wfa (Vector Laboratories)
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Name:
Fluorescein labeled Wisteria Floribunda Lectin WFA WFL
Description:
Wisteria floribunda lectin WFA WFL The binding specificity of Wisteria floribunda lectin WFL is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N acetylgalactosamine linked α or β to the 3 or 6 position of galactose This lectin has been used to fractionate lymphocyte populations and although not mitogenic elicits the production of lymphokines from murine splenocytes Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin This conjugate is supplied essentially free of unconjugated fluorochromes The excitation maximum is at 495 nm and the emission maximum is at 515 nm Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product All of these reagents are supplied as solutions perserved with sodium azide Inhibiting Eluting Sugar 200 mM N acetylgalactosamine
Catalog Number:
fl-1351
Price:
None
Category:
Proteins
Size:
2 mg
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Structured Review
Vector Laboratories
wfa
Wisteria floribunda lectin WFA WFL The binding specificity of Wisteria floribunda lectin WFL is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N acetylgalactosamine linked α or β to the 3 or 6 position of galactose This lectin has been used to fractionate lymphocyte populations and although not mitogenic elicits the production of lymphokines from murine splenocytes Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin This conjugate is supplied essentially free of unconjugated fluorochromes The excitation maximum is at 495 nm and the emission maximum is at 515 nm Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product All of these reagents are supplied as solutions perserved with sodium azide Inhibiting Eluting Sugar 200 mM N acetylgalactosamine
https://www.bioz.com/result/wfa/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Wisteria floribunda lectin WFA WFL The binding specificity of Wisteria floribunda lectin WFL is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N acetylgalactosamine linked α or β to the 3 or 6 position of galactose This lectin has been used to fractionate lymphocyte populations and although not mitogenic elicits the production of lymphokines from murine splenocytes Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin This conjugate is supplied essentially free of unconjugated fluorochromes The excitation maximum is at 495 nm and the emission maximum is at 515 nm Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product All of these reagents are supplied as solutions perserved with sodium azide Inhibiting Eluting Sugar 200 mM N acetylgalactosamine
https://www.bioz.com/result/wfa/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wfa - by Bioz Stars,
2021-03
93/100 stars
Images
1) Product Images from "Activity-Dependent Dysfunction in Visual and Olfactory Sensory Systems in Mouse Models of Down Syndrome"
Article Title: Activity-Dependent Dysfunction in Visual and Olfactory Sensory Systems in Mouse Models of Down Syndrome
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.1045-17.2017
Figure Legend Snippet: Histologic assessment of neuronal populations in the Ts65Dn visual cortex. A–O , Representative immunostains ( A–L ) and WFA staining ( M–O ) of nontrisomic littermate ( A , D , G , J , M ) and Ts65Dn ( B , E , H , K , N ) visual cortex. No significant differences were observed in the density of NeuN + ( A–C ), Gad67 + ( D–F ), parvalbumin + ( G–I ), somatostatin + ( J–L ), or WFA-stained neurons in Ts65Dn and nontrisomic visual cortex. C , F , I , L , O , Quantitation of cell counts in nontrisomic (black) and Ts65Dn (red) cortex. Scale bar, 500 μm. Error bars denote SD. n = 6 per group.
Techniques Used: Staining, Quantitation Assay
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Staining:Article Title: N-Acetylgalactosamine Positive Perineuronal Nets in the Saccade-Related-Part of the Cerebellar Fastigial Nucleus Do Not Maintain Saccade Gain Article Snippet: We mounted sections on charged slides, dehydrated them with increasing concentrations of ethanol, stained them with toluidine blue at pH 4.1 for ∼45 seconds, and then rehydrated them. .. We stained another set for intact perineuronal nets with Article Title: Activity-Dependent Dysfunction in Visual and Olfactory Sensory Systems in Mouse Models of Down Syndrome Article Snippet: .. Sections were stained by overnight incubation with guinea pig anti-Arc (catalog #156 005, Synaptic Systems; 1:1000), mouse anti-NeuN (MAB377, Millipore; 1:1000), mouse anti-parvalbumin (P3088, Sigma-Aldrich; 1:4000), rabbit anti-somatostatin (ab64053, Abcam; 1:1000), and mouse anti-Gad67 (MAB5406, Millipore; 1:1000) antibodies and/or Article Title: Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms Article Snippet: .. After digestion, biofilms were washed, and Pel was then stained with Article Title: A standardized and automated method of perineuronal net analysis using Wisteria floribunda agglutinin staining intensity Article Snippet: Coronal brain sections through the prefrontal cortex (PFC; +4.0 through +3.6 from bregma; Paxinos & Watson, 2007) and dorsal hippocampus (−2.0 through −4.0 from bregma; Paxinos & Watson, 2007) were cut at 30 µm using a freezing microtome. .. WFA staining was performed by washing free-floating sections three times for 5 min in 1×-PBS, the tissue was placed in 3% goat blocking serum (Vector Laboratories) for 1 h and was then incubated overnight at 4°C on a shaker table with Immunohistochemistry:Article Title: N-Acetylgalactosamine Positive Perineuronal Nets in the Saccade-Related-Part of the Cerebellar Fastigial Nucleus Do Not Maintain Saccade Gain Article Snippet: We mounted sections on charged slides, dehydrated them with increasing concentrations of ethanol, stained them with toluidine blue at pH 4.1 for ∼45 seconds, and then rehydrated them. .. We stained another set for intact perineuronal nets with Incubation:Article Title: Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex Article Snippet: .. They were then incubated for two hours in 594 goat-anti-rabbit IgG (1:1000, Invitrogen) for VGAT cells, 647 goat-anti-mouse IgG1 (1:1000, Invitrogen) for PV cells, and Article Title: Activity-Dependent Dysfunction in Visual and Olfactory Sensory Systems in Mouse Models of Down Syndrome Article Snippet: .. Sections were stained by overnight incubation with guinea pig anti-Arc (catalog #156 005, Synaptic Systems; 1:1000), mouse anti-NeuN (MAB377, Millipore; 1:1000), mouse anti-parvalbumin (P3088, Sigma-Aldrich; 1:4000), rabbit anti-somatostatin (ab64053, Abcam; 1:1000), and mouse anti-Gad67 (MAB5406, Millipore; 1:1000) antibodies and/or Article Title: Venlafaxine Stimulates an MMP-9-Dependent Increase in Excitatory/Inhibitory Balance in a Stress Model of Depression Article Snippet: Sections were washed 2 or 3 times with 1× PBS, then permeabilized with 1× PBS containing 0.1% Triton X-100, blocked with 10% normal goat serum, and incubated with anti-PV (1:500, Sigma Millipore, P3088) overnight at 4°C. .. Following subsequent washes and incubation with a fluorescent secondary antibody for PV immunostaining and Article Title: A standardized and automated method of perineuronal net analysis using Wisteria floribunda agglutinin staining intensity Article Snippet: Coronal brain sections through the prefrontal cortex (PFC; +4.0 through +3.6 from bregma; Paxinos & Watson, 2007) and dorsal hippocampus (−2.0 through −4.0 from bregma; Paxinos & Watson, 2007) were cut at 30 µm using a freezing microtome. .. WFA staining was performed by washing free-floating sections three times for 5 min in 1×-PBS, the tissue was placed in 3% goat blocking serum (Vector Laboratories) for 1 h and was then incubated overnight at 4°C on a shaker table with Labeling:Article Title: Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex Article Snippet: .. They were then incubated for two hours in 594 goat-anti-rabbit IgG (1:1000, Invitrogen) for VGAT cells, 647 goat-anti-mouse IgG1 (1:1000, Invitrogen) for PV cells, and Blocking Assay:Article Title: Activity-Dependent Dysfunction in Visual and Olfactory Sensory Systems in Mouse Models of Down Syndrome Article Snippet: .. Sections were stained by overnight incubation with guinea pig anti-Arc (catalog #156 005, Synaptic Systems; 1:1000), mouse anti-NeuN (MAB377, Millipore; 1:1000), mouse anti-parvalbumin (P3088, Sigma-Aldrich; 1:4000), rabbit anti-somatostatin (ab64053, Abcam; 1:1000), and mouse anti-Gad67 (MAB5406, Millipore; 1:1000) antibodies and/or Article Title: A standardized and automated method of perineuronal net analysis using Wisteria floribunda agglutinin staining intensity Article Snippet: Coronal brain sections through the prefrontal cortex (PFC; +4.0 through +3.6 from bregma; Paxinos & Watson, 2007) and dorsal hippocampus (−2.0 through −4.0 from bregma; Paxinos & Watson, 2007) were cut at 30 µm using a freezing microtome. .. WFA staining was performed by washing free-floating sections three times for 5 min in 1×-PBS, the tissue was placed in 3% goat blocking serum (Vector Laboratories) for 1 h and was then incubated overnight at 4°C on a shaker table with Immunostaining:Article Title: Venlafaxine Stimulates an MMP-9-Dependent Increase in Excitatory/Inhibitory Balance in a Stress Model of Depression Article Snippet: Sections were washed 2 or 3 times with 1× PBS, then permeabilized with 1× PBS containing 0.1% Triton X-100, blocked with 10% normal goat serum, and incubated with anti-PV (1:500, Sigma Millipore, P3088) overnight at 4°C. .. Following subsequent washes and incubation with a fluorescent secondary antibody for PV immunostaining and Binding Assay:Article Title: CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation Article Snippet: .. Expressing:Article Title: CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation Article Snippet: .. Laser-Scanning Microscopy:Article Title: Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms Article Snippet: .. After digestion, biofilms were washed, and Pel was then stained with |
