fluorescein fitc affinipure fab fragment goat anti rabbit igg  (Jackson Immuno)

 
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    Name:
    Fluorescein FITC AffiniPure Fab Fragment Goat Anti Rabbit IgG
    Description:
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    111-097-003
    Price:
    119.0
    Category:
    Fab Fragment Affinity‑Purified Antibodies
    Conjugate:
    Fluorescein FITC
    Size:
    1 0 mg
    Format:
    Fab Fragment
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Jackson Immuno fluorescein fitc affinipure fab fragment goat anti rabbit igg
    Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and <t>FITC-conjugated</t> secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control <t>IgG</t> binding (fold enrichment) was assessed using the two-tailed Student's t -test.
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/fluorescein fitc affinipure fab fragment goat anti rabbit igg/product/Jackson Immuno
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein fitc affinipure fab fragment goat anti rabbit igg - by Bioz Stars, 2021-07
    88/100 stars

    Images

    1) Product Images from "Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases"

    Article Title: Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases

    Journal: Biology Open

    doi: 10.1242/bio.019729

    Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and FITC-conjugated secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control IgG binding (fold enrichment) was assessed using the two-tailed Student's t -test.
    Figure Legend Snippet: Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and FITC-conjugated secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control IgG binding (fold enrichment) was assessed using the two-tailed Student's t -test.

    Techniques Used: Transfection, Luciferase, Staining, Isolation, Western Blot, Methylation, Standard Deviation, Two Tailed Test, Chromatin Immunoprecipitation, Sonication, Purification, Real-time Polymerase Chain Reaction, Binding Assay

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    Article Title: Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons
    Article Snippet: This incubation was followed by three washes in PBS/0.1%TX and a longer incubation (10 min) in PBS. .. The slides were then incubated in FITC-AffiniPure Fab Fragment Goat Anti-Rabbit IgG (Jackson ImmunoResearch) diluted 1:50 in PBS/0.1%TX, for 2 h at RT. .. This incubation was followed by five 5 min washes in PBS/0.1%TX followed by a 60 min wash in PBS, then the slides were blocked overnight with a nonconjugated AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) diluted 1:10 in PBS/0.1%TX.

    Article Title: Functional Live Cell Imaging of the Pulmonary Neuroepithelial Body Microenvironment
    Article Snippet: .. Sections were then incubated for a consecutive night with a second primary antibody against protein-gene product 9.5 (PGP9.5, 1:4,000, 7863-0507, Rb Pc; Biogenesis, Poole, UK), a pan-neuronal and neuroendocrine marker, followed by a 4-hour secondary incubation with fluorescein isothiocyanate (FITC)-conjugated Fab fragments of goat anti-rabbit immunoglobulins (GAR-Fab-FITC, 1:100, 111-097-003; Jackson ImmunoResearch). ..

    Marker:

    Article Title: Functional Live Cell Imaging of the Pulmonary Neuroepithelial Body Microenvironment
    Article Snippet: .. Sections were then incubated for a consecutive night with a second primary antibody against protein-gene product 9.5 (PGP9.5, 1:4,000, 7863-0507, Rb Pc; Biogenesis, Poole, UK), a pan-neuronal and neuroendocrine marker, followed by a 4-hour secondary incubation with fluorescein isothiocyanate (FITC)-conjugated Fab fragments of goat anti-rabbit immunoglobulins (GAR-Fab-FITC, 1:100, 111-097-003; Jackson ImmunoResearch). ..

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  • 88
    Jackson Immuno fluorescein fitc affinipure fab fragment goat anti rabbit igg
    Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and <t>FITC-conjugated</t> secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control <t>IgG</t> binding (fold enrichment) was assessed using the two-tailed Student's t -test.
    Fluorescein Fitc Affinipure Fab Fragment Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein fitc affinipure fab fragment goat anti rabbit igg/product/Jackson Immuno
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein fitc affinipure fab fragment goat anti rabbit igg - by Bioz Stars, 2021-07
    88/100 stars
      Buy from Supplier

    85
    Jackson Immuno rabbit f ab fitc anti goat igg h l
    Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and <t>FITC-conjugated</t> secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control <t>IgG</t> binding (fold enrichment) was assessed using the two-tailed Student's t -test.
    Rabbit F Ab Fitc Anti Goat Igg H L, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit f ab fitc anti goat igg h l/product/Jackson Immuno
    Average 85 stars, based on 1 article reviews
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    rabbit f ab fitc anti goat igg h l - by Bioz Stars, 2021-07
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    94
    Jackson Immuno goat anti rabbit igg fab fragments conjugated to fitc
    Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and <t>FITC-conjugated</t> secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control <t>IgG</t> binding (fold enrichment) was assessed using the two-tailed Student's t -test.
    Goat Anti Rabbit Igg Fab Fragments Conjugated To Fitc, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg fab fragments conjugated to fitc/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg fab fragments conjugated to fitc - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and FITC-conjugated secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control IgG binding (fold enrichment) was assessed using the two-tailed Student's t -test.

    Journal: Biology Open

    Article Title: Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases

    doi: 10.1242/bio.019729

    Figure Lengend Snippet: Inactivation of the MLL complex prevents the recruitment of MCM2-7 proteins onto chromatin. (A) HCT116 cells were transfected with 50 nM siRNAs of luciferase, Geminin, Geminin+RBBP5, CDT2, CDT2+RBBP5 and RBBP5. The cells were fixed and stained with anti-MCM7 and FITC-conjugated secondary antibodies and counter-stained with DAPI. Scale bars: 50 μm. (B) Proteins from the siRNA-treated cell lysates in A were analyzed by specific antibodies as indicated. (C) Downregulation of WDR5 or RBBP5 reduces the recruitment of MCM proteins to chromatin. Chromatin fractions were isolated from control and WDR5 or RBBP5 siRNA-treated cells and the chromatin-associated MCM proteins were examined by western blotting as indicated. (D) Relative protein levels of MCMs and methylated histone H3K4 on chromatin in C were quantified using Gel-Pro analyzer 4.0. The error bars indicate standard deviation of triplicated samples. The statistical differences of MCM2, MCM3, MCM7, CDT1 and histone H3K4 methylations between control and specific siRNA samples were analyzed using the two-tailed Student's t -test. (E,F) The chromatin immunoprecipitation (ChIP) analysis was performed to locate MCM7 (E) and MCM2 (F) proteins on the DNA replication origin at the Mcm4 gene. Proteins were cross-linked to chromatin and chromatin DNA was sonicated to generate 500-1000 base-pairs (bps) fragments in average length. The ChIP-grade anti-MCM7 and MCM2 antibodies were used for chromatin immunoprecipitation. Cross-linked DNA was released, purified, and analyzed for the enrichment of DNA fragments associated with MCM2 and MCM7 from −4.0 kb to 4.0 kb (kilobase pairs) along the Mcm4 region using various Mcm4 primers and quantitative real time PCR as described in the Materials and Methods. Error bars indicate the standard deviation of triplicated samples. The statistical significance of antibody-enriched specific Mcm4 DNA sequences over the background control IgG binding (fold enrichment) was assessed using the two-tailed Student's t -test.

    Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated rabbit secondary antibodies (111-097-003) were from Jackson ImmunoResearch Laboratories (West Grove, PA).

    Techniques: Transfection, Luciferase, Staining, Isolation, Western Blot, Methylation, Standard Deviation, Two Tailed Test, Chromatin Immunoprecipitation, Sonication, Purification, Real-time Polymerase Chain Reaction, Binding Assay