fitc conjugated concanavalin a  (Vector Laboratories)


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    Name:
    Unconjugated Concanavalin A Con A
    Description:
    Concanavalin A Con A recognizes α linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins At neutral and alkaline pH Con A exists as a tetramer of four identical subunits below pH 5 6 Con A dissociates into active dimers of 52 kDa Acetylation succinylation or other derivatizations can also produce stable forms with dimeric structures See succinylated Con A Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds Con A requires calcium or manganese ions at each of its four saccharide binding sites Although these divalent metal ions are bound tightly to the polypeptide structure buffers which can bind calcium such as phosphate generally should be avoided in diluting Con A since a gradual loss in activity may occur Inhibiting Eluting Sugar mixture of 200 mM α methylmannoside 200 mM α methylglucoside
    Catalog Number:
    L-1000
    Price:
    None
    Category:
    Proteins
    Size:
    500 mg
    Buy from Supplier


    Structured Review

    Vector Laboratories fitc conjugated concanavalin a
    Unconjugated Concanavalin A Con A
    Concanavalin A Con A recognizes α linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins At neutral and alkaline pH Con A exists as a tetramer of four identical subunits below pH 5 6 Con A dissociates into active dimers of 52 kDa Acetylation succinylation or other derivatizations can also produce stable forms with dimeric structures See succinylated Con A Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds Con A requires calcium or manganese ions at each of its four saccharide binding sites Although these divalent metal ions are bound tightly to the polypeptide structure buffers which can bind calcium such as phosphate generally should be avoided in diluting Con A since a gradual loss in activity may occur Inhibiting Eluting Sugar mixture of 200 mM α methylmannoside 200 mM α methylglucoside
    https://www.bioz.com/result/fitc conjugated concanavalin a/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated concanavalin a - by Bioz Stars, 2021-04
    95/100 stars

    Images

    1) Product Images from "Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Müller cells"

    Article Title: Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Müller cells

    Journal: Diabetologia

    doi: 10.1007/s00125-011-2081-0

    Decreased leucostasis in a Hif-1α KO mouse model of type 1 diabetes. a Non-diabetic mice, ( b ) diabetic wild-type (WT) mice and ( c ) diabetic Hif-1α KO mice at 2 months after onset of streptozotocin-induced diabetes were used for leucostasis assay. Adherent leucocytes (arrow) were stained with FITC-conjugated concanavalin-A in the retinal vasculature after removal of circulating leucocytes by perfusion. The retinal vasculature and leucocytes were visualised in retinal flat mounts under fluorescence microscope. Scale bar 50 μm. d Adherent leucocytes in the retinal vasculature were counted and averaged, showing that diabetic (DM) Hif-1α KO mice had significantly fewer leucocytes than diabetic WT mice. Values are mean±SEM; n =5, ** p
    Figure Legend Snippet: Decreased leucostasis in a Hif-1α KO mouse model of type 1 diabetes. a Non-diabetic mice, ( b ) diabetic wild-type (WT) mice and ( c ) diabetic Hif-1α KO mice at 2 months after onset of streptozotocin-induced diabetes were used for leucostasis assay. Adherent leucocytes (arrow) were stained with FITC-conjugated concanavalin-A in the retinal vasculature after removal of circulating leucocytes by perfusion. The retinal vasculature and leucocytes were visualised in retinal flat mounts under fluorescence microscope. Scale bar 50 μm. d Adherent leucocytes in the retinal vasculature were counted and averaged, showing that diabetic (DM) Hif-1α KO mice had significantly fewer leucocytes than diabetic WT mice. Values are mean±SEM; n =5, ** p

    Techniques Used: Mouse Assay, Staining, Fluorescence, Microscopy

    2) Product Images from "Adiponectin Suppresses Pathological Microvessel Formation in Retina Through Modulation of Tumor Necrosis Factor-α Expression"

    Article Title: Adiponectin Suppresses Pathological Microvessel Formation in Retina Through Modulation of Tumor Necrosis Factor-α Expression

    Journal: Circulation research

    doi: 10.1161/CIRCRESAHA.109.194506

    Exacerbated retinal leukostasis in APN-KO mice. A, Representative photographs of retinal vasculature and adherent leukocytes (arrows) labeled with FITC-conjugated Con A lectin in WT (left) and APN-KO (right) mice at P15. B, Quantitative analysis of leukocyte
    Figure Legend Snippet: Exacerbated retinal leukostasis in APN-KO mice. A, Representative photographs of retinal vasculature and adherent leukocytes (arrows) labeled with FITC-conjugated Con A lectin in WT (left) and APN-KO (right) mice at P15. B, Quantitative analysis of leukocyte

    Techniques Used: Mouse Assay, Labeling

    3) Product Images from "Antiangiogenic and Antineuroinflammatory Effects of Kallistatin Through Interactions With the Canonical Wnt Pathway"

    Article Title: Antiangiogenic and Antineuroinflammatory Effects of Kallistatin Through Interactions With the Canonical Wnt Pathway

    Journal: Diabetes

    doi: 10.2337/db12-1710

    Decreased retinal neuroinflammation and retinal vascular leakage in diabetic kallistatin-TG mice. Akita mice and Akita×kallistatin-TG mice at 5 months of age were used for leukostasis assay. A : Adherent leukocytes (arrow) were stained with FITC-conjugated concanavalin-A in the retinal vasculature. The retinal vasculature and leukocytes were visualized in retinal flat mounts under a fluorescence microscope. Scale bar: 50 μm. B : Adherent leukocytes in the retinal vasculature were counted in age-matched WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice. n = 7–10. C : Retinal levels of soluble ICAM-1 were measured by ELISA in age-matched WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice and expressed as percentages of the respective WT control. D : Retinal vascular permeability of WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice was measured using Evans blue as a tracer, normalized by retinal protein concentrations, and expressed as a percentage of the permeability in WT control. E : Retinal levels of VEGF-A were measured by ELISA in WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice and expressed as percentages of the respective WT control. All values are mean ± SD. * P
    Figure Legend Snippet: Decreased retinal neuroinflammation and retinal vascular leakage in diabetic kallistatin-TG mice. Akita mice and Akita×kallistatin-TG mice at 5 months of age were used for leukostasis assay. A : Adherent leukocytes (arrow) were stained with FITC-conjugated concanavalin-A in the retinal vasculature. The retinal vasculature and leukocytes were visualized in retinal flat mounts under a fluorescence microscope. Scale bar: 50 μm. B : Adherent leukocytes in the retinal vasculature were counted in age-matched WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice. n = 7–10. C : Retinal levels of soluble ICAM-1 were measured by ELISA in age-matched WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice and expressed as percentages of the respective WT control. D : Retinal vascular permeability of WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice was measured using Evans blue as a tracer, normalized by retinal protein concentrations, and expressed as a percentage of the permeability in WT control. E : Retinal levels of VEGF-A were measured by ELISA in WT, kallistatin-TG, Akita, and Akita×kallistatin-TG mice and expressed as percentages of the respective WT control. All values are mean ± SD. * P

    Techniques Used: Mouse Assay, Staining, Fluorescence, Microscopy, Enzyme-linked Immunosorbent Assay, Permeability

    4) Product Images from "PPARα activation directly upregulates thrombomodulin in the diabetic retina"

    Article Title: PPARα activation directly upregulates thrombomodulin in the diabetic retina

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-67579-1

    Pemafibrate inhibits retinal vascular leukostasis by upregulating TM. ( a ) Fluorescence microscopy images showing retinal adherent vascular leukocytes in diabetic rats. STZ-induced diabetic rats were treated with pemafibrate or vehicle and intravitreally injected with control siRNA or siRNA targeting Thbd . The retinal vascular adherent leukocytes were stained with FITC-conjugated concanavalin-A. Representative images of retinal flat mounts from nondiabetic rats (upper left), untreated diabetic rats (upper right), diabetic rats treated with pemafibrate and control siRNA (lower left), and diabetic rats treated with pemafibrate and si Thbd (lower right) are shown. Arrows indicate adherent leukocytes. Scale bar = 50 μm. ( b ) Quantification of adherent leukocytes in retinal images (n = 6 per group). (Results are expressed as mean ± SD. *p
    Figure Legend Snippet: Pemafibrate inhibits retinal vascular leukostasis by upregulating TM. ( a ) Fluorescence microscopy images showing retinal adherent vascular leukocytes in diabetic rats. STZ-induced diabetic rats were treated with pemafibrate or vehicle and intravitreally injected with control siRNA or siRNA targeting Thbd . The retinal vascular adherent leukocytes were stained with FITC-conjugated concanavalin-A. Representative images of retinal flat mounts from nondiabetic rats (upper left), untreated diabetic rats (upper right), diabetic rats treated with pemafibrate and control siRNA (lower left), and diabetic rats treated with pemafibrate and si Thbd (lower right) are shown. Arrows indicate adherent leukocytes. Scale bar = 50 μm. ( b ) Quantification of adherent leukocytes in retinal images (n = 6 per group). (Results are expressed as mean ± SD. *p

    Techniques Used: Fluorescence, Microscopy, Injection, Staining

    5) Product Images from "Therapeutic Effects of a Novel Agonist of Peroxisome Proliferator-Activated Receptor Alpha for the Treatment of Diabetic Retinopathy"

    Article Title: Therapeutic Effects of a Novel Agonist of Peroxisome Proliferator-Activated Receptor Alpha for the Treatment of Diabetic Retinopathy

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.16-21402

    Effects of Y-0452 on retinal vascular inflammation and leakage in diabetic rats. Rats with 1 month of STZ-induced diabetes received daily intraperitoneal injections of Y-0452 (10 mg/kg/d) or the same volume of the vehicle (Veh) as a control for 3 weeks. Retinal vascular endothelial cells and adherent leukocytes were stained with FITC–concanavalin-A and visualized under a fluorescence microscope. (A–C) Representative images of retinal adherent leukocytes in nondiabetic and STZ-induced diabetic rats ([A] nondiabetic, [B] STZ+Veh, [C] STZ+Y-0452). (D) Quantification of retinal adherent leukocytes was performed from ×40 magnification images. Adherent leukocytes were counted in six random fields per retina (white arrows indicate adherent leukocytes). (E) Retinal vascular leakage was measured using Evans blue as a tracer and normalized by total retinal protein concentrations (n = 6; *P
    Figure Legend Snippet: Effects of Y-0452 on retinal vascular inflammation and leakage in diabetic rats. Rats with 1 month of STZ-induced diabetes received daily intraperitoneal injections of Y-0452 (10 mg/kg/d) or the same volume of the vehicle (Veh) as a control for 3 weeks. Retinal vascular endothelial cells and adherent leukocytes were stained with FITC–concanavalin-A and visualized under a fluorescence microscope. (A–C) Representative images of retinal adherent leukocytes in nondiabetic and STZ-induced diabetic rats ([A] nondiabetic, [B] STZ+Veh, [C] STZ+Y-0452). (D) Quantification of retinal adherent leukocytes was performed from ×40 magnification images. Adherent leukocytes were counted in six random fields per retina (white arrows indicate adherent leukocytes). (E) Retinal vascular leakage was measured using Evans blue as a tracer and normalized by total retinal protein concentrations (n = 6; *P

    Techniques Used: Staining, Fluorescence, Microscopy

    6) Product Images from "Choroidal neovascularization is inhibited via an intraocular decrease of inflammatory cells in mice lacking complement component C3"

    Article Title: Choroidal neovascularization is inhibited via an intraocular decrease of inflammatory cells in mice lacking complement component C3

    Journal: Scientific Reports

    doi: 10.1038/srep15702

    Lesion size after laser photocoagulation. At 7 days after laser injury, the mice were perfused transcardially with PBS and then with FITC-conjugated concanavalin A (20 μg/mL in PBS) to label the choroidal neovascularization (CNVs). The eyes were removed and retinal pigment epithelium (RPE)-choroid flatmounts were prepared. Measurements of CNV lesion area were carried out using ImageJ. The outline of the CNV was drawn around the contour of the lesion and then the total lesion area was measured. The average area obtained from 4 lesions in each eye was used for analysis. ( A ) Lesion size was significantly smaller in C3 −/− mice than in wild-type (WT) mice. ( B ) The CNV in a representative WT mouse (white arrows). ( C ) The CNV lesion in a representative C3 −/− mouse is smaller than that in the WT mouse shown in B. Scale bars, 100 μm. * P
    Figure Legend Snippet: Lesion size after laser photocoagulation. At 7 days after laser injury, the mice were perfused transcardially with PBS and then with FITC-conjugated concanavalin A (20 μg/mL in PBS) to label the choroidal neovascularization (CNVs). The eyes were removed and retinal pigment epithelium (RPE)-choroid flatmounts were prepared. Measurements of CNV lesion area were carried out using ImageJ. The outline of the CNV was drawn around the contour of the lesion and then the total lesion area was measured. The average area obtained from 4 lesions in each eye was used for analysis. ( A ) Lesion size was significantly smaller in C3 −/− mice than in wild-type (WT) mice. ( B ) The CNV in a representative WT mouse (white arrows). ( C ) The CNV lesion in a representative C3 −/− mouse is smaller than that in the WT mouse shown in B. Scale bars, 100 μm. * P

    Techniques Used: Mouse Assay

    Related Articles

    Whole Genome Amplification:

    Article Title: Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
    Article Snippet: .. Lectins included concanavalin A (ConA), Galanthus nivalis lectin (GNL), peanut agglutinin (PNA), and wheat germ agglutinin (WGA), and were purchased from Vector Laboratories, Inc. (Burlingame, California, USA). ..

    Incubation:

    Article Title: The Metabolic Chemical Reporter 6‑Azido-6-deoxy-glucose Further Reveals the Substrate Promiscuity of O‑GlcNAc Transferase and Catalyzes the Discovery of Intracellular Protein Modification by O‑Glucose
    Article Snippet: .. The blots washed three times in TBST for 5 min and incubated with biotin-conjugated Concanavalin A (Vector Lab), diluted 1:1000 in TBST, for 1 h. The blot was then washed 3× with TBST for 10 min. Then the blot was then incubated with Strep-HRP at 1:1000 in blocking buffer for 1 h. After being washed 3× with TBST for 10 min, the blot was developed using ECL reagents. .. BL21(DE3) chemically competent E. coli (Novagen) were transformed with a pET24b plasmid encoding 6His-tagged ncOGT (nucleocytoplasmic OGT) by heat shock and plated on selective LB agar plates containing 50 μ g mL−1 kanamycin (LB-kan).

    Article Title: Glycan Profiling Shows Unvaried N-Glycomes in MSC Clones with Distinct Differentiation Potentials
    Article Snippet: .. Following 15 min incubation on ice, 120 μL of 10 μg/mL FITC-ConA (Vector Labs) was added to each sample. .. After two 15 min incubations interspersed with flicking of the tubes, 1 mL of washing buffer was added and the samples were centrifuged for 5 min at 450 g. The cell pellet was resuspended in 100 μL of washing buffer containing 1 μg/mL DAPI and incubated on ice in the dark for 5 min, followed by the addition of 1 mL washing buffer, and centrifugation for 5 min at 450 g. The pellet was resuspended in 400 μL PBS for flow cytometry.

    Blocking Assay:

    Article Title: The Metabolic Chemical Reporter 6‑Azido-6-deoxy-glucose Further Reveals the Substrate Promiscuity of O‑GlcNAc Transferase and Catalyzes the Discovery of Intracellular Protein Modification by O‑Glucose
    Article Snippet: .. The blots washed three times in TBST for 5 min and incubated with biotin-conjugated Concanavalin A (Vector Lab), diluted 1:1000 in TBST, for 1 h. The blot was then washed 3× with TBST for 10 min. Then the blot was then incubated with Strep-HRP at 1:1000 in blocking buffer for 1 h. After being washed 3× with TBST for 10 min, the blot was developed using ECL reagents. .. BL21(DE3) chemically competent E. coli (Novagen) were transformed with a pET24b plasmid encoding 6His-tagged ncOGT (nucleocytoplasmic OGT) by heat shock and plated on selective LB agar plates containing 50 μ g mL−1 kanamycin (LB-kan).

    Staining:

    Article Title: Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Müller cells
    Article Snippet: Briefly, mice were perfused through the left ventricle to remove circulating leucocytes in blood vessels. .. The adherent leucocytes in the vasculature were stained by perfusion with FITC-conjugated concanavalin-A (40 μg/ml; Vector Laboratories) and counted. .. The quantitative data were analysed and compared with those from wild-type mice using unpaired Student’s t test (two-tailed test).

