Structured Review

Cayman Chemical filipin staining
Cholesterol accumulation in type II cells visualized with <t>filipin</t> after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P
Filipin Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/filipin staining/product/Cayman Chemical
Average 93 stars, based on 6 article reviews
Price from $9.99 to $1999.99
filipin staining - by Bioz Stars, 2020-07
93/100 stars

Images

1) Product Images from "Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung"

Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

doi: 10.1152/ajplung.00383.2011

Cholesterol accumulation in type II cells visualized with filipin after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P
Figure Legend Snippet: Cholesterol accumulation in type II cells visualized with filipin after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P

Techniques Used: Labeling, Quantitation Assay, Staining

Lamellar bodies were enriched with cholesterol after treatment with UA. Rat type II cells incubated with 10% FCS were untreated (Control) or treated with UA for 18 h. Cells were fixed and labeled with ABCA3 (gray, 1 and 5 ) and filipin (gray, 2 and 6 ). Merged image ( 3 and 7 ) of ABCA3 (green) and filipin (blue) and a merge with light ( 4 and 8 ) are shown. Outlines of cells are marked by white dashed lines. Two lamellar bodies ( 1′–4′ and 5′–8′ ) are highlighted for enlargement. ABCA3 ( 1′ and 5′ ), filipin ( 2′ and 6′ ), merged image ( 3′ and 7′ ), and merge with light ( 4′ and 8′ ) of enlarged lamellar bodies are shown. Scale bars, 5 μm.
Figure Legend Snippet: Lamellar bodies were enriched with cholesterol after treatment with UA. Rat type II cells incubated with 10% FCS were untreated (Control) or treated with UA for 18 h. Cells were fixed and labeled with ABCA3 (gray, 1 and 5 ) and filipin (gray, 2 and 6 ). Merged image ( 3 and 7 ) of ABCA3 (green) and filipin (blue) and a merge with light ( 4 and 8 ) are shown. Outlines of cells are marked by white dashed lines. Two lamellar bodies ( 1′–4′ and 5′–8′ ) are highlighted for enlargement. ABCA3 ( 1′ and 5′ ), filipin ( 2′ and 6′ ), merged image ( 3′ and 7′ ), and merge with light ( 4′ and 8′ ) of enlarged lamellar bodies are shown. Scale bars, 5 μm.

Techniques Used: Incubation, Labeling

Cholesterol accumulation in NPC1- and NPC2-positive lamellar bodies after treatment with compound UA. A : rat type II cells were treated with UA and triple-labeled with ABCA3 (green), filipin (gray or blue), and NPC1 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC1-positive/filipin-high lamellar body is shown in box 1 , and an NPC-1-positive/filipin-low lamellar body is shown in box 2 . B : type II cells treated with UA and triple-labeled with ABCA3 (green), filipin (gray), and NPC2 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC2-positive/filipin-high lamellar body is shown in box 1 , and a NPC-2-positive/filipin-low lamellar body is shown in box 2 . Scale bars, 5 μm.
Figure Legend Snippet: Cholesterol accumulation in NPC1- and NPC2-positive lamellar bodies after treatment with compound UA. A : rat type II cells were treated with UA and triple-labeled with ABCA3 (green), filipin (gray or blue), and NPC1 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC1-positive/filipin-high lamellar body is shown in box 1 , and an NPC-1-positive/filipin-low lamellar body is shown in box 2 . B : type II cells treated with UA and triple-labeled with ABCA3 (green), filipin (gray), and NPC2 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC2-positive/filipin-high lamellar body is shown in box 1 , and a NPC-2-positive/filipin-low lamellar body is shown in box 2 . Scale bars, 5 μm.

Techniques Used: Labeling

2) Product Images from "A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export"

