filipin iii signals  (BioVision)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Filipin III
    Description:
    Filipin III is a polyene macrolide antibiotic isolated from Streptomyces filipinensis It may be used in a double staining procedure for the detection of lipoproteins Binds specifically to cholesterol forming a fluorescent complex which may be used for staining Filipin III disrupts the lipid raft caveolae endocytosis pathway Prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release
    Catalog Number:
    2744-500
    Price:
    None
    Size:
    500 µg
    Category:
    Biochemicals
    Quantity:
    1
    Buy from Supplier


    Structured Review

    BioVision filipin iii signals
    Filipin III
    Filipin III is a polyene macrolide antibiotic isolated from Streptomyces filipinensis It may be used in a double staining procedure for the detection of lipoproteins Binds specifically to cholesterol forming a fluorescent complex which may be used for staining Filipin III disrupts the lipid raft caveolae endocytosis pathway Prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release
    https://www.bioz.com/result/filipin iii signals/product/BioVision
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin iii signals - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Modulating cancer cell survival by targeting intracellular cholesterol transport"

    Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport

    Journal: British Journal of Cancer

    doi: 10.1038/bjc.2017.200

    ASM inhibitors induce melanoma-specific cell death by inhibiting intracellular cholesterol transport. ( A ) fluorescence microscopy of intracellular cholesterol localisation following knockdown of SMPD1 (ASM1) as detected by Filipin-III staining (arrows show accumulated cholesterol); ( B ) the viability of UACC 903 and 1205 Lu melanoma cells following knockdown of SMPD1; ( C ) IC 50 values of various melanoma cell lines and FF2441 fibroblasts treated with ASM inhibitors. Dose–response curves were drawn in OriginPro (OriginLab) using Levenberg Marquardt algorithm; ( D ) viability of UACC 903 and FF2441 cells following 24 h of treatment with increasing concentrations of ASM inhibitors; ( E ) viability of melanoma cells treated with various ASM inhibitors in the absence or presence of v-ATPase inhibitor, Bafilomycin-A1; ( F ) intracellular cholesterol localisation following leelamine, U18666A or ASM inhibitor treatments as detected by Filipin-III staining. (lower) Co-localisation of RFP-tagged lysosomal LAMP1 protein with cholesterol; ( G ) LDL treatment protects UACC 903 cells from ASM inhibitor-mediated cell death; ( H ) Venn diagram showing number of significantly altered genes identified by RNA-sequencing of UACC 903 cells treated with ASM inhibitors (left); and enrichment analysis of significantly altered 177 genes; ( I ) expression level of cholesterol synthesis genes following ASM treatment as detected by RNA-sequencing; ( J ) cholesterol synthesis pathway. White highlighted genes were identified as significantly deregulated by RNA-sequencing; ( K ) distribution of log-2-fold change in expression levels of cholesterol synthesis genes identified by microarray analysis following treatment of MCF7 cells with ASM inhibitors.
    Figure Legend Snippet: ASM inhibitors induce melanoma-specific cell death by inhibiting intracellular cholesterol transport. ( A ) fluorescence microscopy of intracellular cholesterol localisation following knockdown of SMPD1 (ASM1) as detected by Filipin-III staining (arrows show accumulated cholesterol); ( B ) the viability of UACC 903 and 1205 Lu melanoma cells following knockdown of SMPD1; ( C ) IC 50 values of various melanoma cell lines and FF2441 fibroblasts treated with ASM inhibitors. Dose–response curves were drawn in OriginPro (OriginLab) using Levenberg Marquardt algorithm; ( D ) viability of UACC 903 and FF2441 cells following 24 h of treatment with increasing concentrations of ASM inhibitors; ( E ) viability of melanoma cells treated with various ASM inhibitors in the absence or presence of v-ATPase inhibitor, Bafilomycin-A1; ( F ) intracellular cholesterol localisation following leelamine, U18666A or ASM inhibitor treatments as detected by Filipin-III staining. (lower) Co-localisation of RFP-tagged lysosomal LAMP1 protein with cholesterol; ( G ) LDL treatment protects UACC 903 cells from ASM inhibitor-mediated cell death; ( H ) Venn diagram showing number of significantly altered genes identified by RNA-sequencing of UACC 903 cells treated with ASM inhibitors (left); and enrichment analysis of significantly altered 177 genes; ( I ) expression level of cholesterol synthesis genes following ASM treatment as detected by RNA-sequencing; ( J ) cholesterol synthesis pathway. White highlighted genes were identified as significantly deregulated by RNA-sequencing; ( K ) distribution of log-2-fold change in expression levels of cholesterol synthesis genes identified by microarray analysis following treatment of MCF7 cells with ASM inhibitors.

    Techniques Used: Fluorescence, Microscopy, Staining, RNA Sequencing Assay, Expressing, Microarray

    Related Articles

    Staining:

    Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport
    Article Snippet: Cholesterol localisation assay Localisation of intracellular cholesterol was detected through Filipin-III staining of cells using the Cayman’s Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). .. Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA).

    Detection Assay:

    Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport
    Article Snippet: Cholesterol localisation assay Localisation of intracellular cholesterol was detected through Filipin-III staining of cells using the Cayman’s Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). .. Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA).

    Expressing:

    Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport
    Article Snippet: Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA). .. An RFP-tagged LAMP1 expressing UACC 903 cell line was created using LAMP1-mRFP-FLAG plasmid (Addgene plasmid #34611) ( ).

    Plasmid Preparation:

    Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport
    Article Snippet: Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA). .. An RFP-tagged LAMP1 expressing UACC 903 cell line was created using LAMP1-mRFP-FLAG plasmid (Addgene plasmid #34611) ( ).

    Software:

    Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport
    Article Snippet: .. Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA). .. An RFP-tagged LAMP1 expressing UACC 903 cell line was created using LAMP1-mRFP-FLAG plasmid (Addgene plasmid #34611) ( ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    BioVision filipin iii signals
    ASM inhibitors induce melanoma-specific cell death by inhibiting intracellular cholesterol transport. ( A ) fluorescence microscopy of intracellular cholesterol localisation following knockdown of SMPD1 (ASM1) as detected by <t>Filipin-III</t> staining (arrows show accumulated cholesterol); ( B ) the viability of UACC 903 and 1205 Lu melanoma cells following knockdown of SMPD1; ( C ) IC 50 values of various melanoma cell lines and FF2441 fibroblasts treated with ASM inhibitors. Dose–response curves were drawn in OriginPro (OriginLab) using Levenberg Marquardt algorithm; ( D ) viability of UACC 903 and FF2441 cells following 24 h of treatment with increasing concentrations of ASM inhibitors; ( E ) viability of melanoma cells treated with various ASM inhibitors in the absence or presence of v-ATPase inhibitor, Bafilomycin-A1; ( F ) intracellular cholesterol localisation following leelamine, U18666A or ASM inhibitor treatments as detected by Filipin-III staining. (lower) Co-localisation of RFP-tagged lysosomal LAMP1 protein with cholesterol; ( G ) LDL treatment protects UACC 903 cells from ASM inhibitor-mediated cell death; ( H ) Venn diagram showing number of significantly altered genes identified by RNA-sequencing of UACC 903 cells treated with ASM inhibitors (left); and enrichment analysis of significantly altered 177 genes; ( I ) expression level of cholesterol synthesis genes following ASM treatment as detected by RNA-sequencing; ( J ) cholesterol synthesis pathway. White highlighted genes were identified as significantly deregulated by RNA-sequencing; ( K ) distribution of log-2-fold change in expression levels of cholesterol synthesis genes identified by microarray analysis following treatment of MCF7 cells with ASM inhibitors.
    Filipin Iii Signals, supplied by BioVision, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin iii signals/product/BioVision
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin iii signals - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    ASM inhibitors induce melanoma-specific cell death by inhibiting intracellular cholesterol transport. ( A ) fluorescence microscopy of intracellular cholesterol localisation following knockdown of SMPD1 (ASM1) as detected by Filipin-III staining (arrows show accumulated cholesterol); ( B ) the viability of UACC 903 and 1205 Lu melanoma cells following knockdown of SMPD1; ( C ) IC 50 values of various melanoma cell lines and FF2441 fibroblasts treated with ASM inhibitors. Dose–response curves were drawn in OriginPro (OriginLab) using Levenberg Marquardt algorithm; ( D ) viability of UACC 903 and FF2441 cells following 24 h of treatment with increasing concentrations of ASM inhibitors; ( E ) viability of melanoma cells treated with various ASM inhibitors in the absence or presence of v-ATPase inhibitor, Bafilomycin-A1; ( F ) intracellular cholesterol localisation following leelamine, U18666A or ASM inhibitor treatments as detected by Filipin-III staining. (lower) Co-localisation of RFP-tagged lysosomal LAMP1 protein with cholesterol; ( G ) LDL treatment protects UACC 903 cells from ASM inhibitor-mediated cell death; ( H ) Venn diagram showing number of significantly altered genes identified by RNA-sequencing of UACC 903 cells treated with ASM inhibitors (left); and enrichment analysis of significantly altered 177 genes; ( I ) expression level of cholesterol synthesis genes following ASM treatment as detected by RNA-sequencing; ( J ) cholesterol synthesis pathway. White highlighted genes were identified as significantly deregulated by RNA-sequencing; ( K ) distribution of log-2-fold change in expression levels of cholesterol synthesis genes identified by microarray analysis following treatment of MCF7 cells with ASM inhibitors.

