finniqan ltq mass spectrometer  (Thermo Fisher)


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    Thermo Fisher finniqan ltq mass spectrometer
    Finniqan Ltq Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/finniqan ltq mass spectrometer/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    finniqan ltq mass spectrometer - by Bioz Stars, 2020-05
    91/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Shade-induced nuclear localization of PIF7 is regulated by phosphorylation and 14-3-3 proteins in Arabidopsis
    Article Snippet: .. The trypsin-digested peptides were concentrated and analyzed using a Finniqan LTQ mass spectrometer (Thermoquest, San Jose, USA) coupled with a surveyor HPLC system. .. Isolation of the nuclear fraction For nuclear fractionation, the different light-treated seedlings were ground to a fine powder and lysed with a buffer (20 mM Tris-HCl [pH 7.5], 20 mM KCl, 2 mM EDTA, 2.5 mM MgCl2 , 250 mM sucrose, 5 mM DTT, 25% glycerol and protease inhibitor cocktail) and filtered through Miracloth (Calbiochem, San Diego, USA).

    Article Title: A Strategy for Screening Monoclonal Antibodies for Arabidopsis Flowers
    Article Snippet: .. After digestion, the extracted peptides were analyzed by a Finniqan LTQ mass spectrometer (Thermo, USA) coupled with a surveyor HPLC system (Thermo, USA). ..

    Mass Spectrometry:

    Article Title: Shade-induced nuclear localization of PIF7 is regulated by phosphorylation and 14-3-3 proteins in Arabidopsis
    Article Snippet: .. The trypsin-digested peptides were concentrated and analyzed using a Finniqan LTQ mass spectrometer (Thermoquest, San Jose, USA) coupled with a surveyor HPLC system. .. Isolation of the nuclear fraction For nuclear fractionation, the different light-treated seedlings were ground to a fine powder and lysed with a buffer (20 mM Tris-HCl [pH 7.5], 20 mM KCl, 2 mM EDTA, 2.5 mM MgCl2 , 250 mM sucrose, 5 mM DTT, 25% glycerol and protease inhibitor cocktail) and filtered through Miracloth (Calbiochem, San Diego, USA).

    Article Title: A Strategy for Screening Monoclonal Antibodies for Arabidopsis Flowers
    Article Snippet: .. After digestion, the extracted peptides were analyzed by a Finniqan LTQ mass spectrometer (Thermo, USA) coupled with a surveyor HPLC system (Thermo, USA). ..

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    Thermo Fisher 2 d linear ion trap mass spectrometer
    Detection and identification of tumor-specific hTERT promoter-binding proteins Nuclear proteins were extracted from normal colon epithelial cell line (CCD 841 CoN) and colon cancer cell line (RKO), respectively. The hTERT promoter probe was incubated with the nuclear proteins and streptavidin-agarose beads to pull down DNA-bound proteins by centrifugation. hTERT promoter-binding proteins were separated by 12% SDS-PAGE and stained with Comassie brilliant blue. By comparing the gel lanes, significantly discrepant sections were identified, cut horizontally, and digested. The peptide mixture was extracted and analyzed by using an MDLC system coupled with a Thermo Finnigan <t>2-D</t> linear ion trap mass spectrometer. By peptide mapping using an internet-based proteomics tool, we identified the candidate tumor-specific hTERT promoter-binding protein as a transcription elongation factor, SPT5. The SDS-PAGE image shows the hTERT promoter-binding proteins. The arrow indicates the tumor cell-selective hTERT promoter-binding protein.
    2 D Linear Ion Trap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 d linear ion trap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    2 d linear ion trap mass spectrometer - by Bioz Stars, 2020-05
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    85
    Thermo Fisher 7t thermo finnigan ltq ft mass spectrometer
    Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of <t>Thermo-Finnigan</t> <t>LTQ</t> FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.
    7t Thermo Finnigan Ltq Ft Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7t thermo finnigan ltq ft mass spectrometer/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7t thermo finnigan ltq ft mass spectrometer - by Bioz Stars, 2020-05
    85/100 stars
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    93
    Thermo Fisher finnigan ltq ft ultra mass spectrometer
    Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of <t>Thermo-Finnigan</t> <t>LTQ</t> FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.
    Finnigan Ltq Ft Ultra Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/finnigan ltq ft ultra mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    finnigan ltq ft ultra mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars
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    Detection and identification of tumor-specific hTERT promoter-binding proteins Nuclear proteins were extracted from normal colon epithelial cell line (CCD 841 CoN) and colon cancer cell line (RKO), respectively. The hTERT promoter probe was incubated with the nuclear proteins and streptavidin-agarose beads to pull down DNA-bound proteins by centrifugation. hTERT promoter-binding proteins were separated by 12% SDS-PAGE and stained with Comassie brilliant blue. By comparing the gel lanes, significantly discrepant sections were identified, cut horizontally, and digested. The peptide mixture was extracted and analyzed by using an MDLC system coupled with a Thermo Finnigan 2-D linear ion trap mass spectrometer. By peptide mapping using an internet-based proteomics tool, we identified the candidate tumor-specific hTERT promoter-binding protein as a transcription elongation factor, SPT5. The SDS-PAGE image shows the hTERT promoter-binding proteins. The arrow indicates the tumor cell-selective hTERT promoter-binding protein.

    Journal: Oncotarget

    Article Title: Suppressor of Ty homolog-5, a novel tumor-specific human telomerase reverse transcriptase promoter-binding protein and activator in colon cancer cells

    doi:

    Figure Lengend Snippet: Detection and identification of tumor-specific hTERT promoter-binding proteins Nuclear proteins were extracted from normal colon epithelial cell line (CCD 841 CoN) and colon cancer cell line (RKO), respectively. The hTERT promoter probe was incubated with the nuclear proteins and streptavidin-agarose beads to pull down DNA-bound proteins by centrifugation. hTERT promoter-binding proteins were separated by 12% SDS-PAGE and stained with Comassie brilliant blue. By comparing the gel lanes, significantly discrepant sections were identified, cut horizontally, and digested. The peptide mixture was extracted and analyzed by using an MDLC system coupled with a Thermo Finnigan 2-D linear ion trap mass spectrometer. By peptide mapping using an internet-based proteomics tool, we identified the candidate tumor-specific hTERT promoter-binding protein as a transcription elongation factor, SPT5. The SDS-PAGE image shows the hTERT promoter-binding proteins. The arrow indicates the tumor cell-selective hTERT promoter-binding protein.

    Article Snippet: The peptide mixture from in-gel tryptic digestions was analyzed using an MDLC system (Michrom Bioresources Inc., Auburn, CA, USA) coupled with a Thermo Finnigan 2-D linear ion trap mass spectrometer (LTQXL , Thermo Inc., San Jose, CA, USA).

    Techniques: Binding Assay, Incubation, Centrifugation, SDS Page, Staining, Mass Spectrometry

    Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of Thermo-Finnigan LTQ FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Activated Ion Electron Capture Dissociation (AI ECD) of Proteins: Synchronization of Infrared and Electron Irradiation with Ion Magnetron Motion

    doi: 10.1016/j.jasms.2008.12.015

    Figure Lengend Snippet: Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of Thermo-Finnigan LTQ FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.

    Article Snippet: Mass Spectrometry Protein samples (1–3 μM in 49.5/49.5% of H2 O/CH3 OH and 1% of formic acid) were directly infused via an external nanospray ionization source (Advion Biosciences, Ithaca, NY) into a 7T Thermo Finnigan LTQ FT mass spectrometer (Thermo Fisher Scientific, Bremen, Germany).

    Techniques: