cf chp  (Echelon Biosciences)


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    Echelon Biosciences cf chp
    ( A ) Representative images of <t>CF-CHP</t> (green indicative of denatured collagen) <t>stained</t> <t>cryosections</t> from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.
    Cf Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cf chp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cf chp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Detergent-Free Decellularization Preserves the Structural and Biological Integrity of Murine Tendon"

    Article Title: Detergent-Free Decellularization Preserves the Structural and Biological Integrity of Murine Tendon

    Journal: bioRxiv

    doi: 10.1101/2022.07.11.499615

    ( A ) Representative images of CF-CHP (green indicative of denatured collagen) stained cryosections from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.
    Figure Legend Snippet: ( A ) Representative images of CF-CHP (green indicative of denatured collagen) stained cryosections from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.

    Techniques Used: Staining, Standard Deviation

    cf chp  (Echelon Biosciences)


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  • 94

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    Echelon Biosciences cf chp
    ( A ) Representative images of <t>CF-CHP</t> (green indicative of denatured collagen) <t>stained</t> <t>cryosections</t> from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.
    Cf Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cf chp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cf chp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Detergent-Free Decellularization Preserves the Structural and Biological Integrity of Murine Tendon"

    Article Title: Detergent-Free Decellularization Preserves the Structural and Biological Integrity of Murine Tendon

    Journal: bioRxiv

    doi: 10.1101/2022.07.11.499615

    ( A ) Representative images of CF-CHP (green indicative of denatured collagen) stained cryosections from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.
    Figure Legend Snippet: ( A ) Representative images of CF-CHP (green indicative of denatured collagen) stained cryosections from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.

    Techniques Used: Staining, Standard Deviation

    bv chp  (Echelon Biosciences)


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    Echelon Biosciences bv chp
    Randomized (published) studies in frontline treatment of PTCLs.
    Bv Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bv chp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bv chp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Peripheral T-Cell Lymphomas: Therapeutic Approaches"

    Article Title: Peripheral T-Cell Lymphomas: Therapeutic Approaches

    Journal: Cancers

    doi: 10.3390/cancers14092332

    Randomized (published) studies in frontline treatment of PTCLs.
    Figure Legend Snippet: Randomized (published) studies in frontline treatment of PTCLs.

    Techniques Used: Modification

    Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).
    Figure Legend Snippet: Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).

    Techniques Used:

    bv chp  (Echelon Biosciences)


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  • 94

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    Echelon Biosciences bv chp
    Randomized (published) studies in frontline treatment of PTCLs.
    Bv Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bv chp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bv chp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Peripheral T-Cell Lymphomas: Therapeutic Approaches"

    Article Title: Peripheral T-Cell Lymphomas: Therapeutic Approaches

    Journal: Cancers

    doi: 10.3390/cancers14092332

    Randomized (published) studies in frontline treatment of PTCLs.
    Figure Legend Snippet: Randomized (published) studies in frontline treatment of PTCLs.

    Techniques Used: Modification

    Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).
    Figure Legend Snippet: Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).

    Techniques Used:

    bv chp  (Echelon Biosciences)


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  • 94

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    Echelon Biosciences bv chp
    Randomized (published) studies in frontline treatment of PTCLs.
    Bv Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bv chp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bv chp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Peripheral T-Cell Lymphomas: Therapeutic Approaches"

    Article Title: Peripheral T-Cell Lymphomas: Therapeutic Approaches

    Journal: Cancers

    doi: 10.3390/cancers14092332

    Randomized (published) studies in frontline treatment of PTCLs.
    Figure Legend Snippet: Randomized (published) studies in frontline treatment of PTCLs.

    Techniques Used: Modification

    Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).
    Figure Legend Snippet: Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).

