flow cytometry assay  (Beckman Coulter)


Bioz Verified Symbol Beckman Coulter is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Beckman Coulter flow cytometry assay
    Overexpression of IGF-1R reverses miR-503-mediated suppressive effects on LM3 and HepG2 cells. Notes: LM3 and HepG2 cells were transfected with miR-503 mimic with or without IGF-1R plasmid. ( A ) The protein levels of IGF-1R were then examined using Western blot assay. ( B ) MTT assay and ( C ) flow <t>cytometry</t> were conducted to examine the cell proliferation and apoptosis, respectively. ** P
    Flow Cytometry Assay, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry assay/product/Beckman Coulter
    Average 94 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    flow cytometry assay - by Bioz Stars, 2020-05
    94/100 stars

    Images

    1) Product Images from "MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor"

    Article Title: MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S106351

    Overexpression of IGF-1R reverses miR-503-mediated suppressive effects on LM3 and HepG2 cells. Notes: LM3 and HepG2 cells were transfected with miR-503 mimic with or without IGF-1R plasmid. ( A ) The protein levels of IGF-1R were then examined using Western blot assay. ( B ) MTT assay and ( C ) flow cytometry were conducted to examine the cell proliferation and apoptosis, respectively. ** P
    Figure Legend Snippet: Overexpression of IGF-1R reverses miR-503-mediated suppressive effects on LM3 and HepG2 cells. Notes: LM3 and HepG2 cells were transfected with miR-503 mimic with or without IGF-1R plasmid. ( A ) The protein levels of IGF-1R were then examined using Western blot assay. ( B ) MTT assay and ( C ) flow cytometry were conducted to examine the cell proliferation and apoptosis, respectively. ** P

    Techniques Used: Over Expression, Transfection, Plasmid Preparation, Western Blot, MTT Assay, Flow Cytometry, Cytometry

    Upregulation of miR-503 inhibits cell growth and induces apoptosis in LM3 and HepG2 cells. Notes: ( A ) Real-time RT-PCR was performed to determine the relative expression of miR-503 in LM3 and HepG2 cells transfected with scramble miR (miR-NC) and miR-503 mimic, respectively. ( B ) MTT assay and ( C ) flow cytometry were conducted to examine the cell proliferation and apoptosis, respectively. Nontransfected cells were used as control. ** P
    Figure Legend Snippet: Upregulation of miR-503 inhibits cell growth and induces apoptosis in LM3 and HepG2 cells. Notes: ( A ) Real-time RT-PCR was performed to determine the relative expression of miR-503 in LM3 and HepG2 cells transfected with scramble miR (miR-NC) and miR-503 mimic, respectively. ( B ) MTT assay and ( C ) flow cytometry were conducted to examine the cell proliferation and apoptosis, respectively. Nontransfected cells were used as control. ** P

    Techniques Used: Quantitative RT-PCR, Expressing, Transfection, MTT Assay, Flow Cytometry, Cytometry

    2) Product Images from "Ayapana triplinervis Essential Oil and Its Main Component Thymohydroquinone Dimethyl Ether Inhibit Zika Virus at Doses Devoid of Toxicity in Zebrafish"

    Article Title: Ayapana triplinervis Essential Oil and Its Main Component Thymohydroquinone Dimethyl Ether Inhibit Zika Virus at Doses Devoid of Toxicity in Zebrafish

    Journal: Molecules

    doi: 10.3390/molecules24193447

    Thymohydroquinone dimethyl ether interferes with the early stage of ZIKV infection. ( A ) Schematic representation for time-of-drug-addition assay used to characterize anti-ZIKV activity of THQ (125 µg/mL) in A549 cells. Yellow arrows indicate the presence of THQ. ( B ) Results of GFP expression in ZIKV-infected A549 cells, under different conditions shown in panel A, are analyzed by cytometry assay. The results represent the mean ± SD of four independent experiments and are expressed as relative values compared to vehicle. One-way ANOVA and Dunnett’s test were used for statistical analysis (**** p
    Figure Legend Snippet: Thymohydroquinone dimethyl ether interferes with the early stage of ZIKV infection. ( A ) Schematic representation for time-of-drug-addition assay used to characterize anti-ZIKV activity of THQ (125 µg/mL) in A549 cells. Yellow arrows indicate the presence of THQ. ( B ) Results of GFP expression in ZIKV-infected A549 cells, under different conditions shown in panel A, are analyzed by cytometry assay. The results represent the mean ± SD of four independent experiments and are expressed as relative values compared to vehicle. One-way ANOVA and Dunnett’s test were used for statistical analysis (**** p

    Techniques Used: Infection, Activity Assay, Expressing, Cytometry

    3) Product Images from "Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages"

    Article Title: Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.03118

    Myo1F is required to stimulate a pro-inflammatory phenotype in macrophages. (A) iNOS expression was analyzed by RT-PCR in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. *** p = 0.0005. (B) iNOS and pro-IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M0, M1, or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. (C) Expression of CD80 and CD86 was analyzed by flow cytometry in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype and expressed as Mean Florescence Intensity. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. * p = 0.05, ** p = 0.01. (D) NLRP3, Caspase 1, proIL-1β, and IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. Densitometric analyses obtained from those results are shown as graphs. ** p = 0.01. (E) Secretion of IL-1β and IL-6 in supernatants of WT and Myo1F −/− derived BMM was performed by ELISA assay. LPS and IFN-γ/LPS stimulation was carried out for 5 h. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05, ** p = 0.01, *** p = 0.0005. (F) Western blotting for NLRP3, Caspase 1 and IL-1β in J774 cells overexpressing Myo1F or GFP under homeostatic conditions. GAPDH was used as loading control. n = 3. Densitometric analysis obtained from IL-1β is shown as graph. ** p = 0.01. (G) Secretion of IL-1β in supernatants of J774 cells overexpressing Myo1F or GFP was performed by ELISA assay. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05. (H) Quantification of IL-1β release was analyzed in colonic explants of WT and Myo1F deficient mice stimulated with IFN-γ/LPS. Inflammatory stimulus was administered for 5 h. IL-1β was quantified by ELISA. Graphs are derived from three independent experiments. n = 6. Results are given as mean values ± SEM. * p = 0.05.
    Figure Legend Snippet: Myo1F is required to stimulate a pro-inflammatory phenotype in macrophages. (A) iNOS expression was analyzed by RT-PCR in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. *** p = 0.0005. (B) iNOS and pro-IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M0, M1, or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. (C) Expression of CD80 and CD86 was analyzed by flow cytometry in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype and expressed as Mean Florescence Intensity. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. * p = 0.05, ** p = 0.01. (D) NLRP3, Caspase 1, proIL-1β, and IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. Densitometric analyses obtained from those results are shown as graphs. ** p = 0.01. (E) Secretion of IL-1β and IL-6 in supernatants of WT and Myo1F −/− derived BMM was performed by ELISA assay. LPS and IFN-γ/LPS stimulation was carried out for 5 h. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05, ** p = 0.01, *** p = 0.0005. (F) Western blotting for NLRP3, Caspase 1 and IL-1β in J774 cells overexpressing Myo1F or GFP under homeostatic conditions. GAPDH was used as loading control. n = 3. Densitometric analysis obtained from IL-1β is shown as graph. ** p = 0.01. (G) Secretion of IL-1β in supernatants of J774 cells overexpressing Myo1F or GFP was performed by ELISA assay. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05. (H) Quantification of IL-1β release was analyzed in colonic explants of WT and Myo1F deficient mice stimulated with IFN-γ/LPS. Inflammatory stimulus was administered for 5 h. IL-1β was quantified by ELISA. Graphs are derived from three independent experiments. n = 6. Results are given as mean values ± SEM. * p = 0.05.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Western Blot, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Reduced intestinal epithelial damage and enhanced epithelial restitution in the colonic mucosa of Myo1F −/− was observed after colitis induction. (A) Disease Activity Index of WT and Myo1F deficient mice induced to colitis with 2.5% DSS in drinking water. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05. (B) Hematoxylin eosin staining of colonic sections obtained from colitic WT and Myo1F deficient mice. DSS 2.5% was administered in drinking water for 5 days. Bar = 50 μm. Amplification area is shown. Bar = 25 μm. Histological score was determined from H E stain. **** p = 0.0001. (C) Quantification of myeloid cells isolated from colon of WT and Myo1F −/− mice induced to colitis with DSS for 5 days. Purification and quantification were performed by Flow cytometry. All the results are derived from independent experiments carry out by duplicate. (D) Myo1F presence was evaluated by western blotting in colon cell lysates of WT and Myo1F deficient mice induced to colitis with DSS. Treatment was carried out for 5 days. Control animals received only drinking water. GAPDH was used as loading control. n = 5. (E) Myo1F protein levels were analyzed in colonic macrophages isolated from colitic mice as described in materials and methods. GAPDH was used as loading control. n = 3. (F) Cytokine and chemokine secretion was analyzed by using a multiplex assay in colonic explants of WT and Myo1F deficient mice that were induced to colitis for 5 days. Results are given as mean values ± SEM. * p = 0.05. (G) NLRP3, Caspase 1 and IL-1β was evaluated in cell lysates of colonic epithelium obtained from WT or Myo1F deficient mice under control condition or after 5 days of treatment with DSS. GAPDH was used as loading control. n = 5. (H) pAkt 473 , Akt, pSTAT 701 , STAT1, pSTAT3 705 , and STAT3 were analyzed in lysates of colonic tissue from WT and Myo1F deficient mice induced to colitis with DSS. GAPDH was used as loading control. n = 3. (I) Representative image of immunofluorescence staining for F4/80 (green) and pSTAT3 705 (red) in colonic epithelium from WT and Myo1F deficient mice induced to colitis. Nuclei were stained with Dapi (blue). Bar = 20 μm. Amplified areas of the images are shown. Bar = 10 μm. n = 5. Disease activity index (J) and histological changes (K) were analyzed in WT and Myo1F −/− that were induced to recuperation after colitis induction. Hematoxylin eosin staining was carried out as described in materials and methods. Bar = 50 μm. Arrow marks ulcerated areas. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05, **** p = 0.0001. Histological score was determined from H E stain.
    Figure Legend Snippet: Reduced intestinal epithelial damage and enhanced epithelial restitution in the colonic mucosa of Myo1F −/− was observed after colitis induction. (A) Disease Activity Index of WT and Myo1F deficient mice induced to colitis with 2.5% DSS in drinking water. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05. (B) Hematoxylin eosin staining of colonic sections obtained from colitic WT and Myo1F deficient mice. DSS 2.5% was administered in drinking water for 5 days. Bar = 50 μm. Amplification area is shown. Bar = 25 μm. Histological score was determined from H E stain. **** p = 0.0001. (C) Quantification of myeloid cells isolated from colon of WT and Myo1F −/− mice induced to colitis with DSS for 5 days. Purification and quantification were performed by Flow cytometry. All the results are derived from independent experiments carry out by duplicate. (D) Myo1F presence was evaluated by western blotting in colon cell lysates of WT and Myo1F deficient mice induced to colitis with DSS. Treatment was carried out for 5 days. Control animals received only drinking water. GAPDH was used as loading control. n = 5. (E) Myo1F protein levels were analyzed in colonic macrophages isolated from colitic mice as described in materials and methods. GAPDH was used as loading control. n = 3. (F) Cytokine and chemokine secretion was analyzed by using a multiplex assay in colonic explants of WT and Myo1F deficient mice that were induced to colitis for 5 days. Results are given as mean values ± SEM. * p = 0.05. (G) NLRP3, Caspase 1 and IL-1β was evaluated in cell lysates of colonic epithelium obtained from WT or Myo1F deficient mice under control condition or after 5 days of treatment with DSS. GAPDH was used as loading control. n = 5. (H) pAkt 473 , Akt, pSTAT 701 , STAT1, pSTAT3 705 , and STAT3 were analyzed in lysates of colonic tissue from WT and Myo1F deficient mice induced to colitis with DSS. GAPDH was used as loading control. n = 3. (I) Representative image of immunofluorescence staining for F4/80 (green) and pSTAT3 705 (red) in colonic epithelium from WT and Myo1F deficient mice induced to colitis. Nuclei were stained with Dapi (blue). Bar = 20 μm. Amplified areas of the images are shown. Bar = 10 μm. n = 5. Disease activity index (J) and histological changes (K) were analyzed in WT and Myo1F −/− that were induced to recuperation after colitis induction. Hematoxylin eosin staining was carried out as described in materials and methods. Bar = 50 μm. Arrow marks ulcerated areas. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05, **** p = 0.0001. Histological score was determined from H E stain.

    Techniques Used: Activity Assay, Mouse Assay, Derivative Assay, Staining, Amplification, Isolation, Purification, Flow Cytometry, Cytometry, Western Blot, Multiplex Assay, Immunofluorescence

    4) Product Images from "Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy"

    Article Title: Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy

    Journal: Scientific Reports

    doi: 10.1038/srep23393

    The boosted PDT effect of I-ARCs for cancer cell in vitro . Cellular ROS detection after PDT measured by ( a ) confocal microscopy and ( b ) flow cytometry. Due to the oxygen-loaded Hb presence, I-NARCs generated abundant ROS that could produce oxidative damage in the cells. * P
    Figure Legend Snippet: The boosted PDT effect of I-ARCs for cancer cell in vitro . Cellular ROS detection after PDT measured by ( a ) confocal microscopy and ( b ) flow cytometry. Due to the oxygen-loaded Hb presence, I-NARCs generated abundant ROS that could produce oxidative damage in the cells. * P

    Techniques Used: In Vitro, Confocal Microscopy, Flow Cytometry, Cytometry, Generated

    5) Product Images from "Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma"

    Article Title: Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma

    Journal: World Journal of Surgical Oncology

    doi: 10.1186/s12957-018-1371-0

    Effects of IFI7 knockdown on OSCC cells apoptosis. Flow cytometry was performed to measure the apoptosis ratio of TCA8113 cells ( a ) and TSCCA cells ( b ) transfected with negative control, siRNA1, or siRNA2
    Figure Legend Snippet: Effects of IFI7 knockdown on OSCC cells apoptosis. Flow cytometry was performed to measure the apoptosis ratio of TCA8113 cells ( a ) and TSCCA cells ( b ) transfected with negative control, siRNA1, or siRNA2

    Techniques Used: Flow Cytometry, Cytometry, Transfection, Negative Control

    6) Product Images from "pDsRed-EGFPmtag-, an effective dual fluorescent reporter system for cell-based screens of premature termination codon"

    Article Title: pDsRed-EGFPmtag-, an effective dual fluorescent reporter system for cell-based screens of premature termination codon

    Journal: Cytotechnology

    doi: 10.1007/s10616-014-9728-x

    Flow cytometry analysis of pDsRed-EGFPmtag- expressing cells. Cells determined to be expressing both red and green were gated in the untransfected sample. The y axis represents intensity of red fluorescence and the x axis represents the intensity of green
    Figure Legend Snippet: Flow cytometry analysis of pDsRed-EGFPmtag- expressing cells. Cells determined to be expressing both red and green were gated in the untransfected sample. The y axis represents intensity of red fluorescence and the x axis represents the intensity of green

    Techniques Used: Flow Cytometry, Cytometry, Expressing, Fluorescence

    7) Product Images from "Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli"

    Article Title: Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0160367

    Flow cytometry assay for InaK-Ns and InaK-N/ARG1s. Cells containing different vectors labeled with Ddylight649-conjugated antibody against the HA epitope tag were analyzed by flow cytometry. The excitation laser was 638nm, and the emission filter was 660/20 BP. A-M indicated cells containing the pET23a-T empty vector; pET23a-InaK-N vector; pET23a - ssMalE-InaK-N vector; pET23a-ssTorA-InaK-N vector; pET23a-D 6 -InaK-N vector; pET23a-E 6 -InaK-N vector; pET23a-K 6 -InaK-N vector; pET23a-InaK-N/ARG1 vector; pET23a-ssMalE-InaK-N/ARG vector; pET23a-ssTorA-InaK-N/ARG1 vector; pET23a-D 6 -InaK-N/ARG1 vector; pET23a-E 6 -InaK-N/ARG1 vector; and pET23a-K 6 -InaK-N/ARG1 vector, respectively.
    Figure Legend Snippet: Flow cytometry assay for InaK-Ns and InaK-N/ARG1s. Cells containing different vectors labeled with Ddylight649-conjugated antibody against the HA epitope tag were analyzed by flow cytometry. The excitation laser was 638nm, and the emission filter was 660/20 BP. A-M indicated cells containing the pET23a-T empty vector; pET23a-InaK-N vector; pET23a - ssMalE-InaK-N vector; pET23a-ssTorA-InaK-N vector; pET23a-D 6 -InaK-N vector; pET23a-E 6 -InaK-N vector; pET23a-K 6 -InaK-N vector; pET23a-InaK-N/ARG1 vector; pET23a-ssMalE-InaK-N/ARG vector; pET23a-ssTorA-InaK-N/ARG1 vector; pET23a-D 6 -InaK-N/ARG1 vector; pET23a-E 6 -InaK-N/ARG1 vector; and pET23a-K 6 -InaK-N/ARG1 vector, respectively.

