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Miltenyi Biotec flow cytometry
Flow Cytometry, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1102 article reviews
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flow cytometry - by Bioz Stars, 2020-05
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Irradiation:

Article Title: Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice
Article Snippet: .. The CFSE-labeled lymphocytes from the transplant recipients were incubated for 5 days (120 h) with the unlabeled irradiated naïve splenocytes/lymphocytes The cells were washed and prepared for flow cytometry analysis by incubation with the antibodies CD3, CD4, and CD8 (Miltenyi Biotec). .. The viability dye 7-Amino-Actinomycin D (7AAD) (Sigma-Aldrich) was used to exclude dead cells from the analysis.

Flow Cytometry:

Article Title: C-terminal truncated hepatitis B virus X protein promotes hepatocellular carcinogenesis through induction of cancer and stem cell-like properties
Article Snippet: .. Flow cytometry and cell sorting Flow cytometry analysis or flow cytometry cell sorting was conducted using PE-conjugated mouse anti-human CD133 (Miltenyi Biotec) or its respective isotype control. .. ALDEFLUOR reagent (Stem Cell Technologies) was used for the immunofluorescent detection of intracellular ALDH enzyme activity.

Article Title: Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice
Article Snippet: .. The CFSE-labeled lymphocytes from the transplant recipients were incubated for 5 days (120 h) with the unlabeled irradiated naïve splenocytes/lymphocytes The cells were washed and prepared for flow cytometry analysis by incubation with the antibodies CD3, CD4, and CD8 (Miltenyi Biotec). .. The viability dye 7-Amino-Actinomycin D (7AAD) (Sigma-Aldrich) was used to exclude dead cells from the analysis.

Article Title: Tumor cell‐derived angiopoietin‐like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells, et al. Tumor cell‐derived angiopoietin‐like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells
Article Snippet: .. 2.7 Flow cytometry Cells were suspended in MACS buffer (Miltenyi Biotec) and stained with the following Abs: anti‐GLUT3 (ab15311; Abcam), anti‐integrin α5β1 (MAB1969; Millipore), anti‐integrin αvβ3 (MAB1976Z; Millipore), anti‐integrin αvβ5 (MAB1961; Millipore), and anti‐integrin α9β1 (Sc‐59969; Santa Cruz Biotechnology). ..

Article Title: Suppression of miR-204 enables oral squamous cell carcinomas to promote cancer stemness, EMT traits, and lymph node metastasis
Article Snippet: .. CD44 staining by flow cytometry analysis Cells were stained with anti-CD44 antibody conjugated to phycoerythrin (Miltenyi Biotech., Auburn, CA, USA), with labeling according to the manufacturer's instructions. .. Red ( > 650 nm) fluorescence emission from 10,000 cells illuminated with blue (488 nm) excitation light was measured with a FACSCalibur (Becton Dickinson) using CellQuest software.

Article Title: PDGFR? and CD51 mark human Nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion
Article Snippet: .. For flow cytometry sorting, cells were enriched by immunomagnetic depletion using anti-CD45 magnetic beads (Miltenyi Biotec) according to the manufacturer’s recommendations. .. Cells were sorted on a FACSAria (BD) to > 95% purity.

Article Title: Nucleotide ecto-enzyme metabolic pattern and spatial distribution in calcific aortic valve disease; its relation to pathological changes and clinical presentation
Article Snippet: .. Flow cytometry analysis Cells were resuspended in MACS buffer, preincubated with FcR Blocking Reagent (Miltenyi Biotech) and stained with specific antibodies that origin was described in the Supplementary material online. ..

Article Title: Aptamer-PEG-modified Fe3O4@Mn as a novel T1- and T2- dual-model MRI contrast agent targeting hypoxia-induced cancer stem cells
Article Snippet: .. Flow cytometry analysis Cells (5 × 106 ) cultured under hypoxic and normoxic conditions were harvested, disaggregated into single-cell suspensions, and stained with 1.25 μg/ml mouse anti-human phycoerythrin (PE)-labelled CD133 (clone AC133, Miltenyi Biotec. .. After incubation, the samples were washed with PBS and analysed by FACS Aria II (Becton Dickinson, USA).

Magnetic Beads:

Article Title: PDGFR? and CD51 mark human Nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion
Article Snippet: .. For flow cytometry sorting, cells were enriched by immunomagnetic depletion using anti-CD45 magnetic beads (Miltenyi Biotec) according to the manufacturer’s recommendations. .. Cells were sorted on a FACSAria (BD) to > 95% purity.

Cytometry:

Article Title: C-terminal truncated hepatitis B virus X protein promotes hepatocellular carcinogenesis through induction of cancer and stem cell-like properties
Article Snippet: .. Flow cytometry and cell sorting Flow cytometry analysis or flow cytometry cell sorting was conducted using PE-conjugated mouse anti-human CD133 (Miltenyi Biotec) or its respective isotype control. .. ALDEFLUOR reagent (Stem Cell Technologies) was used for the immunofluorescent detection of intracellular ALDH enzyme activity.

Article Title: Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice
Article Snippet: .. The CFSE-labeled lymphocytes from the transplant recipients were incubated for 5 days (120 h) with the unlabeled irradiated naïve splenocytes/lymphocytes The cells were washed and prepared for flow cytometry analysis by incubation with the antibodies CD3, CD4, and CD8 (Miltenyi Biotec). .. The viability dye 7-Amino-Actinomycin D (7AAD) (Sigma-Aldrich) was used to exclude dead cells from the analysis.

Article Title: Suppression of miR-204 enables oral squamous cell carcinomas to promote cancer stemness, EMT traits, and lymph node metastasis
Article Snippet: .. CD44 staining by flow cytometry analysis Cells were stained with anti-CD44 antibody conjugated to phycoerythrin (Miltenyi Biotech., Auburn, CA, USA), with labeling according to the manufacturer's instructions. .. Red ( > 650 nm) fluorescence emission from 10,000 cells illuminated with blue (488 nm) excitation light was measured with a FACSCalibur (Becton Dickinson) using CellQuest software.

Article Title: PDGFR? and CD51 mark human Nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion
Article Snippet: .. For flow cytometry sorting, cells were enriched by immunomagnetic depletion using anti-CD45 magnetic beads (Miltenyi Biotec) according to the manufacturer’s recommendations. .. Cells were sorted on a FACSAria (BD) to > 95% purity.

Article Title: Aptamer-PEG-modified Fe3O4@Mn as a novel T1- and T2- dual-model MRI contrast agent targeting hypoxia-induced cancer stem cells
Article Snippet: .. Flow cytometry analysis Cells (5 × 106 ) cultured under hypoxic and normoxic conditions were harvested, disaggregated into single-cell suspensions, and stained with 1.25 μg/ml mouse anti-human phycoerythrin (PE)-labelled CD133 (clone AC133, Miltenyi Biotec. .. After incubation, the samples were washed with PBS and analysed by FACS Aria II (Becton Dickinson, USA).

Labeling:

Article Title: Suppression of miR-204 enables oral squamous cell carcinomas to promote cancer stemness, EMT traits, and lymph node metastasis
Article Snippet: .. CD44 staining by flow cytometry analysis Cells were stained with anti-CD44 antibody conjugated to phycoerythrin (Miltenyi Biotech., Auburn, CA, USA), with labeling according to the manufacturer's instructions. .. Red ( > 650 nm) fluorescence emission from 10,000 cells illuminated with blue (488 nm) excitation light was measured with a FACSCalibur (Becton Dickinson) using CellQuest software.

