Convection:Article Title: Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.
Article Snippet: Trifluoroacetic acid (TFA; Sigma-Aldrich, cat. no. T6508) ! cautIon TFA is strongly corrosive and toxic. .. Triethylamine (Sigma-Aldrich, cat. no. T0886) Triethylene glycol (Sigma-Aldrich, cat. no. T59455) Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride, Sigma-Aldrich, cat. no. T6066) Trizma base (Sigma-Aldrich, cat. no. T1503) Tween 20 (Polysorbate 20; Sigma-Aldrich, cat. no. P2287) EQUIPMENT Amicon Ultra concentrators, 10-kDa cutoff (Millipore or equivalent) Bacterial shaker Bunsen burner Centrifuge (Eppendorf 5424 Series or equivalent) Centrifuge for spinning 250-ml bacterial cultures at 5,000g and spinning 50-ml tubes at 20,000g (Beckman or equivalent) Centrifuge tubes, 1.5 ml (Eppendorf or equivalent) Dropping funnel Equilibrating dropping funnel Erlenmeyer flasks Fast protein liquid chromatography (FPLC) instrument (DuoFlow system or similar; Bio-Rad) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Filter, 0.22 μm (Corning or equivalent) Fixed-height comb (Bio-Rad, cat. no. 170-4447) Freeze dryer Refrigerator Glass pipettes and Pasteur pipettes Gel permeation chromatography (GPC)–size-exclusion chromatography (SEC) was run at 50 °C in DMF (flow rate 1 ml/min) using a Spectra Physics Instruments apparatus equipped with a UV-detector SEC-3010 and a refractive index detector (SEC 3010) from WGE Dr. Bures. .. Columns: guard (7.5 × 75 mm), PolarGel-M (7.5 × 300 mm), calibration with linear polystyrene standards Direct Detect (Merck Millipore) Graduate cylinders Magnetic particle concentrator (Life Technologies, cat. no. 12002D or equivalent) Micropipettes (0.5–1,000 μl) and suitable tips Mini-PROTEAN tetra cell casting module (Bio-Rad, cat. no. 165-8021) Module (Bio-Rad, cat. no. 166-0827) NanoDrop 2000c UV-vis spectrophotometer (Thermo Scientific) Needle (21 gauge, 150 mm) NMR spectrometer (Varian Unity 400 or equivalent) Oil bath Parafilm Petri dishes, 100 × 20 mm (Sigma-Aldrich, cat. no. CLS430591 or equivalent) Pipette boy with serological pipette (5, 10 and 25 ml) Polypropylene conical tubes, 50 and 15 ml with graduation (Corning or equivalent) Rotary evaporator (Büchi or equivalent) Rotary mixer (RSM7, Ratek or equivalent) Schlenk line Separating funnel Septum Sub-cell GT UV-transparent wide mini-gel tray (cat. no. 170-4426) Syringes Test tube racks Vacuum line Water bath, 37 °C • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Box 3 | Synthesis of poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk ● tIMInG 7 d The alkyne-functionalized co-polymers poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk is required as one of the building blocks of the LbL capsules. procedure 1.
Fast Protein Liquid Chromatography:Article Title: Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.
Article Snippet: Trifluoroacetic acid (TFA; Sigma-Aldrich, cat. no. T6508) ! cautIon TFA is strongly corrosive and toxic. .. Triethylamine (Sigma-Aldrich, cat. no. T0886) Triethylene glycol (Sigma-Aldrich, cat. no. T59455) Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride, Sigma-Aldrich, cat. no. T6066) Trizma base (Sigma-Aldrich, cat. no. T1503) Tween 20 (Polysorbate 20; Sigma-Aldrich, cat. no. P2287) EQUIPMENT Amicon Ultra concentrators, 10-kDa cutoff (Millipore or equivalent) Bacterial shaker Bunsen burner Centrifuge (Eppendorf 5424 Series or equivalent) Centrifuge for spinning 250-ml bacterial cultures at 5,000g and spinning 50-ml tubes at 20,000g (Beckman or equivalent) Centrifuge tubes, 1.5 ml (Eppendorf or equivalent) Dropping funnel Equilibrating dropping funnel Erlenmeyer flasks Fast protein liquid chromatography (FPLC) instrument (DuoFlow system or similar; Bio-Rad) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Filter, 0.22 μm (Corning or equivalent) Fixed-height comb (Bio-Rad, cat. no. 170-4447) Freeze dryer Refrigerator Glass pipettes and Pasteur pipettes Gel permeation chromatography (GPC)–size-exclusion chromatography (SEC) was run at 50 °C in DMF (flow rate 1 ml/min) using a Spectra Physics Instruments apparatus equipped with a UV-detector SEC-3010 and a refractive index detector (SEC 