    Techniques Used: Flow Cytometry, Cytometry, Labeling, Plasmid Preparation

    8) Product Images from "Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages"

    Article Title: Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.03118

    Myo1F is required to stimulate a pro-inflammatory phenotype in macrophages. (A) iNOS expression was analyzed by RT-PCR in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. *** p = 0.0005. (B) iNOS and pro-IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M0, M1, or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. (C) Expression of CD80 and CD86 was analyzed by flow cytometry in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype and expressed as Mean Florescence Intensity. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. * p = 0.05, ** p = 0.01. (D) NLRP3, Caspase 1, proIL-1β, and IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. Densitometric analyses obtained from those results are shown as graphs. ** p = 0.01. (E) Secretion of IL-1β and IL-6 in supernatants of WT and Myo1F −/− derived BMM was performed by ELISA assay. LPS and IFN-γ/LPS stimulation was carried out for 5 h. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05, ** p = 0.01, *** p = 0.0005. (F) Western blotting for NLRP3, Caspase 1 and IL-1β in J774 cells overexpressing Myo1F or GFP under homeostatic conditions. GAPDH was used as loading control. n = 3. Densitometric analysis obtained from IL-1β is shown as graph. ** p = 0.01. (G) Secretion of IL-1β in supernatants of J774 cells overexpressing Myo1F or GFP was performed by ELISA assay. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05. (H) Quantification of IL-1β release was analyzed in colonic explants of WT and Myo1F deficient mice stimulated with IFN-γ/LPS. Inflammatory stimulus was administered for 5 h. IL-1β was quantified by ELISA. Graphs are derived from three independent experiments. n = 6. Results are given as mean values ± SEM. * p = 0.05.
    Figure Legend Snippet: Myo1F is required to stimulate a pro-inflammatory phenotype in macrophages. (A) iNOS expression was analyzed by RT-PCR in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. *** p = 0.0005. (B) iNOS and pro-IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M0, M1, or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. (C) Expression of CD80 and CD86 was analyzed by flow cytometry in WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 phenotype and expressed as Mean Florescence Intensity. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation. n = 3. Results are given as mean values ± SD. * p = 0.05, ** p = 0.01. (D) NLRP3, Caspase 1, proIL-1β, and IL-1β were analyzed by western blotting cell lysates of WT and Myo1F deficient bone marrow-derived macrophages differentiated into M1 or M2 phenotype. M1 phenotype was induced by IFN-γ/LPS (20 ng/ml; 1 μg/ml) stimulation and M2 was obtained by IL-4 (20 ng/ml) exposition. GAPDH was used as loading control. n = 3. Densitometric analyses obtained from those results are shown as graphs. ** p = 0.01. (E) Secretion of IL-1β and IL-6 in supernatants of WT and Myo1F −/− derived BMM was performed by ELISA assay. LPS and IFN-γ/LPS stimulation was carried out for 5 h. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05, ** p = 0.01, *** p = 0.0005. (F) Western blotting for NLRP3, Caspase 1 and IL-1β in J774 cells overexpressing Myo1F or GFP under homeostatic conditions. GAPDH was used as loading control. n = 3. Densitometric analysis obtained from IL-1β is shown as graph. ** p = 0.01. (G) Secretion of IL-1β in supernatants of J774 cells overexpressing Myo1F or GFP was performed by ELISA assay. Graphs are derived from independent experiments carry out by duplicate. n = 3. Results are given as mean values ± SEM. * p = 0.05. (H) Quantification of IL-1β release was analyzed in colonic explants of WT and Myo1F deficient mice stimulated with IFN-γ/LPS. Inflammatory stimulus was administered for 5 h. IL-1β was quantified by ELISA. Graphs are derived from three independent experiments. n = 6. Results are given as mean values ± SEM. * p = 0.05.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Western Blot, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Reduced intestinal epithelial damage and enhanced epithelial restitution in the colonic mucosa of Myo1F −/− was observed after colitis induction. (A) Disease Activity Index of WT and Myo1F deficient mice induced to colitis with 2.5% DSS in drinking water. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05. (B) Hematoxylin eosin staining of colonic sections obtained from colitic WT and Myo1F deficient mice. DSS 2.5% was administered in drinking water for 5 days. Bar = 50 μm. Amplification area is shown. Bar = 25 μm. Histological score was determined from H E stain. **** p = 0.0001. (C) Quantification of myeloid cells isolated from colon of WT and Myo1F −/− mice induced to colitis with DSS for 5 days. Purification and quantification were performed by Flow cytometry. All the results are derived from independent experiments carry out by duplicate. (D) Myo1F presence was evaluated by western blotting in colon cell lysates of WT and Myo1F deficient mice induced to colitis with DSS. Treatment was carried out for 5 days. Control animals received only drinking water. GAPDH was used as loading control. n = 5. (E) Myo1F protein levels were analyzed in colonic macrophages isolated from colitic mice as described in materials and methods. GAPDH was used as loading control. n = 3. (F) Cytokine and chemokine secretion was analyzed by using a multiplex assay in colonic explants of WT and Myo1F deficient mice that were induced to colitis for 5 days. Results are given as mean values ± SEM. * p = 0.05. (G) NLRP3, Caspase 1 and IL-1β was evaluated in cell lysates of colonic epithelium obtained from WT or Myo1F deficient mice under control condition or after 5 days of treatment with DSS. GAPDH was used as loading control. n = 5. (H) pAkt 473 , Akt, pSTAT 701 , STAT1, pSTAT3 705 , and STAT3 were analyzed in lysates of colonic tissue from WT and Myo1F deficient mice induced to colitis with DSS. GAPDH was used as loading control. n = 3. (I) Representative image of immunofluorescence staining for F4/80 (green) and pSTAT3 705 (red) in colonic epithelium from WT and Myo1F deficient mice induced to colitis. Nuclei were stained with Dapi (blue). Bar = 20 μm. Amplified areas of the images are shown. Bar = 10 μm. n = 5. Disease activity index (J) and histological changes (K) were analyzed in WT and Myo1F −/− that were induced to recuperation after colitis induction. Hematoxylin eosin staining was carried out as described in materials and methods. Bar = 50 μm. Arrow marks ulcerated areas. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05, **** p = 0.0001. Histological score was determined from H E stain.
    Figure Legend Snippet: Reduced intestinal epithelial damage and enhanced epithelial restitution in the colonic mucosa of Myo1F −/− was observed after colitis induction. (A) Disease Activity Index of WT and Myo1F deficient mice induced to colitis with 2.5% DSS in drinking water. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05. (B) Hematoxylin eosin staining of colonic sections obtained from colitic WT and Myo1F deficient mice. DSS 2.5% was administered in drinking water for 5 days. Bar = 50 μm. Amplification area is shown. Bar = 25 μm. Histological score was determined from H E stain. **** p = 0.0001. (C) Quantification of myeloid cells isolated from colon of WT and Myo1F −/− mice induced to colitis with DSS for 5 days. Purification and quantification were performed by Flow cytometry. All the results are derived from independent experiments carry out by duplicate. (D) Myo1F presence was evaluated by western blotting in colon cell lysates of WT and Myo1F deficient mice induced to colitis with DSS. Treatment was carried out for 5 days. Control animals received only drinking water. GAPDH was used as loading control. n = 5. (E) Myo1F protein levels were analyzed in colonic macrophages isolated from colitic mice as described in materials and methods. GAPDH was used as loading control. n = 3. (F) Cytokine and chemokine secretion was analyzed by using a multiplex assay in colonic explants of WT and Myo1F deficient mice that were induced to colitis for 5 days. Results are given as mean values ± SEM. * p = 0.05. (G) NLRP3, Caspase 1 and IL-1β was evaluated in cell lysates of colonic epithelium obtained from WT or Myo1F deficient mice under control condition or after 5 days of treatment with DSS. GAPDH was used as loading control. n = 5. (H) pAkt 473 , Akt, pSTAT 701 , STAT1, pSTAT3 705 , and STAT3 were analyzed in lysates of colonic tissue from WT and Myo1F deficient mice induced to colitis with DSS. GAPDH was used as loading control. n = 3. (I) Representative image of immunofluorescence staining for F4/80 (green) and pSTAT3 705 (red) in colonic epithelium from WT and Myo1F deficient mice induced to colitis. Nuclei were stained with Dapi (blue). Bar = 20 μm. Amplified areas of the images are shown. Bar = 10 μm. n = 5. Disease activity index (J) and histological changes (K) were analyzed in WT and Myo1F −/− that were induced to recuperation after colitis induction. Hematoxylin eosin staining was carried out as described in materials and methods. Bar = 50 μm. Arrow marks ulcerated areas. All the results are derived from independent experiments carry out by duplicate. n = 5 mice per group. * p = 0.05, **** p = 0.0001. Histological score was determined from H E stain.

    Techniques Used: Activity Assay, Mouse Assay, Derivative Assay, Staining, Amplification, Isolation, Purification, Flow Cytometry, Cytometry, Western Blot, Multiplex Assay, Immunofluorescence

    9) Product Images from "MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production"

    Article Title: MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050589

    The inhibited phagocytic efficiency of human neutrophils by blockage of Dectin-1 was compensated by exogenous MBL. A. Abrogation effect of Dectin-1 on human neutrophils by 5 µg/mL blocking mAb was measured by flow cytometry. PE-mouse IgG2b was used as isotype control. B and C. Neutrophils containing intracellular FITC- C. albicans had distinctive green fluorescence, and were easily differentiated from the ones without intracellular FITC- C. albicans . Accordingly, the phagocytic efficiency of neutrophils was measured by flow cytometry assay after stimulation with FITC- C. albicans for 30 and 60 min in the presence of 5 µg/mL Dectin-1 blocking mAb and exogenous MBL at a series of concentrations of 2.5, 5 and 10 µg/mL. D. Bar graph depicted the phagocytic efficiency of human neutrophils at 30 or 60 min after stimulation by FITC- C. albicans in the presence of 5 µg/mL Dectin-1 blocking mAb and exogenous MBL at a series of concentrations of 2.5, 5 and 10 µg/mL. Data were represented as mean ± SE (n = 20). * Significant (
    Figure Legend Snippet: The inhibited phagocytic efficiency of human neutrophils by blockage of Dectin-1 was compensated by exogenous MBL. A. Abrogation effect of Dectin-1 on human neutrophils by 5 µg/mL blocking mAb was measured by flow cytometry. PE-mouse IgG2b was used as isotype control. B and C. Neutrophils containing intracellular FITC- C. albicans had distinctive green fluorescence, and were easily differentiated from the ones without intracellular FITC- C. albicans . Accordingly, the phagocytic efficiency of neutrophils was measured by flow cytometry assay after stimulation with FITC- C. albicans for 30 and 60 min in the presence of 5 µg/mL Dectin-1 blocking mAb and exogenous MBL at a series of concentrations of 2.5, 5 and 10 µg/mL. D. Bar graph depicted the phagocytic efficiency of human neutrophils at 30 or 60 min after stimulation by FITC- C. albicans in the presence of 5 µg/mL Dectin-1 blocking mAb and exogenous MBL at a series of concentrations of 2.5, 5 and 10 µg/mL. Data were represented as mean ± SE (n = 20). * Significant (

    Techniques Used: Blocking Assay, Flow Cytometry, Cytometry, Fluorescence

    MBL - pre-incubated C. albicans stimulated intracellular expression of Dectin-1 in human neutrophils. The expression of neutrophil Dectin-1 was measured by PE-anti-human Dectin-1 mAb using flow cytometry at indicated time points after stimulation by live or HK- C. albicans at a MOI of 10 which was pre-treated with 10 µg/mL MBL. Mouse IgG2b was used as an isotype control. A. C. albicans -stimulated neutrophils were fixed with 1% paraformaldehyde, and the expression of Dectin-1 was measured by flow cytometry. B. Bar graph depicted the expression of Dectin-1 in neutrophils which were fixed with 1% paraformaldehyde at indicated time points after stimulation. C. After C. albicans -stimulated neutrophils were permeabilized and fixed with BD Cytofix/cytoperm solution for measuring intracellular cytokines, the expression of Dectin-1 was measured by flow cytometry. D. Bar graph depicted the expression of Dectin-1 in neutrophils which were permeabilized and fixed with BD Cytofix/cytoperm solution at indicated time points after stimulation. Data were represented as mean ± SE (n = 20). ** Highly significant (
    Figure Legend Snippet: MBL - pre-incubated C. albicans stimulated intracellular expression of Dectin-1 in human neutrophils. The expression of neutrophil Dectin-1 was measured by PE-anti-human Dectin-1 mAb using flow cytometry at indicated time points after stimulation by live or HK- C. albicans at a MOI of 10 which was pre-treated with 10 µg/mL MBL. Mouse IgG2b was used as an isotype control. A. C. albicans -stimulated neutrophils were fixed with 1% paraformaldehyde, and the expression of Dectin-1 was measured by flow cytometry. B. Bar graph depicted the expression of Dectin-1 in neutrophils which were fixed with 1% paraformaldehyde at indicated time points after stimulation. C. After C. albicans -stimulated neutrophils were permeabilized and fixed with BD Cytofix/cytoperm solution for measuring intracellular cytokines, the expression of Dectin-1 was measured by flow cytometry. D. Bar graph depicted the expression of Dectin-1 in neutrophils which were permeabilized and fixed with BD Cytofix/cytoperm solution at indicated time points after stimulation. Data were represented as mean ± SE (n = 20). ** Highly significant (

    Techniques Used: Incubation, Expressing, Flow Cytometry, Cytometry

    Fluorescence intensity of FITC- C. albicans was homogeneously distributed. A. FITC- C. albicans was selected according to side scatter (SSC) and forward scatter (FSC). Fluorescence intensity of FITC- C. albicans was detected by flow cytometry. B. FITC- C. albicans was examined by Laser Confocal microscopy (10×100). The merged image (left below) showed the distribution of FITC on the C. albicans . Scale bar, 5 µm.
    Figure Legend Snippet: Fluorescence intensity of FITC- C. albicans was homogeneously distributed. A. FITC- C. albicans was selected according to side scatter (SSC) and forward scatter (FSC). Fluorescence intensity of FITC- C. albicans was detected by flow cytometry. B. FITC- C. albicans was examined by Laser Confocal microscopy (10×100). The merged image (left below) showed the distribution of FITC on the C. albicans . Scale bar, 5 µm.

    Techniques Used: Fluorescence, Flow Cytometry, Cytometry, Confocal Microscopy

    Abrogation of Dectin-1 partly inhibited ROS production in neutrophils which was stimulated by C. albicans in the presence of MBL. A. Following the pretreatment with 0.1% Tween-20/PBS solution containing 10 µg/mL of MBL and 5 µg/mL of anti-human Dectin-1 blocking mAb or the mouse IgG2b (Isotype control), the maximum value of intracellular ROS in neutrophils stimulated by live C. albicans (MOI = 10) was determined by flow cytometry during 120 min. The neutrophils treated with PBS containing 10 µg/mL of MBL were set as the Tween-20 control. The expression of ROS was represented as mean fluorescence intensity (MFI). B. Bar graph depicted the maximum value of intracellular ROS during 120 min after stimulation by live or HK- C. albicans in the presence of 10 µg/mL of MBL and different dosages of Dectin-1 blocking mAb or the isotype. Data were represented as mean ± SE (n = 20). ** Highly significant (
    Figure Legend Snippet: Abrogation of Dectin-1 partly inhibited ROS production in neutrophils which was stimulated by C. albicans in the presence of MBL. A. Following the pretreatment with 0.1% Tween-20/PBS solution containing 10 µg/mL of MBL and 5 µg/mL of anti-human Dectin-1 blocking mAb or the mouse IgG2b (Isotype control), the maximum value of intracellular ROS in neutrophils stimulated by live C. albicans (MOI = 10) was determined by flow cytometry during 120 min. The neutrophils treated with PBS containing 10 µg/mL of MBL were set as the Tween-20 control. The expression of ROS was represented as mean fluorescence intensity (MFI). B. Bar graph depicted the maximum value of intracellular ROS during 120 min after stimulation by live or HK- C. albicans in the presence of 10 µg/mL of MBL and different dosages of Dectin-1 blocking mAb or the isotype. Data were represented as mean ± SE (n = 20). ** Highly significant (

    Techniques Used: Blocking Assay, Flow Cytometry, Cytometry, Expressing, Fluorescence

    10) Product Images from "Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma"

    Article Title: Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma

    Journal: World Journal of Surgical Oncology

    doi: 10.1186/s12957-018-1371-0

    Effects of IFI7 knockdown on OSCC cells apoptosis. Flow cytometry was performed to measure the apoptosis ratio of TCA8113 cells ( a ) and TSCCA cells ( b ) transfected with negative control, siRNA1, or siRNA2
    Figure Legend Snippet: Effects of IFI7 knockdown on OSCC cells apoptosis. Flow cytometry was performed to measure the apoptosis ratio of TCA8113 cells ( a ) and TSCCA cells ( b ) transfected with negative control, siRNA1, or siRNA2

    Techniques Used: Flow Cytometry, Cytometry, Transfection, Negative Control

    Related Articles

    Flow Cytometry:

    Article Title: Ayapana triplinervis Essential Oil and Its Main Component Thymohydroquinone Dimethyl Ether Inhibit Zika Virus at Doses Devoid of Toxicity in Zebrafish
    Article Snippet: .. Flow Cytometry Assay For cytometry assay, cells were harvested, fixed with 3.7% PFA in PBS for 20 min, washed twice with PBS, and then subjected to a flow cytometric analysis using Cytoflex (Beckman). .. Results were analyzed using cytExpert software.

    Article Title: Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages
    Article Snippet: .. Flow cytometry assay was performed on CytoFLEX cytometer (Beckman-Coulter) and analyzed with the CytExpert software. .. Antibodies and Reagents Primary antibodies were as follows: Myo1F HPA055242 (Sigma-Aldrich), GAPDH/sc-322 (Santa Cruz Biotechnology®), TNF-α #11948, pSTAT1-Tyr701 #9167, pS6 #15967, pAkt-Ser473 #4060, pAkt-Ser308 #4056, Akt1 #2967 from Cell Signaling Technology®, CD86-PE-CY5 #15-0862-82 (eBioscience), and CD80-PE #553769 (BD pharmingen).

    Article Title: Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy
    Article Snippet: .. Cells were also collected to quantify the FL intensity of ROS and ICG by flow cytometry assay (QUANTA SC, Beckman, USA). .. In vitro quantification of ferryl-Hb MCF-7 cells were seeded into 6-well plate (2 × 105 well−1 ), incubated with ARCs (contained 804 μg mL−1 Hb, ARCs were also deoxygenated for comparison), I-ARCs (contained 100 μg mL−1 ICG and 804 μg mL−1 Hb, I-ARCs were also deoxygenated for comparison) for 30 min.

    Article Title: Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli
    Article Snippet: .. For the flow cytometry assay, cells labeled with Dylight649 were analyzed using the Cytoflex cell sorter (Beckman Coulter, USA). ..

    Article Title: Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma
    Article Snippet: .. The sample was then placed on ice and in darkness, and flow cytometry assay (Beckman Coulter) was performed. .. Cell migration assay 1 × 105 cells were resuspended in 100 μL of serum-free medium and added to the upper chamber of Transwell (8-μm pore size; BD Biosciences, San Jose, CA, USA), and 600 μL of complete medium was added to the lower chamber.

    Article Title: pDsRed-EGFPmtag-, an effective dual fluorescent reporter system for cell-based screens of premature termination codon
    Article Snippet: .. Flow cytometry assay was performed on FC500 (Beckman Coulter), and data were analyzed using Expo V2.0 software. .. Statistcal analysis: For each experiment, at least three independent transfection experiments were performed.

    Article Title: MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor
    Article Snippet: .. Then, 300 μL binding buffer was added followed by flow cytometry assay (Accuri C6; Beckman Coulter, Brea, CA, USA). .. Western blotting LM3 and HepG2 cells were solubilized in cold radioimmunoprecipitation assay (RIPA) lysis buffer (Thermo Fisher Scientific) to extract protein, which was separated with 10% SDS-PAGE (Pierce, Rockford, IL, USA) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific).

    Binding Assay:

    Article Title: MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor
    Article Snippet: .. Then, 300 μL binding buffer was added followed by flow cytometry assay (Accuri C6; Beckman Coulter, Brea, CA, USA). .. Western blotting LM3 and HepG2 cells were solubilized in cold radioimmunoprecipitation assay (RIPA) lysis buffer (Thermo Fisher Scientific) to extract protein, which was separated with 10% SDS-PAGE (Pierce, Rockford, IL, USA) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific).

    Cytometry:

    Article Title: Ayapana triplinervis Essential Oil and Its Main Component Thymohydroquinone Dimethyl Ether Inhibit Zika Virus at Doses Devoid of Toxicity in Zebrafish
    Article Snippet: .. Flow Cytometry Assay For cytometry assay, cells were harvested, fixed with 3.7% PFA in PBS for 20 min, washed twice with PBS, and then subjected to a flow cytometric analysis using Cytoflex (Beckman). .. Results were analyzed using cytExpert software.

    Article Title: Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages
    Article Snippet: .. Flow cytometry assay was performed on CytoFLEX cytometer (Beckman-Coulter) and analyzed with the CytExpert software. .. Antibodies and Reagents Primary antibodies were as follows: Myo1F HPA055242 (Sigma-Aldrich), GAPDH/sc-322 (Santa Cruz Biotechnology®), TNF-α #11948, pSTAT1-Tyr701 #9167, pS6 #15967, pAkt-Ser473 #4060, pAkt-Ser308 #4056, Akt1 #2967 from Cell Signaling Technology®, CD86-PE-CY5 #15-0862-82 (eBioscience), and CD80-PE #553769 (BD pharmingen).

    Article Title: Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy
    Article Snippet: .. Cells were also collected to quantify the FL intensity of ROS and ICG by flow cytometry assay (QUANTA SC, Beckman, USA). .. In vitro quantification of ferryl-Hb MCF-7 cells were seeded into 6-well plate (2 × 105 well−1 ), incubated with ARCs (contained 804 μg mL−1 Hb, ARCs were also deoxygenated for comparison), I-ARCs (contained 100 μg mL−1 ICG and 804 μg mL−1 Hb, I-ARCs were also deoxygenated for comparison) for 30 min.

    Article Title: Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli
    Article Snippet: .. For the flow cytometry assay, cells labeled with Dylight649 were analyzed using the Cytoflex cell sorter (Beckman Coulter, USA). ..

    Article Title: Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma
    Article Snippet: .. The sample was then placed on ice and in darkness, and flow cytometry assay (Beckman Coulter) was performed. .. Cell migration assay 1 × 105 cells were resuspended in 100 μL of serum-free medium and added to the upper chamber of Transwell (8-μm pore size; BD Biosciences, San Jose, CA, USA), and 600 μL of complete medium was added to the lower chamber.

    Article Title: pDsRed-EGFPmtag-, an effective dual fluorescent reporter system for cell-based screens of premature termination codon
    Article Snippet: .. Flow cytometry assay was performed on FC500 (Beckman Coulter), and data were analyzed using Expo V2.0 software. .. Statistcal analysis: For each experiment, at least three independent transfection experiments were performed.

    Article Title: MiR-503 inhibits hepatocellular carcinoma cell growth via inhibition of insulin-like growth factor 1 receptor
    Article Snippet: .. Then, 300 μL binding buffer was added followed by flow cytometry assay (Accuri C6; Beckman Coulter, Brea, CA, USA). .. Western blotting LM3 and HepG2 cells were solubilized in cold radioimmunoprecipitation assay (RIPA) lysis buffer (Thermo Fisher Scientific) to extract protein, which was separated with 10% SDS-PAGE (Pierce, Rockford, IL, USA) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific).

    Labeling:

    Article Title: Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli
    Article Snippet: .. For the flow cytometry assay, cells labeled with Dylight649 were analyzed using the Cytoflex cell sorter (Beckman Coulter, USA). ..

    Software:

    Article Title: Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages
    Article Snippet: .. Flow cytometry assay was performed on CytoFLEX cytometer (Beckman-Coulter) and analyzed with the CytExpert software. .. Antibodies and Reagents Primary antibodies were as follows: Myo1F HPA055242 (Sigma-Aldrich), GAPDH/sc-322 (Santa Cruz Biotechnology®), TNF-α #11948, pSTAT1-Tyr701 #9167, pS6 #15967, pAkt-Ser473 #4060, pAkt-Ser308 #4056, Akt1 #2967 from Cell Signaling Technology®, CD86-PE-CY5 #15-0862-82 (eBioscience), and CD80-PE #553769 (BD pharmingen).

    Article Title: pDsRed-EGFPmtag-, an effective dual fluorescent reporter system for cell-based screens of premature termination codon
    Article Snippet: .. Flow cytometry assay was performed on FC500 (Beckman Coulter), and data were analyzed using Expo V2.0 software. .. Statistcal analysis: For each experiment, at least three independent transfection experiments were performed.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Beckman Coulter flow cytometry measurements
    ( A ) Cell viability of HepG2 cells treated with free DOX for 24 h; ( B ) Cell viability within 24 h under different intensity NIR irradiation only for 5 min; ( C ) Cytotoxicities of blank micelles (CBMs), DOX-loading micelles (DCBMs), and the control group with/without laser irradiation (+/−) in HepG2 cells after 24 h treatments using the CCK-8 assay; ( D ) Percentages of cells with different apoptosis analysis stages by flow <t>cytometry.</t> B1: Necrosis; B2: Late apoptotic; B3: Early apoptotic; B4: Viable; a: Control; b: Control + NIR; c: CBM; d: CBM + NIR; e: DCBM; f: DCBM + NIR; ( E ) Apoptosis analyzed by flow cytometry in HepG2 cells at 24 h after various treatments.
    Flow Cytometry Measurements, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry measurements/product/Beckman Coulter
    Average 92 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    flow cytometry measurements - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    99
    Beckman Coulter cytoflex flow cytometer
    Dengue non-structural protein 1 (NS1) nor dengue virus (DENV) induce platelet desialylation. Binding of (A) SNA or (B) MAL-II lectins to washed platelets or (C) the expression of P-selectin after incubation with two concentrations of DENV2 NS1 protein for 4 hrs at 37°C (n = 7 platelet donors). (D) shows expression of platelet P-selectin and (E-G) the binding of SNA, MAL-II, or RCA lectins to washed platelets following incubation with dengue virus type 2 (DENV 2) for 3 hrs at 37°C (n = 8 platelet donors). Purified neuraminidase from C . perfringens (100 mU) and the platelet agonist adenosine diphosphate (ADP) at 125 μM were used as positive controls. Mock infection with supernatant of uninfected C6/36 cells harvested at the same time as DENV2 stocks was used as negative control. Data are shown as geometric mean with 95% confidence interval. Samples depicted in panels A-C were analyzed on a Beckman coulter <t>Cytoflex,</t> and samples in panel D-G on a FC500 flow cytometer, which explains the differences in MFI values. Differences between groups were analyzed using the Mann-Whitney U test, * P
    Cytoflex Flow Cytometer, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytoflex flow cytometer/product/Beckman Coulter
    Average 99 stars, based on 509 article reviews
    Price from $9.99 to $1999.99
    cytoflex flow cytometer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Cell viability of HepG2 cells treated with free DOX for 24 h; ( B ) Cell viability within 24 h under different intensity NIR irradiation only for 5 min; ( C ) Cytotoxicities of blank micelles (CBMs), DOX-loading micelles (DCBMs), and the control group with/without laser irradiation (+/−) in HepG2 cells after 24 h treatments using the CCK-8 assay; ( D ) Percentages of cells with different apoptosis analysis stages by flow cytometry. B1: Necrosis; B2: Late apoptotic; B3: Early apoptotic; B4: Viable; a: Control; b: Control + NIR; c: CBM; d: CBM + NIR; e: DCBM; f: DCBM + NIR; ( E ) Apoptosis analyzed by flow cytometry in HepG2 cells at 24 h after various treatments.

    Journal: Nanomaterials

    Article Title: Biodegradable Micelles for NIR/GSH-Triggered Chemophototherapy of Cancer

    doi: 10.3390/nano9010091

    Figure Lengend Snippet: ( A ) Cell viability of HepG2 cells treated with free DOX for 24 h; ( B ) Cell viability within 24 h under different intensity NIR irradiation only for 5 min; ( C ) Cytotoxicities of blank micelles (CBMs), DOX-loading micelles (DCBMs), and the control group with/without laser irradiation (+/−) in HepG2 cells after 24 h treatments using the CCK-8 assay; ( D ) Percentages of cells with different apoptosis analysis stages by flow cytometry. B1: Necrosis; B2: Late apoptotic; B3: Early apoptotic; B4: Viable; a: Control; b: Control + NIR; c: CBM; d: CBM + NIR; e: DCBM; f: DCBM + NIR; ( E ) Apoptosis analyzed by flow cytometry in HepG2 cells at 24 h after various treatments.

    Article Snippet: Flow cytometry measurements were conducted by living cell suspensions and then analyzed using a flow cytometer (Beckman, CA, USA).

    Techniques: Irradiation, CCK-8 Assay, Flow Cytometry, Cytometry

    ( A ) Confocal laser scanning microscope (CLSM) images of HepG2 cells after incubation with DCBMs for 1 h, 2 h, and 4 h. DOX exhibited red fluorescence, and BPLP segments showed blue fluorescence. The scale bars correspond to 30 μm in all the images. ( B ) Flow cytometry analysis of cellular uptake.

    Journal: Nanomaterials

    Article Title: Biodegradable Micelles for NIR/GSH-Triggered Chemophototherapy of Cancer

    doi: 10.3390/nano9010091

    Figure Lengend Snippet: ( A ) Confocal laser scanning microscope (CLSM) images of HepG2 cells after incubation with DCBMs for 1 h, 2 h, and 4 h. DOX exhibited red fluorescence, and BPLP segments showed blue fluorescence. The scale bars correspond to 30 μm in all the images. ( B ) Flow cytometry analysis of cellular uptake.

    Article Snippet: Flow cytometry measurements were conducted by living cell suspensions and then analyzed using a flow cytometer (Beckman, CA, USA).

    Techniques: Laser-Scanning Microscopy, Confocal Laser Scanning Microscopy, Incubation, Fluorescence, Flow Cytometry, Cytometry

    MiR-21-mediated the anti-apoptotic effects of MSCs to hypoxia-induced BEAS-2B cell injury. Beas-2B cells were transfected with miR-21 mimic (a) or miR-21 inhibitor (b), exposed to CoCl 2 for 12 h and cultured in complete medium for 24 h. Cells were collected for apoptosis assay. A random sequence was used as a control for the miR-21 mimic group. The administration of lip2000 was used as a control for the miR-21 inhibitor group. (c) BEAS-2B cells were labeled with cell trace violet for 20 min, seeded in plates, transfected with miR-21 inhibitor and exposed to CoCl 2 for 12 h, followed by co-culture with or without MSCs for an additional 24 h. Apoptosis of cell trace positive BEAS-2B cells was analyzed using flow cytometry. Data are representative of three separate experiments. Data are shown as the mean ± SD. * p

    Journal: Cell Transplantation

    Article Title: MicroRNA-21 Mediates the Protective Effects of Mesenchymal Stem Cells Derived from iPSCs to Human Bronchial Epithelial Cell Injury Under Hypoxia

    doi: 10.1177/0963689718767159

    Figure Lengend Snippet: MiR-21-mediated the anti-apoptotic effects of MSCs to hypoxia-induced BEAS-2B cell injury. Beas-2B cells were transfected with miR-21 mimic (a) or miR-21 inhibitor (b), exposed to CoCl 2 for 12 h and cultured in complete medium for 24 h. Cells were collected for apoptosis assay. A random sequence was used as a control for the miR-21 mimic group. The administration of lip2000 was used as a control for the miR-21 inhibitor group. (c) BEAS-2B cells were labeled with cell trace violet for 20 min, seeded in plates, transfected with miR-21 inhibitor and exposed to CoCl 2 for 12 h, followed by co-culture with or without MSCs for an additional 24 h. Apoptosis of cell trace positive BEAS-2B cells was analyzed using flow cytometry. Data are representative of three separate experiments. Data are shown as the mean ± SD. * p

    Article Snippet: All flow cytometry assays were performed using a Beckman flow cytometer (Beckman-Coulter, Miami, FL, USA), and the data were analyzed using Kaluza software (Beckman-Coulter).

    Techniques: Transfection, Cell Culture, Apoptosis Assay, Sequencing, Labeling, Co-Culture Assay, Flow Cytometry, Cytometry

    MiR-21 regulated CoCl 2 -induced ACVR2A expression. BEAS-2B cells were transfected with miR-21 mimic (a and c) or inhibitor (b and d) and cultured in the presence or absence of CoCl 2 for 36 h. (a, b) ACVR2A mRNA expression was detected via qRT-PCR. (c–d): Percentage of ACVR2A-positive BEAS-2B cells was detected using flow cytometry. Data are shown as the mean ± SD from three independent experiments. (b) BEAS-2B cells were treated with MSCs. * p

    Journal: Cell Transplantation

    Article Title: MicroRNA-21 Mediates the Protective Effects of Mesenchymal Stem Cells Derived from iPSCs to Human Bronchial Epithelial Cell Injury Under Hypoxia

    doi: 10.1177/0963689718767159

    Figure Lengend Snippet: MiR-21 regulated CoCl 2 -induced ACVR2A expression. BEAS-2B cells were transfected with miR-21 mimic (a and c) or inhibitor (b and d) and cultured in the presence or absence of CoCl 2 for 36 h. (a, b) ACVR2A mRNA expression was detected via qRT-PCR. (c–d): Percentage of ACVR2A-positive BEAS-2B cells was detected using flow cytometry. Data are shown as the mean ± SD from three independent experiments. (b) BEAS-2B cells were treated with MSCs. * p

    Article Snippet: All flow cytometry assays were performed using a Beckman flow cytometer (Beckman-Coulter, Miami, FL, USA), and the data were analyzed using Kaluza software (Beckman-Coulter).

    Techniques: Expressing, Transfection, Cell Culture, Quantitative RT-PCR, Flow Cytometry, Cytometry

    Co-culture with MSCs attenuated hypoxia-induced apoptosis. (a) BEAS-2B cells were cultured with different concentrations of CoCl 2 (0, 400, 600, and 800 μM, respectively) for 12 h and subsequently cultured in complete medium for 24 h. Apoptosis was examined using flow cytometry. (b–c) BEAS-2B cells (1×10 5 /well) were labeled with cell trace violet, seeded in a six-well plate and treated with 800 μM CoCl 2 for 12 h after adherence. BEAS-2B cells were further co-cultured with MSCs with different concentrations for 24 h. BEAS-2B cells were analyzed via an apoptosis assay (b) or sorted for Western blot (c). The normal group was not cultured with CoCl 2 or MSCs. (d) BEAS-2B were cultured with 800 μM CoCl 2 and co-cultured with MSCs for 24 h using transwell. Data are representative of three separate experiments. * p

    Journal: Cell Transplantation

    Article Title: MicroRNA-21 Mediates the Protective Effects of Mesenchymal Stem Cells Derived from iPSCs to Human Bronchial Epithelial Cell Injury Under Hypoxia

    doi: 10.1177/0963689718767159

    Figure Lengend Snippet: Co-culture with MSCs attenuated hypoxia-induced apoptosis. (a) BEAS-2B cells were cultured with different concentrations of CoCl 2 (0, 400, 600, and 800 μM, respectively) for 12 h and subsequently cultured in complete medium for 24 h. Apoptosis was examined using flow cytometry. (b–c) BEAS-2B cells (1×10 5 /well) were labeled with cell trace violet, seeded in a six-well plate and treated with 800 μM CoCl 2 for 12 h after adherence. BEAS-2B cells were further co-cultured with MSCs with different concentrations for 24 h. BEAS-2B cells were analyzed via an apoptosis assay (b) or sorted for Western blot (c). The normal group was not cultured with CoCl 2 or MSCs. (d) BEAS-2B were cultured with 800 μM CoCl 2 and co-cultured with MSCs for 24 h using transwell. Data are representative of three separate experiments. * p

    Article Snippet: All flow cytometry assays were performed using a Beckman flow cytometer (Beckman-Coulter, Miami, FL, USA), and the data were analyzed using Kaluza software (Beckman-Coulter).

    Techniques: Co-Culture Assay, Cell Culture, Flow Cytometry, Cytometry, Labeling, Apoptosis Assay, Western Blot

    ACVR2A expression increased in CoCl2 induced BEAS-2B cells. (a) Schematic indicates the interaction sites for human miR-21, which is harbored in the 3’UTR of human ACVR2A. (b) BEAS-2B cells were treated with 400 μM CoCl 2 for 0, 12 h, 24 h and 36 h. The relative expression of ACVR2A mRNA was examined via qRT-PCR. (c) BEAS-2B cells were treated with 400 μM CoCl 2 for 36 h, and the ACVR2A protein level was examined using flow cytometry. (d) BEAS-2B cells were treated with 400 μM CoCl 2 for 12 h and subsequently co-cultured with or without MSCs for 36 h. BEAS-2B cells were harvested and isolated by cell sorting for PCR assay. Data are shown as the mean ± SD from three independent experiments. *** p

    Journal: Cell Transplantation

    Article Title: MicroRNA-21 Mediates the Protective Effects of Mesenchymal Stem Cells Derived from iPSCs to Human Bronchial Epithelial Cell Injury Under Hypoxia

    doi: 10.1177/0963689718767159

    Figure Lengend Snippet: ACVR2A expression increased in CoCl2 induced BEAS-2B cells. (a) Schematic indicates the interaction sites for human miR-21, which is harbored in the 3’UTR of human ACVR2A. (b) BEAS-2B cells were treated with 400 μM CoCl 2 for 0, 12 h, 24 h and 36 h. The relative expression of ACVR2A mRNA was examined via qRT-PCR. (c) BEAS-2B cells were treated with 400 μM CoCl 2 for 36 h, and the ACVR2A protein level was examined using flow cytometry. (d) BEAS-2B cells were treated with 400 μM CoCl 2 for 12 h and subsequently co-cultured with or without MSCs for 36 h. BEAS-2B cells were harvested and isolated by cell sorting for PCR assay. Data are shown as the mean ± SD from three independent experiments. *** p

    Article Snippet: All flow cytometry assays were performed using a Beckman flow cytometer (Beckman-Coulter, Miami, FL, USA), and the data were analyzed using Kaluza software (Beckman-Coulter).

    Techniques: Expressing, Quantitative RT-PCR, Flow Cytometry, Cytometry, Cell Culture, Isolation, FACS, Polymerase Chain Reaction

    The FDAA probes used in this study and their labeling of gut microbiota in vivo. a Structures of TADA, TADA-amide, and TADA-ester. b Confocal fluorescence images of gut microbes from mice administered with TADA-amide. DIC, differential interference contrast. Scale bar, 10 μm. c Statistical analysis of the labeling coverage for gut microbes labeled with TADA, TADA-amide, and TADA-ester. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of TADA-amide-labeled gut microbiota with different labeling time. NC negative control; 1 × 4 h, one gavage and microbiotas collected 4 h later; 2 × 3 h, two gavages with a 3 h interval, and microbiotas collected 3 h after the second gavage. e Confocal fluorescence imaging of the transplanted gut microbiota (green) on the tissue sections of the recipient mouse’s large intestine (L.I.) and small intestine (S.I.). Hoechst 33342 (blue) was used for nuclear counterstain. In b , e , representative results from three independent experiments are shown. Scale bar, 50 μm

    Journal: Nature Communications

    Article Title: Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes

    doi: 10.1038/s41467-019-09267-x

    Figure Lengend Snippet: The FDAA probes used in this study and their labeling of gut microbiota in vivo. a Structures of TADA, TADA-amide, and TADA-ester. b Confocal fluorescence images of gut microbes from mice administered with TADA-amide. DIC, differential interference contrast. Scale bar, 10 μm. c Statistical analysis of the labeling coverage for gut microbes labeled with TADA, TADA-amide, and TADA-ester. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of TADA-amide-labeled gut microbiota with different labeling time. NC negative control; 1 × 4 h, one gavage and microbiotas collected 4 h later; 2 × 3 h, two gavages with a 3 h interval, and microbiotas collected 3 h after the second gavage. e Confocal fluorescence imaging of the transplanted gut microbiota (green) on the tissue sections of the recipient mouse’s large intestine (L.I.) and small intestine (S.I.). Hoechst 33342 (blue) was used for nuclear counterstain. In b , e , representative results from three independent experiments are shown. Scale bar, 50 μm

    Article Snippet: Flow cytometry Flow cytometry analyses and sorting of the FDAA probe labeled microbiota samples were performed on CytoFLex (Beckman Coulter Life Sciences, Indianapolis, IN, USA) and Aria II flow cytometer (BD Biosciences, San Jose, CA, USA).

    Techniques: Labeling, In Vivo, Fluorescence, Mouse Assay, Flow Cytometry, Cytometry, Negative Control, Imaging

    Evaluation of the survival of specific bacteria, and the antibiotic preconditioning effects. a Schematic showing the experimental procedures for assessing the viability of E. coli during transplantation using STAMP. b Flow cytometry analysis of the transplanted E. coli strains K12 (left) and Nissle 1917 (right). The inserted numbers indicate the survival rates. c Statistical analysis of the survival rates of transplanted E. coli K12 and Nissle 1917. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of the STAMP-labeled gut microbiota from recipient mice with preconditioning of vancomycin, polymyxin B, cefotaxime, or metronidazole. The inserted numbers indicate the survival rates. e Statistical analysis of the survival rates of the transplanted microbiotas from recipient mice with antibiotic preconditioning. Mean ± s.d. are presented for n = 3

    Journal: Nature Communications

    Article Title: Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes

    doi: 10.1038/s41467-019-09267-x

    Figure Lengend Snippet: Evaluation of the survival of specific bacteria, and the antibiotic preconditioning effects. a Schematic showing the experimental procedures for assessing the viability of E. coli during transplantation using STAMP. b Flow cytometry analysis of the transplanted E. coli strains K12 (left) and Nissle 1917 (right). The inserted numbers indicate the survival rates. c Statistical analysis of the survival rates of transplanted E. coli K12 and Nissle 1917. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of the STAMP-labeled gut microbiota from recipient mice with preconditioning of vancomycin, polymyxin B, cefotaxime, or metronidazole. The inserted numbers indicate the survival rates. e Statistical analysis of the survival rates of the transplanted microbiotas from recipient mice with antibiotic preconditioning. Mean ± s.d. are presented for n = 3

    Article Snippet: Flow cytometry Flow cytometry analyses and sorting of the FDAA probe labeled microbiota samples were performed on CytoFLex (Beckman Coulter Life Sciences, Indianapolis, IN, USA) and Aria II flow cytometer (BD Biosciences, San Jose, CA, USA).

    Techniques: Transplantation Assay, Flow Cytometry, Cytometry, Labeling, Mouse Assay

    Two-color fluorescence analyses of the transplanted microbiotas. a Two-color fluorescence microscopy analyses of the transplanted microbiota from the recipient mice. Scale bar, 20 μm. b Flow cytometry analysis of the transplanted microbiotas from the recipient mice. The inserted ellipse indicates the survived transplants labeled with both colors, which accounts for 8.6% of the recipient mice’s microbiota. c 16S rDNA sequencing of the bacteria before and after sorting uncovered that several bacterial genera were enriched in the transplantation survivors. d Gut bacteria with two classical dividing patterns were revealed by two-color fluorescence microscopy. Scale bars, 5 μm. Representative data from three independent experiments are shown

    Journal: Nature Communications

    Article Title: Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes

    doi: 10.1038/s41467-019-09267-x

    Figure Lengend Snippet: Two-color fluorescence analyses of the transplanted microbiotas. a Two-color fluorescence microscopy analyses of the transplanted microbiota from the recipient mice. Scale bar, 20 μm. b Flow cytometry analysis of the transplanted microbiotas from the recipient mice. The inserted ellipse indicates the survived transplants labeled with both colors, which accounts for 8.6% of the recipient mice’s microbiota. c 16S rDNA sequencing of the bacteria before and after sorting uncovered that several bacterial genera were enriched in the transplantation survivors. d Gut bacteria with two classical dividing patterns were revealed by two-color fluorescence microscopy. Scale bars, 5 μm. Representative data from three independent experiments are shown

    Article Snippet: Flow cytometry Flow cytometry analyses and sorting of the FDAA probe labeled microbiota samples were performed on CytoFLex (Beckman Coulter Life Sciences, Indianapolis, IN, USA) and Aria II flow cytometer (BD Biosciences, San Jose, CA, USA).

    Techniques: Fluorescence, Microscopy, Mouse Assay, Flow Cytometry, Cytometry, Labeling, Sequencing, Transplantation Assay

    STAMP for assessing the viability of transplanted gut microbiotas. The donor mouse gut microbiota labeled in vivo by the first FDAA probe (TADA-amide) was intragastrically administered to the recipient mouse, which was then given a second FDAA probe (Cy5ADA-amide) by gavage 6 h after the transplantation. The recipient’s gut microbiota was collected, and analyzed by two-color fluorescence microscopy and flow cytometry. The bacteria labeled by both probes were the transplants that survived in the recipient’s gut, bacteria labeled with only TAMRA were probably those did not survive during transplantation, and bacteria labeled only by Cy5 were the recipient’s original gut microbiota

    Journal: Nature Communications

    Article Title: Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes

    doi: 10.1038/s41467-019-09267-x

    Figure Lengend Snippet: STAMP for assessing the viability of transplanted gut microbiotas. The donor mouse gut microbiota labeled in vivo by the first FDAA probe (TADA-amide) was intragastrically administered to the recipient mouse, which was then given a second FDAA probe (Cy5ADA-amide) by gavage 6 h after the transplantation. The recipient’s gut microbiota was collected, and analyzed by two-color fluorescence microscopy and flow cytometry. The bacteria labeled by both probes were the transplants that survived in the recipient’s gut, bacteria labeled with only TAMRA were probably those did not survive during transplantation, and bacteria labeled only by Cy5 were the recipient’s original gut microbiota

    Article Snippet: Flow cytometry Flow cytometry analyses and sorting of the FDAA probe labeled microbiota samples were performed on CytoFLex (Beckman Coulter Life Sciences, Indianapolis, IN, USA) and Aria II flow cytometer (BD Biosciences, San Jose, CA, USA).

    Techniques: Labeling, In Vivo, Transplantation Assay, Fluorescence, Microscopy, Flow Cytometry, Cytometry

    Dengue non-structural protein 1 (NS1) nor dengue virus (DENV) induce platelet desialylation. Binding of (A) SNA or (B) MAL-II lectins to washed platelets or (C) the expression of P-selectin after incubation with two concentrations of DENV2 NS1 protein for 4 hrs at 37°C (n = 7 platelet donors). (D) shows expression of platelet P-selectin and (E-G) the binding of SNA, MAL-II, or RCA lectins to washed platelets following incubation with dengue virus type 2 (DENV 2) for 3 hrs at 37°C (n = 8 platelet donors). Purified neuraminidase from C . perfringens (100 mU) and the platelet agonist adenosine diphosphate (ADP) at 125 μM were used as positive controls. Mock infection with supernatant of uninfected C6/36 cells harvested at the same time as DENV2 stocks was used as negative control. Data are shown as geometric mean with 95% confidence interval. Samples depicted in panels A-C were analyzed on a Beckman coulter Cytoflex, and samples in panel D-G on a FC500 flow cytometer, which explains the differences in MFI values. Differences between groups were analyzed using the Mann-Whitney U test, * P

    Journal: PLoS Pathogens

    Article Title: Desialylation of platelets induced by Von Willebrand Factor is a novel mechanism of platelet clearance in dengue

    doi: 10.1371/journal.ppat.1007500

    Figure Lengend Snippet: Dengue non-structural protein 1 (NS1) nor dengue virus (DENV) induce platelet desialylation. Binding of (A) SNA or (B) MAL-II lectins to washed platelets or (C) the expression of P-selectin after incubation with two concentrations of DENV2 NS1 protein for 4 hrs at 37°C (n = 7 platelet donors). (D) shows expression of platelet P-selectin and (E-G) the binding of SNA, MAL-II, or RCA lectins to washed platelets following incubation with dengue virus type 2 (DENV 2) for 3 hrs at 37°C (n = 8 platelet donors). Purified neuraminidase from C . perfringens (100 mU) and the platelet agonist adenosine diphosphate (ADP) at 125 μM were used as positive controls. Mock infection with supernatant of uninfected C6/36 cells harvested at the same time as DENV2 stocks was used as negative control. Data are shown as geometric mean with 95% confidence interval. Samples depicted in panels A-C were analyzed on a Beckman coulter Cytoflex, and samples in panel D-G on a FC500 flow cytometer, which explains the differences in MFI values. Differences between groups were analyzed using the Mann-Whitney U test, * P

    Article Snippet: Platelets were incubated with saturating amounts of these antibodies for 25 minutes, fixated with 0.2% PFA for 15 minutes and measured on a Cytomics FC500 flow cytometer or a Cytoflex flow cytometer (both Beckman Coulter).

    Techniques: Binding Assay, Expressing, Incubation, Purification, Infection, Negative Control, Flow Cytometry, Cytometry, MANN-WHITNEY