flow cytometry kit  (Millipore)


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  • 98
    Name:
    Flow Cytometry Kit
    Description:

    Catalog Number:
    apobrdu
    Price:
    None
    Applications:
    The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody.Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA. APOBRDU has been used in flow cytometry assay in human non-small cell lung cancer. APOBRDU has been used in cell cycle and apoptosis assay in HeLa cells, and in tunel assay human neuroblastoma cell lines.
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    Structured Review

    Millipore flow cytometry kit
    Flow Cytometry Kit

    https://www.bioz.com/result/flow cytometry kit/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flow cytometry kit - by Bioz Stars, 2020-05
    98/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Heme-Mediated Induction of CXCL10 and Depletion of CD34+ Progenitor Cells Is Toll-Like Receptor 4 Dependent
    Article Snippet: .. The Guava easyCyte flow cytometry system was used to quantify apoptosis with the TUNEL Kit for Flow Cytometry (Millipore, Billerica, MA). .. Cells were fixed and permeabilized using Guava TUNEL solution and apoptotic events were counted if they emitted a nucleated cell fluorescent signal, and exhibited the forward light scatter (FSC) intensity appropriate for a particle the size of a cell.

    Flow Cytometry:

    Article Title: IL-22 Confers EGFR-TKI Resistance in NSCLC via the AKT and ERK Signaling Pathways
    Article Snippet: .. Flow Cytometric Analysis PC9 and HCC827 cells were seeded on plates with six wells (2 × 104 cells per well), and treated with gefitinib (IC50), IL-22 neutralizing antibody (104 ng/ml), or IL-22 (50 ng/ml) for 48 h. The apoptotic rates of death were obtained using an apoptosis kit based on AnnexinV/PI (EMD Millipore, Billerica, MA, USA) according to the instructions of the manufacturer. .. Western Blot PC9 and HCC827 cells were lysed by RIPA protein extraction reagent, which was supplied with phenylmethanesulfonyl fluoride.

    Article Title: β-Sitosterol Protects against Myocardial Ischemia/Reperfusion Injury via Targeting PPARγ/NF-κB Signalling
    Article Snippet: .. Flow Cytometry for Cell Apoptosis Determination Cell apoptosis of H9c2 cells subjected to different treatments was evaluated using the Cell Apoptosis Detection kit (Sigma-Aldrich) by following the product manual. ..

    Article Title: Heme-Mediated Induction of CXCL10 and Depletion of CD34+ Progenitor Cells Is Toll-Like Receptor 4 Dependent
    Article Snippet: .. The Guava easyCyte flow cytometry system was used to quantify apoptosis with the TUNEL Kit for Flow Cytometry (Millipore, Billerica, MA). .. Cells were fixed and permeabilized using Guava TUNEL solution and apoptotic events were counted if they emitted a nucleated cell fluorescent signal, and exhibited the forward light scatter (FSC) intensity appropriate for a particle the size of a cell.

    Article Title: Silk fibroin peptide suppresses proliferation and induces apoptosis and cell cycle arrest in human lung cancer cells
    Article Snippet: .. The cell apoptosis assay was performed using the Apoptosis Analysis Kit (Sigma-Aldrich, St Louis, MO, USA), and apoptosis was analyzed using FACS flow cytometer. .. Western blotting was performed to determine the changes in protein expression in response to treatment with SFP.

    Cytometry:

    Article Title: Heme-Mediated Induction of CXCL10 and Depletion of CD34+ Progenitor Cells Is Toll-Like Receptor 4 Dependent
    Article Snippet: .. The Guava easyCyte flow cytometry system was used to quantify apoptosis with the TUNEL Kit for Flow Cytometry (Millipore, Billerica, MA). .. Cells were fixed and permeabilized using Guava TUNEL solution and apoptotic events were counted if they emitted a nucleated cell fluorescent signal, and exhibited the forward light scatter (FSC) intensity appropriate for a particle the size of a cell.

    Cell Culture:

    Article Title: Exosomes derived from human menstrual blood-derived stem cells alleviate fulminant hepatic failure
    Article Snippet: .. To analyze cell apoptosis, MenSC-Ex-treated AML12 cells that were cultured for 24 h with 44 μg/ml D-GalN and 100 ng/ml LPS were collected, centrifuged, and then stained with propidium iodide and Annexin V in the dark for 30 min at room temperature using a cell apoptosis Analysis Kit (Sigma-Aldrich). .. Cell apoptosis was then analyzed using flow cytometry.

    Apoptosis Assay:

    Article Title: Silk fibroin peptide suppresses proliferation and induces apoptosis and cell cycle arrest in human lung cancer cells
    Article Snippet: .. The cell apoptosis assay was performed using the Apoptosis Analysis Kit (Sigma-Aldrich, St Louis, MO, USA), and apoptosis was analyzed using FACS flow cytometer. .. Western blotting was performed to determine the changes in protein expression in response to treatment with SFP.

    Staining:

    Article Title: Exosomes derived from human menstrual blood-derived stem cells alleviate fulminant hepatic failure
    Article Snippet: .. To analyze cell apoptosis, MenSC-Ex-treated AML12 cells that were cultured for 24 h with 44 μg/ml D-GalN and 100 ng/ml LPS were collected, centrifuged, and then stained with propidium iodide and Annexin V in the dark for 30 min at room temperature using a cell apoptosis Analysis Kit (Sigma-Aldrich). .. Cell apoptosis was then analyzed using flow cytometry.

    Article Title: Immunoglobulin E induces colon cancer cell apoptosis via enhancing cyp27b1 expression
    Article Snippet: .. Cca cells were stained with an Annexin V reagent kit of apoptosis and propidium iodide (Sigma Aldrich) following the manufacturer’s instructions. .. The Annexin V+ , or Annexin V+ PI+ cells were regarded as apoptotic cells, which were assessed with a flow cytometer (FACSCanto II, BD Biosciences).

    FACS:

    Article Title: Silk fibroin peptide suppresses proliferation and induces apoptosis and cell cycle arrest in human lung cancer cells
    Article Snippet: .. The cell apoptosis assay was performed using the Apoptosis Analysis Kit (Sigma-Aldrich, St Louis, MO, USA), and apoptosis was analyzed using FACS flow cytometer. .. Western blotting was performed to determine the changes in protein expression in response to treatment with SFP.

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  • 93
    Millipore h2ax phosphorylation assay kit
    CRISPR-mediated restoration of FANCC . (A) The FANCC locus with mutation is indicated with a red asterisk. The mutation results in aberrant splicing ( red lines ) that cause exon 4 ( green box ) skipping. Normal splicing is indicated by the green dashed lines . Tan box represents exon 3, green box is exon 4, purple box is exon 5, and orange box is exon 8. (B) FANCC transcripts. The c.456+4A > T mutation-induced exon skipping results in deletion of exon 4. Gene correction results in restoration of exon 4 in the transcript. The green arrow indicates an allele-specific primer for the silent base changes that were introduced by donor-derived HDR. The blue primer is an exon 8-specific primer. (C) Allele-specific PCR of nickase- and nuclease-corrected cell clones. A representative gel of an allele-specific PCR showing normalized transcripts in the nuclease and nickase clones. The specificity of the primer set is evident because of absence of amplification in FA-C (FC) or wild-type (WT) cells. To ensure that cDNA was amplification grade, samples were subjected to PCR with GAPDH primers ( bottom ). Mw, molecular weight standards. (D) Sanger sequencing of gene-modified allele. At left is the start of exon 4, with blue arrows indicating the silent polymorphisms that were incorporated into the genome-targeting donor. At right is the junction ( shaded in blue ) of the restored exon 4 contiguous with exon 5. (E) FANCC activity. Graph is a representation of four experiments of the nuclease and two nickase clones utilizing flow cytometric analysis of phosphorylated <t>γ-H2AX</t> in FA cells that are untreated or treated with 2 m M hydroxyurea. Nuclease or nickase clones were assessed simultaneously and data are presented as the mean fluorescence intensity (MFI) of the phospho-γ-H2AX antibody signal. FC cells ( red bar ) were stained with an isotype control. Mean±SD are graphed and * is gene-corrected clones vs. controls with p
    H2ax Phosphorylation Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2ax phosphorylation assay kit/product/Millipore
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    h2ax phosphorylation assay kit - by Bioz Stars, 2020-05
    93/100 stars
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    91
    Millipore muse bcl 2 activation dual detection kit
    Effect of diallyl trisulfide on expression of <t>Bcl-2</t> in U87MG cells. The cells were treated with 100 µM DATS for 24 and 48 h. Bcl-2 expression was analyzed using a Muse™ Bcl-2 Activation Dual Detection Kit. The samples were analyzed by flow cytometry. a One set of representative results is shown. b Each point represents the mean ± SD of three independent experiments
    Muse Bcl 2 Activation Dual Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/muse bcl 2 activation dual detection kit/product/Millipore
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    muse bcl 2 activation dual detection kit - by Bioz Stars, 2020-05
    91/100 stars
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    93
    Millipore flowcellect egfr mapk kit
    Inhibition of the <t>EGFR/MAPK</t> pathway after compound 1 treatment in MDA-MB-231 and MCF-7 cells. MDA-MB-231 and MCF-7 cells were incubated with compound 1 at IC 0 and IC 50 for 4 hours, and human recombinant EGF (hrEGF) was used as a positive control. Bar graphs of fluorescence intensity of EGFR and MAPK in MDA-MB-231 cells (a) and MCF-7 cells (b) were compared with the control (as folds) shown by using Guava® Flow Cytometry easyCyte ™ Systems. The statistical significance values compared to the control (without treatment) are marked with asterisks, ∗∗ p
    Flowcellect Egfr Mapk Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flowcellect egfr mapk kit/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flowcellect egfr mapk kit - by Bioz Stars, 2020-05
    93/100 stars
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    93
    Millipore muse annexin v dead cell kit
    FCP induced apoptosis of A549 cells. (A) A549 cells were treated with FCP at the indicated concentrations for 24 h, and apoptosis was assessed by Annexin V-propidium iodide double staining using Mini Flow Cytometry Muse™ Cell Analyzer. (B) Quantitative data indicate that total apoptosis significantly increased in 182 and 364 µg/ml FCP-treated cells Data were expressed as the mean ± standard deviation of three independent experiments (*P
    Muse Annexin V Dead Cell Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/muse annexin v dead cell kit/product/Millipore
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    muse annexin v dead cell kit - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    CRISPR-mediated restoration of FANCC . (A) The FANCC locus with mutation is indicated with a red asterisk. The mutation results in aberrant splicing ( red lines ) that cause exon 4 ( green box ) skipping. Normal splicing is indicated by the green dashed lines . Tan box represents exon 3, green box is exon 4, purple box is exon 5, and orange box is exon 8. (B) FANCC transcripts. The c.456+4A > T mutation-induced exon skipping results in deletion of exon 4. Gene correction results in restoration of exon 4 in the transcript. The green arrow indicates an allele-specific primer for the silent base changes that were introduced by donor-derived HDR. The blue primer is an exon 8-specific primer. (C) Allele-specific PCR of nickase- and nuclease-corrected cell clones. A representative gel of an allele-specific PCR showing normalized transcripts in the nuclease and nickase clones. The specificity of the primer set is evident because of absence of amplification in FA-C (FC) or wild-type (WT) cells. To ensure that cDNA was amplification grade, samples were subjected to PCR with GAPDH primers ( bottom ). Mw, molecular weight standards. (D) Sanger sequencing of gene-modified allele. At left is the start of exon 4, with blue arrows indicating the silent polymorphisms that were incorporated into the genome-targeting donor. At right is the junction ( shaded in blue ) of the restored exon 4 contiguous with exon 5. (E) FANCC activity. Graph is a representation of four experiments of the nuclease and two nickase clones utilizing flow cytometric analysis of phosphorylated γ-H2AX in FA cells that are untreated or treated with 2 m M hydroxyurea. Nuclease or nickase clones were assessed simultaneously and data are presented as the mean fluorescence intensity (MFI) of the phospho-γ-H2AX antibody signal. FC cells ( red bar ) were stained with an isotype control. Mean±SD are graphed and * is gene-corrected clones vs. controls with p