Incubation:

Article Title: Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice
Article Snippet: .. The CFSE-labeled lymphocytes from the transplant recipients were incubated for 5 days (120 h) with the unlabeled irradiated naïve splenocytes/lymphocytes The cells were washed and prepared for flow cytometry analysis by incubation with the antibodies CD3, CD4, and CD8 (Miltenyi Biotec). .. The viability dye 7-Amino-Actinomycin D (7AAD) (Sigma-Aldrich) was used to exclude dead cells from the analysis.

Magnetic Cell Separation:

Article Title: Tumor cell‐derived angiopoietin‐like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells, et al. Tumor cell‐derived angiopoietin‐like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells
Article Snippet: .. 2.7 Flow cytometry Cells were suspended in MACS buffer (Miltenyi Biotec) and stained with the following Abs: anti‐GLUT3 (ab15311; Abcam), anti‐integrin α5β1 (MAB1969; Millipore), anti‐integrin αvβ3 (MAB1976Z; Millipore), anti‐integrin αvβ5 (MAB1961; Millipore), and anti‐integrin α9β1 (Sc‐59969; Santa Cruz Biotechnology). ..

Article Title: Nucleotide ecto-enzyme metabolic pattern and spatial distribution in calcific aortic valve disease; its relation to pathological changes and clinical presentation
Article Snippet: .. Flow cytometry analysis Cells were resuspended in MACS buffer, preincubated with FcR Blocking Reagent (Miltenyi Biotech) and stained with specific antibodies that origin was described in the Supplementary material online. ..

Cell Culture:

Article Title: Aptamer-PEG-modified Fe3O4@Mn as a novel T1- and T2- dual-model MRI contrast agent targeting hypoxia-induced cancer stem cells
Article Snippet: .. Flow cytometry analysis Cells (5 × 106 ) cultured under hypoxic and normoxic conditions were harvested, disaggregated into single-cell suspensions, and stained with 1.25 μg/ml mouse anti-human phycoerythrin (PE)-labelled CD133 (clone AC133, Miltenyi Biotec. .. After incubation, the samples were washed with PBS and analysed by FACS Aria II (Becton Dickinson, USA).

FACS:

Article Title: C-terminal truncated hepatitis B virus X protein promotes hepatocellular carcinogenesis through induction of cancer and stem cell-like properties
Article Snippet: .. Flow cytometry and cell sorting Flow cytometry analysis or flow cytometry cell sorting was conducted using PE-conjugated mouse anti-human CD133 (Miltenyi Biotec) or its respective isotype control. .. ALDEFLUOR reagent (Stem Cell Technologies) was used for the immunofluorescent detection of intracellular ALDH enzyme activity.

Staining:

Article Title: Tumor cell‐derived angiopoietin‐like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells, et al. Tumor cell‐derived angiopoietin‐like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells
Article Snippet: .. 2.7 Flow cytometry Cells were suspended in MACS buffer (Miltenyi Biotec) and stained with the following Abs: anti‐GLUT3 (ab15311; Abcam), anti‐integrin α5β1 (MAB1969; Millipore), anti‐integrin αvβ3 (MAB1976Z; Millipore), anti‐integrin αvβ5 (MAB1961; Millipore), and anti‐integrin α9β1 (Sc‐59969; Santa Cruz Biotechnology). ..

Article Title: Suppression of miR-204 enables oral squamous cell carcinomas to promote cancer stemness, EMT traits, and lymph node metastasis
Article Snippet: .. CD44 staining by flow cytometry analysis Cells were stained with anti-CD44 antibody conjugated to phycoerythrin (Miltenyi Biotech., Auburn, CA, USA), with labeling according to the manufacturer's instructions. .. Red ( > 650 nm) fluorescence emission from 10,000 cells illuminated with blue (488 nm) excitation light was measured with a FACSCalibur (Becton Dickinson) using CellQuest software.

Article Title: Nucleotide ecto-enzyme metabolic pattern and spatial distribution in calcific aortic valve disease; its relation to pathological changes and clinical presentation
Article Snippet: .. Flow cytometry analysis Cells were resuspended in MACS buffer, preincubated with FcR Blocking Reagent (Miltenyi Biotech) and stained with specific antibodies that origin was described in the Supplementary material online. ..

Article Title: Aptamer-PEG-modified Fe3O4@Mn as a novel T1- and T2- dual-model MRI contrast agent targeting hypoxia-induced cancer stem cells
Article Snippet: .. Flow cytometry analysis Cells (5 × 106 ) cultured under hypoxic and normoxic conditions were harvested, disaggregated into single-cell suspensions, and stained with 1.25 μg/ml mouse anti-human phycoerythrin (PE)-labelled CD133 (clone AC133, Miltenyi Biotec. .. After incubation, the samples were washed with PBS and analysed by FACS Aria II (Becton Dickinson, USA).

Blocking Assay:

Article Title: Nucleotide ecto-enzyme metabolic pattern and spatial distribution in calcific aortic valve disease; its relation to pathological changes and clinical presentation
Article Snippet: .. Flow cytometry analysis Cells were resuspended in MACS buffer, preincubated with FcR Blocking Reagent (Miltenyi Biotech) and stained with specific antibodies that origin was described in the Supplementary material online. ..

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  • 91
    Miltenyi Biotec flow cytometry cell viability
    The effect of prolonged encapsulation on Tie2-iBMMs. a-d Alginate capsules seeded with Tie2-iBMMs at days: a 1, b 7, c 14 and d 21 postencapsulation. e Tie2-iBMM retention within the capsules was quantified up to day 21 following encapsulation ( n = 5/group, P = n/s by one-way ANOVA). f Flow cytometric analysis of cell viability using annexin V/PI staining (Q1 = cell debris; Q2 = dead cells; Q3 = apoptosing cells; Q4 = live cells). g Quantification of annexin V/PI staining of encapsulated Tie2-iBMMs at days 1, 2, 3 and 7 post-encapsulation ( n = 4/group, P = n/s by two-way ANOVA and Bonferroni post-test). h Measurement of cell phenotype by flow <t>cytometry</t> in nTie2-iBMMs (green) and eTie2-iBMMs (grey) up to 21 days in vitro ( n = 5/group, P = n/s P
    Flow Cytometry Cell Viability, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry cell viability/product/Miltenyi Biotec
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    flow cytometry cell viability - by Bioz Stars, 2020-05
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    96
    Miltenyi Biotec flow cytometric analysis
    Flow <t>cytometric</t> viability analysis of B103 cells following transfection with ExoY and treatment with EF or CyaA. A, B103 cells were transfected with pcDNA3.1-ExoY or pcDNA 3.1-ExoY-K81M and incubated for 72 h and cell viability was analyzed by annexin V/PI staining. B, B103 cells were treated with 10 µg/ml CyaA holotoxin, 10 µg/ml CyaA mutant or urea carrier for 72 h and cell viability was analyzed by annexin V/PI staining. C, B103 cells were treated with 1 µg/ml EF, 2.3 µg/ml PA or a combination of 1 µg/ml EF +2.3 µg/ml PA for 72 h and cell viability was analyzed by annexin V/PI staining. N.s., not significant; *, p
    Flow Cytometric Analysis, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric analysis/product/Miltenyi Biotec
    Average 96 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    flow cytometric analysis - by Bioz Stars, 2020-05
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    92
    Miltenyi Biotec flow cytometry data
    Analysis of the expression of Toll-like receptor 2 (TLR2) by flow <t>cytometry</t> in natural killer (NK) cell CD56 bright and CD56 dim subsets . ( A ) Representative flow cytometry histogram of surface expression of TLR2 on CD56 bright and CD56 dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-TLR2. ( B ) Surface TLR2 expression shown as median and interquartile range in all three studied groups. ( C ) Representative flow cytometry histogram of intracellular expression of TLR2 for the same samples represented in ( A) . Black line: isotype control; colored line: anti-TLR2. ( D ) Intracellular TLR2 expression shown as median and interquartile range in all three studied groups. ** P
    Flow Cytometry Data, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry data/product/Miltenyi Biotec
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    flow cytometry data - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    The effect of prolonged encapsulation on Tie2-iBMMs. a-d Alginate capsules seeded with Tie2-iBMMs at days: a 1, b 7, c 14 and d 21 postencapsulation. e Tie2-iBMM retention within the capsules was quantified up to day 21 following encapsulation ( n = 5/group, P = n/s by one-way ANOVA). f Flow cytometric analysis of cell viability using annexin V/PI staining (Q1 = cell debris; Q2 = dead cells; Q3 = apoptosing cells; Q4 = live cells). g Quantification of annexin V/PI staining of encapsulated Tie2-iBMMs at days 1, 2, 3 and 7 post-encapsulation ( n = 4/group, P = n/s by two-way ANOVA and Bonferroni post-test). h Measurement of cell phenotype by flow cytometry in nTie2-iBMMs (green) and eTie2-iBMMs (grey) up to 21 days in vitro ( n = 5/group, P = n/s P