Article Title: A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export

Journal: bioRxiv

doi: 10.1101/835686

Rab7 activation by the MCC GEF controls NPC1-dependent lysosomal cholesterol export. ( A, B ) Lysosomal cholesterol export is abolished in NPC1 -, C18orf8 -, Ccz1- and Mon1A/B -deficient cells. ( A ) Wild-type, NPC1-, C18orf8-, Ccz1 and Mon1A/B-deficient cells were treated for 24 hours with the NPC1 inhibitor U18666A to increase lysosomal cholesterol (pulse, top panels), followed by a 24 hours chase in the presence of LPDS and mevastatin (lower panels). Lysosomal cholesterol accumulation was visualised using Filipin co-staining with the LE/Ly marker CD63. ( B ) Colocalisation of Filipin with CD63 was plotted as Pearson correlation, calculated from 3 independent experiments with 6 representative fields per experiment and > 8 cells per field. ** p
Figure Legend Snippet: Rab7 activation by the MCC GEF controls NPC1-dependent lysosomal cholesterol export. ( A, B ) Lysosomal cholesterol export is abolished in NPC1 -, C18orf8 -, Ccz1- and Mon1A/B -deficient cells. ( A ) Wild-type, NPC1-, C18orf8-, Ccz1 and Mon1A/B-deficient cells were treated for 24 hours with the NPC1 inhibitor U18666A to increase lysosomal cholesterol (pulse, top panels), followed by a 24 hours chase in the presence of LPDS and mevastatin (lower panels). Lysosomal cholesterol accumulation was visualised using Filipin co-staining with the LE/Ly marker CD63. ( B ) Colocalisation of Filipin with CD63 was plotted as Pearson correlation, calculated from 3 independent experiments with 6 representative fields per experiment and > 8 cells per field. ** p

Techniques Used: Activation Assay, Staining, Marker

MCC -deficient cells lack an activation-dependent interaction between Rab7 and NPC1 and accumulate lysosomal cholesterol. ( A – C ) MCC -deficient cells accumulate lysosomal cholesterol and phenocopy NPC1 -deficiency. ( A ) Filipin staining of wild-type, C18orf8 -deficient and complemented C18orf8 -deficient cells; or ( C ) wild-type, Ccz1- and Mon1A/B -deficient cells. ( B ) Filipin co-staining with the LE/Ly markers Rab7 and LAMP1 in C18orf8 -deficient cells. ( D ) Theonellamides (TNM) immuno-gold labelling of C18orf8 -deficient cells, visualised by EM. ( E ) Filipin staining of wild-type, C18orf8 - and NPC1 -deficient cells. Scale bars = 10µm. ( F , G ) Rab7 interacts with the NPC1 cholesterol transporter in activation-dependent manner. ( F ) Immune-precipitation of HA-tagged NPC1 and detection of NPC1-interacting proteins using mass spectrometry. Interaction partners detected with > 2 peptides are indicated by abundance. ( G ) Immune precipitations of HA-tagged wild-type, dominant-negative (T22N) or constitutively active Rab7 (Q67L) reveal an activation-dependent interaction between Rab7 and endogenous NPC1. ( H ) The Rab7-NPC1 interaction is lost in MCC-deficient cells that lack Rab7 activation. Wild-type, C18orf8-, Ccz1 and Mon1A/B-deficient cells were stably transduced with the inactive NPC1-P692S-HA. HA-tagged NPC1 was immune precipitated and immune blotted for endogenous Rab7. The inactive NPC1-P692S was used to prevent altering lysosomal cholesterol content. ( I , J ) The Rab7-NPC1 interaction is independent of NPC1 activity or lysosomal cholesterol levels. ( I ) NPC1-deficient cells were complemented with HA-tagged wild-type or inactive P692S-mutant NPC1 and NPC1-HA immune-precipitations were analysed by immune blotting for endogenous Rab7. ( J ) Wild-type NPC1-HA complemented cells were treated with LPDS to decrease, or U18666A to increase lysosomal cholesterol levels and the NPC1-Rab7 interaction was probed using immune precipitation.
Figure Legend Snippet: MCC -deficient cells lack an activation-dependent interaction between Rab7 and NPC1 and accumulate lysosomal cholesterol. ( A – C ) MCC -deficient cells accumulate lysosomal cholesterol and phenocopy NPC1 -deficiency. ( A ) Filipin staining of wild-type, C18orf8 -deficient and complemented C18orf8 -deficient cells; or ( C ) wild-type, Ccz1- and Mon1A/B -deficient cells. ( B ) Filipin co-staining with the LE/Ly markers Rab7 and LAMP1 in C18orf8 -deficient cells. ( D ) Theonellamides (TNM) immuno-gold labelling of C18orf8 -deficient cells, visualised by EM. ( E ) Filipin staining of wild-type, C18orf8 - and NPC1 -deficient cells. Scale bars = 10µm. ( F , G ) Rab7 interacts with the NPC1 cholesterol transporter in activation-dependent manner. ( F ) Immune-precipitation of HA-tagged NPC1 and detection of NPC1-interacting proteins using mass spectrometry. Interaction partners detected with > 2 peptides are indicated by abundance. ( G ) Immune precipitations of HA-tagged wild-type, dominant-negative (T22N) or constitutively active Rab7 (Q67L) reveal an activation-dependent interaction between Rab7 and endogenous NPC1. ( H ) The Rab7-NPC1 interaction is lost in MCC-deficient cells that lack Rab7 activation. Wild-type, C18orf8-, Ccz1 and Mon1A/B-deficient cells were stably transduced with the inactive NPC1-P692S-HA. HA-tagged NPC1 was immune precipitated and immune blotted for endogenous Rab7. The inactive NPC1-P692S was used to prevent altering lysosomal cholesterol content. ( I , J ) The Rab7-NPC1 interaction is independent of NPC1 activity or lysosomal cholesterol levels. ( I ) NPC1-deficient cells were complemented with HA-tagged wild-type or inactive P692S-mutant NPC1 and NPC1-HA immune-precipitations were analysed by immune blotting for endogenous Rab7. ( J ) Wild-type NPC1-HA complemented cells were treated with LPDS to decrease, or U18666A to increase lysosomal cholesterol levels and the NPC1-Rab7 interaction was probed using immune precipitation.

