Journal: Journal of Clinical Laboratory Analysis

Article Title: Correlation of integrin alpha 7 with clinicopathological characteristics and survival profiles, as well as its regulatory role in cell proliferation, apoptosis, and stemness in non‐small‐cell lung cancer

doi: 10.1002/jcla.22973

Figure Lengend Snippet: ITGA7 promoted cell proliferation, suppressed apoptosis, and enhanced stemness in A549 cells. A, ITGA7 mRNA expression after transfection. B, ITGA7 protein expression after transfection. C, Cell proliferation after transfection. D, E, Cell apoptosis after transfection. F, G, Proportion of CD133 + CD44 + cells after transfection. Comparison between two independent samples was determined by t test. P value < 0.05 was considered as significant. ITGA7, Integrin alpha 7

Article Snippet: Pre‐rinsed cells were, respectively, incubated with Alexa Fluor ® 647 conjugated CD133 Mouse mAb (Flow Specific) (1:50 dilution, CST) and FITC conjugated CD44 Rat mAb (1:100 dilution, CST) in incubation buffer at 37°C for an hour.

Techniques: Expressing, Transfection