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Genmed filipin fluorescence staining kit
Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, <t>Filipin</t> staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p
Filipin Fluorescence Staining Kit, supplied by Genmed, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/filipin fluorescence staining kit/product/Genmed
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
filipin fluorescence staining kit - by Bioz Stars, 2020-04
91/100 stars

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1) Product Images from "Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism"

Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism

Journal: Scientific Reports

doi: 10.1038/srep31381

Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, Filipin staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p
Figure Legend Snippet: Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, Filipin staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p

Techniques Used: Mouse Assay, Cholesterol Assay, Staining, Real-time Polymerase Chain Reaction

Treatment with 4-PBA alleviated lipid metabolic disorders in SCH mice. (A) Levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the serum of all mice were determined after 4-PBA was administered for 4 weeks (n = 6–12). (B) Hepatic TC contents were detected using a cholesterol assay kit after 4-PBA was administered for 4 weeks (n = 6–12). (C) Hepatic TG contents were detected using a triglyceride assay kit after 4-PBA was administered for 4 weeks (n = 6–13). (D) Filipin staining was used to measure hepatic cholesterol deposition after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (E) Oil red O staining was used to measure hepatic TG accumulation after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (F) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 18th week (n = 4–6). The data are presented as the mean ± SD. *p
Figure Legend Snippet: Treatment with 4-PBA alleviated lipid metabolic disorders in SCH mice. (A) Levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the serum of all mice were determined after 4-PBA was administered for 4 weeks (n = 6–12). (B) Hepatic TC contents were detected using a cholesterol assay kit after 4-PBA was administered for 4 weeks (n = 6–12). (C) Hepatic TG contents were detected using a triglyceride assay kit after 4-PBA was administered for 4 weeks (n = 6–13). (D) Filipin staining was used to measure hepatic cholesterol deposition after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (E) Oil red O staining was used to measure hepatic TG accumulation after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (F) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 18th week (n = 4–6). The data are presented as the mean ± SD. *p

Techniques Used: Mouse Assay, Cholesterol Assay, Staining, Real-time Polymerase Chain Reaction

Related Articles

Staining:

Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism
Article Snippet: .. Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol. .. These images were acquired by a fluorescence microscope (Axio Cam HRC, Zeiss, Germany) at 359/461 (excitation/emission) for filipin.

Fluorescence:

Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism
Article Snippet: .. Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol. .. These images were acquired by a fluorescence microscope (Axio Cam HRC, Zeiss, Germany) at 359/461 (excitation/emission) for filipin.

Microscopy:

Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism
Article Snippet: Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol. .. These images were acquired by a fluorescence microscope (Axio Cam HRC, Zeiss, Germany) at 359/461 (excitation/emission) for filipin.

Chick Chorioallantoic Membrane Assay:

Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism
Article Snippet: Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol. .. These images were acquired by a fluorescence microscope (Axio Cam HRC, Zeiss, Germany) at 359/461 (excitation/emission) for filipin.

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    Genmed filipin fluorescence staining kit
    Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, <t>Filipin</t> staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p
    Filipin Fluorescence Staining Kit, supplied by Genmed, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin fluorescence staining kit/product/Genmed
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin fluorescence staining kit - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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    Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, Filipin staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p

    Journal: Scientific Reports

    Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism

    doi: 10.1038/srep31381

    Figure Lengend Snippet: Hyperlipidemia and hepatic lipid metabolic disorders in the SCH mice. Male C57/BL6 mice were administered methimazole (MMI, 0.08 mg/kg·BW·d, SCH group, n = 6–8) or a corresponding volume of vehicle (control group, n = 6–8) for 12 weeks or 16 weeks. (A) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 12th week. (B) Levels of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides (TG) in the serum of all mice were determined in the 16th week. (C) Liver TC contents were measured using a cholesterol assay kit in the 12th week and the 16th week. (D) i, Filipin staining was used to measure liver cholesterol deposition in the 12th week. ii, Filipin staining was used to measure liver cholesterol deposition in the 16th week. Original magnification: 200×. (E) Liver TG contents were measured using a triglyceride assay kit in the 12th week and the 16th week. (F) i, Oil red O staining was used to measure liver TG accumulation in the 12th week. ii, Oil red O staining was used to measure liver TG accumulation in the 16th week. Original magnification: 200×. (G) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 12th week. The data are presented as the mean ± SD. *p

    Article Snippet: Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol.

    Techniques: Mouse Assay, Cholesterol Assay, Staining, Real-time Polymerase Chain Reaction

    Treatment with 4-PBA alleviated lipid metabolic disorders in SCH mice. (A) Levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the serum of all mice were determined after 4-PBA was administered for 4 weeks (n = 6–12). (B) Hepatic TC contents were detected using a cholesterol assay kit after 4-PBA was administered for 4 weeks (n = 6–12). (C) Hepatic TG contents were detected using a triglyceride assay kit after 4-PBA was administered for 4 weeks (n = 6–13). (D) Filipin staining was used to measure hepatic cholesterol deposition after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (E) Oil red O staining was used to measure hepatic TG accumulation after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (F) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 18th week (n = 4–6). The data are presented as the mean ± SD. *p

    Journal: Scientific Reports

    Article Title: Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism

    doi: 10.1038/srep31381

    Figure Lengend Snippet: Treatment with 4-PBA alleviated lipid metabolic disorders in SCH mice. (A) Levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the serum of all mice were determined after 4-PBA was administered for 4 weeks (n = 6–12). (B) Hepatic TC contents were detected using a cholesterol assay kit after 4-PBA was administered for 4 weeks (n = 6–12). (C) Hepatic TG contents were detected using a triglyceride assay kit after 4-PBA was administered for 4 weeks (n = 6–13). (D) Filipin staining was used to measure hepatic cholesterol deposition after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (E) Oil red O staining was used to measure hepatic TG accumulation after 4-PBA was administered for 4 weeks (n = 3). Original magnification: 200×. (F) Real-time PCR analysis of different lipid metabolism-related genes in the liver was performed in the 18th week (n = 4–6). The data are presented as the mean ± SD. *p

    Article Snippet: Frozen sections of the liver (5 μm) were stained using a filipin fluorescence staining kit (Genmed Scientifics Inc., USA), according to the manufacturer’s recommended protocol.

    Techniques: Mouse Assay, Cholesterol Assay, Staining, Real-time Polymerase Chain Reaction