firepoldna polymerase firepol dna polymerase  (Solis BioDyne)


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    Solis BioDyne firepoldna polymerase firepol dna polymerase
    Threshold value of the NPCR assay for C. pneumoniae detection in sputa. Lines: M λ/ Pst I size marker; 500 µL of negative sputum was spiked with 1 µL of 10 −4 –10 1 dilution of original culture C. pneumoniae TWAR-183; <t>DNA</t> was isolated as described in the Materials and Methods section, and 0.5 µL was added to the first PCR reaction, amplified by external primers, and 1 µL was amplified in the second reaction; 10 −4 –10 1 serial dilutions; NC negative controls. ( A ) PNEU S/PNEU Nok; Chp up/Chp down primers. Size of PCR product is 461 bp. ( B ) PNEU S/Short down; Short up/a Chtinok primers. Size of PCR product is 121 bp.
    Firepoldna Polymerase Firepol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firepoldna polymerase firepol dna polymerase/product/Solis BioDyne
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    firepoldna polymerase firepol dna polymerase - by Bioz Stars, 2022-08
    96/100 stars

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    1) Product Images from "Reliable and Sensitive Nested PCR for the Detection of Chlamydia in Sputum"

    Article Title: Reliable and Sensitive Nested PCR for the Detection of Chlamydia in Sputum

    Journal: Microorganisms

    doi: 10.3390/microorganisms9050935

    Threshold value of the NPCR assay for C. pneumoniae detection in sputa. Lines: M λ/ Pst I size marker; 500 µL of negative sputum was spiked with 1 µL of 10 −4 –10 1 dilution of original culture C. pneumoniae TWAR-183; DNA was isolated as described in the Materials and Methods section, and 0.5 µL was added to the first PCR reaction, amplified by external primers, and 1 µL was amplified in the second reaction; 10 −4 –10 1 serial dilutions; NC negative controls. ( A ) PNEU S/PNEU Nok; Chp up/Chp down primers. Size of PCR product is 461 bp. ( B ) PNEU S/Short down; Short up/a Chtinok primers. Size of PCR product is 121 bp.
    Figure Legend Snippet: Threshold value of the NPCR assay for C. pneumoniae detection in sputa. Lines: M λ/ Pst I size marker; 500 µL of negative sputum was spiked with 1 µL of 10 −4 –10 1 dilution of original culture C. pneumoniae TWAR-183; DNA was isolated as described in the Materials and Methods section, and 0.5 µL was added to the first PCR reaction, amplified by external primers, and 1 µL was amplified in the second reaction; 10 −4 –10 1 serial dilutions; NC negative controls. ( A ) PNEU S/PNEU Nok; Chp up/Chp down primers. Size of PCR product is 461 bp. ( B ) PNEU S/Short down; Short up/a Chtinok primers. Size of PCR product is 121 bp.

    Techniques Used: Marker, Isolation, Polymerase Chain Reaction, Amplification

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  • 96
    Solis BioDyne firepoldna polymerase firepol dna polymerase
    Threshold value of the NPCR assay for C. pneumoniae detection in sputa. Lines: M λ/ Pst I size marker; 500 µL of negative sputum was spiked with 1 µL of 10 −4 –10 1 dilution of original culture C. pneumoniae TWAR-183; <t>DNA</t> was isolated as described in the Materials and Methods section, and 0.5 µL was added to the first PCR reaction, amplified by external primers, and 1 µL was amplified in the second reaction; 10 −4 –10 1 serial dilutions; NC negative controls. ( A ) PNEU S/PNEU Nok; Chp up/Chp down primers. Size of PCR product is 461 bp. ( B ) PNEU S/Short down; Short up/a Chtinok primers. Size of PCR product is 121 bp.
    Firepoldna Polymerase Firepol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firepoldna polymerase firepol dna polymerase/product/Solis BioDyne
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firepoldna polymerase firepol dna polymerase - by Bioz Stars, 2022-08
    96/100 stars
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    Threshold value of the NPCR assay for C. pneumoniae detection in sputa. Lines: M λ/ Pst I size marker; 500 µL of negative sputum was spiked with 1 µL of 10 −4 –10 1 dilution of original culture C. pneumoniae TWAR-183; DNA was isolated as described in the Materials and Methods section, and 0.5 µL was added to the first PCR reaction, amplified by external primers, and 1 µL was amplified in the second reaction; 10 −4 –10 1 serial dilutions; NC negative controls. ( A ) PNEU S/PNEU Nok; Chp up/Chp down primers. Size of PCR product is 461 bp. ( B ) PNEU S/Short down; Short up/a Chtinok primers. Size of PCR product is 121 bp.

    Journal: Microorganisms

    Article Title: Reliable and Sensitive Nested PCR for the Detection of Chlamydia in Sputum

    doi: 10.3390/microorganisms9050935

    Figure Lengend Snippet: Threshold value of the NPCR assay for C. pneumoniae detection in sputa. Lines: M λ/ Pst I size marker; 500 µL of negative sputum was spiked with 1 µL of 10 −4 –10 1 dilution of original culture C. pneumoniae TWAR-183; DNA was isolated as described in the Materials and Methods section, and 0.5 µL was added to the first PCR reaction, amplified by external primers, and 1 µL was amplified in the second reaction; 10 −4 –10 1 serial dilutions; NC negative controls. ( A ) PNEU S/PNEU Nok; Chp up/Chp down primers. Size of PCR product is 461 bp. ( B ) PNEU S/Short down; Short up/a Chtinok primers. Size of PCR product is 121 bp.

    Article Snippet: The DNA was amplified by FIREpolDNA polymerase FIREPol® DNA Polymerase (Solis BioDyne, Tartu, Estonia) in a 25 μL solution containing 1× buffer B, 0.2 mM dNTPs, 2.5 mM MgCl2, 1× S solution, 1 pmol/μL of each primer, 0.5 μL of DNA, and 0.04 U/μL of enzyme (461-bp amplicon).

    Techniques: Marker, Isolation, Polymerase Chain Reaction, Amplification