fluorescein conjugated vicia villosa lectin  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated vicia villosa lectin
    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with <t>Vicia</t> <t>villosa</t> <t>lectin</t> (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).
    Fluorescein Conjugated Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    Images

    1) Product Images from "Genetic ablation of a female specific Apetala 2 transcription factor blocks oocyst shedding in Cryptosporidium parvum"

    Article Title: Genetic ablation of a female specific Apetala 2 transcription factor blocks oocyst shedding in Cryptosporidium parvum

    Journal: bioRxiv

    doi: 10.1101/2022.11.23.517783

    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with Vicia villosa lectin (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).
    Figure Legend Snippet: ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with Vicia villosa lectin (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).

    Techniques Used: Modification, Sequencing, Immunofluorescence, Infection, Labeling, Staining, Expressing, Marker

    fluorescein conjugated vicia villosa lectin  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated vicia villosa lectin
    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with <t>Vicia</t> <t>villosa</t> <t>lectin</t> (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).
    Fluorescein Conjugated Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein conjugated vicia villosa lectin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    Images

    1) Product Images from "Genetic ablation of a female specific Apetala 2 transcription factor blocks oocyst shedding in Cryptosporidium parvum"

    Article Title: Genetic ablation of a female specific Apetala 2 transcription factor blocks oocyst shedding in Cryptosporidium parvum

    Journal: bioRxiv

    doi: 10.1101/2022.11.23.517783

    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with Vicia villosa lectin (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).
    Figure Legend Snippet: ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with Vicia villosa lectin (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).

    Techniques Used: Modification, Sequencing, Immunofluorescence, Infection, Labeling, Staining, Expressing, Marker

    vvl fitc  (Vector Laboratories)


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    Vector Laboratories vvl fitc
    Vvl Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fluorescein label vicia villosa lectin vvl  (Vector Laboratories)


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    Vector Laboratories fluorescein label vicia villosa lectin vvl
    Fluorescein Label Vicia Villosa Lectin Vvl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein label vicia villosa lectin vvl/product/Vector Laboratories
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    fluorescein labeled vicia villosa lectin  (Vector Laboratories)


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    Vector Laboratories fluorescein labeled vicia villosa lectin
    (A) Binding specificities of different lectins used in this study. GNL - Galanthus Nivalis <t>Lectin,</t> SNA - Sambucus Nigra Lectin, VVL - <t>Vicia</t> <t>Villosa</t> Lectin, and CTB - Cholera Toxin B subunit. (B) Western blot analysis of MGAT1 and C1GALT1 expression in KO cells. β-actin levels are shown as loading controls. (C) Comparison of the binding properties of various lectins in WT and KO cells. Representative flow cytometry plots showing differences in lectin binding. Data are representative of at least two independent experiments.
    Fluorescein Labeled Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Avian and Human Influenza Viruses Exhibit Distinct Glycoconjugate Receptor Specificities in Human Lung Cells"

    Article Title: Avian and Human Influenza Viruses Exhibit Distinct Glycoconjugate Receptor Specificities in Human Lung Cells

    Journal: bioRxiv

    doi: 10.1101/2022.06.29.498208

    (A) Binding specificities of different lectins used in this study. GNL - Galanthus Nivalis Lectin, SNA - Sambucus Nigra Lectin, VVL - Vicia Villosa Lectin, and CTB - Cholera Toxin B subunit. (B) Western blot analysis of MGAT1 and C1GALT1 expression in KO cells. β-actin levels are shown as loading controls. (C) Comparison of the binding properties of various lectins in WT and KO cells. Representative flow cytometry plots showing differences in lectin binding. Data are representative of at least two independent experiments.
    Figure Legend Snippet: (A) Binding specificities of different lectins used in this study. GNL - Galanthus Nivalis Lectin, SNA - Sambucus Nigra Lectin, VVL - Vicia Villosa Lectin, and CTB - Cholera Toxin B subunit. (B) Western blot analysis of MGAT1 and C1GALT1 expression in KO cells. β-actin levels are shown as loading controls. (C) Comparison of the binding properties of various lectins in WT and KO cells. Representative flow cytometry plots showing differences in lectin binding. Data are representative of at least two independent experiments.

    Techniques Used: Binding Assay, Western Blot, Expressing, Flow Cytometry

    fluorescein conjugated vva  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated vva
    Fluorescein Conjugated Vva, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fluorescein labelled vicia villosa lectin  (Vector Laboratories)


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    Vector Laboratories fluorescein labelled vicia villosa lectin
    Exemplary illustration of Cryptosporidium parvum in vitro cell cultures at 24 hpi ( A ) <t>Vicia</t> <t>villosa</t> (VV) <t>lectin-based</t> detection of C. parvum (green), illustration of cell membranes via anti-β-catenin-mediated staining (red) and of cell nuclei via DAPI staining (blue). Scale bar 20 µm. ( B ) Infection rates of C. parvum -infected HCT-8 and COLO-680N cells after application of three different infection protocols. Infection rates are expressed as mean ± SD of six replicates per condition. For statistical analysis, a one-way analysis of variance (ANOVA) with Tukey’s test was performed using GraphPad ® Prism 8 software (San Diego, CA, USA), with a significance level of 5%. The significance levels are as follows: *** = p ≤ 0.001, ** = p ≤ 0.01 and presented within the table in ( B ).
    Fluorescein Labelled Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Oesophageal Squamous Cell Carcinoma Cell Line COLO-680N Fails to Support Sustained Cryptosporidium parvum Proliferation"

    Article Title: The Oesophageal Squamous Cell Carcinoma Cell Line COLO-680N Fails to Support Sustained Cryptosporidium parvum Proliferation

    Journal: Pathogens

    doi: 10.3390/pathogens11010049

    Exemplary illustration of Cryptosporidium parvum in vitro cell cultures at 24 hpi ( A ) Vicia villosa (VV) lectin-based detection of C. parvum (green), illustration of cell membranes via anti-β-catenin-mediated staining (red) and of cell nuclei via DAPI staining (blue). Scale bar 20 µm. ( B ) Infection rates of C. parvum -infected HCT-8 and COLO-680N cells after application of three different infection protocols. Infection rates are expressed as mean ± SD of six replicates per condition. For statistical analysis, a one-way analysis of variance (ANOVA) with Tukey’s test was performed using GraphPad ® Prism 8 software (San Diego, CA, USA), with a significance level of 5%. The significance levels are as follows: *** = p ≤ 0.001, ** = p ≤ 0.01 and presented within the table in ( B ).
    Figure Legend Snippet: Exemplary illustration of Cryptosporidium parvum in vitro cell cultures at 24 hpi ( A ) Vicia villosa (VV) lectin-based detection of C. parvum (green), illustration of cell membranes via anti-β-catenin-mediated staining (red) and of cell nuclei via DAPI staining (blue). Scale bar 20 µm. ( B ) Infection rates of C. parvum -infected HCT-8 and COLO-680N cells after application of three different infection protocols. Infection rates are expressed as mean ± SD of six replicates per condition. For statistical analysis, a one-way analysis of variance (ANOVA) with Tukey’s test was performed using GraphPad ® Prism 8 software (San Diego, CA, USA), with a significance level of 5%. The significance levels are as follows: *** = p ≤ 0.001, ** = p ≤ 0.01 and presented within the table in ( B ).

    Techniques Used: In Vitro, Staining, Infection, Software

    Replication of C. parvum in HCT-8- and COLO-680N cells using infection protocol III. Parasite intracellular replication was quantified by both qPCR- ( A ) and Vicia villosa (VV) lectin (VVL)-based immunofluorescence analyses ( B ). ( C ) Exemplary illustration of both in vitro cell culture systems: fluorescence-based detection of C. parvum via VVL (green) and cell nuclei via DAPI (blue). To assess parasite development, a two-tailed t -test was performed, comparing the infection rate per day, measured in HCT-8- and COLO-680N cells. The significance values were as follows: *** = p ≤ 0.001, ** = p ≤ 0.01. Scale bar 20 µm.
    Figure Legend Snippet: Replication of C. parvum in HCT-8- and COLO-680N cells using infection protocol III. Parasite intracellular replication was quantified by both qPCR- ( A ) and Vicia villosa (VV) lectin (VVL)-based immunofluorescence analyses ( B ). ( C ) Exemplary illustration of both in vitro cell culture systems: fluorescence-based detection of C. parvum via VVL (green) and cell nuclei via DAPI (blue). To assess parasite development, a two-tailed t -test was performed, comparing the infection rate per day, measured in HCT-8- and COLO-680N cells. The significance values were as follows: *** = p ≤ 0.001, ** = p ≤ 0.01. Scale bar 20 µm.

