finnigan ltq mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher finnigan ltq mass spectrometer
    Finnigan Ltq Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/finnigan ltq mass spectrometer/product/Thermo Fisher
    Average 89 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    finnigan ltq mass spectrometer - by Bioz Stars, 2020-05
    89/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
    Article Snippet: .. For each run, a 1 μl aliquot of each 1μg/μl digest mixtures was injected into a Dionex Ultimate 3000 HPLC (Acclaim pepmap300 column, 150mm × 300um, C18, 5um, 300Å, Dionex, Sunnyvale, CA) and passed through a nanospray source into a Finnigan LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, MA; mass resolution 0.4 m/z). ..

    Injection:

    Article Title: Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
    Article Snippet: .. For each run, a 1 μl aliquot of each 1μg/μl digest mixtures was injected into a Dionex Ultimate 3000 HPLC (Acclaim pepmap300 column, 150mm × 300um, C18, 5um, 300Å, Dionex, Sunnyvale, CA) and passed through a nanospray source into a Finnigan LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, MA; mass resolution 0.4 m/z). ..

    Liquid Chromatography:

    Article Title: Circulating Modified Metabolites and a Risk of ESRD in Patients With Type 1 Diabetes and Chronic Kidney Disease
    Article Snippet: .. The liquid chromatography mass spectrometry portion of the platform incorporates a Waters ACQUITY UPLC system and a Finnigan LTQ mass spectrometer (Thermo Fisher Scientific), including an electrospray ionization source and linear ion trap mass analyzer. .. The gas chromatography column is 5% phenyl dimethyl silicone, and the temperature ramp is from 40°C to 300°C over 16 min. All samples were then analyzed on a Finnigan TRACE DSQ fast-scanning single-quadrupole mass spectrometer (Thermo Fisher Scientific) using electron impact ionization.

    Article Title: Synthesis of G-N2-(CH2)3-N2-G Trimethylene DNA interstrand cross-links
    Article Snippet: .. Analyze the cross-linked oligodeoxynucleotide by LC-ESI-MS on an Acquity ultraperformance liquid chromatography (UPLC) system (Waters Corp) interfaced to a Finnigan LTQ mass spectrometer (Thermo Scientific Corp, San Jose, CA) operating in the ESI negative ion mode and using an Acquity UPLC system BEH octadecysilane (C18) column (1.7 μm, 2.1 mm × 50 mm). ..

    Article Title: Translesion DNA Synthesis by Human DNA Polymerase ? on Templates Containing a Pyrimidopurinone Deoxyguanosine Adduct, 3-(2?-Deoxy-?-d-erythro-pentofuranosyl)pyrimido-[1,2-a]purin-10(3H)-one †
    Article Snippet: .. LC-ESI/MS/MS analysis was conducted on a Waters Acquity ultraperformance liquid chromatography system connected to a Finnigan LTQ mass spectrometer (ThermoElectron Corp., San Jose, CA). .. Negative ion electrospray ionization was used with a source voltage of 4 kV, source current 100 μA, auxiliary gas flow rate setting 20, sweep gas flow rate setting 5, sheath gas flow setting 34, and capillary voltage −90 V. Buffer A contained 10 mM NH4 CH3 CO2 plus 2% CH3 CN, and buffer B contained 10 mM NH4 CH3 CO plus 95% CH3 CN.

    Mass Spectrometry:

    Article Title: Identification of Glutathione Sulfotransferase-? (GSTP1) as a New Resveratrol Targeting Protein (RTP) and Studies of Resveratrol-responsive Protein Changes by Resveratrol Affinity Chromatography
    Article Snippet: .. The mass spectra were acquired using a Finnigan LTQ mass spectrometer (Thermo-Fisher, Waltham, MA, USA). .. The ms/ms spectra were analyzed by a thorough search of the genomic databases using the MASCOT search algorithm (Matrix Science Inc., Boston, MA, USA).

    Article Title: Circulating Modified Metabolites and a Risk of ESRD in Patients With Type 1 Diabetes and Chronic Kidney Disease
    Article Snippet: .. The liquid chromatography mass spectrometry portion of the platform incorporates a Waters ACQUITY UPLC system and a Finnigan LTQ mass spectrometer (Thermo Fisher Scientific), including an electrospray ionization source and linear ion trap mass analyzer. .. The gas chromatography column is 5% phenyl dimethyl silicone, and the temperature ramp is from 40°C to 300°C over 16 min. All samples were then analyzed on a Finnigan TRACE DSQ fast-scanning single-quadrupole mass spectrometer (Thermo Fisher Scientific) using electron impact ionization.

    Article Title: Synthesis of G-N2-(CH2)3-N2-G Trimethylene DNA interstrand cross-links
    Article Snippet: .. Analyze the cross-linked oligodeoxynucleotide by LC-ESI-MS on an Acquity ultraperformance liquid chromatography (UPLC) system (Waters Corp) interfaced to a Finnigan LTQ mass spectrometer (Thermo Scientific Corp, San Jose, CA) operating in the ESI negative ion mode and using an Acquity UPLC system BEH octadecysilane (C18) column (1.7 μm, 2.1 mm × 50 mm). ..

    Article Title: Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
    Article Snippet: .. For each run, a 1 μl aliquot of each 1μg/μl digest mixtures was injected into a Dionex Ultimate 3000 HPLC (Acclaim pepmap300 column, 150mm × 300um, C18, 5um, 300Å, Dionex, Sunnyvale, CA) and passed through a nanospray source into a Finnigan LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, MA; mass resolution 0.4 m/z). ..

    Article Title: iso-DGR Sequences Do Not Mediate Binding of Fibronectin N-terminal Modules to Adherent Fibronectin-null Fibroblasts *
    Article Snippet: .. Mass spectrometric analysis was performed on a ThermoElectron Finnigan LTQ mass spectrometer (Thermo Scientific) containing a linear ion trap and electrospray ionization source. .. The instrument was coupled with an Eksigent nano-liquid chromatography system (Eksigent, Dublin, CA).

    Article Title: Translesion DNA Synthesis by Human DNA Polymerase ? on Templates Containing a Pyrimidopurinone Deoxyguanosine Adduct, 3-(2?-Deoxy-?-d-erythro-pentofuranosyl)pyrimido-[1,2-a]purin-10(3H)-one †
    Article Snippet: .. LC-ESI/MS/MS analysis was conducted on a Waters Acquity ultraperformance liquid chromatography system connected to a Finnigan LTQ mass spectrometer (ThermoElectron Corp., San Jose, CA). .. Negative ion electrospray ionization was used with a source voltage of 4 kV, source current 100 μA, auxiliary gas flow rate setting 20, sweep gas flow rate setting 5, sheath gas flow setting 34, and capillary voltage −90 V. Buffer A contained 10 mM NH4 CH3 CO2 plus 2% CH3 CN, and buffer B contained 10 mM NH4 CH3 CO plus 95% CH3 CN.

    Article Title: Replication Past the N5-Methyl-Formamidopyrimidine Lesion of Deoxyguanosine by DNA Polymerases and an Improved Procedure for Sequence Analysis of In Vitro Bypass Products by Mass Spectrometry
    Article Snippet: .. MS analysis was performed in the Vanderbilt University facility on a Waters Acquity UPLC system (Waters, Milford, MA) connected to a Finnigan LTQ mass spectrometer (ThermoElectron) equipped with an Ion Max API source and a standard electrospray probe using an Acquity UPLC BEH C18 column (1 µm, 1.0 mm × 100 mm). ..

    Article Title: Structure-Function Relationships in Miscoding by Sulfolobus solfataricus DNA Polymerase Dpo4
    Article Snippet: .. LC-MS/MS analysis was performed using a Waters Acquity UPLC system (Waters, Milford, MA) connected to a Finnigan LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, MA) operating in the electrospray ionization negative ion mode. ..

