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IgG isotypes production against rPb27 by infected and immunized mice. Antibody response against rPb27 in mice infected and immunized with this recombinant protein associated or not with <t>fluconazole</t> chemotherapy was determined by ELISA assay after 40 (A) and 90 (B) days of treatment. Control, mice without any intervention. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. Bars represent the means and standard deviations of optical density (O.D.) at 1∶400 serum dilution in each experimental group (n = 3). * significant (p
Fluconazole, supplied by Mantena Laboratories Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0017885

IgG isotypes production against rPb27 by infected and immunized mice. Antibody response against rPb27 in mice infected and immunized with this recombinant protein associated or not with fluconazole chemotherapy was determined by ELISA assay after 40 (A) and 90 (B) days of treatment. Control, mice without any intervention. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. Bars represent the means and standard deviations of optical density (O.D.) at 1∶400 serum dilution in each experimental group (n = 3). * significant (p
Figure Legend Snippet: IgG isotypes production against rPb27 by infected and immunized mice. Antibody response against rPb27 in mice infected and immunized with this recombinant protein associated or not with fluconazole chemotherapy was determined by ELISA assay after 40 (A) and 90 (B) days of treatment. Control, mice without any intervention. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. Bars represent the means and standard deviations of optical density (O.D.) at 1∶400 serum dilution in each experimental group (n = 3). * significant (p

Techniques Used: Infection, Mouse Assay, Recombinant, Enzyme-linked Immunosorbent Assay

Representative histopathology of lungs, livers and spleens from infected mice, after 40 days of treatment. BALB/c mice were euthanized 40 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 427.3 µm, while in each liver and spleen photos, the scale bar represents 56.9 µm.
Figure Legend Snippet: Representative histopathology of lungs, livers and spleens from infected mice, after 40 days of treatment. BALB/c mice were euthanized 40 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 427.3 µm, while in each liver and spleen photos, the scale bar represents 56.9 µm.

Techniques Used: Histopathology, Infection, Mouse Assay, Staining

Fungal recovery in lung, spleen and liver of infected mice. The CFUs were estimated 40 (A) and 90 days (B) post treatment in organs from mice infected intratracheally with 3×10 5 P. brasiliensis yeast cells and subjected to fluconazole treatment combined or not with rPb27 immunization. Control mice were only infected with P. brasiliensis (Infected), adjuvant mice were inoculated with C. parvum -Al(OH) 3 with fluconazole treatment (Adjuvant/T) or not (Adjuvant), and rPb27 mice were immunized with recombinant protein combined to fluconazole treatment (rPb27/T) or not (rPb27). All groups of mice were infected with the same number of yeast cells. Bars represent the Log 10 (UFC/g) means and standard deviations from organs of 3 to 5 animals in each group. * significant (p
Figure Legend Snippet: Fungal recovery in lung, spleen and liver of infected mice. The CFUs were estimated 40 (A) and 90 days (B) post treatment in organs from mice infected intratracheally with 3×10 5 P. brasiliensis yeast cells and subjected to fluconazole treatment combined or not with rPb27 immunization. Control mice were only infected with P. brasiliensis (Infected), adjuvant mice were inoculated with C. parvum -Al(OH) 3 with fluconazole treatment (Adjuvant/T) or not (Adjuvant), and rPb27 mice were immunized with recombinant protein combined to fluconazole treatment (rPb27/T) or not (rPb27). All groups of mice were infected with the same number of yeast cells. Bars represent the Log 10 (UFC/g) means and standard deviations from organs of 3 to 5 animals in each group. * significant (p

Techniques Used: Infection, Mouse Assay, Recombinant

Representative granulome histopathology of lungs from infected mice after 40 and 90 days of infection. BALB/c mice were euthanized 40 and 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27/T, group infected and posteriorly immunized with rPb27 and treated with fluconazole. In each photo, the scale bar represents 25.9 µm.
Figure Legend Snippet: Representative granulome histopathology of lungs from infected mice after 40 and 90 days of infection. BALB/c mice were euthanized 40 and 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27/T, group infected and posteriorly immunized with rPb27 and treated with fluconazole. In each photo, the scale bar represents 25.9 µm.

Techniques Used: Histopathology, Infection, Mouse Assay, Staining

Representative histopathology of lungs, livers and spleens from infected mice, after 90 days of treatment. BALB/c mice were euthanized 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 416.6 µm, while in each liver and spleen photos, the scale bar represents 55.6 µm.
Figure Legend Snippet: Representative histopathology of lungs, livers and spleens from infected mice, after 90 days of treatment. BALB/c mice were euthanized 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 416.6 µm, while in each liver and spleen photos, the scale bar represents 55.6 µm.

Techniques Used: Histopathology, Infection, Mouse Assay, Staining

Related Articles

Infection:

Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis
Article Snippet: .. Chemotherapy of infected mice After 30 days of infection animals were treated for 30 days during which groups of mice received doses every 24 h of fluconazole 10 mg/Kg (Mantena laboratories limited). ..

Mouse Assay:

Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis
Article Snippet: .. Chemotherapy of infected mice After 30 days of infection animals were treated for 30 days during which groups of mice received doses every 24 h of fluconazole 10 mg/Kg (Mantena laboratories limited). ..

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    Mantena Laboratories Ltd fluconazole
    IgG isotypes production against rPb27 by infected and immunized mice. Antibody response against rPb27 in mice infected and immunized with this recombinant protein associated or not with <t>fluconazole</t> chemotherapy was determined by ELISA assay after 40 (A) and 90 (B) days of treatment. Control, mice without any intervention. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. Bars represent the means and standard deviations of optical density (O.D.) at 1∶400 serum dilution in each experimental group (n = 3). * significant (p
    Fluconazole, supplied by Mantena Laboratories Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluconazole/product/Mantena Laboratories Ltd
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2020-09
    92/100 stars
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    IgG isotypes production against rPb27 by infected and immunized mice. Antibody response against rPb27 in mice infected and immunized with this recombinant protein associated or not with fluconazole chemotherapy was determined by ELISA assay after 40 (A) and 90 (B) days of treatment. Control, mice without any intervention. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. Bars represent the means and standard deviations of optical density (O.D.) at 1∶400 serum dilution in each experimental group (n = 3). * significant (p

    Journal: PLoS ONE

    Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis

    doi: 10.1371/journal.pone.0017885

    Figure Lengend Snippet: IgG isotypes production against rPb27 by infected and immunized mice. Antibody response against rPb27 in mice infected and immunized with this recombinant protein associated or not with fluconazole chemotherapy was determined by ELISA assay after 40 (A) and 90 (B) days of treatment. Control, mice without any intervention. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. Bars represent the means and standard deviations of optical density (O.D.) at 1∶400 serum dilution in each experimental group (n = 3). * significant (p

    Article Snippet: Chemotherapy of infected mice After 30 days of infection animals were treated for 30 days during which groups of mice received doses every 24 h of fluconazole 10 mg/Kg (Mantena laboratories limited).

    Techniques: Infection, Mouse Assay, Recombinant, Enzyme-linked Immunosorbent Assay

    Representative histopathology of lungs, livers and spleens from infected mice, after 40 days of treatment. BALB/c mice were euthanized 40 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 427.3 µm, while in each liver and spleen photos, the scale bar represents 56.9 µm.

    Journal: PLoS ONE

    Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis

    doi: 10.1371/journal.pone.0017885

    Figure Lengend Snippet: Representative histopathology of lungs, livers and spleens from infected mice, after 40 days of treatment. BALB/c mice were euthanized 40 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 427.3 µm, while in each liver and spleen photos, the scale bar represents 56.9 µm.

    Article Snippet: Chemotherapy of infected mice After 30 days of infection animals were treated for 30 days during which groups of mice received doses every 24 h of fluconazole 10 mg/Kg (Mantena laboratories limited).

