wheat germ agglutinin  (Vector Laboratories)


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    Vector Laboratories wheat germ agglutinin
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    wheat germ agglutinin  (Vector Laboratories)


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    Vector Laboratories wheat germ agglutinin
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    fitc wga  (Vector Laboratories)


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    fluorescein conjugated wheat germ agglutinin wga  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated wheat germ agglutinin wga
    Cfh localization within rod and cone photoreceptors. A sagittal section from a male BALB/c mouse eye immunolabeled for rhodopsin (green), Cfh (red), and a 4',6-diamidino-2-phenylindole (DAPI) nuclear stain (blue) showed only a localized band of Cfh in the central region of the OS (white arrow), but not throughout the OS. In addition, occasional Cfh labeling of cells believed to be cone cell IS was observed (white arrowheads; A ). A sagittal section from a male BALB/c mouse eye immunolabeled for blue and red/green opsins (white), Cfh (red), and a DAPI nuclear stain (blue) showed a similar band of Cfh in the central region of the OS (green arrow; B ). Cone cells labeled with the opsin antibodies (green arrowheads) exhibit a polarized distribution of blue and red/green opsins (white) and Cfh (red). A coronal section from a male C57BL/6 eye double labeled <t>with</t> <t>fluorescein</t> conjugated <t>WGA</t> (green), which labels the matrix domains surrounding rod photoreceptors, and Cfh (red) showing Cfh within rod photoreceptor OS ( C ). The delineated area of interest is magnified and shown on the right. Cfh is clearly localized within the rods (arrows; D ). A coronal section from a male C57BL/6 eye labeled with fluorescein conjugated PNA (green), which binds cone matrix domains, and Cfh (red) showing Cfh is clearly localized within the cone cells (arrows; E ). The delineated area of interest is magnified and shown on the right ( F ). IS=photoreceptor inner segments; OS=outer segments; RPE=retinal pigment epithelium; WGA=wheat germ agglutinin; PNA=peanut agglutinin. Scale bar=20 μm.
    Fluorescein Conjugated Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Localization of complement factor H gene expression and protein distribution in the mouse outer retina"

    Article Title: Localization of complement factor H gene expression and protein distribution in the mouse outer retina

    Journal: Molecular Vision

    doi:

    Cfh localization within rod and cone photoreceptors. A sagittal section from a male BALB/c mouse eye immunolabeled for rhodopsin (green), Cfh (red), and a 4',6-diamidino-2-phenylindole (DAPI) nuclear stain (blue) showed only a localized band of Cfh in the central region of the OS (white arrow), but not throughout the OS. In addition, occasional Cfh labeling of cells believed to be cone cell IS was observed (white arrowheads; A ). A sagittal section from a male BALB/c mouse eye immunolabeled for blue and red/green opsins (white), Cfh (red), and a DAPI nuclear stain (blue) showed a similar band of Cfh in the central region of the OS (green arrow; B ). Cone cells labeled with the opsin antibodies (green arrowheads) exhibit a polarized distribution of blue and red/green opsins (white) and Cfh (red). A coronal section from a male C57BL/6 eye double labeled with fluorescein conjugated WGA (green), which labels the matrix domains surrounding rod photoreceptors, and Cfh (red) showing Cfh within rod photoreceptor OS ( C ). The delineated area of interest is magnified and shown on the right. Cfh is clearly localized within the rods (arrows; D ). A coronal section from a male C57BL/6 eye labeled with fluorescein conjugated PNA (green), which binds cone matrix domains, and Cfh (red) showing Cfh is clearly localized within the cone cells (arrows; E ). The delineated area of interest is magnified and shown on the right ( F ). IS=photoreceptor inner segments; OS=outer segments; RPE=retinal pigment epithelium; WGA=wheat germ agglutinin; PNA=peanut agglutinin. Scale bar=20 μm.
    Figure Legend Snippet: Cfh localization within rod and cone photoreceptors. A sagittal section from a male BALB/c mouse eye immunolabeled for rhodopsin (green), Cfh (red), and a 4',6-diamidino-2-phenylindole (DAPI) nuclear stain (blue) showed only a localized band of Cfh in the central region of the OS (white arrow), but not throughout the OS. In addition, occasional Cfh labeling of cells believed to be cone cell IS was observed (white arrowheads; A ). A sagittal section from a male BALB/c mouse eye immunolabeled for blue and red/green opsins (white), Cfh (red), and a DAPI nuclear stain (blue) showed a similar band of Cfh in the central region of the OS (green arrow; B ). Cone cells labeled with the opsin antibodies (green arrowheads) exhibit a polarized distribution of blue and red/green opsins (white) and Cfh (red). A coronal section from a male C57BL/6 eye double labeled with fluorescein conjugated WGA (green), which labels the matrix domains surrounding rod photoreceptors, and Cfh (red) showing Cfh within rod photoreceptor OS ( C ). The delineated area of interest is magnified and shown on the right. Cfh is clearly localized within the rods (arrows; D ). A coronal section from a male C57BL/6 eye labeled with fluorescein conjugated PNA (green), which binds cone matrix domains, and Cfh (red) showing Cfh is clearly localized within the cone cells (arrows; E ). The delineated area of interest is magnified and shown on the right ( F ). IS=photoreceptor inner segments; OS=outer segments; RPE=retinal pigment epithelium; WGA=wheat germ agglutinin; PNA=peanut agglutinin. Scale bar=20 μm.