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  • 99
    Thermo Fisher 2 d linear ion trap mass spectrometer
    Detection and identification of tumor-specific hTERT promoter-binding proteins Nuclear proteins were extracted from normal colon epithelial cell line (CCD 841 CoN) and colon cancer cell line (RKO), respectively. The hTERT promoter probe was incubated with the nuclear proteins and streptavidin-agarose beads to pull down DNA-bound proteins by centrifugation. hTERT promoter-binding proteins were separated by 12% SDS-PAGE and stained with Comassie brilliant blue. By comparing the gel lanes, significantly discrepant sections were identified, cut horizontally, and digested. The peptide mixture was extracted and analyzed by using an MDLC system coupled with a Thermo Finnigan <t>2-D</t> linear ion trap mass spectrometer. By peptide mapping using an internet-based proteomics tool, we identified the candidate tumor-specific hTERT promoter-binding protein as a transcription elongation factor, SPT5. The SDS-PAGE image shows the hTERT promoter-binding proteins. The arrow indicates the tumor cell-selective hTERT promoter-binding protein.
    2 D Linear Ion Trap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 d linear ion trap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    2 d linear ion trap mass spectrometer - by Bioz Stars, 2020-05
    99/100 stars
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    85
    Thermo Fisher 7t thermo finnigan ltq ft mass spectrometer
    Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of <t>Thermo-Finnigan</t> <t>LTQ</t> FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.
    7t Thermo Finnigan Ltq Ft Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7t thermo finnigan ltq ft mass spectrometer/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7t thermo finnigan ltq ft mass spectrometer - by Bioz Stars, 2020-05
    85/100 stars
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    93
    Thermo Fisher finnigan ltq ft ultra mass spectrometer
    Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of <t>Thermo-Finnigan</t> <t>LTQ</t> FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.
    Finnigan Ltq Ft Ultra Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/finnigan ltq ft ultra mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    finnigan ltq ft ultra mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Detection and identification of tumor-specific hTERT promoter-binding proteins Nuclear proteins were extracted from normal colon epithelial cell line (CCD 841 CoN) and colon cancer cell line (RKO), respectively. The hTERT promoter probe was incubated with the nuclear proteins and streptavidin-agarose beads to pull down DNA-bound proteins by centrifugation. hTERT promoter-binding proteins were separated by 12% SDS-PAGE and stained with Comassie brilliant blue. By comparing the gel lanes, significantly discrepant sections were identified, cut horizontally, and digested. The peptide mixture was extracted and analyzed by using an MDLC system coupled with a Thermo Finnigan 2-D linear ion trap mass spectrometer. By peptide mapping using an internet-based proteomics tool, we identified the candidate tumor-specific hTERT promoter-binding protein as a transcription elongation factor, SPT5. The SDS-PAGE image shows the hTERT promoter-binding proteins. The arrow indicates the tumor cell-selective hTERT promoter-binding protein.

    Journal: Oncotarget

    Article Title: Suppressor of Ty homolog-5, a novel tumor-specific human telomerase reverse transcriptase promoter-binding protein and activator in colon cancer cells

    doi:

    Figure Lengend Snippet: Detection and identification of tumor-specific hTERT promoter-binding proteins Nuclear proteins were extracted from normal colon epithelial cell line (CCD 841 CoN) and colon cancer cell line (RKO), respectively. The hTERT promoter probe was incubated with the nuclear proteins and streptavidin-agarose beads to pull down DNA-bound proteins by centrifugation. hTERT promoter-binding proteins were separated by 12% SDS-PAGE and stained with Comassie brilliant blue. By comparing the gel lanes, significantly discrepant sections were identified, cut horizontally, and digested. The peptide mixture was extracted and analyzed by using an MDLC system coupled with a Thermo Finnigan 2-D linear ion trap mass spectrometer. By peptide mapping using an internet-based proteomics tool, we identified the candidate tumor-specific hTERT promoter-binding protein as a transcription elongation factor, SPT5. The SDS-PAGE image shows the hTERT promoter-binding proteins. The arrow indicates the tumor cell-selective hTERT promoter-binding protein.

    Article Snippet: The peptide mixture from in-gel tryptic digestions was analyzed using an MDLC system (Michrom Bioresources Inc., Auburn, CA, USA) coupled with a Thermo Finnigan 2-D linear ion trap mass spectrometer (LTQXL , Thermo Inc., San Jose, CA, USA).

    Techniques: Binding Assay, Incubation, Centrifugation, SDS Page, Staining, Mass Spectrometry

    Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of Thermo-Finnigan LTQ FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Activated Ion Electron Capture Dissociation (AI ECD) of Proteins: Synchronization of Infrared and Electron Irradiation with Ion Magnetron Motion

    doi: 10.1016/j.jasms.2008.12.015

    Figure Lengend Snippet: Schematic representation of the geometrical arrangement for electron and IR laser beams in the ICR cell of Thermo-Finnigan LTQ FT. Top: overlap between ion trajectories, electron beam and IR beam in the plane A-A' at the entrance to the ICR cell.

    Article Snippet: Mass Spectrometry Protein samples (1–3 μM in 49.5/49.5% of H2 O/CH3 OH and 1% of formic acid) were directly infused via an external nanospray ionization source (Advion Biosciences, Ithaca, NY) into a 7T Thermo Finnigan LTQ FT mass spectrometer (Thermo Fisher Scientific, Bremen, Germany).

    Techniques: