filipin pbs  (Millipore)


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    Name:
    Dimethyl
    Description:

    Catalog Number:
    901460
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    Structured Review

    Millipore filipin pbs
    Dimethyl

    https://www.bioz.com/result/filipin pbs/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin pbs - by Bioz Stars, 2020-07
    99/100 stars

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    hptlc plates

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    Related Articles

    Electron Paramagnetic Resonance:

    Article Title: A Different Pattern of Production and Scavenging of Reactive Oxygen Species in Halophytic Eutrema salsugineum (Thellungiella salsuginea) Plants in Comparison to Arabidopsis thaliana and Its Relation to Salt Stress Signaling
    Article Snippet: .. Electron Paramagnetic Resonance (EPR) Measurements Production of O 2 •– and H2 O2 by thylakoids from A. thaliana and E. salsugineum water-treated plants was detected by electron paramagnetic resonance (EPR) spin-trapping spectroscopy using DMPO (5,5-dimethyl-pyrroline N -oxide; Sigma-Aldrich, USA) and POBN [α-(4-pyridyl-1-oxide)-N -tertbutylnitrone; Sigma-Aldrich] as the spin trap, respectively, as described earlier ( ). .. Shortly, for O 2 •– detection, isolated thylakoids (chlorophyll concentration 200 μg mL-1 ) were mixed with DMPO to a final concentration 50 mM, transferred to a flat cell and illuminated for 5 min at 500 μmol quanta m-2 s-1 within the EPR spectrometer MiniScope MS300 (Magnettech GmbH, Germany).

    MTT Assay:

    Article Title: Fabrication and Characterization of Core-Shell Electrospun Fibrous Mats Containing Medicinal Herbs for Wound Healing and Skin Tissue Engineering
    Article Snippet: .. MTT Assay The dimethyl-thiazole diphenyl tetrazolium bromide (MTT, Sigma, USA) assay was employed as an indirect method to determine the cell viability. .. The specimens were first exposed to an ultraviolet irradiation for 45 min for sterilization.

    Article Title: Inhibition of Cell Survival by Curcumin Is Associated with Downregulation of Cell Division Cycle 20 (Cdc20) in Pancreatic Cancer Cells
    Article Snippet: .. The anti-β-actin, anti-Tubulin, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Inhibitor of APC/C (Apcin), a Cdc20 inhibitor, was purchased from BostonBiochem (Boston, MA, USA).

    Concentration Assay:

    Article Title: The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy
    Article Snippet: .. Chemicals Withaferin-A (WFA) (Tocris, Abingdon, UK) was diluted in dimethyl sulphoxide (DMSO) (Sigma-Aldrich, Irvine, UK) to a stock concentration of 1mg/ml. ..

    other:

    Article Title: Differential Stimulation of the Na+/H+ Exchanger Determines Chloroquine Uptake in Plasmodium falciparum
    Article Snippet: The NHE inhibitors examined were: amiloride ( Sigma Chemical Co. ); DMA (5-[N ,N -dimethyl]amiloride; Sigma Chemical Co. ); EIPA (5-[N -ethyl- N -isopropyl]amiloride; Hoechst A.G. Frankfurt, Germany); HMA (5-[N ,N -hexamethylene]amiloride; Molecular Probes, Inc.); Hoe 370 (5-chloro- 2-indoloyl guanidine; Hoechst); IBMA (5-[N -isobutyl-N -methyl]amiloride; Molecular Probes Inc.).

    Spectroscopy:

    Article Title: A Different Pattern of Production and Scavenging of Reactive Oxygen Species in Halophytic Eutrema salsugineum (Thellungiella salsuginea) Plants in Comparison to Arabidopsis thaliana and Its Relation to Salt Stress Signaling
    Article Snippet: .. Electron Paramagnetic Resonance (EPR) Measurements Production of O 2 •– and H2 O2 by thylakoids from A. thaliana and E. salsugineum water-treated plants was detected by electron paramagnetic resonance (EPR) spin-trapping spectroscopy using DMPO (5,5-dimethyl-pyrroline N -oxide; Sigma-Aldrich, USA) and POBN [α-(4-pyridyl-1-oxide)-N -tertbutylnitrone; Sigma-Aldrich] as the spin trap, respectively, as described earlier ( ). .. Shortly, for O 2 •– detection, isolated thylakoids (chlorophyll concentration 200 μg mL-1 ) were mixed with DMPO to a final concentration 50 mM, transferred to a flat cell and illuminated for 5 min at 500 μmol quanta m-2 s-1 within the EPR spectrometer MiniScope MS300 (Magnettech GmbH, Germany).

    Spin Trapping:

    Article Title: A Different Pattern of Production and Scavenging of Reactive Oxygen Species in Halophytic Eutrema salsugineum (Thellungiella salsuginea) Plants in Comparison to Arabidopsis thaliana and Its Relation to Salt Stress Signaling
    Article Snippet: .. Electron Paramagnetic Resonance (EPR) Measurements Production of O 2 •– and H2 O2 by thylakoids from A. thaliana and E. salsugineum water-treated plants was detected by electron paramagnetic resonance (EPR) spin-trapping spectroscopy using DMPO (5,5-dimethyl-pyrroline N -oxide; Sigma-Aldrich, USA) and POBN [α-(4-pyridyl-1-oxide)-N -tertbutylnitrone; Sigma-Aldrich] as the spin trap, respectively, as described earlier ( ). .. Shortly, for O 2 •– detection, isolated thylakoids (chlorophyll concentration 200 μg mL-1 ) were mixed with DMPO to a final concentration 50 mM, transferred to a flat cell and illuminated for 5 min at 500 μmol quanta m-2 s-1 within the EPR spectrometer MiniScope MS300 (Magnettech GmbH, Germany).

    Injection:

    Article Title: Involvement of Mitogen-Activated Protein Kinase Pathways in Expression of the Water Channel Protein Aquaporin-4 after Ischemia in Rat Cortical Astrocytes
    Article Snippet: .. Thirty minutes before the ischemic insult, a total of 10 μL of 100 μM of SB203580 (100 μM dissolved in 1% dimethyl sulfoxide), a specific p38 inhibitor (Calbiochem), or vehicle (1% dimethyl sulfoxide), were injected stereotaxically into the right lateral cerebroventricle (coordinates with reference to the bregma: 1.4 mm lateral, 0.8 mm posterior, 3.6 mm deep) using a 26-G Hamilton microsyringe (80330; Hamilton Company, Reno, NV). ..

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  • 99
    Millipore pp2
    Caveolin-1 and EGFR co-localization as a feature of non-canonical EGFR activation. Epithelial cells (RLE-6TN) were exposed (5 min) to carbon nanoparticles (CNP), non-nano carbon particles (CP), each 10 µg/cm 2 , (50 µM) H 2 O 2 , (5 µM) C6 ceramide, or EGF (100 ng/mL), respectively. ( A ) Subcellular localization of EGFR (red Alexa flour 594) and caveolin-1 (green Alexa flour 488). Co-localization is visualized by the yellow color in merged images; ( B ) Subcellular localization of EGFR and caveolin-1 in cells pre-treated with inhibitors of carbon nanoparticle-specific signaling prior to particle or EGF exposure: SFK inhibitor <t>PP2</t> (10 µM), 1 mM ectoine ( E ), 75 µM α-tocopherol (Toco), and 1 µg/mL filipin III (Fil). Co-localization is visualized by the yellow color in merged images; ( C ) Subcellular localization of caveolin-1 (red Alexa fluor 594) after exposure to carbon nanoparticles (CNP), carbon particles (CP), or hydrogen peroxide (H 2 O 2 ); ( D ) Quantification and representative Western blots of caveolin-1 in lipid raft fraction of RLE-6TN cells exposed to CNP (10 µg/cm 2 ). Raft and non-raft fraction were isolated from density gradients after ultracentrifugation. Pre-treatment of cells with α-tocopherol (Toco) was applied as an antioxidant strategy. GAPDH was used as a control protein not associated with lipid rafts. The bars in the graph represent the additive immune signals of raft and non-raft fractions, which was indicated in the representative original Western blots; ( E ) Quantification and representative Western-blot of EGFR phosphorylation at Tyr 845 . Nuclei were stained with DAPI (blue). Scale bars represent 20 µm; *, which was significantly different to PBS control ( p
    Pp2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp2/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    pp2 - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore microcapillary flow cytometer
    Caveolin-1 and EGFR co-localization as a feature of non-canonical EGFR activation. Epithelial cells (RLE-6TN) were exposed (5 min) to carbon nanoparticles (CNP), non-nano carbon particles (CP), each 10 µg/cm 2 , (50 µM) H 2 O 2 , (5 µM) C6 ceramide, or EGF (100 ng/mL), respectively. ( A ) Subcellular localization of EGFR (red Alexa flour 594) and caveolin-1 (green Alexa flour 488). Co-localization is visualized by the yellow color in merged images; ( B ) Subcellular localization of EGFR and caveolin-1 in cells pre-treated with inhibitors of carbon nanoparticle-specific signaling prior to particle or EGF exposure: SFK inhibitor <t>PP2</t> (10 µM), 1 mM ectoine ( E ), 75 µM α-tocopherol (Toco), and 1 µg/mL filipin III (Fil). Co-localization is visualized by the yellow color in merged images; ( C ) Subcellular localization of caveolin-1 (red Alexa fluor 594) after exposure to carbon nanoparticles (CNP), carbon particles (CP), or hydrogen peroxide (H 2 O 2 ); ( D ) Quantification and representative Western blots of caveolin-1 in lipid raft fraction of RLE-6TN cells exposed to CNP (10 µg/cm 2 ). Raft and non-raft fraction were isolated from density gradients after ultracentrifugation. Pre-treatment of cells with α-tocopherol (Toco) was applied as an antioxidant strategy. GAPDH was used as a control protein not associated with lipid rafts. The bars in the graph represent the additive immune signals of raft and non-raft fractions, which was indicated in the representative original Western blots; ( E ) Quantification and representative Western-blot of EGFR phosphorylation at Tyr 845 . Nuclei were stained with DAPI (blue). Scale bars represent 20 µm; *, which was significantly different to PBS control ( p
    Microcapillary Flow Cytometer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcapillary flow cytometer/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    microcapillary flow cytometer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Caveolin-1 and EGFR co-localization as a feature of non-canonical EGFR activation. Epithelial cells (RLE-6TN) were exposed (5 min) to carbon nanoparticles (CNP), non-nano carbon particles (CP), each 10 µg/cm 2 , (50 µM) H 2 O 2 , (5 µM) C6 ceramide, or EGF (100 ng/mL), respectively. ( A ) Subcellular localization of EGFR (red Alexa flour 594) and caveolin-1 (green Alexa flour 488). Co-localization is visualized by the yellow color in merged images; ( B ) Subcellular localization of EGFR and caveolin-1 in cells pre-treated with inhibitors of carbon nanoparticle-specific signaling prior to particle or EGF exposure: SFK inhibitor PP2 (10 µM), 1 mM ectoine ( E ), 75 µM α-tocopherol (Toco), and 1 µg/mL filipin III (Fil). Co-localization is visualized by the yellow color in merged images; ( C ) Subcellular localization of caveolin-1 (red Alexa fluor 594) after exposure to carbon nanoparticles (CNP), carbon particles (CP), or hydrogen peroxide (H 2 O 2 ); ( D ) Quantification and representative Western blots of caveolin-1 in lipid raft fraction of RLE-6TN cells exposed to CNP (10 µg/cm 2 ). Raft and non-raft fraction were isolated from density gradients after ultracentrifugation. Pre-treatment of cells with α-tocopherol (Toco) was applied as an antioxidant strategy. GAPDH was used as a control protein not associated with lipid rafts. The bars in the graph represent the additive immune signals of raft and non-raft fractions, which was indicated in the representative original Western blots; ( E ) Quantification and representative Western-blot of EGFR phosphorylation at Tyr 845 . Nuclei were stained with DAPI (blue). Scale bars represent 20 µm; *, which was significantly different to PBS control ( p

    Journal: Nanomaterials

    Article Title: Non-Canonical Activation of the Epidermal Growth Factor Receptor by Carbon Nanoparticles

    doi: 10.3390/nano8040267

    Figure Lengend Snippet: Caveolin-1 and EGFR co-localization as a feature of non-canonical EGFR activation. Epithelial cells (RLE-6TN) were exposed (5 min) to carbon nanoparticles (CNP), non-nano carbon particles (CP), each 10 µg/cm 2 , (50 µM) H 2 O 2 , (5 µM) C6 ceramide, or EGF (100 ng/mL), respectively. ( A ) Subcellular localization of EGFR (red Alexa flour 594) and caveolin-1 (green Alexa flour 488). Co-localization is visualized by the yellow color in merged images; ( B ) Subcellular localization of EGFR and caveolin-1 in cells pre-treated with inhibitors of carbon nanoparticle-specific signaling prior to particle or EGF exposure: SFK inhibitor PP2 (10 µM), 1 mM ectoine ( E ), 75 µM α-tocopherol (Toco), and 1 µg/mL filipin III (Fil). Co-localization is visualized by the yellow color in merged images; ( C ) Subcellular localization of caveolin-1 (red Alexa fluor 594) after exposure to carbon nanoparticles (CNP), carbon particles (CP), or hydrogen peroxide (H 2 O 2 ); ( D ) Quantification and representative Western blots of caveolin-1 in lipid raft fraction of RLE-6TN cells exposed to CNP (10 µg/cm 2 ). Raft and non-raft fraction were isolated from density gradients after ultracentrifugation. Pre-treatment of cells with α-tocopherol (Toco) was applied as an antioxidant strategy. GAPDH was used as a control protein not associated with lipid rafts. The bars in the graph represent the additive immune signals of raft and non-raft fractions, which was indicated in the representative original Western blots; ( E ) Quantification and representative Western-blot of EGFR phosphorylation at Tyr 845 . Nuclei were stained with DAPI (blue). Scale bars represent 20 µm; *, which was significantly different to PBS control ( p

    Article Snippet: Filipin III (from Streptomyces filipinensis , Sigma-Aldrich Chemie, Schnelldorf, Germany), PP2 (Calbiochem, Schwalbach, Germany), and DSP (dithiobis-succimidylpropionate, Thermo Scientific, Waltham, MA, USA) were solubilized in DMSO (dimethyl sulfoxide) and diluted in PBS to the indicated concentrations. α-tocopherol ( d -alpha-tocopherol succinate, semi-synthetic, Sigma-Aldrich Chemie, Schnelldorf, Germany) was solubilized in ethanol and further diluted in PBS before use.

    Techniques: Activation Assay, Western Blot, Isolation, Staining

    Caveolin-1 and EGFR co-localization as a feature of non-canonical EGFR activation. Epithelial cells (RLE-6TN) were exposed (5 min) to carbon nanoparticles (CNP), non-nano carbon particles (CP), each 10 µg/cm 2 , (50 µM) H 2 O 2 , (5 µM) C6 ceramide, or EGF (100 ng/mL), respectively. ( A ) Subcellular localization of EGFR (red Alexa flour 594) and caveolin-1 (green Alexa flour 488). Co-localization is visualized by the yellow color in merged images; ( B ) Subcellular localization of EGFR and caveolin-1 in cells pre-treated with inhibitors of carbon nanoparticle-specific signaling prior to particle or EGF exposure: SFK inhibitor PP2 (10 µM), 1 mM ectoine ( E ), 75 µM α-tocopherol (Toco), and 1 µg/mL filipin III (Fil). Co-localization is visualized by the yellow color in merged images; ( C ) Subcellular localization of caveolin-1 (red Alexa fluor 594) after exposure to carbon nanoparticles (CNP), carbon particles (CP), or hydrogen peroxide (H 2 O 2 ); ( D ) Quantification and representative Western blots of caveolin-1 in lipid raft fraction of RLE-6TN cells exposed to CNP (10 µg/cm 2 ). Raft and non-raft fraction were isolated from density gradients after ultracentrifugation. Pre-treatment of cells with α-tocopherol (Toco) was applied as an antioxidant strategy. GAPDH was used as a control protein not associated with lipid rafts. The bars in the graph represent the additive immune signals of raft and non-raft fractions, which was indicated in the representative original Western blots; ( E ) Quantification and representative Western-blot of EGFR phosphorylation at Tyr 845 . Nuclei were stained with DAPI (blue). Scale bars represent 20 µm; *, which was significantly different to PBS control ( p

    Journal: Nanomaterials

    Article Title: Non-Canonical Activation of the Epidermal Growth Factor Receptor by Carbon Nanoparticles

    doi: 10.3390/nano8040267

    Figure Lengend Snippet: Caveolin-1 and EGFR co-localization as a feature of non-canonical EGFR activation. Epithelial cells (RLE-6TN) were exposed (5 min) to carbon nanoparticles (CNP), non-nano carbon particles (CP), each 10 µg/cm 2 , (50 µM) H 2 O 2 , (5 µM) C6 ceramide, or EGF (100 ng/mL), respectively. ( A ) Subcellular localization of EGFR (red Alexa flour 594) and caveolin-1 (green Alexa flour 488). Co-localization is visualized by the yellow color in merged images; ( B ) Subcellular localization of EGFR and caveolin-1 in cells pre-treated with inhibitors of carbon nanoparticle-specific signaling prior to particle or EGF exposure: SFK inhibitor PP2 (10 µM), 1 mM ectoine ( E ), 75 µM α-tocopherol (Toco), and 1 µg/mL filipin III (Fil). Co-localization is visualized by the yellow color in merged images; ( C ) Subcellular localization of caveolin-1 (red Alexa fluor 594) after exposure to carbon nanoparticles (CNP), carbon particles (CP), or hydrogen peroxide (H 2 O 2 ); ( D ) Quantification and representative Western blots of caveolin-1 in lipid raft fraction of RLE-6TN cells exposed to CNP (10 µg/cm 2 ). Raft and non-raft fraction were isolated from density gradients after ultracentrifugation. Pre-treatment of cells with α-tocopherol (Toco) was applied as an antioxidant strategy. GAPDH was used as a control protein not associated with lipid rafts. The bars in the graph represent the additive immune signals of raft and non-raft fractions, which was indicated in the representative original Western blots; ( E ) Quantification and representative Western-blot of EGFR phosphorylation at Tyr 845 . Nuclei were stained with DAPI (blue). Scale bars represent 20 µm; *, which was significantly different to PBS control ( p

    Article Snippet: Filipin III (from Streptomyces filipinensis , Sigma-Aldrich Chemie, Schnelldorf, Germany), PP2 (Calbiochem, Schwalbach, Germany), and DSP (dithiobis-succimidylpropionate, Thermo Scientific, Waltham, MA, USA) were solubilized in DMSO (dimethyl sulfoxide) and diluted in PBS to the indicated concentrations. α-tocopherol (d -alpha-tocopherol succinate, semi-synthetic, Sigma-Aldrich Chemie, Schnelldorf, Germany) was solubilized in ethanol and further diluted in PBS before use.

    Techniques: Activation Assay, Western Blot, Isolation, Staining