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TaKaRa first complementary dna cdna kit
First Complementary Dna Cdna Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Snippet: Afterward, 2 µg of total RNA was reverse-transcribed with a First complementary DNA (cDNA) kit (Takara Bio Inc., Japan).

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    TaKaRa strand cdna synthesis kit
    2-DG inhibits PEDV replication and gene expression. (A) Vero cells were treated with 5 mM 2-DG for the indicated time. Cell lysates were analyzed by Western blot using antibody against GRP78. (B) Vero cells were infected with PEDV (MOI = 0.01) after pretreated with 2-DG 24 h for indicated concentrations. Supernatant (virions) and cell lysates were analyzed by Western blot using antibody against PEDV N. (C) 2-DG affected PEDV replication. The cells were incubated either in the presence or absence of 2-DG for 24 h before infected with PEDV (MOI = 1). The cells were harvested for the indicated time for RT-PCR analysis using primers specific for PEDV N gene. The ratio was the gene copy number of N gene in 2-DG treated and mock-treated cells. (D) Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. PEDV titers in the supernatants were measured by plaque formation assays. (E, F) 5 × 10 5 Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. Total <t>RNA</t> was extracted, and <t>cDNA</t> was synthesized by reverse transcription. The RT-PCR was used to analyze the viral RNA copy numbers in the cells and supernatants (virion).
    Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strand cdna synthesis kit/product/TaKaRa
    Average 99 stars, based on 227 article reviews
    Price from $9.99 to $1999.99
    strand cdna synthesis kit - by Bioz Stars, 2020-08
    99/100 stars
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    91
    TaKaRa first strand cdna kit
    Densin-180 protein is enriched in PSD fractions, and its <t>mRNA</t> expression is brain-specific. A , Enrichment of densin-180 protein in detergent-extracted PSD fractions. Immunoblots were prepared with 50 μg ( lanes 1 , 2 ) of rat brain homogenate ( Hom ) and synaptosome fractions ( Syn ) and 7.5 μg ( lanes 3–6 ) each of synaptosome ( Syn ), once Triton X-100-extracted PSD ( 1T ), twice Triton X-100-extracted PSD ( 2T ), and once Triton X-100 and then sarcosyl-extracted PSD ( 1T + S ). Densin-180 protein band ( arrow ) is visualized with antibody M2 against densin-180. Molecular weight markers and position of the dye front ( open arrowhead ) are shown at left . B , Densin-180 Northern blot. Poly(A) + RNA (5 μg) from 13 different tissue samples was electrophoresed on a 1% agarose gel. The mRNA was transferred to Zeta-Probe blotting membrane (Bio-Rad), and all lanes were determined to have equal amounts of RNA by methylene blue staining. Blots were probed with a random prime-labeled PCR-amplified DNA fragment of densin-180 spanning nucleotides 1100–2170 (specific activity, 10 9 cpm/μg). A single broad band at 7.4 kb was detected ( large arrow ) on autoradiographs exposed for 14 d with an intensification screen. The blot was then stripped and reprobed with the 2 kb random prime-labeled human β-actin <t>cDNA</t> (specific activity, 10 7 cpm/μg). The autoradiograph of an 8 hr exposure with an intensification screen is shown in the bottom panel . The two forms of β-actin message are indicated ( small arrows ).
    First Strand Cdna Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna kit/product/TaKaRa
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    first strand cdna kit - by Bioz Stars, 2020-08
    91/100 stars
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    99
    TaKaRa cdna synthesis kit
    Effects of DCA on mRNA and protein levels of OCTN2 in cancer cell lines. (A), analysis of mRNA levels of OCTN2 in DCA-treated cancer cell lines. After treatment with 0.5 and 1 μM DCA, total <t>RNA</t> in cancer cells was purified by TRIzol reagent for <t>cDNA</t> synthesis and the mRNA levels of OCTN2 were analyzed by Quantitative RT-PCR. (B), analysis of protein levels of OCTN2 in DCA-treated cancer cell lines. Total proteins were extracted and protein levels of OCTN2 were analyzed by western blotting. The relative expression of mRNA and protein was normalized to β-actin. Data presented represent the mean ± S.D. of three independent experiments. Significant difference from control group of 0.1% DMSO is denoted with asterisks (*, p
    Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis kit/product/TaKaRa
    Average 99 stars, based on 568 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis kit - by Bioz Stars, 2020-08
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    2-DG inhibits PEDV replication and gene expression. (A) Vero cells were treated with 5 mM 2-DG for the indicated time. Cell lysates were analyzed by Western blot using antibody against GRP78. (B) Vero cells were infected with PEDV (MOI = 0.01) after pretreated with 2-DG 24 h for indicated concentrations. Supernatant (virions) and cell lysates were analyzed by Western blot using antibody against PEDV N. (C) 2-DG affected PEDV replication. The cells were incubated either in the presence or absence of 2-DG for 24 h before infected with PEDV (MOI = 1). The cells were harvested for the indicated time for RT-PCR analysis using primers specific for PEDV N gene. The ratio was the gene copy number of N gene in 2-DG treated and mock-treated cells. (D) Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. PEDV titers in the supernatants were measured by plaque formation assays. (E, F) 5 × 10 5 Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. Total RNA was extracted, and cDNA was synthesized by reverse transcription. The RT-PCR was used to analyze the viral RNA copy numbers in the cells and supernatants (virion).

    Journal: Antiviral Research

    Article Title: Triggering unfolded protein response by 2-Deoxy-d-glucose inhibits porcine epidemic diarrhea virus propagation

    doi: 10.1016/j.antiviral.2014.03.007

    Figure Lengend Snippet: 2-DG inhibits PEDV replication and gene expression. (A) Vero cells were treated with 5 mM 2-DG for the indicated time. Cell lysates were analyzed by Western blot using antibody against GRP78. (B) Vero cells were infected with PEDV (MOI = 0.01) after pretreated with 2-DG 24 h for indicated concentrations. Supernatant (virions) and cell lysates were analyzed by Western blot using antibody against PEDV N. (C) 2-DG affected PEDV replication. The cells were incubated either in the presence or absence of 2-DG for 24 h before infected with PEDV (MOI = 1). The cells were harvested for the indicated time for RT-PCR analysis using primers specific for PEDV N gene. The ratio was the gene copy number of N gene in 2-DG treated and mock-treated cells. (D) Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. PEDV titers in the supernatants were measured by plaque formation assays. (E, F) 5 × 10 5 Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. Total RNA was extracted, and cDNA was synthesized by reverse transcription. The RT-PCR was used to analyze the viral RNA copy numbers in the cells and supernatants (virion).

    Article Snippet: For cDNA preparation, total RNA (1 μg) was reverse transcribed with first strand cDNA synthesis kit (Takara, China).

    Techniques: Expressing, Western Blot, Infection, Incubation, Reverse Transcription Polymerase Chain Reaction, Synthesized

    Basic characteristics of pJAB1. ( a ) The PCR result of the pJAB1 gene. M, 5000 bp DNA marker; 1, the pJAB1 gene; 2, blank control. ( b ) The exon–intron structure. Eight exons were found in the cDNA sequence. ( c ) The secondary structure showing chromo, MPN and DEAD domains in the pJAB1 protein. ( d ) Comparison of the nucleotide sequences of pJAB1 and hJAB1. Short bars represent the identical nucleotides among the two sequences. Different regions were indicated by lowercase letters

    Journal: Cell Death & Disease

    Article Title: Porcine JAB1 significantly enhances apoptosis induced by staurosporine

    doi: 10.1038/cddis.2013.357

    Figure Lengend Snippet: Basic characteristics of pJAB1. ( a ) The PCR result of the pJAB1 gene. M, 5000 bp DNA marker; 1, the pJAB1 gene; 2, blank control. ( b ) The exon–intron structure. Eight exons were found in the cDNA sequence. ( c ) The secondary structure showing chromo, MPN and DEAD domains in the pJAB1 protein. ( d ) Comparison of the nucleotide sequences of pJAB1 and hJAB1. Short bars represent the identical nucleotides among the two sequences. Different regions were indicated by lowercase letters

    Article Snippet: Extracted RNAs were subjected to RT-PCR using first-strand cDNA synthesis kit (Takara Bio., Dalian, China).

    Techniques: Polymerase Chain Reaction, Marker, Sequencing

    Densin-180 protein is enriched in PSD fractions, and its mRNA expression is brain-specific. A , Enrichment of densin-180 protein in detergent-extracted PSD fractions. Immunoblots were prepared with 50 μg ( lanes 1 , 2 ) of rat brain homogenate ( Hom ) and synaptosome fractions ( Syn ) and 7.5 μg ( lanes 3–6 ) each of synaptosome ( Syn ), once Triton X-100-extracted PSD ( 1T ), twice Triton X-100-extracted PSD ( 2T ), and once Triton X-100 and then sarcosyl-extracted PSD ( 1T + S ). Densin-180 protein band ( arrow ) is visualized with antibody M2 against densin-180. Molecular weight markers and position of the dye front ( open arrowhead ) are shown at left . B , Densin-180 Northern blot. Poly(A) + RNA (5 μg) from 13 different tissue samples was electrophoresed on a 1% agarose gel. The mRNA was transferred to Zeta-Probe blotting membrane (Bio-Rad), and all lanes were determined to have equal amounts of RNA by methylene blue staining. Blots were probed with a random prime-labeled PCR-amplified DNA fragment of densin-180 spanning nucleotides 1100–2170 (specific activity, 10 9 cpm/μg). A single broad band at 7.4 kb was detected ( large arrow ) on autoradiographs exposed for 14 d with an intensification screen. The blot was then stripped and reprobed with the 2 kb random prime-labeled human β-actin cDNA (specific activity, 10 7 cpm/μg). The autoradiograph of an 8 hr exposure with an intensification screen is shown in the bottom panel . The two forms of β-actin message are indicated ( small arrows ).

    Journal: The Journal of Neuroscience

    Article Title: Characterization of Densin-180, a New Brain-Specific Synaptic Protein of the O-Sialoglycoprotein Family

    doi: 10.1523/JNEUROSCI.16-21-06839.1996

    Figure Lengend Snippet: Densin-180 protein is enriched in PSD fractions, and its mRNA expression is brain-specific. A , Enrichment of densin-180 protein in detergent-extracted PSD fractions. Immunoblots were prepared with 50 μg ( lanes 1 , 2 ) of rat brain homogenate ( Hom ) and synaptosome fractions ( Syn ) and 7.5 μg ( lanes 3–6 ) each of synaptosome ( Syn ), once Triton X-100-extracted PSD ( 1T ), twice Triton X-100-extracted PSD ( 2T ), and once Triton X-100 and then sarcosyl-extracted PSD ( 1T + S ). Densin-180 protein band ( arrow ) is visualized with antibody M2 against densin-180. Molecular weight markers and position of the dye front ( open arrowhead ) are shown at left . B , Densin-180 Northern blot. Poly(A) + RNA (5 μg) from 13 different tissue samples was electrophoresed on a 1% agarose gel. The mRNA was transferred to Zeta-Probe blotting membrane (Bio-Rad), and all lanes were determined to have equal amounts of RNA by methylene blue staining. Blots were probed with a random prime-labeled PCR-amplified DNA fragment of densin-180 spanning nucleotides 1100–2170 (specific activity, 10 9 cpm/μg). A single broad band at 7.4 kb was detected ( large arrow ) on autoradiographs exposed for 14 d with an intensification screen. The blot was then stripped and reprobed with the 2 kb random prime-labeled human β-actin cDNA (specific activity, 10 7 cpm/μg). The autoradiograph of an 8 hr exposure with an intensification screen is shown in the bottom panel . The two forms of β-actin message are indicated ( small arrows ).

    Article Snippet: The cDNA was prepared from mRNA with the First Strand cDNA kit purchased from Clontech (Palo Alto, CA).

