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Qiagen flsirna
Effect of iron, iron chelation, FHsiRNA, and <t>FLsiRNA</t> on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were
Flsirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flsirna/product/Qiagen
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flsirna - by Bioz Stars, 2020-07
85/100 stars

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1) Product Images from "Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion"

Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

Journal: Investigative Ophthalmology & Visual Science

doi: 10.1167/iovs.09-3861

Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were
Figure Legend Snippet: Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were

Techniques Used: Transfection

Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled
Figure Legend Snippet: Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled

Techniques Used: Transfection, Immunolabeling

Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the
Figure Legend Snippet: Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the

Techniques Used: Transfection, Incubation, Labeling

Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine
Figure Legend Snippet: Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine

Techniques Used: Transfection, Incubation

The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression
Figure Legend Snippet: The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression

Techniques Used: Cell Culture, Transfection, Expressing, CTL Assay

Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free
Figure Legend Snippet: Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free

Techniques Used: Western Blot, Transfection, Incubation

Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel
Figure Legend Snippet: Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel

Techniques Used: Concentration Assay, Transfection

Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated
Figure Legend Snippet: Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated

Techniques Used: Transfection, Incubation, Lysis

Related Articles

Transfection:

Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
Article Snippet: .. Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Cell lysates from four 3.5-cm wells were combined for RNA extraction.

Cell Culture:

Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
Article Snippet: .. Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Cell lysates from four 3.5-cm wells were combined for RNA extraction.

CTL Assay:

Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
Article Snippet: .. Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Cell lysates from four 3.5-cm wells were combined for RNA extraction.

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    Qiagen flsirna
    Effect of iron, iron chelation, FHsiRNA, and <t>FLsiRNA</t> on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were
    Flsirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flsirna/product/Qiagen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flsirna - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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    Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection

    Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Immunolabeling

    Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Incubation, Labeling

    Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Incubation

    The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Cell Culture, Transfection, Expressing, CTL Assay

    Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Western Blot, Transfection, Incubation

    Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Concentration Assay, Transfection

    Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Incubation, Lysis