    Techniques: Histopathology, Infection, Mouse Assay, Staining

    Fungal recovery in lung, spleen and liver of infected mice. The CFUs were estimated 40 (A) and 90 days (B) post treatment in organs from mice infected intratracheally with 3×10 5 P. brasiliensis yeast cells and subjected to fluconazole treatment combined or not with rPb27 immunization. Control mice were only infected with P. brasiliensis (Infected), adjuvant mice were inoculated with C. parvum -Al(OH) 3 with fluconazole treatment (Adjuvant/T) or not (Adjuvant), and rPb27 mice were immunized with recombinant protein combined to fluconazole treatment (rPb27/T) or not (rPb27). All groups of mice were infected with the same number of yeast cells. Bars represent the Log 10 (UFC/g) means and standard deviations from organs of 3 to 5 animals in each group. * significant (p

    Journal: PLoS ONE

    Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis

    doi: 10.1371/journal.pone.0017885

    Figure Lengend Snippet: Fungal recovery in lung, spleen and liver of infected mice. The CFUs were estimated 40 (A) and 90 days (B) post treatment in organs from mice infected intratracheally with 3×10 5 P. brasiliensis yeast cells and subjected to fluconazole treatment combined or not with rPb27 immunization. Control mice were only infected with P. brasiliensis (Infected), adjuvant mice were inoculated with C. parvum -Al(OH) 3 with fluconazole treatment (Adjuvant/T) or not (Adjuvant), and rPb27 mice were immunized with recombinant protein combined to fluconazole treatment (rPb27/T) or not (rPb27). All groups of mice were infected with the same number of yeast cells. Bars represent the Log 10 (UFC/g) means and standard deviations from organs of 3 to 5 animals in each group. * significant (p

    Article Snippet: Chemotherapy of infected mice After 30 days of infection animals were treated for 30 days during which groups of mice received doses every 24 h of fluconazole 10 mg/Kg (Mantena laboratories limited).

    Techniques: Infection, Mouse Assay, Recombinant

    Representative granulome histopathology of lungs from infected mice after 40 and 90 days of infection. BALB/c mice were euthanized 40 and 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27/T, group infected and posteriorly immunized with rPb27 and treated with fluconazole. In each photo, the scale bar represents 25.9 µm.

    Journal: PLoS ONE

    Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis

    doi: 10.1371/journal.pone.0017885

    Figure Lengend Snippet: Representative granulome histopathology of lungs from infected mice after 40 and 90 days of infection. BALB/c mice were euthanized 40 and 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27/T, group infected and posteriorly immunized with rPb27 and treated with fluconazole. In each photo, the scale bar represents 25.9 µm.

    Article Snippet: Chemotherapy of infected mice After 30 days of infection animals were treated for 30 days during which groups of mice received doses every 24 h of fluconazole 10 mg/Kg (Mantena laboratories limited).

    Techniques: Histopathology, Infection, Mouse Assay, Staining

    Representative histopathology of lungs, livers and spleens from infected mice, after 90 days of treatment. BALB/c mice were euthanized 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 416.6 µm, while in each liver and spleen photos, the scale bar represents 55.6 µm.

    Journal: PLoS ONE

    Article Title: Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis

    doi: 10.1371/journal.pone.0017885

    Figure Lengend Snippet: Representative histopathology of lungs, livers and spleens from infected mice, after 90 days of treatment. BALB/c mice were euthanized 90 days after treatment. The lungs, spleens, and livers were excised, fixed in 10% buffered formalin, and embedded in paraffin for sectioning. The sections were stained with hematoxylin–eosin and examined microscopically. Infected, group only infected with P. brasiliensis . Infected/T, same as Infected, but treated with fluconazole. rPb27, group infected and posteriorly immunized with rPb27. rPb27/T, same as rPb27, but treated with fluconazole. In each lung photos, the scale bar represents 416.6 µm, while in each liver and spleen photos, the scale bar represents 55.6 µm.

    Article Snippet: Chemotherapy of infected mice After 30 days of infection animals were treated for 30 days during which groups of mice received doses every 24 h of fluconazole 10 mg/Kg (Mantena laboratories limited).

    Techniques: Histopathology, Infection, Mouse Assay, Staining