Techniques Used: Activation Assay, Staining, Mass Spectrometry, Dominant Negative Mutation, Stable Transfection, Transduction, Activity Assay, Mutagenesis

Related Articles

Detection Assay:

Article Title: Novel mechanism of U18666A-induced tumour necrosis factor-α production in RAW 264·7 macrophage cells
Article Snippet: .. On the following day U18666A (1 µg/ml) or vehicle control was added into the cultures and incubated for a further 24 h. The slides were washed with phosphate-buffered saline five times and used for the filipin staining with the cholesterol cell-based detection assay kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer's instructions. .. Digital images were acquired immediately after filipin labelling under fluorescence microscopy.

Article Title: Protein Modifications Regulate the Role of 14-3-3γ Adaptor Protein in cAMP-induced Steroidogenesis in MA-10 Leydig Cells *
Article Snippet: .. The next day, the medium was replaced with medium without serum and with or without 250 n m TAT-14-3-3 Lys49 or TAT-14-3-3 Ser58 for 90 min followed by 1 m m 8-Br-cAMP treatment for 120 min. After the treatment period, medium was removed, cells were washed with 1× PBS, and cholesterol staining was performed using the Cholesterol Cell-Based Detection Assay kit, which applies filipin staining (Cayman Chemicals). ..

Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport
Article Snippet: .. Cholesterol localisation assay Localisation of intracellular cholesterol was detected through Filipin-III staining of cells using the Cayman’s Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). .. Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA).

Labeling:

Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung
Article Snippet: .. For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI). .. Cells were visualized using a Zeiss LSM 510 Meta microscope coupled to a Chameleon Ultra mode-locked femtosecond pulse laser (set at 720 nm for 2-photon excitation of filipin) and He-Ne and Ar lasers.

Concentration Assay:

Article Title: SCP2-mediated cholesterol membrane trafficking promotes the growth of pituitary adenomas via Hedgehog signaling activation
Article Snippet: .. Filipin staining and immunofluorescence Filipin III (Cayman, 70440) was dissolved in DMSO to a final concentration of 5 mg/ml. .. Cultured cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with 50 μg/ml filipin III for 2 h at 4 °C in the dark.

Incubation:

Article Title: Novel mechanism of U18666A-induced tumour necrosis factor-α production in RAW 264·7 macrophage cells
Article Snippet: .. On the following day U18666A (1 µg/ml) or vehicle control was added into the cultures and incubated for a further 24 h. The slides were washed with phosphate-buffered saline five times and used for the filipin staining with the cholesterol cell-based detection assay kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer's instructions. .. Digital images were acquired immediately after filipin labelling under fluorescence microscopy.

Article Title: A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export
Article Snippet: .. For Filipin staining, cells were fixed and incubated 1 hour with 0.05mg/ml Filipin III (Cayman) in 0.5% BSA. ..

Staining:

Article Title: Trypanosoma cruzi infection results in an increase in intracellular cholesterol
Article Snippet: .. Filipin staining was carried out on the frozen tissues embedded in OCT for cholesterol detection using 50ug/ml Filipin (Cayman Chemical, MI, USA) and following standard protocol [ ]. .. The NIH-ImageJ program was used to quantify fluorescence intensities for a minimum of five images (images taken using a 20 x objective to analyze approximately a total of 100 cells) in each experiment.