    Techniques Used:

    fluorescein isothiocyanate  (Echelon Biosciences)


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  • 94

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    Echelon Biosciences fluorescein isothiocyanate
    Fluorescein Isothiocyanate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein isothiocyanate/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
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    fluorescein isothiocyanate - by Bioz Stars, 2023-01
    94/100 stars

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    collagen  (Echelon Biosciences)


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    Echelon Biosciences collagen
    Collagen, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagen/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagen - by Bioz Stars, 2023-01
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    fluorescein conjugated collagen hybridizing peptide chp  (Echelon Biosciences)


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    Echelon Biosciences fluorescein conjugated collagen hybridizing peptide chp
    Mosquito feeding promotes the degradation of the midgut basal lamina. Using a <t>fluorescein-labeled</t> <t>collagen</t> hybridizing peptide <t>(CHP)</t> to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).
    Fluorescein Conjugated Collagen Hybridizing Peptide Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein conjugated collagen hybridizing peptide chp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein conjugated collagen hybridizing peptide chp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Additional Feeding Reveals Differences in Immune Recognition and Growth of Plasmodium Parasites in the Mosquito Host"

    Article Title: Additional Feeding Reveals Differences in Immune Recognition and Growth of Plasmodium Parasites in the Mosquito Host

    Journal: mSphere

    doi: 10.1128/mSphere.00136-21

    Mosquito feeding promotes the degradation of the midgut basal lamina. Using a fluorescein-labeled collagen hybridizing peptide (CHP) to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).
    Figure Legend Snippet: Mosquito feeding promotes the degradation of the midgut basal lamina. Using a fluorescein-labeled collagen hybridizing peptide (CHP) to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).

    Techniques Used: Labeling, Positive Control, Fluorescence, Binding Assay, Microscopy, Software

    fluorescein pip 2 hg  (Echelon Biosciences)


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    Echelon Biosciences fluorescein pip 2 hg
    Fluorescein Pip 2 Hg, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein pip 2 hg/product/Echelon Biosciences
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    94/100 stars

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    echelon 2 bv chp arm  (Echelon Biosciences)


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    Echelon Biosciences echelon 2 bv chp arm
    Echelon 2 Bv Chp Arm, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/echelon 2 bv chp arm/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
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    fluorescein conjugated collagen hybridizing peptide chp  (Echelon Biosciences)


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    Echelon Biosciences fluorescein conjugated collagen hybridizing peptide chp
    Fluorescein Conjugated Collagen Hybridizing Peptide Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein conjugated collagen hybridizing peptide chp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
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    fluorescein conjugated collagen hybridizing peptide chp - by Bioz Stars, 2023-01
    94/100 stars

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    Echelon Biosciences cf chp
    ( A ) Representative images of <t>CF-CHP</t> (green indicative of denatured collagen) <t>stained</t> <t>cryosections</t> from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.
    Cf Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cf chp/product/Echelon Biosciences
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    Echelon Biosciences bv chp
    Randomized (published) studies in frontline treatment of PTCLs.
    Bv Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences fluorescein isothiocyanate
    Randomized (published) studies in frontline treatment of PTCLs.
    Fluorescein Isothiocyanate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Randomized (published) studies in frontline treatment of PTCLs.
    Collagen, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagen/product/Echelon Biosciences
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    Echelon Biosciences fluorescein conjugated collagen hybridizing peptide chp
    Mosquito feeding promotes the degradation of the midgut basal lamina. Using a <t>fluorescein-labeled</t> <t>collagen</t> hybridizing peptide <t>(CHP)</t> to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).
    Fluorescein Conjugated Collagen Hybridizing Peptide Chp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences fluorescein pip 2 hg
    Mosquito feeding promotes the degradation of the midgut basal lamina. Using a <t>fluorescein-labeled</t> <t>collagen</t> hybridizing peptide <t>(CHP)</t> to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).
    Fluorescein Pip 2 Hg, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mosquito feeding promotes the degradation of the midgut basal lamina. Using a <t>fluorescein-labeled</t> <t>collagen</t> hybridizing peptide <t>(CHP)</t> to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).
    Echelon 2 Bv Chp Arm, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Representative images of CF-CHP (green indicative of denatured collagen) stained cryosections from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Detergent-Free Decellularization Preserves the Structural and Biological Integrity of Murine Tendon

    doi: 10.1101/2022.07.11.499615

    Figure Lengend Snippet: ( A ) Representative images of CF-CHP (green indicative of denatured collagen) stained cryosections from B6 naïve patellar tendon for non-decellularized, decellularized, and heat denatured conditions. Dashed white lines indicate the tissue outline. Scale bar, 200 µm. N = 6 samples per group. ( B ) Quantification of CF-CHP integrated density illustrated minimal collagen denaturation in non-decellularized and decellularized tendons. Data is presented as mean ± standard deviation. **** P < 0.0001.

    Article Snippet: To determine the extent of collagen denaturation after detergent-free decellularization, non-decellularized and decellularized cryosections were stained with 20 µM of carboxyfluorescein-labeled collagen hybridizing peptide – (CF-CHP; Echelon Biosciences, #FLU300) in 1X PBS as per the manufacturer’s instructions.

    Techniques: Staining, Standard Deviation

    Randomized (published) studies in frontline treatment of PTCLs.

    Journal: Cancers

    Article Title: Peripheral T-Cell Lymphomas: Therapeutic Approaches

    doi: 10.3390/cancers14092332

    Figure Lengend Snippet: Randomized (published) studies in frontline treatment of PTCLs.

    Article Snippet: ECHELON-2 , BV-CHP , Modified PFS by independent central review , BV-CHP (226) CHOP (226) , 83%/68% 72%/56% , 3-year PFS 57% (BV-CHP) vs. 44% (CHOP) 3-year OS 77% (BV-CHP) vs. 69% (CHOP) , Horwitz S, 2019 [ ] .

    Techniques: Modification

    Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).

    Journal: Cancers

    Article Title: Peripheral T-Cell Lymphomas: Therapeutic Approaches

    doi: 10.3390/cancers14092332

    Figure Lengend Snippet: Phase 1 and 2 studies combining CHOP/CHOP-like with novel agents (published studies).

    Article Snippet: ECHELON-2 , BV-CHP , Modified PFS by independent central review , BV-CHP (226) CHOP (226) , 83%/68% 72%/56% , 3-year PFS 57% (BV-CHP) vs. 44% (CHOP) 3-year OS 77% (BV-CHP) vs. 69% (CHOP) , Horwitz S, 2019 [ ] .

    Techniques:

    Mosquito feeding promotes the degradation of the midgut basal lamina. Using a fluorescein-labeled collagen hybridizing peptide (CHP) to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).

    Journal: mSphere

    Article Title: Additional Feeding Reveals Differences in Immune Recognition and Growth of Plasmodium Parasites in the Mosquito Host

    doi: 10.1128/mSphere.00136-21

    Figure Lengend Snippet: Mosquito feeding promotes the degradation of the midgut basal lamina. Using a fluorescein-labeled collagen hybridizing peptide (CHP) to detect degraded collagen, midgut basal lamina integrity was examined temporally at 3, 6, 18, 24, and 48 h following blood or protein feeding (A). Heat-treated midguts (70°C for 10 min in 1× phosphate-buffered saline [PBS]) were used as a positive (+) control sample. The CHP fluorescence signal was quantified with ImageJ for each sample, and used to determine the relative fluorescence at each time point following blood feeding (B) or protein feeding (C). CHP binding analysis was performed in three independent experiments under blood-fed conditions and in two independent experiments with protein feeding. For each time point, three or more midgut samples were examined by fluorescence microscopy with images analyzed using ImageJ. Relative fluorescence was calculated using the 0-h time point as the baseline measurement, then examined across multiple time points using a one-way analysis of variance (ANOVA) with a Holm-Sidak multiple-comparison test using GraphPad Prism 7 software. Asterisks denote significance (** * , P < 0.001; *** * , P < 0.0001).

    Article Snippet: To measure the potential disruption of collagen present on the midgut basal lamina, fluorescein-conjugated collagen hybridizing peptide (CHP) (Echelon Biosciences) that recognizes denatured collagen was diluted in 1× PBS (1:20, 5 μM).

    Techniques: Labeling, Positive Control, Fluorescence, Binding Assay, Microscopy, Software