flsirna  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 78

    Structured Review

    Qiagen flsirna
    Effect of iron, iron chelation, FHsiRNA, and <t>FLsiRNA</t> on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were
    Flsirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flsirna/product/Qiagen
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flsirna - by Bioz Stars, 2019-12
    78/100 stars

    Images

    1) Product Images from "Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion"

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    Journal:

    doi: 10.1167/iovs.09-3861

    Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were
    Figure Legend Snippet: Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were

    Techniques Used: Transfection

    Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the
    Figure Legend Snippet: Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the

    Techniques Used: Transfection, Incubation, Labeling

    Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled
    Figure Legend Snippet: Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled

    Techniques Used: Transfection, Immunolabeling

    Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine
    Figure Legend Snippet: Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine

    Techniques Used: Transfection, Incubation

    The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression
    Figure Legend Snippet: The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression

    Techniques Used: Cell Culture, Transfection, Expressing, CTL Assay

    Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free
    Figure Legend Snippet: Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free

    Techniques Used: Western Blot, Transfection, Incubation

    Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel
    Figure Legend Snippet: Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel

    Techniques Used: Concentration Assay, Transfection

    Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated
    Figure Legend Snippet: Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated

    Techniques Used: Transfection, Incubation, Lysis

    Related Articles

    Transfection:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
    Article Snippet: De novo ferritin chain synthesis was monitored for each experiment to confirm knockdown. .. Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Cell lysates from four 3.5-cm wells were combined for RNA extraction.

    Cell Culture:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
    Article Snippet: De novo ferritin chain synthesis was monitored for each experiment to confirm knockdown. .. Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Cell lysates from four 3.5-cm wells were combined for RNA extraction.

    CTL Assay:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
    Article Snippet: De novo ferritin chain synthesis was monitored for each experiment to confirm knockdown. .. Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Cell lysates from four 3.5-cm wells were combined for RNA extraction.

    Real-time Polymerase Chain Reaction:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
    Article Snippet: Paragraph title: Reverse Transcription and Quantitative Real-Time PCR ... Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Concentration Assay:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Real-time PCR was performed with master mix (PowerSYBR Green; Applied Biosystems [ABI], Foster City, CA) in a thermocycler (iCycler iQ; Bio-Rad Laboratories, Hercules, CA).

    Spectrophotometry:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion
    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol. .. Cell lysates from four 3.5-cm wells were combined for RNA extraction.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 78
    Qiagen flsirna
    Effect of iron, iron chelation, FHsiRNA, and <t>FLsiRNA</t> on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were
    Flsirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flsirna/product/Qiagen
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flsirna - by Bioz Stars, 2019-12
    78/100 stars
      Buy from Supplier

    Image Search Results


    Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection

    Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Ferritin chain degradation after siRNA knockdown. Twenty-four hours after transfection with a control nontargeting siRNA, FHsiRNA, or FLsiRNA, LECs were incubated for 18 hours with 60 μCi translabel 35 S-methionine. After the labeling period, the

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Incubation, Labeling

    Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Transfection with FH- or FLsiRNA altered the levels of ferritin chains as determined by immunolocalization. The cells were transfected with control nontargeting siRNA ( A , D , G , J ), FHsiRNA ( B , E , H , K , M – O ), or FLsiRNA ( C , F , I , L ) and immunolabeled

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Immunolabeling

    Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of H- and L-chain siRNA on de novo ferritin subunit synthesis in LECs. ( A , B ) LECs were seeded in six-well plates, then transfected with nontargeting control or FH- or FLsiRNA. Two and 4 days later, the cells were incubated with 35 S-methionine

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Incubation

    The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: The effect of FH and FLsiRNA on FH and FL mRNA in cultured LECs. Total RNA was extracted from cultured LECs differentially transfected with siRNAs designed to suppress FH- or FLsiRNA expression or with nontargeting Ctl siRNA. Changes in the expression

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Cell Culture, Transfection, Expressing, CTL Assay

    Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of siRNA knockdown of ferritin H- and L-chain on transferrin receptor and iron uptake in LECs. ( A ) TfR Western blot. Twenty-four hours after transfection with a nontargeting control, FHsiRNA, or FLsiRNA, the cells were rinsed and incubated in serum-free

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Western Blot, Transfection, Incubation

    Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Concentration Assay, Transfection

    Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated

    Journal:

    Article Title: Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

    doi: 10.1167/iovs.09-3861

    Figure Lengend Snippet: Effect of H- and L-chain siRNA on iron incorporation into ferritin. ( A ) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with 59 Fe-transferrin. After cell lysis, proteins were precipitated

    Article Snippet: Total RNA was extracted from cultured LECs, 24 hours after transfection with CTL, FH, or FLsiRNA (RNeasy kit and Qiashredders; Qiagen, Valencia CA) according to the manufacturer's protocol.

    Techniques: Transfection, Incubation, Lysis