    Techniques: Expressing, Western Blot, Molecular Weight, Northern Blot, Agarose Gel Electrophoresis, Staining, Labeling, Polymerase Chain Reaction, Amplification, Activity Assay, Autoradiography

    Effects of DCA on mRNA and protein levels of OCTN2 in cancer cell lines. (A), analysis of mRNA levels of OCTN2 in DCA-treated cancer cell lines. After treatment with 0.5 and 1 μM DCA, total RNA in cancer cells was purified by TRIzol reagent for cDNA synthesis and the mRNA levels of OCTN2 were analyzed by Quantitative RT-PCR. (B), analysis of protein levels of OCTN2 in DCA-treated cancer cell lines. Total proteins were extracted and protein levels of OCTN2 were analyzed by western blotting. The relative expression of mRNA and protein was normalized to β-actin. Data presented represent the mean ± S.D. of three independent experiments. Significant difference from control group of 0.1% DMSO is denoted with asterisks (*, p

    Journal: PLoS ONE

    Article Title: Different Involvement of Promoter Methylation in the Expression of Organic Cation/Carnitine Transporter 2 (OCTN2) in Cancer Cell Lines

    doi: 10.1371/journal.pone.0076474

    Figure Lengend Snippet: Effects of DCA on mRNA and protein levels of OCTN2 in cancer cell lines. (A), analysis of mRNA levels of OCTN2 in DCA-treated cancer cell lines. After treatment with 0.5 and 1 μM DCA, total RNA in cancer cells was purified by TRIzol reagent for cDNA synthesis and the mRNA levels of OCTN2 were analyzed by Quantitative RT-PCR. (B), analysis of protein levels of OCTN2 in DCA-treated cancer cell lines. Total proteins were extracted and protein levels of OCTN2 were analyzed by western blotting. The relative expression of mRNA and protein was normalized to β-actin. Data presented represent the mean ± S.D. of three independent experiments. Significant difference from control group of 0.1% DMSO is denoted with asterisks (*, p

    Article Snippet: RNA purification, cDNA Synthesis and Quantitative Real-time PCR Total cellular RNA was isolated from the treated cells using TRIzol reagent. cDNA was synthesized from 1 μg of total RNA using the first cDNA synthesis kit (Takara, Dalian).

    Techniques: Purification, Quantitative RT-PCR, Western Blot, Expressing

    Expression of OCTN2 in cancer cell lines. (A), analysis of mRNA levels of OCTN2 in cancer cell lines. Total RNA in cancer cells was purified by TRIzol reagent for cDNA synthesis and the mRNA levels of OCTN2 were analyzed by Quantitative RT-PCR. (B), analysis of protein levels of OCTN2 in cancer cells. Total proteins were extracted and protein levels of OCTN2 were analyzed by western blotting. The relative expression of mRNA and protein was normalized to β-actin. The expression of HepG2 was set as 1. All of mRNA and protein analysis were performed three times. Significant difference from HepG2 or LS174T cell is denoted with asterisk (*, p

    Journal: PLoS ONE

    Article Title: Different Involvement of Promoter Methylation in the Expression of Organic Cation/Carnitine Transporter 2 (OCTN2) in Cancer Cell Lines

    doi: 10.1371/journal.pone.0076474

    Figure Lengend Snippet: Expression of OCTN2 in cancer cell lines. (A), analysis of mRNA levels of OCTN2 in cancer cell lines. Total RNA in cancer cells was purified by TRIzol reagent for cDNA synthesis and the mRNA levels of OCTN2 were analyzed by Quantitative RT-PCR. (B), analysis of protein levels of OCTN2 in cancer cells. Total proteins were extracted and protein levels of OCTN2 were analyzed by western blotting. The relative expression of mRNA and protein was normalized to β-actin. The expression of HepG2 was set as 1. All of mRNA and protein analysis were performed three times. Significant difference from HepG2 or LS174T cell is denoted with asterisk (*, p

    Article Snippet: RNA purification, cDNA Synthesis and Quantitative Real-time PCR Total cellular RNA was isolated from the treated cells using TRIzol reagent. cDNA was synthesized from 1 μg of total RNA using the first cDNA synthesis kit (Takara, Dalian).

    Techniques: Expressing, Purification, Quantitative RT-PCR, Western Blot