    Journal: British Journal of Cancer

    Article Title: Modulating cancer cell survival by targeting intracellular cholesterol transport

    doi: 10.1038/bjc.2017.200

    Figure Lengend Snippet: ASM inhibitors induce melanoma-specific cell death by inhibiting intracellular cholesterol transport. ( A ) fluorescence microscopy of intracellular cholesterol localisation following knockdown of SMPD1 (ASM1) as detected by Filipin-III staining (arrows show accumulated cholesterol); ( B ) the viability of UACC 903 and 1205 Lu melanoma cells following knockdown of SMPD1; ( C ) IC 50 values of various melanoma cell lines and FF2441 fibroblasts treated with ASM inhibitors. Dose–response curves were drawn in OriginPro (OriginLab) using Levenberg Marquardt algorithm; ( D ) viability of UACC 903 and FF2441 cells following 24 h of treatment with increasing concentrations of ASM inhibitors; ( E ) viability of melanoma cells treated with various ASM inhibitors in the absence or presence of v-ATPase inhibitor, Bafilomycin-A1; ( F ) intracellular cholesterol localisation following leelamine, U18666A or ASM inhibitor treatments as detected by Filipin-III staining. (lower) Co-localisation of RFP-tagged lysosomal LAMP1 protein with cholesterol; ( G ) LDL treatment protects UACC 903 cells from ASM inhibitor-mediated cell death; ( H ) Venn diagram showing number of significantly altered genes identified by RNA-sequencing of UACC 903 cells treated with ASM inhibitors (left); and enrichment analysis of significantly altered 177 genes; ( I ) expression level of cholesterol synthesis genes following ASM treatment as detected by RNA-sequencing; ( J ) cholesterol synthesis pathway. White highlighted genes were identified as significantly deregulated by RNA-sequencing; ( K ) distribution of log-2-fold change in expression levels of cholesterol synthesis genes identified by microarray analysis following treatment of MCF7 cells with ASM inhibitors.

    Article Snippet: Lysosomal/late endosomal localisation of cholesterol was assessed by co-localisation of LAMP1-RFP and Filipin-III signals using iVision software (BioVision Technologies, Chester Springs, PA, USA).

    Techniques: Fluorescence, Microscopy, Staining, RNA Sequencing Assay, Expressing, Microarray