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Becton Dickinson flow cytometry calibur
Flow Cytometry Calibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flow cytometry calibur/product/Becton Dickinson
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flow cytometry calibur - by Bioz Stars, 2020-08
88/100 stars

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Flow Cytometry:

Article Title: Cross talk between NADPH oxidase and autophagy in pulmonary artery endothelial cells with intrauterine persistent pulmonary hypertension
Article Snippet: .. The stained cells were then fixed with another 400 μl binding buffer and analyzed on a fluorescence-activated cell sorting Calibur (Becton Dickinson, San Jose, CA) flow cytometer using CellQuest Pro software. .. ROS production was evaluated by DHE fluorescence as we described previously ( , ).

Article Title: RNAe: an effective method for targeted protein translation enhancement by artificial non-coding RNA with SINEB2 repeat
Article Snippet: .. Green fluorescent protein (GFP) fluorescence was measured using a BD Flow Cytometry Calibur (BD Biosciences, USA) using the 488nm laser. .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was isolated from treated cells using the miRspin mRNA Isolation Kit (Tiangen, China).

Article Title: MicroRNA-212 inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting FOXA1
Article Snippet: .. Flow cytometric analysis was carried out with fluorescence activated cell sorting Calibur (Becton Dickinson, San Jose, CA, USA) and Cell Quest software (Becton Dickinson). .. An Annexin-V-FLUOS Staining Kit (Hoffman-La Roche Ltd.,) was used to analyze the level of apoptosis, following the manufacturer’s instruction.

Article Title: MicroRNA-33a promotes cell proliferation and inhibits apoptosis by targeting PPARα in human hepatocellular carcinoma
Article Snippet: .. Flow cytometric analysis was conducted using fluorescence-activated cell sorting Calibur (BD Biosciences, San Jose, CA, USA) and Cell Quest Pro v.4.0.2 software (BD Biosciences). .. A dual luciferase reporter assay was performed to determine whether PPARα was a downstream target gene of miR-33a in HCC cells.

Article Title: Crohn's Disease Patients Have More IgG-Binding Fecal Bacteria than Controls
Article Snippet: .. Finally, the bacterial pellet was suspended in 500 μl PBS, mixed with 20 μl propidium iodine (PI) (1 μg/ml; Sigma-Aldrich, Saint Louis, MO), stored on ice in the dark, and analyzed within 1 h. Flow cytometry analysis of the fecal bacteria was performed with a flow cytometry Calibur (Becton Dickinson). ..

Article Title: Immunomodulatory Gene Therapy Prevents Antibody Formation and Lethal Hypersensitivity Reactions in Murine Pompe Disease
Article Snippet: .. The freshly isolated cells were then stained for 30 minutes with CD25-FITC, CD3-PerCP, and CD4-APC and analyzed by multiparameter flow cytometry using a fluorescence-activated cell sorting Calibur (BD Biosciences). ..

Fluorescence:

Article Title: Tumor Cell–Accelerated Senescence Is Associated With DNA-PKcs Status and Telomere Dysfunction Induced by Radiation
Article Snippet: .. The samples were detected with fluorescence-activated cell sorting Calibur (Becton, Dickinson and Company, CA, USA). ..

Article Title: Use of atorvastatin as an anti-inflammatory treatment in Crohn's disease
Article Snippet: .. FITC-conjugated anti-CX3 CR1 was from MBL International (Woburn, MA, USA); APC-conjugated anti-CD56 from MACS (Miltenyi Biotec, Bergisch Gladbach, Germany); PE-conjugated anti-CD16, Alexa Fluor 647 anti-CCR2, PerCP-conjugated anti-CD14, mouse IgG1 and IgG2b and BD FACS lysing solution were all obtained from BD Pharmingen (Stockholm, Sweden); Fluorochrome-conjugated isotype-matched control antibodies against rat IgG2b , eBioscience (San Diego, CA, USA); fluorescence-activated cell sorting Calibur and CellQuest, BD Biosciences (Stockholm, Sweden); WinList, Verity Software House Inc. (Topsham, ME, USA). .. Results are expressed as medians with IQR (q1–q3).

Article Title: RNAe: an effective method for targeted protein translation enhancement by artificial non-coding RNA with SINEB2 repeat
Article Snippet: .. Green fluorescent protein (GFP) fluorescence was measured using a BD Flow Cytometry Calibur (BD Biosciences, USA) using the 488nm laser. .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was isolated from treated cells using the miRspin mRNA Isolation Kit (Tiangen, China).