    Techniques Used: Infection, Immunofluorescence, In Vitro, Cell Culture, Fluorescence, Two Tailed Test

    fluorescin vicia villosa lectin stain  (Vector Laboratories)


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    Vector Laboratories fluorescin vicia villosa lectin stain
    ( A ) Schematic overview of the chromosomal location for polymorphic gene families in the C. parvum genome. ( B ) Map of the MEDLE2 locus targeted in C. parvum for insertion of a 3× hemagglutinin (HA) epitope tag, a nanoluciferase reporter gene (Nluc), and neomycin phosphotransferase selection marker (Neo). ( C ) PCR mapping of the MEDLE2 locus using genomic DNA from wild type (WT) and transgenic (MEDLE2-HA) sporozoites, corresponding primer pairs are shown in ( B ), and thymidine kinase (TK) gene used as a control. Note the presence of two bands in the 5′–3′ amplification, indicating the presence of a transgene (3081 bp) and persistence of an unmodified copy (1174 bp), suggesting multiple copies of MEDLE2 in the C. parvum genome; also see . ( D, E ) HCT-8 cultures were infected with WYLE4-HA ( D ) or MEDLE2-HA ( E ) transgenic parasites and fixed after 24 hr for immunofluorescence assay (IFA). Red, antibody to HA; green, <t>Vicia</t> <t>villosa</t> <t>lectin</t> stain, VVL ; blue, Hoechst DNA dye. Additional genes targeted and the localizations of their products are summarized in and and .
    Fluorescin Vicia Villosa Lectin Stain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The enteric pathogen Cryptosporidium parvum exports proteins into the cytosol of the infected host cell"

    Article Title: The enteric pathogen Cryptosporidium parvum exports proteins into the cytosol of the infected host cell

    Journal: eLife

    doi: 10.7554/eLife.70451

    ( A ) Schematic overview of the chromosomal location for polymorphic gene families in the C. parvum genome. ( B ) Map of the MEDLE2 locus targeted in C. parvum for insertion of a 3× hemagglutinin (HA) epitope tag, a nanoluciferase reporter gene (Nluc), and neomycin phosphotransferase selection marker (Neo). ( C ) PCR mapping of the MEDLE2 locus using genomic DNA from wild type (WT) and transgenic (MEDLE2-HA) sporozoites, corresponding primer pairs are shown in ( B ), and thymidine kinase (TK) gene used as a control. Note the presence of two bands in the 5′–3′ amplification, indicating the presence of a transgene (3081 bp) and persistence of an unmodified copy (1174 bp), suggesting multiple copies of MEDLE2 in the C. parvum genome; also see . ( D, E ) HCT-8 cultures were infected with WYLE4-HA ( D ) or MEDLE2-HA ( E ) transgenic parasites and fixed after 24 hr for immunofluorescence assay (IFA). Red, antibody to HA; green, Vicia villosa lectin stain, VVL ; blue, Hoechst DNA dye. Additional genes targeted and the localizations of their products are summarized in and and .
    Figure Legend Snippet: ( A ) Schematic overview of the chromosomal location for polymorphic gene families in the C. parvum genome. ( B ) Map of the MEDLE2 locus targeted in C. parvum for insertion of a 3× hemagglutinin (HA) epitope tag, a nanoluciferase reporter gene (Nluc), and neomycin phosphotransferase selection marker (Neo). ( C ) PCR mapping of the MEDLE2 locus using genomic DNA from wild type (WT) and transgenic (MEDLE2-HA) sporozoites, corresponding primer pairs are shown in ( B ), and thymidine kinase (TK) gene used as a control. Note the presence of two bands in the 5′–3′ amplification, indicating the presence of a transgene (3081 bp) and persistence of an unmodified copy (1174 bp), suggesting multiple copies of MEDLE2 in the C. parvum genome; also see . ( D, E ) HCT-8 cultures were infected with WYLE4-HA ( D ) or MEDLE2-HA ( E ) transgenic parasites and fixed after 24 hr for immunofluorescence assay (IFA). Red, antibody to HA; green, Vicia villosa lectin stain, VVL ; blue, Hoechst DNA dye. Additional genes targeted and the localizations of their products are summarized in and and .

    Techniques Used: Selection, Marker, Transgenic Assay, Amplification, Infection, Immunofluorescence, Staining

    fluorescein vicia villosa lectin vva  (Vector Laboratories)


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    Vector Laboratories fluorescein vicia villosa lectin vva
    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with <t>Vicia</t> <t>villosa</t> <t>lectin</t> <t>(VVA;</t> green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.
    Fluorescein Vicia Villosa Lectin Vva, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein vicia villosa lectin vva/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    fluorescein vicia villosa lectin vva - by Bioz Stars, 2024-06
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    Images

    1) Product Images from "Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure"

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    Journal: eLife

    doi: 10.7554/eLife.55965

    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.
    Figure Legend Snippet: ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Techniques Used: In Situ Hybridization, Expressing, Labeling, Staining


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation, Construct, Recombinant, Sequencing, Software

    fluoresceinvicia villosa lectin vva  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluoresceinvicia villosa lectin vva
    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia <t>villosa</t> <t>lectin</t> <t>(VVA;</t> green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.
    Fluoresceinvicia Villosa Lectin Vva, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure"

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    Journal: eLife

    doi: 10.7554/eLife.55965

    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.
    Figure Legend Snippet: ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Techniques Used: In Situ Hybridization, Expressing, Labeling, Staining


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation, Construct, Recombinant, Sequencing, Software

    fluorescein conjugated vicia villosa lectin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein conjugated vicia villosa lectin
    a,b , HCT-8 cells were infected with COWP1–HA C. parvum and after 36 h of infection, the nucleotide analogue EdU was added to the medium. Then, 12 h later, cells were click labelled and counterstained with anti-HA antibodies or <t>Vicia</t> <t>villosa</t> <t>lectin</t> (VVL; a ). Cells were scored for nuclear EdU labelling ( b ); 100 stages were quantified for three biological replicates, and the experiment was performed twice. Data are mean ± s.d. c , Representative images of encounters between male and female gametes in culture; gametes were identified using the indicated transgenes or antibodies, and attached males are highlighted by arrowheads. YFP, yellow fluorescent protein. d – h , Ifng −/− mice were infected with H2B–mNeon-expressing ( d ) or COWP1–HA-expressing ( g , h ) parasites, and intestines were sectioned and prepared for immunofluorescence assays and counterstained with anti-TrpB, anti-H3K9Ac or anti-RAD51 antibodies. Representative micrographs show progression of events following fertilization. Post-fertilization stages are abundant in vivo ( e ) and these stages were significantly larger than those found in vitro ( f ); each symbol represents a parasite, n = 25. For e , data are mean ± s.d. from three independent mice. For f , data are mean ± s.d.; the statistical analysis was performed using a two-sided Student’s t -test comparing cultured females with in vivo females (** P = 0.0023) or with in vivo oocysts (**** P = 0.0001). All of the microscopy experiments shown in d – h were performed twice with similar results. Scale bars, 1 µm.
    Fluorescein Conjugated Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Life cycle progression and sexual development of the apicomplexan parasite Cryptosporidium parvum"

    Article Title: Life cycle progression and sexual development of the apicomplexan parasite Cryptosporidium parvum

    Journal: Nature Microbiology

    doi: 10.1038/s41564-019-0539-x

    a,b , HCT-8 cells were infected with COWP1–HA C. parvum and after 36 h of infection, the nucleotide analogue EdU was added to the medium. Then, 12 h later, cells were click labelled and counterstained with anti-HA antibodies or Vicia villosa lectin (VVL; a ). Cells were scored for nuclear EdU labelling ( b ); 100 stages were quantified for three biological replicates, and the experiment was performed twice. Data are mean ± s.d. c , Representative images of encounters between male and female gametes in culture; gametes were identified using the indicated transgenes or antibodies, and attached males are highlighted by arrowheads. YFP, yellow fluorescent protein. d – h , Ifng −/− mice were infected with H2B–mNeon-expressing ( d ) or COWP1–HA-expressing ( g , h ) parasites, and intestines were sectioned and prepared for immunofluorescence assays and counterstained with anti-TrpB, anti-H3K9Ac or anti-RAD51 antibodies. Representative micrographs show progression of events following fertilization. Post-fertilization stages are abundant in vivo ( e ) and these stages were significantly larger than those found in vitro ( f ); each symbol represents a parasite, n = 25. For e , data are mean ± s.d. from three independent mice. For f , data are mean ± s.d.; the statistical analysis was performed using a two-sided Student’s t -test comparing cultured females with in vivo females (** P = 0.0023) or with in vivo oocysts (**** P = 0.0001). All of the microscopy experiments shown in d – h were performed twice with similar results. Scale bars, 1 µm.
    Figure Legend Snippet: a,b , HCT-8 cells were infected with COWP1–HA C. parvum and after 36 h of infection, the nucleotide analogue EdU was added to the medium. Then, 12 h later, cells were click labelled and counterstained with anti-HA antibodies or Vicia villosa lectin (VVL; a ). Cells were scored for nuclear EdU labelling ( b ); 100 stages were quantified for three biological replicates, and the experiment was performed twice. Data are mean ± s.d. c , Representative images of encounters between male and female gametes in culture; gametes were identified using the indicated transgenes or antibodies, and attached males are highlighted by arrowheads. YFP, yellow fluorescent protein. d – h , Ifng −/− mice were infected with H2B–mNeon-expressing ( d ) or COWP1–HA-expressing ( g , h ) parasites, and intestines were sectioned and prepared for immunofluorescence assays and counterstained with anti-TrpB, anti-H3K9Ac or anti-RAD51 antibodies. Representative micrographs show progression of events following fertilization. Post-fertilization stages are abundant in vivo ( e ) and these stages were significantly larger than those found in vitro ( f ); each symbol represents a parasite, n = 25. For e , data are mean ± s.d. from three independent mice. For f , data are mean ± s.d.; the statistical analysis was performed using a two-sided Student’s t -test comparing cultured females with in vivo females (** P = 0.0023) or with in vivo oocysts (**** P = 0.0001). All of the microscopy experiments shown in d – h were performed twice with similar results. Scale bars, 1 µm.

    Techniques Used: Infection, Expressing, Immunofluorescence, In Vivo, In Vitro, Cell Culture, Microscopy

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    Vector Laboratories fluorescein conjugated vicia villosa lectin
    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with <t>Vicia</t> <t>villosa</t> <t>lectin</t> (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).
    Fluorescein Conjugated Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Vector Laboratories vvl fitc
    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with <t>Vicia</t> <t>villosa</t> <t>lectin</t> (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).
    Vvl Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Vector Laboratories fluorescein label vicia villosa lectin vvl
    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with <t>Vicia</t> <t>villosa</t> <t>lectin</t> (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).
    Fluorescein Label Vicia Villosa Lectin Vvl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Vector Laboratories fluorescein labeled vicia villosa lectin
    (A) Binding specificities of different lectins used in this study. GNL - Galanthus Nivalis <t>Lectin,</t> SNA - Sambucus Nigra Lectin, VVL - <t>Vicia</t> <t>Villosa</t> Lectin, and CTB - Cholera Toxin B subunit. (B) Western blot analysis of MGAT1 and C1GALT1 expression in KO cells. β-actin levels are shown as loading controls. (C) Comparison of the binding properties of various lectins in WT and KO cells. Representative flow cytometry plots showing differences in lectin binding. Data are representative of at least two independent experiments.
    Fluorescein Labeled Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein labeled vicia villosa lectin/product/Vector Laboratories
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    Vector Laboratories fluorescein conjugated vva
    (A) Binding specificities of different lectins used in this study. GNL - Galanthus Nivalis <t>Lectin,</t> SNA - Sambucus Nigra Lectin, VVL - <t>Vicia</t> <t>Villosa</t> Lectin, and CTB - Cholera Toxin B subunit. (B) Western blot analysis of MGAT1 and C1GALT1 expression in KO cells. β-actin levels are shown as loading controls. (C) Comparison of the binding properties of various lectins in WT and KO cells. Representative flow cytometry plots showing differences in lectin binding. Data are representative of at least two independent experiments.
    Fluorescein Conjugated Vva, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories fluorescein labelled vicia villosa lectin
    Exemplary illustration of Cryptosporidium parvum in vitro cell cultures at 24 hpi ( A ) <t>Vicia</t> <t>villosa</t> (VV) <t>lectin-based</t> detection of C. parvum (green), illustration of cell membranes via anti-β-catenin-mediated staining (red) and of cell nuclei via DAPI staining (blue). Scale bar 20 µm. ( B ) Infection rates of C. parvum -infected HCT-8 and COLO-680N cells after application of three different infection protocols. Infection rates are expressed as mean ± SD of six replicates per condition. For statistical analysis, a one-way analysis of variance (ANOVA) with Tukey’s test was performed using GraphPad ® Prism 8 software (San Diego, CA, USA), with a significance level of 5%. The significance levels are as follows: *** = p ≤ 0.001, ** = p ≤ 0.01 and presented within the table in ( B ).
    Fluorescein Labelled Vicia Villosa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories fluorescin vicia villosa lectin stain
    ( A ) Schematic overview of the chromosomal location for polymorphic gene families in the C. parvum genome. ( B ) Map of the MEDLE2 locus targeted in C. parvum for insertion of a 3× hemagglutinin (HA) epitope tag, a nanoluciferase reporter gene (Nluc), and neomycin phosphotransferase selection marker (Neo). ( C ) PCR mapping of the MEDLE2 locus using genomic DNA from wild type (WT) and transgenic (MEDLE2-HA) sporozoites, corresponding primer pairs are shown in ( B ), and thymidine kinase (TK) gene used as a control. Note the presence of two bands in the 5′–3′ amplification, indicating the presence of a transgene (3081 bp) and persistence of an unmodified copy (1174 bp), suggesting multiple copies of MEDLE2 in the C. parvum genome; also see . ( D, E ) HCT-8 cultures were infected with WYLE4-HA ( D ) or MEDLE2-HA ( E ) transgenic parasites and fixed after 24 hr for immunofluorescence assay (IFA). Red, antibody to HA; green, <t>Vicia</t> <t>villosa</t> <t>lectin</t> stain, VVL ; blue, Hoechst DNA dye. Additional genes targeted and the localizations of their products are summarized in and and .
    Fluorescin Vicia Villosa Lectin Stain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories fluorescein vicia villosa lectin vva
    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with <t>Vicia</t> <t>villosa</t> <t>lectin</t> <t>(VVA;</t> green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.
    Fluorescein Vicia Villosa Lectin Vva, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories fluoresceinvicia villosa lectin vva
    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia <t>villosa</t> <t>lectin</t> <t>(VVA;</t> green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.
    Fluoresceinvicia Villosa Lectin Vva, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with Vicia villosa lectin (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).

