flow cytometry phycoerythrin pe conjugated mouse anti hiv core antibody  (Beckman Coulter)


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    Beckman Coulter flow cytometry phycoerythrin pe conjugated mouse anti hiv core antibody
    Proteasome inhibitors increase Casp8p41 levels and kill <t>HIV-infected</t> CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow <t>cytometry.</t> Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P
    Flow Cytometry Phycoerythrin Pe Conjugated Mouse Anti Hiv Core Antibody, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry phycoerythrin pe conjugated mouse anti hiv core antibody/product/Beckman Coulter
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    flow cytometry phycoerythrin pe conjugated mouse anti hiv core antibody - by Bioz Stars, 2020-09
    92/100 stars

    Related Products / Commonly Used Together

    allophycocyanin apc - conjugated rabbit anti-active caspase

    Images

    1) Product Images from "HIV Protease-Generated Casp8p41, When Bound and Inactivated by Bcl2, Is Degraded by the Proteasome"

    Article Title: HIV Protease-Generated Casp8p41, When Bound and Inactivated by Bcl2, Is Degraded by the Proteasome

    Journal: Journal of Virology

    doi: 10.1128/JVI.00037-18

    Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P
    Figure Legend Snippet: Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P

    Techniques Used: Infection, Flow Cytometry, Cytometry, Transfection, Plasmid Preparation, Expressing, Luciferase, Activity Assay, Permeability

    2) Product Images from "HIV Protease-Generated Casp8p41, When Bound and Inactivated by Bcl2, Is Degraded by the Proteasome"

    Article Title: HIV Protease-Generated Casp8p41, When Bound and Inactivated by Bcl2, Is Degraded by the Proteasome

    Journal: Journal of Virology

    doi: 10.1128/JVI.00037-18

    Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P
    Figure Legend Snippet: Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P

    Techniques Used: Infection, Flow Cytometry, Cytometry, Transfection, Plasmid Preparation, Expressing, Luciferase, Activity Assay, Permeability

    Related Articles

    other:

    Article Title:
    Article Snippet: The following antibodies were used for flow cytometry: phycoerythrin (PE)-conjugated mouse anti-HIV core antibody (Beckman Coulter) and allophycocyanin (APC)-conjugated rabbit anti-active caspase 3 antibody (BD Pharmingen; 1:100).

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    Beckman Coulter flow cytometry phycoerythrin pe conjugated mouse anti hiv core antibody
    Proteasome inhibitors increase Casp8p41 levels and kill <t>HIV-infected</t> CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow <t>cytometry.</t> Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P
    Flow Cytometry Phycoerythrin Pe Conjugated Mouse Anti Hiv Core Antibody, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry phycoerythrin pe conjugated mouse anti hiv core antibody/product/Beckman Coulter
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    flow cytometry phycoerythrin pe conjugated mouse anti hiv core antibody - by Bioz Stars, 2020-09
    92/100 stars
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    Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P

    Journal: Journal of Virology

    Article Title: HIV Protease-Generated Casp8p41, When Bound and Inactivated by Bcl2, Is Degraded by the Proteasome

    doi: 10.1128/JVI.00037-18

    Figure Lengend Snippet: Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P

    Article Snippet: The following antibodies were used for flow cytometry: phycoerythrin (PE)-conjugated mouse anti-HIV core antibody (Beckman Coulter) and allophycocyanin (APC)-conjugated rabbit anti-active caspase 3 antibody (BD Pharmingen; 1:100).

    Techniques: Infection, Flow Cytometry, Cytometry, Transfection, Plasmid Preparation, Expressing, Luciferase, Activity Assay, Permeability

    Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P

    Journal: Journal of Virology

    Article Title: HIV Protease-Generated Casp8p41, When Bound and Inactivated by Bcl2, Is Degraded by the Proteasome

    doi: 10.1128/JVI.00037-18

    Figure Lengend Snippet: Proteasome inhibitors increase Casp8p41 levels and kill HIV-infected CD4 T cell cultures more than uninfected cultures. (A) Uninfected primary CD4 + T cells were treated with bortezomib or ixazomib at increasing concentrations for 48 h, and cell death was assessed by activated caspase 3 detection by intracellular flow cytometry. Depicted are the means and SD of the results of two experiments. (B and C) Jurkat CD4 + T cells were transfected with empty vector or GFP-Casp8p41 and then treated with control (DMSO), bortezomib, or ixazomib, and the percentage of cells that were GFP positive was analyzed 6 h later. (C) Mean (plus SD) data from three independent replicates of the experiment shown in panel B compared by a Kruskal-Wallis test. (D) Primary CD4 T cells were infected with HIV IIIb , treated with control or bortezomib, and assessed for intracellular Casp8p41 expression by flow cytometry. (E) Primary CD4 T cells were infected with HIV-Luc; treated with DMSO, bortezomib, or ixazomib; and monitored for viability by ATP content over time. (F) Primary CD4 T cells infected with HIV-Luc were treated with DMSO, bortezomib, or ixazomib and monitored for luciferase activity over time. (G and H) Jurkat T cells were mock or GFP-HIV infected, treated with DMSO or ixazomib, and monitored for GFP (G) or cell death (by cell permeability dye) (H) over time. (I and J) Cell death selectively measured in GFP-expressing cells (I) and GFP-negative cells (J) in panels G and H was monitored over time. *, P

    Article Snippet: The following antibodies were used for flow cytometry: phycoerythrin (PE)-conjugated mouse anti-HIV core antibody (Beckman Coulter) and allophycocyanin (APC)-conjugated rabbit anti-active caspase 3 antibody (BD Pharmingen; 1:100).

    Techniques: Infection, Flow Cytometry, Cytometry, Transfection, Plasmid Preparation, Expressing, Luciferase, Activity Assay, Permeability