gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
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    lectin fl 1201  (Vector Laboratories)


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    lectin fl 1201  (Vector Laboratories)


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    Vector Laboratories lectin fl 1201
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    gsl i isolectin b4  (Vector Laboratories)


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    fluorochromes with fitc  (Vector Laboratories)


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    The detail information of primers, antibody and siRNA.
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    Images

    1) Product Images from "Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice"

    Article Title: Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice

    Journal: Theranostics

    doi: 10.7150/thno.19500

    The detail information of primers, antibody and siRNA.
    Figure Legend Snippet: The detail information of primers, antibody and siRNA.

    Techniques Used: Western Blot, Immunohistochemical staining, Staining, Plasmid Preparation

    MT1 and MT2 had different expression patterns in the DRG in control C57BL/6J mice. (A-E) Immunofluorescence staining of MT1 and GFAP in the DRG in control C57BL/6J mice: Panel D is the merged image of panel A, B, and C. Panel E shows a magnified section of the panel D (bar=30 μm in A-D; bar=9 μm in E); (F-J) Immunofluorescence staining of MT2 and NeuN in the DRG in control C57BL/6J mice: Panel I is the merged image of panel F, G and H. Panel J is a magnified section of the panel I (bar=30 μm in F-I; bar=9 μm in J); (K-N) DAB staining of MT1 and MT2 in the DRG in control C57BL/6J mice: Panels L and N show magnified sections of panel K and M, respectively. The arrows in L indicate the MT1-immunoreactive satellite cells. The arrows in N represent the MT2-immunoreactive neurons. (bar=80 μm in K and M; bar=18 μm in L and N.); (O) Bar chart representing the soma diameters of the high MT2-expressing neurons ( *** P <0.001; n=8 for each group); (P-Y) Double-label staining of MT2 and CGRP/IB4. Panels T and Y are magnified sections of merged panels S and X, respectively (bar=30 μm in P-S and U-X; bar=9 μm in T and Y).
    Figure Legend Snippet: MT1 and MT2 had different expression patterns in the DRG in control C57BL/6J mice. (A-E) Immunofluorescence staining of MT1 and GFAP in the DRG in control C57BL/6J mice: Panel D is the merged image of panel A, B, and C. Panel E shows a magnified section of the panel D (bar=30 μm in A-D; bar=9 μm in E); (F-J) Immunofluorescence staining of MT2 and NeuN in the DRG in control C57BL/6J mice: Panel I is the merged image of panel F, G and H. Panel J is a magnified section of the panel I (bar=30 μm in F-I; bar=9 μm in J); (K-N) DAB staining of MT1 and MT2 in the DRG in control C57BL/6J mice: Panels L and N show magnified sections of panel K and M, respectively. The arrows in L indicate the MT1-immunoreactive satellite cells. The arrows in N represent the MT2-immunoreactive neurons. (bar=80 μm in K and M; bar=18 μm in L and N.); (O) Bar chart representing the soma diameters of the high MT2-expressing neurons ( *** P <0.001; n=8 for each group); (P-Y) Double-label staining of MT2 and CGRP/IB4. Panels T and Y are magnified sections of merged panels S and X, respectively (bar=30 μm in P-S and U-X; bar=9 μm in T and Y).

    Techniques Used: Expressing, Immunofluorescence, Staining

    fluorescein 488 gs1β 4  (Vector Laboratories)


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    Vector Laboratories fluorescein 488 gs1β 4
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    isolectin  (Vector Laboratories)


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    fluorescein labeled isolectin b4  (Vector Laboratories)


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    isolectin b4  (Vector Laboratories)


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    fluorescein griffonia simplicifolia lectin b4  (Vector Laboratories)


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    biotinylated isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotinylated isolectin b 4
    Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and <t>IB</t> <t>4</t> positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).
    Biotinylated Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Intrinsic Up-Regulation of 2-AG Favors an Area Specific Neuronal Survival in Different In Vitro Models of Neuronal Damage"

    Article Title: Intrinsic Up-Regulation of 2-AG Favors an Area Specific Neuronal Survival in Different In Vitro Models of Neuronal Damage

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0051208

    Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and IB 4 positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).
    Figure Legend Snippet: Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and IB 4 positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).

    Techniques Used: Staining, Labeling

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    Vector Laboratories gsl i isolectin b4
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    Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and <t>IB</t> <t>4</t> positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).
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    Image Search Results


    The detail information of primers, antibody and siRNA.

    Journal: Theranostics

    Article Title: Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice

    doi: 10.7150/thno.19500

    Figure Lengend Snippet: The detail information of primers, antibody and siRNA.

    Article Snippet: rabbit anti-MT1: ab203038, 1:100, Abcam; rabbit anti-MT2: ab203346, 1:100, Abcam; chicken anti-NeuN: ab134014, 1:1000, Abcam; chicken anti-GFAP: ab50738, 1:100, rabbit anti-CGRP: AB5920, 1:1000, Millipore; anti-rabbit-conjugated AlexaFluor 594: ab150116, 1:1000, Abcam; anti-chicken-conjugated AlexaFluor 488: A-11039, 1:1000, Thermo Fisher Scientific, Shanghai, China. The IB4 were detected with fluorochromes with FITC (FITC-IB4: FL-1201, 1:200, Vector). , .

    Techniques: Western Blot, Immunohistochemical staining, Staining, Plasmid Preparation

    MT1 and MT2 had different expression patterns in the DRG in control C57BL/6J mice. (A-E) Immunofluorescence staining of MT1 and GFAP in the DRG in control C57BL/6J mice: Panel D is the merged image of panel A, B, and C. Panel E shows a magnified section of the panel D (bar=30 μm in A-D; bar=9 μm in E); (F-J) Immunofluorescence staining of MT2 and NeuN in the DRG in control C57BL/6J mice: Panel I is the merged image of panel F, G and H. Panel J is a magnified section of the panel I (bar=30 μm in F-I; bar=9 μm in J); (K-N) DAB staining of MT1 and MT2 in the DRG in control C57BL/6J mice: Panels L and N show magnified sections of panel K and M, respectively. The arrows in L indicate the MT1-immunoreactive satellite cells. The arrows in N represent the MT2-immunoreactive neurons. (bar=80 μm in K and M; bar=18 μm in L and N.); (O) Bar chart representing the soma diameters of the high MT2-expressing neurons ( *** P <0.001; n=8 for each group); (P-Y) Double-label staining of MT2 and CGRP/IB4. Panels T and Y are magnified sections of merged panels S and X, respectively (bar=30 μm in P-S and U-X; bar=9 μm in T and Y).

    Journal: Theranostics

    Article Title: Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice

    doi: 10.7150/thno.19500

    Figure Lengend Snippet: MT1 and MT2 had different expression patterns in the DRG in control C57BL/6J mice. (A-E) Immunofluorescence staining of MT1 and GFAP in the DRG in control C57BL/6J mice: Panel D is the merged image of panel A, B, and C. Panel E shows a magnified section of the panel D (bar=30 μm in A-D; bar=9 μm in E); (F-J) Immunofluorescence staining of MT2 and NeuN in the DRG in control C57BL/6J mice: Panel I is the merged image of panel F, G and H. Panel J is a magnified section of the panel I (bar=30 μm in F-I; bar=9 μm in J); (K-N) DAB staining of MT1 and MT2 in the DRG in control C57BL/6J mice: Panels L and N show magnified sections of panel K and M, respectively. The arrows in L indicate the MT1-immunoreactive satellite cells. The arrows in N represent the MT2-immunoreactive neurons. (bar=80 μm in K and M; bar=18 μm in L and N.); (O) Bar chart representing the soma diameters of the high MT2-expressing neurons ( *** P <0.001; n=8 for each group); (P-Y) Double-label staining of MT2 and CGRP/IB4. Panels T and Y are magnified sections of merged panels S and X, respectively (bar=30 μm in P-S and U-X; bar=9 μm in T and Y).

    Article Snippet: rabbit anti-MT1: ab203038, 1:100, Abcam; rabbit anti-MT2: ab203346, 1:100, Abcam; chicken anti-NeuN: ab134014, 1:1000, Abcam; chicken anti-GFAP: ab50738, 1:100, rabbit anti-CGRP: AB5920, 1:1000, Millipore; anti-rabbit-conjugated AlexaFluor 594: ab150116, 1:1000, Abcam; anti-chicken-conjugated AlexaFluor 488: A-11039, 1:1000, Thermo Fisher Scientific, Shanghai, China. The IB4 were detected with fluorochromes with FITC (FITC-IB4: FL-1201, 1:200, Vector). , .

    Techniques: Expressing, Immunofluorescence, Staining

    Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and IB 4 positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).

    Journal: PLoS ONE

    Article Title: Intrinsic Up-Regulation of 2-AG Favors an Area Specific Neuronal Survival in Different In Vitro Models of Neuronal Damage

    doi: 10.1371/journal.pone.0051208

    Figure Lengend Snippet: Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and IB 4 positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).

    Article Snippet: After blocking unspecific bindings by normal goat serum (1∶20 in PB saline (PBS)/Triton) for 30 min primary antibodies were added for 16 h in PBS/Triton containing 0.5% (w/v) bovine serum albumin: DAGL (diluted 1∶200, Frontier Science, cat.-no DGLa-Rb-Af380), MAGL (diluted 1∶200, Cayman Chemicals, cat no. 100035, ), Vglut1 (diluted 1∶1000, synaptic systems, cat no. 135311), NeuN (diluted 1∶200; cat.-No MAB377, Millipore), GFAP (diluted 1∶200; cat. No 556330 BD Pharmingen) and biotinylated isolectin B 4 (IB 4 ) (diluted 1∶50; cat.-No FL-1201 Vector Laboratories).

    Techniques: Staining, Labeling