cdna synthesis  (Solis BioDyne)


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  • 99
    Name:
    FIREScript RT cDNA synthesis KIT
    Description:
    Genetically modified MMLV based reverse transcriptase with increased thermostability and improved performance at elevated temperatures
    Catalog Number:
    06-15-00050
    Price:
    None
    Category:
    cDNA synthesis
    Applications:
    cDNA synthesis, first strand cDNA synthesis, reverse transcription, gene expression analysis
    Size:
    50 rxn | 50 x 20 μl rxn
    Buy from Supplier


    Structured Review

    Solis BioDyne cdna synthesis
    FIREScript RT cDNA synthesis KIT
    Genetically modified MMLV based reverse transcriptase with increased thermostability and improved performance at elevated temperatures
    https://www.bioz.com/result/cdna synthesis/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2021-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
    Article Snippet: .. Stimulated blood samples were centrifuged at 400×g for 5 min and the supernatant was transferred to a new tube and spun at 4600×g for 10 min. Total RNA was extracted from 1 ml of the bottom fraction with the GeneJET RNA Purification Kit (Life Sciences, Thermo FishEr, Waltham, USA), quantified on a microplate spectrophotometer (Epoch, Biotek, Winooski, United States), and 50 ng was reverse transcribed using the FIREScript RT cDNA synthesis kit (Solis BioDyne, Tartu, Estonia). mRNA levels was analysed by RT-qPCR with a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, USA) as described previously , using HOT FIREPol EvaGreen qPCR Mix (Solis BioDyne, Tartu, Estonia) with a hot start at 95 °C followed by 40 cycles of 95 °C for 20 s, 58 °C for 40 s, and 72 °C for 20 s, followed by a melting curve analysis, using SYBR green fluorescence reading as described elsewhere . ..

    Spectrophotometry:

    Article Title: EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
    Article Snippet: .. Stimulated blood samples were centrifuged at 400×g for 5 min and the supernatant was transferred to a new tube and spun at 4600×g for 10 min. Total RNA was extracted from 1 ml of the bottom fraction with the GeneJET RNA Purification Kit (Life Sciences, Thermo FishEr, Waltham, USA), quantified on a microplate spectrophotometer (Epoch, Biotek, Winooski, United States), and 50 ng was reverse transcribed using the FIREScript RT cDNA synthesis kit (Solis BioDyne, Tartu, Estonia). mRNA levels was analysed by RT-qPCR with a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, USA) as described previously , using HOT FIREPol EvaGreen qPCR Mix (Solis BioDyne, Tartu, Estonia) with a hot start at 95 °C followed by 40 cycles of 95 °C for 20 s, 58 °C for 40 s, and 72 °C for 20 s, followed by a melting curve analysis, using SYBR green fluorescence reading as described elsewhere . ..

    Quantitative RT-PCR:

    Article Title: EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
    Article Snippet: .. Stimulated blood samples were centrifuged at 400×g for 5 min and the supernatant was transferred to a new tube and spun at 4600×g for 10 min. Total RNA was extracted from 1 ml of the bottom fraction with the GeneJET RNA Purification Kit (Life Sciences, Thermo FishEr, Waltham, USA), quantified on a microplate spectrophotometer (Epoch, Biotek, Winooski, United States), and 50 ng was reverse transcribed using the FIREScript RT cDNA synthesis kit (Solis BioDyne, Tartu, Estonia). mRNA levels was analysed by RT-qPCR with a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, USA) as described previously , using HOT FIREPol EvaGreen qPCR Mix (Solis BioDyne, Tartu, Estonia) with a hot start at 95 °C followed by 40 cycles of 95 °C for 20 s, 58 °C for 40 s, and 72 °C for 20 s, followed by a melting curve analysis, using SYBR green fluorescence reading as described elsewhere . ..

    Real-time Polymerase Chain Reaction:

    Article Title: EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
    Article Snippet: .. Stimulated blood samples were centrifuged at 400×g for 5 min and the supernatant was transferred to a new tube and spun at 4600×g for 10 min. Total RNA was extracted from 1 ml of the bottom fraction with the GeneJET RNA Purification Kit (Life Sciences, Thermo FishEr, Waltham, USA), quantified on a microplate spectrophotometer (Epoch, Biotek, Winooski, United States), and 50 ng was reverse transcribed using the FIREScript RT cDNA synthesis kit (Solis BioDyne, Tartu, Estonia). mRNA levels was analysed by RT-qPCR with a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, USA) as described previously , using HOT FIREPol EvaGreen qPCR Mix (Solis BioDyne, Tartu, Estonia) with a hot start at 95 °C followed by 40 cycles of 95 °C for 20 s, 58 °C for 40 s, and 72 °C for 20 s, followed by a melting curve analysis, using SYBR green fluorescence reading as described elsewhere . ..

    Article Title: RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle
    Article Snippet: .. The complementary DNA (cDNA) was reverse transcribed from 1 µg of total RNA using the FIREScript RT cDNA Synthesis Kit (Solis BioDyne, Tartu, Estonia), and qPCR was performed in the QuantStudio 5 Real-Time PCR System (Applied Biosciences, Foster City, USA). ..

    SYBR Green Assay:

    Article Title: EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
    Article Snippet: .. Stimulated blood samples were centrifuged at 400×g for 5 min and the supernatant was transferred to a new tube and spun at 4600×g for 10 min. Total RNA was extracted from 1 ml of the bottom fraction with the GeneJET RNA Purification Kit (Life Sciences, Thermo FishEr, Waltham, USA), quantified on a microplate spectrophotometer (Epoch, Biotek, Winooski, United States), and 50 ng was reverse transcribed using the FIREScript RT cDNA synthesis kit (Solis BioDyne, Tartu, Estonia). mRNA levels was analysed by RT-qPCR with a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, USA) as described previously , using HOT FIREPol EvaGreen qPCR Mix (Solis BioDyne, Tartu, Estonia) with a hot start at 95 °C followed by 40 cycles of 95 °C for 20 s, 58 °C for 40 s, and 72 °C for 20 s, followed by a melting curve analysis, using SYBR green fluorescence reading as described elsewhere . ..

    Fluorescence:

    Article Title: EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
    Article Snippet: .. Stimulated blood samples were centrifuged at 400×g for 5 min and the supernatant was transferred to a new tube and spun at 4600×g for 10 min. Total RNA was extracted from 1 ml of the bottom fraction with the GeneJET RNA Purification Kit (Life Sciences, Thermo FishEr, Waltham, USA), quantified on a microplate spectrophotometer (Epoch, Biotek, Winooski, United States), and 50 ng was reverse transcribed using the FIREScript RT cDNA synthesis kit (Solis BioDyne, Tartu, Estonia). mRNA levels was analysed by RT-qPCR with a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, USA) as described previously , using HOT FIREPol EvaGreen qPCR Mix (Solis BioDyne, Tartu, Estonia) with a hot start at 95 °C followed by 40 cycles of 95 °C for 20 s, 58 °C for 40 s, and 72 °C for 20 s, followed by a melting curve analysis, using SYBR green fluorescence reading as described elsewhere . ..

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  • 99
    Solis BioDyne cdna synthesis
    Cdna Synthesis, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

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