    Journal: Human Gene Therapy

    Article Title: Fanconi Anemia Gene Editing by the CRISPR/Cas9 System

    doi: 10.1089/hum.2014.111

    Figure Lengend Snippet: CRISPR-mediated restoration of FANCC . (A) The FANCC locus with mutation is indicated with a red asterisk. The mutation results in aberrant splicing ( red lines ) that cause exon 4 ( green box ) skipping. Normal splicing is indicated by the green dashed lines . Tan box represents exon 3, green box is exon 4, purple box is exon 5, and orange box is exon 8. (B) FANCC transcripts. The c.456+4A > T mutation-induced exon skipping results in deletion of exon 4. Gene correction results in restoration of exon 4 in the transcript. The green arrow indicates an allele-specific primer for the silent base changes that were introduced by donor-derived HDR. The blue primer is an exon 8-specific primer. (C) Allele-specific PCR of nickase- and nuclease-corrected cell clones. A representative gel of an allele-specific PCR showing normalized transcripts in the nuclease and nickase clones. The specificity of the primer set is evident because of absence of amplification in FA-C (FC) or wild-type (WT) cells. To ensure that cDNA was amplification grade, samples were subjected to PCR with GAPDH primers ( bottom ). Mw, molecular weight standards. (D) Sanger sequencing of gene-modified allele. At left is the start of exon 4, with blue arrows indicating the silent polymorphisms that were incorporated into the genome-targeting donor. At right is the junction ( shaded in blue ) of the restored exon 4 contiguous with exon 5. (E) FANCC activity. Graph is a representation of four experiments of the nuclease and two nickase clones utilizing flow cytometric analysis of phosphorylated γ-H2AX in FA cells that are untreated or treated with 2 m M hydroxyurea. Nuclease or nickase clones were assessed simultaneously and data are presented as the mean fluorescence intensity (MFI) of the phospho-γ-H2AX antibody signal. FC cells ( red bar ) were stained with an isotype control. Mean±SD are graphed and * is gene-corrected clones vs. controls with p

    Article Snippet: H2AX staining was performed on cells seeded at a concentration of 120,000 total cells in a T25 flask in the presence of 2 m M hydroxyurea (Sigma) for 48 hr using the H2AX phosphorylation assay kit according to the manufacturer's instructions (EMD Millipore, Billerica, MA).

    Techniques: CRISPR, Mutagenesis, Derivative Assay, Polymerase Chain Reaction, Clone Assay, Amplification, Molecular Weight, Sequencing, Modification, Activity Assay, Flow Cytometry, Fluorescence, Staining

    Effect of diallyl trisulfide on expression of Bcl-2 in U87MG cells. The cells were treated with 100 µM DATS for 24 and 48 h. Bcl-2 expression was analyzed using a Muse™ Bcl-2 Activation Dual Detection Kit. The samples were analyzed by flow cytometry. a One set of representative results is shown. b Each point represents the mean ± SD of three independent experiments

    Journal: Amino Acids

    Article Title: A possible mechanism of inhibition of U87MG and SH-SY5Y cancer cell proliferation by diallyl trisulfide and other aspects of its activity

    doi: 10.1007/s00726-017-2484-4

    Figure Lengend Snippet: Effect of diallyl trisulfide on expression of Bcl-2 in U87MG cells. The cells were treated with 100 µM DATS for 24 and 48 h. Bcl-2 expression was analyzed using a Muse™ Bcl-2 Activation Dual Detection Kit. The samples were analyzed by flow cytometry. a One set of representative results is shown. b Each point represents the mean ± SD of three independent experiments

    Article Snippet: Effect of diallyl trisulfide on the Bcl-2 expression in human glioblastoma (U87MG) and neuroblastoma (SH-SY5Y) cell lines A Muse™ Bcl-2 Activation Dual Detection Kit was used to measure the percentage of Bcl-2 protein activation in SH-SY5Y and U87MG cells.

    Techniques: Expressing, Activation Assay, Flow Cytometry, Cytometry

    Effect of diallyl trisulfide on expression of Bcl-2 in SH-SY5Y cells. The cells were treated with 100 µM DATS for 24 and 48 h. Bcl-2 expression was analyzed using a Muse™ Bcl-2 Activation Dual Detection Kit. The samples were analyzed by flow cytometry. a One set of representative results is shown. b Each point represents the mean ± SD of three independent experiments

    Journal: Amino Acids

    Article Title: A possible mechanism of inhibition of U87MG and SH-SY5Y cancer cell proliferation by diallyl trisulfide and other aspects of its activity

    doi: 10.1007/s00726-017-2484-4

    Figure Lengend Snippet: Effect of diallyl trisulfide on expression of Bcl-2 in SH-SY5Y cells. The cells were treated with 100 µM DATS for 24 and 48 h. Bcl-2 expression was analyzed using a Muse™ Bcl-2 Activation Dual Detection Kit. The samples were analyzed by flow cytometry. a One set of representative results is shown. b Each point represents the mean ± SD of three independent experiments

    Article Snippet: Effect of diallyl trisulfide on the Bcl-2 expression in human glioblastoma (U87MG) and neuroblastoma (SH-SY5Y) cell lines A Muse™ Bcl-2 Activation Dual Detection Kit was used to measure the percentage of Bcl-2 protein activation in SH-SY5Y and U87MG cells.

    Techniques: Expressing, Activation Assay, Flow Cytometry, Cytometry

    Inhibition of the EGFR/MAPK pathway after compound 1 treatment in MDA-MB-231 and MCF-7 cells. MDA-MB-231 and MCF-7 cells were incubated with compound 1 at IC 0 and IC 50 for 4 hours, and human recombinant EGF (hrEGF) was used as a positive control. Bar graphs of fluorescence intensity of EGFR and MAPK in MDA-MB-231 cells (a) and MCF-7 cells (b) were compared with the control (as folds) shown by using Guava® Flow Cytometry easyCyte ™ Systems. The statistical significance values compared to the control (without treatment) are marked with asterisks, ∗∗ p

    Journal: BioMed Research International

    Article Title: Dihydrochalcone Derivative Induces Breast Cancer Cell Apoptosis via Intrinsic, Extrinsic, and ER Stress Pathways but Abolishes EGFR/MAPK Pathway

    doi: 10.1155/2019/7298539

    Figure Lengend Snippet: Inhibition of the EGFR/MAPK pathway after compound 1 treatment in MDA-MB-231 and MCF-7 cells. MDA-MB-231 and MCF-7 cells were incubated with compound 1 at IC 0 and IC 50 for 4 hours, and human recombinant EGF (hrEGF) was used as a positive control. Bar graphs of fluorescence intensity of EGFR and MAPK in MDA-MB-231 cells (a) and MCF-7 cells (b) were compared with the control (as folds) shown by using Guava® Flow Cytometry easyCyte ™ Systems. The statistical significance values compared to the control (without treatment) are marked with asterisks, ∗∗ p

    Article Snippet: Determination of EGFR/MAPK Pathway by FlowCellect™ EGFR/MAPK Activation Detection Kit After the cells were treated with compound 1 for 4 hours, the cells were collected and then a Millipore FlowCellect™ EGFR/MAPK kit was used as has been described in the protocol.

    Techniques: Inhibition, Multiple Displacement Amplification, Incubation, Recombinant, Positive Control, Fluorescence, Flow Cytometry, Cytometry

    FCP induced apoptosis of A549 cells. (A) A549 cells were treated with FCP at the indicated concentrations for 24 h, and apoptosis was assessed by Annexin V-propidium iodide double staining using Mini Flow Cytometry Muse™ Cell Analyzer. (B) Quantitative data indicate that total apoptosis significantly increased in 182 and 364 µg/ml FCP-treated cells Data were expressed as the mean ± standard deviation of three independent experiments (*P

    Journal: Oncology Letters

    Article Title: Flavonoids isolated from Citrus platymamma induced G2/M cell cycle arrest and apoptosis in A549 human lung cancer cells

    doi: 10.3892/ol.2016.4793

    Figure Lengend Snippet: FCP induced apoptosis of A549 cells. (A) A549 cells were treated with FCP at the indicated concentrations for 24 h, and apoptosis was assessed by Annexin V-propidium iodide double staining using Mini Flow Cytometry Muse™ Cell Analyzer. (B) Quantitative data indicate that total apoptosis significantly increased in 182 and 364 µg/ml FCP-treated cells Data were expressed as the mean ± standard deviation of three independent experiments (*P

    Article Snippet: Muse™ Annexin V & Dead Cell kit was purchased from EMD Millipore.

    Techniques: Double Staining, Flow Cytometry, Cytometry, Standard Deviation