fluconazole  (Selleck Chemicals)

 
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    Name:
    Fluconazole
    Description:
    Fluconazole is a fungal lanosterol 14 alpha demethylase inhibitor which thereby prevents the formation of ergosterol used in the treatment and prevention of superficial and systemic fungal infections
    Catalog Number:
    s1331
    Product Aliases:
    UK 49858
    Molecular Weight:
    306.27
    Price:
    None
    Category:
    P450 e g CYP17 inhibitor Metabolism
    Formula:
    C13H12F2N6O
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    Structured Review

    Selleck Chemicals fluconazole
    Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of <t>fluconazole,</t> econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p
    Fluconazole is a fungal lanosterol 14 alpha demethylase inhibitor which thereby prevents the formation of ergosterol used in the treatment and prevention of superficial and systemic fungal infections
    https://www.bioz.com/result/fluconazole/product/Selleck Chemicals
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2020-09
    92/100 stars

    Images

    1) Product Images from "Econazole Induces p53-Dependent Apoptosis and Decreases Metastasis Ability in Gastric Cancer Cells"

    Article Title: Econazole Induces p53-Dependent Apoptosis and Decreases Metastasis Ability in Gastric Cancer Cells

    Journal: Biomolecules & Therapeutics

    doi: 10.4062/biomolther.2019.201

    Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of fluconazole, econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p
    Figure Legend Snippet: Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of fluconazole, econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p

    Techniques Used: Multiple Displacement Amplification, Cell Culture, CCK-8 Assay, Incubation, Cell Cycle Assay, Flow Cytometry, Staining, Western Blot, Standard Deviation

    2) Product Images from "Deletion of ADA2 Increases Antifungal Drug Susceptibility and Virulence in Candida glabrata"

    Article Title: Deletion of ADA2 Increases Antifungal Drug Susceptibility and Virulence in Candida glabrata

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01924-17

    ADA2 control of antifungal drug tolerance and cell wall integrity is mediated by ERG6 . The erg6 mutants were susceptible to echinocandins, fluconazole, cell wall-perturbing agents, and the ER stress chemical tunicamycin but not amphotericin B. Cells were grown overnight in YPD at 37°C and washed twice with dH 2 O, 5-fold serially diluted, and spotted onto YPD medium containing MCF, CSF, ANF, FLC, PSC, VRC, SDS, CFW, CR, DTT, or TM at the indicated concentration. Strains were spotted onto SC medium with or without 50 ng/ml AmB to test tolerance to amphotericin B. All plates were incubated at 37°C for 30 h.
    Figure Legend Snippet: ADA2 control of antifungal drug tolerance and cell wall integrity is mediated by ERG6 . The erg6 mutants were susceptible to echinocandins, fluconazole, cell wall-perturbing agents, and the ER stress chemical tunicamycin but not amphotericin B. Cells were grown overnight in YPD at 37°C and washed twice with dH 2 O, 5-fold serially diluted, and spotted onto YPD medium containing MCF, CSF, ANF, FLC, PSC, VRC, SDS, CFW, CR, DTT, or TM at the indicated concentration. Strains were spotted onto SC medium with or without 50 ng/ml AmB to test tolerance to amphotericin B. All plates were incubated at 37°C for 30 h.

    Techniques Used: Concentration Assay, Incubation

    Related Articles

    other:

    Article Title: Deletion of ADA2 Increases Antifungal Drug Susceptibility and Virulence in Candida glabrata
    Article Snippet: Sodium dodecyl sulfate (SDS; Bioman, New Taipei City, Taiwan), calcofluor white (CFW; Fluorescent Brighter 28; Sigma), Congo red (CR; Genzyme, Cambridge, MA, USA), fluconazole (FLC; Selleckchem, Houston, TX, USA), posaconazole (PSC; Merck, Rahway, NJ, USA), voriconazole (VRC; Sigma), micafungin (MCF; Astellas Pharma Inc., Deerfield, IL, USA), caspofungin (CSF; Merck), anidulafungin (ANF; Pfizer Inc., Groton, CT, USA), dithiothreitol (DTT; BioShop, Burlington, ON, Canada), and tunicamycin (TM, Sigma) were added to the media at the concentrations indicated above and below.

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  • 92
    Selleck Chemicals fluconazole
    Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of <t>fluconazole,</t> econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p
    Fluconazole, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluconazole/product/Selleck Chemicals
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2020-09
    92/100 stars
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    92
    Selleck Chemicals zona pellucida
    Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of <t>fluconazole,</t> econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p
    Zona Pellucida, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zona pellucida/product/Selleck Chemicals
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zona pellucida - by Bioz Stars, 2020-09
    92/100 stars
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    84
    Selleck Chemicals zona pellucida 1
    Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of <t>fluconazole,</t> econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p
    Zona Pellucida 1, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zona pellucida 1/product/Selleck Chemicals
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zona pellucida 1 - by Bioz Stars, 2020-09
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    Image Search Results


    Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of fluconazole, econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p

    Journal: Biomolecules & Therapeutics

    Article Title: Econazole Induces p53-Dependent Apoptosis and Decreases Metastasis Ability in Gastric Cancer Cells

    doi: 10.4062/biomolther.2019.201

    Figure Lengend Snippet: Econazole induces the apoptosis of gastric cancer cell lines. (A) AGS, SNU1, A549, SK-hep1, SNU449, and MDA-MB-453 cells were cultured in the presence of increasing concentrations of econazole. Cell viability was determined with a CCK-8 assay after 48 h. (B) AGS cells were pre treated with the indicated concentrations of fluconazole, econazole, and miconazole for 24 h. After incubation for 15 days, control (No drug) group colonies with more than 100 colonies were counted. Econazole induced apoptosis in AGS and SNU1 gastric cancer cells. (C) Cell cycle analysis: AGS and SNU1 cells were treated with econazole at different doses for 24 h and were subjected to flow cytometry analysis. (D) Apoptosis analysis by Annexin V-PI staining. (E) Apoptosis analysis by western blotting. Data are presented as the mean ± standard deviation (n=3), * p

    Article Snippet: Econazole, fluconazole, miconazole, and Z-VAD-FMK were purchased from Selleckchem (TX, USA).

    Techniques: Multiple Displacement Amplification, Cell Culture, CCK-8 Assay, Incubation, Cell Cycle Assay, Flow Cytometry, Staining, Western Blot, Standard Deviation

    ADA2 control of antifungal drug tolerance and cell wall integrity is mediated by ERG6 . The erg6 mutants were susceptible to echinocandins, fluconazole, cell wall-perturbing agents, and the ER stress chemical tunicamycin but not amphotericin B. Cells were grown overnight in YPD at 37°C and washed twice with dH 2 O, 5-fold serially diluted, and spotted onto YPD medium containing MCF, CSF, ANF, FLC, PSC, VRC, SDS, CFW, CR, DTT, or TM at the indicated concentration. Strains were spotted onto SC medium with or without 50 ng/ml AmB to test tolerance to amphotericin B. All plates were incubated at 37°C for 30 h.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Deletion of ADA2 Increases Antifungal Drug Susceptibility and Virulence in Candida glabrata

    doi: 10.1128/AAC.01924-17

    Figure Lengend Snippet: ADA2 control of antifungal drug tolerance and cell wall integrity is mediated by ERG6 . The erg6 mutants were susceptible to echinocandins, fluconazole, cell wall-perturbing agents, and the ER stress chemical tunicamycin but not amphotericin B. Cells were grown overnight in YPD at 37°C and washed twice with dH 2 O, 5-fold serially diluted, and spotted onto YPD medium containing MCF, CSF, ANF, FLC, PSC, VRC, SDS, CFW, CR, DTT, or TM at the indicated concentration. Strains were spotted onto SC medium with or without 50 ng/ml AmB to test tolerance to amphotericin B. All plates were incubated at 37°C for 30 h.

    Article Snippet: Sodium dodecyl sulfate (SDS; Bioman, New Taipei City, Taiwan), calcofluor white (CFW; Fluorescent Brighter 28; Sigma), Congo red (CR; Genzyme, Cambridge, MA, USA), fluconazole (FLC; Selleckchem, Houston, TX, USA), posaconazole (PSC; Merck, Rahway, NJ, USA), voriconazole (VRC; Sigma), micafungin (MCF; Astellas Pharma Inc., Deerfield, IL, USA), caspofungin (CSF; Merck), anidulafungin (ANF; Pfizer Inc., Groton, CT, USA), dithiothreitol (DTT; BioShop, Burlington, ON, Canada), and tunicamycin (TM, Sigma) were added to the media at the concentrations indicated above and below.

    Techniques: Concentration Assay, Incubation