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fis1  (Boster Bio)


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    Structured Review

    Boster Bio fis1
    Fis1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fis1/Anti-TTC11%2FFIS1+Antibody+Picoband/pm42002787-80-63-68
    Average 91 stars, based on 9 article reviews
    fis1 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Immunolabeling:

    Article Title: A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer’s Disease
    Article Snippet: .. Brain sections were immunolabeled with an antibody against OPA1 (1:200, cat # PB0773, Boster, Wuhan, China), MFN2 (1:100, Bioss, cat # bs-23685R, Beijing, China), DRP1 (1:200, Wanleibio, cat # WL03028, Shenyang, China), FIS1 (1:200, Boster, cat # A01932-2, Wuhan, China) and incubated with the corresponding secondary antibody. .. After washing, DAB (Boster, cat # AR1022, Wuhan, China) was used for dyeing, and hematoxylin was used for retaining.

    Incubation:

    Article Title: A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer’s Disease
    Article Snippet: .. Brain sections were immunolabeled with an antibody against OPA1 (1:200, cat # PB0773, Boster, Wuhan, China), MFN2 (1:100, Bioss, cat # bs-23685R, Beijing, China), DRP1 (1:200, Wanleibio, cat # WL03028, Shenyang, China), FIS1 (1:200, Boster, cat # A01932-2, Wuhan, China) and incubated with the corresponding secondary antibody. .. After washing, DAB (Boster, cat # AR1022, Wuhan, China) was used for dyeing, and hematoxylin was used for retaining.

    Article Title: Electroacupuncture improves motor function after ischemic stroke via regulating mitochondrial dynamics.
    Article Snippet: .. 10 % SDS-PAGE gel electrophoresis, PVDF membrane electrowetting transfer, 8 % milk sealed for 2 h, Tris buffered saline-tween (TBST) washing, adding the corresponding primary antibody: Mfn1 (Proteintech, 13798–1-AP), Mfn2 (Proteintech, 12186–1- AP), Fis1 (Boster, A01932–3), Drp1 (Proteintech, 12957–1-AP), Opa1 (Proteintech, 27733–1-AP), GAPDH (Proteintech, 60004–1-Ig), the membrane were incubated (with shaking) overnight at 4◦C. .. The corresponding goat anti-rabbit secondary antibody (Proteintech, PR30011, 1: 5000) or goat anti-mouse secondary antibody (Proteintech, PR30012, 1:5000), the membrane were incubated at room temperature for 1 h, and and then stained with ECL reagent.

    Software:

    Article Title: DMOG pretreatment restores osteogenic-adipogenic balance and mitochondrial function in ONFH BMSCs through the HIF-1α/Homer3 pathway.
    Article Snippet: .. Band intensity was quantified using ImageJ software.The primary antibodies used were Runt-related transcription factor 2 (RUNX2, 1:1000, Cat No. PB0171, Boster, China), and Osteopontin (OPN, 1:1000, Cat No. BM4208, Boster, China), and GAPDH (1:5000, Cat No. BM3874, Boster, China), β-Actin (1:50000, Cat No. AC026, ABclonal, China), and LC3B (1:2000, Cat No. BM4827, Boster, China), and DRP1 (1:2000, Cat No. A00556-2, Boster, China), and FIS1 (1:2000, Cat No. A01932-2, Boster, China), and OPA1 (1:2000, Cat No. PB0773, Boster, China), and MFN1 (1:2000, Cat No. PB0263, Boster, China), and HIF-1α (1:2000, Cat No. 20960-1-AP, Proteintech, China), and Homer3(1:1000, Cat No. 16624-1-AP, Proteintech, China). ..

    Pyrolysis Gas Chromatography:

    Article Title: A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer’s Disease
    Article Snippet: N 2 A cells were transfected with the Vector plasmid or plasmid-encoding APPswe or co-transfectioned with APPswe and pEnCMV / Ppargc1a plasmids for 48 h, cell homogenate was lysed and Western blots were performed as described previously [ ]. .. Primary antibodies against EGFP (1:1000, Beyotime, cat # AG281, Shanghai, China), Flag (1:1000, abm, cat # G188, Zhenjiang, China), OPA1 (1:1000, Boster, cat # PB0773, Wuhan, China), MFN1 (1:1000, Boster, cat # PB0263, Wuhan, China), MFN2 (1:800, Bioss, cat # bs-23685R, Beijing, China), DRP1 (1:1000, Wanleibio, cat # WL03028, Shenyang, China), FIS1 (1:1000, Boster, cat # A01932-2, Wuhan, China), BAX (1:5000, Abcam, cat # ab32503, Cambridge, MA, USA), Bcl-2 (1:2000, Abcam, cat # ab182858, Cambridge, MA, USA), PGC-1a (1:1000, Bioss, cat # bs-1832R, Beijing, China), KIF5A Flag (diluted 1:1000, Bioword, cat # BS71526, Nanjing, China), KIF5B (diluted 1:1000, Wanleibio, cat # WL04906, Shenyang, China), and the horseradish peroxidase-linked antibodies (1:5000, Beyotime, goat anti mouse IgG, cat # A0216 and goat anti rabbit IgG, cat # A0208, Shanghai, China) were used to probe these blots. .. Protein visualization was carried out using enhanced chemiluminescence (ECL, Beyotime Institute of Biotechnology, Shanghai, China).

    Control:

    Article Title: Short-Term DMOG treatment rejuvenates senescent mesenchymal stem cells by enhancing mitochondrial function and mitophagy through the HIF-1α/BNIP3 pathway
    Article Snippet: Total proteins were extracted from MSCs using RIPA lysis buffer (Boster, China), separated via SDS-PAGE, and transferred to PVDF membranes. .. The primary antibodies used were p53 (1:1000, BM4309, Boster, China), p21 (1:1000, BM3990, Boster, China), HIF-1α (1:1000, BM4083, Boster, China), MFN1 (1:1000, BM4882, Boster, China), MFN2 (1:1000, BM4906, Boster, China), FIS1 (1:1000, A01932-3, Boster, China), BNIP3 (1:1000, BA4304-2, Boster, China), and GAPDH (1:5000, BM1632, Boster, China) as an internal control. ..

    other:

    Article Title: Short-Term DMOG treatment rejuvenates senescent mesenchymal stem cells by enhancing mitochondrial function and mitophagy through the HIF-1α/BNIP3 pathway.
    Article Snippet: Total proteins were extracted from MSCs using RIPA lysis buffer (Boster, China), separated via SDS-PAGE, and transferred to PVDF membranes.

    SDS Page:

    Article Title: Electroacupuncture improves motor function after ischemic stroke via regulating mitochondrial dynamics.
    Article Snippet: .. 10 % SDS-PAGE gel electrophoresis, PVDF membrane electrowetting transfer, 8 % milk sealed for 2 h, Tris buffered saline-tween (TBST) washing, adding the corresponding primary antibody: Mfn1 (Proteintech, 13798–1-AP), Mfn2 (Proteintech, 12186–1- AP), Fis1 (Boster, A01932–3), Drp1 (Proteintech, 12957–1-AP), Opa1 (Proteintech, 27733–1-AP), GAPDH (Proteintech, 60004–1-Ig), the membrane were incubated (with shaking) overnight at 4◦C. .. The corresponding goat anti-rabbit secondary antibody (Proteintech, PR30011, 1: 5000) or goat anti-mouse secondary antibody (Proteintech, PR30012, 1:5000), the membrane were incubated at room temperature for 1 h, and and then stained with ECL reagent.

    Nucleic Acid Electrophoresis:

    Article Title: Electroacupuncture improves motor function after ischemic stroke via regulating mitochondrial dynamics.
    Article Snippet: .. 10 % SDS-PAGE gel electrophoresis, PVDF membrane electrowetting transfer, 8 % milk sealed for 2 h, Tris buffered saline-tween (TBST) washing, adding the corresponding primary antibody: Mfn1 (Proteintech, 13798–1-AP), Mfn2 (Proteintech, 12186–1- AP), Fis1 (Boster, A01932–3), Drp1 (Proteintech, 12957–1-AP), Opa1 (Proteintech, 27733–1-AP), GAPDH (Proteintech, 60004–1-Ig), the membrane were incubated (with shaking) overnight at 4◦C. .. The corresponding goat anti-rabbit secondary antibody (Proteintech, PR30011, 1: 5000) or goat anti-mouse secondary antibody (Proteintech, PR30012, 1:5000), the membrane were incubated at room temperature for 1 h, and and then stained with ECL reagent.

    Membrane:

    Article Title: Electroacupuncture improves motor function after ischemic stroke via regulating mitochondrial dynamics.
    Article Snippet: .. 10 % SDS-PAGE gel electrophoresis, PVDF membrane electrowetting transfer, 8 % milk sealed for 2 h, Tris buffered saline-tween (TBST) washing, adding the corresponding primary antibody: Mfn1 (Proteintech, 13798–1-AP), Mfn2 (Proteintech, 12186–1- AP), Fis1 (Boster, A01932–3), Drp1 (Proteintech, 12957–1-AP), Opa1 (Proteintech, 27733–1-AP), GAPDH (Proteintech, 60004–1-Ig), the membrane were incubated (with shaking) overnight at 4◦C. .. The corresponding goat anti-rabbit secondary antibody (Proteintech, PR30011, 1: 5000) or goat anti-mouse secondary antibody (Proteintech, PR30012, 1:5000), the membrane were incubated at room temperature for 1 h, and and then stained with ECL reagent.

    Saline:

    Article Title: Electroacupuncture improves motor function after ischemic stroke via regulating mitochondrial dynamics.
    Article Snippet: .. 10 % SDS-PAGE gel electrophoresis, PVDF membrane electrowetting transfer, 8 % milk sealed for 2 h, Tris buffered saline-tween (TBST) washing, adding the corresponding primary antibody: Mfn1 (Proteintech, 13798–1-AP), Mfn2 (Proteintech, 12186–1- AP), Fis1 (Boster, A01932–3), Drp1 (Proteintech, 12957–1-AP), Opa1 (Proteintech, 27733–1-AP), GAPDH (Proteintech, 60004–1-Ig), the membrane were incubated (with shaking) overnight at 4◦C. .. The corresponding goat anti-rabbit secondary antibody (Proteintech, PR30011, 1: 5000) or goat anti-mouse secondary antibody (Proteintech, PR30012, 1:5000), the membrane were incubated at room temperature for 1 h, and and then stained with ECL reagent.



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    COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes (Opa1, Mfn2, Drp1, and <t>Fis1)</t> and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes (Opa1, Mfn2, Drp1, and Fis1) and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation

    doi: 10.1016/j.mtbio.2026.102974

    Figure Lengend Snippet: COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes (Opa1, Mfn2, Drp1, and Fis1) and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: The following primary antibodies were used: Drp1 (CST, #8570, 1:1000, ∼80 kDa), Fis1 (Proteintech, 10956-1-AP, 1:1000, ∼17 kDa), Mfn1 (Abcam, ab104274, 1:1000, ∼84 kDa), Mfn2 (CST, #9482, 1:1000, ∼86 kDa), Opa1 (CST, #80471, 1:1000, ∼100–120 kDa), Pink1 (CST, #6946, 1:1000, ∼63 kDa), cGAS (CST, #15102, 1:1000, ∼60 kDa), STING (CST, #13647, 1:1000, ∼42–45 kDa), TBK1 (CST, #3013, 1:1000, ∼84 kDa), p-TBK1 (CST, #5483, 1:1000, ∼84 kDa), COX IV (Abcam, ab14744, 1:2000, ∼17 kDa), β-actin (Proteintech, 66009-1-Ig, 1:5000, ∼43 kDa), and GAPDH (Proteintech, 60004-1-Ig, 1:5000, After washing, the membranes were incubated with HRP-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse IgG, CST, 1:5000) for 1 h at room temperature.

    Techniques: Control, Fluorescence, Staining, Expressing, Western Blot, Microscopy