3010) from WGE Dr. Bures. .. Columns: guard (7.5 × 75 mm), PolarGel-M (7.5 × 300 mm), calibration with linear polystyrene standards Direct Detect (Merck Millipore) Graduate cylinders Magnetic particle concentrator (Life Technologies, cat. no. 12002D or equivalent) Micropipettes (0.5–1,000 μl) and suitable tips Mini-PROTEAN tetra cell casting module (Bio-Rad, cat. no. 165-8021) Module (Bio-Rad, cat. no. 166-0827) NanoDrop 2000c UV-vis spectrophotometer (Thermo Scientific) Needle (21 gauge, 150 mm) NMR spectrometer (Varian Unity 400 or equivalent) Oil bath Parafilm Petri dishes, 100 × 20 mm (Sigma-Aldrich, cat. no. CLS430591 or equivalent) Pipette boy with serological pipette (5, 10 and 25 ml) Polypropylene conical tubes, 50 and 15 ml with graduation (Corning or equivalent) Rotary evaporator (Büchi or equivalent) Rotary mixer (RSM7, Ratek or equivalent) Schlenk line Separating funnel Septum Sub-cell GT UV-transparent wide mini-gel tray (cat. no. 170-4426) Syringes Test tube racks Vacuum line Water bath, 37 °C • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Box 3 | Synthesis of poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk ● tIMInG 7 d The alkyne-functionalized co-polymers poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk is required as one of the building blocks of the LbL capsules. procedure 1.
GPC Assay:Article Title: Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.
Article Snippet: Trifluoroacetic acid (TFA; Sigma-Aldrich, cat. no. T6508) ! cautIon TFA is strongly corrosive and toxic. .. Triethylamine (Sigma-Aldrich, cat. no. T0886) Triethylene glycol (Sigma-Aldrich, cat. no. T59455) Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride, Sigma-Aldrich, cat. no. T6066) Trizma base (Sigma-Aldrich, cat. no. T1503) Tween 20 (Polysorbate 20; Sigma-Aldrich, cat. no. P2287) EQUIPMENT Amicon Ultra concentrators, 10-kDa cutoff (Millipore or equivalent) Bacterial shaker Bunsen burner Centrifuge (Eppendorf 5424 Series or equivalent) Centrifuge for spinning 250-ml bacterial cultures at 5,000g and spinning 50-ml tubes at 20,000g (Beckman or equivalent) Centrifuge tubes, 1.5 ml (Eppendorf or equivalent) Dropping funnel Equilibrating dropping funnel Erlenmeyer flasks Fast protein liquid chromatography (FPLC) instrument (DuoFlow system or similar; Bio-Rad) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Filter, 0.22 μm (Corning or equivalent) Fixed-height comb (Bio-Rad, cat. no. 170-4447) Freeze dryer Refrigerator Glass pipettes and Pasteur pipettes Gel permeation chromatography (GPC)–size-exclusion chromatography (SEC) was run at 50 °C in DMF (flow rate 1 ml/min) using a Spectra Physics Instruments apparatus equipped with a UV-detector SEC-3010 and a refractive index detector (SEC 3010) from WGE Dr. Bures. .. Columns: guard (7.5 × 75 mm), PolarGel-M (7.5 × 300 mm), calibration with linear polystyrene standards Direct Detect (Merck Millipore) Graduate cylinders Magnetic particle concentrator (Life Technologies, cat. no. 12002D or equivalent) Micropipettes (0.5–1,000 μl) and suitable tips Mini-PROTEAN tetra cell casting module (Bio-Rad, cat. no. 165-8021) Module (Bio-Rad, cat. no. 166-0827) NanoDrop 2000c UV-vis spectrophotometer (Thermo Scientific) Needle (21 gauge, 150 mm) NMR spectrometer (Varian Unity 400 or equivalent) Oil bath Parafilm Petri dishes, 100 × 20 mm (Sigma-Aldrich, cat. no. CLS430591 or equivalent) Pipette boy with serological pipette (5, 10 and 25 ml) Polypropylene conical tubes, 50 and 15 ml with graduation (Corning or equivalent) Rotary evaporator (Büchi or equivalent) Rotary mixer (RSM7, Ratek or equivalent) Schlenk line Separating funnel Septum Sub-cell GT UV-transparent wide mini-gel tray (cat. no. 170-4426) Syringes Test tube racks Vacuum line Water bath, 37 °C • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Box 3 | Synthesis of poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk ● tIMInG 7 d The alkyne-functionalized co-polymers poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk is required as one of the building blocks of the LbL capsules. procedure 1.
Gel Permeation Chromatography:Article Title: Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.
Article Snippet: Trifluoroacetic acid (TFA; Sigma-Aldrich, cat. no. T6508) ! cautIon TFA is strongly corrosive and toxic. .. Triethylamine (Sigma-Aldrich, cat. no. T0886) Triethylene glycol (Sigma-Aldrich, cat. no. T59455) Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride, Sigma-Aldrich, cat. no. T6066) Trizma base (Sigma-Aldrich, cat. no. T1503) Tween 20 (Polysorbate 20; Sigma-Aldrich, cat. no. P2287) EQUIPMENT Amicon Ultra concentrators, 10-kDa cutoff (Millipore or equivalent) Bacterial shaker Bunsen burner Centrifuge (Eppendorf 5424 Series or equivalent) Centrifuge for spinning 250-ml bacterial cultures at 5,000g and spinning 50-ml tubes at 20,000g (Beckman or equivalent) Centrifuge tubes, 1.5 ml (Eppendorf or equivalent) Dropping funnel Equilibrating dropping funnel Erlenmeyer flasks Fast protein liquid chromatography (FPLC) instrument (DuoFlow system or similar; Bio-Rad) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Filter, 0.22 μm (Corning or equivalent) Fixed-height comb (Bio-Rad, cat. no. 170-4447) Freeze dryer Refrigerator Glass pipettes and Pasteur pipettes Gel permeation chromatography (GPC)–size-exclusion chromatography (SEC) was run at 50 °C in DMF (flow rate 1 ml/min) using a Spectra Physics Instruments apparatus equipped with a UV-detector SEC-3010 and a refractive index detector (SEC 3010) from WGE Dr. Bures. .. Columns: guard (7.5 × 75 mm), PolarGel-M (7.5 × 300 mm), calibration with linear polystyrene standards Direct Detect (Merck Millipore) Graduate cylinders Magnetic particle concentrator (Life Technologies, cat. no. 12002D or equivalent) Micropipettes (0.5–1,000 μl) and suitable tips Mini-PROTEAN tetra cell casting module (Bio-Rad, cat. no. 165-8021) Module (Bio-Rad, cat. no. 166-0827) NanoDrop 2000c UV-vis spectrophotometer (Thermo Scientific) Needle (21 gauge, 150 mm) NMR spectrometer (Varian Unity 400 or equivalent) Oil bath Parafilm Petri dishes, 100 × 20 mm (Sigma-Aldrich, cat. no. CLS430591 or equivalent) Pipette boy with serological pipette (5, 10 and 25 ml) Polypropylene conical tubes, 50 and 15 ml with graduation (Corning or equivalent) Rotary evaporator (Büchi or equivalent) Rotary mixer (RSM7, Ratek or equivalent) Schlenk line Separating funnel Septum Sub-cell GT UV-transparent wide mini-gel tray (cat. no. 170-4426) Syringes Test tube racks Vacuum line Water bath, 37 °C • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Box 3 | Synthesis of poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk ● tIMInG 7 d The alkyne-functionalized co-polymers poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk is required as one of the building blocks of the LbL capsules. procedure 1.
Chromatography:Article Title: Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.
Article Snippet: Trifluoroacetic acid (TFA; Sigma-Aldrich, cat. no. T6508) ! cautIon TFA is strongly corrosive and toxic. .. Triethylamine (Sigma-Aldrich, cat. no. T0886) Triethylene glycol (Sigma-Aldrich, cat. no. T59455) Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride, Sigma-Aldrich, cat. no. T6066) Trizma base (Sigma-Aldrich, cat. no. T1503) Tween 20 (Polysorbate 20; Sigma-Aldrich, cat. no. P2287) EQUIPMENT Amicon Ultra concentrators, 10-kDa cutoff (Millipore or equivalent) Bacterial shaker Bunsen burner Centrifuge (Eppendorf 5424 Series or equivalent) Centrifuge for spinning 250-ml bacterial cultures at 5,000g and spinning 50-ml tubes at 20,000g (Beckman or equivalent) Centrifuge tubes, 1.5 ml (Eppendorf or equivalent) Dropping funnel Equilibrating dropping funnel Erlenmeyer flasks Fast protein liquid chromatography (FPLC) instrument (DuoFlow system or similar; Bio-Rad) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Filter, 0.22 μm (Corning or equivalent) Fixed-height comb (Bio-Rad, cat. no. 170-4447) Freeze dryer Refrigerator Glass pipettes and Pasteur pipettes Gel permeation chromatography (GPC)–size-exclusion chromatography (SEC) was run at 50 °C in DMF (flow rate 1 ml/min) using a Spectra Physics Instruments apparatus equipped with a UV-detector SEC-3010 and a refractive index detector (SEC 3010) from WGE Dr. Bures. .. Columns: guard (7.5 × 75 mm), PolarGel-M (7.5 × 300 mm), calibration with linear polystyrene standards Direct Detect (Merck Millipore) Graduate cylinders Magnetic particle concentrator (Life Technologies, cat. no. 12002D or equivalent) Micropipettes (0.5–1,000 μl) and suitable tips Mini-PROTEAN tetra cell casting module (Bio-Rad, cat. no. 165-8021) Module (Bio-Rad, cat. no. 166-0827) NanoDrop 2000c UV-vis spectrophotometer (Thermo Scientific) Needle (21 gauge, 150 mm) NMR spectrometer (Varian Unity 400 or equivalent) Oil bath Parafilm Petri dishes, 100 × 20 mm (Sigma-Aldrich, cat. no. CLS430591 or equivalent) Pipette boy with serological pipette (5, 10 and 25 ml) Polypropylene conical tubes, 50 and 15 ml with graduation (Corning or equivalent) Rotary evaporator (Büchi or equivalent) Rotary mixer (RSM7, Ratek or equivalent) Schlenk line Separating funnel Septum Sub-cell GT UV-transparent wide mini-gel tray (cat. no. 170-4426) Syringes Test tube racks Vacuum line Water bath, 37 °C • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Box 3 | Synthesis of poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk ● tIMInG 7 d The alkyne-functionalized co-polymers poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk is required as one of the building blocks of the LbL capsules. procedure 1.
Refractive Index:Article Title: Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.
Article Snippet: Trifluoroacetic acid (TFA; Sigma-Aldrich, cat. no. T6508) ! cautIon TFA is strongly corrosive and toxic. .. Triethylamine (Sigma-Aldrich, cat. no. T0886) Triethylene glycol (Sigma-Aldrich, cat. no. T59455) Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride, Sigma-Aldrich, cat. no. T6066) Trizma base (Sigma-Aldrich, cat. no. T1503) Tween 20 (Polysorbate 20; Sigma-Aldrich, cat. no. P2287) EQUIPMENT Amicon Ultra concentrators, 10-kDa cutoff (Millipore or equivalent) Bacterial shaker Bunsen burner Centrifuge (Eppendorf 5424 Series or equivalent) Centrifuge for spinning 250-ml bacterial cultures at 5,000g and spinning 50-ml tubes at 20,000g (Beckman or equivalent) Centrifuge tubes, 1.5 ml (Eppendorf or equivalent) Dropping funnel Equilibrating dropping funnel Erlenmeyer flasks Fast protein liquid chromatography (FPLC) instrument (DuoFlow system or similar; Bio-Rad) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Filter, 0.22 μm (Corning or equivalent) Fixed-height comb (Bio-Rad, cat. no. 170-4447) Freeze dryer Refrigerator Glass pipettes and Pasteur pipettes Gel permeation chromatography (GPC)–size-exclusion chromatography (SEC) was run at 50 °C in DMF (flow rate 1 ml/min) using a Spectra Physics Instruments apparatus equipped with a UV-detector SEC-3010 and a refractive index detector (SEC 3010) from WGE Dr. Bures. .. Columns: guard (7.5 × 75 mm), PolarGel-M (7.5 × 300 mm), calibration with linear polystyrene standards Direct Detect (Merck Millipore) Graduate cylinders Magnetic particle concentrator (Life Technologies, cat. no. 12002D or equivalent) Micropipettes (0.5–1,000 μl) and suitable tips Mini-PROTEAN tetra cell casting module (Bio-Rad, cat. no. 165-8021) Module (Bio-Rad, cat. no. 166-0827) NanoDrop 2000c UV-vis spectrophotometer (Thermo Scientific) Needle (21 gauge, 150 mm) NMR spectrometer (Varian Unity 400 or equivalent) Oil bath Parafilm Petri dishes, 100 × 20 mm (Sigma-Aldrich, cat. no. CLS430591 or equivalent) Pipette boy with serological pipette (5, 10 and 25 ml) Polypropylene conical tubes, 50 and 15 ml with graduation (Corning or equivalent) Rotary evaporator (Büchi or equivalent) Rotary mixer (RSM7, Ratek or equivalent) Schlenk line Separating funnel Septum Sub-cell GT UV-transparent wide mini-gel tray (cat. no. 170-4426) Syringes Test tube racks Vacuum line Water bath, 37 °C • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Box 3 | Synthesis of poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk ● tIMInG 7 d The alkyne-functionalized co-polymers poly(N-vinyl pyrrolidone-ran-propargyl-acrylate) PVPONAlk is required as one of the building blocks of the LbL capsules. procedure 1.
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