    Journal: NPJ Regenerative Medicine

    Article Title: Encapsulation of macrophages enhances their retention and angiogenic potential

    doi: 10.1038/s41536-019-0068-5

    Figure Lengend Snippet: The effect of prolonged encapsulation on Tie2-iBMMs. a-d Alginate capsules seeded with Tie2-iBMMs at days: a 1, b 7, c 14 and d 21 postencapsulation. e Tie2-iBMM retention within the capsules was quantified up to day 21 following encapsulation ( n = 5/group, P = n/s by one-way ANOVA). f Flow cytometric analysis of cell viability using annexin V/PI staining (Q1 = cell debris; Q2 = dead cells; Q3 = apoptosing cells; Q4 = live cells). g Quantification of annexin V/PI staining of encapsulated Tie2-iBMMs at days 1, 2, 3 and 7 post-encapsulation ( n = 4/group, P = n/s by two-way ANOVA and Bonferroni post-test). h Measurement of cell phenotype by flow cytometry in nTie2-iBMMs (green) and eTie2-iBMMs (grey) up to 21 days in vitro ( n = 5/group, P = n/s P

    Article Snippet: Flow cytometry Cell viability and phenotype were assessed using either a MACSQuant (Miltenyi Biotec, UK) or AttuneNxT (Thermo Scientific, UK) flow cytometer.

    Techniques: Flow Cytometry, Staining, Cytometry, In Vitro

    Flow cytometric viability analysis of B103 cells following transfection with ExoY and treatment with EF or CyaA. A, B103 cells were transfected with pcDNA3.1-ExoY or pcDNA 3.1-ExoY-K81M and incubated for 72 h and cell viability was analyzed by annexin V/PI staining. B, B103 cells were treated with 10 µg/ml CyaA holotoxin, 10 µg/ml CyaA mutant or urea carrier for 72 h and cell viability was analyzed by annexin V/PI staining. C, B103 cells were treated with 1 µg/ml EF, 2.3 µg/ml PA or a combination of 1 µg/ml EF +2.3 µg/ml PA for 72 h and cell viability was analyzed by annexin V/PI staining. N.s., not significant; *, p

    Journal: Biochemical and biophysical research communications

    Article Title: ExoY from Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for cGMP and cUMP formation

    doi: 10.1016/j.bbrc.2014.06.088

    Figure Lengend Snippet: Flow cytometric viability analysis of B103 cells following transfection with ExoY and treatment with EF or CyaA. A, B103 cells were transfected with pcDNA3.1-ExoY or pcDNA 3.1-ExoY-K81M and incubated for 72 h and cell viability was analyzed by annexin V/PI staining. B, B103 cells were treated with 10 µg/ml CyaA holotoxin, 10 µg/ml CyaA mutant or urea carrier for 72 h and cell viability was analyzed by annexin V/PI staining. C, B103 cells were treated with 1 µg/ml EF, 2.3 µg/ml PA or a combination of 1 µg/ml EF +2.3 µg/ml PA for 72 h and cell viability was analyzed by annexin V/PI staining. N.s., not significant; *, p

