fluorescein 12 dutp solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fluorescein 12 dutp solution
    Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of <t>fluorescein-12-dUTP</t> during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.
    Fluorescein 12 Dutp Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 dutp solution/product/Thermo Fisher
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp solution - by Bioz Stars, 2022-10
    94/100 stars

    Images

    1) Product Images from "TUNEL Assay to Assess Extent of DNA Fragmentation and Programmed Cell Death in Root Cells under Various Stress Conditions"

    Article Title: TUNEL Assay to Assess Extent of DNA Fragmentation and Programmed Cell Death in Root Cells under Various Stress Conditions

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.2502

    Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of fluorescein-12-dUTP during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.
    Figure Legend Snippet: Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of fluorescein-12-dUTP during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.

    Techniques Used: TUNEL Assay, In Situ, Microscopy, Software, Staining, Fluorescence

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    Thermo Fisher fluorescein 12 dutp solution
    Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of <t>fluorescein-12-dUTP</t> during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.
    Fluorescein 12 Dutp Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 dutp solution/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp solution - by Bioz Stars, 2022-10
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher fluorescein 12 dutp
    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with <t>fluorescein-12-dUTP</t> (green).
    Fluorescein 12 Dutp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 dutp/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of fluorescein-12-dUTP during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.

    Journal: Bio-protocol

    Article Title: TUNEL Assay to Assess Extent of DNA Fragmentation and Programmed Cell Death in Root Cells under Various Stress Conditions

    doi: 10.21769/BioProtoc.2502

    Figure Lengend Snippet: Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of fluorescein-12-dUTP during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.

    Article Snippet: Fluorescein-12-dUTP* (Thermo Fisher Scientific, Thermo ScientificTM, catalog number: R0101). dATP* (Promega, catalog number: U1202).

    Techniques: TUNEL Assay, In Situ, Microscopy, Software, Staining, Fluorescence

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Journal: Scientific Reports

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    doi: 10.1038/s41598-018-34545-x

    Figure Lengend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Article Snippet: L. mollis genomic DNA was labeled with fluorescein-12-dUTP (Thermo Scientific) using Random Primers DNA Labeling System (Invitrogen).

    Techniques: In Situ Hybridization

    The karyotype of Strigamia maritima . (A) Inverted image of a DAPI-stained mitotic metaphase prepared from embryonic material, of which the sex was unknown (left panel). The constructed karyotype (right panel) is a representative example of karyotypes prepared from at least 10 other embryos. It shows a large pair of metacentric chromosomes and seven middle to small acrocentric pairs, gradually decreasing in size. All karyotypes give qualitatively the same result and none of them show evidence for a heteromorphic sex chromosome pair. Scale bar = 10 μm. (B) Comparative genomic hybridization (CGH) on a spermatogonial mitotic metaphase prepared from the testes of a sub-adult male (left panel). Chromosomes were counterstained with DAPI (blue). Female-derived genomic probe was labelled with fluorescein-12-dUTP (green) and male-derived genomic probe with Cy3-dUTP (red). Both probes highlighted one arm of the two large metacentric chromosomes (arrows) and the centromeric heterochromatin of all chromosomes (asterisks), but did not differentiate a sex chromosome pair as demonstrated in the constructed karyotype (right panel). Scale bar = 10 μm.

    Journal: PLoS ONE

    Article Title: XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima

    doi: 10.1371/journal.pone.0150292

    Figure Lengend Snippet: The karyotype of Strigamia maritima . (A) Inverted image of a DAPI-stained mitotic metaphase prepared from embryonic material, of which the sex was unknown (left panel). The constructed karyotype (right panel) is a representative example of karyotypes prepared from at least 10 other embryos. It shows a large pair of metacentric chromosomes and seven middle to small acrocentric pairs, gradually decreasing in size. All karyotypes give qualitatively the same result and none of them show evidence for a heteromorphic sex chromosome pair. Scale bar = 10 μm. (B) Comparative genomic hybridization (CGH) on a spermatogonial mitotic metaphase prepared from the testes of a sub-adult male (left panel). Chromosomes were counterstained with DAPI (blue). Female-derived genomic probe was labelled with fluorescein-12-dUTP (green) and male-derived genomic probe with Cy3-dUTP (red). Both probes highlighted one arm of the two large metacentric chromosomes (arrows) and the centromeric heterochromatin of all chromosomes (asterisks), but did not differentiate a sex chromosome pair as demonstrated in the constructed karyotype (right panel). Scale bar = 10 μm.

    Article Snippet: The probes were labelled using a Nick Translation Kit (Abbott Molecular Inc.); male DNA with Cy3-dUTP (GE Healthcare) and female DNA with fluorescein-12-dUTP (Invitrogen).

    Techniques: Staining, Construct, Hybridization, Derivative Assay

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Journal: Scientific Reports

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    doi: 10.1038/s41598-018-34545-x

    Figure Lengend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Article Snippet: Identification of L. mollis chromosomes by GISH L. mollis genomic DNA was labeled with fluorescein-12-dUTP (Thermo Scientific) using Random Primers DNA Labeling System (Invitrogen).

    Techniques: In Situ Hybridization