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Cayman Chemical filipin stain
Filipin Stain, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/filipin stain/product/Cayman Chemical
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
filipin stain - by Bioz Stars, 2020-07
88/100 stars

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Modification:

Article Title: Impaired LRP6-TCF7L2 activity enhances smooth muscle cell plasticity and causes coronary artery disease
Article Snippet: .. Wnt3a from R & D Systems; Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin streptomycin cocktail, Trypsin-EDTA solution, and TRIzol were purchased from GIBCO/Invitrogen; polyvinylidinefluoride membranes from Bio-Rad Laboratories, Filipin stain from Cayman Chemical. .. Antibodies for PDGFRα, β Catenin, TCF7L2, SP1, CD3 and protein A/G agarose gel were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).

Staining:

Article Title: Impaired LRP6-TCF7L2 activity enhances smooth muscle cell plasticity and causes coronary artery disease
Article Snippet: .. Wnt3a from R & D Systems; Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin streptomycin cocktail, Trypsin-EDTA solution, and TRIzol were purchased from GIBCO/Invitrogen; polyvinylidinefluoride membranes from Bio-Rad Laboratories, Filipin stain from Cayman Chemical. .. Antibodies for PDGFRα, β Catenin, TCF7L2, SP1, CD3 and protein A/G agarose gel were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).

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    Cayman Chemical filipin quantitative staining
    Syn acts on the content of plasma membrane cholesterol that affects calcium channel activation and neurotransmitter release. A , Bar chart showing cholesterol levels determined by quantitative fluorescent <t>filipin</t> staining. Cortical neurons incubated with
    Filipin Quantitative Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin quantitative staining/product/Cayman Chemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin quantitative staining - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    91
    Cayman Chemical o methyl serine dodecylamide hydrochloride msdh filipin staining
    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - <t>Filipin</t> staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent <t>MSDH.</t> Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.
    O Methyl Serine Dodecylamide Hydrochloride Msdh Filipin Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o methyl serine dodecylamide hydrochloride msdh filipin staining/product/Cayman Chemical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o methyl serine dodecylamide hydrochloride msdh filipin staining - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    93
    Cayman Chemical filipin staining
    Cholesterol accumulation in type II cells visualized with <t>filipin</t> after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P
    Filipin Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin staining/product/Cayman Chemical
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    filipin staining - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Syn acts on the content of plasma membrane cholesterol that affects calcium channel activation and neurotransmitter release. A , Bar chart showing cholesterol levels determined by quantitative fluorescent filipin staining. Cortical neurons incubated with

    Journal: The Journal of Neuroscience

    Article Title: Exogenous α-Synuclein Decreases Raft Partitioning of Cav2.2 Channels Inducing Dopamine Release

    doi: 10.1523/JNEUROSCI.0608-14.2014

    Figure Lengend Snippet: Syn acts on the content of plasma membrane cholesterol that affects calcium channel activation and neurotransmitter release. A , Bar chart showing cholesterol levels determined by quantitative fluorescent filipin staining. Cortical neurons incubated with

    Article Snippet: Filipin quantitative staining of fixed cells was performed accordingly to a modification of a commercially available kit (catalog #10009779; Cayman Chemicals).

    Techniques: Activation Assay, Staining, Incubation

    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.

    Journal: Scientific Reports

    Article Title: 2-Hydroxypropyl-beta-cyclodextrin (HPβCD) reduces age-related lipofuscin accumulation through a cholesterol-associated pathway

    doi: 10.1038/s41598-017-02387-8

    Figure Lengend Snippet: Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.

    Article Snippet: Cholesterol detection with filipin and O-methyl-serine dodecylamide hydrochloride (MSDH) Filipin staining was performed using the Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical 10009779) and protocol.

    Techniques: Staining

    Cholesterol accumulation in type II cells visualized with filipin after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung

    doi: 10.1152/ajplung.00383.2011

    Figure Lengend Snippet: Cholesterol accumulation in type II cells visualized with filipin after treatment with UA. A : type II cells untreated (Control) or treated with 0.5 μg/ml UA for 18 h in 10% FCS were fixed and labeled with filipin. Filipin alone (Filipin, gray) and the corresponding light micrograph (Light) are shown. Outline of cells in all images is marked by dashed lines. A single UA-treated cell (box) is selected for enlargement. Enlarged panels [UA (enlarged)] for filipin (gray) and light micrographs (Light) are shown. Arrows indicate a lamellar body-like structure that is positive for filipin; arrowheads indicate a lamellar body-like structure that has relatively little filipin label. Scale bars, 20 μm. B : quantitation of filipin staining in type II cells. Pixel intensity was quantified in ImageJ as arbitrary units for whole cells and late endosome/lysosome-like storage organelle (LSO). In each experiment, 3–8 fields per treatment were quantitated. Top : whole cell. Values are means ± SE ( n = 5 fields from a representative experiment of the 4 performed). UA stimulation of filipin staining was statistically significant from control (Ctrl) for each experiment and ranged from 2.2- to 8.1-fold over control for the 4 experiments. * P

    Article Snippet: For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI).

    Techniques: Labeling, Quantitation Assay, Staining

    Lamellar bodies were enriched with cholesterol after treatment with UA. Rat type II cells incubated with 10% FCS were untreated (Control) or treated with UA for 18 h. Cells were fixed and labeled with ABCA3 (gray, 1 and 5 ) and filipin (gray, 2 and 6 ). Merged image ( 3 and 7 ) of ABCA3 (green) and filipin (blue) and a merge with light ( 4 and 8 ) are shown. Outlines of cells are marked by white dashed lines. Two lamellar bodies ( 1′–4′ and 5′–8′ ) are highlighted for enlargement. ABCA3 ( 1′ and 5′ ), filipin ( 2′ and 6′ ), merged image ( 3′ and 7′ ), and merge with light ( 4′ and 8′ ) of enlarged lamellar bodies are shown. Scale bars, 5 μm.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung

    doi: 10.1152/ajplung.00383.2011

    Figure Lengend Snippet: Lamellar bodies were enriched with cholesterol after treatment with UA. Rat type II cells incubated with 10% FCS were untreated (Control) or treated with UA for 18 h. Cells were fixed and labeled with ABCA3 (gray, 1 and 5 ) and filipin (gray, 2 and 6 ). Merged image ( 3 and 7 ) of ABCA3 (green) and filipin (blue) and a merge with light ( 4 and 8 ) are shown. Outlines of cells are marked by white dashed lines. Two lamellar bodies ( 1′–4′ and 5′–8′ ) are highlighted for enlargement. ABCA3 ( 1′ and 5′ ), filipin ( 2′ and 6′ ), merged image ( 3′ and 7′ ), and merge with light ( 4′ and 8′ ) of enlarged lamellar bodies are shown. Scale bars, 5 μm.

    Article Snippet: For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI).

    Techniques: Incubation, Labeling

    Cholesterol accumulation in NPC1- and NPC2-positive lamellar bodies after treatment with compound UA. A : rat type II cells were treated with UA and triple-labeled with ABCA3 (green), filipin (gray or blue), and NPC1 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC1-positive/filipin-high lamellar body is shown in box 1 , and an NPC-1-positive/filipin-low lamellar body is shown in box 2 . B : type II cells treated with UA and triple-labeled with ABCA3 (green), filipin (gray), and NPC2 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC2-positive/filipin-high lamellar body is shown in box 1 , and a NPC-2-positive/filipin-low lamellar body is shown in box 2 . Scale bars, 5 μm.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung

    doi: 10.1152/ajplung.00383.2011

    Figure Lengend Snippet: Cholesterol accumulation in NPC1- and NPC2-positive lamellar bodies after treatment with compound UA. A : rat type II cells were treated with UA and triple-labeled with ABCA3 (green), filipin (gray or blue), and NPC1 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC1-positive/filipin-high lamellar body is shown in box 1 , and an NPC-1-positive/filipin-low lamellar body is shown in box 2 . B : type II cells treated with UA and triple-labeled with ABCA3 (green), filipin (gray), and NPC2 (red). In merged (Merge) micrograph, filipin is shown in blue. An enlarged image of an NPC2-positive/filipin-high lamellar body is shown in box 1 , and a NPC-2-positive/filipin-low lamellar body is shown in box 2 . Scale bars, 5 μm.

    Article Snippet: For filipin staining, cells were labeled according to the manufacturer's instructions (Filipin III, Cayman Chemical, Ann Arbor, MI).

    Techniques: Labeling