Article Title: MicroRNA-212 inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting FOXA1
Article Snippet: .. Flow cytometric analysis was carried out with fluorescence activated cell sorting Calibur (Becton Dickinson, San Jose, CA, USA) and Cell Quest software (Becton Dickinson). .. An Annexin-V-FLUOS Staining Kit (Hoffman-La Roche Ltd.,) was used to analyze the level of apoptosis, following the manufacturer’s instruction.

Article Title: MicroRNA-33a promotes cell proliferation and inhibits apoptosis by targeting PPARα in human hepatocellular carcinoma
Article Snippet: .. Flow cytometric analysis was conducted using fluorescence-activated cell sorting Calibur (BD Biosciences, San Jose, CA, USA) and Cell Quest Pro v.4.0.2 software (BD Biosciences). .. A dual luciferase reporter assay was performed to determine whether PPARα was a downstream target gene of miR-33a in HCC cells.

Article Title: Immunomodulatory Gene Therapy Prevents Antibody Formation and Lethal Hypersensitivity Reactions in Murine Pompe Disease
Article Snippet: .. The freshly isolated cells were then stained for 30 minutes with CD25-FITC, CD3-PerCP, and CD4-APC and analyzed by multiparameter flow cytometry using a fluorescence-activated cell sorting Calibur (BD Biosciences). ..

Isolation:

Article Title: Immunomodulatory Gene Therapy Prevents Antibody Formation and Lethal Hypersensitivity Reactions in Murine Pompe Disease
Article Snippet: .. The freshly isolated cells were then stained for 30 minutes with CD25-FITC, CD3-PerCP, and CD4-APC and analyzed by multiparameter flow cytometry using a fluorescence-activated cell sorting Calibur (BD Biosciences). ..

Cytometry:

Article Title: Cross talk between NADPH oxidase and autophagy in pulmonary artery endothelial cells with intrauterine persistent pulmonary hypertension
Article Snippet: .. The stained cells were then fixed with another 400 μl binding buffer and analyzed on a fluorescence-activated cell sorting Calibur (Becton Dickinson, San Jose, CA) flow cytometer using CellQuest Pro software. .. ROS production was evaluated by DHE fluorescence as we described previously ( , ).

Article Title: RNAe: an effective method for targeted protein translation enhancement by artificial non-coding RNA with SINEB2 repeat
Article Snippet: .. Green fluorescent protein (GFP) fluorescence was measured using a BD Flow Cytometry Calibur (BD Biosciences, USA) using the 488nm laser. .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was isolated from treated cells using the miRspin mRNA Isolation Kit (Tiangen, China).

Article Title: Crohn's Disease Patients Have More IgG-Binding Fecal Bacteria than Controls
Article Snippet: .. Finally, the bacterial pellet was suspended in 500 μl PBS, mixed with 20 μl propidium iodine (PI) (1 μg/ml; Sigma-Aldrich, Saint Louis, MO), stored on ice in the dark, and analyzed within 1 h. Flow cytometry analysis of the fecal bacteria was performed with a flow cytometry Calibur (Becton Dickinson). ..

Article Title: Immunomodulatory Gene Therapy Prevents Antibody Formation and Lethal Hypersensitivity Reactions in Murine Pompe Disease
Article Snippet: .. The freshly isolated cells were then stained for 30 minutes with CD25-FITC, CD3-PerCP, and CD4-APC and analyzed by multiparameter flow cytometry using a fluorescence-activated cell sorting Calibur (BD Biosciences). ..

Staining:

Article Title: Cross talk between NADPH oxidase and autophagy in pulmonary artery endothelial cells with intrauterine persistent pulmonary hypertension
Article Snippet: .. The stained cells were then fixed with another 400 μl binding buffer and analyzed on a fluorescence-activated cell sorting Calibur (Becton Dickinson, San Jose, CA) flow cytometer using CellQuest Pro software. .. ROS production was evaluated by DHE fluorescence as we described previously ( , ).

Article Title: Immunomodulatory Gene Therapy Prevents Antibody Formation and Lethal Hypersensitivity Reactions in Murine Pompe Disease
Article Snippet: .. The freshly isolated cells were then stained for 30 minutes with CD25-FITC, CD3-PerCP, and CD4-APC and analyzed by multiparameter flow cytometry using a fluorescence-activated cell sorting Calibur (BD Biosciences). ..

FACS:

Article Title: Tumor Cell–Accelerated Senescence Is Associated With DNA-PKcs Status and Telomere Dysfunction Induced by Radiation
Article Snippet: .. The samples were detected with fluorescence-activated cell sorting Calibur (Becton, Dickinson and Company, CA, USA). ..

Article Title: Use of atorvastatin as an anti-inflammatory treatment in Crohn's disease
Article Snippet: .. FITC-conjugated anti-CX3 CR1 was from MBL International (Woburn, MA, USA); APC-conjugated anti-CD56 from MACS (Miltenyi Biotec, Bergisch Gladbach, Germany); PE-conjugated anti-CD16, Alexa Fluor 647 anti-CCR2, PerCP-conjugated anti-CD14, mouse IgG1 and IgG2b and BD FACS lysing solution were all obtained from BD Pharmingen (Stockholm, Sweden); Fluorochrome-conjugated isotype-matched control antibodies against rat IgG2b , eBioscience (San Diego, CA, USA); fluorescence-activated cell sorting Calibur and CellQuest, BD Biosciences (Stockholm, Sweden); WinList, Verity Software House Inc. (Topsham, ME, USA). .. Results are expressed as medians with IQR (q1–q3).

Article Title: MicroRNA-212 inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting FOXA1
Article Snippet: .. Flow cytometric analysis was carried out with fluorescence activated cell sorting Calibur (Becton Dickinson, San Jose, CA, USA) and Cell Quest software (Becton Dickinson). .. An Annexin-V-FLUOS Staining Kit (Hoffman-La Roche Ltd.,) was used to analyze the level of apoptosis, following the manufacturer’s instruction.

Article Title: MicroRNA-33a promotes cell proliferation and inhibits apoptosis by targeting PPARα in human hepatocellular carcinoma
Article Snippet: .. Flow cytometric analysis was conducted using fluorescence-activated cell sorting Calibur (BD Biosciences, San Jose, CA, USA) and Cell Quest Pro v.4.0.2 software (BD Biosciences). .. A dual luciferase reporter assay was performed to determine whether PPARα was a downstream target gene of miR-33a in HCC cells.

Article Title: Immunomodulatory Gene Therapy Prevents Antibody Formation and Lethal Hypersensitivity Reactions in Murine Pompe Disease
Article Snippet: .. The freshly isolated cells were then stained for 30 minutes with CD25-FITC, CD3-PerCP, and CD4-APC and analyzed by multiparameter flow cytometry using a fluorescence-activated cell sorting Calibur (BD Biosciences). ..

Binding Assay:

Article Title: Cross talk between NADPH oxidase and autophagy in pulmonary artery endothelial cells with intrauterine persistent pulmonary hypertension
Article Snippet: .. The stained cells were then fixed with another 400 μl binding buffer and analyzed on a fluorescence-activated cell sorting Calibur (Becton Dickinson, San Jose, CA) flow cytometer using CellQuest Pro software. .. ROS production was evaluated by DHE fluorescence as we described previously ( , ).

Magnetic Cell Separation:

Article Title: Use of atorvastatin as an anti-inflammatory treatment in Crohn's disease
Article Snippet: .. FITC-conjugated anti-CX3 CR1 was from MBL International (Woburn, MA, USA); APC-conjugated anti-CD56 from MACS (Miltenyi Biotec, Bergisch Gladbach, Germany); PE-conjugated anti-CD16, Alexa Fluor 647 anti-CCR2, PerCP-conjugated anti-CD14, mouse IgG1 and IgG2b and BD FACS lysing solution were all obtained from BD Pharmingen (Stockholm, Sweden); Fluorochrome-conjugated isotype-matched control antibodies against rat IgG2b , eBioscience (San Diego, CA, USA); fluorescence-activated cell sorting Calibur and CellQuest, BD Biosciences (Stockholm, Sweden); WinList, Verity Software House Inc. (Topsham, ME, USA). .. Results are expressed as medians with IQR (q1–q3).

Software:

Article Title: Cross talk between NADPH oxidase and autophagy in pulmonary artery endothelial cells with intrauterine persistent pulmonary hypertension
Article Snippet: .. The stained cells were then fixed with another 400 μl binding buffer and analyzed on a fluorescence-activated cell sorting Calibur (Becton Dickinson, San Jose, CA) flow cytometer using CellQuest Pro software. .. ROS production was evaluated by DHE fluorescence as we described previously ( , ).

Article Title: MicroRNA-212 inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting FOXA1
Article Snippet: .. Flow cytometric analysis was carried out with fluorescence activated cell sorting Calibur (Becton Dickinson, San Jose, CA, USA) and Cell Quest software (Becton Dickinson). .. An Annexin-V-FLUOS Staining Kit (Hoffman-La Roche Ltd.,) was used to analyze the level of apoptosis, following the manufacturer’s instruction.

Article Title: MicroRNA-33a promotes cell proliferation and inhibits apoptosis by targeting PPARα in human hepatocellular carcinoma
Article Snippet: .. Flow cytometric analysis was conducted using fluorescence-activated cell sorting Calibur (BD Biosciences, San Jose, CA, USA) and Cell Quest Pro v.4.0.2 software (BD Biosciences). .. A dual luciferase reporter assay was performed to determine whether PPARα was a downstream target gene of miR-33a in HCC cells.

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  • 97
    Becton Dickinson facs calibur
    Flow cytometry analysis on reversal of immune-suppression in moDCs in response to L. donovani antigen after stimulation of KMP-11. (a)-(b) Comparison of cytokine production by APCs after costimulation by KMP-11. 1 × 10 6 /mL of either CD14+ MΦ or CD83+ moDCs was stimulated with KMP-11 (10 μ g/mL) and PHA (10 μ g) for 16 h. Harvested cells were consecutively coincubated with Bref-A (1 μ g/mL), surface CD-4 (PE)/CD-83 (PE) antibodies, and cytofix/Perm solution before cytoplasmic staining with FITC for IL-10 and TGF- β and acquired and analyzed on <t>FACS-calibur.</t> Values were expressed in mean ± SEM. Each sample was run in duplicate. *** P
    Facs Calibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 5322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facs calibur/product/Becton Dickinson
    Average 97 stars, based on 5322 article reviews
    Price from $9.99 to $1999.99
    facs calibur - by Bioz Stars, 2020-08
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    94
    Becton Dickinson flow cytometry assay
    Chrysophanol stimulated the necrotic cell death in J5 cells. Cells were exposed to chrysophanol for indicated time periods and the necrotic cells were determined (A and B) by flow <t>cytometry.</t> Data represents mean ± S.D. of three experiments. *p
    Flow Cytometry Assay, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry assay/product/Becton Dickinson
    Average 94 stars, based on 213 article reviews
    Price from $9.99 to $1999.99
    flow cytometry assay - by Bioz Stars, 2020-08
    94/100 stars
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    91
    Becton Dickinson bd facs aria ii
    MRCs support MuSCs in de novo myofibre formation. ( a ) Representative <t>FACS</t> plot of MRCs. Lower limb muscles were dissected and digested to obtain a mononucleated cellular suspension. These cells were marked using cell specific surface antigens as described in the ‘Results’ and in the ‘Methods’ sections and were analysed using FACS. Five populations were isolated: muscle stem cells (MuSCs), hematopoietic cells (HCs), endothelial cells (ECs), fibro-adipogenic progenitor cells (FAPs) and fibroblast-like cells (FLCs). The relative percentages of each cell population are 10%, 25%, 39%, 8% and 18%, respectively ( n =6). ( b ) Quantified results of in vitro bioluminescence generated from cultured bioconstructs containing Luc + MuSCs, either alone (MuSC + /MRC − ) or in combination with Luc − MRCs (MuSC + /MRC + ). Bioconstructs were cultured for three days, and bioluminescence was measured each day ( n =4). ( c ) Representative images of bioluminescence measured from mice 10 days after transplantation of bioconstructs in left TA muscles immediately following VML injury ( d ) Quantified results of non-invasive imaging of transplanted bioconstructs. Bioconstructs with no cells (MuSC − /MRC − ), Luc + MuSCs (MuSC + /MRC − ), or Luc + MuSCs in addition to Luc − MRCs (MuSC + /MRC + ) were transplanted into TA muscles that had received VML injuries. Bioluminescence was measured 10 days following transplantation ( n =4). Data are±s.e.m. For statistical analysis, t -tests were used. * P
    Bd Facs Aria Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bd facs aria ii/product/Becton Dickinson
    Average 91 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    bd facs aria ii - by Bioz Stars, 2020-08
    91/100 stars
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    90
    Becton Dickinson flow cytometer facs calibur
    Representative results of flow cytometric analysis of transfected rat hepatic stellate cells and rat myofibroblasts in comparison to NIH/3T3 cells using FuGENE™6 vehicle. For this experiment rHSCs, rMFBs and NIH/3T3 cells were transfected 2 days after seeding with 2 μg reporter plasmid complexed with 5 μl FuGENE™6. <t>FACS</t> analysis was performed 48 hours after transfection. Cultured cells were trypsinized under standard conditions and flow cytometric measurements were performed immediately after collection of cells. Fluorescence signals were recorded with a flow cytometer <t>FACS-Calibur</t> (Becton Dickinson, Sparks, MD) using a 488 nm excitation and a 530 ± 30 nm emission fluorescence filter for enhanced green fluorescence protein (EGFP) and a 630 ± 11 nm emission fluorescence filter for propidium iodide (PI), respectively. Data were acquired and analyzed with the CellQuest™ software version 3.1 (Becton Dickinson). Histograms of fluorescence intensities in EGFP (A, C, E) and PI (B, D, F) channels are shown for rHSC (A, B) , rMFB (C, D) and reporter cell line NIH/3T3 (E, F) , respectively. To establish background for fluorescence and to set gates for data acquisition, mock-transfected cells (not shown) were used. Mean fluorescence intensity was used to calculate levels of EGFP expression. Cells that took up PI were deemed nonviable. Nontransfected cells did not show fluorescence in EGFP channel.
    Flow Cytometer Facs Calibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometer facs calibur/product/Becton Dickinson
    Average 90 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    flow cytometer facs calibur - by Bioz Stars, 2020-08
    90/100 stars
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    Flow cytometry analysis on reversal of immune-suppression in moDCs in response to L. donovani antigen after stimulation of KMP-11. (a)-(b) Comparison of cytokine production by APCs after costimulation by KMP-11. 1 × 10 6 /mL of either CD14+ MΦ or CD83+ moDCs was stimulated with KMP-11 (10 μ g/mL) and PHA (10 μ g) for 16 h. Harvested cells were consecutively coincubated with Bref-A (1 μ g/mL), surface CD-4 (PE)/CD-83 (PE) antibodies, and cytofix/Perm solution before cytoplasmic staining with FITC for IL-10 and TGF- β and acquired and analyzed on FACS-calibur. Values were expressed in mean ± SEM. Each sample was run in duplicate. *** P

    Journal: BioMed Research International

    Article Title: Immunomodulation in Human Dendritic Cells Leads to Induction of Interferon-Gamma Production by Leishmania donovani Derived KMP-11 Antigen via Activation of NF-κB in Indian Kala-Azar Patients

    doi: 10.1155/2014/947606

    Figure Lengend Snippet: Flow cytometry analysis on reversal of immune-suppression in moDCs in response to L. donovani antigen after stimulation of KMP-11. (a)-(b) Comparison of cytokine production by APCs after costimulation by KMP-11. 1 × 10 6 /mL of either CD14+ MΦ or CD83+ moDCs was stimulated with KMP-11 (10 μ g/mL) and PHA (10 μ g) for 16 h. Harvested cells were consecutively coincubated with Bref-A (1 μ g/mL), surface CD-4 (PE)/CD-83 (PE) antibodies, and cytofix/Perm solution before cytoplasmic staining with FITC for IL-10 and TGF- β and acquired and analyzed on FACS-calibur. Values were expressed in mean ± SEM. Each sample was run in duplicate. *** P

    Article Snippet: Intracytoplasmic cytokine level was detected on FACS-Calibur as previously described.

    Techniques: Flow Cytometry, Cytometry, Staining, FACS

    Differential NF- κ B pattern after stimulation with KMP-11 in APCs of VL patients and healthy controls. The capacity of moDCs and MΦs for the activation of NF- κ B was compared through intracellular staining using fluorescent conjugated anti-NF- κ B antibodies on flow cytometry. Following stimulations (KMP-11 and PHA), cells were harvested and then incubated with PE-anti-p65 NF- κ B antibodies, acquired and analyzed on FACS Calibur. Total amount of NF- κ B was produced by MΦ and moDC stimulated and unstimulated with rKMP-11 evaluated through FACS calibur. Immunoblotting of VL MΦ and VL DCs (insert, Figure 5 ) with NF- κ B antibody shows that KMP-11 triggered significant phosphorylation of a protein migrating at 65 kDa of NF- κ B. There was no significant difference observed between DC-Ctrl and VL-DC of respective groups. * P

    Journal: BioMed Research International

    Article Title: Immunomodulation in Human Dendritic Cells Leads to Induction of Interferon-Gamma Production by Leishmania donovani Derived KMP-11 Antigen via Activation of NF-κB in Indian Kala-Azar Patients

    doi: 10.1155/2014/947606

    Figure Lengend Snippet: Differential NF- κ B pattern after stimulation with KMP-11 in APCs of VL patients and healthy controls. The capacity of moDCs and MΦs for the activation of NF- κ B was compared through intracellular staining using fluorescent conjugated anti-NF- κ B antibodies on flow cytometry. Following stimulations (KMP-11 and PHA), cells were harvested and then incubated with PE-anti-p65 NF- κ B antibodies, acquired and analyzed on FACS Calibur. Total amount of NF- κ B was produced by MΦ and moDC stimulated and unstimulated with rKMP-11 evaluated through FACS calibur. Immunoblotting of VL MΦ and VL DCs (insert, Figure 5 ) with NF- κ B antibody shows that KMP-11 triggered significant phosphorylation of a protein migrating at 65 kDa of NF- κ B. There was no significant difference observed between DC-Ctrl and VL-DC of respective groups. * P

    Article Snippet: Intracytoplasmic cytokine level was detected on FACS-Calibur as previously described.

    Techniques: Activation Assay, Staining, Flow Cytometry, Cytometry, Incubation, FACS, Produced

    (a) IL-12 release by MΦ and moDC after KMP-11 and PHA stimulation. (a) Comparison of cytokine production by APCs after costimulation by KMP-11. 1 × 10 6 /mL of either CD14+ MΦ or CD83+ moDCs was stimulated with KMP-11 (10 μ g/mL) and PHA (10 μ g) for 16 h. Harvested cells were consecutively coincubated with Bref-A (1 μ g/mL), surface CD-4 (PE)/CD-83 (PE) antibodies, and cytofix/Perm solution before cytoplasmic staining with FITC for IL-12 and acquired and analyzed on FACS-calibur. Values were expressed in mean ± SEM. Each sample was run in duplicate. *** P

    Journal: BioMed Research International

    Article Title: Immunomodulation in Human Dendritic Cells Leads to Induction of Interferon-Gamma Production by Leishmania donovani Derived KMP-11 Antigen via Activation of NF-κB in Indian Kala-Azar Patients

    doi: 10.1155/2014/947606

    Figure Lengend Snippet: (a) IL-12 release by MΦ and moDC after KMP-11 and PHA stimulation. (a) Comparison of cytokine production by APCs after costimulation by KMP-11. 1 × 10 6 /mL of either CD14+ MΦ or CD83+ moDCs was stimulated with KMP-11 (10 μ g/mL) and PHA (10 μ g) for 16 h. Harvested cells were consecutively coincubated with Bref-A (1 μ g/mL), surface CD-4 (PE)/CD-83 (PE) antibodies, and cytofix/Perm solution before cytoplasmic staining with FITC for IL-12 and acquired and analyzed on FACS-calibur. Values were expressed in mean ± SEM. Each sample was run in duplicate. *** P

    Article Snippet: Intracytoplasmic cytokine level was detected on FACS-Calibur as previously described.

    Techniques: Staining, FACS

    Cytokine polarization index after exposure of macrophages and moDCs to KMP-11 and subsequent coculture with T cells in VL patients and healthy controls. Cytokine polarization index after exposure of MΦs and moDCs to KMP-11 and subsequent co-culturing with T cells in VL patients. The index was obtained after calculating the ratio of log⁡ e IFN- γ : log⁡ e IL-10, based on FACS Calibur using CellQuest Pro software.

    Journal: BioMed Research International

    Article Title: Immunomodulation in Human Dendritic Cells Leads to Induction of Interferon-Gamma Production by Leishmania donovani Derived KMP-11 Antigen via Activation of NF-κB in Indian Kala-Azar Patients

    doi: 10.1155/2014/947606

    Figure Lengend Snippet: Cytokine polarization index after exposure of macrophages and moDCs to KMP-11 and subsequent coculture with T cells in VL patients and healthy controls. Cytokine polarization index after exposure of MΦs and moDCs to KMP-11 and subsequent co-culturing with T cells in VL patients. The index was obtained after calculating the ratio of log⁡ e IFN- γ : log⁡ e IL-10, based on FACS Calibur using CellQuest Pro software.

    Article Snippet: Intracytoplasmic cytokine level was detected on FACS-Calibur as previously described.

    Techniques: FACS, Software

    Chrysophanol stimulated the necrotic cell death in J5 cells. Cells were exposed to chrysophanol for indicated time periods and the necrotic cells were determined (A and B) by flow cytometry. Data represents mean ± S.D. of three experiments. *p

    Journal: Molecular nutrition & food research

    Article Title: Chrysophanol induces necrosis through the production of ROS and alteration of ATP levels in J5 human liver cancer cells

    doi: 10.1002/mnfr.200900265

    Figure Lengend Snippet: Chrysophanol stimulated the necrotic cell death in J5 cells. Cells were exposed to chrysophanol for indicated time periods and the necrotic cells were determined (A and B) by flow cytometry. Data represents mean ± S.D. of three experiments. *p

    Article Snippet: A phase-contrast microscope was used to determine morphological changes and a flow cytometry assay (Becton Dickinson FACS Calibur) was used to determine cell viability as previously described [ ].

    Techniques: Flow Cytometry, Cytometry

    MRCs support MuSCs in de novo myofibre formation. ( a ) Representative FACS plot of MRCs. Lower limb muscles were dissected and digested to obtain a mononucleated cellular suspension. These cells were marked using cell specific surface antigens as described in the ‘Results’ and in the ‘Methods’ sections and were analysed using FACS. Five populations were isolated: muscle stem cells (MuSCs), hematopoietic cells (HCs), endothelial cells (ECs), fibro-adipogenic progenitor cells (FAPs) and fibroblast-like cells (FLCs). The relative percentages of each cell population are 10%, 25%, 39%, 8% and 18%, respectively ( n =6). ( b ) Quantified results of in vitro bioluminescence generated from cultured bioconstructs containing Luc + MuSCs, either alone (MuSC + /MRC − ) or in combination with Luc − MRCs (MuSC + /MRC + ). Bioconstructs were cultured for three days, and bioluminescence was measured each day ( n =4). ( c ) Representative images of bioluminescence measured from mice 10 days after transplantation of bioconstructs in left TA muscles immediately following VML injury ( d ) Quantified results of non-invasive imaging of transplanted bioconstructs. Bioconstructs with no cells (MuSC − /MRC − ), Luc + MuSCs (MuSC + /MRC − ), or Luc + MuSCs in addition to Luc − MRCs (MuSC + /MRC + ) were transplanted into TA muscles that had received VML injuries. Bioluminescence was measured 10 days following transplantation ( n =4). Data are±s.e.m. For statistical analysis, t -tests were used. * P

    Journal: Nature Communications

    Article Title: Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss

    doi: 10.1038/ncomms15613

    Figure Lengend Snippet: MRCs support MuSCs in de novo myofibre formation. ( a ) Representative FACS plot of MRCs. Lower limb muscles were dissected and digested to obtain a mononucleated cellular suspension. These cells were marked using cell specific surface antigens as described in the ‘Results’ and in the ‘Methods’ sections and were analysed using FACS. Five populations were isolated: muscle stem cells (MuSCs), hematopoietic cells (HCs), endothelial cells (ECs), fibro-adipogenic progenitor cells (FAPs) and fibroblast-like cells (FLCs). The relative percentages of each cell population are 10%, 25%, 39%, 8% and 18%, respectively ( n =6). ( b ) Quantified results of in vitro bioluminescence generated from cultured bioconstructs containing Luc + MuSCs, either alone (MuSC + /MRC − ) or in combination with Luc − MRCs (MuSC + /MRC + ). Bioconstructs were cultured for three days, and bioluminescence was measured each day ( n =4). ( c ) Representative images of bioluminescence measured from mice 10 days after transplantation of bioconstructs in left TA muscles immediately following VML injury ( d ) Quantified results of non-invasive imaging of transplanted bioconstructs. Bioconstructs with no cells (MuSC − /MRC − ), Luc + MuSCs (MuSC + /MRC − ), or Luc + MuSCs in addition to Luc − MRCs (MuSC + /MRC + ) were transplanted into TA muscles that had received VML injuries. Bioluminescence was measured 10 days following transplantation ( n =4). Data are±s.e.m. For statistical analysis, t -tests were used. * P

    Article Snippet: Cell sorting was performed on calibrated BD-FACS Aria II or BD FACSAria III flow cytometers equipped with 488-nm, 633-nm and 405-nm lasers to obtain the MuSC population.

    Techniques: FACS, Isolation, In Vitro, Generated, Cell Culture, Mouse Assay, Transplantation Assay, Imaging

    Representative results of flow cytometric analysis of transfected rat hepatic stellate cells and rat myofibroblasts in comparison to NIH/3T3 cells using FuGENE™6 vehicle. For this experiment rHSCs, rMFBs and NIH/3T3 cells were transfected 2 days after seeding with 2 μg reporter plasmid complexed with 5 μl FuGENE™6. FACS analysis was performed 48 hours after transfection. Cultured cells were trypsinized under standard conditions and flow cytometric measurements were performed immediately after collection of cells. Fluorescence signals were recorded with a flow cytometer FACS-Calibur (Becton Dickinson, Sparks, MD) using a 488 nm excitation and a 530 ± 30 nm emission fluorescence filter for enhanced green fluorescence protein (EGFP) and a 630 ± 11 nm emission fluorescence filter for propidium iodide (PI), respectively. Data were acquired and analyzed with the CellQuest™ software version 3.1 (Becton Dickinson). Histograms of fluorescence intensities in EGFP (A, C, E) and PI (B, D, F) channels are shown for rHSC (A, B) , rMFB (C, D) and reporter cell line NIH/3T3 (E, F) , respectively. To establish background for fluorescence and to set gates for data acquisition, mock-transfected cells (not shown) were used. Mean fluorescence intensity was used to calculate levels of EGFP expression. Cells that took up PI were deemed nonviable. Nontransfected cells did not show fluorescence in EGFP channel.

    Journal: BMC Cell Biology

    Article Title: Comparative evaluation of gene delivery devices in primary cultures of rat hepatic stellate cells and rat myofibroblasts

    doi: 10.1186/1471-2121-1-4

    Figure Lengend Snippet: Representative results of flow cytometric analysis of transfected rat hepatic stellate cells and rat myofibroblasts in comparison to NIH/3T3 cells using FuGENE™6 vehicle. For this experiment rHSCs, rMFBs and NIH/3T3 cells were transfected 2 days after seeding with 2 μg reporter plasmid complexed with 5 μl FuGENE™6. FACS analysis was performed 48 hours after transfection. Cultured cells were trypsinized under standard conditions and flow cytometric measurements were performed immediately after collection of cells. Fluorescence signals were recorded with a flow cytometer FACS-Calibur (Becton Dickinson, Sparks, MD) using a 488 nm excitation and a 530 ± 30 nm emission fluorescence filter for enhanced green fluorescence protein (EGFP) and a 630 ± 11 nm emission fluorescence filter for propidium iodide (PI), respectively. Data were acquired and analyzed with the CellQuest™ software version 3.1 (Becton Dickinson). Histograms of fluorescence intensities in EGFP (A, C, E) and PI (B, D, F) channels are shown for rHSC (A, B) , rMFB (C, D) and reporter cell line NIH/3T3 (E, F) , respectively. To establish background for fluorescence and to set gates for data acquisition, mock-transfected cells (not shown) were used. Mean fluorescence intensity was used to calculate levels of EGFP expression. Cells that took up PI were deemed nonviable. Nontransfected cells did not show fluorescence in EGFP channel.

    Article Snippet: Fluorescence signals were recorded with a flow cytometer FACS-Calibur (Becton Dickinson, Sparks, MD) using a 488 nm excitation and a 530 ± 30 nm emission fluorescence filter for EGFP and a 630 ± 11 nm emission fluorescence filter for PI, respectively.

    Techniques: Flow Cytometry, Transfection, Plasmid Preparation, FACS, Cell Culture, Fluorescence, Cytometry, Software, Expressing