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Scheme 1. The schematic depicts the steps required in A. <t>veronii</t> sensing. The approach begins by assessing the peroxidase-mimic catalytic activity (NanoZyme) of AuNPs which transforms the TMB enzyme substrate into blue colored product (NanoZyme ON). After incubating the AuNPs with A. veronii aptamers, an aptamer- AuNPs conjugate (sensing probe) is generated, in which the aptamers adsorbed the surface of the AuNPs via noncovalent interactions, resulting in a loss of catalytic activity (NanoZyme OFF). Once A. veronii is exposed to the sensor probe, the aptamer’s strong affinity for A. veronii causes aptamer desorption from the AuNPs, resulting in catalytic activity recovery (NanoZyme ON). Nevertheless, no color appears when the sensor probe is exposed to other bacteria, suggesting that the aptamers do not desorb from the surface of the AuNPs (NanoZyme Off).
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Scheme 1. The schematic depicts the steps required in A. veronii sensing. The approach begins by assessing the peroxidase-mimic catalytic activity (NanoZyme) of AuNPs which transforms the TMB enzyme substrate into blue colored product (NanoZyme ON). After incubating the AuNPs with A. veronii aptamers, an aptamer- AuNPs conjugate (sensing probe) is generated, in which the aptamers adsorbed the surface of the AuNPs via noncovalent interactions, resulting in a loss of catalytic activity (NanoZyme OFF). Once A. veronii is exposed to the sensor probe, the aptamer’s strong affinity for A. veronii causes aptamer desorption from the AuNPs, resulting in catalytic activity recovery (NanoZyme ON). Nevertheless, no color appears when the sensor probe is exposed to other bacteria, suggesting that the aptamers do not desorb from the surface of the AuNPs (NanoZyme Off).

Journal: Biosensors and Bioelectronics X

Article Title: Aeromonas veronii specific aptamer and peroxidase mimic tyrosine-capped gold NanoZymes enable highly specific sensing of fish pathogenic bacteria

doi: 10.1016/j.biosx.2024.100505

Figure Lengend Snippet: Scheme 1. The schematic depicts the steps required in A. veronii sensing. The approach begins by assessing the peroxidase-mimic catalytic activity (NanoZyme) of AuNPs which transforms the TMB enzyme substrate into blue colored product (NanoZyme ON). After incubating the AuNPs with A. veronii aptamers, an aptamer- AuNPs conjugate (sensing probe) is generated, in which the aptamers adsorbed the surface of the AuNPs via noncovalent interactions, resulting in a loss of catalytic activity (NanoZyme OFF). Once A. veronii is exposed to the sensor probe, the aptamer’s strong affinity for A. veronii causes aptamer desorption from the AuNPs, resulting in catalytic activity recovery (NanoZyme ON). Nevertheless, no color appears when the sensor probe is exposed to other bacteria, suggesting that the aptamers do not desorb from the surface of the AuNPs (NanoZyme Off).

Article Snippet: The target fish pathogenic bacteria A. veronii (AV) and other bacterial pure cultures (A. hydrophila (AH), P. aeruginosa (PA), Klebsiella pneumoniae (KP), and Bacillus sp.) were isolated from diseased fish (Labeo rohita) collected from an aquaculture farm in West Bengal.

Techniques: Activity Assay, Generated, Bacteria

Fig. 4. Selectivity of the A. veronii sensor probe. (A) Colorimetric response recovery of sensor probe in the presence of A. veronii (108 CFU mL−1) (B) Sensor probe response in the presence of different bacteria (BA, Bacillus spp.; AV, A. veronii; AH, A. hydrophila; PA, P. aeruginosa; KP, K. pneumoniae) inset shows the corresponding optical images.

Journal: Biosensors and Bioelectronics X

Article Title: Aeromonas veronii specific aptamer and peroxidase mimic tyrosine-capped gold NanoZymes enable highly specific sensing of fish pathogenic bacteria

doi: 10.1016/j.biosx.2024.100505

Figure Lengend Snippet: Fig. 4. Selectivity of the A. veronii sensor probe. (A) Colorimetric response recovery of sensor probe in the presence of A. veronii (108 CFU mL−1) (B) Sensor probe response in the presence of different bacteria (BA, Bacillus spp.; AV, A. veronii; AH, A. hydrophila; PA, P. aeruginosa; KP, K. pneumoniae) inset shows the corresponding optical images.

Article Snippet: The target fish pathogenic bacteria A. veronii (AV) and other bacterial pure cultures (A. hydrophila (AH), P. aeruginosa (PA), Klebsiella pneumoniae (KP), and Bacillus sp.) were isolated from diseased fish (Labeo rohita) collected from an aquaculture farm in West Bengal.

Techniques: Bacteria

Fig. 5. Sensor probe response as a function of increasing A. veronii concen tration; Linear colorimetric response was observed in the range 103–108 CFU mL−1. The red line shows the linear fit of the data points.

Journal: Biosensors and Bioelectronics X

Article Title: Aeromonas veronii specific aptamer and peroxidase mimic tyrosine-capped gold NanoZymes enable highly specific sensing of fish pathogenic bacteria

doi: 10.1016/j.biosx.2024.100505

Figure Lengend Snippet: Fig. 5. Sensor probe response as a function of increasing A. veronii concen tration; Linear colorimetric response was observed in the range 103–108 CFU mL−1. The red line shows the linear fit of the data points.

Article Snippet: The target fish pathogenic bacteria A. veronii (AV) and other bacterial pure cultures (A. hydrophila (AH), P. aeruginosa (PA), Klebsiella pneumoniae (KP), and Bacillus sp.) were isolated from diseased fish (Labeo rohita) collected from an aquaculture farm in West Bengal.

Techniques: