamine treatment for 24 h decreased ΔΨ m (*P
    Tmre Fluorescence Intensity, supplied by Nikon, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tmre fluorescence intensity/product/Nikon
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tmre fluorescence intensity - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    86
    Nikon fluorescence intensity measurements comparing flotillin labeling
    Flotillins contribute to endocytosis in BeWo cells. (A) Flotillins co-localize with a fluorescent marker of fluid phase-pinocytosis in endocytosis assays. Mononuclear BeWo cells were incubated continuously in the presence of 1 mg/ml of lucifer yellow (LY-CH, green) for 15 min prior to fixation. Following permeabilization, the cells were immunolabeled with anti-FLOT2 antibodies (red) and imaged using confocal microscopy. In the representative micrograph taken from a single optical section, areas of co-localization between the LY-CH cargo and FLOT2 (yellow in the merged images, indicated by arrows) are observed. Similar results were obtained when the cells were labeled using antibodies generated against FLOT1 (not shown). Scale bar = 10 μm. (B) Immunoblots of BeWo cell lines stably transuced with shRNA targeting FLOT1, FLOT2, or both. Control cells were tranduced with Non-Target Control Transduction Particles (LV CTRL) as described in “Materials and methods”. (C) <t>Flotillin</t> knockdown decreases uptake of fluorescently-labeled cholera toxin B subunit (CTB-594). BeWo cells varying in flotillin expression levels were incubated with 20 μg/ml CTB-594 at 4°C for 30 min, then warmed to 37°C and endocytosis was allowed to proceed in media without labeled ligand for 15 min. On average, cells deficient in flotillin expression exhibited decreased CTB-549 based on average fluorescence intensity measurements. Data are mean ± SD from two separate experiments. Different letters indicate significant differences at P
    Fluorescence Intensity Measurements Comparing Flotillin Labeling, supplied by Nikon, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence intensity measurements comparing flotillin labeling/product/Nikon
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescence intensity measurements comparing flotillin labeling - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    The GFAP promoter is astrocyte-specific. (A and B) Two weeks after systemic delivery of either rAAV1/2-GFAP-GFP or rAAV1/2-HBA-GFP, transgene-positive (GFP, green) cells are visible in the FUS-targeted region. Aβ plaque is shown in blue, and GFAP expression in red. (C) At higher magnification in the cortex, the morphology of GFP-positive cells in the rAAV1/2-GFAP-GFP group is consistent and colocalizes with GFAP-positive cells (colocalization, yellow). (D) In the cortex after delivery of rAAV1/2-HBA-GFP, a variety of cell morphologies are visible and are not always colocalized with GFAP. (E and F) In the hippocampus, the same respective trends are seen of GFP-positive cell morphology after delivery of rAAV1/2-GFAP-GFP or rAAV1/2-HBA-GFP. (G) As seen in an orthogonal projection, consistent colocalization between GFP-positive cells and GFAP is verified in the rAAV1/2-GFAP-GFP group. (H) A consistent colocalization between GFP and GFAP is not seen in the orthogonal projection of rAAV1/2-HBA-GFP cells. (I) Quantification of GFP-positive (GFP+) cells are categorized as GFAP+ (astrocyte), or GFAP- (undefined). The percentage of GFP-positive cells that are also GFAP-positive after rAAV1/2-GFAP-GFP delivery indicate almost exclusive transgene expression in astrocytes. (J) The areas containing GFP-positive cells in the cortex and hippocampus after FUS application were not significantly different in size (µm 2 ) between the rAAV1/2-GFAP-GFP and rAAV1/2-HBA-GFP groups (p=0.91). (K) The amount of BBB opening by FUS application can be estimated by the MRI enhancement from background of each focal spot, which was not significantly different between the two rAAV groups (p=0.87). (L) The number of Aβ plaques in the cortical and hippocampal regions containing GFP-positive cells was also not significantly different between rAAV groups (p=0.69). Data is represented as mean ±SEM and (I-L) n=4 animals per group. (K) For MRI enhancement n=12 focal spots from 4 animals, per group. Scale bars: (A and B) 1 mm; (c-f) 100 µm; (G and H) 20 µm.

    Journal: Theranostics

    Article Title: Strategy to enhance transgene expression in proximity of amyloid plaques in a mouse model of Alzheimer's disease

    doi: 10.7150/thno.36718

    Figure Lengend Snippet: The GFAP promoter is astrocyte-specific. (A and B) Two weeks after systemic delivery of either rAAV1/2-GFAP-GFP or rAAV1/2-HBA-GFP, transgene-positive (GFP, green) cells are visible in the FUS-targeted region. Aβ plaque is shown in blue, and GFAP expression in red. (C) At higher magnification in the cortex, the morphology of GFP-positive cells in the rAAV1/2-GFAP-GFP group is consistent and colocalizes with GFAP-positive cells (colocalization, yellow). (D) In the cortex after delivery of rAAV1/2-HBA-GFP, a variety of cell morphologies are visible and are not always colocalized with GFAP. (E and F) In the hippocampus, the same respective trends are seen of GFP-positive cell morphology after delivery of rAAV1/2-GFAP-GFP or rAAV1/2-HBA-GFP. (G) As seen in an orthogonal projection, consistent colocalization between GFP-positive cells and GFAP is verified in the rAAV1/2-GFAP-GFP group. (H) A consistent colocalization between GFP and GFAP is not seen in the orthogonal projection of rAAV1/2-HBA-GFP cells. (I) Quantification of GFP-positive (GFP+) cells are categorized as GFAP+ (astrocyte), or GFAP- (undefined). The percentage of GFP-positive cells that are also GFAP-positive after rAAV1/2-GFAP-GFP delivery indicate almost exclusive transgene expression in astrocytes. (J) The areas containing GFP-positive cells in the cortex and hippocampus after FUS application were not significantly different in size (µm 2 ) between the rAAV1/2-GFAP-GFP and rAAV1/2-HBA-GFP groups (p=0.91). (K) The amount of BBB opening by FUS application can be estimated by the MRI enhancement from background of each focal spot, which was not significantly different between the two rAAV groups (p=0.87). (L) The number of Aβ plaques in the cortical and hippocampal regions containing GFP-positive cells was also not significantly different between rAAV groups (p=0.69). Data is represented as mean ±SEM and (I-L) n=4 animals per group. (K) For MRI enhancement n=12 focal spots from 4 animals, per group. Scale bars: (A and B) 1 mm; (c-f) 100 µm; (G and H) 20 µm.

    Article Snippet: Fluorescence Quantification The GFP and GFAP fluorescence intensity per unit volume, as well as GFP expression volume and surface area of a GFP and GFAP-positive cell, either associated or unassociated with Aβ plaque, was compared using the 3D Measurement module of Nikon Elements software (Nikon Instruments).

    Techniques: Expressing, Magnetic Resonance Imaging

    GFAP promoter permits transgene expression in the liver. (A) Under control of the GFAP promoter, GFP expression (green) was not prevented in the liver, despite an absence of GFAP protein detection (red). Cell nuclei are shown in blue (DAPI). (B) Under control of the HBA promoter, GFP was also expressed in the liver. (C and D) Both promoters lead to expression in the kidney, (E and F) while only the rAAV1/2-HBA-GFP group showed GFP expression in the heart. (G) GFP expression was not seen in the quadriceps muscle of the rAAV1/2-GFAP-GFP group, (H) but was detected in the rAAV1/2-HBA-GFP group. (I) GFP expression was not detected in the spleen after delivery of rAAV1/2-GFAP-GFP, (J) but was detected in the rAAV1/2-HBA-GFP group. (K and L) No GFP expression was detected in the lung for either the GFAP or HBA promoter groups. (A-L) Scale bar, 50 µm.

    Journal: Theranostics

    Article Title: Strategy to enhance transgene expression in proximity of amyloid plaques in a mouse model of Alzheimer's disease

    doi: 10.7150/thno.36718

    Figure Lengend Snippet: GFAP promoter permits transgene expression in the liver. (A) Under control of the GFAP promoter, GFP expression (green) was not prevented in the liver, despite an absence of GFAP protein detection (red). Cell nuclei are shown in blue (DAPI). (B) Under control of the HBA promoter, GFP was also expressed in the liver. (C and D) Both promoters lead to expression in the kidney, (E and F) while only the rAAV1/2-HBA-GFP group showed GFP expression in the heart. (G) GFP expression was not seen in the quadriceps muscle of the rAAV1/2-GFAP-GFP group, (H) but was detected in the rAAV1/2-HBA-GFP group. (I) GFP expression was not detected in the spleen after delivery of rAAV1/2-GFAP-GFP, (J) but was detected in the rAAV1/2-HBA-GFP group. (K and L) No GFP expression was detected in the lung for either the GFAP or HBA promoter groups. (A-L) Scale bar, 50 µm.

    Article Snippet: Fluorescence Quantification The GFP and GFAP fluorescence intensity per unit volume, as well as GFP expression volume and surface area of a GFP and GFAP-positive cell, either associated or unassociated with Aβ plaque, was compared using the 3D Measurement module of Nikon Elements software (Nikon Instruments).

    Techniques: Expressing

    GFAP promoter-driven expression of GFP intensified in the vicinity of Aβ plaque. (A-D) rAAV1/2-GFAP-GFP expression in GFAP-positive cells (red) with processes overlapping in space with Aβ plaque (blue) show a distinct pattern of expression in both the cell body and processes compared to (E-H) rAAV1/2-GFAP-GFP expression in the absence of Aβ plaque and rAAV1/2-HBA-GFP expression both (I-L) associated and (M-P) unassociated with Aβ plaque. Scale bar, 20 µm.

    Journal: Theranostics

    Article Title: Strategy to enhance transgene expression in proximity of amyloid plaques in a mouse model of Alzheimer's disease

    doi: 10.7150/thno.36718

    Figure Lengend Snippet: GFAP promoter-driven expression of GFP intensified in the vicinity of Aβ plaque. (A-D) rAAV1/2-GFAP-GFP expression in GFAP-positive cells (red) with processes overlapping in space with Aβ plaque (blue) show a distinct pattern of expression in both the cell body and processes compared to (E-H) rAAV1/2-GFAP-GFP expression in the absence of Aβ plaque and rAAV1/2-HBA-GFP expression both (I-L) associated and (M-P) unassociated with Aβ plaque. Scale bar, 20 µm.

    Article Snippet: Fluorescence Quantification The GFP and GFAP fluorescence intensity per unit volume, as well as GFP expression volume and surface area of a GFP and GFAP-positive cell, either associated or unassociated with Aβ plaque, was compared using the 3D Measurement module of Nikon Elements software (Nikon Instruments).

    Techniques: Expressing

    GFAP promoter results in greater GFP fluorescence intensity, volume, and surface area of astrocytes near Aβ plaque. (A) Quantification of GFP fluorescence per unit volume is significantly increased in GFAP and GFP-positive cells near Aβ plaque in the rAAV1/2-GFAP-GFP group, compared to GFAP and GFP-positive cells unassociated with Aβ plaque (**p

    Journal: Theranostics

    Article Title: Strategy to enhance transgene expression in proximity of amyloid plaques in a mouse model of Alzheimer's disease

    doi: 10.7150/thno.36718

    Figure Lengend Snippet: GFAP promoter results in greater GFP fluorescence intensity, volume, and surface area of astrocytes near Aβ plaque. (A) Quantification of GFP fluorescence per unit volume is significantly increased in GFAP and GFP-positive cells near Aβ plaque in the rAAV1/2-GFAP-GFP group, compared to GFAP and GFP-positive cells unassociated with Aβ plaque (**p

    Article Snippet: Fluorescence Quantification The GFP and GFAP fluorescence intensity per unit volume, as well as GFP expression volume and surface area of a GFP and GFAP-positive cell, either associated or unassociated with Aβ plaque, was compared using the 3D Measurement module of Nikon Elements software (Nikon Instruments).

    Techniques: Fluorescence

    Similar amounts of KT motors (CENP-E and dynein) accumulate at middle and peripheral KTs. (A) Examples of metaphase PtK1 cells immunostained for CENP-E (top, first column) or dynein (bottom, first column) and ACA (second column). The chromosomes were stained with DAPI. In the merged images, DAPI is shown in blue, ACA in red, and the motors in green. Bar, 5 µm. (B and C) Quantification of CENP-E (B) and dynein (C) at the KTs of middle versus peripheral KTs ( n = 46 cells) Error bars indicate mean ± SEM.

    Journal: The Journal of Cell Biology

    Article Title: Dynamic bonds and polar ejection force distribution explain kinetochore oscillations in PtK1 cells

    doi: 10.1083/jcb.201301022

    Figure Lengend Snippet: Similar amounts of KT motors (CENP-E and dynein) accumulate at middle and peripheral KTs. (A) Examples of metaphase PtK1 cells immunostained for CENP-E (top, first column) or dynein (bottom, first column) and ACA (second column). The chromosomes were stained with DAPI. In the merged images, DAPI is shown in blue, ACA in red, and the motors in green. Bar, 5 µm. (B and C) Quantification of CENP-E (B) and dynein (C) at the KTs of middle versus peripheral KTs ( n = 46 cells) Error bars indicate mean ± SEM.

    Article Snippet: For CENP-E and dynein fluorescence intensity quantification, immunostained cells were imaged with an inverted microscope (Eclipse Ti; Nikon) with a Lumen 200PRO fluorescence illumination system (Prior Scientific).

    Techniques: Staining

    Ketamine decreases mitochondrial membrane potential (ΔΨ m ) and increases cytochrome c release form mitochondria into cytosol A, ΔΨ m assay. Cells were loaded with mitochondrial probe TMRE and imaged with the confocol microscope. The fluorescent intensity of TMRE represents ΔΨ m . The results show that 100 μM ketamine treatment for 24 h decreased ΔΨ m (*P

    Journal: Anesthesia and analgesia

    Article Title: Ketamine Enhances Human Neural Stem Cell Proliferation and Induces Neuronal Apoptosis Via Reactive Oxygen Species-Mediated Mitochondrial Pathway

    doi: 10.1213/ANE.0b013e3182860fc9

    Figure Lengend Snippet: Ketamine decreases mitochondrial membrane potential (ΔΨ m ) and increases cytochrome c release form mitochondria into cytosol A, ΔΨ m assay. Cells were loaded with mitochondrial probe TMRE and imaged with the confocol microscope. The fluorescent intensity of TMRE represents ΔΨ m . The results show that 100 μM ketamine treatment for 24 h decreased ΔΨ m (*P

    Article Snippet: TMRE fluorescence intensity representing ΔΨm was recorded under the confocol microscopy with a 60×1.4 numerical aperture oil immersion objective (Nikon).

    Techniques: Microscopy

    Flotillins contribute to endocytosis in BeWo cells. (A) Flotillins co-localize with a fluorescent marker of fluid phase-pinocytosis in endocytosis assays. Mononuclear BeWo cells were incubated continuously in the presence of 1 mg/ml of lucifer yellow (LY-CH, green) for 15 min prior to fixation. Following permeabilization, the cells were immunolabeled with anti-FLOT2 antibodies (red) and imaged using confocal microscopy. In the representative micrograph taken from a single optical section, areas of co-localization between the LY-CH cargo and FLOT2 (yellow in the merged images, indicated by arrows) are observed. Similar results were obtained when the cells were labeled using antibodies generated against FLOT1 (not shown). Scale bar = 10 μm. (B) Immunoblots of BeWo cell lines stably transuced with shRNA targeting FLOT1, FLOT2, or both. Control cells were tranduced with Non-Target Control Transduction Particles (LV CTRL) as described in “Materials and methods”. (C) Flotillin knockdown decreases uptake of fluorescently-labeled cholera toxin B subunit (CTB-594). BeWo cells varying in flotillin expression levels were incubated with 20 μg/ml CTB-594 at 4°C for 30 min, then warmed to 37°C and endocytosis was allowed to proceed in media without labeled ligand for 15 min. On average, cells deficient in flotillin expression exhibited decreased CTB-549 based on average fluorescence intensity measurements. Data are mean ± SD from two separate experiments. Different letters indicate significant differences at P

    Journal: Histochemistry and cell biology

    Article Title: Expression of flotillins in the human placenta: potential implications for placental transcytosis

    doi: 10.1007/s00418-012-1040-2

    Figure Lengend Snippet: Flotillins contribute to endocytosis in BeWo cells. (A) Flotillins co-localize with a fluorescent marker of fluid phase-pinocytosis in endocytosis assays. Mononuclear BeWo cells were incubated continuously in the presence of 1 mg/ml of lucifer yellow (LY-CH, green) for 15 min prior to fixation. Following permeabilization, the cells were immunolabeled with anti-FLOT2 antibodies (red) and imaged using confocal microscopy. In the representative micrograph taken from a single optical section, areas of co-localization between the LY-CH cargo and FLOT2 (yellow in the merged images, indicated by arrows) are observed. Similar results were obtained when the cells were labeled using antibodies generated against FLOT1 (not shown). Scale bar = 10 μm. (B) Immunoblots of BeWo cell lines stably transuced with shRNA targeting FLOT1, FLOT2, or both. Control cells were tranduced with Non-Target Control Transduction Particles (LV CTRL) as described in “Materials and methods”. (C) Flotillin knockdown decreases uptake of fluorescently-labeled cholera toxin B subunit (CTB-594). BeWo cells varying in flotillin expression levels were incubated with 20 μg/ml CTB-594 at 4°C for 30 min, then warmed to 37°C and endocytosis was allowed to proceed in media without labeled ligand for 15 min. On average, cells deficient in flotillin expression exhibited decreased CTB-549 based on average fluorescence intensity measurements. Data are mean ± SD from two separate experiments. Different letters indicate significant differences at P

    Article Snippet: Fluorescence intensity measurements comparing flotillin labeling in different regions of the villous tree were made using the intensity profile tool of the NIS-Elements AR software package (version 3.1, Nikon Instruments).

    Techniques: Marker, Incubation, Immunolabeling, Confocal Microscopy, Labeling, Generated, Western Blot, Stable Transfection, shRNA, Transduction, CtB Assay, Expressing, Fluorescence

    ) were resolved by gel electrophoresis, transferred to nitrocellulose membranes, and probed with antibodies directed against either FLOT1 or FLOT2. These fractions were: crude tissue homogenate (CTH), filtered crude tissue homogenate (FCTH), crude placental supernatant (CPS), crude placental pellet (CPP), Histodenz fractions (0–50%, 50–55%), and the pelleted plasma membrane (65% PPM). Note that neither FLOT1 nor FLOT2 was enriched in the PPM fraction. (B) Immunoblotting was used to confirm the expression of both flotillins in three additional term human placenta extracts prepared following lysis in buffer containing n -octyl β-D-glucopyranoside (see “Materials and methods”).

    Journal: Histochemistry and cell biology

    Article Title: Expression of flotillins in the human placenta: potential implications for placental transcytosis

    doi: 10.1007/s00418-012-1040-2

    Figure Lengend Snippet: ) were resolved by gel electrophoresis, transferred to nitrocellulose membranes, and probed with antibodies directed against either FLOT1 or FLOT2. These fractions were: crude tissue homogenate (CTH), filtered crude tissue homogenate (FCTH), crude placental supernatant (CPS), crude placental pellet (CPP), Histodenz fractions (0–50%, 50–55%), and the pelleted plasma membrane (65% PPM). Note that neither FLOT1 nor FLOT2 was enriched in the PPM fraction. (B) Immunoblotting was used to confirm the expression of both flotillins in three additional term human placenta extracts prepared following lysis in buffer containing n -octyl β-D-glucopyranoside (see “Materials and methods”).

    Article Snippet: Fluorescence intensity measurements comparing flotillin labeling in different regions of the villous tree were made using the intensity profile tool of the NIS-Elements AR software package (version 3.1, Nikon Instruments).

    Techniques: Nucleic Acid Electrophoresis, Conditioned Place Preference, Expressing, Lysis

    Distribution of FLOT1 and FLOT2 proteins in term human placental villi. (A–D) Representative photomicrographs of conventional cryostat sections (5–6 μm) of placental villi labeled with anti-FLOT1 (HPA001393; red in A) and anti-FLOT2 (1608; red C) antibodies and imaged using laser scanning confocal microscopy. The intensities of these images were adjusted to provide maximal contrast. Panels B and D are corresponding non-confocal differential interference contrast (DIC) micrographs on which DAPI staining (blue) is superimposed. Prominent flotillin labeling (red) is observed in cytotrophoblasts (CTs) and/or basal regions of syncytiotrophoblast (ST), and in fetal capillary endothelial cells (*, lumen of fetal capillary). Arrowheads indicate surface of ST; double arrows indicate flotillin labeling in ST; white single arrows indicate labeling along the basal surface of the ST and/or in CTs; black arrows indicate labeling in endothelial cells. (E, F) Fluorescence intensity measurements for FLOT1 (E) and FLOT2 (F) labeling taken within fetal capillary endothelial cells (EC) of intermediate and terminal villi, and at three levels of the villous tree: terminal villi (TV), intermediate villi (IV), and stem villi (SV). In our analysis, we found that the intensity measurements for FLOT1 EC labeling in intermediate and terminal villi were equivalent, so the data were pooled. This was also the case for FLOT2 EC labeling. Note that the differences in the absolute intensity measurements between FLOT1 and FLOT2 may represent differences in antigen-antibody interactions, and should not be interpreted as differences in relative abundance between these two proteins. Data are mean ± SD from 3 separate term placental specimens; *, P

    Journal: Histochemistry and cell biology

    Article Title: Expression of flotillins in the human placenta: potential implications for placental transcytosis

    doi: 10.1007/s00418-012-1040-2

    Figure Lengend Snippet: Distribution of FLOT1 and FLOT2 proteins in term human placental villi. (A–D) Representative photomicrographs of conventional cryostat sections (5–6 μm) of placental villi labeled with anti-FLOT1 (HPA001393; red in A) and anti-FLOT2 (1608; red C) antibodies and imaged using laser scanning confocal microscopy. The intensities of these images were adjusted to provide maximal contrast. Panels B and D are corresponding non-confocal differential interference contrast (DIC) micrographs on which DAPI staining (blue) is superimposed. Prominent flotillin labeling (red) is observed in cytotrophoblasts (CTs) and/or basal regions of syncytiotrophoblast (ST), and in fetal capillary endothelial cells (*, lumen of fetal capillary). Arrowheads indicate surface of ST; double arrows indicate flotillin labeling in ST; white single arrows indicate labeling along the basal surface of the ST and/or in CTs; black arrows indicate labeling in endothelial cells. (E, F) Fluorescence intensity measurements for FLOT1 (E) and FLOT2 (F) labeling taken within fetal capillary endothelial cells (EC) of intermediate and terminal villi, and at three levels of the villous tree: terminal villi (TV), intermediate villi (IV), and stem villi (SV). In our analysis, we found that the intensity measurements for FLOT1 EC labeling in intermediate and terminal villi were equivalent, so the data were pooled. This was also the case for FLOT2 EC labeling. Note that the differences in the absolute intensity measurements between FLOT1 and FLOT2 may represent differences in antigen-antibody interactions, and should not be interpreted as differences in relative abundance between these two proteins. Data are mean ± SD from 3 separate term placental specimens; *, P

    Article Snippet: Fluorescence intensity measurements comparing flotillin labeling in different regions of the villous tree were made using the intensity profile tool of the NIS-Elements AR software package (version 3.1, Nikon Instruments).

    Techniques: Labeling, Confocal Microscopy, Staining, Fluorescence

    Flotillin expression is downregulated and changes distribution in BeWo cells following foskolin-induced fusion. (A) Representative immunoblots of BeWo cells treated with 20 μM forskolin from 0–72 h. Note that the immunoreactivity of FLOT1 and FLOT2 decreases with forskolin treatment, whereas that of dysferlin (DYSF) increases. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. Similar results were obtained in three separate experiments. (B, C) Mononuclear BeWo cells immunolabeled with antibodies against FLOT1 (HPA001393; red in B) and FLOT2 (1608; red in C), respectively. Double arrows indicate areas of flotillin labeling along cellular boundaries; wide arrows denote areas of periuclear staining in individual cells. (D, E) BeWo cells treated with forskolin (20 μM for 72 h) and co-immunolabeled using antibodies generated against E-cadherin (E-cad, green) and either FLOT1 (red in D) or FLOT2 (red in E), as above. Wide arrows indicate flotillin-like immunoreactivity in crescent-shaped structures in close proximity to clusters of nuclei; arrowhead in E denotes an area of Ecad labeling that has become irregular following cell-cell fusion. (F) Cryosection (5–6 μm) of term placental villus co-immunolabeled using antibodies generated against dysferlin (DYSF, green) and FLOT2 (1608; red) and imaged using confocal microscopy. Single arrows indicate DYSF labeling at the apical regions of the ST; double arrows indicate FLOT2 labeling. (G) BeWo cells treated with forskolin (20 μM for 72 h) and co-immunolabeled using anti-DYSF (green) and anti-FLOT2 (red) antibodies. Arrowheads denote co-labeling in crescent-shaped structures around clusters of nuclei; the asterisk indicates DYSF immunoreactivity in fused cells. Note that non-fused cells lack DYSF labeling. All scale bars = 20 μm

    Journal: Histochemistry and cell biology

    Article Title: Expression of flotillins in the human placenta: potential implications for placental transcytosis

    doi: 10.1007/s00418-012-1040-2

    Figure Lengend Snippet: Flotillin expression is downregulated and changes distribution in BeWo cells following foskolin-induced fusion. (A) Representative immunoblots of BeWo cells treated with 20 μM forskolin from 0–72 h. Note that the immunoreactivity of FLOT1 and FLOT2 decreases with forskolin treatment, whereas that of dysferlin (DYSF) increases. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. Similar results were obtained in three separate experiments. (B, C) Mononuclear BeWo cells immunolabeled with antibodies against FLOT1 (HPA001393; red in B) and FLOT2 (1608; red in C), respectively. Double arrows indicate areas of flotillin labeling along cellular boundaries; wide arrows denote areas of periuclear staining in individual cells. (D, E) BeWo cells treated with forskolin (20 μM for 72 h) and co-immunolabeled using antibodies generated against E-cadherin (E-cad, green) and either FLOT1 (red in D) or FLOT2 (red in E), as above. Wide arrows indicate flotillin-like immunoreactivity in crescent-shaped structures in close proximity to clusters of nuclei; arrowhead in E denotes an area of Ecad labeling that has become irregular following cell-cell fusion. (F) Cryosection (5–6 μm) of term placental villus co-immunolabeled using antibodies generated against dysferlin (DYSF, green) and FLOT2 (1608; red) and imaged using confocal microscopy. Single arrows indicate DYSF labeling at the apical regions of the ST; double arrows indicate FLOT2 labeling. (G) BeWo cells treated with forskolin (20 μM for 72 h) and co-immunolabeled using anti-DYSF (green) and anti-FLOT2 (red) antibodies. Arrowheads denote co-labeling in crescent-shaped structures around clusters of nuclei; the asterisk indicates DYSF immunoreactivity in fused cells. Note that non-fused cells lack DYSF labeling. All scale bars = 20 μm

    Article Snippet: Fluorescence intensity measurements comparing flotillin labeling in different regions of the villous tree were made using the intensity profile tool of the NIS-Elements AR software package (version 3.1, Nikon Instruments).

    Techniques: Expressing, Western Blot, Immunolabeling, Labeling, Staining, Generated, Confocal Microscopy