Structured Review

Becton Dickinson fitc fibrinogen antibody
Fitc Fibrinogen Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc fibrinogen antibody/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fitc fibrinogen antibody - by Bioz Stars, 2022-09
86/100 stars

Images

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Becton Dickinson fluorescein isothiocyanate fitc labeled anti cd55 antibody
    <t>CD55</t> is expressed in the intimal lining layer of rheumatoid arthritis (RA) synovial tissue. (A) Sections of RA synovial tissue were stained with a fluorescein <t>isothiocyanate</t> <t>(FITC)-labeled</t> CD55 antibody (clone IA10) and analyzed by confocal microscopy. Note the fibrillar appearance of the fluorescent signal; magnification 20 x. (B) RA synovial tissue sections were stained with a CD55 antibody (clone 143–30) and visualized by immunohistochemistry and light microscopy; magnification 20 x. (C) In situ hybridization, using an antisense locked nucleic acid (LNA) oligomer, detects CD55 transcripts primarily in the synovial lining. Representative images are shown; magnification 10 x.
    Fluorescein Isothiocyanate Fitc Labeled Anti Cd55 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein isothiocyanate fitc labeled anti cd55 antibody/product/Becton Dickinson
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein isothiocyanate fitc labeled anti cd55 antibody - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    99
    Becton Dickinson flow cytometry fluorescein isothiocyanate fitc conjugated anti cd40l
    γ-secretase inhibitor DAPT reduces release of sBCMA and enhances surface expression of BCMA on activated human B cell. ( a , b ) Human B cells were differentiated into Ig-secreting cells via <t>CD40L+IL-21.</t> ( a ) Release of sBCMA on treatment with DAPT or TAPI-1 was measured using ELISA. sBCMA release was normalized to the amount of sBCMA shed under vehicle conditions, which was set as 100%. Combined data of three independent experiments (mean±s.e.m.). ( b ) These activated primary human B cells were subgrouped on the basis of expression of CD27 and CD38. A high surface expression of BCMA was seen on the CD27 ++ CD38 + subset. Surface expression of BCMA was enhanced using DAPT treatment (1 μM), while TAPI-I (50 μM) had little effect. ( c , d ) Human PBMCs were stimulated with R848+IL-2 for 7 days. ( c ) Release of sBCMA on treatment with DAPT or TAPI-1 was measured using ELISA. sBCMA release was normalized to the amount of sBCMA shed under vehicle conditions, which was set as 100%. Combined data of three independent experiments (mean±s.e.m.). ( d ) High surface expression of BCMA was seen on the CD19 + CD38 + subset; this was further enhanced by DAPT (1 μM), while TAPI-I (50 μM) had little effect.
    Flow Cytometry Fluorescein Isothiocyanate Fitc Conjugated Anti Cd40l, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry fluorescein isothiocyanate fitc conjugated anti cd40l/product/Becton Dickinson
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flow cytometry fluorescein isothiocyanate fitc conjugated anti cd40l - by Bioz Stars, 2022-09
    99/100 stars
      Buy from Supplier

    85
    Becton Dickinson interleukin 2 il 2 fluorescein isothiocyanate
    The functional T-cell response composition in young and older people appears to be very similar. Peripheral blood mononuclear cells (PBMCs) from cytomegalovirus (CMV)–positive donors were stimulated overnight with 19 CMV protein–derived overlapping peptide-pools. Activated T cells and their functional composition were analyzed by flow cytometry, including the activation markers, degranulation (CD107), CD154, <t>interleukin</t> 2 <t>(IL-2),</t> tumor necrosis factor (TNF), and interferon γ (IFN-γ). Responses from the United Kingdom cohort are shown for the 2 most frequently targeted proteins for each T-cell subset. A , Nonoverlapping subsets exhibiting 5, 4, 3, or 2 parallel functions or just 1 single function are shown as a proportion of the specifically activated T cells (in order of decreasing polyfunctionality). A significant difference between the age groups is observed for UL-83–specific CD4 + T cells with 2 functions, but, on the whole, subset distributions appear to be very similar. B , The functional dominance of each activation marker in a given response is assessed by the proportion of activated cells expressing it. Some variability between proteins was revealed, but there was no significant differences between the age groups. There were 5 end points evaluated, with the significance threshold set at a P value of ≤ .01. P values were determined by the Mann–Whitney test with Bonferroni correction for multiple end points.
    Interleukin 2 Il 2 Fluorescein Isothiocyanate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/interleukin 2 il 2 fluorescein isothiocyanate/product/Becton Dickinson
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    interleukin 2 il 2 fluorescein isothiocyanate - by Bioz Stars, 2022-09
    85/100 stars
      Buy from Supplier

    88
    Becton Dickinson cytokine antibodies
    Early acquisition of Salmonella Typhimurium (STm)–specific CD4 + T-cell immune responses. Percentage are shown for STm-specific CD4 + T cells producing total ( A ; n = 68), early ( B ; n = 36), and late ( C ; n = 32) <t>cytokine</t> and phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulated CD4 + T cells producing total cytokine ( D ; n = 62). Nonlinear polynomial regression models of third order were fit to data relating specific T-cell cytokine response to age. STm-specific T cells response within early and late periods was determined by linear regression, represented by solid central lines; dashed lines represent 95% confidence intervals.
    Cytokine Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine antibodies/product/Becton Dickinson
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine antibodies - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    CD55 is expressed in the intimal lining layer of rheumatoid arthritis (RA) synovial tissue. (A) Sections of RA synovial tissue were stained with a fluorescein isothiocyanate (FITC)-labeled CD55 antibody (clone IA10) and analyzed by confocal microscopy. Note the fibrillar appearance of the fluorescent signal; magnification 20 x. (B) RA synovial tissue sections were stained with a CD55 antibody (clone 143–30) and visualized by immunohistochemistry and light microscopy; magnification 20 x. (C) In situ hybridization, using an antisense locked nucleic acid (LNA) oligomer, detects CD55 transcripts primarily in the synovial lining. Representative images are shown; magnification 10 x.

    Journal: Arthritis Research & Therapy

    Article Title: CD55 deposited on synovial collagen fibers protects from immune complex-mediated arthritis

    doi: 10.1186/s13075-015-0518-4

    Figure Lengend Snippet: CD55 is expressed in the intimal lining layer of rheumatoid arthritis (RA) synovial tissue. (A) Sections of RA synovial tissue were stained with a fluorescein isothiocyanate (FITC)-labeled CD55 antibody (clone IA10) and analyzed by confocal microscopy. Note the fibrillar appearance of the fluorescent signal; magnification 20 x. (B) RA synovial tissue sections were stained with a CD55 antibody (clone 143–30) and visualized by immunohistochemistry and light microscopy; magnification 20 x. (C) In situ hybridization, using an antisense locked nucleic acid (LNA) oligomer, detects CD55 transcripts primarily in the synovial lining. Representative images are shown; magnification 10 x.

    Article Snippet: Sections were incubated with primary fluorescein isothiocyanate (FITC)-labeled anti-CD55 antibody (clone IA10; BD Biosciences) overnight at 4°C, washed, and analyzed by confocal microscopy.

    Techniques: Staining, Labeling, Confocal Microscopy, Immunohistochemistry, Light Microscopy, In Situ Hybridization

    γ-secretase inhibitor DAPT reduces release of sBCMA and enhances surface expression of BCMA on activated human B cell. ( a , b ) Human B cells were differentiated into Ig-secreting cells via CD40L+IL-21. ( a ) Release of sBCMA on treatment with DAPT or TAPI-1 was measured using ELISA. sBCMA release was normalized to the amount of sBCMA shed under vehicle conditions, which was set as 100%. Combined data of three independent experiments (mean±s.e.m.). ( b ) These activated primary human B cells were subgrouped on the basis of expression of CD27 and CD38. A high surface expression of BCMA was seen on the CD27 ++ CD38 + subset. Surface expression of BCMA was enhanced using DAPT treatment (1 μM), while TAPI-I (50 μM) had little effect. ( c , d ) Human PBMCs were stimulated with R848+IL-2 for 7 days. ( c ) Release of sBCMA on treatment with DAPT or TAPI-1 was measured using ELISA. sBCMA release was normalized to the amount of sBCMA shed under vehicle conditions, which was set as 100%. Combined data of three independent experiments (mean±s.e.m.). ( d ) High surface expression of BCMA was seen on the CD19 + CD38 + subset; this was further enhanced by DAPT (1 μM), while TAPI-I (50 μM) had little effect.

