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Genmed filipin staining kit
Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using <t>Filipin</t> staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P
Filipin Staining Kit, supplied by Genmed, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/filipin staining kit/product/Genmed
Average 93 stars, based on 4 article reviews
Price from $9.99 to $1999.99
filipin staining kit - by Bioz Stars, 2020-04
93/100 stars

Images

1) Product Images from "Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages"

Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2018.9048

Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P
Figure Legend Snippet: Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P

Techniques Used: Staining, Standard Deviation

2) Product Images from "Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages"

Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2018.9048

Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P
Figure Legend Snippet: Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P

Techniques Used: Staining, Standard Deviation

Related Articles

Immunohistochemistry:

Article Title: Rutaecarpine suppresses atherosclerosis in ApoE−/− mice through upregulating ABCA1 and SR-BI within RCT [S]
Article Snippet: Paragraph title: Histology and immunohistochemical staining ... Mouse macrophage-specific antibody (Mac-3) immunochemistry staining and Filipin staining were performed on the cryosections (7 μm) from aortic sinus using Mac-3 monoclonal antibody (BD PharmingenTM ) and a Filipin staining kit (Genmed Sci), respectively.

Fluorescence:

Article Title: Rutaecarpine suppresses atherosclerosis in ApoE−/− mice through upregulating ABCA1 and SR-BI within RCT [S]
Article Snippet: Mouse macrophage-specific antibody (Mac-3) immunochemistry staining and Filipin staining were performed on the cryosections (7 μm) from aortic sinus using Mac-3 monoclonal antibody (BD PharmingenTM ) and a Filipin staining kit (Genmed Sci), respectively. .. The Filipin staining images were viewed under a fluorescence microscope (excitation 340 nm, emission 430 nm) at 200× magnification.

Multiple Displacement Amplification:

Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages
Article Snippet: SOD and MDA were measured using assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). .. A Filipin staining kit was purchased from Shanghai Genmed (Shanghai, China), and DAPI staining solution was purchased from Shanghai Beyotime.

Microscopy:

Article Title: Rutaecarpine suppresses atherosclerosis in ApoE−/− mice through upregulating ABCA1 and SR-BI within RCT [S]
Article Snippet: All sections were examined under a Nikon E600 microscope. .. Mouse macrophage-specific antibody (Mac-3) immunochemistry staining and Filipin staining were performed on the cryosections (7 μm) from aortic sinus using Mac-3 monoclonal antibody (BD PharmingenTM ) and a Filipin staining kit (Genmed Sci), respectively.

Light Microscopy:

Article Title: Rutaecarpine suppresses atherosclerosis in ApoE−/− mice through upregulating ABCA1 and SR-BI within RCT [S]
Article Snippet: Mouse macrophage-specific antibody (Mac-3) immunochemistry staining and Filipin staining were performed on the cryosections (7 μm) from aortic sinus using Mac-3 monoclonal antibody (BD PharmingenTM ) and a Filipin staining kit (Genmed Sci), respectively. .. Mac-3 staining slides were examined with a light microscope at 200× magnification ( ).

BIA-KA:

Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages
Article Snippet: An enhanced BCA protein assay kit was used as was an SDS-PAGE preparation kit (both from Shanghai Beyotime). .. A Filipin staining kit was purchased from Shanghai Genmed (Shanghai, China), and DAPI staining solution was purchased from Shanghai Beyotime.

Article Title: Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1
Article Snippet: RIPA lysis buffer (P0013B), PMSF (ST505), the BCA protein assay kit (P0010), SDS-PAGE protein loading buffer (5X) (P0015L), SDS-PAGE gel preparation kit (P0012A), Protein ladder (P0071) and BeyoECL Plus (P0018S) were obtained from Shanghai Beyotime Biotechnology Co., Ltd. EZ-Buffers H 10X TBST buffer (C520009) was supplied by Shanghai Sangon Biotechnology Co., Ltd. Mayer hematoxylin staining reagent (1092490500) was supplied by Merck KGaA. .. The Filipin Staining kit (GMS80059.3) was obtained from Shanghai Genmed Gene Medicine Technology Co., Ltd.

Lysis:

Article Title: Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1
Article Snippet: RIPA lysis buffer (P0013B), PMSF (ST505), the BCA protein assay kit (P0010), SDS-PAGE protein loading buffer (5X) (P0015L), SDS-PAGE gel preparation kit (P0012A), Protein ladder (P0071) and BeyoECL Plus (P0018S) were obtained from Shanghai Beyotime Biotechnology Co., Ltd. EZ-Buffers H 10X TBST buffer (C520009) was supplied by Shanghai Sangon Biotechnology Co., Ltd. Mayer hematoxylin staining reagent (1092490500) was supplied by Merck KGaA. .. The Filipin Staining kit (GMS80059.3) was obtained from Shanghai Genmed Gene Medicine Technology Co., Ltd.

SDS Page:

Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages
Article Snippet: An enhanced BCA protein assay kit was used as was an SDS-PAGE preparation kit (both from Shanghai Beyotime). .. A Filipin staining kit was purchased from Shanghai Genmed (Shanghai, China), and DAPI staining solution was purchased from Shanghai Beyotime.

