pcr  (Solis BioDyne)


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  • 99
    Name:
    HOT FIREPol DNA Polymerase
    Description:
    A chemically modified hot start version of the thermostable Taq DNA polymerase FIREPol This enzyme is activated only by pre incubation at 95°C preventing any unspecific polymerase activity at lower temperatures during reaction set up Increased specificity and sensitivity Reduced primer dimer formation Reaction set up and shipment without ice
    Catalog Number:
    01-02-00500
    Price:
    None
    Category:
    Endpoint PCR
    Applications:
    PCR, qPCR, HOT-start, cloning, sanger sequencing
    Size:
    500 U | 100 µl
    Buy from Supplier


    Structured Review

    Solis BioDyne pcr
    HOT FIREPol DNA Polymerase
    A chemically modified hot start version of the thermostable Taq DNA polymerase FIREPol This enzyme is activated only by pre incubation at 95°C preventing any unspecific polymerase activity at lower temperatures during reaction set up Increased specificity and sensitivity Reduced primer dimer formation Reaction set up and shipment without ice
    https://www.bioz.com/result/pcr/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2021-09
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Expression of Enzymes Associated with Prostaglandin Synthesis in Equine Conceptuses
    Article Snippet: .. The qRT-PCR was done in 20 µL reactions including 20 ng cDNA, 0.2 mM of each dNTP, 3 mM MgCl2 , 1× buffer B2 (Solis BioDyne, Tartu, Estonia), 300 nM of each primer, 200 nM probe, 50 nM ROX reference dye (Biotium, Hayward, CA, USA) and 1 unit of HOT FIREPol DNA polymerase (Solis BioDyne). ..

    Multiplex Assay:

    Article Title: Epigenetic Changes in Equine Embryos after Short-Term Storage at Different Temperatures
    Article Snippet: .. The multiplex PCR was performed in 25 µL reaction volumes including 200 µM of each dNTP, 1 × buffer B2 (Solis BioDyne, Tartu, Estonia), 3 mM MgCl2 , 120 nM of each outer primer, 1.2 units HOT FIREPol DNA polymerase (Solis BioDyne, Tartu, Estonia) and 2 µL bisufite-treated DNA. ..

    Polymerase Chain Reaction:

    Article Title: Epigenetic Changes in Equine Embryos after Short-Term Storage at Different Temperatures
    Article Snippet: .. The multiplex PCR was performed in 25 µL reaction volumes including 200 µM of each dNTP, 1 × buffer B2 (Solis BioDyne, Tartu, Estonia), 3 mM MgCl2 , 120 nM of each outer primer, 1.2 units HOT FIREPol DNA polymerase (Solis BioDyne, Tartu, Estonia) and 2 µL bisufite-treated DNA. ..

    Article Title: The Foster method: Rapid and non-invasive detection of clinically relevant American Foulbrood disease levels using eDNA sampling and a dual-target qPCR assay, with its potential for other hive pathogens
    Article Snippet: .. Conventional PCRs used the same 2 μL each DNA dilution in a 20 μL PCR using HOT FIREPol polymerase, ready to load (Solis BioDyne, Tartu, Estonia) and 0.3 μM each primer. ..

    Article Title: Single nucleotide polymorphism rs5029937 in TNFAIP3 gene is correlated with risk of rheumatoid arthritis
    Article Snippet: .. HRM was performed using HOT FIREPol EvaGreen HRM Mix (no ROX) HRM PCR kit which contains HOT FIREPol® DNA Polymerase, 5x EvaGreen® HRM buffer, 12.5 mM MgCl2, dNTPs, Bovine serum albumin (BSA), and EvaGreen dye (Solis BioDyne Estonia). ..

    Transfection:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    CRISPR:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    Amplification:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    Generated:

    Article Title: The Role of Small Molecules and Their Effect on the Molecular Mechanisms of Early Retinal Organoid Development
    Article Snippet: .. Total RNA was extracted from 5 to 6 organoids per sample using the Qiagen RNeasy Mini kit with a DNase step according to the manufacturer’s instructions. cDNA was generated using Superscript III (Invitrogen, Waltham, MA, USA), and sq-PCR was performed with HOT FIREpol DNA Polymerase (Solis Biodyne, Tartu, Estonia). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. XBP1 splicing was detected by RT-PCR using HOT FIREPol DNA Polymerase (Solis BioDyne) and primers that span the IRE1-mediated splicing event on the XBP1 mRNA ( ). ..

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  • 98
    Solis BioDyne fire pol dna polymerase
    Results of 27 <t>LR-PCR</t> amplifications for one patient. Line 1 - ladder λ <t>DNA</t> Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.
    Fire Pol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fire pol dna polymerase/product/Solis BioDyne
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fire pol dna polymerase - by Bioz Stars, 2021-09
    98/100 stars
      Buy from Supplier

    99
    Solis BioDyne pcr
    Results of 27 <t>LR-PCR</t> amplifications for one patient. Line 1 - ladder λ <t>DNA</t> Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.
    Pcr, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Results of 27 LR-PCR amplifications for one patient. Line 1 - ladder λ DNA Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.

    Journal: Scientific Reports

    Article Title: Longrange PCR-based next-generation sequencing in pharmacokinetics and pharmacodynamics study of propofol among patients under general anaesthesia

    doi: 10.1038/s41598-017-15657-2

    Figure Lengend Snippet: Results of 27 LR-PCR amplifications for one patient. Line 1 - ladder λ DNA Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.

    Article Snippet: After optimization, LR-PCR reactions were carried out on an Applied Biosystems 2720 Thermal Cycler (Applied Biosystems, Foster City, CA) with the use of one of three polymerase sets: GoTaq® Long PCR Master Mix (Promega, Madison, USA), Long & High Fidelity PCR Enzyme Mix (BiotechRabbit, Hennigsdorf, Germany), Fire Pol® DNA Polymerase (Solis BioDyne, Tartu, Estonia) on the total volume of 30 μL, according to the manufacturer’s instructions (Table ).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Linear genomic comparison of sections within RDs . Comparisons are made with three sequences each using ACT (the Artemis Comparison Tool software release 5) over at least 30 kb: M. marinum M on top, M. ulcerans Agy99 at the bottom, and M. ulcerans of either haplotype, the Asian (RD2 and RD5) or the South American (RD9 and RD10) in the middle. Regions of sequence conformity are shown in parallel light grey plains, inverted DNA segments are depicted in dark grey and inverted surfaces, and white areas represent non-homologous regions like deletions and insertions. Some sequence displacements are visualized as grey areas displaying across the panels. Cut-off value for inclusion of sequence identity was 100 bp. The principal genetic backbone of the Asian and South American haplotypes (both members of the ancestral lineage) is identical for each alignment shown, but – as a matter of how the RDs were found – the particular excluded haplotypes reveal deletions in the respective RDs. Although showing the same genetic backbone as M. marinum in the marginal parts, the Mexican strains disclose large deletions over their respective RDs and are therefore not included in this computational analysis. The sequence regions were retrieved by scanning the contigs by PCR, and by cloning and sequencing of critical segments.

    Journal: BMC Evolutionary Biology

    Article Title: Evolution of two distinct phylogenetic lineages of the emerging human pathogen Mycobacterium ulcerans

    doi: 10.1186/1471-2148-7-177

    Figure Lengend Snippet: Linear genomic comparison of sections within RDs . Comparisons are made with three sequences each using ACT (the Artemis Comparison Tool software release 5) over at least 30 kb: M. marinum M on top, M. ulcerans Agy99 at the bottom, and M. ulcerans of either haplotype, the Asian (RD2 and RD5) or the South American (RD9 and RD10) in the middle. Regions of sequence conformity are shown in parallel light grey plains, inverted DNA segments are depicted in dark grey and inverted surfaces, and white areas represent non-homologous regions like deletions and insertions. Some sequence displacements are visualized as grey areas displaying across the panels. Cut-off value for inclusion of sequence identity was 100 bp. The principal genetic backbone of the Asian and South American haplotypes (both members of the ancestral lineage) is identical for each alignment shown, but – as a matter of how the RDs were found – the particular excluded haplotypes reveal deletions in the respective RDs. Although showing the same genetic backbone as M. marinum in the marginal parts, the Mexican strains disclose large deletions over their respective RDs and are therefore not included in this computational analysis. The sequence regions were retrieved by scanning the contigs by PCR, and by cloning and sequencing of critical segments.

    Article Snippet: DNA methods PCR was performed using FirePol 10× buffer and 0,5 μl FirePolTaq-Polymerase (Solis BioDyne, Tartu, Estonia), 2,5 ng genomic DNA, 0,6 μM forward and reverse primers each, 1,5 mM MgCl2 and 0,4 mM of each dNTP in a total volume of 25 μl.

    Techniques: Activated Clotting Time Assay, Software, Sequencing, Polymerase Chain Reaction, Clone Assay