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Bio-Rad flhf cdna
Genetic organization of flhBAFG and fliA genes, and <t>flhF</t> gene expression at 28°C. A . Genetic organization and transcriptional units of flhBAFG and fliA , with arrows showing the direction of transcription. Recognition sites for Eco RI and Stu I are indicated by E and S, respectively. Vertical bars on the map denote the positions and orientation of the Tn 3 - gusA insertion. The two lines with arrowheads beneath the restriction enzyme map indicate the direction and extent of transcription. <t>cDNA</t> synthesized by reverse transcriptase with primers RT1 and RT2 was analyzed by PCR. Thick bars indicate the six expected PCR products, which were verified by electrophoresis on 1.5% agarose gel. B . Photographs of swim assay plates showing the swimming motility of the BGR1 mutant fliA ::Tn 3 - gusA45 and complementation with pBGFA carrying the fliA gene at 28°C and 37°C. C . Expression levels of the flhF gene in the wild-type BGR1, the tofI mutant BGS2, and the qsmR mutant BGS9, and the flhC mutant BGF41 at 28°C, based on qRT-PCR analysis. Vertical lines indicate the standard deviations of three independent experiments.
Flhf Cdna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Quorum Sensing Controls Flagellar Morphogenesis in Burkholderia glumae"

Article Title: Quorum Sensing Controls Flagellar Morphogenesis in Burkholderia glumae

Journal: PLoS ONE

doi: 10.1371/journal.pone.0084831

Genetic organization of flhBAFG and fliA genes, and flhF gene expression at 28°C. A . Genetic organization and transcriptional units of flhBAFG and fliA , with arrows showing the direction of transcription. Recognition sites for Eco RI and Stu I are indicated by E and S, respectively. Vertical bars on the map denote the positions and orientation of the Tn 3 - gusA insertion. The two lines with arrowheads beneath the restriction enzyme map indicate the direction and extent of transcription. cDNA synthesized by reverse transcriptase with primers RT1 and RT2 was analyzed by PCR. Thick bars indicate the six expected PCR products, which were verified by electrophoresis on 1.5% agarose gel. B . Photographs of swim assay plates showing the swimming motility of the BGR1 mutant fliA ::Tn 3 - gusA45 and complementation with pBGFA carrying the fliA gene at 28°C and 37°C. C . Expression levels of the flhF gene in the wild-type BGR1, the tofI mutant BGS2, and the qsmR mutant BGS9, and the flhC mutant BGF41 at 28°C, based on qRT-PCR analysis. Vertical lines indicate the standard deviations of three independent experiments.
Figure Legend Snippet: Genetic organization of flhBAFG and fliA genes, and flhF gene expression at 28°C. A . Genetic organization and transcriptional units of flhBAFG and fliA , with arrows showing the direction of transcription. Recognition sites for Eco RI and Stu I are indicated by E and S, respectively. Vertical bars on the map denote the positions and orientation of the Tn 3 - gusA insertion. The two lines with arrowheads beneath the restriction enzyme map indicate the direction and extent of transcription. cDNA synthesized by reverse transcriptase with primers RT1 and RT2 was analyzed by PCR. Thick bars indicate the six expected PCR products, which were verified by electrophoresis on 1.5% agarose gel. B . Photographs of swim assay plates showing the swimming motility of the BGR1 mutant fliA ::Tn 3 - gusA45 and complementation with pBGFA carrying the fliA gene at 28°C and 37°C. C . Expression levels of the flhF gene in the wild-type BGR1, the tofI mutant BGS2, and the qsmR mutant BGS9, and the flhC mutant BGF41 at 28°C, based on qRT-PCR analysis. Vertical lines indicate the standard deviations of three independent experiments.

Techniques Used: Expressing, Synthesized, Polymerase Chain Reaction, Electrophoresis, Agarose Gel Electrophoresis, Mutagenesis, Quantitative RT-PCR

Related Articles

Quantitative RT-PCR:

Article Title: Quorum Sensing Controls Flagellar Morphogenesis in Burkholderia glumae
Article Snippet: .. For qRT-PCR analysis, the primers of flhC-cDNA, flhF-cDNA, flhC-qRTF, flhC-qRTR, flhF-qRTF, and flhF-qRTR were used ( ). qRT-PCR analysis was performed according to the manufacturer's instructions except using SsoFast™ EvaGreen Supermix (Bio-Rad) with 25 ng of cDNA as template. qRT-PCR was performed using a thermal cycler (Model C1000™; Bio-Rad) under the following conditions: 98°C for 2 min, followed by 35 cycles at 98°C for 20 s, 65°C for 30 s, and 72°C for 30 s. The 16S rRNA gene was used for data normalization. ..

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    Bio-Rad flhf cdna
    Genetic organization of flhBAFG and fliA genes, and <t>flhF</t> gene expression at 28°C. A . Genetic organization and transcriptional units of flhBAFG and fliA , with arrows showing the direction of transcription. Recognition sites for Eco RI and Stu I are indicated by E and S, respectively. Vertical bars on the map denote the positions and orientation of the Tn 3 - gusA insertion. The two lines with arrowheads beneath the restriction enzyme map indicate the direction and extent of transcription. <t>cDNA</t> synthesized by reverse transcriptase with primers RT1 and RT2 was analyzed by PCR. Thick bars indicate the six expected PCR products, which were verified by electrophoresis on 1.5% agarose gel. B . Photographs of swim assay plates showing the swimming motility of the BGR1 mutant fliA ::Tn 3 - gusA45 and complementation with pBGFA carrying the fliA gene at 28°C and 37°C. C . Expression levels of the flhF gene in the wild-type BGR1, the tofI mutant BGS2, and the qsmR mutant BGS9, and the flhC mutant BGF41 at 28°C, based on qRT-PCR analysis. Vertical lines indicate the standard deviations of three independent experiments.
    Flhf Cdna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flhf cdna/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flhf cdna - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

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    Genetic organization of flhBAFG and fliA genes, and flhF gene expression at 28°C. A . Genetic organization and transcriptional units of flhBAFG and fliA , with arrows showing the direction of transcription. Recognition sites for Eco RI and Stu I are indicated by E and S, respectively. Vertical bars on the map denote the positions and orientation of the Tn 3 - gusA insertion. The two lines with arrowheads beneath the restriction enzyme map indicate the direction and extent of transcription. cDNA synthesized by reverse transcriptase with primers RT1 and RT2 was analyzed by PCR. Thick bars indicate the six expected PCR products, which were verified by electrophoresis on 1.5% agarose gel. B . Photographs of swim assay plates showing the swimming motility of the BGR1 mutant fliA ::Tn 3 - gusA45 and complementation with pBGFA carrying the fliA gene at 28°C and 37°C. C . Expression levels of the flhF gene in the wild-type BGR1, the tofI mutant BGS2, and the qsmR mutant BGS9, and the flhC mutant BGF41 at 28°C, based on qRT-PCR analysis. Vertical lines indicate the standard deviations of three independent experiments.

    Journal: PLoS ONE

    Article Title: Quorum Sensing Controls Flagellar Morphogenesis in Burkholderia glumae

    doi: 10.1371/journal.pone.0084831

    Figure Lengend Snippet: Genetic organization of flhBAFG and fliA genes, and flhF gene expression at 28°C. A . Genetic organization and transcriptional units of flhBAFG and fliA , with arrows showing the direction of transcription. Recognition sites for Eco RI and Stu I are indicated by E and S, respectively. Vertical bars on the map denote the positions and orientation of the Tn 3 - gusA insertion. The two lines with arrowheads beneath the restriction enzyme map indicate the direction and extent of transcription. cDNA synthesized by reverse transcriptase with primers RT1 and RT2 was analyzed by PCR. Thick bars indicate the six expected PCR products, which were verified by electrophoresis on 1.5% agarose gel. B . Photographs of swim assay plates showing the swimming motility of the BGR1 mutant fliA ::Tn 3 - gusA45 and complementation with pBGFA carrying the fliA gene at 28°C and 37°C. C . Expression levels of the flhF gene in the wild-type BGR1, the tofI mutant BGS2, and the qsmR mutant BGS9, and the flhC mutant BGF41 at 28°C, based on qRT-PCR analysis. Vertical lines indicate the standard deviations of three independent experiments.

    Article Snippet: For qRT-PCR analysis, the primers of flhC-cDNA, flhF-cDNA, flhC-qRTF, flhC-qRTR, flhF-qRTF, and flhF-qRTR were used ( ). qRT-PCR analysis was performed according to the manufacturer's instructions except using SsoFast™ EvaGreen Supermix (Bio-Rad) with 25 ng of cDNA as template. qRT-PCR was performed using a thermal cycler (Model C1000™; Bio-Rad) under the following conditions: 98°C for 2 min, followed by 35 cycles at 98°C for 20 s, 65°C for 30 s, and 72°C for 30 s. The 16S rRNA gene was used for data normalization.

    Techniques: Expressing, Synthesized, Polymerase Chain Reaction, Electrophoresis, Agarose Gel Electrophoresis, Mutagenesis, Quantitative RT-PCR