Article Title: Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1
Article Snippet: RIPA lysis buffer (P0013B), PMSF (ST505), the BCA protein assay kit (P0010), SDS-PAGE protein loading buffer (5X) (P0015L), SDS-PAGE gel preparation kit (P0012A), Protein ladder (P0071) and BeyoECL Plus (P0018S) were obtained from Shanghai Beyotime Biotechnology Co., Ltd. EZ-Buffers H 10X TBST buffer (C520009) was supplied by Shanghai Sangon Biotechnology Co., Ltd. Mayer hematoxylin staining reagent (1092490500) was supplied by Merck KGaA. .. The Filipin Staining kit (GMS80059.3) was obtained from Shanghai Genmed Gene Medicine Technology Co., Ltd.

Staining:

Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages
Article Snippet: .. A Filipin staining kit was purchased from Shanghai Genmed (Shanghai, China), and DAPI staining solution was purchased from Shanghai Beyotime. .. Macrophage cell culture RAW264.7 cells were cultured in DMEM medium containing 10% FBS in a humidified atmosphere at 37°C, 5% CO2 .

Article Title: Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1
Article Snippet: .. The Filipin Staining kit (GMS80059.3) was obtained from Shanghai Genmed Gene Medicine Technology Co., Ltd. .. Modeling and grouping A total of 54 12-week-old apoE−/− mice were divided into 3 groups using a random number table method ( ) after being fed a regular diet for 1 week.

Article Title: Rutaecarpine suppresses atherosclerosis in ApoE−/− mice through upregulating ABCA1 and SR-BI within RCT [S]
Article Snippet: .. Mouse macrophage-specific antibody (Mac-3) immunochemistry staining and Filipin staining were performed on the cryosections (7 μm) from aortic sinus using Mac-3 monoclonal antibody (BD PharmingenTM ) and a Filipin staining kit (Genmed Sci), respectively. .. Mac-3 staining slides were examined with a light microscope at 200× magnification ( ).

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  • 93
    Genmed filipin staining kit
    Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using <t>Filipin</t> staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P
    Filipin Staining Kit, supplied by Genmed, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin staining kit/product/Genmed
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    filipin staining kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    91
    Genmed filipin fluorescence staining kit
    Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, <t>Filipin</t> staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p
    Filipin Fluorescence Staining Kit, supplied by Genmed, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin fluorescence staining kit/product/Genmed
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin fluorescence staining kit - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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    Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P

    Journal: Molecular Medicine Reports

    Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages

    doi: 10.3892/mmr.2018.9048

    Figure Lengend Snippet: Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P

    Article Snippet: A Filipin staining kit was purchased from Shanghai Genmed (Shanghai, China), and DAPI staining solution was purchased from Shanghai Beyotime.

    Techniques: Staining, Standard Deviation

    Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P

    Journal: Molecular Medicine Reports

    Article Title: Quercetin protects against ox-LDL-induced injury via regulation of ABCAl, LXR-α and PCSK9 in RAW264.7 macrophages

    doi: 10.3892/mmr.2018.9048

    Figure Lengend Snippet: Quercetin and lipid accumulation in RAW264.7 cells induced by ox-LDL. (A) RAW264.7 cells were treated with quercetin and ox-LDL and the lipid accumulation was measured by oil red staining. (B) RAW264.7 cells were treated with quercetin and ox-LDL and the intracellular free cholesterol was measured using Filipin staining. (C) RAW264.7 cells were treated with quercetin and ox-LDL and the total cellular cholesterol was measured. Data are presented as the mean ± standard deviation. *P

    Article Snippet: A Filipin staining kit was purchased from Shanghai Genmed (Shanghai, China), and DAPI staining solution was purchased from Shanghai Beyotime.

    Techniques: Staining, Standard Deviation

    Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, Filipin staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p

    Journal: Scientific Reports

    Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism

    doi: 10.1038/srep31381

    Figure Lengend Snippet: Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, Filipin staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p

    Article Snippet: Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol.

    Techniques: Mouse Assay, Cholesterol Assay, Staining, Real-time Polymerase Chain Reaction

    Treatment with 4-PBA alleviated lipid metabolic disorders in SCH mice. (A) Levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the serum of all mice were determined after 4-PBA was administered for 4 weeks (n = 6–12). (B) Hepatic TC contents were detected using a cholesterol assay kit after 4-PBA was administered for 4 weeks (n = 6–12). (C) Hepatic TG contents were detected using a triglyceride assay kit after 4-PBA was administered for 4 weeks (n = 6–13). (D) Filipin staining was used to measure hepatic cholesterol deposition after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (E) Oil red O staining was used to measure hepatic TG accumulation after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (F) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 18th week (n = 4–6). The data are presented as the mean ± SD. *p

    Journal: Scientific Reports

    Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism

    doi: 10.1038/srep31381

    Figure Lengend Snippet: Treatment with 4-PBA alleviated lipid metabolic disorders in SCH mice. (A) Levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the serum of all mice were determined after 4-PBA was administered for 4 weeks (n = 6–12). (B) Hepatic TC contents were detected using a cholesterol assay kit after 4-PBA was administered for 4 weeks (n = 6–12). (C) Hepatic TG contents were detected using a triglyceride assay kit after 4-PBA was administered for 4 weeks (n = 6–13). (D) Filipin staining was used to measure hepatic cholesterol deposition after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (E) Oil red O staining was used to measure hepatic TG accumulation after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (F) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 18th week (n = 4–6). The data are presented as the mean ± SD. *p

    Article Snippet: Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol.

    Techniques: Mouse Assay, Cholesterol Assay, Staining, Real-time Polymerase Chain Reaction