Article Title: Novel mechanism of U18666A-induced tumour necrosis factor-α production in RAW 264·7 macrophage cells
Article Snippet: .. On the following day U18666A (1 µg/ml) or vehicle control was added into the cultures and incubated for a further 24 h. The slides were washed with phosphate-buffered saline five times and used for the filipin staining with the cholesterol cell-based detection assay kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer's instructions. .. Digital images were acquired immediately after filipin labelling under fluorescence microscopy.

Article Title: A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export
Article Snippet: .. For Filipin staining, cells were fixed and incubated 1 hour with 0.05mg/ml Filipin III (Cayman) in 0.5% BSA. ..

Article Title: SCP2-mediated cholesterol membrane trafficking promotes the growth of pituitary adenomas via Hedgehog signaling activation
Article Snippet: .. Filipin staining and immunofluorescence Filipin III (Cayman, 70440) was dissolved in DMSO to a final concentration of 5 mg/ml. .. Cultured cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with 50 μg/ml filipin III for 2 h at 4 °C in the dark.

Article Title: Protein Modifications Regulate the Role of 14-3-3γ Adaptor Protein in cAMP-induced Steroidogenesis in MA-10 Leydig Cells *
Article Snippet: .. The next day, the medium was replaced with medium without serum and with or without 250 n m TAT-14-3-3 Lys49 or TAT-14-3-3 Ser58 for 90 min followed by 1 m m 8-Br-cAMP treatment for 120 min. After the treatment period, medium was removed, cells were washed with 1× PBS, and cholesterol staining was performed using the Cholesterol Cell-Based Detection Assay kit, which applies filipin staining (Cayman Chemicals). ..

Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung
Article Snippet: .. For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI). .. Cells were visualized using a Zeiss LSM 510 Meta microscope coupled to a Chameleon Ultra mode-locked femtosecond pulse laser (set at 720 nm for 2-photon excitation of filipin) and He-Ne and Ar lasers.

Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport
Article Snippet: .. Cholesterol localisation assay Localisation of intracellular cholesterol was detected through Filipin-III staining of cells using the Cayman’s Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). .. Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA).

Article Title: Protein Modifications Regulate the Role of 14-3-3γ Adaptor Protein in cAMP-induced Steroidogenesis in MA-10 Leydig Cells *
Article Snippet: .. Therefore, changes in lipid droplets (sites of storage of cholesterol and cholesteryl esters) and free cholesterol levels were evaluated in the presence of TAT-14-3-3γ fusion peptides using oil red O and filipin staining, respectively. ..

Immunofluorescence:

Article Title: SCP2-mediated cholesterol membrane trafficking promotes the growth of pituitary adenomas via Hedgehog signaling activation
Article Snippet: .. Filipin staining and immunofluorescence Filipin III (Cayman, 70440) was dissolved in DMSO to a final concentration of 5 mg/ml. .. Cultured cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with 50 μg/ml filipin III for 2 h at 4 °C in the dark.

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    Cayman Chemical filipin quantitative staining
    Syn acts on the content of plasma membrane cholesterol that affects calcium channel activation and neurotransmitter release. A , Bar chart showing cholesterol levels determined by quantitative fluorescent <t>filipin</t> staining. Cortical neurons incubated with
    Filipin Quantitative Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin quantitative staining/product/Cayman Chemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin quantitative staining - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    91
    Cayman Chemical o methyl serine dodecylamide hydrochloride msdh filipin staining
    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - <t>Filipin</t> staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent <t>MSDH.</t> Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.
    O Methyl Serine Dodecylamide Hydrochloride Msdh Filipin Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o methyl serine dodecylamide hydrochloride msdh filipin staining/product/Cayman Chemical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o methyl serine dodecylamide hydrochloride msdh filipin staining - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    93
    Cayman Chemical filipin staining
    Cholesterol accumulation in type II cells visualized with <t>filipin</t> after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P
    Filipin Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin staining/product/Cayman Chemical
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    filipin staining - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Syn acts on the content of plasma membrane cholesterol that affects calcium channel activation and neurotransmitter release. A , Bar chart showing cholesterol levels determined by quantitative fluorescent filipin staining. Cortical neurons incubated with

    Journal: The Journal of Neuroscience

    Article Title: Exogenous α-Synuclein Decreases Raft Partitioning of Cav2.2 Channels Inducing Dopamine Release

    doi: 10.1523/JNEUROSCI.0608-14.2014

    Figure Lengend Snippet: Syn acts on the content of plasma membrane cholesterol that affects calcium channel activation and neurotransmitter release. A , Bar chart showing cholesterol levels determined by quantitative fluorescent filipin staining. Cortical neurons incubated with

    Article Snippet: Filipin quantitative staining of fixed cells was performed accordingly to a modification of a commercially available kit (catalog #10009779; Cayman Chemicals).

    Techniques: Activation Assay, Staining, Incubation

    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.

    Journal: Scientific Reports

    Article Title: 2-Hydroxypropyl-beta-cyclodextrin (HPβCD) reduces age-related lipofuscin accumulation through a cholesterol-associated pathway

    doi: 10.1038/s41598-017-02387-8

    Figure Lengend Snippet: Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.

    Article Snippet: Cholesterol detection with filipin and O-methyl-serine dodecylamide hydrochloride (MSDH) Filipin staining was performed using the Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical 10009779) and protocol.

    Techniques: Staining

    Cholesterol accumulation in type II cells visualized with filipin after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung

    doi: 10.1152/ajplung.00383.2011

    Figure Lengend Snippet: Cholesterol accumulation in type II cells visualized with filipin after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P

    Article Snippet: For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI).

    Techniques: Labeling, Quantitation Assay, Staining

    Lamellar bodies were enriched with cholesterol after treatment with UA. Rat type II cells incubated with 10% FCS were untreated (Control) or treated with UA for 18 h. Cells were fixed and labeled with ABCA3 (gray, 1 and 5 ) and filipin (gray, 2 and 6 ). Merged image ( 3 and 7 ) of ABCA3 (green) and filipin (blue) and a merge with light ( 4 and 8 ) are shown. Outlines of cells are marked by white dashed lines. Two lamellar bodies ( 1′–4′ and 5′–8′ ) are highlighted for enlargement. ABCA3 ( 1′ and 5′ ), filipin ( 2′ and 6′ ), merged image ( 3′ and 7′ ), and merge with light ( 4′ and 8′ ) of enlarged lamellar bodies are shown. Scale bars, 5 μm.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung

    doi: 10.1152/ajplung.00383.2011

    Figure Lengend Snippet: Lamellar bodies were enriched with cholesterol after treatment with UA. Rat type II cells incubated with 10% FCS were untreated (Control) or treated with UA for 18 h. Cells were fixed and labeled with ABCA3 (gray, 1 and 5 ) and filipin (gray, 2 and 6 ). Merged image ( 3 and 7 ) of ABCA3 (green) and filipin (blue) and a merge with light ( 4 and 8 ) are shown. Outlines of cells are marked by white dashed lines. Two lamellar bodies ( 1′–4′ and 5′–8′ ) are highlighted for enlargement. ABCA3 ( 1′ and 5′ ), filipin ( 2′ and 6′ ), merged image ( 3′ and 7′ ), and merge with light ( 4′ and 8′ ) of enlarged lamellar bodies are shown. Scale bars, 5 μm.

    Article Snippet: For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI).

    Techniques: Incubation, Labeling

    Cholesterol accumulation in NPC1- and NPC2-positive lamellar bodies after treatment with compound UA. A : rat type II cells were treated with UA and triple-labeled with ABCA3 (green), filipin (gray or blue), and NPC1 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC1-positive/filipin-high lamellar body is shown in box 1 , and an NPC-1-positive/filipin-low lamellar body is shown in box 2 . B : type II cells treated with UA and triple-labeled with ABCA3 (green), filipin (gray), and NPC2 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC2-positive/filipin-high lamellar body is shown in box 1 , and a NPC-2-positive/filipin-low lamellar body is shown in box 2 . Scale bars, 5 μm.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung

    doi: 10.1152/ajplung.00383.2011

    Figure Lengend Snippet: Cholesterol accumulation in NPC1- and NPC2-positive lamellar bodies after treatment with compound UA. A : rat type II cells were treated with UA and triple-labeled with ABCA3 (green), filipin (gray or blue), and NPC1 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC1-positive/filipin-high lamellar body is shown in box 1 , and an NPC-1-positive/filipin-low lamellar body is shown in box 2 . B : type II cells treated with UA and triple-labeled with ABCA3 (green), filipin (gray), and NPC2 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC2-positive/filipin-high lamellar body is shown in box 1 , and a NPC-2-positive/filipin-low lamellar body is shown in box 2 . Scale bars, 5 μm.

    Article Snippet: For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI).

    Techniques: Labeling