    Article Snippet: The flow-cytometric analysis was performed using a MACS Quant Analyzer with MAQS Quant running buffer (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Flow Cytometry, Transfection, Incubation, Staining, Mutagenesis

    RST1 isolates induce expression of multiple interferons. Human PBMCs (4×10 6 ) were co-incubated for 20 hrs with RST1 (black bars) or RST3 (white bars) B. burgdorferi clinical isolates (4×10 7 ) (MOI = 10∶1). Concentrations of human IFN-α ( A ), IFN-λ1/IL29 ( B ), or IFN-γ ( C ) proteins in cell-free culture supernatants were quantitated by ELISA ( A , B ) or cytometric bead array ( C ). Columns represent the mean ± SD of values from three blood donors assessed in independent experiments, with the exception of ( B ) which represents results from a single donor. Statistical analysis was performed using a non-parametric, two-tailed Mann-Whitney U-test.

    Journal: PLoS ONE

    Article Title: Induction of Type I and Type III Interferons by Borrelia burgdorferi Correlates with Pathogenesis and Requires Linear Plasmid 36

    doi: 10.1371/journal.pone.0100174

    Figure Lengend Snippet: RST1 isolates induce expression of multiple interferons. Human PBMCs (4×10 6 ) were co-incubated for 20 hrs with RST1 (black bars) or RST3 (white bars) B. burgdorferi clinical isolates (4×10 7 ) (MOI = 10∶1). Concentrations of human IFN-α ( A ), IFN-λ1/IL29 ( B ), or IFN-γ ( C ) proteins in cell-free culture supernatants were quantitated by ELISA ( A , B ) or cytometric bead array ( C ). Columns represent the mean ± SD of values from three blood donors assessed in independent experiments, with the exception of ( B ) which represents results from a single donor. Statistical analysis was performed using a non-parametric, two-tailed Mann-Whitney U-test.

    Article Snippet: Total numbers of GFP+ PBMCs were determined by flow cytometric analysis of 100,000 to 300,000 events using a MACSQuant Analyzer and MACSQuantify software (Miltenyi Biotec).

    Techniques: Expressing, Incubation, Enzyme-linked Immunosorbent Assay, Two Tailed Test, MANN-WHITNEY

    The presence of lp36 is required for IFN-α and IFN-λ induction. Human PBMCs (4×10 6 ) were co-incubated for 20 hrs with 4×10 7 wt B31-A3 (black bars), B31-4 (gray bars), A3-M9 lp36− (white bars) or A3-M9 lp36−/lp36+ (cross-hatched bar)(MOI = 10). Protein concentrations of human IFN-α ( A ), IFN-λ1 ( B ), or IFN-γ ( C ) in cell-free culture supernatants were quantitated by ELISA ( A , B ) or cytometric bead array ( C ). The results are shown as the mean ± SD of values from three to five blood donors assessed in two or three independent experiments. Statistics were obtained using a non-parametric Mann-Whitney U-test.

    Journal: PLoS ONE

    Article Title: Induction of Type I and Type III Interferons by Borrelia burgdorferi Correlates with Pathogenesis and Requires Linear Plasmid 36

    doi: 10.1371/journal.pone.0100174

    Figure Lengend Snippet: The presence of lp36 is required for IFN-α and IFN-λ induction. Human PBMCs (4×10 6 ) were co-incubated for 20 hrs with 4×10 7 wt B31-A3 (black bars), B31-4 (gray bars), A3-M9 lp36− (white bars) or A3-M9 lp36−/lp36+ (cross-hatched bar)(MOI = 10). Protein concentrations of human IFN-α ( A ), IFN-λ1 ( B ), or IFN-γ ( C ) in cell-free culture supernatants were quantitated by ELISA ( A , B ) or cytometric bead array ( C ). The results are shown as the mean ± SD of values from three to five blood donors assessed in two or three independent experiments. Statistics were obtained using a non-parametric Mann-Whitney U-test.

    Article Snippet: Total numbers of GFP+ PBMCs were determined by flow cytometric analysis of 100,000 to 300,000 events using a MACSQuant Analyzer and MACSQuantify software (Miltenyi Biotec).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Analysis of the expression of Toll-like receptor 2 (TLR2) by flow cytometry in natural killer (NK) cell CD56 bright and CD56 dim subsets . ( A ) Representative flow cytometry histogram of surface expression of TLR2 on CD56 bright and CD56 dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-TLR2. ( B ) Surface TLR2 expression shown as median and interquartile range in all three studied groups. ( C ) Representative flow cytometry histogram of intracellular expression of TLR2 for the same samples represented in ( A) . Black line: isotype control; colored line: anti-TLR2. ( D ) Intracellular TLR2 expression shown as median and interquartile range in all three studied groups. ** P

    Journal: Critical Care

    Article Title: Toll-like receptors expression and interferon-? production by NK cells in human sepsis

    doi: 10.1186/cc11838

    Figure Lengend Snippet: Analysis of the expression of Toll-like receptor 2 (TLR2) by flow cytometry in natural killer (NK) cell CD56 bright and CD56 dim subsets . ( A ) Representative flow cytometry histogram of surface expression of TLR2 on CD56 bright and CD56 dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-TLR2. ( B ) Surface TLR2 expression shown as median and interquartile range in all three studied groups. ( C ) Representative flow cytometry histogram of intracellular expression of TLR2 for the same samples represented in ( A) . Black line: isotype control; colored line: anti-TLR2. ( D ) Intracellular TLR2 expression shown as median and interquartile range in all three studied groups. ** P

    Article Snippet: All flow cytometry data were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using the MACSQuantify software.

    Techniques: Expressing, Flow Cytometry, Cytometry

    Flow cytometry analysis of IFN-γ secretion in whole blood by CD56 bright and CD56 dim natural killer (NK) cell subsets after overnight ex vivo stimulation . ( A ) IL-15 + IL-18; ( B ) IL-15 + IL-18 + LPS; ( C ) IL-15 + IL-18 + CpG-DNA; ( D ) IL-15 + IL-18 + heat-killed S. aureus (HKSA). Median and interquartile range are shown for each group. * P

    Journal: Critical Care

    Article Title: Toll-like receptors expression and interferon-? production by NK cells in human sepsis

    doi: 10.1186/cc11838

    Figure Lengend Snippet: Flow cytometry analysis of IFN-γ secretion in whole blood by CD56 bright and CD56 dim natural killer (NK) cell subsets after overnight ex vivo stimulation . ( A ) IL-15 + IL-18; ( B ) IL-15 + IL-18 + LPS; ( C ) IL-15 + IL-18 + CpG-DNA; ( D ) IL-15 + IL-18 + heat-killed S. aureus (HKSA). Median and interquartile range are shown for each group. * P

    Article Snippet: All flow cytometry data were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using the MACSQuantify software.

    Techniques: Flow Cytometry, Cytometry, Ex Vivo

    Analysis of NK cells CD56 bright and CD56 dim subsets in sepsis and systemic inflammatory response syndrome (SIRS) patients . ( A ) Representative chart of analysis of natural killer (NK) cells in whole blood by flow cytometry. ( B ) Cell counts for total lymphocytes and NK cell subsets (CD56 bright and CD56 dim ) in healthy volunteers, SIRS, and sepsis patients. ( C ) Percent of NK subsets among lymphocytes in healthy volunteers, SIRS, and sepsis patients. Data are shown as median and interquartile range. ** P

    Journal: Critical Care

    Article Title: Toll-like receptors expression and interferon-? production by NK cells in human sepsis

    doi: 10.1186/cc11838

    Figure Lengend Snippet: Analysis of NK cells CD56 bright and CD56 dim subsets in sepsis and systemic inflammatory response syndrome (SIRS) patients . ( A ) Representative chart of analysis of natural killer (NK) cells in whole blood by flow cytometry. ( B ) Cell counts for total lymphocytes and NK cell subsets (CD56 bright and CD56 dim ) in healthy volunteers, SIRS, and sepsis patients. ( C ) Percent of NK subsets among lymphocytes in healthy volunteers, SIRS, and sepsis patients. Data are shown as median and interquartile range. ** P

    Article Snippet: All flow cytometry data were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using the MACSQuantify software.

    Techniques: Flow Cytometry, Cytometry

    Flow cytometry analysis of Toll-like receptor 4 (TLR4) expression in natural killer (NK) cell CD56 bright and CD56 dim subsets . ( A ) Representative flow cytometry histogram of surface expression of TLR4 on CD56 bright and CD56 dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-TLR4. ( B ) Surface TLR4 expression shown as median and interquartile range in all three studied groups. ( C ) Representative flow cytometry histogram of intracellular expression of TLR4 for the same samples represented in ( A ). Black line: isotype control; colored line: anti-TLR4. ( D ) Intracellular TLR4 expression shown as median and interquartile range in all three groups. * P

    Journal: Critical Care

    Article Title: Toll-like receptors expression and interferon-? production by NK cells in human sepsis

    doi: 10.1186/cc11838

    Figure Lengend Snippet: Flow cytometry analysis of Toll-like receptor 4 (TLR4) expression in natural killer (NK) cell CD56 bright and CD56 dim subsets . ( A ) Representative flow cytometry histogram of surface expression of TLR4 on CD56 bright and CD56 dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-TLR4. ( B ) Surface TLR4 expression shown as median and interquartile range in all three studied groups. ( C ) Representative flow cytometry histogram of intracellular expression of TLR4 for the same samples represented in ( A ). Black line: isotype control; colored line: anti-TLR4. ( D ) Intracellular TLR4 expression shown as median and interquartile range in all three groups. * P

    Article Snippet: All flow cytometry data were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using the MACSQuantify software.

    Techniques: Flow Cytometry, Cytometry, Expressing

    Expression of CD69 on natural killer (NK) cell CD56 bright and CD56 dim subsets on freshly isolated cells or after in vitro cultures . ( A ) Representative flow cytometry histograms of surface basal expression of CD69 on CD56 bright and CD56 dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-CD69. ( B ) CD69 expression in all three groups for freshly isolated NK cells (basal) or after overnight culture in the presence of IL-15 + IL-18; data are shown as median and interquartile range. CD69 expression expressed after overnight culture in the presence of IL-15 + IL-18 and lipopolysaccharide (LPS) ( C ), CpG ( D ) and heat-killed S. aureus (HKSA) ( E ); data are expressed as median and interquartile range. * P

    Journal: Critical Care

    Article Title: Toll-like receptors expression and interferon-? production by NK cells in human sepsis

    doi: 10.1186/cc11838

    Figure Lengend Snippet: Expression of CD69 on natural killer (NK) cell CD56 bright and CD56 dim subsets on freshly isolated cells or after in vitro cultures . ( A ) Representative flow cytometry histograms of surface basal expression of CD69 on CD56 bright and CD56 dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-CD69. ( B ) CD69 expression in all three groups for freshly isolated NK cells (basal) or after overnight culture in the presence of IL-15 + IL-18; data are shown as median and interquartile range. CD69 expression expressed after overnight culture in the presence of IL-15 + IL-18 and lipopolysaccharide (LPS) ( C ), CpG ( D ) and heat-killed S. aureus (HKSA) ( E ); data are expressed as median and interquartile range. * P

    Article Snippet: All flow cytometry data were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using the MACSQuantify software.

    Techniques: Expressing, Isolation, In Vitro, Flow Cytometry, Cytometry