    Journal: bioRxiv

    Article Title: Genetic ablation of a female specific Apetala 2 transcription factor blocks oocyst shedding in Cryptosporidium parvum

    doi: 10.1101/2022.11.23.517783

    Figure Lengend Snippet: ( A ) The locus of cgd4_1110 was modified to append a 3xHA epitope tag to the coding sequence. The resulting parasite strain was used to infect HCT-8 cell cultures and analyzed by immunofluorescence assays (IFA) at 24 and 48h post infection. The transgene was detected with antibodies to HA (red) and all parasites were labeled with Vicia villosa lectin (VVL, green). ( B ) Quantification of HA staining as a proportion of all parasites. ( C ) IFA of cultures infected with cgd4_1110-mNG showing asexual meronts, a female gamete, and a male gamont at 48h. mNeon Green (mNG) fluorescent protein is shown in green, DNA stained with DAPI in blue, and TrpB (labeling all parasites) in red. The single nucleus of the female gamete is highlighted with an arrowhead. ( D ) cgd4_1110-3xHA (red) expressing parasites stained for the female marker DMC1 (note that all HA positive parasites are also DMC1 positive).

    Article Snippet: Parasites were counter-stained with anti-HA antibody and fluorescein conjugated Vicia villosa lectin (Vector Laboratories).

    Techniques: Modification, Sequencing, Immunofluorescence, Infection, Labeling, Staining, Expressing, Marker

    (A) Binding specificities of different lectins used in this study. GNL - Galanthus Nivalis Lectin, SNA - Sambucus Nigra Lectin, VVL - Vicia Villosa Lectin, and CTB - Cholera Toxin B subunit. (B) Western blot analysis of MGAT1 and C1GALT1 expression in KO cells. β-actin levels are shown as loading controls. (C) Comparison of the binding properties of various lectins in WT and KO cells. Representative flow cytometry plots showing differences in lectin binding. Data are representative of at least two independent experiments.

    Journal: bioRxiv

    Article Title: Avian and Human Influenza Viruses Exhibit Distinct Glycoconjugate Receptor Specificities in Human Lung Cells

    doi: 10.1101/2022.06.29.498208

    Figure Lengend Snippet: (A) Binding specificities of different lectins used in this study. GNL - Galanthus Nivalis Lectin, SNA - Sambucus Nigra Lectin, VVL - Vicia Villosa Lectin, and CTB - Cholera Toxin B subunit. (B) Western blot analysis of MGAT1 and C1GALT1 expression in KO cells. β-actin levels are shown as loading controls. (C) Comparison of the binding properties of various lectins in WT and KO cells. Representative flow cytometry plots showing differences in lectin binding. Data are representative of at least two independent experiments.

    Article Snippet: Fluorescein labeled Galanthus Nivalis Lectin (GNL-FITC, #FL-1241 1:250), Cy3 labeled Sambucus Nigra Lectin (SNA-Cy3, #CL-1303, 1:500), and fluorescein labeled Vicia Villosa Lectin (VVL-FITC, #FL-1231, 1:500) were purchased from Vector Laboratories.

    Techniques: Binding Assay, Western Blot, Expressing, Flow Cytometry

    Exemplary illustration of Cryptosporidium parvum in vitro cell cultures at 24 hpi ( A ) Vicia villosa (VV) lectin-based detection of C. parvum (green), illustration of cell membranes via anti-β-catenin-mediated staining (red) and of cell nuclei via DAPI staining (blue). Scale bar 20 µm. ( B ) Infection rates of C. parvum -infected HCT-8 and COLO-680N cells after application of three different infection protocols. Infection rates are expressed as mean ± SD of six replicates per condition. For statistical analysis, a one-way analysis of variance (ANOVA) with Tukey’s test was performed using GraphPad ® Prism 8 software (San Diego, CA, USA), with a significance level of 5%. The significance levels are as follows: *** = p ≤ 0.001, ** = p ≤ 0.01 and presented within the table in ( B ).

    Journal: Pathogens

    Article Title: The Oesophageal Squamous Cell Carcinoma Cell Line COLO-680N Fails to Support Sustained Cryptosporidium parvum Proliferation

    doi: 10.3390/pathogens11010049

    Figure Lengend Snippet: Exemplary illustration of Cryptosporidium parvum in vitro cell cultures at 24 hpi ( A ) Vicia villosa (VV) lectin-based detection of C. parvum (green), illustration of cell membranes via anti-β-catenin-mediated staining (red) and of cell nuclei via DAPI staining (blue). Scale bar 20 µm. ( B ) Infection rates of C. parvum -infected HCT-8 and COLO-680N cells after application of three different infection protocols. Infection rates are expressed as mean ± SD of six replicates per condition. For statistical analysis, a one-way analysis of variance (ANOVA) with Tukey’s test was performed using GraphPad ® Prism 8 software (San Diego, CA, USA), with a significance level of 5%. The significance levels are as follows: *** = p ≤ 0.001, ** = p ≤ 0.01 and presented within the table in ( B ).

    Article Snippet: For detection of intracellular parasites and posterior calculation of infection rates, staining with the fluorescein labelled Vicia villosa lectin (VVL, FL-1231-2, 1:2000, VECTOR laboratories, 45 min, room temperature (RT), dark chamber) was used.

    Techniques: In Vitro, Staining, Infection, Software

    Replication of C. parvum in HCT-8- and COLO-680N cells using infection protocol III. Parasite intracellular replication was quantified by both qPCR- ( A ) and Vicia villosa (VV) lectin (VVL)-based immunofluorescence analyses ( B ). ( C ) Exemplary illustration of both in vitro cell culture systems: fluorescence-based detection of C. parvum via VVL (green) and cell nuclei via DAPI (blue). To assess parasite development, a two-tailed t -test was performed, comparing the infection rate per day, measured in HCT-8- and COLO-680N cells. The significance values were as follows: *** = p ≤ 0.001, ** = p ≤ 0.01. Scale bar 20 µm.

    Journal: Pathogens

    Article Title: The Oesophageal Squamous Cell Carcinoma Cell Line COLO-680N Fails to Support Sustained Cryptosporidium parvum Proliferation

    doi: 10.3390/pathogens11010049

    Figure Lengend Snippet: Replication of C. parvum in HCT-8- and COLO-680N cells using infection protocol III. Parasite intracellular replication was quantified by both qPCR- ( A ) and Vicia villosa (VV) lectin (VVL)-based immunofluorescence analyses ( B ). ( C ) Exemplary illustration of both in vitro cell culture systems: fluorescence-based detection of C. parvum via VVL (green) and cell nuclei via DAPI (blue). To assess parasite development, a two-tailed t -test was performed, comparing the infection rate per day, measured in HCT-8- and COLO-680N cells. The significance values were as follows: *** = p ≤ 0.001, ** = p ≤ 0.01. Scale bar 20 µm.

    Article Snippet: For detection of intracellular parasites and posterior calculation of infection rates, staining with the fluorescein labelled Vicia villosa lectin (VVL, FL-1231-2, 1:2000, VECTOR laboratories, 45 min, room temperature (RT), dark chamber) was used.

    Techniques: Infection, Immunofluorescence, In Vitro, Cell Culture, Fluorescence, Two Tailed Test

    ( A ) Schematic overview of the chromosomal location for polymorphic gene families in the C. parvum genome. ( B ) Map of the MEDLE2 locus targeted in C. parvum for insertion of a 3× hemagglutinin (HA) epitope tag, a nanoluciferase reporter gene (Nluc), and neomycin phosphotransferase selection marker (Neo). ( C ) PCR mapping of the MEDLE2 locus using genomic DNA from wild type (WT) and transgenic (MEDLE2-HA) sporozoites, corresponding primer pairs are shown in ( B ), and thymidine kinase (TK) gene used as a control. Note the presence of two bands in the 5′–3′ amplification, indicating the presence of a transgene (3081 bp) and persistence of an unmodified copy (1174 bp), suggesting multiple copies of MEDLE2 in the C. parvum genome; also see . ( D, E ) HCT-8 cultures were infected with WYLE4-HA ( D ) or MEDLE2-HA ( E ) transgenic parasites and fixed after 24 hr for immunofluorescence assay (IFA). Red, antibody to HA; green, Vicia villosa lectin stain, VVL ; blue, Hoechst DNA dye. Additional genes targeted and the localizations of their products are summarized in and and .

    Journal: eLife

    Article Title: The enteric pathogen Cryptosporidium parvum exports proteins into the cytosol of the infected host cell

    doi: 10.7554/eLife.70451

    Figure Lengend Snippet: ( A ) Schematic overview of the chromosomal location for polymorphic gene families in the C. parvum genome. ( B ) Map of the MEDLE2 locus targeted in C. parvum for insertion of a 3× hemagglutinin (HA) epitope tag, a nanoluciferase reporter gene (Nluc), and neomycin phosphotransferase selection marker (Neo). ( C ) PCR mapping of the MEDLE2 locus using genomic DNA from wild type (WT) and transgenic (MEDLE2-HA) sporozoites, corresponding primer pairs are shown in ( B ), and thymidine kinase (TK) gene used as a control. Note the presence of two bands in the 5′–3′ amplification, indicating the presence of a transgene (3081 bp) and persistence of an unmodified copy (1174 bp), suggesting multiple copies of MEDLE2 in the C. parvum genome; also see . ( D, E ) HCT-8 cultures were infected with WYLE4-HA ( D ) or MEDLE2-HA ( E ) transgenic parasites and fixed after 24 hr for immunofluorescence assay (IFA). Red, antibody to HA; green, Vicia villosa lectin stain, VVL ; blue, Hoechst DNA dye. Additional genes targeted and the localizations of their products are summarized in and and .

    Article Snippet: Other , Fluorescin Vicia villosa lectin stain , Vector Labs , Cat# FL-1231-2 , IF (1:1000).

    Techniques: Selection, Marker, Transgenic Assay, Amplification, Infection, Immunofluorescence, Staining

    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Journal: eLife

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    doi: 10.7554/eLife.55965

    Figure Lengend Snippet: ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Article Snippet: The following primary antibodies were used: rat anti-alpha tubulin (tyrosinated) 1:500 (MAB 1864-I, MilliporeSigma), mouse anti-alpha tubulin (acetylated) 1:500 (T6793, Sigma-Aldrich), rat anti-Ecadherin 1:500 (DCAD2, DSHB), mouse anti-Fasciclin III 1:500 (7G10, DSHB), rabbit anti-histone H3 (phospho S10) 1:50 (ab5176, Abcam), goat anti-GFP 1:300 (ab6662, Abcam), fluorescein Vicia Villosa Lectin (VVA) 1:200 (FL-1231, Vector Laboratories).

    Techniques: In Situ Hybridization, Expressing, Labeling, Staining

    Journal: eLife

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    doi: 10.7554/eLife.55965

    Figure Lengend Snippet:

    Article Snippet: The following primary antibodies were used: rat anti-alpha tubulin (tyrosinated) 1:500 (MAB 1864-I, MilliporeSigma), mouse anti-alpha tubulin (acetylated) 1:500 (T6793, Sigma-Aldrich), rat anti-Ecadherin 1:500 (DCAD2, DSHB), mouse anti-Fasciclin III 1:500 (7G10, DSHB), rabbit anti-histone H3 (phospho S10) 1:50 (ab5176, Abcam), goat anti-GFP 1:300 (ab6662, Abcam), fluorescein Vicia Villosa Lectin (VVA) 1:200 (FL-1231, Vector Laboratories).

    Techniques: Plasmid Preparation, Construct, Recombinant, Sequencing, Software

    ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Journal: eLife

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    doi: 10.7554/eLife.55965

    Figure Lengend Snippet: ( A–B ) in situ hybridization for dumpy mRNA in the lobed species D. melanogaster ( A ) and the non-lobed species D. biarmipes ( B ). Pink box outlines location of zoomed in images presented in A1 and B1. Relevant expression highlighted with arrow (purple/white) for strong expression, asterisk for weak expression, and arrowhead for clasper-specific expression. Expression observed in D. melanogaster at 44 hr APF is not present in all samples (see ). ( C–D ) aECM is labeled with Vicia villosa lectin (VVA; green) and apical membrane labeled with E-cadherin (Ecad; magenta) at 44 hr APF in D. melanogaster ( C ) and D. biarmipes ( D ). Location of respective cross sections indicated in yellow for lateral plate ( C2–D2 ) and blue for posterior lobe in D. melanogaster ( C1 ) and corresponding position in D. biarmipes ( D1 ). All cross-sections are oriented with apical side at the top and basal side at the bottom. White arrows highlight the crevice localization between the lateral plate and clasper, which the aECM fills in D. melanogaster ( C1 ), but only a weakly stained strand-like structure of aECM appears in D. biarmipes ( D1 ). Tendrils of aECM can also be observed connecting to the lateral plate in both species (red arrowheads). Relevant structures labeled: Posterior lobe (PL), lateral plate (LP), clasper (C), sheath (S), and phallus (P). Scale bar, 20 μm. n = at least five per experiment.

    Article Snippet: Lectin , fluoresceinVicia Villosa Lectin (VVA) , Vector Laboratories , Vector Laboratories Cat# FL-1231, RRID: AB_2336856 , IHC (1:200).

    Techniques: In Situ Hybridization, Expressing, Labeling, Staining

    Journal: eLife

    Article Title: Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure

    doi: 10.7554/eLife.55965

    Figure Lengend Snippet:

    Article Snippet: Lectin , fluoresceinVicia Villosa Lectin (VVA) , Vector Laboratories , Vector Laboratories Cat# FL-1231, RRID: AB_2336856 , IHC (1:200).

    Techniques: Plasmid Preparation, Construct, Recombinant, Sequencing, Software