    Labeling:

    Article Title: Arginase as a Mediator of Diabetic Retinopathy
    Article Snippet: The images were analyzed for reaction intensity by using the MetaMorph Image System (Molecular Devices). .. Leukocyte adhesion Retinal leukostasis was assayed by labeling the adherent leukocytes using Concanavalin A (Vector Laboratories). .. TUNEL assay Endothelial cell death was studied using TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) assay using Fluorescein in situ cell death detection kit (Millipore) according to the manufacturer’s protocol.

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  • 94
    Vector Laboratories fitc labeled con a
    Subcellular localization of Con A-binding molecule(s). (A1–9) Development of conjugation papillae and subcellular localization of <t>Con</t> A binding molecule(s) during development. Both mating type cells were separately pulse-labeled by Calcofluor White prior to the mixing of cells. Fluorescein isothiocyanate <t>(FITC)-labeled</t> Con A was added to the mixed cells just before pair formation (8 h after the mixing of cells). (A1–3) Nomarski images of three developmental stages of papilla formation. (A4–6) Calcofluor White staining images. Novel unstained papillae (arrowhead) expanded during development. (A7–9) Localization of FITC-Con A binding molecule(s). Red signals in each cell represent auto-fluorescence of chlorophyll. Scale bar: 20 μm. (B1–4) Localization of Con A binding molecule(s) on conjugation papillae. Calcofluor White and FITC-labeled Con A were simultaneously added to mixed cells extruding the conjugation papillae. (B1) Nomarski image of conjugation papillae. (B2) Fluorescence by Calcofluor White. (B3) Fluorescence by FITC-labeled Con A. (B4) Merged image of B2 and B3.
    Fitc Labeled Con A, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc labeled con a/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc labeled con a - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Subcellular localization of Con A-binding molecule(s). (A1–9) Development of conjugation papillae and subcellular localization of Con A binding molecule(s) during development. Both mating type cells were separately pulse-labeled by Calcofluor White prior to the mixing of cells. Fluorescein isothiocyanate (FITC)-labeled Con A was added to the mixed cells just before pair formation (8 h after the mixing of cells). (A1–3) Nomarski images of three developmental stages of papilla formation. (A4–6) Calcofluor White staining images. Novel unstained papillae (arrowhead) expanded during development. (A7–9) Localization of FITC-Con A binding molecule(s). Red signals in each cell represent auto-fluorescence of chlorophyll. Scale bar: 20 μm. (B1–4) Localization of Con A binding molecule(s) on conjugation papillae. Calcofluor White and FITC-labeled Con A were simultaneously added to mixed cells extruding the conjugation papillae. (B1) Nomarski image of conjugation papillae. (B2) Fluorescence by Calcofluor White. (B3) Fluorescence by FITC-labeled Con A. (B4) Merged image of B2 and B3.

    Journal: Frontiers in Plant Science

    Article Title: Concanavalin A Disrupts the Release of Fibrous Material Necessary for Zygote Formation of a Unicellular Charophycean Alga, Closterium peracerosum-strigosum-littorale Complex

    doi: 10.3389/fpls.2016.01040

    Figure Lengend Snippet: Subcellular localization of Con A-binding molecule(s). (A1–9) Development of conjugation papillae and subcellular localization of Con A binding molecule(s) during development. Both mating type cells were separately pulse-labeled by Calcofluor White prior to the mixing of cells. Fluorescein isothiocyanate (FITC)-labeled Con A was added to the mixed cells just before pair formation (8 h after the mixing of cells). (A1–3) Nomarski images of three developmental stages of papilla formation. (A4–6) Calcofluor White staining images. Novel unstained papillae (arrowhead) expanded during development. (A7–9) Localization of FITC-Con A binding molecule(s). Red signals in each cell represent auto-fluorescence of chlorophyll. Scale bar: 20 μm. (B1–4) Localization of Con A binding molecule(s) on conjugation papillae. Calcofluor White and FITC-labeled Con A were simultaneously added to mixed cells extruding the conjugation papillae. (B1) Nomarski image of conjugation papillae. (B2) Fluorescence by Calcofluor White. (B3) Fluorescence by FITC-labeled Con A. (B4) Merged image of B2 and B3.

    Article Snippet: Calcofluor White and FITC-labeled Con A were simultaneously added at the time of conjugation papilla formation.

    Techniques: Binding Assay, Conjugation Assay, Labeling, Staining, Fluorescence