fluorescein conjugated dolichos biflorus agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated dolichos biflorus agglutinin
    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. <t>biflorus</t> lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
    Fluorescein Conjugated Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein conjugated dolichos biflorus agglutinin/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    Images

    1) Product Images from "The apicoplast is important for the viability and persistence of Toxoplasma gondii bradyzoites"

    Article Title: The apicoplast is important for the viability and persistence of Toxoplasma gondii bradyzoites

    Journal: bioRxiv

    doi: 10.1101/2023.01.13.523885

    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. biflorus lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
    Figure Legend Snippet: A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. biflorus lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.

    Techniques Used: Homologous Recombination, Western Blot, In Vitro, Expressing, Imaging, Generated

    fluorescein conjugated dolichos biflorus agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated dolichos biflorus agglutinin
    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. <t>biflorus</t> lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
    Fluorescein Conjugated Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein conjugated dolichos biflorus agglutinin/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    94/100 stars

    Images

    1) Product Images from "The apicoplast is important for the viability and persistence of Toxoplasma gondii bradyzoites"

    Article Title: The apicoplast is important for the viability and persistence of Toxoplasma gondii bradyzoites

    Journal: bioRxiv

    doi: 10.1101/2023.01.13.523885

    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. biflorus lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
    Figure Legend Snippet: A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. biflorus lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.

    Techniques Used: Homologous Recombination, Western Blot, In Vitro, Expressing, Imaging, Generated

    fluorescein labeled dolichos biflorus agglutinin (dba)  (Vector Laboratories)


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    Vector Laboratories fluorescein labeled dolichos biflorus agglutinin (dba)
    Fluorescein Labeled Dolichos Biflorus Agglutinin (Dba), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein labeled dolichos biflorus agglutinin (dba)/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    94/100 stars

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    fluorescein dolichos biflorus d biflorus agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein dolichos biflorus d biflorus agglutinin
    Fluorescein Dolichos Biflorus D Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein dolichos biflorus d biflorus agglutinin/product/Vector Laboratories
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    fluorescein dolichos biflorus d biflorus agglutinin - by Bioz Stars, 2024-05
    94/100 stars

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    dolichos biflorus agglutinin dba  (Vector Laboratories)


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    Vector Laboratories dolichos biflorus agglutinin dba
    Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dolichos biflorus agglutinin dba/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    fluorescein labeled dolichos biflorus agglutinin dba  (Vector Laboratories)


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    Vector Laboratories fluorescein labeled dolichos biflorus agglutinin dba
    Antibodies
    Fluorescein Labeled Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein labeled dolichos biflorus agglutinin dba/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    fluorescein labeled dolichos biflorus agglutinin dba - by Bioz Stars, 2024-05
    94/100 stars

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    1) Product Images from "Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia"

    Article Title: Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    doi: 10.1016/j.jcmgh.2022.06.001

    Antibodies
    Figure Legend Snippet: Antibodies

    Techniques Used: Immunofluorescence, Western Blot, Plasmid Preparation, Labeling

    dolichos biflorus agglutinin fluorescein  (Vector Laboratories)


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    Vector Laboratories dolichos biflorus agglutinin fluorescein
    TgH1-like is present only in bradyzoites undergoing division. (A) IFA of Pru TgH1-like HA was performed after 3 to 4 days under bradyzoite conversion. <t>Fluorescein-labeled</t> <t>Dolichos</t> <t>biflorus</t> agglutinin (DBA) was used to visualize cyst walls. (LDH2-GFP reporter is also present in bradyzoites in this channel.) (B) IMC3 antibody was used to observe parasites under division. Scale bars = 5 μm. (C) Western blotting showed a faint band of 20 kDa in Pru TgH1-like HA . T. gondii β-tubulin was used as a loading control.
    Dolichos Biflorus Agglutinin Fluorescein, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dolichos biflorus agglutinin fluorescein/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    dolichos biflorus agglutinin fluorescein - by Bioz Stars, 2024-05
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    1) Product Images from "Previously Unidentified Histone H1-Like Protein Is Involved in Cell Division and Ribosome Biosynthesis in Toxoplasma gondii"

    Article Title: Previously Unidentified Histone H1-Like Protein Is Involved in Cell Division and Ribosome Biosynthesis in Toxoplasma gondii

    Journal: mSphere

    doi: 10.1128/msphere.00403-22

    TgH1-like is present only in bradyzoites undergoing division. (A) IFA of Pru TgH1-like HA was performed after 3 to 4 days under bradyzoite conversion. Fluorescein-labeled Dolichos biflorus agglutinin (DBA) was used to visualize cyst walls. (LDH2-GFP reporter is also present in bradyzoites in this channel.) (B) IMC3 antibody was used to observe parasites under division. Scale bars = 5 μm. (C) Western blotting showed a faint band of 20 kDa in Pru TgH1-like HA . T. gondii β-tubulin was used as a loading control.
    Figure Legend Snippet: TgH1-like is present only in bradyzoites undergoing division. (A) IFA of Pru TgH1-like HA was performed after 3 to 4 days under bradyzoite conversion. Fluorescein-labeled Dolichos biflorus agglutinin (DBA) was used to visualize cyst walls. (LDH2-GFP reporter is also present in bradyzoites in this channel.) (B) IMC3 antibody was used to observe parasites under division. Scale bars = 5 μm. (C) Western blotting showed a faint band of 20 kDa in Pru TgH1-like HA . T. gondii β-tubulin was used as a loading control.

    Techniques Used: Labeling, Western Blot

    fluorescein conjugated dolichos biflorus agglutinin dba  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated dolichos biflorus agglutinin dba
    Goblet cell metaplasia in the alveolar epithelium . A-B: IHC with Rhodamine-conjugated Dolichos <t>Biflorus</t> Agglutinin <t>(DBA)</t> and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.
    Fluorescein Conjugated Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    1) Product Images from "FGF10 maintains distal lung bud epithelium and excessive signaling leads to progenitor state arrest, distalization, and goblet cell metaplasia"

    Article Title: FGF10 maintains distal lung bud epithelium and excessive signaling leads to progenitor state arrest, distalization, and goblet cell metaplasia

    Journal: BMC Developmental Biology

    doi: 10.1186/1471-213X-8-2

    Goblet cell metaplasia in the alveolar epithelium . A-B: IHC with Rhodamine-conjugated Dolichos Biflorus Agglutinin (DBA) and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.
    Figure Legend Snippet: Goblet cell metaplasia in the alveolar epithelium . A-B: IHC with Rhodamine-conjugated Dolichos Biflorus Agglutinin (DBA) and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.

    Techniques Used: Transgenic Assay

    fluorescein  (Vector Laboratories)


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    Vector Laboratories fluorescein
    Fluorescein, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fluorescein conjugated lectin dolichos biflorus agglutinin dba  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated lectin dolichos biflorus agglutinin dba
    Identification of gonocytes with <t>DBA</t> staining, following the masking of autofluorescence by Sudan Black B in situ and in vitro . Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with FITC-labeled <t>lectin</t> DBA and DAPI, followed by Sudan Black B staining and imaging with a confocal laser scanning microscope with brightfield overlay. Scale bars, 100 μ m.
    Fluorescein Conjugated Lectin Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein conjugated lectin dolichos biflorus agglutinin dba/product/Vector Laboratories
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    1) Product Images from "Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells"

    Article Title: Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells

    Journal: Anatomy Research International

    doi: 10.1155/2012/820120

    Identification of gonocytes with DBA staining, following the masking of autofluorescence by Sudan Black B in situ and in vitro . Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with FITC-labeled lectin DBA and DAPI, followed by Sudan Black B staining and imaging with a confocal laser scanning microscope with brightfield overlay. Scale bars, 100 μ m.
    Figure Legend Snippet: Identification of gonocytes with DBA staining, following the masking of autofluorescence by Sudan Black B in situ and in vitro . Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with FITC-labeled lectin DBA and DAPI, followed by Sudan Black B staining and imaging with a confocal laser scanning microscope with brightfield overlay. Scale bars, 100 μ m.

    Techniques Used: Staining, In Situ, In Vitro, Labeling, Imaging, Laser-Scanning Microscopy

    fluorescein dolichos biflorus agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein dolichos biflorus agglutinin
    Fluorescein Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein dolichos biflorus agglutinin/product/Vector Laboratories
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    Vector Laboratories fluorescein conjugated dolichos biflorus agglutinin
    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. <t>biflorus</t> lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
    Fluorescein Conjugated Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories fluorescein labeled dolichos biflorus agglutinin (dba)
    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. <t>biflorus</t> lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
    Fluorescein Labeled Dolichos Biflorus Agglutinin (Dba), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein labeled dolichos biflorus agglutinin (dba)/product/Vector Laboratories
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    94
    Vector Laboratories fluorescein dolichos biflorus d biflorus agglutinin
    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. <t>biflorus</t> lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
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    Vector Laboratories dolichos biflorus agglutinin dba
    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. <t>biflorus</t> lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.
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    Antibodies
    Fluorescein Labeled Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TgH1-like is present only in bradyzoites undergoing division. (A) IFA of Pru TgH1-like HA was performed after 3 to 4 days under bradyzoite conversion. <t>Fluorescein-labeled</t> <t>Dolichos</t> <t>biflorus</t> agglutinin (DBA) was used to visualize cyst walls. (LDH2-GFP reporter is also present in bradyzoites in this channel.) (B) IMC3 antibody was used to observe parasites under division. Scale bars = 5 μm. (C) Western blotting showed a faint band of 20 kDa in Pru TgH1-like HA . T. gondii β-tubulin was used as a loading control.
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    Goblet cell metaplasia in the alveolar epithelium . A-B: IHC with Rhodamine-conjugated Dolichos <t>Biflorus</t> Agglutinin <t>(DBA)</t> and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.
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    Goblet cell metaplasia in the alveolar epithelium . A-B: IHC with Rhodamine-conjugated Dolichos <t>Biflorus</t> Agglutinin <t>(DBA)</t> and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.
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    Identification of gonocytes with <t>DBA</t> staining, following the masking of autofluorescence by Sudan Black B in situ and in vitro . Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with FITC-labeled <t>lectin</t> DBA and DAPI, followed by Sudan Black B staining and imaging with a confocal laser scanning microscope with brightfield overlay. Scale bars, 100 μ m.
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    Vector Laboratories fluorescein dolichos biflorus agglutinin
    Identification of gonocytes with <t>DBA</t> staining, following the masking of autofluorescence by Sudan Black B in situ and in vitro . Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with FITC-labeled <t>lectin</t> DBA and DAPI, followed by Sudan Black B staining and imaging with a confocal laser scanning microscope with brightfield overlay. Scale bars, 100 μ m.
    Fluorescein Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. biflorus lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.

    Journal: bioRxiv

    Article Title: The apicoplast is important for the viability and persistence of Toxoplasma gondii bradyzoites

    doi: 10.1101/2023.01.13.523885

    Figure Lengend Snippet: A) Schematic representation of APT1 function at the apicoplast and its implication upstream of two important metabolic pathways. B) Scheme describing the strategy to edit the APT1 locus by homologous recombination, to express a copy of the APT1 gene under the dependence of a tachyzoite-specific SAG1 promoter and allowing fusion of APT1 with a C-terminal GFP. C) Immunoblot analysis of protein extracts from tachyzoites (Tz) and 12 days in vitro -differentiated (alkaline stress) bradyzoites (Bz) with an anti-GFP antibody to detect the expression of APT1-GFP (arrowhead) under the dependence of the SAG1 promoter. Actin was used as a loading control. D) Microscopic imaging of pSAG1-APT1 parasites submitted to alkaline pH stress for 7 days. Cyst walls were labelled with D. biflorus lectin (DBL) or outlined with dashed lines. A more intense DBL labelling outlines a more mature cyst (arrowhead). DNA was labelled with DAPI. Scale bar=10 µm. E) The GFP-APT1 signal in vacuoles containing tachyzoites, or cysts generated by alkaline pH-induced differentiation, was quantified and shows progressive signal loss upon stage conversion. Data are mean from n =3 independent experiments +SD. *** p ≤ 0.001, **** p ≤ 0.0001, by one-way ANOVA followed by Tukey post-hoc test.

    Article Snippet: For cyst enumeration, 5% of the homogenate was stained with fluorescein-conjugated Dolichos biflorus agglutinin (Vector Laboratories).

    Techniques: Homologous Recombination, Western Blot, In Vitro, Expressing, Imaging, Generated

    Antibodies

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia

    doi: 10.1016/j.jcmgh.2022.06.001

    Figure Lengend Snippet: Antibodies

    Article Snippet: Fluorescein labeled Dolichos Biflorus Agglutinin (DBA) , Vector Labs , FL-1031 , Immunofluorescence , 1:500.

    Techniques: Immunofluorescence, Western Blot, Plasmid Preparation, Labeling

    TgH1-like is present only in bradyzoites undergoing division. (A) IFA of Pru TgH1-like HA was performed after 3 to 4 days under bradyzoite conversion. Fluorescein-labeled Dolichos biflorus agglutinin (DBA) was used to visualize cyst walls. (LDH2-GFP reporter is also present in bradyzoites in this channel.) (B) IMC3 antibody was used to observe parasites under division. Scale bars = 5 μm. (C) Western blotting showed a faint band of 20 kDa in Pru TgH1-like HA . T. gondii β-tubulin was used as a loading control.

    Journal: mSphere

    Article Title: Previously Unidentified Histone H1-Like Protein Is Involved in Cell Division and Ribosome Biosynthesis in Toxoplasma gondii

    doi: 10.1128/msphere.00403-22

    Figure Lengend Snippet: TgH1-like is present only in bradyzoites undergoing division. (A) IFA of Pru TgH1-like HA was performed after 3 to 4 days under bradyzoite conversion. Fluorescein-labeled Dolichos biflorus agglutinin (DBA) was used to visualize cyst walls. (LDH2-GFP reporter is also present in bradyzoites in this channel.) (B) IMC3 antibody was used to observe parasites under division. Scale bars = 5 μm. (C) Western blotting showed a faint band of 20 kDa in Pru TgH1-like HA . T. gondii β-tubulin was used as a loading control.

    Article Snippet: For the DNA dye, Dolichos biflorus agglutinin fluorescein-labeled (2 mg at 1:250) (NC1112173 Vector Laboratories) DAPI (4′,6-diamidino-2-phenylindole [5 μg/mL]) was used.

    Techniques: Labeling, Western Blot

    Goblet cell metaplasia in the alveolar epithelium . A-B: IHC with Rhodamine-conjugated Dolichos Biflorus Agglutinin (DBA) and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.

    Journal: BMC Developmental Biology

    Article Title: FGF10 maintains distal lung bud epithelium and excessive signaling leads to progenitor state arrest, distalization, and goblet cell metaplasia

    doi: 10.1186/1471-213X-8-2

    Figure Lengend Snippet: Goblet cell metaplasia in the alveolar epithelium . A-B: IHC with Rhodamine-conjugated Dolichos Biflorus Agglutinin (DBA) and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.

    Article Snippet: Mature goblet cells were detected by an overnight staining using rhodamine or fluorescein conjugated Dolichos Biflorus Agglutinin (DBA) (Vector Laboratories).

    Techniques: Transgenic Assay

    Identification of gonocytes with DBA staining, following the masking of autofluorescence by Sudan Black B in situ and in vitro . Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with FITC-labeled lectin DBA and DAPI, followed by Sudan Black B staining and imaging with a confocal laser scanning microscope with brightfield overlay. Scale bars, 100 μ m.

    Journal: Anatomy Research International

    Article Title: Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells

    doi: 10.1155/2012/820120

    Figure Lengend Snippet: Identification of gonocytes with DBA staining, following the masking of autofluorescence by Sudan Black B in situ and in vitro . Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with FITC-labeled lectin DBA and DAPI, followed by Sudan Black B staining and imaging with a confocal laser scanning microscope with brightfield overlay. Scale bars, 100 μ m.

    Article Snippet: After deparaffinisation and rehydration, the processed testis tissue sections were rinsed with DPBS and incubated with 5% w/v bovine serum albumin (BSA, cat. no. A7638, Sigma-Aldrich) in DPBS at 37°C for 30 min in humidified atmosphere and stained overnight with a fluorescein-conjugated lectin Dolichos biflorus agglutinin (DBA) [ ] (1 : 100, cat. no. FL-1031, Vector Labs, Burlington, ON, Canada) in humidified atmosphere.

    Techniques: Staining, In Situ, In Vitro, Labeling, Imaging, Laser-Scanning Microscopy