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flow cytometry instrument  (Partec)

 
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    Partec flow cytometry instrument
    Flow Cytometry Instrument, supplied by Partec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry/pm42270693-44-12-17?v=Partec
    Average 86 stars, based on 1 article reviews
    flow cytometry instrument - by Bioz Stars, 2026-07
    86/100 stars

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    Phenotypic and functional effects of a SLy1 knockout on splenic NK cells in dependence on p53. (A) Absolute NK cell count determined via flow <t>cytometry</t> in the spleen of mice of the specified genotypes. (B) Apoptotic (defined as Annexin V + ) NK cells. (C) Living (defined as 7-AAD - ) NK cells after four hours under culture conditions. (D) Expression of NKG2D, NK1.1 and NKp46 on NK cells. (E) In vitro cytotoxicity against LLC at a 25:1 effector:target ratio. Dead LLC cells were defined as 7-AAD + /Annexin V + . In all figures error bars represent standard error of the mean. ∗: p <0.05, ∗∗/##: p <0.01, ns, not significant; unpaired t-test. (F) Freshly isolated NK cells (1) attack and lyse LLC cells (2) in vitro (see arrow 3). Image was taken after four hours of incubation. Scale bar = 20 µm.
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    Image Search Results


    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining

    Phenotypic and functional effects of a SLy1 knockout on splenic NK cells in dependence on p53. (A) Absolute NK cell count determined via flow cytometry in the spleen of mice of the specified genotypes. (B) Apoptotic (defined as Annexin V + ) NK cells. (C) Living (defined as 7-AAD - ) NK cells after four hours under culture conditions. (D) Expression of NKG2D, NK1.1 and NKp46 on NK cells. (E) In vitro cytotoxicity against LLC at a 25:1 effector:target ratio. Dead LLC cells were defined as 7-AAD + /Annexin V + . In all figures error bars represent standard error of the mean. ∗: p <0.05, ∗∗/##: p <0.01, ns, not significant; unpaired t-test. (F) Freshly isolated NK cells (1) attack and lyse LLC cells (2) in vitro (see arrow 3). Image was taken after four hours of incubation. Scale bar = 20 µm.

    Journal: Frontiers in Immunology

    Article Title: SLy1-deficiency results in functional impaired, exhausted and senescent NK cells

    doi: 10.3389/fimmu.2026.1836862

    Figure Lengend Snippet: Phenotypic and functional effects of a SLy1 knockout on splenic NK cells in dependence on p53. (A) Absolute NK cell count determined via flow cytometry in the spleen of mice of the specified genotypes. (B) Apoptotic (defined as Annexin V + ) NK cells. (C) Living (defined as 7-AAD - ) NK cells after four hours under culture conditions. (D) Expression of NKG2D, NK1.1 and NKp46 on NK cells. (E) In vitro cytotoxicity against LLC at a 25:1 effector:target ratio. Dead LLC cells were defined as 7-AAD + /Annexin V + . In all figures error bars represent standard error of the mean. ∗: p <0.05, ∗∗/##: p <0.01, ns, not significant; unpaired t-test. (F) Freshly isolated NK cells (1) attack and lyse LLC cells (2) in vitro (see arrow 3). Image was taken after four hours of incubation. Scale bar = 20 µm.

    Article Snippet: 10 6 single cells were transferred into flow cytometry tubes (Sarstedt) and were washed once with cold PBS.

    Techniques: Functional Assay, Knock-Out, Cell Characterization, Flow Cytometry, Expressing, In Vitro, Isolation, Incubation