isolectin b4  (Vector Laboratories)

Journal: International Journal of Nanomedicine

Article Title: A Multifunctional Nanodrug Co-Delivering VEGF-siRNA and Dexamethasone for Synergistic Therapy in Ocular Neovascular Diseases

doi: 10.2147/IJN.S492363

Figure Lengend Snippet: Anti-angiogenic effects of DMAS in OIR model. ( A ) IB4-stained retinal whole-mount images of C57BL/6J mice at postnatal 17th day. White dashed lines outline non-perfused regions, and red dots indicate neovascularization. Scale bar: 1 mm. ( B and C ) The relative area of retinal neovascularization ( B ) and non-perfused zone ( C ) in OIR mice. ( D ) Effects of MPDA nanodrugs on VEGF expression in retinal homogenates of mice (n=4). ( E ) H&E staining results of endothelial cell nuclei breaking through the retinal inner limiting membrane. The white arrows indicate the locations of the endothelial cell nuclei. In the H&E images, a scale bar of 1 mm is used for the general view, while a scale bar of 100 μm is applied for the zoom-in view. ( F ) Quantification of the number of endothelial cell nuclei breaking through the retinal inner limiting membrane. The data are presented as mean ± SEM with * indicating P < 0.05, **indicating P < 0.01, ***indicating P < 0.001, ****indicating P < 0.0001, and ns indicating no significant difference.

Article Snippet: After three washes with PBS, the retinal samples and RPE/choroid were incubated with fluorescein-conjugated isolectin B4 (IB4, Cat# FL-1201, diluted at 1:100, Vector Laboratories, Burlingame, USA) at 4°C overnight.

Techniques: Staining, Expressing, Membrane

Journal: International Journal of Nanomedicine

Article Title: A Multifunctional Nanodrug Co-Delivering VEGF-siRNA and Dexamethasone for Synergistic Therapy in Ocular Neovascular Diseases

doi: 10.2147/IJN.S492363

Figure Lengend Snippet: Anti-angiogenic effects of DMAS in laser-induced CNV model. ( A ) Comparison of fundus images before and after laser-induced CNV modeling using scanning laser ophthalmoscopy (SLO), fundus autofluorescence (FAF), color fundus photography (CFP), IB4 staining, and fundus fluorescein angiography (FFA). White arrows indicate the locations of the optic disc. Scale bar: 100 μm. ( B ) Optical coherence tomography angiography (OCTA) images demonstrate the morphological characteristics of CNV, with white arrows indicating the optic disc and blue dashed lines representing the OCT scanning lines. Scale bar: 200 μm. ( C ) Observation of CNV lesion thickness in mice using OCT, with blue dashed lines representing OCT scanning lines, white arrows denoting the optic disc, and red dashed lines indicating CNV lesions. Scale bar: 200 μm. ( D ) Immunofluorescence staining with IB4 was used to assess the CNV area, with white arrows indicating the locations of the optic disc. The area outlined by the dashed white circular line indicates the CNV region. Scale bar: 200 μm. ( E and F ) Quantitative OCT results of laser-induced CNV lesion thickness ( E ) and area ( F ) in mice. The data are presented as mean ± SEM with *indicating P < 0.05, **indicating P < 0.01, and ns indicating no significant difference. Naïve refers to mice not subjected to the laser-induced CNV model.

Article Snippet: After three washes with PBS, the retinal samples and RPE/choroid were incubated with fluorescein-conjugated isolectin B4 (IB4, Cat# FL-1201, diluted at 1:100, Vector Laboratories, Burlingame, USA) at 4°C overnight.

Techniques: Comparison, Staining, Tomography, Immunofluorescence