Structured Review

Enzo Biochem fluconazole
Dectin-1 is required for CD4 + T-cell survival. ( a ) The frequency of apoptotic CD4 + T-cells was measured by Annexin-V staining in mLN cell suspensions at the indicated time points post infection (WT n =9, KO n =9 at each time point). ( b ) CD8 + T-cell apoptosis and necrosis was measured by Annexin-V/7-AAD staining in mLN cell suspensions at 72 h post infection ( n =6). ( c ) TUNEL staining performed on paraformaldehyde-fixed mLNs from infected WT and KO mice 72 h post infection; photo taken at 5 × magnification, insert at 40 × magnification. Blue=DAPI, green=apoptotic cells. ( d ) The total number of recoverable cells from the mLN (WT n =9, KO n =9 at each time point). ( e ) H E staining of mLNs at 72 h post infection compared with naive controls (scale bars are 500 μm). ( f ) The total number of visible ( > 1 mm 3 ) Peyer's patches (infected n =9, naive n =8) in WT and Dectin-1 −/− mice at 72 h post infection. ( g ) The total number of recoverable cells from the mLN of infected WT ( n =10) and KO ( n =9) mice at 3 days post infection while maintained on cholestyramine diets, as described in Figure 1 . ( h ) Schematic of the colitis model where co-housed WT and Dectin-1 KO animals were maintained on water supplemented with penicillin, streptomycin, and <t>fluconazole</t> for 4 days, and then switched to water containing 3.5% DSS and C. tropicalis for 5 days. The number of ( i ) Peyer's patches and ( j ) number of DCs (CD11c + MHCII + ) isolated from mLNs from WT ( n =20) and Dectin-1 KO ( n =16) mice at 5 days post DSS exposure was compared with Candida only WT animals (control, n =6). Bar charts show pooled data (2–5 experiments); overlaid dot plots show one representative experiment. * P
Fluconazole, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 10 article reviews
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1) Product Images from "CD4+ T-cell survival in the GI tract requires dectin-1 during fungal infection"

Article Title: CD4+ T-cell survival in the GI tract requires dectin-1 during fungal infection

Journal: Mucosal Immunology

doi: 10.1038/mi.2015.79

Dectin-1 is required for CD4 + T-cell survival. ( a ) The frequency of apoptotic CD4 + T-cells was measured by Annexin-V staining in mLN cell suspensions at the indicated time points post infection (WT n =9, KO n =9 at each time point). ( b ) CD8 + T-cell apoptosis and necrosis was measured by Annexin-V/7-AAD staining in mLN cell suspensions at 72 h post infection ( n =6). ( c ) TUNEL staining performed on paraformaldehyde-fixed mLNs from infected WT and KO mice 72 h post infection; photo taken at 5 × magnification, insert at 40 × magnification. Blue=DAPI, green=apoptotic cells. ( d ) The total number of recoverable cells from the mLN (WT n =9, KO n =9 at each time point). ( e ) H E staining of mLNs at 72 h post infection compared with naive controls (scale bars are 500 μm). ( f ) The total number of visible ( > 1 mm 3 ) Peyer's patches (infected n =9, naive n =8) in WT and Dectin-1 −/− mice at 72 h post infection. ( g ) The total number of recoverable cells from the mLN of infected WT ( n =10) and KO ( n =9) mice at 3 days post infection while maintained on cholestyramine diets, as described in Figure 1 . ( h ) Schematic of the colitis model where co-housed WT and Dectin-1 KO animals were maintained on water supplemented with penicillin, streptomycin, and fluconazole for 4 days, and then switched to water containing 3.5% DSS and C. tropicalis for 5 days. The number of ( i ) Peyer's patches and ( j ) number of DCs (CD11c + MHCII + ) isolated from mLNs from WT ( n =20) and Dectin-1 KO ( n =16) mice at 5 days post DSS exposure was compared with Candida only WT animals (control, n =6). Bar charts show pooled data (2–5 experiments); overlaid dot plots show one representative experiment. * P
Figure Legend Snippet: Dectin-1 is required for CD4 + T-cell survival. ( a ) The frequency of apoptotic CD4 + T-cells was measured by Annexin-V staining in mLN cell suspensions at the indicated time points post infection (WT n =9, KO n =9 at each time point). ( b ) CD8 + T-cell apoptosis and necrosis was measured by Annexin-V/7-AAD staining in mLN cell suspensions at 72 h post infection ( n =6). ( c ) TUNEL staining performed on paraformaldehyde-fixed mLNs from infected WT and KO mice 72 h post infection; photo taken at 5 × magnification, insert at 40 × magnification. Blue=DAPI, green=apoptotic cells. ( d ) The total number of recoverable cells from the mLN (WT n =9, KO n =9 at each time point). ( e ) H E staining of mLNs at 72 h post infection compared with naive controls (scale bars are 500 μm). ( f ) The total number of visible ( > 1 mm 3 ) Peyer's patches (infected n =9, naive n =8) in WT and Dectin-1 −/− mice at 72 h post infection. ( g ) The total number of recoverable cells from the mLN of infected WT ( n =10) and KO ( n =9) mice at 3 days post infection while maintained on cholestyramine diets, as described in Figure 1 . ( h ) Schematic of the colitis model where co-housed WT and Dectin-1 KO animals were maintained on water supplemented with penicillin, streptomycin, and fluconazole for 4 days, and then switched to water containing 3.5% DSS and C. tropicalis for 5 days. The number of ( i ) Peyer's patches and ( j ) number of DCs (CD11c + MHCII + ) isolated from mLNs from WT ( n =20) and Dectin-1 KO ( n =16) mice at 5 days post DSS exposure was compared with Candida only WT animals (control, n =6). Bar charts show pooled data (2–5 experiments); overlaid dot plots show one representative experiment. * P

Techniques Used: Staining, Infection, TUNEL Assay, Mouse Assay, Isolation

2) Product Images from "Interaction of human drug-metabolizing CYP3A4 with small inhibitory molecules"

Article Title: Interaction of human drug-metabolizing CYP3A4 with small inhibitory molecules

Journal: Biochemistry

doi: 10.1021/acs.biochem.8b01221

Crystal structure of the CYP3A4-PMSF complex. A , View at the active site of PMSF-bound CYP3A4. The sulfofluoride moiety of PMSF forms strong H-bonds (red dashed lines) with the Ser119 and Arg212 side groups. The distal water ligand and the neighboring solvent molecule are depicted as red spheres. Simulated annealing and polder omit maps are shown as green and gray mesh contoured at 3σ and 5σ levels, respectively. B , PMSA fits equally well into the PMSF binding site, and PMSF-to-PMSA substitution does not affect the refinement statistics. C , Comparison of the binding modes of metyrapone, fluconazole and PMSF (shown in yellow, green and magenta, respectively).
Figure Legend Snippet: Crystal structure of the CYP3A4-PMSF complex. A , View at the active site of PMSF-bound CYP3A4. The sulfofluoride moiety of PMSF forms strong H-bonds (red dashed lines) with the Ser119 and Arg212 side groups. The distal water ligand and the neighboring solvent molecule are depicted as red spheres. Simulated annealing and polder omit maps are shown as green and gray mesh contoured at 3σ and 5σ levels, respectively. B , PMSA fits equally well into the PMSF binding site, and PMSF-to-PMSA substitution does not affect the refinement statistics. C , Comparison of the binding modes of metyrapone, fluconazole and PMSF (shown in yellow, green and magenta, respectively).

Techniques Used: Binding Assay

Related Articles

other:

Article Title: Interaction of human drug-metabolizing CYP3A4 with small inhibitory molecules
Article Snippet: Metyrapone, fluconazole, PMSF and PMSA were purchased from Enzo Life Sciences, TCI America, Gold Biotechnology and Sigma-Aldrich, respectively.

Mouse Assay:

Article Title: Dectin-1 Is Not Required for Controlling Candida albicans Colonization of the Gastrointestinal Tract
Article Snippet: .. To reduce the commensal bacterial and endogenous fungal microbiota, mice were provided with sterile water containing 2 mg/ml streptomycin (Invitrogen), 2,000 U/ml penicillin (Invitrogen), and 0.25 mg/ml fluconazole (Enzo) for 3 days and then switched to water containing streptomycin and penicillin for a further 24 h. Mice were then provided with sterile water containing 1 × 107 CFU/ml C. albicans , 2 mg/ml streptomycin, and 2,000 U/ml penicillin for 5 days. .. After C. albicans exposure, mice were maintained on sterile water containing 2 mg/ml streptomycin, 2,000 U/ml penicillin, and 0.2 mg/ml gentamicin (Invitrogen).

Article Title: Candida albicans colonization and dissemination from the murine gastrointestinal tract: the influence of morphology and Th17 immunity
Article Snippet: .. To reduce commensal flora, mice were provided with sterile antibiotic water containing 2 mg ml−1 of streptomycin (Invitrogen), 2000 U ml−1 of penicillin (Invitrogen), 0.25 mg ml−1 of fluconazole (Enzo) for 3 days and then switched to water containing the same concentrations of streptomycin and penicillin for a further 24 h. Mice were then provided with sterile water containing 1 × 107 CFU ml−1 of C. albicans , 2 mg ml−1 of streptomycin and 2000 U ml−1 of penicillin for 5 days. .. After C. albicans exposure, mice were maintained on sterile water containing 2 mg ml−1 of streptomycin, 2000 U ml−1 of penicillin and 0.2 mg ml−1 of gentamicin (Invitrogen).

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    Enzo Biochem fluconazole
    Dectin-1 is required for CD4 + T-cell survival. ( a ) The frequency of apoptotic CD4 + T-cells was measured by Annexin-V staining in mLN cell suspensions at the indicated time points post infection (WT n =9, KO n =9 at each time point). ( b ) CD8 + T-cell apoptosis and necrosis was measured by Annexin-V/7-AAD staining in mLN cell suspensions at 72 h post infection ( n =6). ( c ) TUNEL staining performed on paraformaldehyde-fixed mLNs from infected WT and KO mice 72 h post infection; photo taken at 5 × magnification, insert at 40 × magnification. Blue=DAPI, green=apoptotic cells. ( d ) The total number of recoverable cells from the mLN (WT n =9, KO n =9 at each time point). ( e ) H E staining of mLNs at 72 h post infection compared with naive controls (scale bars are 500 μm). ( f ) The total number of visible ( > 1 mm 3 ) Peyer's patches (infected n =9, naive n =8) in WT and Dectin-1 −/− mice at 72 h post infection. ( g ) The total number of recoverable cells from the mLN of infected WT ( n =10) and KO ( n =9) mice at 3 days post infection while maintained on cholestyramine diets, as described in Figure 1 . ( h ) Schematic of the colitis model where co-housed WT and Dectin-1 KO animals were maintained on water supplemented with penicillin, streptomycin, and <t>fluconazole</t> for 4 days, and then switched to water containing 3.5% DSS and C. tropicalis for 5 days. The number of ( i ) Peyer's patches and ( j ) number of DCs (CD11c + MHCII + ) isolated from mLNs from WT ( n =20) and Dectin-1 KO ( n =16) mice at 5 days post DSS exposure was compared with Candida only WT animals (control, n =6). Bar charts show pooled data (2–5 experiments); overlaid dot plots show one representative experiment. * P
    Fluconazole, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluconazole/product/Enzo Biochem
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    fluconazole - by Bioz Stars, 2020-09
    92/100 stars
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    Dectin-1 is required for CD4 + T-cell survival. ( a ) The frequency of apoptotic CD4 + T-cells was measured by Annexin-V staining in mLN cell suspensions at the indicated time points post infection (WT n =9, KO n =9 at each time point). ( b ) CD8 + T-cell apoptosis and necrosis was measured by Annexin-V/7-AAD staining in mLN cell suspensions at 72 h post infection ( n =6). ( c ) TUNEL staining performed on paraformaldehyde-fixed mLNs from infected WT and KO mice 72 h post infection; photo taken at 5 × magnification, insert at 40 × magnification. Blue=DAPI, green=apoptotic cells. ( d ) The total number of recoverable cells from the mLN (WT n =9, KO n =9 at each time point). ( e ) H E staining of mLNs at 72 h post infection compared with naive controls (scale bars are 500 μm). ( f ) The total number of visible ( > 1 mm 3 ) Peyer's patches (infected n =9, naive n =8) in WT and Dectin-1 −/− mice at 72 h post infection. ( g ) The total number of recoverable cells from the mLN of infected WT ( n =10) and KO ( n =9) mice at 3 days post infection while maintained on cholestyramine diets, as described in Figure 1 . ( h ) Schematic of the colitis model where co-housed WT and Dectin-1 KO animals were maintained on water supplemented with penicillin, streptomycin, and fluconazole for 4 days, and then switched to water containing 3.5% DSS and C. tropicalis for 5 days. The number of ( i ) Peyer's patches and ( j ) number of DCs (CD11c + MHCII + ) isolated from mLNs from WT ( n =20) and Dectin-1 KO ( n =16) mice at 5 days post DSS exposure was compared with Candida only WT animals (control, n =6). Bar charts show pooled data (2–5 experiments); overlaid dot plots show one representative experiment. * P

    Journal: Mucosal Immunology

    Article Title: CD4+ T-cell survival in the GI tract requires dectin-1 during fungal infection

    doi: 10.1038/mi.2015.79

    Figure Lengend Snippet: Dectin-1 is required for CD4 + T-cell survival. ( a ) The frequency of apoptotic CD4 + T-cells was measured by Annexin-V staining in mLN cell suspensions at the indicated time points post infection (WT n =9, KO n =9 at each time point). ( b ) CD8 + T-cell apoptosis and necrosis was measured by Annexin-V/7-AAD staining in mLN cell suspensions at 72 h post infection ( n =6). ( c ) TUNEL staining performed on paraformaldehyde-fixed mLNs from infected WT and KO mice 72 h post infection; photo taken at 5 × magnification, insert at 40 × magnification. Blue=DAPI, green=apoptotic cells. ( d ) The total number of recoverable cells from the mLN (WT n =9, KO n =9 at each time point). ( e ) H E staining of mLNs at 72 h post infection compared with naive controls (scale bars are 500 μm). ( f ) The total number of visible ( > 1 mm 3 ) Peyer's patches (infected n =9, naive n =8) in WT and Dectin-1 −/− mice at 72 h post infection. ( g ) The total number of recoverable cells from the mLN of infected WT ( n =10) and KO ( n =9) mice at 3 days post infection while maintained on cholestyramine diets, as described in Figure 1 . ( h ) Schematic of the colitis model where co-housed WT and Dectin-1 KO animals were maintained on water supplemented with penicillin, streptomycin, and fluconazole for 4 days, and then switched to water containing 3.5% DSS and C. tropicalis for 5 days. The number of ( i ) Peyer's patches and ( j ) number of DCs (CD11c + MHCII + ) isolated from mLNs from WT ( n =20) and Dectin-1 KO ( n =16) mice at 5 days post DSS exposure was compared with Candida only WT animals (control, n =6). Bar charts show pooled data (2–5 experiments); overlaid dot plots show one representative experiment. * P

    Article Snippet: Colitis model. Co-housed WT and Dectin-1−/− animals were maintained on sterile water supplemented with 2 mg ml−1 streptomycin, 2,000 U ml−1 penicillin (both Invitrogen) and 0.25 mg ml−1 fluconazole (Enzo, Exeter, UK) for 4 days prior to start of experiments.

    Techniques: Staining, Infection, TUNEL Assay, Mouse Assay, Isolation