    Journal: Nature Communications

    Article Title: γ-secretase directly sheds the survival receptor BCMA from plasma cells

    doi: 10.1038/ncomms8333

    Figure Lengend Snippet: γ-secretase inhibitor DAPT reduces release of sBCMA and enhances surface expression of BCMA on activated human B cell. ( a , b ) Human B cells were differentiated into Ig-secreting cells via CD40L+IL-21. ( a ) Release of sBCMA on treatment with DAPT or TAPI-1 was measured using ELISA. sBCMA release was normalized to the amount of sBCMA shed under vehicle conditions, which was set as 100%. Combined data of three independent experiments (mean±s.e.m.). ( b ) These activated primary human B cells were subgrouped on the basis of expression of CD27 and CD38. A high surface expression of BCMA was seen on the CD27 ++ CD38 + subset. Surface expression of BCMA was enhanced using DAPT treatment (1 μM), while TAPI-I (50 μM) had little effect. ( c , d ) Human PBMCs were stimulated with R848+IL-2 for 7 days. ( c ) Release of sBCMA on treatment with DAPT or TAPI-1 was measured using ELISA. sBCMA release was normalized to the amount of sBCMA shed under vehicle conditions, which was set as 100%. Combined data of three independent experiments (mean±s.e.m.). ( d ) High surface expression of BCMA was seen on the CD19 + CD38 + subset; this was further enhanced by DAPT (1 μM), while TAPI-I (50 μM) had little effect.

    Article Snippet: The following monoclonal antibodies were used for surface expression analysis using flow cytometry: fluorescein isothiocyanate (FITC)-conjugated anti-CD40L (BD PharMingen, San Diego, CA), FITC-conjugated anti-CD138 (Diaclone, Besançon, France), Cy7-conjugated anti-CD27 and eFluor 450-conjugated anti-CD38 (EBioscience), Cy7-conjugated anti-CD19 (EBioscience).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay

    sBCMA is released when B cells differentiate towards plasma cells and comprises the extracellular domain plus part of the transmembranous region of BCMA. ( a ) Human purified B cells were activated for 5 days as indicated; IgG and sBCMA in the supernatant were quantified using ELISA. Combined data of three independent experiments (mean±s.e.m., P =0.0073, paired t -test). ( b ) PBMCs were stimulated with R848+IL-2 for 7 days. IgG and sBCMA in the supernatant were quantified using ELISA. Combined data of three independent experiments (mean±s.e.m., P =0.0227, paired t -test). ( c – e ) Human purified B cells were stimulated with CD40L+IL-21. ( c ) surface BCMA was measured using flow cytometry on unstimulated B cells, CD19 + CD38 − cells and CD19 + CD38 + cells. ( d ) Sorted CD38 + and CD38 − cells were cultured for another 24 h and the amount of shed sBCMA was measured using ELISA, combined data of two independent experiments. ( e ) Correlation between sBCMA release and surface expression of BCMA for a single replicate. ( f , g ) sBCMA was immunoprecipitated from supernatant of plasmacytoma cells ( f , lanes 1, 2), serum ( f , lane 3) and from supernatant of human purified B cells cultured with CD40L plus IL-21 ( f , lane 4) with anti-BCMA monoclonal antibodies (mAbs) A7D12.2 ( f , lanes 1 and 4) or C12A3.2 ( f , lane 2) or goat-anti-BCMA ( f , lane 3). Western blot analysis for BCMA ( f ) and silver staining of sBCMA immunoprecipitated from plasmacytoma supernatant ( g ) was performed. ( h ) The band at 6 kDa (from g ) and sBCMA obtained using immunoprecipitation were analysed with mass spectrometry. The aa sequences of BCMA and peptides identified after tryptic (blue) or chymotryptic (red) digestion are shown. No peptide was detected with a C-terminal aa that was not a site for either tryptic or chymotryptic cleavage, indicating that the precise cleavage site of γ-secretase within the membrane needs to be identified.

    Journal: Nature Communications

    Article Title: γ-secretase directly sheds the survival receptor BCMA from plasma cells

    doi: 10.1038/ncomms8333

    Figure Lengend Snippet: sBCMA is released when B cells differentiate towards plasma cells and comprises the extracellular domain plus part of the transmembranous region of BCMA. ( a ) Human purified B cells were activated for 5 days as indicated; IgG and sBCMA in the supernatant were quantified using ELISA. Combined data of three independent experiments (mean±s.e.m., P =0.0073, paired t -test). ( b ) PBMCs were stimulated with R848+IL-2 for 7 days. IgG and sBCMA in the supernatant were quantified using ELISA. Combined data of three independent experiments (mean±s.e.m., P =0.0227, paired t -test). ( c – e ) Human purified B cells were stimulated with CD40L+IL-21. ( c ) surface BCMA was measured using flow cytometry on unstimulated B cells, CD19 + CD38 − cells and CD19 + CD38 + cells. ( d ) Sorted CD38 + and CD38 − cells were cultured for another 24 h and the amount of shed sBCMA was measured using ELISA, combined data of two independent experiments. ( e ) Correlation between sBCMA release and surface expression of BCMA for a single replicate. ( f , g ) sBCMA was immunoprecipitated from supernatant of plasmacytoma cells ( f , lanes 1, 2), serum ( f , lane 3) and from supernatant of human purified B cells cultured with CD40L plus IL-21 ( f , lane 4) with anti-BCMA monoclonal antibodies (mAbs) A7D12.2 ( f , lanes 1 and 4) or C12A3.2 ( f , lane 2) or goat-anti-BCMA ( f , lane 3). Western blot analysis for BCMA ( f ) and silver staining of sBCMA immunoprecipitated from plasmacytoma supernatant ( g ) was performed. ( h ) The band at 6 kDa (from g ) and sBCMA obtained using immunoprecipitation were analysed with mass spectrometry. The aa sequences of BCMA and peptides identified after tryptic (blue) or chymotryptic (red) digestion are shown. No peptide was detected with a C-terminal aa that was not a site for either tryptic or chymotryptic cleavage, indicating that the precise cleavage site of γ-secretase within the membrane needs to be identified.

    Article Snippet: The following monoclonal antibodies were used for surface expression analysis using flow cytometry: fluorescein isothiocyanate (FITC)-conjugated anti-CD40L (BD PharMingen, San Diego, CA), FITC-conjugated anti-CD138 (Diaclone, Besançon, France), Cy7-conjugated anti-CD27 and eFluor 450-conjugated anti-CD38 (EBioscience), Cy7-conjugated anti-CD19 (EBioscience).

    Techniques: Purification, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cell Culture, Expressing, Immunoprecipitation, Western Blot, Silver Staining, Mass Spectrometry

    The functional T-cell response composition in young and older people appears to be very similar. Peripheral blood mononuclear cells (PBMCs) from cytomegalovirus (CMV)–positive donors were stimulated overnight with 19 CMV protein–derived overlapping peptide-pools. Activated T cells and their functional composition were analyzed by flow cytometry, including the activation markers, degranulation (CD107), CD154, interleukin 2 (IL-2), tumor necrosis factor (TNF), and interferon γ (IFN-γ). Responses from the United Kingdom cohort are shown for the 2 most frequently targeted proteins for each T-cell subset. A , Nonoverlapping subsets exhibiting 5, 4, 3, or 2 parallel functions or just 1 single function are shown as a proportion of the specifically activated T cells (in order of decreasing polyfunctionality). A significant difference between the age groups is observed for UL-83–specific CD4 + T cells with 2 functions, but, on the whole, subset distributions appear to be very similar. B , The functional dominance of each activation marker in a given response is assessed by the proportion of activated cells expressing it. Some variability between proteins was revealed, but there was no significant differences between the age groups. There were 5 end points evaluated, with the significance threshold set at a P value of ≤ .01. P values were determined by the Mann–Whitney test with Bonferroni correction for multiple end points.

    Journal: The Journal of Infectious Diseases

    Article Title: Functional Diversity of Cytomegalovirus–Specific T Cells Is Maintained in Older People and Significantly Associated With Protein Specificity and Response Size

    doi: 10.1093/infdis/jiw371

    Figure Lengend Snippet: The functional T-cell response composition in young and older people appears to be very similar. Peripheral blood mononuclear cells (PBMCs) from cytomegalovirus (CMV)–positive donors were stimulated overnight with 19 CMV protein–derived overlapping peptide-pools. Activated T cells and their functional composition were analyzed by flow cytometry, including the activation markers, degranulation (CD107), CD154, interleukin 2 (IL-2), tumor necrosis factor (TNF), and interferon γ (IFN-γ). Responses from the United Kingdom cohort are shown for the 2 most frequently targeted proteins for each T-cell subset. A , Nonoverlapping subsets exhibiting 5, 4, 3, or 2 parallel functions or just 1 single function are shown as a proportion of the specifically activated T cells (in order of decreasing polyfunctionality). A significant difference between the age groups is observed for UL-83–specific CD4 + T cells with 2 functions, but, on the whole, subset distributions appear to be very similar. B , The functional dominance of each activation marker in a given response is assessed by the proportion of activated cells expressing it. Some variability between proteins was revealed, but there was no significant differences between the age groups. There were 5 end points evaluated, with the significance threshold set at a P value of ≤ .01. P values were determined by the Mann–Whitney test with Bonferroni correction for multiple end points.

    Article Snippet: Antibodies We used the following fluorescence-conjugated monoclonal antibodies and staining reagents: anti-CD3-v500, anti-CD8-allophyocyanine (APC)-H7, anti-CD27-phycoerythrine (PE), interleukin 2 (IL-2)-fluorescein isothiocyanate (FITC), tumor necrosis factor α (TNF-α)-Alexa 700, and CD107a-APC (all BD Biosciences, Oxford, United Kingdom); anti-CD4-peridinin chlorophyll (PerCP), anti-interferon γ (IFN-γ) PE-cyanine 7, anti-CD154 Pacific-Blue (BioLegend, Cambridge, United Kingdom), anti-CD45RA-ECD (Beckman Coulter, United Kingdom), and Yellow live-dead stain (Invitrogen, Paisley, United Kingdom).

    Techniques: Functional Assay, Derivative Assay, Flow Cytometry, Cytometry, Activation Assay, Marker, Expressing, MANN-WHITNEY

    Early acquisition of Salmonella Typhimurium (STm)–specific CD4 + T-cell immune responses. Percentage are shown for STm-specific CD4 + T cells producing total ( A ; n = 68), early ( B ; n = 36), and late ( C ; n = 32) cytokine and phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulated CD4 + T cells producing total cytokine ( D ; n = 62). Nonlinear polynomial regression models of third order were fit to data relating specific T-cell cytokine response to age. STm-specific T cells response within early and late periods was determined by linear regression, represented by solid central lines; dashed lines represent 95% confidence intervals.

    Journal: The Journal of Infectious Diseases

    Article Title: Sequential Acquisition of T Cells and Antibodies to Nontyphoidal Salmonella in Malawian Children

    doi: 10.1093/infdis/jiu045

    Figure Lengend Snippet: Early acquisition of Salmonella Typhimurium (STm)–specific CD4 + T-cell immune responses. Percentage are shown for STm-specific CD4 + T cells producing total ( A ; n = 68), early ( B ; n = 36), and late ( C ; n = 32) cytokine and phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulated CD4 + T cells producing total cytokine ( D ; n = 62). Nonlinear polynomial regression models of third order were fit to data relating specific T-cell cytokine response to age. STm-specific T cells response within early and late periods was determined by linear regression, represented by solid central lines; dashed lines represent 95% confidence intervals.

    Article Snippet: Cells were washed with PBS/0.5% bovine serum albumin (BSA) buffer (Sigma Aldrich) and stained with 3 μL of surface antibodies (CD3-APC cyanine 7 and CD4-PB) and 5 μL of intracellular cytokine antibodies (IFN-γ–phycoerythrin, TNF-α–fluorescein isothiocyanate, and interleukin (IL) 2–APC [all Becton Dickson]).

    Techniques: