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Biomol GmbH filipin iii
IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with <t>Filipin</t> <t>III</t> in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.
Filipin Iii, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/filipin iii/product/Biomol GmbH
Average 94 stars, based on 2 article reviews
Price from $9.99 to $1999.99
filipin iii - by Bioz Stars, 2020-04
94/100 stars

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1) Product Images from "IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis"

Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis

Journal: Scientific Reports

doi: 10.1038/s41598-018-19476-x

IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.
Figure Legend Snippet: IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

Techniques Used: Infection, Immunofluorescence, Staining, Expressing

2) Product Images from "IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis"

Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis

Journal: Scientific Reports

doi: 10.1038/s41598-018-19476-x

IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.
Figure Legend Snippet: IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

Techniques Used: Infection, Immunofluorescence, Staining, Expressing

Related Articles

Staining:

Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
Article Snippet: .. To stain with Filipin III (Biomol), stock solution was diluted to 1:100 with PBS/BSA 3% and incubated for 2 h at RT in the dark. .. Cells were then visualized using Leica DMR microscope (Leica Microsystems, Wetzlar, Germany) under UV light filter set (340–380 nm) and captured at 40× magnification.

Incubation:

Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
Article Snippet: .. To stain with Filipin III (Biomol), stock solution was diluted to 1:100 with PBS/BSA 3% and incubated for 2 h at RT in the dark. .. Cells were then visualized using Leica DMR microscope (Leica Microsystems, Wetzlar, Germany) under UV light filter set (340–380 nm) and captured at 40× magnification.

Infection:

Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
Article Snippet: Cells were then infected with Mtb or left uninfected and fixed at 24 h and 48 h with 4% paraformaldehyde (Electron Microscopy Sciences, Munich, Germany) for 1 h and then washed with PBS three times. .. To stain with Filipin III (Biomol), stock solution was diluted to 1:100 with PBS/BSA 3% and incubated for 2 h at RT in the dark.

Microscopy:

Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
Article Snippet: To stain with Filipin III (Biomol), stock solution was diluted to 1:100 with PBS/BSA 3% and incubated for 2 h at RT in the dark. .. Cells were then visualized using Leica DMR microscope (Leica Microsystems, Wetzlar, Germany) under UV light filter set (340–380 nm) and captured at 40× magnification.

Electron Microscopy:

Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
Article Snippet: Cells were then infected with Mtb or left uninfected and fixed at 24 h and 48 h with 4% paraformaldehyde (Electron Microscopy Sciences, Munich, Germany) for 1 h and then washed with PBS three times. .. To stain with Filipin III (Biomol), stock solution was diluted to 1:100 with PBS/BSA 3% and incubated for 2 h at RT in the dark.

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    Biomol GmbH filipin iii
    IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with <t>Filipin</t> <t>III</t> in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.
    Filipin Iii, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin iii/product/Biomol GmbH
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    filipin iii - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Journal: Scientific Reports

    Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis

    doi: 10.1038/s41598-018-19476-x

    Figure Lengend Snippet: IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Article Snippet: To stain with Filipin III (Biomol), stock solution was diluted to 1:100 with PBS/BSA 3% and incubated for 2 h at RT in the dark.

    Techniques: Infection, Immunofluorescence, Staining, Expressing

    IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Journal: Scientific Reports

    Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis

    doi: 10.1038/s41598-018-19476-x

    Figure Lengend Snippet: IL-36 signaling modulates cholesterol metabolism in Mtb -infected macrophage. ( A ) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. ( B ) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb -infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). ( C ) mRNA expression of CD36 , LDLR , SRA in Mtb -infected scramble versus IL36R KD cells. ( D ) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. ( E ) SREBP2 protein expression in Mtb -infected scramble and IL-36 deficient cells. ( F ) mRNA expression of several cholesterol synthesis genes in Mtb -infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. ( A , C , D , F ) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. ( B and E ) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Article Snippet: To stain with Filipin III (Biomol), stock solution was diluted to 1:100 with PBS/BSA 3% and incubated for 2 h at RT in the dark.

    Techniques: Infection, Immunofluorescence, Staining, Expressing