    Techniques Used: Immunolabeling, Staining, Labeling

    peanut agglutinin  (Vector Laboratories)


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    Vector Laboratories peanut agglutinin
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    fluorescein wheat germ agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein wheat germ agglutinin
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    fluorescein  (Vector Laboratories)


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    Vector Laboratories fluorescein
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    fluorescein conjugated wheat germ agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated wheat germ agglutinin
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    fluorescein wheat germ agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein wheat germ agglutinin
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    fitc conjugated wheat germ agglutinin  (Vector Laboratories)


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    Vector Laboratories fitc conjugated wheat germ agglutinin
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    fluorescein wheat germ agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein wheat germ agglutinin
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    Cfh localization within rod and cone photoreceptors. A sagittal section from a male BALB/c mouse eye immunolabeled for rhodopsin (green), Cfh (red), and a 4',6-diamidino-2-phenylindole (DAPI) nuclear stain (blue) showed only a localized band of Cfh in the central region of the OS (white arrow), but not throughout the OS. In addition, occasional Cfh labeling of cells believed to be cone cell IS was observed (white arrowheads; A ). A sagittal section from a male BALB/c mouse eye immunolabeled for blue and red/green opsins (white), Cfh (red), and a DAPI nuclear stain (blue) showed a similar band of Cfh in the central region of the OS (green arrow; B ). Cone cells labeled with the opsin antibodies (green arrowheads) exhibit a polarized distribution of blue and red/green opsins (white) and Cfh (red). A coronal section from a male C57BL/6 eye double labeled <t>with</t> <t>fluorescein</t> conjugated <t>WGA</t> (green), which labels the matrix domains surrounding rod photoreceptors, and Cfh (red) showing Cfh within rod photoreceptor OS ( C ). The delineated area of interest is magnified and shown on the right. Cfh is clearly localized within the rods (arrows; D ). A coronal section from a male C57BL/6 eye labeled with fluorescein conjugated PNA (green), which binds cone matrix domains, and Cfh (red) showing Cfh is clearly localized within the cone cells (arrows; E ). The delineated area of interest is magnified and shown on the right ( F ). IS=photoreceptor inner segments; OS=outer segments; RPE=retinal pigment epithelium; WGA=wheat germ agglutinin; PNA=peanut agglutinin. Scale bar=20 μm.
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    Cfh localization within rod and cone photoreceptors. A sagittal section from a male BALB/c mouse eye immunolabeled for rhodopsin (green), Cfh (red), and a 4',6-diamidino-2-phenylindole (DAPI) nuclear stain (blue) showed only a localized band of Cfh in the central region of the OS (white arrow), but not throughout the OS. In addition, occasional Cfh labeling of cells believed to be cone cell IS was observed (white arrowheads; A ). A sagittal section from a male BALB/c mouse eye immunolabeled for blue and red/green opsins (white), Cfh (red), and a DAPI nuclear stain (blue) showed a similar band of Cfh in the central region of the OS (green arrow; B ). Cone cells labeled with the opsin antibodies (green arrowheads) exhibit a polarized distribution of blue and red/green opsins (white) and Cfh (red). A coronal section from a male C57BL/6 eye double labeled <t>with</t> <t>fluorescein</t> conjugated <t>WGA</t> (green), which labels the matrix domains surrounding rod photoreceptors, and Cfh (red) showing Cfh within rod photoreceptor OS ( C ). The delineated area of interest is magnified and shown on the right. Cfh is clearly localized within the rods (arrows; D ). A coronal section from a male C57BL/6 eye labeled with fluorescein conjugated PNA (green), which binds cone matrix domains, and Cfh (red) showing Cfh is clearly localized within the cone cells (arrows; E ). The delineated area of interest is magnified and shown on the right ( F ). IS=photoreceptor inner segments; OS=outer segments; RPE=retinal pigment epithelium; WGA=wheat germ agglutinin; PNA=peanut agglutinin. Scale bar=20 μm.
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    Cfh localization within rod and cone photoreceptors. A sagittal section from a male BALB/c mouse eye immunolabeled for rhodopsin (green), Cfh (red), and a 4',6-diamidino-2-phenylindole (DAPI) nuclear stain (blue) showed only a localized band of Cfh in the central region of the OS (white arrow), but not throughout the OS. In addition, occasional Cfh labeling of cells believed to be cone cell IS was observed (white arrowheads; A ). A sagittal section from a male BALB/c mouse eye immunolabeled for blue and red/green opsins (white), Cfh (red), and a DAPI nuclear stain (blue) showed a similar band of Cfh in the central region of the OS (green arrow; B ). Cone cells labeled with the opsin antibodies (green arrowheads) exhibit a polarized distribution of blue and red/green opsins (white) and Cfh (red). A coronal section from a male C57BL/6 eye double labeled with fluorescein conjugated WGA (green), which labels the matrix domains surrounding rod photoreceptors, and Cfh (red) showing Cfh within rod photoreceptor OS ( C ). The delineated area of interest is magnified and shown on the right. Cfh is clearly localized within the rods (arrows; D ). A coronal section from a male C57BL/6 eye labeled with fluorescein conjugated PNA (green), which binds cone matrix domains, and Cfh (red) showing Cfh is clearly localized within the cone cells (arrows; E ). The delineated area of interest is magnified and shown on the right ( F ). IS=photoreceptor inner segments; OS=outer segments; RPE=retinal pigment epithelium; WGA=wheat germ agglutinin; PNA=peanut agglutinin. Scale bar=20 μm.

    Journal: Molecular Vision

    Article Title: Localization of complement factor H gene expression and protein distribution in the mouse outer retina

    doi:

    Figure Lengend Snippet: Cfh localization within rod and cone photoreceptors. A sagittal section from a male BALB/c mouse eye immunolabeled for rhodopsin (green), Cfh (red), and a 4',6-diamidino-2-phenylindole (DAPI) nuclear stain (blue) showed only a localized band of Cfh in the central region of the OS (white arrow), but not throughout the OS. In addition, occasional Cfh labeling of cells believed to be cone cell IS was observed (white arrowheads; A ). A sagittal section from a male BALB/c mouse eye immunolabeled for blue and red/green opsins (white), Cfh (red), and a DAPI nuclear stain (blue) showed a similar band of Cfh in the central region of the OS (green arrow; B ). Cone cells labeled with the opsin antibodies (green arrowheads) exhibit a polarized distribution of blue and red/green opsins (white) and Cfh (red). A coronal section from a male C57BL/6 eye double labeled with fluorescein conjugated WGA (green), which labels the matrix domains surrounding rod photoreceptors, and Cfh (red) showing Cfh within rod photoreceptor OS ( C ). The delineated area of interest is magnified and shown on the right. Cfh is clearly localized within the rods (arrows; D ). A coronal section from a male C57BL/6 eye labeled with fluorescein conjugated PNA (green), which binds cone matrix domains, and Cfh (red) showing Cfh is clearly localized within the cone cells (arrows; E ). The delineated area of interest is magnified and shown on the right ( F ). IS=photoreceptor inner segments; OS=outer segments; RPE=retinal pigment epithelium; WGA=wheat germ agglutinin; PNA=peanut agglutinin. Scale bar=20 μm.

    Article Snippet: Double-labeling experiments for Cfh with either fluorescein conjugated wheat germ agglutinin (WGA) or peanut agglutinin (PNA; both from Vector Laboratories, catalog #FL-1021 and #FL-1071, respectively) were performed using eyes from male C57BL/6 mice.

    Techniques: Immunolabeling, Staining, Labeling